Determinacion de Azucares Reductores Por El Metodo Del DNS

UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. EAP. INGENIERIA INGENI ERIA AGROINDUSTRIAL AG ROINDUSTRIAL

ANALISIS INSTRUMENTAL DE PRODUCTOS AGROINDUSTRIALES

UNIVERSIDAD NACIONAL DEL SANA FACUL ACU LTAD DE INGENIER INGE NIERIA IA E.A.P: E.A.P : “INGENIERIA “INGENI ERIA AGROINDUST AGR OINDUSTRIAL RIAL” ” TEMA: DETERMINACIÓN DETERMINACIÓN DE AZUCARES REDUCTORES POR EL MÉTODO DE DNS. CURSO: ANÁLISIS INSTRUMENTAL DE PRODUCTOS AGROINDUSTRIALES. DOCENTES: GILBERT RODRÍGUEZ PAUCAR. ALUMNOS:

FELIPE RODRÍGUEZ MITZY. ISIQUE ISIQ UE VALVER ALVERDE DE DAVID. DAVID. SILVA NATIVIDAD JUAN JOSÉ CICLO: Vl NVO CHIMBOTE - PERÚ



PRACTICA Nº 03: DETERMINACION DE AZUCARES REDUCTORES POR ESPECTROFOTOMETRIA METODO DNS! I.

INTRODUCCION

Hoy ! "#$ %$ &!"'()*&$ %&+o**$ y $%&,!)&+&$ ')&%&-$ $*&o( )&/o( " ,0)o"o( $!$%#)&+o( /$*$ %$ ")*,&!$+&1! $-2+$* y $%+o3o% ! %o( %&+o %&+o**( y4o y4o 55& 55&"$ "$(( $ ')&% ')&%&&-$* $* +o,o +o,o ,"& ,"&o o " !) !)$. $. U!o U!o " %o( %o( ,0)o"o( ,6( $+o*"( y +o! ,7o* *('%)$"o( ! %$ ")*,&!$+&1! " %o( $-'+$*( +o! +$*6+)* *"'+)o*8 ( % ,0)o"o 9DNS:8 ,0)o"o ;'



PRACTICA Nº 03: DETERMINACION DE AZUCARES REDUCTORES POR ESPECTROFOTOMETRIA METODO DNS! I.

INTRODUCCION

Hoy ! "#$ %$ &!"'()*&$ %&+o**$ y $%&,!)&+&$ ')&%&-$ $*&o( )&/o( " ,0)o"o( $!$%#)&+o( /$*$ %$ ")*,&!$+&1! $-2+$* y $%+o3o% ! %o( %&+o %&+o**( y4o y4o 55& 55&"$ "$(( $ ')&% ')&%&&-$* $* +o,o +o,o ,"& ,"&o o " !) !)$. $. U!o U!o " %o( %o( ,0)o"o( ,6( $+o*"( y +o! ,7o* *('%)$"o( ! %$ ")*,&!$+&1! " %o( $-'+$*( +o! +$*6+)* *"'+)o*8 ( % ,0)o"o 9DNS:8 ,0)o"o ;'



3$ ('<*&"o $*&$( ,o"&=+$+&o!( ,o"&=+$+&o!( $ )*$0( " %o( $>o( /$*$ $"+'$*( $"+'$*( $% $!6%&(&( " "&<*!)( ,$)*&$%( y (' /*&!+&/$% !)$7$ *$"&+$ ! (' $%) $%)$ (!( (!(&&5&% 5&%&"$" &"$" y /*o" /*o"'+ '+))&& &&"$" "$" "5& "5&"o "o $ ;' ;' ( '! ,0) ,0)o"o o"o (/+)*o
II.

FUNDAMENTO TEORICO "#$% &' l( )l$*+'(,

L$ %'+o($ ( '! +$*5o3&"*$)o (&,/%8 y ( %$ '!&"$" " !*#$ ,6( 56(&+$ /$*$ %$( /%$!)$( y %o( $!&,$%(. E()*'+)'*$%,!)8 %$ %'+o($ ( '! $-2+$* " (&( +$*5o!o( ;' )&! '! $%"3#"o ! '! @)*,o ! ('


L$ %'+o($ !o ( % 2!&+o $-2+$* *"'+)o*. C'$%;'&* $-2+$* ;' ($ ()*'+)'*$%, ()*'+)'*$%,!) !) +$/$- " "o!$* %+)*o!( %+)*o!( $ '!$ (o%'+&1! " B!"&+) B!"&+) 9o '! *$+ *$+)& )&o o (&,& (&,&%$ %$*: *: +$ +$ "!) "!)*o *o " ()$ ()$ +$) +$)o* o*#$ #$.. Lo( Lo( $-2+ $-2+$* $*( ( 5$($ 5$($"o( "o( ! $%" $%"3# 3#"o "o(( ;' ;' ()6 ()6! ! ! ('
III .

