Biofuge 100/120V
SERVICE MANUAL Cat. No. 12005522
TABLE OF CONTENTS Section 1 2 2.1 2.2 2.3 2.4 2.5 2.6 2.7
Title OPERATING INSTRUCTIONS (not part of this manual) BROSHURE SERVICE Servicing Schedule Trouble Shooting Test Points Mounting and adjustment of the torsion spring Assembly and adjustment of the drive Cleaning of Instrument Parts Electrical Safety Check
3 3.1 3.2 3.3
FUNCTIONAL DESCRIPTION Block Functions Main Board Functions Key and Indication Board
4
4.7
DIAGRAMS For units with 120V mains voltage Block Diagram Wiring Diagrams Wiring Connection Diagrams For units with 100V mains voltage Block Diagram Wiring Diagrams Wiring Connection Diagrams For units with 100V or 120V mains voltage Main Board, Component Plan, Wiring Diagram
5 5.1 5.2 5.3 5.4 5.5 5.6
DISASSEMBLY OF INSTRUMENT PARTS Housing / Casing Parts Sealing Elements Mechanical Components Electrical Components Drive Components Components of Cooling Plant
6 6.1 6.2 6.3 6.4
SPARE PART FIGURES AND LISTS Break down drawings Mounting and adjustment of the torsion spring Mounting of the temperature sensor Spare Part Lists
7
CALIBRATION PROCEDURE
8
TECHNICAL BULLETINS
4.1 4.2 4.3 4.4 4.5 4.6
Edition: 01 17.11.02 MH
Page
2-1/2 2-3/5 2-6 2-7 2-8 2-8 2-9
3-1 3-1/3 3-4
4-1 4-2/3 4-4/6 4-7 4-8/9 4-10/12 4-13/19
5-1 5-2 5-3 5-4/5 5-6 5-7/8
6-1/3 6-4 6-5/6
MICROLITRE CENTRIFUGES HERAEUS® BIOFUGE® PICO AND BIOFUGE® FRESCO Compact, fast and quiet
Typical Applications ● Pelleting DNA and RNA ● Pelleting of PCR amplified nucleic acids ● Antibody and protein precipitates ● Enzymatic tests ● Removing cell debris from cell homogenates ● Bacterial and yeast cell fractionation
The microlitre centrifuges in the Biofuge® family are specially made to handle biological separation tasks. Whether non-refrigerated (Biofuge® pico) or refrigerated (Biofuge® fresco), these centrifuges are indispensable in the modern biological laboratory. Heraeus® centrifuges meet all current European Community guidelines and are CE labelled.
Biofuge® pico ● Incredibly small microlitre centrifuge ● Short braking and acceleration times ● Ergonomic design ● Max. capacity: 24 x 1.5/2 ml or 4 PCR-strips or 32 individual PCR-tubes ● Max. speed: 13,000 rpm ● Max. RCF: 16,060 x g ● Aerosol tight “Easy-Twist-On” lid
Biofuge® fresco ● The smallest refrigerated centrifuge for microlitre tubes ● Ultra-quiet standstill cooling ● Powerful CFC-free refrigeration unit ● Max. capacity: 24 x 1.5/2 ml or 4 PCR-strips or 32 individual PCR-tubes ● Max. speed: 13,000 rpm ● Max. RCF: 16,060 x g ● Aerosol tight “Easy-Twist-On” lid
BIOFUGE® PICO AND FRESCO Compact design Create space in your laboratory. Take advantage of the 23 x 33 cm footprint of the small Biofuge® pico, which can be integrated into any workplace. The minimum height of the pico casing makes it easier to load the rotor.
High performance The brushless induction motor runs quietly. In 15 seconds or less, the rotor accelerates to 16,000 x g, which is sufficient for all standard applications. A “quick run” key is used for short processing runs.
The refrigerated Biofuge® fresco, with a footprint of 29 x 45 cm, is scarcely larger than many non-refrigerated centrifuges.
The high-powered refrigeration unit of the Biofuge® fresco with stand by cooling ensures temperatures beneath 0 °C even at maximum speed.
Ergonomic design The smooth design of the casing and the modern keypad control are easy-toclean. The straightforward arrangement of the keys, with icon labels and the EASYcontrol™ microprocessor controller, make it simple to operate. One-touch lid opening allows for easy access to the rotor.
ROTORS AND ACCESSORIES FOR BIOFUGE® PICO AND FRESCO Both centrifuges are equipped with a 24-place microlitre rotor suitable for 1.5 or 2 ml tubes and optimised for applications in the biological sciences. The polypropylene plastic rotor can be autoclaved and offers extensive chemical resistance. The Easy-Twist-On aerosol tight lid (included) offers exceptional comfort and safety.
MIKROLITRE ROTOR INCL. EASY-TWIST-ON AEROSOL TIGHT LID Biofuge® pico
Optional features include adapter sets enabling centrifugation of all commercially available microlitre tubes, from 0.6 ml to 0.2 ml PCR-tubes.
Biofuge® fresco
Max. capacity
ml
24 x 1.5/2.0
24 x 1.5/2.0
Max. speed
rpm
13,000
13,000
Max. RCF
xg
16,060
16,060
Acceleration time
s
15
15
Braking time
s
12
16
Max. radius
cm
8.5
8.5
Reducer sleeves
Order numbers
24 reducer sleeves for 0.6/0.5 ml microtubes
76003758
24 reducer sleeves for 0.4/0.25 ml microtubes
76003759
24 reducer sleeves for 0.2 ml microtubes
76003750
PCR-ROTOR INCL. EASY-TWIST-ON AEROSOL TIGHT LID
ORDER NO. 75003327
Biofuge® pico Max. capacity
With this additional rotor you can easily spin smaller sample volumes especially 4 PCR-strips (8 tubes each) or 32 individual PCR-tubes.
ORDER NO. 75003328
Biofuge® fresco
4 PCR-strips or
4 x 8 PCR-strips or
32 individual 0.2 ml PCR-tubes
32 individual 0.2 ml PCR-tubes
Max. speed
rpm
13,000
13,000
Max. RFC
xg
12,846
12,846
Acceleration time
s
15
12
Braking time
s
15
16
Max. radius
cm
6.8
6.8
TECHNICAL DATA Biofuge® pico
Biofuge® fresco
Designation
Microlitre centrifuge
Refrigerated microlitre centrifuge
Max. RCF
16,060 x g
16,060 x g
Max. speed
13,000 rpm adjustable in 100 rpm increments
13,000 rpm adjustable in 100 rpm increments
Min. speed
2,000 rpm
2,000 rpm
Control
Microprocessor-controlled via EASYcontrol™
Microprocessor-controlled via EASYcontrol™
Drive
Frequency-controlled induction drive
Frequency-controlled induction drive
Acceleration/delay
–
–
Noise level at max. speed
< 55 db (A)
< 55 db (A) standstill cooling < 45 db (A)
Run time
1 – 99 min, continuous operation, quick run
Program memory
The most recently entered data remains in the memory.
1 – 99 min, continuous operation, quick run The most recently entered data remains in the memory.
Construction
Galvanised steel chassis with
Plastic casing with galvanised steel chassis
surface-mounted plastic casing
and sheet steel lid, stainless steel rotor bowl
Safety features
Lid locks and interlocks
Lid locks and interlocks
Temperature range
–
–9 bis +40 °C
Min. temp. at max. speed
–
0 °C (ambient temp. = 25 °)
Dimensions (HxWxD)
206 x 233 x 335 mm
305 x 290 x 450 mm
Weight (without rotor) Power consumption Standards
8.1 x 9.2 x 13.2 inch
12.0 x 11.4 x 17.7 inch
9.7 kg
27 kg
21.3 lbs
59.4 lbs
150 W
270 W
Manufactured and tested in conformity with EN 61 010-1, EN 61 010-2-020, EN 50 081-1, EN 50 082-1 Not subject to testing in accordance with UVV VBG 7z
Refrigerant
–
ORDER NUMBERS ®
Biofuge pico
incl. 24-place microlitre rotor 75003328
R 134 a (CFC-free)
230 V/50–60 Hz
110–120 V/60 Hz
75003235
75003236
75005521
75005522
with Easy-Twist-On aerosol tight lid Biofuge® fresco
incl. 24-place microlitre rotor 75003328 with Easy-Twist-On aerosol tight lid
Your Sales Contact
For Ordering or Technical Information Asia Pacific Kendro Laboratory Products (H.K.) Limited · Hong Kong · Tel. +852 2711 3910 · Fax +852 2711 3858 ·
[email protected] Europe, Middle East, Africa Kendro Laboratory Products International Sales · Hanau · Germany · Tel. +49 (0) 1805 - 536 376 · Fax +49 (0) 1805 -112 114 ·
[email protected] Latin America Kendro Laboratory Products International Sales · Newtown, CT · USA · Tel. +1 203 - 270 2080 · Fax +1 203 - 270 2210 ·
[email protected] North America Kendro Laboratory Products · Newtown, CT · USA · Tel. +1 800 - 522 7746 · Fax +1 203 - 270 2166 ·
[email protected] http://www.kendro.com
Quality Products – Lifetime Care Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards. ©2002 Kendro Laboratory Products. All Rights Reserved. Printed in Germany 2C 09/02 4t Druckerei
Z pico 5e
Internet
Heraeus Pico Fresco Brochure
18/11/02
11:59 am
Page 3
Benchmarks for Size and Value. Pico and Fresco Microcentrifuges SORVALL
®
Heraeus Pico Fresco Brochure
18/11/02
11:59 am
Page 4
Small footprints, BIG value
Kendro Laboratory Products’ SORVALL® Pico and Fresco are the smallest, quietest and quickest microcentrifuges going around. Their sleek, lightweight designs maximize your available bench space, and deliver all the performance you will ever need, year in and year out, for a host of separation tasks in the microliter sample range.
SORVALL Pico
SORVALL Fresco
• Compact 9" x 13" (233 mm x 335 mm) footprint • 13,000 rpm (16,060 x g) • Short braking and acceleration times • High capacity 24 x 1.5/2.0 ml biocontainment rotor • NEW PCR strip rotor • NEW Easy-Twist-On biocontainment lid • Microprocessor-based keypad control • Whisper-quiet, maintenance free brushless drive
• The smallest refrigerated centrifuge available • 13,000 rpm (16,060 x g) • 11" x 18" (290 mm x 450 mm) footprint • High capacity 24 x 1.5/2.0 ml biocontainment rotor • NEW PCR strip rotor • NEW Easy-Twist-On biocontainment lid • Ultra-quiet standstill cooling • Precise temperature control from -9°C to 40°C • Whisper-quiet, maintenance free brushless drive
Typical applications • Pelleting DNA and RNA • Pelleting of PCR-amplified nucleic acids • Antibody and protein precipitates • Enzymatic tests • Removing cell debris from cell homogenates • Bacterial and yeast cell fractionation
Heraeus Pico Fresco Brochure
18/11/02
11:59 am
Page 5
Compact microcentrifuges for Life Science
Choice of rotors Microliter and PCR strip rotors for the SORVALL® Pico and Fresco are equipped with biocontainment lids for use with infectious or hazardous samples. The aerosol-tight, Easy-Twist-On lid is exceptionally easy to use. Compact design Create space in your laboratory. Take advantage of the 233 x 335 mm (9 x 13 in) footprint of the little Pico, which can be integrated into any workplace. The low height of the Pico casing makes it easier to load the rotor. The refrigerated Fresco, with a footprint of 290 x 450 mm (11 x 18 in), is smaller than many non-refrigerated centrifuges. Whisper-quiet The Pico and Fresco are valuable aids in the small biological laboratory, where noise can be an issue. Their designs take full advantage of recent discoveries in aerodynamics, and ensure whisper-quiet operation even at maximum speed. High performance The brushless induction motors of the Pico and Fresco are extremely efficient. In 15 seconds or less, they accelerate the microliter rotor to its maximum RCF of 16,000 x g, which is sufficient for all standard applications. A “quick run” key is used for short processing runs.
Stylish, ergonomic design The smooth design of the casing and the modern keypad controller are both easy to clean and pleasing to the eye. The straightforward arrangement of the keys, with icon labels and the EASYcontrol™ microprocessor controller, make operation straightforward. One-touch lid opening allows easy access to the rotor chamber. Ecological refrigeration The refrigerated Fresco uses a CFC-free refrigerant. The powerful refrigeration unit, with standstill cooling, can maintain constant temperatures below 0°C even at maximum speed.
Rotors and accessories for the Pico and Fresco Microliter rotor 75003328 with Easy-Twist-On biocontainment lid Pico
Fresco
Max. capacity
(ml)
24 x 1.5/2.0
24 x 1.5/2.0
Max. speed
(rpm)
13,000
13,000
Max. RCF
(x g)
16,060
16,060
Acceleration time
(s)
15
15
Braking time
(s)
12
16
Max. radius
(mm)
85
85
Reducer sleeves Microliter rotor: adapter sets enable centrifugation of all commercially available microliter and PCR tubes.
