LABORATORIUM FARMAKOGNOSI-FITOKIMIA FAKULTAS FARMASI UNIVERSITAS HASANUDDIN
LAPORAN “Kromatografi Lapi Tipi! “B"a# Ma$i-ma$i (Leea aquleata)! aquleata) !
OLEH% KELOMPOK VIII NURUL MUTMAINNAH
(N111 10 905)
ISMAWATI
(N111 10 252)
ERWINDA DESRIANI AR
(N111 10 131)
IKA MERDEKAWATI
(N111 10 300)
FAKULTAS FARMASI UNIVERSITAS HASANUDDIN MAKASSAR &'(&
BAB I PENDAHULUAN
I)( Latar B*$a+a,g Berbagai me!"e #r!ma!gra$i memberi#a% &ara 'emiaa% 'a*i%g #+a "i *ab!ra!ri+m #imia, -agaa% "aar%.a e"era%a +%+# "i'aami/ &ara%.a beragam/ m+*ai "ari &ara e"era%a am'ai .a%g aga# r+mi "ari egi #era "a% 'era*aa%/ "a% me!"e i%i "i'a#ai +%+# eia' e%i e%.aa, Me!"e i%i 'ema%$aaa%%.a e&ara *+a +%+# 'emiaa% a%a*ii# "a% 're'arai$, Kr!ma!gra$i *a'i i'i a"a*a +a+ e#%i# 'emiaa% &ara *ama/ "ig+%a#a% e&ara *+a/ er+ama "a*am a%a*ii &am'+ra% .a%g r+mi "ari +mber a*am, Kr!ma!gra$i *a'i i'i *ebi +%gg+* bi*a e+m*a #!%"ii 'emiaa% .a%g berbe"abe"a "i'er*+#a% +%+# me%a%ga%i 'e%ea'a% #a"ar e*+r+ &+'*i#a%/ #are%a e+m*a bea%a 'e%gemba%g .a%g berii berbagai iem 'e*ar+ "a'a *ebi ema "i'a#ai, Ke+%+%ga% *ai%/ ia"a%.a ga%gg+a% 'e*ar+ 'a"a 'e%.e*i"i#a% e&ara $!!meri #are%a 'e*ar+ ebagai $ae gera# e*a "i+a'#a%, emiaa% e&ara #r!ma!gra$i "i*a#+#a% "e%ga% &ara me%g!a#ai# *a%g+%g bebera'a i$a $ii#a +m+m "ari m!*e#+*/ 'a"a iem #r!ma!gra$i/ &am'+ra% .a%g a#a% "i'ia#a% "iem'a#a% "a*am #ea"aa% e"emi#ia%
r+'a ei%gga #!m'!%e%#!m'!%e%%.a ar+ me%+%+##a% "+a "ari #eiga i$a ereb+ .ai+ #e*ar+a%/ a"!rbi/ "a% #eairia%, I)& Ma+" a, T"."a, I)&)( Ma+" P*r/o0aa, U%+# me%gea+i "a% memaami &ara&ara 'emiaa% #!m'!%e% #imia "ari +a+ e#ra# b+a a%ama% mali-mali (Leea aquleata) "e%ga% me%gg+%a#a% #r!ma!gra$i *a'i i'i, I)&)& T"."a, P*r/o0aa, Memia#a% "a% me%gi"e%i$i#ai #!m'!%e% #imia "ari +a+ e#ra# b+a a%ama% mali-mali (Leea aquleata) "e%ga% me!"e KLT, I)1 Pri,ip P*r/o0aa, emiaa% #!m'!%e% #imia .a%g er#a%"+%g "a*am +a+ e#ra# b+a a%ama% mali-mali (Leea aquleata) "e%ga% me!"e KLT ber"aar#a% #e&e'aa% 'arii "a% a"!rbi "ari 4a +i #e"a*am e*+e% "e%ga% 'arameer %i*ai R$ me*a*+i i"e%i$i#ai %!"a .a%g erbe%+# "i baa i%ar U "e%ga% 'a%a%g ge*!mba%g 256 "a% 377, Mem'erega be%+# %!"a #emba*i "e%ga% 'e%.em'r!a% H 2S86, ,