OB2ETIVOS •

•

•

IV. IV.

E()$ E()$5% 5%+ +* * %$ %o! %o!&) &)'" '" " o!"$ o!"$ " ,6@& ,6@&,$ ,$ $5(o* $5(o*+& +&1! 1! /$*$ /$*$ %$( %$( "&<*!)( (o%'+&o!( +o%o*$"$(. C'$!)&=+$* %o( $-'+$*( *"'+)o*( /*(!)( ! $%&,!)o(. D($**o%%$* % /*o+"&,&!)o *(/+)&o /$*$ %$ ")*,&!$+&1! " $-'+$*( *"'+)o*( $ /$*)&* " '!$ (o%'+&1! /$)*1! " %'+o($8 ;' /*,&)&*6 *$%&-$* y $7'()$* '!$ +'*$ " $5(o*5$!+&$8 /o* % ,0)o"o "% 6+&"o 8 "&!&)*o ($%&+#%&+o 9DNS:.

MATERIAL IALES  METODOS DOS

A! M(1&/ M(1&/4(l 4(l&' &' • • •

E(/+)*o
B! R&(*145+ R&(*145+ DNS : •

T$*)*$)o T$*)*$)o " (o"&o y /o)$(&o


UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. EAP. INGENIERIA INGENI ERIA AGROINDUSTRIAL AG ROINDUSTRIAL • • •

H&"*1@&"o " (o"&o M)$5&('%=)o " (o"&o R$+)&o DNS

P*/$*$+&1! "% *$+)&o DNS S ,-+%$ y "&('% !  ,% " $'$ "()&%$"$ K * " N$OH y  * " )$*)*$)o " (o"&o y /o)$(&o. Po()*&o*,!) ( $*$!  * " 6+&"o 8 "&!o)*$($%&+&%&+o 5$7o +$%!)$,&!)o8 ( $
C! M%1+ M%1++ +' ':

. A ,'( ,'()* )*$( $( +o%o +o%o**$"$( $"$( *$%& *$%&-$* -$*% %(( % 5$**& 5$**&"o "o " %+) %+)'*$ '*$(( " $5(o*5$!+&$ "("  $  !,8 %'o ")*,&!$* (' ,6@&,$ $5(o*5$!+&$ . P*/ P*/$* $*$* $* '! ()6 ()6!" !"$* $* " . . ,4, ,4,%% " %'+ %'+o( o($ $ *$%& $%&-$ -$** %$( %$( "&%'+&o!( /$*$ o5)!* +o!+!)*$+&o!( " . 8 . 8 . 8 .K y . ,4,%.

V.

CALC CALCUL ULOS OS  RESU RESUL LTADOS ADOS

A5(o*5$!+&$ o5)!&"$ ! "o( "&<*!)( ;'&/o( PRIMER GRUPO

SEGUNDO GRUPO

C+6*&61/(*476 8)98l! 0

2HON

IITA IITA

2HON

IITA IITA



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0;

8

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0<

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0=

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0>

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UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL

A6(l4(6+ PRIMER GRUPO:

GRAFICA Nº 0;

C+6*&61/(* PRIMER 476 GRUPO 8)98l! 2HON IITA 0   0; 8 8K   0< 8 8   0= 8 8  0> 8 8   0? 8 8  ;0 8 8   ;< 8 8  ;= 8 8K  




HALLANDO CANTIDAD DE AZUCARES REDUCTORES: •

NARANJA

J'o

A'$  ,%

. ,%

 ,% DNS  ,%  ,% $'$

P/&@(/(*476 & 8$&'1/( ºBRI  ;<

S'/o!&!"o 100 ml

0.5 ml

0.5 ml × 12000 mg=100 ml x

x =1.2

S !+'!)*$ ! % *$!o *;'*&"o

2HON:

12000 mg

x



L&*1$/( & ('+/(6*4(: 0.<;= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.3953 X −0.0075 A = 0.3953 C −0.0075

C

0.5621

=

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

1000 mg

g

mg ml

ENTONCES LA CANTIDAD DE AZUCARES REDUCTORES SERA

100

10.506

mg ml

100

mg ml

mg mg × 0.5621=10.506 x ml ml

0.5621

x



x =5.35 de azucares reductores

IITA: L&*1$/( & ('+/(6*4(: 0.33 P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.6603 X −0.0483