Catalog number
24 reducer sleeves for 0.6/0.5 ml microtubes
76003758
24 reducer sleeves for 0.4/0.25 ml microtubes
76003759
24 reducer sleeves for 0.2 ml microtubes
76003750
PCR strip rotor 75003327 with Easy-Twist-On biocontainment lid Pico Fresco
NEW
PCR strip rotor: spin 4 PCR strips (8 tubes each) or 32 individual PCR tubes.
Max. capacity
(4) PCR strips or
(4) PCR strips or
(32) 0.2 ml PCR tubes
(32) 0.2 ml PCR tubes 13,000
Max. speed
(rpm)
13,000
Max. RCF
(x g)
12,846
12,846
Acceleration time
(s)
15
12
Braking time
(s)
15
16
Max. radius
(mm)
68
68
Heraeus Pico Fresco Brochure
18/11/02
11:59 am
Page 2
Specifications SORVALL® Pico Max. RCF (x g) Max. speed (rpm) Min. speed (rpm) Control Drive Noise level at max. speed
SORVALL Fresco
16,060
16,060
13,000 adjustable in 100 rpm increments
13,000 adjustable in 100 rpm increments
2,000
2,000
Microprocessor-controlled via EASYcontrol
™
Microprocessor-controlled via EASYcontrol™
Frequency-controlled brushless induction drive
Frequency-controlled brushless induction drive
< 55 dB (A)
< 55 dB (A) standstill cooling < 45 dB (A)
Run time Program memory Safety features Temperature range Min. temp. at max. speed Dimensions (H x W x D) Weight (without rotor) Power consumption
1 - 99 min, continuous operation, quick run
1 - 99 min, continuous operation, quick run
The most recently entered data remains in the memory
The most recently entered data remains in the memory
Lid locks and interlocks
Lid locks and interlocks
-
-9 to +40°C
-
0°C (at room temp. = 25°C)
8.1 x 9.2 x 13.2 in (206 x 233 x 335 mm)
12.0 x 11.4 x 17.7 in (305 x 290 x 450 mm)
21.3 lb (9.7 kg)
59.4 lb (27 kg)
150 W
270 W
Catalog Numbers SORVALL Pico
includes 24-place microliter rotor 75003328 with Easy-Twist-On biocontainment lid includes 24-place microliter rotor 75003328 with Easy-Twist-On biocontainment lid
SORVALL Fresco
110-120 V/60 Hz
230 V/50-60 Hz
75003236
75003235
75005522
75005521
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[email protected] Latin America Kendro Laboratory Products International Sales · Newtown, CT · USA · Tel. +1 (203) 270-2080 · Fax +1 (203) 270-2210 ·
[email protected] Internet http://www.kendro.com
SORVALL
®
®
Quality Products – Lifetime Care Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards ©2002 Kendro Laboratory Products. All Rights Reserved.
Printed in U.S.A. (11/02) S00239
Maintenance
2 Service 2.1 Servicing Schedule (yearly procedure recommended) 2.1.1 Maintenance Routine without Dismantling the Centrifuge 2.1.1.1 Electrical Installation and Safety · switch OFF the centrifuge and disconnect the unit from power, check voltage supply and mains fusing (15 Amps, slow blow characteristic) · check condition of plug and wall socket - (let) replace defective parts · check cord condition and fixing / connection - replace or refit it · check condition of instrument socket and replace it in case of bad contacts 2.1.1.2 Location and Mechanical Installation · check the base (ground, table, lorry with lockable wheels etc.) for resonance-free and stable conditions · check for a well ventilated place and sufficient distances to walls or adjacent equipment, without exposition to direct sunlight · check the leveling of the centrifuge drive with use of a spirit level 2.1.1.3 Lid Locking Mechanism and Safety Device · connect the centrifuge to power and switch ON · check for easy lid closing and self-acting lid opening - if in disorder, readjust lid's swivel hinge and/or torsion spring · check the central rubber gasket for lid sealing and replace it, if damaged · for checking of the safety circuit: start the centrifuge let it shortly run and stop it, the lid must not be opened as long as the message "end" will be indicated - if safety circuit is out of function, replace main board 2.1.1.4 Cleanliness of Spin Chamber and Motor Casing · open the lid and remove the rotor (for loosening turn socket wrench in arrow direction - see rim of rotor chamber, push 2 screw drivers in opposite holes and pull the rotor vertically out) · clean the spin chamber with a dry and absorbent cloth (remove all dust and moisture - see also section for Cleaning) · check the correct seat and condition of the motor cover and take care of the cleanliness of the annular slot around the motor shaft: penetrating fluids can damage the upper motor bearing, remove fluids with an injector and/or absorbent paper 2.1.1.5 Rotor and Accessories Condition and Sealing · check the condition of rotor and accessory parts (especially all supporting or stressed partitions): the rotor and/or accessory parts must not be used any longer, if there are visible traces of mechanical damage or rust · check the condition of rotor and/or accessory sealing and replace them in case of malfunction 2.1.1.6 Rotor and Accessories Condition and Sealing · check the condition of rotor and accessory parts (especially all supporting or stressed partitions): the rotor and/or accessory parts must not be used any longer, if there are visible traces of mechanical damage or rust · check the condition of rotor and/or accessory sealing and replace them in case of malfunction 2.1.1.6 Rotor Fixing and Motor Shaft · check the trouble-free condition of the locking nut and replace it in case of malfunction · check the condition of the drive motor shaft: the centrifuge must not be used any longer, if the drive shaft is damaged (bend, thread is worn out, etc.) 2.1.1.7 Temperature Control Circuit · clean the condenser with a dust exhauster · check the thermal isolated fixing and the correct place of the temperature sensor · reinstall the rotor and run the centrifuge at maximum rotor speed and at 4°C, check the temperature 15 minutes later: the displayed and measured value should be approximately the same (allowed tolerances ±2°)
Edition: 01 17.11.02 MH
2-1
BIOFUGE fresco
Maintenance
2.1.2 Maintenance Routine after Dismantling the Centrifuge Casing 2.1.2.1 Motor Supporting Elements · check the supporting and damping motor elements and replace them in case of increased rubber abrasion or abundance of imbalance but at least every 3 years 2.1.2.2 Braking Circuit · check the function of the braking circuit (warming up of brake resistor, even and noiseless brake effect) and replace defective parts in case of malfunction 2.1.2.3 Ventilation and proper Air Flow · check the sucking performance of the fans (a piece of paper must be sucked on), if necessary clean or replace defective fan · check the air path and lateral sealing: no air must be redrawn from inside, if necessary replace the lateral insulation 2.1.2.4 Lead and Screwing Connections · check the terminal and plug connections of all leads and on all boards and electrical components, tighten all loosen screwing connections, refit or replace defective parts · check the screwing connections of all boards, mechanical and electrical components and retighten them if necessary (use screw locking lacquer for motor mounts and lid lock assembly) 2.1.2.5 Protection Earth Core and Grounding Connections · check the protection earth core for continuity and all grounding plug connectors · check isolation resistance and accessible current
Edition: 01 17.11.02 MH
2-2
BIOFUGE fresco
Maintenance
2.2
Trouble Shooting Error Error Cause Indication
no mains voltage supply displays remain dark
no low voltage supply for indication board interrupted program
all display elements are shortly illuminated constant
centrifuge shakes badly
CPU program reset may be caused by EMI Program interruption
imbalance run
Possible Error Corrective Procedure Source mains fuse or circuit check fuse or circuit breaker and replace or switch on breaker failed again defective mains cord, check leads, socket and mains switch, replace socket or mains defective parts switch unit fuse or fuses on Replace the fuse, if fuse blows up again, search for main board defective other faults faulty connection check connections on main and key board, check from main board to connecting leads, replace faulty parts key board faulty indication or replace main board completely main board NV-RAM out of insert the valid NV-RAM and push it correctly into socket or not socket correctly placed reduced voltage remedy the failure supply (<95V) if the voltage drops often, use a voltage stabiliser bad or missing check all ground connections and the ground ground connection connection of the main boards parameter NV-RAM rotor not symmetrically loaded base is not sturdy enough and comes into vibrations centrifuge is not correctly leveled
level the centrifuge correctly, use a spirit level on top of centrifuge
rotor has imbalance (mechanical causes)
rotor must not be used any longer, send back to factory
drive shaft is bent, rotor fixing is damaged missing mains voltage lid coil is not or PTC resistor has not sufficiently lid cannot be released supplied with opened by voltage faulty driving or triac key circuit pressure at faulty lid coil faulty winding of coil standstill lid bolt is jamming lid is not lid is deformed or correctly locked disadjusted
Edition: 01 17.11.02 MH
no or defective NV-RAM
2-3
open lid, check load, close lid again and start change or reinforce the base (floor, table lorry with lockable wheels)
replace the drive motor remedy see above, opening device only at standstill after a waiting time of 1-2 minutes press key again replace complete main board replace complete lid lock push lid centrically into lock and press the key again re-adjust the lid centrically
BIOFUGE fresco
Maintenance
Error Indication
Error Cause Lid was opened manually during run
„LId“ message appears in speed display
protection circuit (15V) for lid and motor temperature control was interrupted during run
„OPEn“ message in display
15V supply circuit is interrupted at standstill
“br“ message appears in speed display
rotor comes to standstill without braking force rotor didn't turn
motor didn't start
E-04 flashes
faulty temperature measuring test: PT100 substitution by resistors, indication after power ON
Edition: 01 17.11.02 MH
check leads and micro switch
motor overtemperature switch short interruption of mains supply bad contact of instrument supply line
check motor over-temp. switch and leads for continuity, replace faulty parts
rotor is jammed
defective drive faulty main board faulty temperature sensor (PT100) or leads defective amplifier or converter
compressor didn't chamber over work temperature indication value >50°C loss of refrigerant chamber under temperature indication <-10°C
E-08 flashes
micro switch
motor is jammed connection: drive main board
E-00 flashes
E-04 flashes
Trouble Shooting Possible Error Corrective Procedure Source Forbidden! close lid immediately, turn power OFF/ON, wait for Lid opening device must only be used at termination of br phase until end message appears standstill check leads and connectors to micro switch, in case Defective micro of a faulty micro switch, replace lid lock device switch or leads or completely connectors to micro switch are let motor cool down, then check temperature switch interrupted and leads with Ohmmeter Motor overcheck air cooling, circulation temperature switch has tripped (no check solderless connections XM1-3 and motor sufficient air flow or windings, replace defective parts motor has run on 2 phases only)
Excess voltage of DC intermediate circuit
Wait for rotor standstill (approx. 75 sec) and re-start Check plug contacts of supply line and replace defective parts if necessary check for easy movement, remove any jamming objects remove drive, replace faulty parts check terminal and lead connections, replace faulty parts check resistance of motor windings, replace faulty parts replace main board check plug connection and resistance of PT 100, replace faulty sensor set the temperature to 20°C and watch the display: 100W ® (0±2)°C 120W ® (50±2)°C if not replace the main board check leads and voltage supply on main board and compressor, replace defective parts search for leakage, evacuate and refill with new refrigerant
compressor didn't cut off
check compressor relay and relay driving, replace defective parts
voltage pikes on mains supply
if happened repeatedly, connect a surge inter mains and unit
faulty brake path
check leads and brake resistor, replace defective parts
faulty component
replace main board
2-4
BIOFUGE fresco
Maintenance
Error Indication E-10 flashes
E-11 flashes
E-23 flashes
Edition: 01 17.11.02 MH
Error Cause
Trouble Shooting Possible Error Source
checksum error NV-RAM wrong or not of NV-RAM initialised
error during data exchange from NV-RAM
too much tolerances during temperature calibration
Corrective Procedure insert the correct NV-RAM
sporadic: strong electromagnetic disturbance
if error returned very often screen the disturbance source or operate the centrifuge on another place
continuously: error on main board
replace defective main board
sporadic: strong electromagnetic disturbance
if error returned very often screen the disturbance source or operate the centrifuge on another place
continuously: error on main board
replace defective main board
2-5
BIOFUGE fresco
Maintenance
2.3
Test Points Test Points Unit Value
Conditions all given values are related on 120V (±10%) mains voltage for board’s supply
mains terminal XN
120V AC
terminal XM motor voltage values must be the same between all 3 motor leads (U,V,W)
in each case measured inter 2 motor leads after reaching selected speed no effective values! 115V AC 3000min-1 , rotor #3327, #3328 155V AC 6000min-1 210V AC 13000min-1 acc. time: 15s, dec. time: 17s
motor current IM cable (U,V,W)
soft iron or digital effective measuring meter(true RMS) Max. during acceleration (#3327, #3328) 3 * 0,6A rotor #3327, #3328 -1 speed = 13000min 320V DC at standstill 350V DC max. during braking phase Bremsens (#3327, #3328) rotor #3327, #3328 305V DC -1 speed = 13000min
intermediate voltage UD inter U+ (cathode D4) and U- (anode D5) 275V DC shortly at maximum acceleration (#3327, #3328) current ID of intermediate circuit, (measured as voltage drop across control resistor R100) UM = ID * RM
