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Práctica Nro. 3 Determinación de Glicemia y Glucosuria I . -
I NTRODUCCI ÓN LA GLI CEMI MI A Es una medi da de concent r aci ón de gl ucosa en elpl ásma.
ayuna, l os ni vel es nor mal es de gl ucosa son ent r e 70mg/dl 120mg mg/dl .Cuando l a gl i cemi a esma masbaj a de l os70mg/dl habl a de hi pogl i cemi a, per o cuando l a gl i cemi a super a 120mg mg/dlhabl amo mosdehi per gl i cemi a
i cemi mi a se usa comú La gl múnme ment e par a det ect ar di abe
enf er medad en l a cualse obser van ni vel es al t os de gl ucos hi per gl i c emi a) ( . Est os, con el t i emp mpo, pueden causar ser
Master your semester with Scribd probl ema masd esal ud .La diabetes esunaenf er medad me t aból Read Free Foron 30this Days Sign up to vote title & The New York Times Useful Not useful ns ul i na,ho r esul t adodel adefici enci adel ai r mona encar gada Special offer for students: Only $4.99/month.
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permi mi t i rl aent r adadelazúcaral ascél ul as.
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GLUCOSURI A
Esl aPr esenci aanormal ment eal t adegl ucosaen l aori na,com
porej empl oen l adi abet esmel l i t as,seut i l i zacomoi ndi cadord l osni vel esdegl i cemi aconval oresdegl ucosaensangresobr e1 mg/dlaproxi madament eyf unci ón r enalnor mal ,elexceso azúcarseel i mi naporl aori na,apareci endogl ucosuri a. You're Reading a Preview
Paramedi rgl ucosuri a seut i l i zan t i r as r eact i vas l l amadas K Unlock full access with a free trial.
Di aburTes t5000.
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Una per sona nor malno debe cont energl ucosaen su or i na.
gl ucosur i a sepr esent a usual ment e en di abet esmalcont r ol ad
Lagl ucosae mpi eza aaparecerenl aori nacuandol agl i cemi aest Master your semester with Scribd Read Free Foron 30this Days Sign up to vote title porTimes enci made160180mg/dL;aest e n i v e l s e l e d e n o mi n a “ di n & The New York Useful Not useful Special offer for students: Only $4.99/month.
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r enal ” para gl ucosa.Al gunas per sonas pueden t ener eldi n
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Losmét odosparaconocerelcont r olmet aból i coen paci ent esco di abet esmel l i t us( DM)puedensercl asi ficadoscomosepr esent cont i nuaci ón: 1.Aquél l os que bri ndan cr i t er i oi nmedi at o, ent r el os que
encuent r an l agl i cemi a( seaenayunas,pospr andi al ,oell l am doperfilgl i cémi co )yl a gl ucos ri a. You're Reading au Preview 2.
full access with a free trial. Losqueeval úanUnlock elc ont r ola l argopl azo,r el aci onadoscon
Download Free Trial det er mi naci ón d e l a gWith l i cos i l aci ón no enzi mát i ca de
pr ot eí nas,l asquemi denest adoshi per gl i cémi cosocur ri dos dí asant esen elcasodel ahemogl obi nagl i cosi l ada,y15d
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Ez c ur r a E. I gl i cosi l ada. 14 ( nt r oducci ón de
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det ermi naci ón;l ossegundosusan elsul f at odecobr e( r eact i vod
Benedi ct )comoagent ereduct oryporl ot ant o,r eacci onan ant el
gl ucosa,monosacár i dos,medi cament oscomoeláci donal i dí xi eláci dopar ami nosal i cí l i co,eláci doascór bi co,l a peni ci l ami n
l ossul f ami dados,ent r eot r os,noobst ant eest asl i mi t aci ones,est úl t i moeselmásempl eadoennuest r opaí s. 57 Además,en l a gl ucosuri ai nt ervi enen ot r os f act or es como f unci onesr enalyvesi calyl aedad delpaci ent eyen est et r ab
nospr opusi mosanal i zarl ai nfluenci adeel l osen l amodi ficaci de l a gl ucosur i a,con elobj et i vo de mej orar l a cal i dad de i nt er pret aci ón de es t e proceder di agnóst i co, l o que
You're i mpr esci ndi bl e para el eReading var l aa Preview cal i dad de vi da del paci en Unlock full access with a free trial.