A = 0.6603 C −0.0483 C =0. 5849

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

1000 mg

g

mg ml

ENTONCES LA CANTIDAD DE AZUCARES REDUCTORES SERA 10.506

mg ml

0.5849


UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 100

100

mg ml

x

mg mg × 0.57 =10.506 x ml ml

x =5.57 de azucares reductores •

PIA

J'o

A'$  ,%

. ,%

 ,% DNS  ,%  ,% $'$

P/&@(/(*476 & 8$&'1/( ºBRI  ;<.<

S'/o!&!"o 100 ml

0.5 ml

12200 mg

x



0.5 ml × 12200 mg=100 ml x

x =1.22

S !+'!)*$ ! % *$!o *;'*&"o

2HON: L&*1$/( & ('+/(6*4(: 0.;3> P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.3953 X −0.0075 A = 0.3953 C −0.0075

C =0. 3721

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.484

×

1000 mg

g

mg ml


UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 10.484

mg ml

100

100

mg ml

ANALISIS INSTRUMENTAL DE PRODUCTOS AGROINDUSTRIALES 0.3721

x

mg mg × 0.3721=10.484 x ml ml


IITA: L&*1$/( & ('+/(6*4(: 0.<<> P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.6603 X −0.0483

A = 0.6603 C −0.0483 C =0.4168

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$ 0.5242 g 50 ml

×

1000 mg

g



mg ml


mg ml

100

100

mg × 0.416 ml

=

10.484

0.4168

mg ml

x

mg x ml


•

CARAMBOLA

J'o

A'$  ,%

 ,%

 ,% DNS  ,%  ,% $'$

P/&@(/(*476 & 8$&'1/(



ºBRI  ;<.<

S'/o!&!"o 100 ml

0.5 ml

12200 mg

x

0.5 ml × 12200 mg=100 ml x

x =1.22

S !+'!)*$ ! % *$!o *;'*&"o

2HON: L&*1$/( & ('+/(6*4(: 0.<= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.3953 X −0.0075 A = 0.3953 C −0.0075

C =0.7640

M'()*$  ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml× ρ

1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$



g 1000 mg × g 50 ml

1.020

20.4

mg ml

ENTONCES LA CANTIDAD DE AZUCARES REDUCTORES SERA mg ml

.

100

100

mg ml

0.7640

x

mg mg × 0.7640=20.4 x ml ml


IITA: L&*1$/( & ('+/(6*4(: 0.=? P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.6603 X −0.0483

A = 0.6603 C −0.0483 C =0.7978

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml×ρ


UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$ g 1000 mg × 50 ml g

1.020

20.4

mg ml


mg ml

100

100

0.7978

mg ml

mg mg × 0.7840=20.4 x ml ml

x =3.91 de azucaresreductores

D$)o( +o**&"o(

C+6*&61/(* PRIMER 476 GRUPO 8)98l! 2HON IITA 0; 8 8K   0< 8 8   0= 8 8  0> 8 8   0? 8 8K ;0 8 8   ;< 8 8  ;= 8 8K

x



A'. VS. C+6*&61/(*4+6  . .K . . ABSORBANCIA . . . . .  

<9@:  .@  . R   Jo3! L&!$* 9Jo3!: IITA L&!$* 9IITA:

<9@:  .@  . R  

.

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CONCENTRACION

GRAFICA Nº 0<

HALLANDO CANTIDAD DE AZUCARES REDUCTORES:

NARAN2A

2HON: L&*1$/( & ('+/(6*4(: 0.<;= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y

=

0.401 X −0.0075



A = 0.401 C −0.0075 C =0 .5541

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

1000 mg

g

mg ml


mg ml

100

mg ml

0.5541

x



mg mg × 0.5541=10.506 x ml ml


IITA: L&*1$/( & ('+/(6*4(: 0.33 P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.6884 X −0.0612

A

=

0.6884 C −0.0612

C =0. 5798

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

×

1000 mg

g



mg ml


100

10.506

mg ml

100

mg ml

mg mg × 0.5798=10.506 x ml ml


PIA

2HON:

0.5798

x



L&*1$/( & ('+/(6*4(: 0.;3> P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y

=

0.401 X −0.0075

A = 0.401 C −0.0075

C =0. 3668

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.484

×

1000 mg

g

mg ml


mg ml

0.3668


100

mg ml

ANALISIS INSTRUMENTAL DE PRODUCTOS AGROINDUSTRIALES x

mg mg × 0.3721=10.484 x ml ml


IITA: L&*1$/( & ('+/(6*4(: 0.<<>

P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.6884 x −0.0612

A

=

0.6884 C −0.0612

C =0.4185

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$

UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 0.5242 g 50 ml

10.484

×


1000 mg

g

mg ml


mg ml

100

100

mg ml

mg mg × 0.4185=10.484 x ml ml


CARAMBOLA

0.4185

x



2HON: L&*1$/( & ('+/(6*4(: 0.<= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y

=

0.401 X −0.0075

A = 0.401 C −0.0075

C

0.7531

=

M'()*$  ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml× ρ

1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$ g 1000 mg × 50 ml g

1.020

20.4

mg ml



.