-1
25mV DC 3000min , Rotor #3327, #3328 -1 40mV DC 6000min -1 65mV DC 13000min 180mV DC shortly at maximum acceleration (#3327, #3328) -45mV DC shortly at maximum braking (#3327, #3328)
motor winding resistance 20°C
3 x 14W
-insulation value
> 10MW
brake resistor R24 parallel R101 compressor terminal XG terminal box pull off starting relay -insulation
235W 3,6W 5,5W 10,4W 0,3W > 10MW
switch OFF unit, pull off motor plugs, measure inter 1 -> 2, 2 -> 3, 3 -> 1 resistance inter each phase and motor casing resistor temperature: 20°C compressor temperature 20°C resistance main coil (between terminal 13 + 14) auxiliary coil (between terminal 12 + 13) coil starting relay inter phase and compressor casing
fan motor terminal XK
150W
resistance disconnect one pole of terminal XK fan temperature20°C
lid solenoid terminal XC
20W
resistance , not activated
lid switches plug XA
15V DC
voltage drop by open lid
Over temperature switch Motor terminal XB
15V DC
voltage drop by closed lid and disconnect one pole of terminal XB
Temp. Sensor plug XU
95mV
voltage drop chamber temperature 20°C (PT100: » 108W)
Edition: 01 17.11.02 MH
2-6
BIOFUGE fresco
Maintenance
2.4
Mounting and adjustment of the torsion spring
2.4.1 Purpose The torsion spring offers the user a comfortable support when closing and opening the centrifuge lid. When the "open lid" key is pressed, the lid lock is released on both sides and the lid bursts open and remains in a 20° position. Up to an angle of 45° the lid remains stable in any chosen position, beyond 45° it automatically opens to full extent. 2.4.2 Mounting The torsion spring assembly contains the following parts: · torsion spring · 2 plain bearing bushes (for shaft guidance) · 2 bushing nipples (for leg catch) · 2 set screws (aid for fixing legs) · dowel pin (for hooking the torsion spring onto the shaft) · tube of Loctite 221 (for fastening screws) · tube of Molykote TP42 (for lubrication of the torsion spring) Preparation: The defective torsion spring has been removed as described in Section 5, the old plain bearing bushes and bushing nipples have been removed. 1. Press the new plain bearing bush from outside into the right-hand bore of the chassis. 2. Slip on both bushing nipples up to the head of the hexagon bolt M6x12. Fix the screws again to the chassis and secure with Loctite 221. 3. Push the old dowel pin carefully from the shaft using a pin punch, then push the new dowel pin on using a plane surface (e.g. a wooden panel). Mounting the shaft and torsion spring: 4. Grease the short end of the shaft evenly using about 5 g of Molykote TP42. Attention! Do not cover the plain bearing with grease! 5. Slip the torsion spring onto the short end of the shaft (short loop first). 6. Push the long end of the shaft through the left-hand bore of the chassis and slip into right-hand plain bearing bush. 7. Press the plain bearing bush via the shaft into the left-hand bore of the chassis. 2.4.3 Adjustment Preassembly and alignment of the legs: 8. Push both legs onto the ends of the shaft such that the bores are horizontally oriented and the leg ends point forward. 9. Screw the M8 nuts loosely onto each of the shaft ends to position the legs. 10. Screw one dowel pin each tightly into the rear threaded bores, thereby fixing them with the bore in the chassis. Stretching the torsion spring: 11. Push a suitable screwdriver from behind into the left-hand bore of the shaft and turn totally to the front. Grip the shaft via the middle bore with another screwdriver and turn it to approximately 15° from horizontal. 12. Tighten fast both nuts of the shaft. 2.4.4 Assembling lid and housing 13. Put lid in place. It must catch audibly into both locks. 14. Screw on front cap screws on both sides, thereby fastening the lid tightly to the two legs. 15. Replace one by one the two set screws in the rear bores by the remaining two cap screws. 16. Manually release the locks. The lid should come to rest at an angle of 15-30°. If this is not the case, restretch using the middle bore of the shaft. Should this be impossible, the lid must be disassembled and the spring must be stretched anew! 17. If the angle is okay, secure the M8 nuts with one drop of Loctite each on the legs. 18. Close the lid and replace the rear cap screws on both sides by set screws. Attention! Set screws must not jut out of the legs!
Edition: 01 17.11.02 MH
2-7
BIOFUGE fresco
Maintenance
19. Screw lid off and mount housing. 20. Reassemble lid as described above and check opening angle of 15-30°. 2.5 Assembly and Adjustment of the Drive 2.5.1 Purpose of the adjustment The temperature measurement of the Biofuge fresco is done using a slim temperature probe. The tip of this temperature probe is fixed at a very short distance to the motor cover as well as to the rotor. In order to guarantee an accurate temperature measurement, a defined distance to both rotor and motor cover is mandatory! For this purpose, the drive must be adjusted. 2.5.2 Mounting / Adjustment of the drive with help of the rotor #3327/3328 Fasten rubber buffers to the transverse crosshead using nuts and washers. · Place transverse crosshead inside the chassis and fasten with screws such that the screws are aligned with the upper ends of the long holes. · Connect the motor to the rubber buffers using the screws and locking rings. Adjusting the transverse crosshead · Put three cylindrical spacers with a thickness of 6,0±0,1 mm (diameter 10 to 20mm) for rotor #3327/3328 into the evaporator. · Position the rotor onto the motor shaft and fasten with flanged nut. · Loosen the screws about ½ turn and carefully move transverse crosshead downward until the rotor rests on top of the spacers. Attention! Make sure the transverse crosshead is horizontally placed. Besides, the rubber buffers must not be stretched or tilted. · Fasten all 8 screws of the transverse crosshead. Checking the adjustment · Replace the spacers by three 5,0±0,1 mm thick spacers (diameter 10 to 20mm) for rotor #3327/3328. Fasten rotor again as prescribed. · Turn rotor manually. The motor is correctly mounted if the rotor does not touch the spacers. If it does, the adjustment must be repeated. · Remove spacers and secure screws with a drop of screw lock. · Reinstall centrifuge and rotor as prescribed and do a test run. 2.6
Cleaning of Instrument Parts
ATTENTION - WARNING! The electrical and electronic components must not be cleaned with moist detergents! For Cleaning the centrifuge housing or its accessories see Operating Instructions section 1 (maintenance and care). · Electronic components Clean dusty components carefully with a dry and soft brush and remove loose dust with a vacuum cleaner. · Fans Scratch off carefully with a knife or similar tool the crusted dirt from the fans’ blades. Resulting grooves or marks must be removed subsequently with a fine abrasive cloth. Loose dirt is to remove with an absorbent cloth or vacuum cleaner. · Vent holes Remove dirt from the vent grid behind the front panel by using a brush and vacuum cleaner. · Condensers of cooling plant The louvers between the condensers’ heat air deflectors have to be vacuum cleaned. In case of persistent dirt the unit must be carried to an appropriate place where the condensers can be cleaned with compressed air from inside to outside. Bent heat air deflectors have to be realigned correctly.
Edition: 01 17.11.02 MH
2-8
BIOFUGE fresco
Maintenance
2.7
Electrical Safety Check
ATTENTION! A final electrical safety check must be performed after each maintenance and/or repair! · · ·
Resistance check of protective conductor The measuring value of the resistance between the mains plug's grounding pin and the grounding conductors of the motor, electronic chassis and the casing must not exceed 200 mW. Insulation resistance Check Check also the insulation resistance between the poles of the mains plug and the grounding conductor; the resistance value must be more than 2 MW. Accessible current measured to EN 61 010 The accessible current must not exceed 3.5 mAmps in single fault condition (interrupted protection earth wire)! In accordance with the EN61010, IEC1010 and UL3101 such a fault condition can be reproduced by the following measuring circuit.
Steckergehäuse / plug-in casing
0,22µF ±5%
0,022µF ±5%
N (L)
Körperstrom: accessible current: I [mA] = U [mV] / 500:
PE
Edition: 01 17.11.02 MH
V AC Specifaction for the meter - TRMS, DC - 5kHz or more - Input resistance > 1MW - Tolerance 5% or better - Crest Factor 5 or better
500W ±1%
L (N)
Spezifaktionen für Meßgerät - TRMS, DC - 5kHz oder mehr - Eingangswiderstand > 1MW - Toleranz 5% oder besser - Crest Faktor 5 oder besser
10kW ±5%
Umax = 1750mV º Imax = 3,5mA
2-9
BIOFUGE fresco
FUNCTIONAL DESCRIPTION
3
Functional Description
3.1
Block Functions
The BIOFUGE fresco is a microprocessor controlled laboratory tabletop centrifuge with induction drive motor and refrigerator. Following boards and components are located inside of the unit (see block diagram section 4 page 4-1): · Two poles mains switch is mounted in the bottom of the front panel's right side · Main board with microprocessor part and power electronics, 2 fuses (4AT) are serving for board protection · Key and indication board (EASYCONTROL programming with temperature display) · Three phase induction drive motor with an integrated thermal over temperature switch (cut-out at 140°C) · Temperature sensor PT100 · Compressor with starting relay (inside terminal box) and starting capacitor · Two lid lock assemblies with solenoid and integrated micro switches (mechanical bolt keeper, magnetically dislocking) · Brake resistor mounted on main board · Two fans (parallel connected) for cooling of condenser, drive, compressor and electronics 3.2
Main Board Functions
The main board is mounted on the rear side of the front panel. The components on main board are arranged in following groups (see also wiring diagram page 4-2 and 4-3). · Board fusing (2 x 4 Amps. slow blow feature) · Noise Filter for suppression of radio interference, caused by the fast switching power electronics (9,9 kHz), in accordance with the German Regulation VDE 871, grade B · Power pack for low voltage supply of microprocessor part (potential separated by transformer) and power electronics (mains potential) · Triac control circuit for both parallel connected fans · Triac control circuit for both parallel connected lid solenoids ( DC supply via bridge rectifier) · Triac control circuit for compressor relay · Bridge rectifier for DC intermediate circuit supplying brake control path and frequency converter · Microprocessor part with Controller (central processing unit) and ASIC80 (specific integrated circuit for controlling of centrifuge functions)
Edition:01 28.11.02 MH
3-1
Biofuge fresco
FUNCTIONAL DESCRIPTION
3.2.1 Power Pack The power pack consists of a transformer, bridge rectifier and voltage regulator. · U1 = 5 V supplies central processor, key and indication board and temperature measuring circuit reference potential A1: connected to protective conductor (GND) Additional generated by one way rectifying from the mains voltage: · U2 = 15 V supplies control electronics of frequency converter via the protection circuit of FET over temperature switch (118°C - normal closed), closed lid switch and motor over temperature switch (normal closed) reference potential: A2! mains potential! EXERCISE CAUTION when measuring etc. 3.2.2 Intermediate Circuit with Brake Path and Frequency Converter Intermediate Circuit The DC intermediate circuit serves as an energy store between the AC power input and the transmitted motor performance. The intermediate circuit consists of a bridge rectifier (4 diodes) and one reservoir capacitor which is softly charged, continuously supplied during operation and slowly discharged after power off. Brake Path Electrical power is fed back into the intermediate circuit during motor deceleration (motor acts as generator). This braking power is transformed into heat by two big brake resistors (parallel connected) on board so that the intermediate circuit voltage does not rise to an excessive level (see section 2 test points on main board). The brake resistors are switched into the intermediate circuit by a fast switching transistor (pulse width modulation). This transistor is voltage dependant controlled by a selfacting stage (closed loop). Frequency Converter The motor is 3-phased provided with chopped direct voltage blocks. These blocks are variable in frequency and pulse width modulation and are dephased to 120° between the motor windings. Beginning with low frequency and small pulse width chopped blocks, both parameters will be increased up to the operators set speed. By this variation the acceleration power of the drive motor can be effected. For deceleration the frequency and pulse width parameters will be reduced. The frequency converter is protected against over current, over temperature and over voltage.
Edition:01 28.11.02 MH
3-2
Biofuge fresco
FUNCTIONAL DESCRIPTION
3.2.3 Microcontroller (Central Processing Unit) The controller block includes the central processor unit (CPU) 80C21 PLCC and the data storage (NVRAM) 9346 with the software identification No's. for: · CPU:
097 8 xx (first displayed No. in speed, time and temperature field)
· RAM:
415 8 xx (second displayed No. in speed, time and temperature field)
The second parts of these No.'s (xx displayed in the temperature field) will be upgraded if necessary. The ROM with control program is integrated in the CPU. The most important operating parameters (e.g. the maximum data or the last operator settings) are stored in the non volatile (NV) -RAM. The CPU co-operates with the ASIC 80 component via the 8 bit data bus and some control lines. The ASIC 80 is a specially designed integrated circuit for the driving of our centrifuges (with induction motors), containing additional functions for temperature measuring and electronics protection.
