ESCUELA SUPERIOR POLITÉCNICA DE CHIMBORAZO FACULT FACULTAD DE RECURSOS RECURSO S NATURALES FORMATO DE LABORATORIO DE BIOQUÍMICA NOMBRE: JHONNY PAREDES
CODIGO: 1966
ESCUELA: ING. AGRONÓMICA
SEMESTRE: CUARTO
MATERIA: BIOQUÍMICA I PROFESOR(A):DRA:LIVIA ORDOÑEZ NOTA INFORME…………….. INFORME…… ……….. NOTA DEFENSA……………. DEFENSA… …………. NOTA TOTAL………………
TEMA: IDENTIFICACION IDENTIFICACIO N DE AZUCARES REDUCTORES EN ESTRACTO NATURAL
OBJETIVO: I!"#$%$&'( ')*&'(!+ (!*,(!+ !" !-#(', "'#*(' ' #('/0+ ! (!'&&$,"!+ *23$&'+.
MARCO TEÓRICO
A. AZCAR REDUCTOR.
L,+ A)4&'(!+ (!*,(!+ +," '*!,+ ')4&'(!+ *! 5,+!!" +* (*5, &'(7,"$, $"#',8 *!
'
#('/0+
!
3$+3,
5*!!"
(!'&&$,"'(
&, "
,#('+
3,0&*'+.
L,+ ')4&'(!+ (!*,(!+ 5(,/,&'" ' '#!('&$" ! '+ 5(,#!2"'+ 3!$'"#! ' (!'&&$" ! *&,+$'&$" ", !")$3;#$&' !")$3;#$&' #'37$0" !",3$"'' (!'&&$" ! $&'&$". E+#' (!'&&$" +! 5(,*&! !" /'($'+ !#'5'+: '+ $"$&$'!+ $"$&$'!+ +," (!/!(+$ (!/!(+$7!+ 7!+ +! &,35! &,35!#'" #'" !" #$!35,+ (!'#$/'3!"#! &,(#,+8 3$!"#('+ *! '+ 5,+#!($,(!+ #('"+&*((!" 3;+ !"#'3!"#! +," $((!/!(+$7!+. S! 5,+#*' *! #'"#, '+ !#'5'+ $"$&$'!+ &,3, '+ %$"'!+ ! ' *&,+$'&$" !+#;" $35 $3 5$& $&' ''+ '+ !" , ,++ 5( 5(,& ,&!+ !+,+ ,+ ! !" !"/! /!
&("$&'+
!
'
$'7!#!+.
S! $&! *! *" ')4&'( !+ (!*,( &*'", 5*!! ,-$'(+!. P,( !?H>O8 (!'$/, ! @!=$" , (!'$/, ! T,!"+8 +! #('"+%,(3' ! '!=2,
!" ;&$, &'(7,-2$&,. E" !+#' (!'&&$" ' *&,+' +! ,-$' '' &,3, (!*,( (!*&! ' (!'$/, ''$,. L,+ ')4&'(!+ %,+%'#,8 *! +," ')4&'(!+ (!*,(!+ ! ('" $35,(#'"&$' !" ! $"#!($,( &!*'(8 5,+!!" 3',( &'5'&$' *&,+$'"#! *! ' *&,+' '' +* 3',( 5(,5,(&$" ! %,(3' &'(7,"2$&' '7$!(#'. MATERIALES ! REACTIVOS
A. MATERIALES:
A$#',( ! /$($,. P$5!#'+. P'5! %$#(,. R!/!(7!(,. G('$'+ &," #*7, ! !"+',+. V'+, ! 5(!&$5$#'&$" 5'(' 7', 3'(2'. A)4&'(8 !&=!8 "'('"<'8 (!3,'&='8 */'8 5'5''8 )'"'=,($'8 5$'. B.REACTIVOS:
A*'. R!'$/, ! B!"!$. R!'$/, ! %!=!$" A B. PROCEDIMIENTO:
1. P(!5'('( ! !-#(', !" *" #*7, ! !"+', 5(!/$'3!"#! (,#*',8 &,,&'( *" 5,&, ! 3*!+#(' 5(,&!!( ' (!'$)'( ! 7', 3'(2'.
>. I!"#$%$&'&$" R!'$/, ! %!=!$" C,,*! *" 5,&, ! !-#(', *!, !
