LAPORAN INOKULASI BAKTERI

LAPORAN INOKULASI BAKTERI LABORATORIUM MIKROBIOLOGI FARMASI JURUSAN FARMASI POLITEKNIK KESEHATAN KEMENKKES MAKASSAR

 LAPORAN INOKULASI BAKTERI

OLEH : KELOMPOK 4 KELAS REGULER A. 

SITI ANIAH HARDIATY

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SRY REZKY AMALIAH SUPARMIN ROMI TUTI ERNAWATI VERA FEBRIANTI  YANTI  YA NTI SARI SYAM SYAM

JURUSAN FARMASI POLITEKNIKKE SEHATAN KEMENKKES MAKASSAR 2011/2012

BAB I PENDAHULUAN

I.1. LATAR BELAKANG Dala Da lam m

tekn te knik ik

biak bi akan an

m!n m !nii

ti"a ti "ak k

#a$a #a $a

"i% "i %e! e!l lka kan n

ba&a ba &aim iman ana a

mem%e!'le( #at biakan )an& m!ni* teta%i $&a ba&aimana memeli(a!a #e!ta #e !ta me men+e n+e&a &a( ( %e %en+e n+ema! ma!an an "a "a!i !i la la! !. In' In'kl kla# a#ii "i "imak mak#" #"kan kan nt ntk  k  menmb(kan* menmb(k an* me!ema$akan mik!'ba "an men"a%atk men"a%atkan an %'%la#i mik!'ba )an& m!ni. In'kla#i a"ala( %eke!$aan memin"a(kan bakte!i "a!i me"im )an& lama ke me"im )an& ba! "en&an tin&kat ketelitian )an& #an&at tin& ti n&&i &i..

Me"i Me "ia a

nt n tk k

memb me mbia iakk kkan an

bakt ba kte! e!ii

(a! (a !#l #la( a(

#te te!i !ill

#ebe #e bel lm m

"i&nakan. Pen+ema!an te!tama be!a#al "a!i "a!a )an& men&an"n& ban)ak ban )ak mi mik!' k!''!& '!&ani ani#m #me. e. Pe Pemin min"a( "a(an an bia biaka kan n mik mik!'b !'ba a )an )an& & "i "ibi biakk akkan an (a!# #an&at (ati,(ati "an mema memat(i t(i %!'# %!'#e"! e"! lab'!at'!im lab'!at'!im a&a! ti"a ti"ak  k  te!$a" te! $a"ii k' k'nta ntamin mina# a#i. i. Ol Ole( e( ka ka!en !ena a it it** "i "i%e %e!l !lkan kan te tekn knik, ik,tek teknik nik "a "alam lam %embiakan mik!''!&ani#me )an& "i#ebt "en&an teknik in'kla#i biakan. Isols! "#$%&! '%&()#* s($( +& (*$(# '%'!s,#* $( '%'!*-,#* '!#&o" $%&$%*$( -&! l!*.#(*.* s%,!*.. -!)%&ol%, #(l$(& $( "!#* '(&*! - "%"%&) +& ('(' *. -)$ -!l#(#* -%*.* +& .o&%s*s$%# )l$%3 +& $"(& $" (&* * $ $( ( $( $(*. *.)o( )o(&& )l )l$% $%33 s%& s%&$ $ '! '!#&o #&o'* '*!)( !)(l$ l$o& o&$,% $,% '!+ '!+&o' &o'*! *!)(l )(l$o $o&  & 