Edition:01 28.11.02 MH
3-3
Biofuge fresco
FUNCTIONAL DESCRIPTION
3.3 Key and Indication Board The key and indication board is mounted behind the display. The connection to the main board is done by a 14 pole flat cable. The 7-segment displays, the control key LED and the operating keys are also managed by the CPU. For the programming serves EASYCONTROL (see Operating Instruction Manual). Function of key elements keys S1 S2 S3 S4 S5 S6 S7 S8 S9 S10
name lid symbol double arrow square symbol arrow to right speed arrow down speed arrow up temp. arrow down temp arrow up time arrow down time arrow up
function(s) to open the lid (only possible at standstill) quick run, to start - keep the key pressed stop, to terminate the centrifugal run manually start, to set a centrifugal run in rotation to decrease the value of the set speed to increase the value of the set speed to decrease the value of the set temperature to increase the value of the set temperature to decrease the value of the set time to increase the value of the set time
Function of indicating elements indicat. H01,H02
name temperature range
H03,H04
time range
H5 – H7
speed range
H8, H9, H12, H13
rotation-LED’s
Edition:01 28.11.02 MH
function(s) indication of sample temperature, right part of software identification numbers of CPU and NV-RAM indication of centrifugation time and hold (hd), left part of software identification numbers of CPU and NV-RAM -1 indication of speed [·1000 (min )] · left part of software identification number · run finished (“End“) · error messages like (“LId“, “E- “) indication of rotation after starting till “End“
3-4
Biofuge fresco
SCHEMATIC DIAGRAMS For units with 120V mains voltage instrument plug x1000
BIOFUGE
S1 mains switch
key and indication board
XF
XF XN
main board brake resistor
XA
XB
XC
CPU
XM
NV-RAM
XK
XU
XG
F3
Y1
S3 S1
Y2
3~
S4 S2
V1
PT 100
M1 lid solenoid
lid switch
lid lock left side
lid solenoid
lid switch
overtemp. switch
motor
M2 temp. sensor
M3
M4
fan for compressor, condenser
compressor
lid lock right side
legend screwing connection
Edition: 01
Block Diagram
16.12.02 He
4-1
plug connection
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 120V mains voltage
transformer, rectifiers and regulators
main board
5V (VCC)
7805
A1=GND
instrument plug with mains switch
driving of FETs (frequency converter)
+15V 0V=A2
S0
DC intermediate circuit
noise filter
XL
J
F1
1
8AT
NTC
XN
2
XPE
"E-07" 5
K1
"E-08"
J
autom. brake control
PTC
"E-08"
"E-07" R1 220 brake resistor
XG/1
XG/2
XV/1
XB/1
XV/2
XB/2
XA/1
XA/2
XC/1
XC/2
M2
14
"E-17"
10 S1..S4
PE chassis PE motor
PE compressor PE condensor fan
Î M2
"E-07" 11
"LId" CS
M3
13 "E-07" compressor with starting relay
Y1
"E-17"
Y2
lid switch
lid solenoid
3
fan for condenser
4
Edition: 01
Wiring Diagram
16.12.02 He
4-2
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 120V mains voltage S6
key and indication part
H3
H4
H9
H5 H7
EASYCONTROL
H6
U1 (5V) A1
S2
H2
H1
S5
S1
H8
MUX
A,B F,E
3
A,B,C,D E,F,G,DP
S4
S3
S8
S7
8
XF 14
XF
main baord part 2
Y1, Y2
U1 (5V) A1 = GND
RESET
µP part frequency converter
1
U2 (15V)
5
UD+ 13
2x6 HGTPs INHIBIT
ASIC 80
K1(M2)
8
4 NV-RAM
CPU
"E-” “ 0" "LId"
"E-10" "E-11"
i dyn
"OP" Û
2
R44
A2
3
idyn
current control res.
“LId”...
idyn “E-” “ 0”
UD-*
XM/1
15V
“E-” “ 8”
XM/2
"E-” “ 0"
3
F
"E-7"
U
XM/3
physical separation
F3
V1
"LId"
temperature sensor PT100
overtemperature switch
J 4
Edition: 01 16.12.02 He
M1 Motor motor
Wiring Diagram 4-3
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 120V mains voltage
mounting of unit ground, left side in front
mounting of unit ground, right side in front key and indication board 1x PE - terminal 1 pole 1x screw M4x10mm 2x fan washer 4,3mm
PE - fan M2
PE - fan M3 bottom plate
bottom plate 1x PE - terminal 3 pole 1x screw M4x10mm 2x fan washer 4,3mm
heat sink
Y1
XU
a
b
PE - main board
lid solenoids Y1 und Y2
main board
Y2 a
b
amored chamber
1
toroidial core, Valvo 4322 020 97183 + cable tie
XC 2
1
perform a most narrow winding as possible!
S1
4 2
1 fans for condenser M2 und M3
XK M2
M3
2
2
Y1
S3
b a
1
XG
temperature sensor
2
lead-in hole for sensor
M4
compressor
L
F1
pass through from inside all leads marked by ellipse
N
PE - motor (from motor harness)
brake resistor R 101
XN XB1
XB2
XA
XM1
XM2
XM3
b a
PE
lid lock, right side
1 S4
Y2
5 mains switch S0
4
2
1 I
mounting mains ground
motor overtemp. switch XB1 u. XB2
PE - compressor
1
no. 1
no. 2
no. 3 2 4
4
0
1
N
L
S2 PE - armored chamber foot, right side in the rear
1
PE 1x screw M4x25mm 1x PE - terminal 3 pole 6x fan washer 4,3mm 3x nuts M4
4 2
S1
lid switches S1 u. S2 instrument plug
4
lid lock, left side 1
S2 4
M1 PE - motor drive motor
1x screw M4x16mm 2x fan washer 4,3mm 1x nut M4
PE - mains ground PE - armored housing
Edition: 01
Wiring Connections Diagram
16.12.02 He
4-4
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 120V mains voltage compressor terminal box
drive motor wiring
starting relay
starting capacitor terminal: 11 u. 13 mains - L shrinkage hose 12,7mm + 1 cable tie SST1 11
starting capacitor
10
120mm mains - N
13
12
5x 400 mm
14
PE terminal box XB1 PE
compressor wiring
overtemp. switch
XB2 XM1 XM2
+
XG/N
motor connections
XM3
+
XG/L
PE - motor, 300mm to amored chamber in front
PE - compressor to mains ground
XK/L
fans
wiring fans
XK/N
PE - Lüfter PE - fans
M2
M3 PE - main board
unit ground, left side in front
1x PE - terminal 1 pole 1x screw M4x10mm 2x fan washer 4,3mm
1x PE - terminal 1 pole 1x screw M4x10mm 2x fan washer 4,3mm
bottom plate
Edition: 01
Wiring Connections Diagram
16.12.02 He
4-5
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 120V mains voltage
125 mm
XB1/2 overtemperatur switch
140 mm
XM1/3 motor, connect. 1, 2, 3
140 mm
XC
lid solenoid’s Y1 + Y2
150 mm
XG
compressor
300 mm
XK
fans M2 + M3
210 mm
clip 6,5mm front of unit clip 6,5mm
length from clip
overtemp. switch
designation lid switches S1 + S2
drive
XA
adhesive clip
free cable’s length up to first fixing clip
fan 2, left side
detail view of fans cable conduction by clips
KM
plug
fan 3, right side
unit socket
*
coil switch
PE - fan 2 fan M2
PE-fan 3 *
*
fan M3
S0
temperature sensor
cable conduction unit
lid lock, switches
drive
fans
lid lock, coil
compressor
mains input
overtemp. switch
mains switch
Edition: 01
Wiring Connections Diagram
16.12.02 He
4-6
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 100V mains voltage instrument plug x1000
BIOFUGE
S1 mains switch
key and indication board
auto transformer 100/115V
XF
100V
XF 115V
XN
main board brake resistor
XA
XB
XC
CPU
XM
NV-RAM
XK
XU
XG
F3
Y1
S3 S1
Y2
3~
S4 S2
V1
PT 100
M1 lid solenoid
lid switch
lid lock left side
lid solenoid
lid switch
overtemp. switch
motor
M2 temp. sensor
M3
M4
fan for compressor, condenser
compressor
lid lock right side
legend screwing connection
Edition: 01
Block Diagram
16.12.02 He
4-7
plug connection
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 100V mains voltage
transformer, rectifiers and regulators
main board
5V (VCC)
7805
A1=GND
instrument plug with mains switch S0
driving of FETs (frequency converter)
+15V
auto transformer 100/115V
0V=A2
noise filter
XL
DC intermediate circuit J
F1
1
8AT
T0
NTC
XN
2
XPE
"E-07" 5
K1
"E-08"
J
autom. brake control
PTC
"E-08"
"E-07" R1 220 brake resistor
XG/1
XG/2
XV/1
XB/1
XV/2
XB/2
XA/1
XA/2
XC/1
XC/2
M2
14
"E-17"
10 S1..S4
PE chassis PE motor
PE compressor PE condensor fan
Î M2
"E-07" 11
"LId" CS
M3
13 "E-07" compressor with starting relay
Y1
"E-17"
Y2
lid switch
lid solenoid
3
fan for condenser
4
Edition: 01
Wiring Diagram
16.12.02 He
4-8
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 100V mains voltage S6
key and indication part
H3
H4
H9
H5 H7
EASYCONTROL
H6
U1 (5V) A1
S2
H2
H1
S5
S1
H8
MUX
A,B F,E
3
A,B,C,D E,F,G,DP
S4
S3
S8
S7
8
XF 14
XF
main baord part 2
Y1, Y2
U1 (5V) A1 = GND
RESET
µP part frequency converter
1
U2 (15V)
5
UD+ 13
2x6 HGTPs INHIBIT
ASIC 80
K1(M2)
8
4 NV-RAM
CPU
"E-” “ 0" "LId"
"E-10" "E-11"
i dyn
"OP" Û
2
R44
A2
3
idyn
current control res.
“LId”...
idyn “E-” “ 0”
UD-*
XM/1
15V
“E-” “ 8”
XM/2
"E-” “ 0"
3
F
"E-7"
U
XM/3
physical separation
F3
V1
"LId"
temperature sensor PT100
overtemperature switch
J 4
Edition: 01 16.12.02 He
M1 Motor motor
Wiring Diagram 4-9
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 100V mains voltage
mounting of unit ground, left side in front
mounting of unit ground, right side in front key and indication board 1x PE - terminal 1 pole 1x screw M4x10mm 2x fan washer 4,3mm
PE - fan M2
PE - fan M3 bottom plate
bottom plate 1x PE - terminal 3 pole 1x screw M4x10mm 2x fan washer 4,3mm
heat sink
Y1
XU
a
b
PE - main board
lid solenoids Y1 und Y2
main board
Y2 a
b
amored chamber
1
toroidial core, Valvo 4322 020 97183 + cable tie
XC 2
1
perform a most narrow winding as possible!