RESULTADOS:
MUESTRA
REACTIVO DE BENEDICT
OBSERVACIONES
R!3,'&='
REACTIVO DE FEHELING N!'#$/,
N!'#$/,
E+ *" ')4&'( ", (!*,(
P$'
P,+$#$/,
P,+$#$/,
E+ *" ')4&'( (!*,(
U/'
P,+$#$/'
P,+$#$/'
E+ *" ')4&'( (!*,(
A)4&'(
N!'#$/'
N!'#$/'
N'('"<'
P,+$#$/'
P,+$#$/'
Z'"'=,($'
P,+$#$/'
P,+$#$/'
L!&=!
P,+$#$/'
P,+$#$/'
P'5''
P,+$#$/'
P,+$#$/'
E+ *" ')4&'( ", (!*,( E+ *" ')4&'( (!*,( E+ *" ')4&'( (!*,( E+ *" ')4&'( (!*,( E+ *" ')4&'( (!*,(
CUESTIONARIO.
") E#$%&'&% " *%+," - /%,01 %-,$21% 3 $,"4-1 # -&$ 5, # %-,$21%.
O&*5'", ,+ 5,+$&$,"!+ ,+ $+3!(,+ !",3$"',+ '",3!(,+ '%' E" 5,+$&$" #('"+ 7!#' E" 5,+$&$" &$+ S! $&! *! !+ (!*,( ' ,+ ')*&'(!+ *! 5,+!!" +* (*5, &'(7,"$, $"#',8 ' #('/0+ ! 3$+3, 5*!!" (!'&&$,"'( , +!( ,-$',+ &," ,#('+ !+5!&$!+ 7 C," # ,4-"+421 - " 0%,'" - F6&4/ 3 B4-&$2. .
FUNDAMENTO PRUEBA DE BENEDICT
L' (!'&&$" , 5(*!7' ! B!"!$ $!"#$%$&' ')4&'(!+ (!*,(!+ '*!,+ *! #$!"!" +* OH '",30($&, $7(!8 &,3, ' ',+'8 ' *&,+'8 ' 3'#,+'8 &!,7$,+'. E" +,*&$,"!+ '&'$"'+8 5*!!" (!*&$( ! C*> *! #$!"! &,,( ')* ' C*8 *! 5(!&$5$#' ! ' +,*&$" '&'$"' &,3, C*>O ! &,,( (,<,"'('"<'. L' &,,('&$" !5!"!(; ! ' &,"&!"#('&$" !
,-$,
!
&,7(!
!+#'
'
+*
/!)
!
'
(!*&&$"
!
&,7(!.
E %*"'3!"#, ! !+#' (!'&&$" ('$&' !" *! !" *" 3!$, '&'$",8 ! $," &45($&, ,#,(', 5,( ! +*%'#, &45($&, !+ &'5') ! (!*&$(+! 5,( !%!, ! (*5, A!=2, ! ')4&'( CHO ' +* %,(3' ! C*. E $," "*!/, +! ,7+!(/' &,3, *" 5(!&$5$#', (,<, '($, &,((!+5,"$!"#! ' -$, &*5(,+, C*>O.
E (!'$/, ! B!"!$ &,"+#' !: F S*%'#, &45($&,
F C$#('#, ! +,$, F C'(7,"'#, '"=$(, ! +,$,. F A!3;+ +! !35!' N'OH 5'(' '&'$"$)'( ! 3!$,
P(*!7' ! B!"!$ ! 5(,&!$3$!"#, &,3, +! '
FUNDAMENTO DE LA REACCION DE FEHLING
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
E+#' (!'&&$" +! 5(,*&! !" 3!$, '&'$", %*!(#!8 5,( , *! '*",+ &,35*!+#,+ ", (!*,(!+ &,3, ' %(*,+' *! &,"#$!"! *" (*5, &!#,"' 5*!! !",$)'(+! ' ' %,(3' '!=2, '", *'( ' *" %'+, 5,+$#$/,.
R!'&&$" 5,+$#$/' ! ' 5(*!7' ! %!=$" &," *" &'(7,", '",30($&, $7(!
