LAPORAN TETAP PRAKTIKUM BIOKIMIA II
UJI KUANTITATIF PROTEIN Nama
: Lusiana Setiawati
NIM
: 061011813008
Ke!"m#"$
: 1 % Satu&
An''"ta
:1( )ia* Pe+mata Sa+i ( Nu+u! Mawa,,a* 3( Met- T++ian.( Reni Ma+/e!a ( Reni O$taia 2ana+ Na*"+ 6( 4i!,an Ti$t" R
)"sen Pem5im5in'
: )esi S(P,( M(T( )ia* Ka+ti$a Sa+i S(P,( M(Si(
PROGRAM STUDI PENDIDIKAN KIMIA FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS SRIWIJAYA 2016
LAPORAN TETAP PRAKTIKUM BIOKIMIA II I. II. III. IV.
PERCOBAANKE :4(E!"#$ TANGGAL PRAKTIKUM : 1 2 F%&'"') 2 016 JUDUL PRAKTIKUM : U*) K"+#)#"#), P '-#%)+ TUJUAN PRAKTIKUM : Menentu$an $a,a+ #+"tein -an' te+,a#at ,a!am sam#e! ,en'an met",e tit+asi 7"+m"!(
V.
DASAR TEORI P+"tein ,itemu$an ,i ,a!am semua se! ,an semua 5a'ian se! -an' amat
5e+a+iasi -aitu te+,a#at +atusan enis -an' 5e+5e,a ,a#at ,itemu$an ,a!am satu se!( P+"tein 5e+#e+an #entin' ,a!am st+u$tu+ ,an 7un'si semua se! ma$*!u$ *i,u# ,an i+us( Pe+anan #entin' #+"tein anta+a !ain untu$ mem5entu$ a+in'an "t"t /at #en'atu+ se+ta se5a'ai a,a n'an ma$anan ,an ene+'i ,a!am tu5u*( P+"tein me+u#a$an sa!a* satu ,a+i 5i"m"!e$u! +a$sasa se!ain #"!isa$a+i,a !i#i, ,an #"!inu$!e"ti,a -an' me+u#a$an #en-usun utama ma$*!u$ *i,u# -an' u'a me+u#a$an #"!ime+ ,a+i m"n"me+9m"n"me+ asam amin" -an' ,i*u5un'$an satu sama !ain ,en'an i$atan #e#ti,a( P+"tein 5e+asa! ,a+i 5a*asa unani #+"t"s -an' 5e+a+ti ;-an' #a!in' utama<( P+"tein me+u#a$an sen-awa "+'ani$ $"m#!e$s 5e+5"5"t m"!e$u! tin''i -an' me+u#a$an #"!ime+ ,a+i m"n"me+9m"n"me+ asam amin" -an' ,i*u5un'$an satu sama !ain ,en'an i$atan #e#ti,a( M"!e$u! #+"tein men'an,un' $"m#"sisi +ata9+ata unsu+ $imia -aitu $a+5"n 0= *i,+"'en >= "$si'en 3= nit+"'en 6= ,an $a,an' $a!a su!7u+ 093= se+ta 7"s7"+ 093=( P+"tein ,a#at ti,a$ sta5i! te+*a,a# 5e5e+a#a 7a$t"+ -aitu #? +a,iasi su*u me,ium #e!a+ut "+'ani$ ,an ,ete+'en( P+"tein ,a#at ,i#e+"!e* ,a+i ma$anan -an' 5e+asa! ,a+i *ewan atau tum5u*an( P+"tein -an' 5e+asa! ,a+i *ewan ,ise5ut #+"tein *ewani se,an'$an -an' 5e+asa! ,a+i tum5u*an ,ise5ut #+"tein na5ati( 2e5e+a#a ma$anan sum5e+ #+"tein a,a!a* ,a'in' te!u+ susu i$an 5e+as $aan' $e,e!ai 'an,um a'un' ,an 5ua*95ua*an( Tum5u*an mem5entu$ #+"tein ,a+i @O ?O ,an sen-awa nit+"'en( ?ewan -an' ma$an tum5u*an men'u5a* #+"tein na5ati mena,i #+"tein *ewani( )isam#in' ,i'una$an untu$ #em5entu$an se!9se! tu5u* #+"tein u'a ,a#at ,i'una$an se5a'ai sum5e+ ene+'i a#a5i!a tu5u* $e$u+an'an $a+5"*i,+at ,an !ema$( San'at !ua+ 5iasa #u!a 5a*wa semua #+"tein ,i ,a!am semua ma$*!u$ tan#a meman,an' 7un'si ,an a$tiitas 5i"!"'in-a ,i5an'un "!e* susunan ,asa+ -an' sama -aitu 0 asam amin" 5a$u -an' m"!e$u!n-a sen,i+i ti,a$ mem#un-ai a$tiitas 5i"!"'i( Sea+a u$u# se,e+*ana #+"tein 5e+5e,a satu sama !ain $a+ena masin'9masin' mem#un-ai deret unit asam amin" sen,i+i9sen,i+i( Asam amin"
me+u#a$an a5a, st+u$tu+ #+"tein $a+ena m"!e$u!9m"!e$u! ini ,a#at ,isusun ,a!am um!a* ,e+et -an' *am#i+ ti,a$ te+5atas untu$ mem5uat 5e+5a'ai #+"tein ,a!am um!a* -an' *am#i+ ti,a$ te+5atas #u!a %Le*nin'e+ 18& )a!am $e*i,u#an #+"tein meme'an' #e+anan -an' #entin' #u!a( P+"ses $imia ,a!am tu5u* ,a#at 5e+!an'sun' ,en'an 5ai$ $a+ena a,an-a en/im suatu #+"tein -an' 5e+7un'si se5a'ai 5i"$ata!is( )i sam#in' itu *em"'!"5in ,a!am 5uti+95uti+ ,a+a* me+a* atau e+it+"sit -an' 5e+7un'si se5a'ai #en'an'$ut "$si'en ,a+i #a+u9#a+u $e se!u+u* 5a'ian tu5u* a,a!a* sa!a* satu enis #+"tein( )emi$ian #u!a /at9/at -an' 5e+#e+an untu$ me!awan 5a$te+i #en-a$it atau -an' ,ise5ut anti'en u'a suatu #+"tein %P"e,ia,i 00&( VI.