S1
4 2
1 fans for condenser M2 und M3
XK M2
M3
2
2
Y1
S3
b a
1
XG
temperature sensor
2
4
lid lock, left side 1
lead-in hole for sensor
M4
compressor
L
F1
pass through from inside all leads marked by ellipse
N
blue 100V
auto transformer T0
black 115V
PE - motor (from motor harness)
brake resistor R 101
XN XB1
XB2
XA
XM1
XM2
XM3
b a
PE
lid lock, right side
1 4
S4 red
red
5 mains switch S0
Y2
4
2
1 I
mounting mains ground
motor overtemp. switch XB1 u. XB2
PE - compressor
1
no. 1
no. 2
no. 3
S1
2 4
4
0
1
lid switches S1 u. S2 instrument plug
N
L
S2 PE - armored chamber foot, right side in the rear
1
PE 1x screw M4x25mm 1x PE - terminal 3 pole 6x fan washer 4,3mm 3x nuts M4
2
S2 4
M1 PE - motor drive motor
1x screw M4x16mm 2x fan washer 4,3mm 1x nut M4
PE - mains ground PE - armored housing
Edition: 01
Wiring Connections Diagram
16.12.02 He
4 - 10
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 100V mains voltage compressor terminal box
drive motor wiring
starting relay
starting capacitor terminal: 11 u. 13 mains - L shrinkage hose 12,7mm + 1 cable tie SST1 11
starting capacitor
10
120mm mains - N
13
12
5x 400 mm
14
PE terminal box XB1 PE
compressor wiring
overtemp. switch
XB2 XM1 XM2
+
XG/N
motor connections
XM3
+
XG/L
PE - motor, 300mm to amored chamber in front
PE - compressor to mains ground
XK/L
fans
wiring fans
XK/N
PE - Lüfter PE - fans
M2
M3 PE - main board
unit ground, left side in front
1x PE - terminal 1 pole 1x screw M4x10mm 2x fan washer 4,3mm
1x PE - terminal 1 pole 1x screw M4x10mm 2x fan washer 4,3mm
bottom plate
Edition: 01
Wiring Connections Diagram
16.12.02 He
4 - 11
BIOFUGE fresco
SCHEMATIC DIAGRAMS For units with 100V mains voltage
auto transformer BV30326 Prim.100V Sek.115V
125 mm 140 mm
XM1/3 motor, connect. 1, 2, 3
140 mm
XC
lid solenoid’s Y1 + Y2
150 mm
XG
compressor
300 mm
XK
fans M2 + M3
210 mm
coil switch
PE - fan 2 fan M2
clip 6,5mm front of unit
*
auto transformer 100/115V
XB1/2 overtemperatur switch
clip 6,5mm
length from clip
overtemp. switch
designation lid switches S1 + S2
drive
XA
adhesive clip
free cable’s length up to first fixing clip
fan 2, left side
detail view of fans cable conduction by clips
KM
plug
fan 3, right side
unit socket
PE-fan 3 *
*
fan M3
S0
temperature sensor
cable conduction unit
lid lock, switches
drive
fans
lid lock, coil
compressor
mains input
overtemp. switch
mains switch
Edition: 01
Wiring Connections Diagram
16.12.02 He
4 - 12
BIOFUGE fresco
SCHEMATIC DIAGRAMS
XU Stecker Temperaturfühler plug temperature sensor XD Anschluss Service plug service
XC Anschluss Deckelspule terminal lid solenoid XK Anschluss Lüfter terminal fans
XG Anschluss Kühlmaschine terminal compressor
XN Anschluss Netz terminal mains
XA Stecker Deckelschalter plug lid lock, switches
XB1, Xb2 Anschluss Motorübertemp. terminal overtemp. switch
XM1-XM3 Anschluss Motor terminal drive motor
Edition: 01
Component Plan Main Board
16.12.02 He
4 - 13
BIOFUGE fresco
SCHEMATIC DIAGRAMS
Controller
power electronic INHIBIT PHASE1_O PHASE1_U PHASE2_O PHASE2_U PHASE3_O PHASE3_U
INHIBIT PHASE1_O PHASE1_U PHASE2_O PHASE2_U PHASE3_O PHASE3_U protection circuit
+15
+15
U-*
U-* U+
UEBERSTROM /UEBERSPANNUNG
TEMPERATUR
DECKELAUF
TEMPERATUR
TEMP_0
TEMP_0
TEMP_1
TEMP_1
U+ ZK2
U+ ZK2
U0
U0
U-
U-
page 4-17 DECKELSCHLOSS KUEHLUNG LUEFTER
page 4-15
power supply
/UEBERSPANNUNG
temperature measurement DECKELAUF
page 4-19
UEBERSTROM
page 4-16
DECKELSCHLOSS KUEHLUNG LUEFTER page 4-15
Edition: 01
Wiring Diagram Main Board
16.12.02 He
4 - 14
BIOFUGE fresco
SCHEMATIC DIAGRAMS R16 2R5 NTC
EMI Filter Funkentstoerfilter
Power Connection XN
F1
L1
1
L
1 2
N
SI 8AT 6.3x32 UL
3
2
R18
1N4007
470R SMD
ZK2
D4
BR1 1
C15 0u6 X
R17 120k
D9 Hilfszwischenkreis
C16 2n2 Y
P600K
4
C19 220n 400V
XPE1 PE
2x5.6mH 2A
2
R14 56k
U+
BR_POWER
C13 470u 200V
U0
DC intermediate circuit
C14 470u 200V
R15 56k D5 C9 2n2 Y
P600K
U-
C10 2n2 Y
not placed
V6 T1
SM4007 SMD V7 SM4007 SMD V8
T120V/9V BV28969 GASS 1 Z1 0u1/100R
2
3
1
4
2
120V
REL120V HL1-HP-AC120
compressor XG
V1 780M05C
K1a
SM4007
V5 SM4007 SMD C20 1000u 25V
C11 100n SMD
SMD
6 V2
R28 100R SMD
1
V50
R19 47R
3
VCC
C21 10u
C12 100n SMD
C71 10u
GND
4
to drive compressor KUEHLUNG
1 D6 S21ME4
SMD R20 1k SMD
C17 2n2 Y
SM4007 SMD V62 SM4007 SMD V63
T1213
330R SMD
2 GND
to drive lid lock
V61 SM4007 SMD
6 V3
1
T1213
SM4007 SMD
Z2 0u1/100R
fans XK
R22 47R
DECKELSCHLOSS
1 D7 S21ME4
4
SMD R23 1k SMD
2 2
1
VO
SMD 25V TA
R21 1
2
G N D
2
2
lid solenoid XC
VI
2 GND
2R6 PTC R46
R27
1
6 R29 100R SMD
V4 T1213
R25 47R SMD R26 1k SMD
C18 2n2 Y
Edition: 01
Wiring Diagram Main Board
16.12.02 He
4 - 15
D8 S21ME4 4
to drive fans LUEFTER
1 330R SMD
2 GND
BIOFUGE fresco
SCHEMATIC DIAGRAMS
C2 100n SMD
auto calibration
R2 22k SMD VCC
8 3 R1
D1A TLC272 SMD 1
R4 270k SMD
VCC
VDD
2 C5 1k SMD R12 120R SMD 0.1%
temperature sensor Pt100 XU1 1 2
4
12 14 15 11
1 2 R13 100R SMD 0.1%
TEMP_1 TEMP_0
1n SMD
GND
GND
1 5 2 4 6 10 9
X0 X1 X2 X3
GND
X Y
13 3
R3 22k SMD
(74HCT4052)
6 C3 100n SMD
7 5
Y0 Y1 Y2 Y3 INH A B
Temp_1 A 0 1 1
R9 390R SMD
D1B TLC272 SMD
D2 74HC4052 SMD
C4 100n SMD
VSS
GND
VEE
R5 100K SMD
Voltage to Frequency Converter
Temp_0 X,Y B 0 100R 0 120R 1 PT100
1u
7 C7 1u
D3 LM331
8
C6 SMD 35V Ta
V S COMP IN
R/C
VCC
nicht bestueckt not placed
R11 39k SMD
R10 10k SMD
C8 10u
5 GND
SMD 35V Ta 1 6
R E F
CURR IN THRES
R6 100K SMD
G N D 4
C U R 2
F OUT
C73 10u SMD 25V TA
3
GND
TEMPERATUR
temperature signal C1 220n Fo
GND R7 12k SMD
GND
R8 2k7 SMD
GND
Edition: 01
Wiring Diagram Main Board
16.12.02 He
4 - 16
BIOFUGE fresco
SCHEMATIC DIAGRAMS not placed VCC
C22 100n SMD
GND C34 1u
C70 1u
GND
GND SMD 35V TA
D15D 74HC14 SMD 8
D10 REF RESIN CT GND TL7705
1 2 3 4
22p
SMD
GND 9
J1 J 11.14112
GND GND GND
R47 100R SMD
C24
22p
SMD
> > >
/Strom (Dreh) /Unwucht
/DECKELAUF > /DECKEL < /LUEFTER < VCC D11 8 VCC 6 NC 7 NC 5 GND 9346 GND D15B 4
R42
1 2 3 4
CS SK DI DO
6
X2
10
RESET
2 3 4 5 6 7 8 9
P1.0 P1.1 P1.2 P1.3 P1.4 P1.5 P1.6 P1.7
SMD
43 42 41 40 39 38 37 36
P2.0 P2.1 P2.2 P2.3 P2.4 P2.5 P2.6 P2.7
24 25 26 27 28 29 30 31
RD WR PSEN ALE/P TXD RXD
19 18 32 33 13 11
AD0 AD1 AD2 AD3 AD4 AD5 AD6 AD7
INH ERROR
13
GND GND INH GND
2
D15A
ALE
XF 1 14 2 13 3 12 4 11 5 10 6 9 7 8 DIP14
1
74HC14 SMD VCC 1 2 3 4
PC Burndy 4pol
SMD
GND GND VDD PH1_O ALE PH2_O A0 PH3_O 8-BIT-Modus A1 PH1_U A2 PH2_U DREHZ_IN PH3_U DREHZ_OUT GND VDD VDD WR_6X TAKT55_IN WR_7X TAKT55_OUT TEMP2 TAKT11_IN MODE PWM_IN RESET PWM_OUT1 T EI BIT9 PWM_OUT0 MN INH_IN F_IN PT U TEST F_OUT TT EE FEI I Q AARRRR T T EBNN _ QK K DDA R E E HE HH O _ T T _ _ T U G MMV L R _ _ G U I _ _ 4 5 U P NP P DE S OON T N0 1 XXRT D1 0 DRP0 1 D 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 E R G G VR G N N DO N D D DR D
VDD
C28 100n SMD
C29 100n SMD
C30 100n SMD
14 13 12 11 10 9 8
3
1 7 6 4 2 1 9 0 5 A B C D E F G DP
1 7 6 4 2 1 9 0 5
E
1 7 6 4 2 1 9 0 5
3
A B C D E F G DP
3
A B C D E F G DP
LED
RT 3MM H13 LED
F
Xf DIP14 1 1 1 1 1 4 3 2 1 0 9 8 C37 10u
1 2 3
100n 1 SMD 6 A V B C C C
Y0 Y1 Y2 Y3 Y4 6 G1 Y5 4 G G2A N Y6 5 G2B D Y7 8 D14
V18 BC807
V19 BC807
R32 1k SMD
R51 1k SMD
C32
1 2 3 4 5 6 7 1 2 3 4 5 6 7
V9 BC807
V17 BC807
1 1 1 1 1 9 8 4 3 2 1 0
13 12 11 10 9 8
V10 BC807
R52 1k SMD
R53 1k SMD
R31 1k SMD
15 14 13 12 11 10 9 7 V11
V12
V13
V14
V15
V16
V20
V53
V54
S1
S2
S3
S4
S5
S6
S7
S8
S9
V55
V56
V57
74HCT138 SMD S10 R30 4k7 SMD
H5 HDSP-5551
not placed VCC
3
GN 3MM
SMD
C31 100n SMD
H6 HDSP-5551
H7 HDSP-5551
VDD
GND 60 59 58 57 56 55 54 53 52 51 50 49 48 47 46 45 44
GND
GND VDD
GND PHASE1_O PHASE2_O PHASE3_O PHASE1_U PHASE2_U PHASE3_U
C35 10u
INHIBIT
GND
C36 10u
VSS
C69 10u
C72 10u
C27 100n SMD
C26 100n SMD
C25 100n SMD
C33 100n SMD
SMD 25V TA SMD 25V TA
1 7 6 4 2 1 9 0 5
D17A
to drive lid lock
R54 /DECKEL
/RESET
1
2
A B C D E F G DP
DECKELSCHLOSS 330R SMD
74HC14 SMD
10k SMD
SMD
R56
74HC14 D17C 3k3 6
5
signal lid open DECKELAUF
temperature signal
A B C D E F G DP
VCC SMD /UEBERSPANNUNG
3
1 7 6 4 2 1 9 0 5
3
A B C D E F G DP
R102 1k SMD
V45 BC807
V44 BC807 R103 1k SMD
R103 1k SMD
D17F 13
UEBERSTROM R57 10k SMD
10 74HC14 SMD
74HC14 SMD 3
V43 BC807
D17E 11
signal over voltage
74HC14 SMD
1 7 6 4 2 1 9 0 5
VCC 9
8
ASIC80
TEMP_0 TEMP_1 TEMPERATUR
3
R55 D17D
/DECKELAUF
4
16.12.02 He
GE 3MM H9 LED
H12 1 7 6 4 2 1 9 0 5 A B C D E F G DP
D17B
Edition: 01
A B C D E F G DP
S1 = LID S2 = Quick Run S3 = STOP S4 = START S5 = - SPEED S6 = + SPEED S7 = - TEMP S8 = +TEMP S9 = - TIME S10= + TIME
XD
D12
2 1 13 12 11 10 9 8
GND
GND
TTT EEE MMM PPP 5 4 3
19 18 17 16 15 14 13 12
Q1 Q2 Q3 Q4 Q5 Q6 Q7 Q8
C OC 74HC573
11 22 3 4 5 6 7
/RD /WR
SMD 74HC14 D15F to drive fans 12 LUEFTER 74HC14
GND
D D D D D D D D G V WR T T 7 6 5 4 3 2 1 0 NDR DOE DD U T
D16 D1 D2 D3 D4 D5 D6 D7 D8
VCC
> <
VDD
VDD WR_6X
11 1
GND
A A A A A A A A GV / / D D D D D D D D N D WR 7 6 5 4 3 2 1 0 DDRD
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
WR_6X
to drive compressor
6 6 6 6 6 6 6 6 9 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1
GND VDD ALE
2 3 4 5 6 7 8 9
> > > <
80C21PLCC PROG 0978 D15E 10 KUEHLUNG
Burndy 4pol 10k
P0.0 P0.1 P0.2 P0.3 P0.4 P0.5 P0.6 P0.7
AD0 AD1 AD2 AD3 AD4 AD5 AD6 AD7
11 1 2 3 4
SMD
H8 LED
A
H4 HDSP-5551
GN 3MM
INT0 INT1 T0 T1
R41
5
74HC14 SMD
20
XE 10k
D15C
X1
VCC
3
74HC14 SMD
D13 EA/VP
Time H3 HDSP-5551
H2 HDSP-5551
8x220R SMD
GND
21
Temp
B
R50 10k SMD
35
14 15 16 17
H1 HDSP-5551 /RESET
VCC R40 4k7 SMD
GND
C23
VCC 8 7 6 5
VCC SENSE RESET RESET SMD
signal over current GND
12 74HC14 SMD
GND
Wiring Diagram Main Board 4 - 17
BIOFUGE fresco
SCHEMATIC DIAGRAMS
XA1
U+
motor overtempemperature switch XB 1
U0 R98 10k 11W
V51 ZMM15 SMD
R87
7
+15
1
3
D34 SFH6106-3 4 SMD
3
D33 SFH6106-3 SMD
2
4 1 5
D32 TL081 SMD
R96 12k SMD
C75 10u SMD 25V TA
V49 BUZ215
6 R95 68k SMD
C63 100n SMD
Brake Resistor
R107
3
2
C65 47p SMD
4 GND
C68 470p SMD
2k2
GND
V47 BC817
V48 BC817 2k2
C61 10n SMD
SMD
R100
0R18
C62 10n SMD
C66 10u
C74 10u SMD 25V TA
DECKELAUF
SMD 1
3
2
D35 SFH6106-3 4 SMD
R94
U-
lid open signal
GND U-*
2W
R89 10k SMD 1%
/UEBERSPANNUNG
excess voltage signal
DC intermediate circuit voltage control R90 1M ZK2
C60 100n SMD
R97 12k SMD
R93 1
15R SMD
UEBERSTROM
overcurrent signal
V52 P6KE440A
2M2 SMD
R91 SMD
VCC
F2 E11T11G80 118 GRAD
R92 22k SMD
BremsWiderstand R99 1M
R88 10k SMD 1%
not placed 220R
2
C76 100u 25V SMD
C64 100n SMD
R101 470R 9W
XA lid lock, switches
2 1
2 XB1
R24 470R 9W
2 1
relative excess voltage recognition
absolute excess voltage recognition
active, if the motor put back power to the intermediate circuit
active, if the brake resistor is faulty or if the mains voltage is too high
over current recognition
Alternatively for V49: HGTP 10N50C1D
Edition: 01
Wiring Diagram Main Board
16.12.02 He
4 - 18
BIOFUGE fresco
SCHEMATIC DIAGRAMS VCC C58 10u not placed GND
C73 10u GND SMD 25V TA
C46 100n SMD GND
C45 100n SMD GND
C38 100n SMD
R76 100k SMD
V27 BC807
C54 10u SMD 25V Ta
C47 V28 BC807
GND R77
10n SMD
INHIBIT 100k SMD
GND
R65
V44 3
2
D18 SFH6106-3 4 SMD
1
3
2
D19 SFH6106-3 4 SMD
1
3
2
D20 SFH6106-3 4 SMD
1
3
2
D21 SFH6106-3 4 SMD
1
3
2
D22 SFH6106-3 4 SMD
1
3
2
D23 SFH6106-3 4 SMD