100

100

mg × 0.7531 ml

20.4

=

mg ml

mg ml

ANALISIS INSTRUMENTAL DE PRODUCTOS AGROINDUSTRIALES 0.7531

x

mg x ml


IITA: L&*1$/( & ('+/(6*4(: 0.=?

P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.6884 x −0.0612

A

=

0.6884 C −0.0612

C =0.7840

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml×ρ

1 ml × 1.020

1.020 g

g ml



Co!+!)*$+&1! " %$ =o%$ g 1000 mg × g 50 ml

1.020

20.4

mg ml


mg ml

100

100

mg ml

mg mg × 0.7840=20.4 x ml ml


0.7840

x


•

A!$%&-$!"o SEGUNDO GRUPO

C+6*&61/(*476 8)98l! 0

SEGUNDO GRUPO 2HON IITA  

0;

8

8

0<

8

8

0=

8

8

0>

8KKK

8K

0?

8

8K

;0

8K

8

;<

8K

8

;=

8K

8




A'. V'. C+6*&61/(*4+6 . .

<9@:  .K@  . R  .

.K <9@:9JHON:  .K@  .INSTITUTO JHON L&!$* . ABSORBANCIA R  . .

L&!$* 9INSTITUTO:

. . .

.

.

.

.K

.

.

.

CONCENTRACION

GRAFICA Nº 03 HALLANDO CANTIDAD DE AZUCARES REDUCTORES:

NARAN2A

2HON: L&*1$/( & ('+/(6*4(: 0.<;= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.4849 X + 0.0071

A = 0.4849 C + 0.0071 C =0 .4281

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o(

.

UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 0.5 ml×


ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

1000 mg

g

mg ml


100

10.506

mg ml

100

mg ml

mg mg × 0.4281=10.506 x ml ml


IITA:

0.4281

x



L&*1$/( & ('+/(6*4(: 0.33 P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.7968 X −0.0244 A = 0.7968 C −0.0244

C =0. 4547

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

1000 mg

g

mg ml


mg ml

0.4547


100

mg ml

ANALISIS INSTRUMENTAL DE PRODUCTOS AGROINDUSTRIALES x

mg mg × 0.4547= 10.506 x ml ml


PIA

2HON: L&*1$/( & ('+/(6*4(: 0.;3> P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.4849 X + 0.0071 A = 0.4849 C + 0.0071



C =0. 2733

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.484

×

1000 mg

g

mg ml


mg ml

100

100

mg ml

mg mg × 0.2733=10.484 x ml ml

0.3668

x




IITA: L&*1$/( & ('+/(6*4(: 0.<<>

P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.7968 X −0.0244

A

=

0.7968 −0.0244

C =0.3154

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$ 0.5242 g 50 ml

10.484

×

mg ml

1000 mg

g




mg ml

100

100

x

mg ml

0.3154

x

mg mg × 0.3154 =10.484 x ml ml

3.01 de azucaresreductores

=

CARAMBOLA

2HON: L&*1$/( & ('+/(6*4(: 0.<= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.4849 X + 0.0071



A = 0.4849 C + 0.0071 C =0.5927

M'()*$  ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml×ρ

1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$ g 1000 mg × 50 ml g

1.020

20.4

mg ml

ENTONCES LA CANTIDAD DE AZUCARES REDUCTORES SERA .

100

mg ml mg ml

0.5927

x


100

mg × 0.5927 ml

=

20.4


mg x ml


IITA: L&*1$/( & ('+/(6*4(: 0.=?

P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.7968 X −0.0244

A = 0.7968 C −0.0244 C =0.6311

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml×ρ

1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$ g 1000 mg × 50 ml g

1.020



mg ml


mg ml

100

100

mg ml

mg mg × 0.6311=20.4 x ml ml

x =3.09 de azucaresreduct ores

D$)o( +o**&"o(

0.6311

x



C+6*&61/(* PRIMER 476 GRUPO 8)98l! 2HON IITA 0; 8 8   0< 8 8   0= 8 8   0> 8KK 8 K K 0? 8 8  K ;0 8K 8   ;< 8 8 K  ;= 8K 8  

A'. VS. C+6*&61/(*4+6 . .

<9@:  .K@  . R  

.K ABSORBANCIA .