di abét i co, pues obt ener un cont r ol met aból i co est r i ct o, necesari oparael l o.Download With Free Trial
I I . - COMPETENCI AS
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Espect r of ot ómet r o
You're Reading a Preview
BañoMarí a
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Pi cet a
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Cubet as
Al godón
Aguj avenoj ect
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I V. - PROCEDI MI ENTO Tomarunamuest r adesangr e( apr ox.3mL) ,cent ri f ugara3, 0 r pm por10mi nut os.Separ arelsuer o.
Det er mi naci óndegl ucosasanguí nea: Col ocarent r est ubosdeensayodeacuer doalsi gui ent eesquem
REACTI VO /TUBO
BLANCO
ESTÁNDAR
MUESTR
Est ándar Gl ucosa ( 100mg/dL)
-
Muest r a( suer o)
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20ul
20ul
React i vodet r abaj o You're Reading 2mla Preview
2ml
2ml
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Mez cl arei ncubarenbañoMarí aa37°C por10mi nut os Leer a 505nm, l l evando a cer o elaparat o con elbl anco reactivo.
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Suer oopl asma :
0701–1. 10g/L
Sangr eTot al
:
0. 60–1. 00g/L
LCR
:
0. 40–0. 74g/L
Determi naci ón de gl ucosa en ori na por el método BENEDI CT Col ocaren un t ubo de ensayo8 got asde ori na pat ol ógi cay ot r ot ubo 8 got as de or i na de su compañer o( no pat ol ógi
agr egara ambos t ubos 2 mL.de r eact i vode Benedi ct ,l l evar hervi rdur ant e3mi nut os,enf ri arei nt erpr et arl osr esul t ados.
I NTERPRETACI ÓN: Col or Azul Axzuxlver doso Ver de Ver depar duzco Amar i l l o Roj ol adr i l l o
I nterpretaci ón Negat i vo Vest i gi os Unlock full access with a freeP trial. osi t i vo+ Posi t i vo++ Download With Free Trial Posi t i vo+++ Posi t i vo++++ You're Reading a Preview
Nor mal ment e en l a or i na no hay azúcar es,su presenci a
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el$4.99/month. i ncr ement odel agl ucosaen sangr esi n embargo,hayal gun Special offer for students: Only
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1. - Segúnl aúl ti macl asi ficaci ón del aOMS,cuantosti posde di abetesexi st en.
t e d St at e s Nat i o n En l a cl asi ficaci ón i nt r oduci da por l a Uni Di abet es Dat a Gr oup ( NDDG)e n 19791 y t omada con al gu
modi ficaci onesporl aOr gani zaci ónMundi aldel aSal ud( OMS)
4 19802 yl uegor efinadaen19853 y1994, sei ncl uyen5cat egorí
La di abet es mel l i t us i nsul i nodependi ent e ( DMI D) , di abet mel l i t us no i nsul i nodependi ent e ( DMNI D) , di abet es mel l i r el aci onadacon mal anut ri ci ón,di abet esgest aci onal( DG)yot t i posdedi abet esasoci adosaci ert ascondi ci onesysí ndr omes. 2pri nci pal est i posdedi abet esson et i ol ógi cament edi f erent es. l aact ual i dadset r at adesust i t ui rl ost ér mi osDMI D yDMNI D di abet est i po1yt i p o2( s uger i do desde1980) ,2 quer epresen You're Reading as Preview l os mecani smos p at og én i cos , aa untrial. que est a sust i t uci ón Unlock full access with free sol uci ona l a probl emát i ca de una cl asi ficaci ón basada en Download With Free Trial
t r at ami ent o.
Ladi abet est i po1yl at i po2sepuedendi st i ngui rporunaseri ed car act er í st i cas como son:edad ( t empr ana vs,t ar dí a)ymodo Master your semester with Scribd Read Free Foron 30this Days Sign up to vote title present aci ón ( agudo vs,l ent o) ,est ado ponder al( baj o pe so & The New York Times Useful Not useful Cancel anytime.