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
C11% L' +'&'(,+'8 *&,+' %(*,+' +," +$,+ 7'"&,+ &($+#'$",+ (!+5,"+'7!+ ! !+'((,, ! &,,( '3'($,3'((" !" ! 5(,&!+'3$!"#, ! ,+ '$3!"#,+. L'+ (!'&&$,"!+ +," '+ +$*$!"#!+: 1 D!(''&$" #0(3$&' ! ')4&'(8 &,"!"+'&$" ' 5H 7'<, %,(3'&$" ! &'('3!, > D!(''&$" '&'$"' ! ' %(*,+' &,"!"+'&$"8 O+&*(!&$3$!"#, &," '3$"'+ 5($3'($'+ %,(3'&$" ! 5$3!"#,+. S1,'&&-"-
E '#, (', ! +,*7$$' !+ !+!"&$' !" ' 5(!5'('&$" ! &,"+!(/'+8 <'!'+8 3!(3!''+8 7!7$'+ <'('7!+. L'+ 3!)&'+ ! ')4&'(!+ 5(,5,(&$,"'" *"' '#' &,"&!"#('&$" ! +$,+ $+*!#,+. L' "'#*('!)' =$(,+&5$&' ! ,+ ')4&'(!+ +! &,((!'&$,"' &," +* +,*7$$' ' %(*,+' &($+#'$"' +! 3!)&' &," ' +'&'(,+' 5'(' 3!<,('( ' +,*7$$' ! 0+#'.
V$1#&-"L'+ +,*&$,"!+ ! +'&'(,+' +," $"#!(3!$'+ !"#(! ' /$+&,+$' ! ,+ <'('7!+ ! '#' %(*,+' ,+ ! *&,+' '#, &,"#!"$, ! '3$,"!+ ", =$(,$)',+.
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
'
'
'$/$'
3$&(,7$'"'
C14#2"42 -&$2%&$"
L' &,"+#'"#! $!0($&' !+ ' 5(,5$!' *! '%!' ' ,+ '$3!"#,+ ' &'!"#'(+! , 5(!5'('(+! 3!$'"#! 3$&(,,"'+. L' &,"+#'"#! $!0($&' ! ' +'&'(,+' ,+ 3,",+'&;($,+ !+ 3*&=, 3;+ '#' *! ' ! ,+ &'(7,=$('#,+ &,35!<,+8 &,3, ' &!*,+'
!
'3$"8
,+
25$,+8
'+
5(,#!2"'+
,#(,+
'$#$/,+.
D!7$, ' *! ' +'&'(,+' #$!"! ' 5(,5$!' ! %,(3'( $5,,+ &*'", +! ='&! *" !"'&!
! =$(!", ' &,"#', &," ! '*'8 +! &,"/$!(#! !" *" $"(!$!"#! $"$+5!"+'7! !" ' %,(3*'&$" ! '$3!"#,+ 3$&(,=,("!'7!+8 5*!+ $"&(!3!"#' ! "$/! ! &'!"#'3$!"#, !" '
+*5!(%$&$!
!
'$3!"#,
'
&,"+$+#!"&$'
&(*<$!"#!
,
!
&'('3!,.
P%10&-"-# "42&1;&-"42#
L' +'&'(,+' 5(!/$!"! ! !#!($,(, ! +'7,( !" '+ %(*#'+ !"'#''+ !/$#' *! '+ '!#'+ +! '(('"&$!". L' +'&'(,+' !" +,*&$" !/$#' ' %,(3'&$" ! -$,+ !" =$!((, !7$, ' +* 7'<' '$/$'.
CONCLUSION :
E" &,"&*+$" !" !+#' 5('$&' +! 5*, $!"#$%$&'( 5,( 3!$, ! (!'&&$,"!+ ! !-#(',+ "'#*('!+ &," ,+ (!'$/,+ ! %!=$" A 8 B B!"!$ &*'", +! ' *" ')4&'( (!*,( &*'", *" ')4&'( ", (!*,(. RECOMENDACION:
S! (!&,3$!"' +!*$( (!'$)'", !+#! #$5, ! '7,('#,($,+ !3,+#('#$/,+ 5'(' *! ! '*3", #!"' *" 3!<,( &,",&$3$!"#, !"#!"$3$!"#, ! ' 3'#!($'. BIBLIOGRAFÍA:
1. $$5!$'. D$+5,"$7! !": =##5:??!+.$$5!$'.,(?$$?R!'&&CB"!M,$+&= >. $$5!$'. D$+5,"$7! !": =##5:??'3!).5"#$&.3!&.!+?37'3?@YQ?5'$"'+?LABORATORIO+?AZUCARES1.=# 3