<9@:  .@  . R  

. . . . . . . .K . . . . CONCENTRACION

JHON L&!$* 9JHON: INSTITUTO L&!$* 9INSTITUTO:



GRAFICA Nº 0=

HALLANDO CANTIDAD DE AZUCARES REDUCTORES:

NARAN2A

2HON: L&*1$/( & ('+/(6*4(: 0.<;= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.4951 X −0.0076

A = 0.4951 C −0.0076 C =0 .4490

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0506

g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

mg ml

1000 mg

g




100

10.506

mg ml

100

mg ml

0.4490

x

mg mg × 0.4490=10.506 x ml ml


IITA: L&*1$/( & ('+/(6*4(: 0.33 P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.8355 X −0.0606

A = 0.8355 C −0.0606 C =0. 4770

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 0.5 ml × 1.0506


g ml

0.5253 g

Co!+!)*$+&1! " %$ =o%$ 0.5253 g 50 ml

10.506

×

1000 mg

g

mg ml


100

10.506

mg ml

0.4770

100

mg ml

x

mg mg × 0.4770=10.506 x ml ml




PIA

2HON: L&*1$/( & ('+/(6*4(: 0.;3> P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.4951 X −0.0076

A = 0.4951 C −0.0076 C =0. 2973

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$

UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL 0.5253 g 50 ml

10.484

×


1000 mg

g

mg ml


mg ml

100

100

mg ml

0.2973

x

mg mg × 0.2973=10.484 x ml ml

x =2.84 de azucares reduct ores

IITA: L&*1$/( & ('+/(6*4(: 0.<<>

P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.8355 X −0.0606

A = 0.8355 C −0.0606 C =0.3441



M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 0.5 ml×

ρ

0.5 ml × 1.0484

g ml

0.5242 g

Co!+!)*$+&1! " %$ =o%$ 0.5242 g 50 ml

10.484

×

1000 mg

g

mg ml


mg ml

100

100

mg ml

mg mg × 0.3441=10.484 x ml ml


0.3441

x



CARAMBOLA

2HON: L&*1$/( & ('+/(6*4(: 0.<= P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.4951 X −0.0076

A = 0.4951 C −0.0076 C =0.6101

M'()*$  ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml×ρ

1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$



g 1000 mg × g 50 ml

1.020

20.4

mg ml

ENTONCES LA CANTIDAD DE AZUCARES REDUCTORES SERA .

100

100

mg × 0.6101 ml

20.4

=

mg ml

mg ml

0.6101

x

mg x ml


IITA: L&*1$/( & ('+/(6*4(: 0.=?

P$*$ 3$%%$* %$ +o!+!)*$+&1! *,/%$-$,o( ! %$ <1*,'%$ Y =0.8355 X −0.0606



A = 0.8355 C −0.0606 C =0.6452

M'()*$ . ,% P$*$ ($5* +'6!)o ;'&$% ! *$,o( 1 ml×ρ

1 ml × 1.020

g ml

1.020 g

Co!+!)*$+&1! " %$ =o%$ g 1000 mg × 50 ml g

1.020

20.4

mg ml


mg ml

100

100

mg × 0.6452 ml

20.4

=


mg ml

mg x ml

0.6452

x



COMPARANDO RESULTADOS: PORCENTA2E DE AZUCARES REDUCTORES MUESTRA

PRIMER GRUPO DATOS DATOS OBTENIDOS CORREGIDO S 2HON IITA 2HON IITA

SEGUNDO GRUPO DATOS DATOS OBTENIDOS CORREGIDO S 2HON IITA 2HON IITA

NARAN2A

8

8

8

8

8K

8

8

8

PIA

8

8K

8

8

8

8

8K

8K

CARAMBOL A

8

8

8

8K

8

8

8

8

VI.

DISCUSIONES

C$(614*(*476 & (*(/&' 1+1(l&' •

E% +o!)!&"o " $-2+$*( )o)$%( ( ")*,&!1 $/%&+$!"o % ,0)o"o DNS ')&%&-$!"o %'+o($ +o,o ()6!"$* 9 ,4,L:. E! %$ *$=+$( /'" *( %$ +'*$ /$)*1! +o!()*'&"$ /$*$ +'$!)&=+$* %o( $-2+$*( )o)$%( y ! %$ T$5%$( $3# %o( *('%)$"o( /$*$ +$"$ ,'()*$. E()o( *('%)$"o( &!"&+$! ;' %$ ,!o* +o!+!)*$+&1! " $-2+$*( )o)$%( ( !+'!)*$ ! %$ +$*$,5o%$8 %$ /&>$ )&! '!$ +o!+!)*$+&1! ,6( %$"$ y %$ ,$yo* +o!+!)*$+&1! ( /*(!)$ ! %$ !$*$!7$. E! %$ ,&(,$ )$5%$ ( /*(!)$! %$( +o!+!)*$+&o!( " %o( $-2+$*( !o *"'+)o*(. Lo( *('%)$"o( /$*$ $-2+$*( !o *"'+)o*( +o&!+&"! ! ;' % ,$yo* +o!)!&"o ( !+'!)*$ ! !$*$!7$ y % ,!o* ! %$ +$*$,5o%$8 ()o *('%)$ %1&+o8 /'()o