Special offer for students: Only $4.99/month. sob r epeso) ,epi sodi osdecet osi s,hi st ori af ami l i ar ,asoci aci ón co
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2. - Descri bal osti posdei nsul i narecombi nantes. 2. 1. -Segúnelpuntodevistadesuori gen:
I nsul i nahumana:Esl aqueseut i l i za.Puedeser : o
Semi si nt ét i ca:Apart i rdei nsul i naporci na,semodi ficaba am.30del acadenaB ( al ani naport r eoni na)yseconver t eni nsul i nahumana.
o
Bi osi nt ét i ca:Sí nt esi spori ngeni erí asi nt ét i ca.Medi ant eu pl ásmi dosei nt r oduceenunabact eri a( E. col i )l a i nf ormaci ónnecesari apar apr oduci ri nsul i naopr oi nsul i Esl aqueseusahoydí a.
I nsul i naani mal :Sedi f er enci adel ai nsul i nahumanaenel
cont eni dodeami noáci dos.Ti enecapaci dadi nmunogéni caypor t ant odepr oduci rr ea cci on esal ér i cas. You're Reading ag Preview o
I nsul i nabobi n a:Sfull ed i f er e ncai a en 3ami noáci dos. Unlock access with free trial.
o
I nsul i naporci na:Sedi f er enci aenelami noáci do30del a Download With Free Trial
cadenaB ( esal ani na,yenl ahumanaest r eoni na) .
2. 2. segúnelti ewith mpode actuaci ón: Master your semester Scribd Read Free Foron 30this Days Sign up to vote title Lai nsul i nacomot alt i eneunavi dame di ade35mi nut os ques & The New York Times Useful Not useful Cancel anytime.
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I nsul i nadeacci óni nt er medi a:i ni ci odeacci ónde30mi nut osa hor as,pi co en 36 hor as y una dur aci ón de 1824 hor as. i nyect an 2 vecesaldí a,ant esdeldesayuno yl a cena.Tenem dost i posdei nsul i nasi nt ermedi as:
•
I nsul i naNPH.
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I nsul i nal ent a.
I nsul i nadeacci ón l ar ga oul t r al ent a:i ni ci odeacci ón al as hor as,pi coen 8-14 hor asy dur aci ón deacci ón de2436 hor Sepone1vezaldí a.
Comol osdat osdelFarr er asson di f er ent es,aquíval at abl a.
3. 3. -Segúnl aví adeadmi ni st raci ón:
Subcutánea.
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3. - Comoseproducel ai nsul i narecombi nante.
Medi ant el ai ngeni erí agenét i caquet i eneungranpot enci Master your semester with Scribd Read Free Foron 30this Days Sign up to vote title Porej empl o,elgen par al ai nsul i na,queporl ogener alsól & The New York Times Useful Not useful Cancel anytime.
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VI . BI BLI OGRAFI A 1.DEVLI N, T. M. Bi oquí mi ca,Li br odet ext oconapl i caci ones Cl í ni cas.3daedi ci ón.1999Ed.Rever t é.Bar cel ona 2.GONZALESDEB.yOt r os.Bi oquí mi caCl í ni ca. 2da Edi ci ón. 2002.MacGr awHi l lI nt er amer i cana.Madr i d 3.LAGUNAJOSE bi oquí mi cadel aguna5t aedi ci ón2002 . Méxi co 4.LEHNI NGER,A.Bi oquí mi ca.1998. Ed. Omega.Bar cel ona.
5.LOZANO y Col .Bi oquí mi cayBi ol ogí aMol ecul arparaCi enci del aSal ud.2da. Edi c.2001.Edi t .McGr awHi l l– I nt er amer i cana.España. 6.MONTGOMERY,C Bi oquí mi ca.CasosyText o. 6t a Edi ci ón. 1999. Madri d
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7.MURRAYy ColUnlock B i oquí mi cadeHar per . 15va.Edi ci ón full access with a free trial. 2001Ed.ManualModer no.Méxi co.
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8.STRYER ,L. Bi oquí mi ca. 4t aEdi ci ón.1999Edi t .Rever t e. España. 9.VI LLAVI CENCI O,M.Bi oquí mi ca.1er aEdi ci ón1999UNMSM
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