BAB II TIN2AUAN PUSTAKA
II)( T*ori Um"m Kr!ma!gra$i *a'i i'i a"a*a me!"e #r!ma!gra$i &air .a%g 'a*i%g e"era%a, Kr!ma!gra$i a"a*a e#%i# 'emiaa% &am'+ra% ber"aar#a% 'erbe"aa% #e&e'aa% 'erambaa% #!m'!%e% "a*am me"i+m ere%+, a"a #r!ma!gra$i/ #!m'!%e%#!m'!%e%%.a a#a% "i'ia#a% a%ara "+a b+a $ae .ai+ $ae "iam "a% $ae gera#, ae "iam a#a% me%aa% #!m'!%e% &am'+ra% e"a%g#a% $ae gera# a#a% me*ar+#a% 4a #!m'!%e% &am'+ra%, K!m'!%e% .a%g m+"a eraa% 'a"a $ae "iam a#a% eri%gga*, Se"a%g#a% #!m'!%e% .a%g m+"a *ar+ "a*am $ae gera# a#a% bergera# *ebi &e'a,(2) a"a Kr!ma!gra$i *a'i i'i "a% #r!ma!gra$i #era er+'a "a*am a* $ae "iam%.a ber+'a *a'ia% i'i "a% $ae gera#%.a me%ga*ir #are%a #era #a'i*er, erbe"aa%%.a "a*am i$a "a% $+%gi $ae "iam, a"a KLT/ $ae &air *a'ia% i'i (eba* 0/12 mm) .a%g er"iri "ari baa% 'a"a .a%g "i*a'i#a% #e'a"a 'erm+#aa% 'e%.a%gga "aar .a%g biaa%.a erb+a "ari #a&a/ a'i "a'a '+*a erb+a "ari 'e*a '!*imer aa+ *!gam, La'ia% me*e#a #e'a"a 'erm+#aa% "e%ga% ba%+a% baa% 'e%gi#a/ biaa%.a :aS8 6 aa+ ami*+m ('ai) (1), a"a KLT/ 4a 'e%.era' mer+'a#a% *a'ia% i'i erb+# a*+ .a%g "i*a'i#a% 'a"a *em'e%g #a&a/ '*ai# aa+ *!gam e&ara meraa/ +m+m%.a
"ig+%a#a% *em'e%g #a&a, Lem'e%g .a%g +m+m%.a "a'a "ia%gga' ebagai #!*!m #r!ma!gra$i erb+#a "a% 'emiaa% .a%g er&a'ai "a'a "i"aar#a% 'a"a a"!rbi/ 'arii aa+ #!mbi%ai #e"+a e$e#/ erga%+%g "ari e%i 4a 'e%.a%gga/ &ara 'emb+aa% "a% e%i 'e*ar+ .a%g "ig+%a#a% (2), KLT "e%ga% *a'i i'i 'e%+#ar i!% "a'a "ig+%a#a% +%+# 'emiaa% e%.aa '!*ar, er#iraa% i"e%i$i#ai "i'er!*e "e%ga% 'e%gamaa% ber&a# "e%ga% arga R$ .a%g i"e%i# "a% +#+ra% am'ir ama/ "e%ga% me%!!*#a% 4a +i "a% ba#+ 'emba%"i%g 'a"a *em'e%g .a%g ama, erba%"i%ga% ;i+a* +#+ra% ber&a# .a%g "a'a "ig+%a#a% +%+# mem'er#ira#a% #a"ar e&ara emi#+a%iai$ (2), Tii# em'a &am'+ra% "i!!*#a% 'a"a ++%g 'e*a aa+ *embara% "ieb+ ii# aa* "e%ga% &ara me%em'a#a% &+'*i#a% i+ "ia%a "ieb+ 'e%!!*a%, -ari "e'a% 'e*ar+ a"a*a bagia% aa $ae gera# aa+ 'e*ar+ #ei#a ia bergera# me*a*+i *a'ia%/ "a% ee*a 'e%gemba%ga% e*eai / mer+'a#a% i%ggi ma#im+m .a%g "i'er!*e 'e*ar+, eri*a#+ e%.aa ere%+ "i "a*am iem #r!ma!gra$i ere%+ "i%.aa#a% "e%ga% arga R$, A%g#a i%i "i'er!