ALAT DAN BAAN A/"# 1( E+!enme-e+ ( Stati7 3( 2u+et .( Pi#et tetes ( Be!as u$u+ 6( M"+ta! >( @awan #"+se!en 8( Ke+tas sa+in' B""+ 1( La+utan sam#e! % "$!at tem#e ,an nasi& ( NaO? 01 M 3( ACua,est .( K9O$sa!at enu* ( F"+ma!,e*i, .0=
VII.
PROSEDUR PERCOBAAN G"&"'
K%#%'"+"+ ?a!us$an Sam#e!
Tim5an' Sam#e! se5an-a$ '+am
La+ut$an sam#e! ,en'an 100 m! aCua,es
Sa+in' !a+utan sam#e!
Men'am5i! 10 mL !a+utan sam#e! $e ,a!am e+!enme-e+ 100 mL $emu,ian menam5a*$an 0 mL aCua,est ,an 0. mL K9"$sa!at enu* se+ta 1 mL in,i$at"+ PP ,an men,iam$ann-a se!ama menit(
Mentit+asi sam#e ,en'an !a+utan NaO? 01 N sam#ai !a+utan 5e+wa+na #in$(
Kemu,ian menam5a*a$an mL !a+utan 7"+ma!,e*i, .0 = !a!u me!anut$an menit+asi sam#ai !a+utan 5e+wa+na #in$( Menatat "!ume NaO? -an' te+#a$ai(
VIII. ASIL PENGAMATAN L"'#"+ B/"+T)#'"3
V-/
P%+"&""
V-/
)
N"O
+
N"O
01mL 01 mL
1 2
G"&"'
F-'"/%) 2enin' 01mL 2enin' 0 mL
L"'#"+ S"!%/ S"!%/ 1
V-N / "O 0> mL 08 mL
T%!%
1
0> mL 08 mL
1
03 mL 03 mL
C-/"#
N"3)
G"&"'
I5.
PERSAMAAN REAKSI
5.
PERITUNGAN
%Protein=
)i$eta*ui:
Volume NaOH × N NaOH × 14,008 × 100 Berat sampel × 10 mL 2e+at sam#e! : '+am M NaO? : 01 M Volume NaOH = Volume Titrasi I + Volume Titrasi 2 2
Tempe=
0,7 + 0,8
Coklat =
2
=
0,7 + 0,8 2
0,75 mL
=
0,75 mL
+
Nasi = 0,3 03, = 0,3 mL 2
)itan-a: = P+"teinD Pen-e!esaian: %ProteinTempe=
%ProteinCoklat =
0,75 × 0,1 × 14,008 5 × 10 mL
0,75 × 0,1 × 14,008 5 × 10 mL
× 100 = 2,1
× 100 =2,1
%Protein Nasi= 0,3 × 0,1 × 14,008 × 100 = 0,8 5 × 10 mL
5I.