;' %$ ,$yo* +o!+!)*$+&1! " $-2+$*( *"'+)o*( ( !+'!)*$ ! %$ !$*$!7$ 9.: y %$ ,!o* ! %$( +$*$,5o%$ 98 $ 8: .E% ,$yo* +o!)!&"o /$*$ %$ !$*$!7$ /o(&5%,!) ( "5$ $ %$ 3&"*1%&(&( 6+&"$ " %o( /o%&($+6*&"o( o+'**&"$ "'*$!) %$ +o++&1! %$ +'$% %&5*$ o)*o( $-2+$*( ,6( (&,/%( .Lo( +'$%( +o!)&!! +$*5o3&"*$)o( +o,/%7o( ;' ( "*$"$! /o* %$ $++&1! " %$ $+&"- "% 7'o y "% +$%o* ! % +o+&,&!)o E! ()$ )$/$ ( &,/o*)$!) +o!)*o%$* %$ ),/*$)'*$8 (& ( ,6( 5$7$ ;' %$ !+($*&$8 % /o%&($+6*&"o !o ( "*$"$*6 y (& ( ,'y $%)$ o+'**&*6 %$ +$*$,%&-$*#$! " %o( $-2+$*(8 ! )o"o +$(o ( )!"*6 ,!o* +$!)&"$" " $-2+$*( . E% ! o)*o %$"o % +o!)!&"o /*o,"&o " $-2+$*( *"'+)o*( /*(!)( ! %$ /&>$8 $*#$ !)* 9  : . Po* 7,/%o +'$!"o '!$ /&>$ )&! '! +o!)!&"o ,!o* $  ( +o!(&"*$"o " 5$7$ +$%&"$" y (& /*(!)$ !)* %  y  ( " 5'!$ +$%&"$". L$( +'*$( " +$%&5*$+&1! ,o()*$*o! +o=+&!)( " +o**%$+&1! ,'y +*+$!o( $ %$ '!&"$" /$*$ (o /*/$*$*o! (o%'+&o!( /$)*o!( &!"&&"'$%( +o! %o( $-2+$*( /$*$ $(# ")*,&!$* % )&,/o " *)!+&1! " +$"$ $-2+$*8 ')&%&-$!"o %$( ,&(,$( +o!"&+&o!( ')&%&-$"$( ! %$ ,-+%$  &!y+)$!"o "o( +( /$*$ * %$ */*o"'+&5&%&"$" "% ,0)o"o. DETERMINACIN DE AZÚCARES REDUCTORES POR LATJCNICA DE MILLER DNS!. B44K6 L. M. E. R+(' R. M. A. E% DNS 96+&"o "&!&)*o($%&+#%&+o:8 ( '! *$+)&o ;' )&! %$ +$/$+&"$" " o@&"$* $ %o( $-2+$*( *"'+)o*( "$!"o *('%)$"o( +o%o*&,0)*&+o( ;' ( /'"! ,"&* +o! '!$ %o!&)'" " o!"$ " !,. Para nuestra práctica determinamos la curva de calibración con una longitud de onda de 540, y contrastando con la bibliografía decimos que el rango de longitud de onda fue la adecuada.

Lo( $-2+$*( *"'+)o*( /o(! '! *'/o +$*5o!&%o %&5*
UNIVERSIDAD NACIONAL DEL SANTA FACULTAD DE INGENIERIA EAP. INGENIERIA AGROINDUSTRIAL •

•


!os damos cuenta que el porcenta%e de &idratos de 'arbono o 'arbo#idratos en la naran%a es de (.)g de *00g de producto comestible+ pero, en el caso de glucosa es .4g de *00g de producto comestible. !otamos a#ora, que en la práctica nosotros #emos determinado -$cares /eductores en este caso de la naran%a, pero recordemos que a$cares reductores encierra grupos de glucosa, fructosa+ por ende no solo determinamos glucosa sino tambin fructosa, y este resultado se e1presó en con%unto.

"E!"2322-

U! ,0)o"o $!$%#)&+o (*6 ,6( (!(&5% +'$!)o ,$yo* ($ %$ $*&$+&1! " %$ (>$% $!$%#)&+$ y /$*$ '!$ $*&$+&1! " %$ +o!+!)*$+&1! "% $!$%&)o @ "$"$. L$ (!(&5&%&"$" 9S: " '! ,0)o"o &! "$"$ /o* %$ /!"&!) " %$ +'*$ " +$%&5*$"o. E! () +$(o )o,$!"o %$( "o( ,'()*$(

ANALISIS UNIVERSIDAD NACIONAL DEL SANTA INSTRUMENTAL DE FACULTAD DE INGENIERIA PRODUCTOS EAP. INGENIERIA AGROINDUSTRIAL AGROINDUSTRIALES D(1+' /&(l&' I )/$@+ D(1+' *+//&)4+' I )/$@+ y  .KK@  .   0.>>03 - 0.0=?3

R  0.?<   0.33 - 0.00

R  .K y  .@  .