*e "e%ga% membagi ara# .a%g "iem'+ !*e ber&a# *i%ar+ "e%ga% ara# .a%g "iem'+ !*e gari "e'a% 'e*ar+, Ke"+a%.a "i+#+r "ari i# aa* "a% arga R$ beragam m+*ai "ari 0 am'ai 1 (1), A"a "+a me!"e #+a%iai a%a*i "a*am KLT (&!&!# +%+# baa% a%i ra"i!a#i$), erama me*iba#a% e+m*a &ara 'e%g+#+ra% *a%g+%g 'a"a *em'e%g
e'eri
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#eer*iaa%/
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"e%i!meri, Ke"+a me*iba#a% 'ergera#a% a%a*i "ari *em'e%g/ "ii#+i "e%ga% aa' #+a%iai, Mai%gmai%g me!"e mem'+%.ai #e+%+%ga% "a% #er+gia% "a% mem'+%.ai #e"+"+#a% ere%"iri "a*am KLT #+a%iai$, Te#%i# i%i er+ama "ie#a%#a% 'a"a "e%i!meri (3), Ni*ai R$ a"a*a #ara#erii# +a+ #!m'!%e% ei%gga "a'a membe"a#a% a+ "e%ga% *ai%%.a/ %i*ai R$ "i'e%gar+i !*e bebera'a $a#!r "i a%ara%.a<(5) a,
e*ar+ .a%g "ig+%a#a%
b, Baa% 'e%gemba% (e%i "a% #eeba*a% *a'ia%), &, S++, ", Kee%+a% r+a%ga% a#a% 'e*ar+, e, Ke*embaba% +"ara, $, K!%e%rai "a% #!m'!ii *ar+a% .a%g "i'eri#a, g, a%a%g ra.e# migrai, , Se%.aa ai%g "a% 'e%&emara% 'e*ar+, i,
Kei"a#!m!ge%a% *em'e%g,
e%g+#+ra% ber*a%g+%g ebagai beri#+<
R$ =
a"a eb+a *em'e%ga% *a'i i'i eri%g#a*i memi*i#i +ba%i .a%g "iamba#a% #e"a*am%.a/ +'a.a me%gai*#a% 'e%"ara% $*!+r #ei#a
"iberi#a% i%ar +*ra;i!*e (U), I+ berari i#a me%.i%ar#a%%.a "e%ga% i%ar U/ a#a% ber'e%"ar, e%"ara% i%i "i++'i 'a"a '!ii "ima%a ber&a# 'a"a #r!ma!gram bera"a/ me#i'+% ber&a#ber&a# i+ i"a# am'a# berar%a i#a "i*ia "e%ga% maa, I+ berari baa me%.i%ar#a% i%ar U 'a"a *em'e%ga%/ a#a% imb+* 'e%"ara% "ari '!ii .a%g berbe"a "e%ga% '!ii ber&a#ber&a#, Ber&a# am'a# ebagai bi"a%g #e&i* .a%g ge*a',(5) >e* i*i#a a"a*a be%+# "ari i*i#!% "i!#i"a (i*i#a), A!m i*i#!% "i+b+%g#a% !*e a!m !#ige% "a*am r+#+r #!;a*e% .a%g bear, Nam+%/ 'a"a 'erm+#aa% e* i*i#a/ a!m i*i#!% ber*e#aa% 'a"a g+g+ 8H, >a"i/ 'a"a 'erm+#aa% e* i*i#a er"a'a i#aa% Si8H e*ai% Si8Si, erm+#aa% e* i*i#a a%ga '!*ar "a% #are%a%.a g+g+ 8H "a'a membe%+# i#aa% i"r!ge% "e%ga% e%.aae%.aa .a%g e+ai "ie#iar%.a/ ebagaima%a a*%.a ga.a ;a% "er Waa* "a% ara#i "i'!*"i'!