PEMBAASAN Pe+"5aan $a!i ini -aitu tentan' #+"tein( P+"tein me+u#a$an suatu sen-awa
#"!ime+ -an' te+5entu$ ,a+i m"n"me+9m"n"me+ asam amin" -an' ,i*u5un'$an "!e* i$atan #e#ti,a anta+a asam amin" satu ,en'an asam amin" !ainn-a( P+"tein 5e+7un'si mem5entu$ a+in'an atau 5a'ian tu5u* !ain untu$ #e+tum5u*an se5a'ai en/im %me+u#a$an $ata!isat"+& t+ans#"+t m"!e$u! ,i ,a!am ,a+a* ,an se! se5a'ai *"+m"ne "nt"*n-a *"+m"ne insu!in se5a'ai #em5entu$ anti5",- m"!e$u! -an' mem5antu $"nt+a$si "t"t $eseim5an'an ai+an t+ansmisi sa+a7( )a!am #e+"5aan ini !a+utan sam#e! ,i5uat ,en'an me!a+utan '+am sam#e! $e,a!am 100 mL aCua ,es a,a #e+!a$uan -an' ,i5e+i$an #a,a sam#e! !a+utan tem#e !a+utan "$!at ,an !a+utan nasi( Se#e+ti #enam5a*an ai+ #a,a $eti'a sam#e! ini 5e+tuuan untu$ men'*i,+"!isis #+"tein -an' te+,a#at #a,a sam#e! tem#e "$!at ,an nasi mena,i asam9asam amin"( Penam5a*an ,inat+ium "$sa!at enu* 5e+tuuan untu$ mem5!"$a,e 'u'us amina
%9N?& #a,a asam amin" se*in''a *an-a te+,a#at 'u'us
$a+5"$si! %9@OO?& #a,a uun' +antai -an' a$an 5e+ea$si ,en'an NaO? sam#ai !autan te+se5ut 5e+u5a* mena,i 5e+wa+na me+a* mu,a( Penam5a*an In,i$at"+ PP 5e+tuuan untu$ se5a'ai 5atas #enan,a 5e+a$*i+n-a tit+asi( Pe+!a$uan se!anutn-a semua !a+utan
,itit+asi ,en'an !a+utan NaO? 01 N -an' 5e+tuuan untu$ menet+a!$an 'u'us $a+5"$si! -an' te+,a#at #a,a asam amin"( Saat mentit+asi $eti'a sam#e! !a+utan ,a+i !a+utan tem#e "$!at ,an nasi wa+na !a+utan 5e+u5a* mena,i #in$( Pa,a #e+!a$uan se!anutn-a #enam5a*an ,en'an 7"+ma!,e*i, -an' 5e+tuuan untu$ men'uat$an si7at asam ,a+i asam amin" *a! ini ,itan,ai ,en'an *i!an'n-a wa+na #in$ #a,a !a+utan se!anutn-a !a+utan ,itit+asi $em5a!i sam#ai !a+utan 5e+u5a* mena,i #in$ $em5a!i( Pe+u5a*an wa+na !a+utan mena,i #in$ ,ise5a5$an $a+ena si7at ,a+i in,i$at"+ ## -an' a$an 5e+wa+na #in$ #a,a !a+utan 5asa %se#e+ti NaO?&( Pa,a 5e+"5aan men''una$an !a+utan 5!an$" ,i'una$an aCua,es -an' ,itam5a*$an ,en'an )inat+ium "$sa!at enu* ,an in,i$at"+ ## wa+na !a+utan !an'sun' 5e+u5a* #in$ ini menan,a$an 5a*wa !a+utan 5!an$" ini ti,a$ men'an,un' #+"tein( A,a#un tuuan ,a+i !a+utan 5!an$" ini -aitu untu$ men'eta*ui um!a* m! NaO? -an' 5e+ea$si ,en'an /at9/at $imia -an' ,i'una$an ,a!am ana!isis -aitu )inat+ium "$sa!at enu* 7"+ma!,e*i, ,an ai+( )a+i *asi! tit+asi ,i#e+"!e* "!ume NaO? -an' ,i'una$an untu$ !a+utan tem#e "$!at ,an nasi a,a!a* 0> mL 0> mL ,an 03 mL( Pe+5e,aan "!ume te+se5ut ,ise5a5$an $a+ena $a,a+ #+"tein ,a+i tia#9ti# sam#e! 5e+5e,a( 2e+,asa+$an #e+*itun'an ma$a ,a#at ,i$eta*ui $a,a+ #+"tein ,a+i sam#e! -an' ,i'una$an -aitu untu$ !a+utan sam#e! tem#e 1= "$!at 1= ,an nasi 08=( 5II.
KESIMPULAN 1( P+insi# tit+asi 7"+ m"! a,a!a* met",e ana!isis a+a #enentuan #+"tein ,en'an a+a
tit+asi 7"+m"! untu$ men'*i,+"!isis #+"tein ,a!am sam#e!( ( Sema$in 5an-a$ !a+utan NaO? -an' ,i'una$an ma$a sema$in tin''i $a,a+ #+"teinn-a 5e'itu#un se5a!i$n-a( 3( Sam#e! -an' memi!i$i $a,a+ #+"tein te+tin''i a,a!a* tem#e ,an "$!at se,an'$an -an' memi!i$i $a,a+ #+"tein +en,a* a,a!a* nasi( .( Ka,a+ #+"tein -an' ,i#e+"!e* me!a!ui #e+"5aan untu$ tem#e a,a!a* 1= "$!at 1= ,an nasi 08=(
5III. DAFTAR PUSTAKA
Fau/ia* N( %01. Ju!i&( Analisa Protein( )i#eti$ Fe5+ua+i 0 016 ,a+i *tt#:nu+u!9 797#$11(we5(unai+(a(i,a+ti$e!,etai!910>>69Umum9Ana!isa =0P+"tein(*tm!( Le*nin'e+ A( L( %18&(Dasar - Dasar Biokimia. Ja$a+ta: E+!an''a( P"e,ia,i A( %1.&( Dasar - Dasar Biokimia . Ja$a+ta: Unie+sitas In,"nesia(