R  0.?<

R  .

D(1+' /&(l&' II )/$@+   0.>? - 0.0<== R  0.?>;   0.=?=  0.00; R  0.??

D(1+' *+//&)4+' II )/$@+ y  .K@  . R  .KK

y  .@  . R  .K

C+8+ +'&/5(8+' &6 l(' &*$(*4+6&' Q 1&6&8+' & l+' +' )/$@+' &6 l+' (1+' /&(l&'  *+//&)4+' +'&/5(8+' Q$& 6+ ( 1(61( 4&/&6*4( &6 &l R @&/+ '4 6+' (8+' *$&61( &6 l(' &*$(*4+6&' &6*+61/(8+' Q$& l+' (1+' /&(l&' &l @/48&/ )/$@+ 14&6&6 ( 1&6&/ $6( '&6'44l4( 8(+/ @+/ l( 4&/&6*4(  *+6   &4'14/( $6( 5(/4(*476 & *+6*&61/(*476 &l (6(l41+ (&8K' l+' 8K' '&6'4l& '&/(6 l+' (1+' (6(l4(+' @+/ &l 46'141$1+ '+6 l+' Q &'1K6 /&8(/*(+'!

VII.

CONCLUSIONES


•

•

•


E! !'()*$

VIII.


CUESTIONARIO ;. "C$Kl&' '+6 l+' 8%1++' Q$& *$(l41(145(8&61& l+' ($*(/&' &'*/(l+'.

&1&/846(6 /&$*1+/&',

D&1&/846(*476 *$(6141(145( & (*(/&' /&$*1+/&'  1+1(l&' 8%1++ & L(6&  E6+6!.

E@&()! "&*(o( ,0)o"o( " +'$!)&=+$+&1! " +$*5o3&"*$)o( 5$($"o( ! %$ +$/$+&"$" *"'+)o*$ " %o( $-2+$*( ;' )&!! %&5* % *'/o +$*5o!&%o. E()o( +$*5o3&"*$)o( (o! +$/$+( " *"'+&* %,!)o( +o,o % +o5* 9C':8 % 3&**o 9F: o % yo"o 9I:. E! % +$(o (/+#=+o "% +o5*8 () ( *"'+&"o "(" C' $ C'. E! () (!)&"o8 ! % ,0)o"o " L$! y Ey!o! 9: ( 3$+ *$++&o!$* ('%<$)o +2/*&+o +o! $-2+$* *"'+)o* ! ,"&o $%+$%&!o8
E% )0*,&!o (/+)*o


;.;. R&(**4+6&' & &1&/846(*476 & $/$/(l & 4/+48&14l$/$/(l L$( /!)o($( y 3@o($( 9)$!)o $%"o($( +o,o +)o($(: ! /*(!+&$ " 6+&"o( ,&!*$%( 9/.7.8 6+&"o ('%<2*&+o: y $ ),/*$)'*$( %$"$( ('<*! /*o+(o( " "(3&"*$)$+&1! o*&&!$!"o $/$/(l 9"*&$"o( " /!)o($(: o 4/+48&14l$/$/(l 9"*&$"o( " 3@o($(:8 )$% y +o,o ( &!"&+$ ! %$ Fig.1. Fig. 1. Formación de furfurales. os monosacáridos en caliente y medio muy ácido, sufren una des#idratación que conduce a la formación de un anillo pentagonal de furfural o #idro1imetilfurfural, segn se parta de pentosas o #e1osas. os oligo6 y polisacáridos tambin sufren estas reacciones, ya que el medio ácido favorece la #idrólisis previa del enlace glicosídico. os furfurales formados se combinan fácilmente con diversos fenoles y aminas, dando reacciones coloreadas.

E()o( +o,/'()o(8 <'*<'*$% o 3&"*o@&,)&%<'*<'*$%8 *$++&o!$! +o! +o,/'()o( $*o,6)&+o( 9<!o%(8 $,&!$( +#+%&+$( o 3)*o+&+%o(: "$!"o /*o"'+)o( +o%o*$"o(. A'!;' (o! /*'5$( (/+#=+$( " ,o!o($+6*&"o(8 %o( o%&o y /o%&($+6*&"o( )$,5&0! "$! /o(&)&o8 y$ ;' ! ,"&o 6+&"o ( 3&"*o%&-$ % !%$+ %&+o(#"&+o. S "&(/o!! " "&<*!)( )0+!&+$(8 "/!"&!"o "% *$+)&o ')&%&-$"o 9!($yo " Mo%&(38 +o!  !$<)o%? !($yo " %$ $!)*o!$? !($yo " B&$%8 +o! o*+&!o%? !($yo " S%&W$!oX8 +o! *(o*+&!o%:. E! %$ /*(!) /*6+)&+$ ( %%$*6 $ +$5o %$ ")*,&!$+&1! +'$%&)$)&$ " $-2+$*( ,"&$!) %o( (&'&!)( !($yo( Mo%&(38 B&$% y S%&W$!oX 9Fig. 2:



;.;.;.- E6'(+ & M+l4': R$++&1! " <'*<'*$%  3&"*o@&,)&%<'*<'*$% +o! !$<)o% 9Fig. 2:8 "$!"o %'$* $ derivados de color prpura . E( '!$ /*'5$ !*$% " $-2+$*(.