1
3
2
D24 SFH6106-3 4 SMD
220R SMD V29
R64 VCC
LL4148 R81
D26A 1
PHASE1_O 47k SMD
D26B 2
3
220R SMD 4 R66
C39 220p SMD
74HC14 SMD
74HC14 SMD
V30
LL4148 V46 ZMM12 SMD R58 1M
C55 10u
SMD
100k 2W R48
V40 LL4148
C48 1n SMD
+15
R102
R67 VCC
LL4148 R82
D26D 9
PHASE1_U 33k SMD
C40 220p SMD
D26C 8
5
74HC14 SMD
220R SMD 6
SMD
R69
R68 VCC
LL4148 R83
D26E 11
D26F 10
13
220R SMD 12 R70
C41 220p SMD
V39 LL4148
V43
220R SMD V31
47k SMD
R59 1M
C51 1n SMD
74HC14 SMD
74HC14 SMD
GND V32
R80
LL4148 V45 ZMM12 SMD R60 1M
C56 10u
SMD
100k 2W R103
V38 LL4148
C49 1n SMD
D27B 3
PHASE2_U 33k SMD
C42 220p SMD
D27A 4
1
74HC14 SMD
1
3
2
D25 SFH6106-3 4 SMD
1
3
2
D28 SFH6106-3 4 SMD
1
3
2
D29 SFH6106-3 4 SMD
220R SMD 2
3 2 1
R104
V33 D27C 5
D27F 6
13
220R SMD 12 R74
C43 220p SMD
V37 LL4148
V41
R72 VCC
47k SMD
SMD
R73 220R SMD
Phase3_O
R61 1M
C52 1n SMD
74HC14 SMD
74HC14 SMD
15R SMD
V34
R79
LL4148
100k 2W
V42 ZMM12 SMD
R105 R62 1M
C57 10u
SMD
V36 LL4148
C50 1n SMD
15R SMD
1
3
2
D30 SFH6106-3 4 SMD
1
3
2
D31 SFH6106-3 4 SMD
R106
R75 VCC D27D 9
Phase3_U 33k SMD
C44 220p SMD
D27E 8
74HC14 SMD
11
220R SMD 10
V25 BUZ215
SMD 25V TA
220R SMD
GND
LL4148 R86
V24 BUZ215
74HC14 SMD
GND
LL4148 R85
Motor motor XM1
15R SMD
R71 VCC
V23 BUZ215
SMD 25V TA
220R SMD
LL4148 R84
V22 BUZ215
15R SMD
74HC14 SMD
GND
PHASE2_O
V21 BUZ215
15R SMD
SMD 25V TA
220R SMD
GND
R78 U+
1
R63 1M SMD
V35 LL4148
C53 1n SMD
V26 BUZ215
15R SMD U-*
74HC14 SMD
GND
Edition: 01
Wiring Diagram Main Board
16.12.02 He
4 - 19
BIOFUGE fresco
DIASSEMBLY OF INSTRUMENT PARTS
The index numbers stated in ( ) reappear within the spare part figures (block diagram, schematic diagram of cooling plant) and the spare part lists. 5.1
Dismantling the Housing
5.1.1 Front Panel (100) · Open the lid. Pull out the mains plug. · Pull the unit a little over the table's rim and remove both screws (105) from the bottom of the front panel. · Pull the front panel's lower rim a few centimetre to front and push it vertically out of the upper plastic frame. ATTENTION! Do not pull the front panel too much to front! Doing so could tear off the upper plastic holders! · For continuing the disassembly remove or disconnect all wires. · Refitting the front panel is performed analogously in reverse order. 5.1.2 Lid (130) · Open the lid. Pull out the mains plug. · Remove the 4 Allen head screws (135), using a 5 mm key and take off the lid. 5.1.3 Casing (120) · Remove the front panel and the lid (see 5.1.1 and 5.1.2). · Remove the 3 Phillips screws (125) in front and both lateral. · Press both legs of the torsion spring down and fix them with a 5-mm set screw (or one of the 4 Allen head screws) in the bore of the frame. · Lift the housing at the front and the rear, pull upwards past the legs of the torsion spring and flip over to the front. If the leg is fixed with an Allen head screw, carefully widen the opening of the housing with a screw driver! · Pull off mains switch wires and take off the casing inclusive lid sealing. · Reassemble the device analogously in reverse order. · Insert the rubber sealing with the gluing seam in front and press on evenly all around
Edition:01 27.11.02 He
5-1
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.2
Replacement of the Sealing Elements
5.2.1 Motor Rubber Cover (221) Temperature probe and motor are glued to each other! When disassembling the two, damage to either or both of them cannot be excluded! · Open lid, unplug mains plug Loosen rotor nut (90) with 10-mm socket wrench (91) and pull out rotor. · Press out motor cover from the lower edge to the middle and pull upwards off the plastic flange of the motor. · Carefully pull motor cover and the two insulating washers (224) situated underneath upwards along the drive axis and deposit on the side of the temperature probe within the rotor chamber. · When the motor cover is replaced, the temperature probe must be disassembled and adjusted anew upon reassemble (see section on removal of the temperature probe)! · When the motor cover is reinserted, the upper rubber sleeve must be pressed in onto the plastic flange (231) of the motor and must be pressed down so that the ring-shaped swelling fits precisely into the groove of the flange. · Check: When finally flanging the inner sleeve lip (covers the upper rim of the plastic flange), the outer edge of the sleeve must slant downward so that condensation water may not enter the motor bearing. · For fixing and as a protection against torsion, the motor cover plus flange must be secured at the edge with 3 drops of Loctite 495. 5.2.2 Lid Gasket (123) · Unscrew the casing (120) and pull it off from the bottom plate (360). · Separate the sealing's edge from the chamber's rim by pulling it inside. · Lift off the gasket on this place. · Work all the way round, until the gasket can be removed completely. Assembling instructions · Push the gasket over the rim of the rotor chamber, starting with the gluing seam in front. · Work from both sides to the rear, but do not lengthen the gasket by this way. · While doing so press the sealing edge under the casing's rim. · Fold the rim of the rubber sealing over the rotor chamber's opening, starting from front to rear · Press the casing back again onto the bottom plate and assemble it analogously in reverse order. · The lid gasket must be completely sit close on the rotor chamber wall! · The rotor chamber must be sealed completely, when the lid is closed correctly!
Edition:01 27.11.02 He
5-2
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.3
Replacement of Mechanical Components
5.3.1 Lid Lock Assembly (210) The lid lock assembly encompasses both lid locks with two built-in microswitches each. · Disassemble housing (see 5.1.3). · Expose cables for microswitches and lid coils (undo cable tree, unscrew cable clips). · Remove outer (214) and both inner (215) fastening screws from each of the locking mechanisms. · Press microswitches (211) through the frame opening inside and pull both unlocking systems upwards and out. Note for mounting · Align rubber spacer (212) and screw locking systems on. · Do not overtighten the screws! · The locking pins must not jam! Secure Screws with Loctite 221. · Fix the cable again to the cable tree and fasten with clips. Cables may not come in between fan blades! · Once the instrument is installed, check locking mechanism several times by repeated opening and closing. 5.3.2 Torsion spring (202 · Disassemble housing (see 5.1.3) · Remove set screw(s) or Allen head screw (135) from legs in order to disassemble the housing. · Screw out fastening screw (pan-head 206) of the torsion spring and unbend the spring. · Remove fastening nut (205) of the swivel leg (203) on the side of the spring and push the shaft (200) to the left until you can remove the spring. · For removal of the shaft, press right-hand slide bush (204) out and push the shaft to the right until it leaves the left-hand slide bush. · Lift the shaft on the left and pull obliquely to the left out of the right-hand frame opening. For mounting and adjustment of the torsion spring you must follow the instructions in section 2.4, please note also the drawing on page 6-4.
Edition:01 27.11.02 He
5-4
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.4
Replacement of Electrical Components
5.4.1 Fuses (113) on main board · Dismantle the front panel (see 5.1.1) and put the device far enough to the rear so that the panel can be placed without stretching strain of wires on a suitable depot (e.g. box) in front of unit. · Check the micro fuses and if necessary replace them by equivalent and undamaged ones. · Perform a test run and search for blowing cause. · Reassemble the device analogously in reverse order. 5.4.2 NV-RAM (113) on main board · Notice old displayed NV-RAM's version number when powering on (second No. in sequence) · Depose the front panel as described in 5.4.1. · ATTENTION - CMOS components! Discharge your body before handling! · Notice correct position of NV-RAM (8 pins) and pull carefully out of socket. · Reinsert the new NV-RAM correctly. · Reassemble the device analogously in reverse order and check the displayed NV-RAM's version number. · Note! A new NV-RAM version will include parameter changes (e.g. new rotor type or any speed limitations etc.) which can be verified in the display.
5.4.3
Microcontroller (111) on main board
The microcontroller normally comes with the board. It could be possible that there will be a change sometime necessary when the will be upgrades. · Notice the old Controller's version number when powering on (first displayed No.). · Depose the front panel as described in 5.4.1. · ATTENTION - CMOS components! Discharge your body before handling! Notice the correct Controller position (CPU) and pull out carefully. ATTENTION - do not damage the socket (use cranked pliers)! · Reinsert the new Controller chip correctly. · Reassemble the device analogously in reverse order and check the displayed Controller's version number. Note! A new Controller version will include program changes (e.g. modifications of control functions) which might be verified only by measuring performances.
Edition:01 27.11.02 He
5-4
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.4.4 Main Board (110) The key and indication board is part of the main board and cannot be ordered separately! · Depose the front panel as described in 5.4.1. · Disconnect all terminal connections as: mains supply XN, compressor box XG, both lid solenoids XC and both fans XK. · Pull out all plug connectors as: protecting earth conductor PE, lid switches XA, temperature sensor XU, motor lines XM (1-3) and over-temperature cut-out XB (1+2). · Remove all fastening screws of the main (117) and indication board (115), remove the washers (116) and subsequently the boards. · Touch a grounded receptacle to discharge your body before touching the sensitive CMOS components. · Take the NV-RAM (112) out of box (or re-use the old but trouble-free component) and insert it into the socket of the new main board (see 5.4.2 and 5.4.3). · The components which is installed at the socket must be removed and scrapped · Mount the new board onto the front panel, replace the washers and re-tighten all nuts. · Do not mix up disconnected cables during re-connection! · Reassemble the device in reverse order and perform a test run, making sure the drive turns in the right direction (see imprinted arrow on rim of rotor chamber)! 5.4.5 Mains switch (121) · Remove the casing (see 5.1.3) · Compress both snap hooks of the mains switch and press the switch from inside out of the casing's opening. · Pull off the cable plugs and transfer them to the new mains switch. · Spread the snap hooks a little, refit the mains switch and assemble the unit completely. 5.4.6 Mains input socket (361) · Remove both fixing screws (365) and pull out the socket carefully · Pull off the cable plugs and transfer them to the new socket. · Insert the socket and re-tighten it on the tab of the bottom plate.