*, (6) Kei#a 'e*ar+ m+*ai membaai *em'e%ga%/ 'e*ar+ 'erama a#a% me*ar+#a% e%.aae%.aa "a*am ber&a# .a%g e*a "iem'a#a% 'a"a gari "aar, Se%.aae%.aa a#a% &e%"er+%g bergera# 'a"a *em'e%ga% #r!ma!gra$i ebagaima%a a*%.a 'ergera#a% 'e*ar+, Bagaima%a &e'a%.a e%.aae%.aa "ibaa bergera# #e aa 'a"a *em'e%ga%/ erga%+%g 'a"a< 1, Ke*ar+a% e%.aa "a*am 'e*ar+, Terga%+%g 'a"a bear ara#i a%ara m!*e#+*m!*e#+* e%.aa "e%ga% 'e*ar+,
2, Se%.aa me*e#a 'a"a $ae "iam/ mia*%.a e* i*i#a, Terga%+%g 'a"a bagaima%a bear ara#i a%ara e%.aa "e%ga% e* i*i#a, Se%.aa .a%g "a'a membe%+# i#aa% i"r!ge% a#a% me*e#a 'a"a e* i*i#a *ebi #+a "iba%"i%g e%.aa *ai%%.a a%.a "a'a me%gambi* bagia% i%era#i ;a% "er Waa* .a%g *ema, Kia me%gaa#a% baa e%.aa i%i erera' *ebi #+a "ari e%.aa .a%g *ai%%.a, e%era'a% mer+'a#a% 'embe%+#a% +a+ i#aa% "ari a+ +ba%i 'a"a 'erm+#aa%,(6) e%.era'a% 'a"a #r!ma!gra$i *a'i i'i beri$a i"a# 'erma%e%/ er"a'a 'ergera#a% .a%g ea' "ari m!*e#+* a%ara .a%g erera' 'a"a 'erm+#aa% e* i*i#a "a% .a%g #emba*i 'a"a *ar+a% "a*am 'e*ar+, De%ga% e*a e%.aa a%.a "a'a bergera# #e aa 'a"a *em'e%ga% e*ama a#+ er*ar+ "a*am 'e*ar+, Kei#a e%.aa "iera' 'a"a e* i*i#a+%+# eme%ara a#+ 'r!e 'e%era'a% bere%i"ima%a 'e*ar+ bergera# a%'a e%.aa, I+ berari baa ema#i% #+a e%.aa "iera'/ ema#i% #+ra%g ara#
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ber$*+!ree%i "e%ga% ra"iai U 'a"a 256 %m aa+ 377 %m (7),
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a"a 'ri%i'%.a 'e%am'a#a% %!"a 'a"a U 256 a"a*a $*!+ree%i "ari *em'e%g KLT ereb+ e"a%g#a% "a*am U 377 *em'e%g KLT me%a"i ge*a' e"a%g#a% %!"a a#a% ber$*+!ree%i, a"a U 256 *em'e%g a#a% ber$*+!ree%i "a% %!"a a#a% am'a# me%a"i ge*a', Ha* i%i "iebab#a% #are%a a"a%.a "a.a i%era#i a%ara ia%r U "e%ga% i%"i#a!r $*+!re%i .a%g er"a'a 'a"a *em'a%g, *+!ree%i &aa.a .a%g am'a# mer+'a#a% emii &aa.a .a%g "i'a%&ar#a% !*e #!m'!%e% ereb+ #i#a e*e#r!% .a%g ere#iai "ari i%g#a e%ergi "aar #e i%g#a e%ergi .a%g *ebi i%ggi #em+"ia% #emba*i #e #ea"aa% em+*a ambi* me*e'a#a% e%ergi, (5) Se"a%g#a% 'a"a U 377 %!"a a#a% ber$*+!ree%i "a% *em'e%g a#a% am'a# ge*a', e%am'a#a% %!"a 'a"a U 377 "iebab#a% #are%a a"a%.a "a.a i%era#i a%ara i%ar U "e%ga% g+g+ #r!m!$!r .a%g eri#a !*e a+#!#r!m .a%g a"a'a"a %!"a ereb+, (5) a"a aa "iem'r! me%gg+%a#a% H 2S86/ 'a"a 'ri%i'%.a .ai+ +%+# me%g!#i"ai !*+e!*+ !rga%i# .a%g a#a% %am'a# ebagai ber&a# iam am'ai #e&!#*a&!#*aa%/ ei%gga %!"a "a'a er"ee#i, (5) Tr!+b*e!! 'a"a KLT/ .ai+< 1, N!"a .a%g bere#!r, 2, Kea*aa% 'arii 3, e%!!*a% .a%g er*a*+ eba* 6, E#ra# .a%g er*a*+ #e%a*,