;.;.<.E6'(+ & B4(l: R$++&1! "% <'*<'*$%  3&"*o@&,)&%<'*<'*$% +o! o*+&!o% 9 Fig. 2: ! ,"&o +o! HC%8 "$!"o %'$* $ derivados de color a$ul . E( '!$ /*'5$ (/+#=+$ " /!)o($(.

;.;.3.- E6'(+ & S&l4(6+: R$++&1! "% <'*<'*$%  3&"*o@&,)&% <'*<'*$% +o! *(o*+&!o% 9 Fig. 2:8 "$!"o %'$* $ "*&$"o( " color rosa6 ro%o. E( '!$ /*'5$ (/+#=+$ " 3@o($(. L$( +)o($( ! ,"&o 6+&"o ( "(3&"*$)$! ,6( *6/&"$,!) ;' %$( $%"o($(8 /o* %o ;' $,5o( *'/o( /'"! "&<*!+&$*( ! <'!+&1! "% )&,/o ;' )$*"$ ! $/$*+* % /*o"'+)o +o%o*$"o.



Fig. 2. Reacciones coloreadas de los furfurales con 76naftol 8prueba de 9olis#:, resorcinol 8prueba de "elli;ano<: y orcinol 8prueba de 3ial:.

;.<. I&614*(*476 & (*(/&' /&$*1+/&' @+/ &l &6'(+ & F&l46)

Lo( +$*5o3&"*$)o( ;' /*(!)$! *'/o( $%"3#"&o( %&5*( o !
Fig. 3. Reacción de oxidación de azúcares por iones cúpricos



N&)(145+: Al&4+' (/+8K14*+'  *&1+6(' P+'4145+ Al&+' (l4K14*+'  -4/+4(l&4+'

U! 7,/%o )#/&+o " $-2+$*( *"'+)o*( (*#$! %$ glucosa y %$ maltosa 8 y " $-2+$*( !o *"'+)o*(8 % "&($+6*&"o sacarosa 9=D <*'+)o<'*$!o(&%9:D%'+o/&*$!1(&"o:. L$( +)o($(8 ;' !o (o! $-2+$*( *"'+)o*(8 ('<*!8 (&! ,5$*o8 *$++&o!( " o@&"$+&1! )#/&+$( " %o( $%"3#"o(? /$*$ %%o !+(&)$! '! ,"&o 56(&+o8 y$ ;' $ $%o*( $%)o( " /H ( /*o"'+ '!$ &(o,*&-$+&1! $ %$ +o**(/o!"&!) $%"o($. H$y "&<*!)( ,0)o"o( " ")*,&!$+&1! " $-2+$*( *"'+)o*( 9F3%&!8 B!"&+)8 B$*
E% +$(o "% $%,&"1! ( "&()&!)o (& 5&! (# /o( +$*5o!o( $!o,0*&+o( %&5*( +$/$+( " o@&"$*(8 3$y ,'y /o+o( ! +o,/$*$+&1! $ %$ +$!)&"$" " '!&"$"( " ,o!o($+6*&"o( ;' +o,/o!! % $%,&"1!. ( "+&*8 (o%o %o( " %o( @)*,o( " %$ %$*$ +$"!$ ;' +o,/o! $% $%,&"1!8 /'" o@&"$*( y +$%+'%$ ;' '!$ +$"!$ " $%,&"1! 9;' $ (' - )&! *$,&=+$+&o!(: )&! ,6( "  *(&"'o( " ,o!o($+6*&"o(8 !)o!+(8 (& 5&! % $%,&"1! /'" o@&"$*(8 (' /o"* *"'+)o* ( /*6+)&+$,!) !'%o8 "5&"o $% ,'y 5$7o *!"&,&!)o ;' /o( A+%$*$+&o!( % +$*5o!o $!o,0*&+o ( % +$*5o!o !2,*o  ! %$( $%"o($(8 ( % +$*5o!o ;' )&! %o( ('()&)'y!)( 9OHy H: $/)o( $ o@&"$*( %$ ($+$*o($ ()6


3. "#$% $6*476 *$8@l& &l l(6*+ &6 l( 8&4*476 & ('+/(6*4(,

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Determinacion de Azucares Reductores Por El Metodo Del DNS

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