Edition:01 27.11.02 He
5-6
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.5
Replacement of Drive Components
5.5.1 Drive Motor (230) · Remove the casing (see 5.1.3). · Remove the rubber flange and both isolating disks (see 5.2.1). · Disconnect the leads for motor XM (1-3) and overtemperature switch XB (1+2) · Set the wires free from cable harness and open the cable retaining strap. · Unscrew the 3 Allen head screws (240) with a 5mm hexagon key having a length of 15cm and remove them together with the toothed discs (239). · Lift the motor a little, unscrew the grounding receptacle and remove the motor out of the spin chamber. Take care when stripping the wires through the isolation! · Reassemble the motor analogously in reverse order and fasten the 3 Allen head screws and lock them with Loctite 221. Perform the distance check as described in 2.5 and a test run. Check the sense of rotation (see imprinted arrow direction) and mix up 2 leads on terminal XM in case of disorder.
5.5.2 Motor supports (235) All 3 rubber supports (235) have to be replaced at the same time! We recommend a yearly change at every general inspection. By the way the 6 double-sided sandpaper discs (236) must be exchanged, too. A maintenance kit # 70904580 is available · Remove the drive motor (see 5.5.1). · Remove all 8 hexagon head screws (308) for the fastening of the traverse support and take it out of the frame's left side. · Unscrew the 3 nuts and remove the 3 rubber supports together with the 6 sandpaper discs. · Fasten the new motor supports evenly (use a torque key: 10 Nm) and secure the nuts (238) with screw locking lacquer (Loctite 221). · Reassemble the device in reverse order, thereby pay attention to the installation and adjustment instructions in section 2.5! At last perform a test run.
Edition:01 27.11.02 He
5-6
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.6
Replacement of Parts for the Refrigeration System
5.6.1 Temperature Sensor (220) For mounting the temperature sensor, please note the drawings page 6-5 and 6-6! · Disassemble housing (see 5.1.3) and unplug XU. · Uncover the connecting cables of the cable tree and loosen the cable clips · Disassemble motor cover (see 5.2.1) and remove complete with temperature probe (sensor). · Mount new temperature probe and new motor cover (221) according to former positioning, making sure that the tip of the probe is located within the labelling window on the motor cover (check from above, see page 6-5 figure B) · Reassemble motor cover with temperature probe according to section 5.2.1. · Adjust the distance between temperature probe and motor cover by pulling or pushing the cable for the temperature probe in the slanted opening of the motor cover until the distance between tip of the probe and motor cover is 2.5 + 0.5 mm (siehe Seite 6-5 Abbildung A) · Reassemble centrifuge in reverse order. Insert rotor and make sure that neither the temperature probe nor spacer nor temperature probe cable rub against the rotor! (siehe Seite 6-6 Abbildung C bis F) · If the adjustment is correct, fix temperature probe on both sides and the temperature probe cable at the motor cover using a drop of Loctite 495 (#20470056).
5.6.2 Fans (310) Both fans (ventilators) aspire air for cooling from the front through the ventilation slots and the wire mesh. The air is forced past the power transistors, the lamellae of the condenser and the motor windings and exits through the rear ventilation slots. Take care that the Armaflex cut (218) seals the condenser tightly against the casing. Otherwise the circulating air may form shortcuts via the slots or openings, thereby reducing cooling power and longevity of both drive and compressor. · Remove the casing (see 5.1.3) · Set the wires free from cable harness and open the cable retaining strap. · Remove the fastening screws (311), take out the defective fan. · Pull off the wire connectors, unscrew the protecting earth conductor and remove the defective fan. · Transfer grounding conductor to the new fan and reconnect the wires. · Pay attention to air flow direction (see imprinted arrow). Reassemble fan and unit in reverse order and take care to the Armaflex sealing.
Edition:01 27.11.02 He
5-7
Biofuge fresco
DIASSEMBLY OF INSTRUMENT PARTS
5.6.3 Components of the Cooling Cycle When working on the cooling cycle the general guidelines regarding the handling of refrigerants must be observed! The compressor oil must be recycled, too, in case of a compressor break down! Refrigerant: R134a (CFC-free) Filling quantity: 135 g (individually the compressor labelled amount is relevant) There must not be used any kind of hoses, armatures and manifold gauges which are already contaminated with CFC based refrigerants. Otherwise the compressor oil will become an aggressive condition and will destroy the compressor in a short time. Procedure for replacing defective components of the cooling cycle · Mount a pinch valve onto the compressor's service line (suction line) · Suck off the refrigerant and recycle it · If the compressor (340) is defective, let the oil run out into a bowl and recycle it, too. · Unsolder the defective component(s) and replace additionally the dryer cartridge (325). · Let a little amount of dry nitrogen gas stream through the tubes when soldering · Do the evacuation and refill the system with the exact amount of refrigerant via a new external dryer (check amount with a balance or filling cylinder) The pinch valve must not be left on the line! Close the system hermetically (solder close) and fix the service lines using cable ties with a little drop to let the oil run back after transportation. Re-assemble the unit in reverse order and test the cooling system (see Servicing Schedule).
Edition:01 27.11.02 He
5-8
Biofuge fresco
BREAK DOWN DRAWINGS 130
91 123
90 120
82
124
81
115
83
84
95
110
135
80 112
100
116
125
115 125
101
126
110 105 121
122 (50)
113
Edition: 01
Housing parts, Boards
16.12.02 He
6-1
Biofuge fresco
BREAK DOWN DRAWINGS 220 222 221 062 223
236 235
062
219
225 224 236
210
27.6
234
231
061 214
216 215
237 238 205
2.5
200 201
060 203
230
040
204
202
232
210
231 210
240 239
207
208
210
206
031 204 203
030
205 208
207
245 247
210
245 247
246 218 245 247
252
Edition: 01
Frame and Drive parts
16.12.02 He
6-2
Biofuge fresco
BREAK DOWN DRAWINGS
331
300
322 320 303 301 302 062
306 307 060
313 314
309
308 346
305
347 070
340 340 342 340
330
365 364
333 370
380
312 311
061
374
071
362 363 345
331 332 310
361
370
381
313 314
360
325
382
334
Edition: 01
Parts on Bottom plate
16.12.02 He
6-3
Biofuge fresco
BREAK DOWN DRAWINGS
8. Push on both legs (203) 1. Press in plain bearing bush (204) 4. Grease Molykote TP 42, ca. 5g (40) 10. Screw in dowel pin 6. Push through the shaft (200)
6. Push in the shaft (200)
5. Slip on torsion spring (202)
16., 20. Opening angle 15 - 30°
1. Press in plain bearing bush (204)
15°
17. Loctite 221 (61)
3. Push on dowel pin (201) Loctite 221 (61)
2. (5) 13. put lid (130) in place
Screw with base (206) Molykote TP 42 (40)
Bushing nipples 14x10x3 (208) 00
15. Replace set screw by screw M6x6 (135)
2. Fix screw M6x12 (207)
14. Tighten screw M6x6 (135)
16. Manually release the locks
9. Screw nut M8 (205) loosely on 12. Tighten nut M8 (205)
11. grip the shaft
11. turn to the front
(11) Loctite 221 (61)
18. Close the lid and replace the rear cup screws on both sides by set screws. Attention! Set screws must not jut out of the legs.
19. Screw lid off and mount housing. Reassemble lid as descripted above (13.) 20. Check opening angle
xx. see mounting and adjustment of the torsion spring, page 4-10/11 (xx) Index number of the spare part list
Edition: 01
Mounting of the Torsion Spring
16.12.02 He
6-4
Biofuge fresco
BREAK DOWN DRAWINGS
Figure B
position indicator
Figure A Loctite 495 (62) .6 ±0,5
Loctite 495 (62) left side
X=27
Cover (221) Y=2.5
±0,5
Temperature sensor (220)
Casing (120)
Screw M4x10 PA (223) Support (222)
front side
Edition: 01
Mounting of the Temperature Sensor
16.12.02 He
6-5
Biofuge fresco
BREAK DOWN DRAWINGS
figure C
figure D
correct
not correct
figure E
figure F
not correct
not correct
not correct
Edition: 01
Mounting of the Temperature Sensor
16.12.02 He
6-6
Biofuge fresco
Spare-Part-List
Kendro Laboratory Products Plant Osterode
BIOFUGE FRESCO 120V 60HZ HERAEUS
75005522 - 01
to Serial-No.
from Serial-No. Index
Partno.
00030 00031 00040 00050 00060 00061 00062 00070 00071 00080 00081 00082 00083 00084 00090 00091 00095 00100 00101 00105 00110 00112 00113 00115 00116 00120 00121 00122 00123 00124 00125 00126 00130 00135 00200 00201 00202 00203
20220152 20220434 20270096 20270054 20270076 20270051 20270056 20270079 20270095 70902003 20290607 75003329 20056212 75003327 70056208 20360072 20190247 20055502 20058000 20460133 20150295 70904167 20230211 20460137 20480124 20055501 20180581 20055506 20055520 20055538 20460088 20055539 70904163 20510268 70055532 20490377 20055529 70055533
Text CABLE CLIP HOSE,SHRINK MOLICOTE DELOMET 2301 SAFTY PAINT LOCTITE LOCTITE 495 FREON R134A SILFOS Rotor body O-ring ROTOR LID AEROSOL TIGHT SEAL FIXED ANGLE ROTOR 4 X PCR STRI NUT SPANNER 10 MM MAINSCABLE PANEL Overlay fresco Sorvall logo SCREW 3X14 Mainboard 100-120 V green display EEPROM FUSE 8 A, SLOW BLOWN SCREW 3X8 WASHER HOUSING MAINS SWITCH GRILL SEAL INSULATION SCREW M5 X 8 ISOLATION LID ALLEN SCREW SHAFT PIN SPRING HINGE Seite 1
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
BIOFUGE FRESCO 120V 60HZ HERAEUS
75005522 - 01
to Serial-No.
from Serial-No. Index
Partno.
00204 00205 00206 00207 00208 00210 00214 00215 00216 00218 00220 00221 00222 00223 00224 00225 00230 00231 00232 00234 00235 00236 00237 00238 00239 00240 00245 00246 00247 00252 00300 00301 00302 00303 00305 00306 00307 00308
20350220 20420139 20037565 20430215 20290408 20904501 20460133 20510271 20480244 20055507 20140614 20055511 70055509 20470253 20055524 20055525 20210349 20490376 20290567 20440288 20020732 20480257 20480251 20420070 20480251 20510137 20460094 20180138 20480108 20220510 70055515 20430216 20480251 20220472 70055516 20480193 20480251 20430216
Text BEARING NUT SCREW SCREW M6X10 SEAL Latch 120V SCREW 3X14 SCREW LOCK WASHER INSULATION Temperature sensor COVER SUPPORT SCREW, PLASTIC INSULATION INSULATION MOTOR BRACE O-RING SCREW M 3 X 6 ANTI VIBRATION MOUNT SANDWASHER SECURING WASHER NUT M 6 SECURING WASHER SCREW M 6 X 12 SCREW M 4 X 8 CONNECTOR FAN WASHER A 4,3 DOUBLE CONNECTOR MALE CHASSIS SCREW M 6 X 12 SECURING WASHER PLASTIC RING TRAVERSE WASHER SECURING WASHER SCREW M 6 X 12 Seite 2
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
BIOFUGE FRESCO 120V 60HZ HERAEUS
75005522 - 01
to Serial-No.
from Serial-No. Index
Partno.
00309 00310 00311 00312 00313 00314 00320 00321 00322 00325 00330 00331 00332 00333 00334 00340 00342 00345 00346 00347 00360 00361 00362 00363 00364 00365 00370 00374
20220530 20210255 20510056 20480244 20460094 20480108 70901150 20460151 20480135 20240477 20055517 20430207 20480244 20270072 20290568 20240481 20480251 20430221 20270072 20270072 70055514 20049932 20480193 20430216 20180608 20440023 20420076 20460104
Text ADHESIVE CLIP FAN SCREW M4X20 LOCK WASHER SCREW M 4 X 8 FAN WASHER A 4,3 EVAPORATOR BOLT M4X8 WASHER FILTER-DRYER CONDENSER SCREW M 4 X 10 LOCK WASHER ISOLATION, TAPE RUBBER COMPRESSOR SECURING WASHER SCREW M 6 X 30 ISOLATION, TAPE ISOLATION, TAPE METAL BASIS FOOT WASHER SCREW M 6 X 12 RECEPTACLE SCREW M3 X 8 HEXAGONAL NUT M4 SCREW M4X25
Seite 3
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
75005524- 01
Biofuge Frsco 120V 60Hz Sorvall
from Serial-No.
to Serial-No.
Index
Partno.