BAB III METODE KER2A
III)( A$at a, Ba#a, III)()( A$at B!!* E*+e%/ -e*a :amber/ -e*a ia*/ -e*a U#+r/ Lam'+ U 256@377/ Lem'e%g #r!ma!gra$i (i*i#a ge*)/ e%!!*/ e%.em'r! KLT/ "a% i%e, III)()& Ba#a, Air +*i%g/ Aam S+*$a 10/ E#ra# Sam'e*/ E*+e%/ Ea%!* 95 / Kera ari%g/ "a% *a KLT - 256, III)& 3ara K*r.a III)&)( P*riapa, L*mp*,g KLT a, Samp*$ Diia'#a% a*a "a% baa%, Dia#i$#a% *em'e%g KLT "i !;e% 'a"a ++ 105!110!: e*ama 1 am, Dig+%i%g *em'e%g e+ai +#+ra% .a%g "i#ee%"a#i (biaa%.a +#+ra% 2 C &m), Dia%"ai baa baa "a% baa aa "e%ga% 'e%i* "e%ga% +#+ra% 1 &m "a% 0/5 &m, Dima+##a% $ae gera#@e*+e% #e "a*am &amber am'ai #ira#ira #ei%ggia% #+ra%g "ari 1 &m, Die%+#a% &amber "e%ga% me%gg+%a#a% #era ari%g, Di*ar+#a% e#ra# .a%g e*a "i'arii (e#ra# b+a%!* e%+ air "a% e#a%) "a% e#ra# aa*
#e "a*am 'e*ar+ .a%g e+ai am'ai "i'er!*e #e'e#aa% .a%g e+ai/ "i "a*am b!!* ;ia*, Di!!*#a% e#ra# am'e* 'a"a baa baa *em'e%g "e%ga% me%gg+%a#a% 'i'a #a'i*er, Dima+##a% #e "a*am &amber .a%g e*a "ie%+#a% "e%ga% e*+e%, Dibiar#a% *em'e%g ere*+i am'ai baa aa/ #em"+ia% a%g#a "a% #eri%g#a%, III)&)& I*,tifi+ai KLT Diamai %!"a .a%g m+%&+* "e%ga% me%gg+%a#a% 'e%am'a# %!"a *am'+ U 256@377 %m "a% H 2S86 10, Di&aa ar%a %!"a .a%g m+%&+* "a% i+%g %i*ai R$,
BAB VI HASIL PENGAMATAN
IV)( Ta0*$ P*,gamata, a, E*+e% He#a% < Ei* = Sam'e* E#ra# b+a a%ama% mali-mali (Leea aquleata)
K!"e N!"a Aa* Lar+ B+a%!* Lar+ Hea%
Ni*ai R$ 1,1
War%a N!"a U 256 U 377 :!#*a :!#*a :!#*a :!#*a :!#*a :!#*a
b, E*+e% He#a% < Ei* = Sam'e* E#ra# b+a a%ama% mali-mali (Leea aquleata)
K!"e N!"a Aa* Lar+ B+a%!* Lar+ Hea%
Ni*ai R$ 1,1
War%a N!"a U 256 U 377 :!#*a :!#*a :!#*a :!#*a :!#*a :!#*a
IV)& Gam0ar Profi$ KLT a) E$"*, H*+a, % Eti$ 4 a"a U 377
a"a U 256
IV)1
P*r#it",ga, ,i$ai Rf >ara# .a%g "iem'+ !*e %!"a R$ = >ara# .a%g "iem'+ !*e e*+e%
a, E*+e% He#a% < Ei* = b, E*+e% He#a% < Ei* =
BAB V PEMBAHASAN