'%$,o-s l!'32001 S%+& l'!3 "#$%&! -! l' -!$%'(#* -l' )o)(ls! +')(&* H* -l' #%-* $%&$%*$( s4 )o)(ls! !*! -!$%'(#* -l' #%-* $%&$%*$( s4 )o)(ls!!*! -!$%'(#* -l' #%-* '(&*!  U*$(# -)$ '%')%l4&! s!5$ "!#*3 'o&5olo.!3 -* s!5$ 5l!*3 '# o&.*!s'% *. #* -!$%l!$! ,&(s -)$ -!)!s,#* I*! "%&&$! ",6 ,&(s - "!#* '(&*! *. ,* '%*.*-(*. s$( 4%*!s "#$%&! s4 U*$(# '%')%&ol%, "!#* '(&*! -)$ -!l#(#* )%*.%*+%&* -%*.* '%*..(*#* ",* +!& $( )-$ P- '(l* -!.(*#* .%l$!* s%".! ",* )%'-$ T%#*!# (*$(# '%')%&ol%, "!#* '(&*! - 7 +&3 !$(8 $%#*!# )%*..o&%s* .&3 $%#*!# .& $(*.3 $%#*!# .& s%"& T%#*!# !*o#(ls! '%&()#* s($( )%#%&4* '%'!*-,#* "#$%&! -&! '%-!(' *. l' #% '%-!(' *. "&( -%*.* $!*.#$ #%$%l!$!* *. s*.$ $!*..! D%*.* -%'!#!* #* -!)%&ol%, "!#* '!#&oo&.*!s'% *. -)$ -!.(*#* (*$(# )%'"%l4&* '!#&o"!olo.! P- )&#$!#(' !*! #* -!l#(#* $%#*!# !*o#(ls! "!#* '!#&oo&.*!s'% )- '%-!(' s$%&!l (*$(# '%')%l4&! '!#&o"!olo.! -%*.* s$( #(l$(& '(&*! s4 I-%*$!5!#s! "!#* '!#&oo&.*!s'% s%&!*.#l! '%'%&l(#* )%'!*-,* #% "!#* s%.& $*) $%&4-! )%*+%'&* P%'!*-,* '!#&oo&.*!s'% !*! -!l#(#* -%*.* $%#*!# s%)$!# (*$(# '%')%&$,*#* #%'(&*!* "!#* s%l' )%'!*-,* "%&(l*. #l! M!#&oo&.*!s'% -)$ -!$('"(,#* -l' "!#* +!& $( )-$ K%#%&(,* -l' #l-( '%*(*4(##* $%&4-!* )%&$('"(,* '!#&oo&.*!s'% B!l '!#&oo&.*!s'% '%*(')(# )- -s& $"(*. '# #* '%'"%*$(# s%-!'%*3 s%-*.#* )- )%&'(#* #l-( )%&$('"(,** $%&l!,$ s%".! )%l!#%l S%l!* -l' '%-! +!&3 '!#&oo&.*!s'% 4(. '%')%&l!,$#* )%&$('"(,* -%*.* +!&! $%&$%*$( -l' "!#* )-$ s%)%&$! .& '!&!*. $( l%')%*.* .& A.& '!&!*. l9!'* -!.(*#* (*$(# '%*!')* "!#* '(&*! s%-*.#* .&  l%')%*.* l9!'* -!.(*#* (*$(# '%'(&*!#* '!#&oo&.*!s'%

I2 RUMUSAN MASALAH B.!'* +& '%*.!sols! -* '%l!,$ '!#&oo&.*!s'% -!s%#!$& #!$ -* ".!'*

+&

'%*.!*o#(ls!

'!#&oo&.*!s'%

*.

'(&*!

-*

'o&5olo.!* : I7 TUJUAN PRAKTIKUM U*$(# '%*.%$,(! -* '%','! +& '%*.!*o#(ls! '!#&oo&.*!s'% U*$(# '%*.'$! ,s!l )%&%'4* '!#&oo&.*!s'%

'%l!,$

BAB II TIN-AUAN PUSTAKA P%**'* "#$%&! $( "!s -!s%"($ 4(. !*o#(ls! -l, )%#%&4* '%'!*-,#* "#$%&! -&! '%-!(' *. l' #% '%-!(' *. "&( -%*.* $!*.#$ #%$%l!$!* *. s*.$$!*..! U*$(# '%l#(#* )%**'* "#$%&! !*o#(ls! $%&l%"!, -,(l( -!(s#* .&s%'( l$ *. - -l' ,("(*.** -%*.* '%-!(' .&  $%$) s$%&!l3 ,l !*! .&'%*.,!*-&! $%&4-!* #o*$'!*s! D6!4os%)($&o3 1;;< A- "%"%&) $,) *. ,&(s -!l#(#* s%"%l(' '%l#(#* $%#*!# )%**'* "#$%&! !*o#(ls! !$( 8 1. Menyiapkan ruangan R(*. $%')$ )%**'* "#$%&! ,&(s "%&s!, -* #%-** ,&(s s$%&!l .&  $!-# $%&4-! #%sl,* -l' )%*.'$* $( )%&+o"* -l' l"o$&!(' )%'"($*s%&(' =#s!* -* s%".!* I*o#(ls! -)$ -!l#(#* -l' s%"(, #o$# #+ %*+s$  (-& *. l%6$ -l' #o$# $%&s%"($ -!l%6$#* s&!*.* '%ll(! s($( 4l*.& $%#%* s!*& (l$&=!ol%$ P%l+9&3 1;<> 2. Pemindahan dengan dengan pipet  ?& !*! -!l#(#* -l' )%*%l!-!#* !& '!*(' $( )- )%*%l!-!#* (*$(# -!'"!l1 'l +o*$o, *. #* -!%*+%&#* ol%, !& s%"*# ;; 'l '(&*! P%l+9&3 1;<> 3. Pemindahan dengan kawat inokulasi   #6$ !*o#(ls! s%"l!#* -&! )l$!* $( *!#%l (4(*.* "ol%, l(&(s 4(."ol%, "%&()

#olo*.*

*.