a"a 'er&!baa% i%i "i*a#+#a% 'emiaa% #!m'!%e% #imia .a%g "i#a%"+%g !*e e#ra# b+a a%ama% mali-mali (Leea aquleata) "e%ga% me%gg+%a#a% me!"e #r!ma!gra$i *a'i i'i ber"aar#a% #e&e'aa% 'arii "a% a"!rbi "ari 4a +i #e "a*am e*+e% "e%ga% 'arameer R$ "ari %!"a .a%g erbe%+#, Lem'e%g .a%g "ig+%a#a% me%gg+%a#a% a"!rbe% .a%g erb+a "ari i*i#a ge*, era*aa% .a%g "ig+%a#a% 'a"a KLT i%i me*i'+i +a+ *em'e%g i'i, De%ga% ba+a% a*a i%i baa% !rbe% "a'a "ib+a raa 'a"a 'e*a "a% "a'a "i*a'i#a% "e%ga% #eeba*a% .a%g "ii%gi%#a%, e*a i%i mem+%g#i%#a% e+m*a *ar+a% "i'eri#a "a% *ar+a% 'emba%"i%g "i!!*#a% 'a"a ii# aa*, Se*ai% 'e*a +ga "ig+%a#a% bea%a #r!ma!gra$i "ari baa% emb+ &aa.a "e%ga% ++' ra'a, Bea%a "i*a'ii #era ari%g "a% e+m*a #e&i* $ae gera# "i+a%g#a% +%+# 'e%e%+a% #era "a% 'a"a "aar bea%a "iii "e%ga% 'e*ar+ 'e%gemba%g ei%ggi #+ra%g "ari 1 &m, Di++' "a% "ibiar#a% e%+ "e%ga% e*+e%, E*+e% .a%g "i'i*i a"a*a e#a% "a% ei*, Dig+%a#a% "+a 'e*ar+ .a%g berbe"a/ "e%ga% ++a% agar 'emiaa% "a'a era"i e&ara !'ima* #are%a "a.a e*+i &am'+ra% "+a 'e*ar+ i%i "a'a m+"a "ia+r e"emi#ia% r+'a, He#a% beri$a %!%'!*ar/ "a% ei* beri$a '!*ar,
8*e #are%a i+/ e#a% "iara'#a% "a'a me%ari# %!"a .a%g beri$a %!%'!*ar/ "a% ei* "a'a me%ari# %!"a .a%g beri$a '!*ar, A"!rbe% .a%g 'a*i%g ba%.a# "ig+%a#a% "a*am #r!ma!gra$i *a'i i'i a"a*a i*i#a ge* "a% a*+mi%i+m !#i"a, Si*i#a ge* +m+m%.a me%ga%"+%g baa% ambaa% #a*i+m +*$a +%+# mem'eri%ggi "a.a *e#a%.a, Si*i#a ge* "ig+%a#a% ebagai a"!rbe% +%+# #r!ma!gra$i e%.aae%.aa %era*/ aam "a% baa, Se*ai% i+ i*i#a ge* mem'+%.ai e$e# 'emiaa% me*a*+i 'r!e a"!rbi "a% 'arii, Lar+a% 4a +i "i!!*#a% 1 &m "ari baa "a% mi%im+m 0/5 &m "ari ii 'e*a/ e"emi#ia% r+'a ei%gga era"i %!"a era+r .a%g ma#im+m ber"iameer 7 mm/ ea'i 'a"a 'er&!baa% i%i .ara ereb+ i"a# "i'erai#a% ei%gga *em'e%g .a%g "ig+%a#a% *ebar%.a a%ga #e&i*, e%!!* .a%g "ig+%a#a% ebai#%.a ber"iameer 0/1 mm ? 1 mm/ ei%gga *ar+a% 4a +i .a%g "ig+%a#a% +ga e+ai "e%ga% a'a .a%g "ii%gi%#a%, See*a "i!!*#a%/ 'e*a "i+a'#a% agar , La*+ 'e*a "i*ea##a% ;eri#a* "a*am bea%a #r!ma!gra$i "a% ii# aa* ar+ ea' bera"a "iebe*a aa 'erm+#aa% $ae gera#, Bea%a "i++' "a% "i"iam#a% i%gga $ae gera# me%&a'ai baa aa, >i#a $ae gera# +"a me*eai ra.e# .a%g "iberi#a% "a*am m!%!gra$i/ 'e*a "i#e*+ar#a% "ari bea%a "a% "i#eri%g#a% "i +"ara, Taa' era#ir .a%g "i*a#+#a% a"a*a 'e%ee#ia% ber&a#, Ha* i%i "i*a#+#a% "e%ga% "+a &ara/ .ai+ 'e%gamaa% "ibaa *am'+ U "e%ga% 'a%a%g ge*!mba%g 256 %m "a% 377 %m "a% 'e%.em'r!a% "e%ga% H 2S86,