-!'%$&*

1@7''

Dl'

'%l#(#(*

)%**'*

"#$%&!#6$ !*! $%&l%"!, -,(l( -!)!4&#* s%-*.#* s!s* $(*.#! +(#() -!l%6$#* *l)! s4 s%$%l, -!*.!* #%'"l! #6$ !$( -!s%*$(,#* l.! -l' *l P%l+9&3 1;<> Teknik In'kla#i A"a bebe!a%a met'"e )an& "i&nakan ntk men&i#'la#i biakan m!ni mik!''!&ani#me )ait : 

Met'"e &'!e#

Teknik ini lebi( men&ntn&kan $ika "itin$a "a!i #"t ek'n'mi "an akt*

teta%i

meme!lkan

ket!am%ilan,ket!am%ilan

)an&

"i%e!'le(

"en&an lati(an. Pen&&'!e#an )an& #em%!na akan men&(a#ilkan k'l'ni )an& te!%i#a(. In'klm "i&'!e#kan "i %e!mkaan me"ia a&a! nt!ien "alam +aaan %et!i "en&an $a!m %in"a( /l% in'kla#i0. Di anta!a &a!i#, &a!i# &'!e#an akan te!"a%at #el,#el )an& +k% te!%i#a( #e(in&&a "a%at tmb( men$a"i k'l'ni. a!a %en&&a!i#an "ilakkan %a"a me"im %embiakan %a"at bentk  lem%en&. Bila "ilakkan "en&an baik teknik inila( )an& %alin& %!akti#. Dalam

%en&e!$aann)a

lab'!at'!im

ta%i

te!ka"an&

t$ann)a

#ama

be!be"a )aiit

%a"a

ntk

ma#in&,ma#in&

membat

&'!e#an

#eban)ak mn&kin %a"a lem%en& me"im %embiakan. A"a bebe!a%a teknik "alam met'"e &'!e#an* )akni : 

G'!e#an T

b.

Go&%s* #(-&*

+. G'!e#an !a"ian

- Go&%s* s!*'"(*.



Met'"e teba! Setete# in'k'lm "iletakan "alam #eba( me"im a&a! nt!ien "alam +aan %et!i"i#( "an "en&an men&&nakan batan& ka+a )an& ben&k'k "an #te!il. In'kla#i it "i#eba!kan "alam me"im batan& )an& #ama "a%at "i&nakan "a%at men&in'kla#ikan %in&&an ke"a ntk "a%at men$amin %en)eba!an bakte!i )an& me!ata "en&an baik. Pa"a bebe!a%a %in&&an akan mn+l k'l'ni k'l'ni )an& te!%i#a(,%i#a(.



Met'"e tan& I#'la#i

men&&nakan

melakkan

me"ia

%en&en+e!an

+ai!

a"ala(

"en&an

+a!a

%en!nan

%en&en+e!an.

$mla(

Da#a!

mik!''!&ani#me

#e(in&&a %a"a #at #aat (an)a "itemkan #at #el "i "alam tabn& /2ina!ni* 1330. 

Met'"e t#k  Met'"e t#k )ait "en&an "en&an +a!a menete#kan ata men#kan $n&

$a!m

'#e

)an&

"i"alamn)a

te!"a%at

in'k'lm*

kem"ian

"ima#kkan ke "alam me"ia /2ina!ni* 1330. Pe!be"aan In'kla#i -am! "an Bakte!i 1. In'kla#i $am! men&&nakan $a!m '#e bentk batan&. Hi5a )an& be!bentk #e%e!ti benan& m"a( "iambil "en&an $a!m '#e batan& "an m"a( #ekali tmb( "i "alam #at me"ia. 6. In'kla#i bakte!i men&&nakan $a!m '#e bentk blat. Pa"a $n& $a!m '#e )an& be!bentk blat* bakte!i akan "a%at te!ambil "alam $mla( )an& !elati5 ban)ak /R'(imat* 67760. T%#*!# Isols! D* P%'(&*!* T%#*!# !sols! '!#&o" (*$(# '%'!s,#* -* '%*.!-%*$!5!#s!3 4%*!s '!#&o" -&! "!#* -)$ -!#ls!5!#s!#* "%&-s&#* s!5$ )%&$('"(,* *. *')# )- '%-! M%$o-% )%**'* )- .& 

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