T++a% 'e%.em'r!a% .ai+ +%+# me%g!#i"ai !*+e!*+ !rga%i# .a%g a#a% %am'a# ebagai ber&a# iam am'ai #e&!#*a&!#*aa%, a"a 'e%"ee#ia% "ibaa i%ar U 256/ *em'e%g a#a% ber'e%"ar "a% %!"a a#a% ge*a', Ha* i%i #are%a era"i%.a i%era#i a%ara i%ar U "a% i%"i&a!r .a%g a"a 'a"a *em'e%g/ e"a%g#a% 'a"a U 377/ *em'e%g a#a% ge*a' "a% %!" a#a% ber'e%"ar, Dii%i era"i i%era#i a%ara i%ar U "e%ga% g+g+ #r!m!$!r .a%g a"a 'a"a am'e*, -+g+ #r!m!$!r mer+'a#a% g+g+@a!m .a%g "a'a me%a%g#a' i%ar U aa+ am'a#, Harga R$ mer+'a#a% 'arameer #araerii# #r!ma!gra$i #era "a% #r!ma!gra$i *a'i i'i, Harga i%i mer+'a#a% +#+ra% #e&e'aa% migrai +a+ e%.aa 'a"a #r!ma!gram "a% 'a"a #!%"ii #!%a% mer+'a#a% beara% #araerii#"a% re'r!"+#ibe*, Harga R$ "i"e$i%ii#a% ebagai 'erba%"i%ga% a%ara ara# e%.aa "ari ii# aa* "a% ara# e'i m+#a 'e*ar+ "ari ii# aa*, Ter"a'a berbagai $a#!r .a%g "a'a mem'e%gar+i %i*ai R$/ ma#a #ea*aa% "a*am me*a#+#a% 'era#i#+m i%i ea' mei a"a, Mia*%.a ++ +"ara 'a"a aa 'ra#i#+m "a% #e*embaba% +"ara,
BAB VI PENUTUP
VI)( K*imp"$a, Keim'+*a% .a%g "a'a "iari# "ari 'er&!baa% i%i a"a*a %i*ai R$ .a%g "i'er!*e "ari ai* 'e%gamaa% a"a*a 'a"a e#ra# e#a% ebear 1,1
VI)& Sara, Diara'#a% a*aa*a "i *ab!ra!ri+m "a'a "i*e%g#a'i ei%gga 'ra#i#+m "a'a bera*a% "e%ga% e$e#i$ "a% e$eie%,
DAFTAR PUSTAKA
1, -rier/ >,R,/ "##, 1991,
Kromatografi , Ba%"+%g< e%erbi I%i+
Te#%!*!gi, Ha*, 1/ 7/ C, 2, Die% 8M, 1995, Farmakope Indonesia, Edisi IV , >a#ara< De'areme% Keeaa% RI, Ha*, 65/ 67/ 50/ 1002 3, M+%!%/ >,R,,1991, Analisis Farmasi, Bagian B, S+raba.a< Air*a%gga U%i;eri. re, Ha*, 125/ 12C, 6, '<@@,&rib",&!m@m!bi*e@"!&@5C576555F G+er.=#r!ma!gra$i*a'ii'i , Dia#e a%gga* 20 A'ri* 2012 5, '<@@,&rib",&!m@m!bi*e@"!&@366C69F G+er.=#r!ma!gra$i*a'ii'i, Dia#e a%gga* 20 A'ri* 2012 7, M+*a/ M,/ S+arma%, 1995, Analisis Instrumental ) S+raba.a< Air*a%gga
U%i;eri. re