00030 00031 00040 00050 00060 00061 00062 00070 00071 00080 00081 00082 00083 00084 00090 00091 00095 00100 00101 00105 00110 00112 00113 00115 00116 00120 00121 00122 00123 00124 00125 00126 00130 00135 00200 00201 00202 00203
20220152 20220434 20270096 20270054 20270076 20270051 20270056 20270079 20270095 70902003 20290607 75003329 20056212 75003327 70056208 20360072 20190247 20055502 20058015 20460133 20150295 70904167 20230211 20460137 20480124 20055501 20180581 20055506 20055520 20055538 20460088 20055539 70904163 20510268 70055532 20490377 20055529 70055533
Text CABLE CLIP HOSE,SHRINK MOLICOTE DELOMET 2301 SAFTY PAINT LOCTITE LOCTITE 495 FREON R134A SILFOS ROOR BODY O-RING ROTOR LID AEROSOL TIGHT SEAL FIXED ANGLE ROTOR 4 X PCR STRIP NUT SPANNER 10 MM MAINSCABLE PANEL OVERLAY fresco Sorvall logo SCREW 3X14 Mainboard 100-120 V green display EEPROM FUSE 8 A, SLOW BLOWN SCREW 3X8 WASHER HOUSING MAINS SWITCH GRILL SEAL INSULATION SCREW M5 X 8 ISOLATION LID ALLEN SCREW SHAFT PIN SPRING HINGE Seite 1
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
75005524- 01
Biofuge Frsco 120V 60Hz Sorvall
from Serial-No.
to Serial-No.
Index
Partno.
00204 00205 00206 00207 00208 00210 00214 00215 00216 00218 00220 00221 00222 00223 00224 00225 00230 00231 00232 00234 00235 00236 00237 00238 00239 00240 00245 00246 00247 00252 00300 00301 00302 00303 00305 00306 00307 00308
20350220 20420139 20037565 20430215 20290408 20904501 20460133 20510271 20480244 20055507 20140614 20055511 70055509 20470253 20055524 20055525 20210349 20490376 20290567 20440288 20020732 20480257 20480251 20420070 20480251 20510137 20460094 20180138 20480108 20220510 70055515 20430216 20480251 20220472 70055516 20480193 20480251 20430216
Text BEARING NUT SCREW SCREW M6X10 SEAL Latch 120 V SCREW 3X14 SCREW LOCK WASHER INSULATION Temperature Sensor COVER SUPPORT SCREW, PLASTIC INSULATION INSULATION MOTOR BRACE O-RING SCREW M 3 X 6 ANTI VIBRATION MOUNT SANDWASHER SECURING WASHER NUT M 6 SECURING WASHER SCREW M 6 X 12 SCREW M 4 X 8 CONNECTOR FAN WASHER A 4,3 DOUBLE CONNECTOR MALE CHASSIS SCREW M 6 X 12 SECURING WASHER PLASTIC RING TRAVERSE WASHER SECURING WASHER SCREW M 6 X 12 Seite 2
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
75005524- 01
Biofuge Frsco 120V 60Hz Sorvall
from Serial-No.
to Serial-No.
Index
Partno.
00309 00310 00311 00312 00313 00314 00320 00321 00322 00325 00330 00331 00332 00333 00334 00340 00342 00345 00346 00347 00360 00361 00362 00363 00364 00365 00370 00374
20220530 20210255 20510056 20480244 20460094 20480108 70901150 20460151 20480135 20240477 20055517 20430207 20480244 20270072 20290568 20240481 20480251 20430221 20270072 20270072 70055514 20049932 20480193 20430216 20180608 20440023 20420076 20460104
Text ADHESIVE CLIP FAN SCREW M4X20 LOCK WASHER SCREW M 4 X 8 FAN WASHER A 4,3 EVAPORATOR BOLT M4X8 WASHER FILTER-DRYER CONDENSER SCREW M 4 X 10 LOCK WASHER ISOLATION, TAPE RUBBER COMPRESSOR SECURING WASHER SCREW M 6 X 30 ISOLATION, TAPE ISOLATION, TAPE METAL BASIS FOOT WASHER SCREW M 6 X 12 RECEPTACLE SCREW M3 X 8 HEXAGONAL NUT M4 SCREW M4X25
Seite 3
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
BIOFUGE FRESCO 100V INCL. ROTOR #3328
75005526- 01 from Serial-No.
to Serial-No.
Index
Partno.
00030 00031 00040 00050 00060 00061 00062 00070 00071 00080 00081 00082 00083 00084 00090 00091 00095 00100 00101 00105 00110 00112 00113 00115 00116 00120 00121 00122 00123 00124 00125 00126 00130 00135 00200 00201 00202 00203
20220152 20220434 20270096 20270054 20270076 20270051 20270056 20270079 20270095 70902003 20290607 75003329 20056212 75003327 70056208 20360072 20190247 20055502 20058015 20460133 20150295 70904167 20230211 20460137 20480124 20055501 20180581 20055506 20055520 20055538 20460088 20055539 70904163 20510268 70055532 20490377 20055529 70055533
Text CABLE CLIP HOSE,SHRINK MOLICOTE DELOMET 2301 SAFTY PAINT LOCTITE LOCTITE 495 FREON R134A SILFOS ROTOR BODY O-RING ROTOR LID AEROSOL TIGHT SEAL FIXED ANGLE ROTOR 4 X PCR STRIP NUT SPANNER 10 MM MAINSCABLE PANEL OVERLAY fresco Sorvall logo SCREW 3X14 Mainboard 100-120 V green display EEPROM FUSE 8 A, SLOW BLOWN SCREW 3X8 WASHER HOUSING MAINS SWITCH GRILL SEAL INSULATION SCREW M5 X 8 ISOLATION LID ALLEN SCREW SHAFT PIN SPRING HINGE Seite 1
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
BIOFUGE FRESCO 100V INCL. ROTOR #3328
75005526- 01 from Serial-No.
to Serial-No.
Index
Partno.
00204 00205 00206 00207 00208 00210 00214 00215 00216 00218 00220 00221 00222 00223 00224 00225 00230 00231 00232 00234 00235 00236 00237 00238 00239 00240 00245 00246 00247 00252 00300 00301 00302 00303 00305 00306 00307 00308
20350220 20420139 20037565 20430215 20290408 20904501 20460133 20510271 20480244 20055507 20140614 20055511 70055509 20470253 20054524 20055525 20210349 20490376 20290567 20440288 20020732 20480257 20480251 20420070 20480251 20510137 20460094 20180138 20480108 20220510 70055515 20430216 20480251 20220472 70055516 20480193 20480251 20430216
Text BEARING NUT SCREW SCREW M6X10 SEAL Latch 120 V SCREW 3X14 SCREW LOCK WASHER INSULATION Temperature sensor COVER SUPPORT SCREW, PLASTIC BOWL INSULATION MOTOR BRACE O-RING SCREW M 3 X 6 ANTI VIBRATION MOUNT SANDWASHER SECURING WASHER NUT M 6 SECURING WASHER SCREW M 6 X 12 SCREW M 4 X 8 CONNECTOR FAN WASHER A 4,3 DOUBLE CONNECTOR MALE CHASSIS SCREW M 6 X 12 SECURING WASHER PLASTIC RING TRAVERSE WASHER SECURING WASHER SCREW M 6 X 12 Seite 2
Mittwoch, 18. Dezember 2002
Spare-Part-List
Kendro Laboratory Products Plant Osterode
BIOFUGE FRESCO 100V INCL. ROTOR #3328
75005526- 01 from Serial-No.
to Serial-No.
Index
Partno.
00309 00310 00311 00312 00313 00314 00320 00321 00322 00325 00330 00331 00332 00333 00334 00340 00342 00345 00346 00347 00360 00361 00362 00363 00364 00365 00370 00374 00380 00381 00382
20220530 20210255 20510056 20480244 20460094 20480108 70901150 20460151 20480135 20240477 20055517 20430207 20480244 20270072 20290568 20240481 20480251 20430221 20270072 20270072 70055514 20049932 20480193 20430216 20180608 20440023 20420076 20460104 20170319 20480251 20430307
Text ADHESIVE CLIP FAN SCREW M4X20 LOCK WASHER SCREW M 4 X 8 FAN WASHER A 4,3 EVAPORATOR BOLT M4X8 WASHER FILTER-DRYER CONDENSER SCREW M 4 X 10 LOCK WASHER ISOLATION, TAPE RUBBER COMPRESSOR SECURING WASHER SCREW M 6 X 30 ISOLATION, TAPE ISOLATION, TAPE METAL BASIS FOOT WASHER SCREW M 6 X 12 RECEPTACLE SCREW M3 X 8 HEXAGONAL NUT M4 SCREW M4X25 TRANSFORMER SECURING WASHER SCREW M6 X 60
Seite 3
Mittwoch, 18. Dezember 2002
Kendro service Information
Heraeus Centrifuges PREVENTIVE MAINTENANCE CHECKLIST Rotor Inspection
Performance Checks
o o
o o o o o
Check for corrosion and wear Inspect rotor lid locking screw Serial numbers: 75003328.............................. 75003327..............................
Biofuge fresco
Type S/N
Acceleration time _________seconds instrument top speed _________rpm Deceleration time _________seconds Time (10 minute set) _________actual min:sec Temperature (4° set) _________actual (°C)
Imbalance Preliminary Checks o o o
Clean inst. & condenser coils Rotor chamber (defrost) Inst. environment (level & clearance)
Pre-run Checks o o o o o o o o
o Centrifuge stable and level o Centrifuge shuts off w/proper weight
Safety test o Accessible leakage current <3500µA o Insulation resistance >10 Megaohm o Earth Conductor Resistance <0.2 Ohm
Ground continuity Line voltage ________V AC Display segments on power-up Microprocessor Initialization (if required) Door interlock and latch Drive stop & Door unlatch (50 rpm) Compressor current draw Fan operation (condenser & compressor)
Mechanical o o
Motor bearings Refrigerant leaks
Revised – 11/11/02
1 of 2
Kendro service Information
Comments:
S.R. Signature ____________________ Date of Service ____________________
Revised – 11/11/02
2 of 2
Calibration Certification Procedure Biofuge fresco Revised - 11/11/2002 1. General Successfully completing the Preventive Maintenance Checklist and Calibration Certification Procedure as outlined will ensure that the instrument is properly maintained and calibrated to Kendro specifications. 2. Equipment Required · · · · · · ·
Digital Multimeter Temperature Meter w/probe Stopwatch Photo Tachometer Safety Tester Biofuge fresco Service Manual Centrifuge Operating Manual
Note: Check calibration due date on all test equipment before starting certification. 3. Documentation Required · · · · ·
Preventive Maintenance Procedure and Checklist (Service Manual) Calibration Certification Procedure and Form Certification Label Certificate of calibration for each piece of test equipment used to perform the certification Training Certificate Preventive Maintenance Check
· ·
Perform Preventive Maintenance checks as outlined in the Kendro Service Manual to ensure the instrument is in good working order without performing any calibrations. Complete the Preventive Maintenance Checklist. (calibration data will be filled in after Certification) Calibration Certification
·
SPEED
Install a rotor, set the speed control to 3000 rpm and start the centrifuge. When the speed has stabilized, measure the actual speed with an external tach (photo tach on multimeter where speed = frequency[no conversion]). Record measured speed and displayed speed in the speed section of the certification form. Set the speed control to max. rpm and after stabilization, record measured speed and displayed speed in the speed section of the certification form. Determine if the speeds recorded meet the specifications on the form. If the specifications are met, proceed to the next section. A recalibration of the instrument is not possible. fresco_7b_e
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Calibration Certification Procedure Biofuge fresco ·
TEMPERATURE
Place water into the rotor or bucket. Select a temperature of +4°C, maximum allowed speed of the rotor and time of minimum 3 hours. After that record the displayed temperature, measured temperature inside rotor or bucket after rotor has come to rest, in the temperature section of the certification form. Repeat the test at 15°C. Record the values in the temperature section of the certification form. Determine if the temperature values recorded meet the specifications on the form. If the specifications are met, proceed to the next section. A recalibration of the instrument is not possible. Initial the temperature section. To pre-cool the rotor select half maximum speed. ·
TIME
Using a stopwatch, check the timer by setting a 10 minute run. Start the centrifuge and measure from the time the start button is pushed until the timer switches to stop. Record the measured time in the time section of the form. Determine if the time value recorded meets the specifications in the form. If the specifications are met, proceed to the next section. A re-calibration of the instrument is not possible. Initial the time section. ·
SAFETY TEST
Using safety tester and connect the centrifuge to it. Measure Insulation resistance.. Select to Earth Conductor resistance and touch with probe all mechanical part of the centrifuge. Resistance must be <0.2 ohms at all points otherwise check for good grounding. Measure accessible current to procedure in the service manual. Documentation After filling in the certification information complete the following documentation: · · · · · ·
Log test equipment data on calibration certification form. Review P.M.checklist and calibration certification form to ensure that all data and required information has been recorded properly.(including your signature and date) Fill out a certification label and place it on the right side of the centrifuge where visible or at a location selected by the customer. Ask the customer to sign the calibration certification form and place a copy in the customer’s Certification Record Book for that centrifuge. Place copies of the P.M. checklist and Field Service Report in the same Record Book. Provide copies of your training certificate and test equipment calibration certificates if requested by the customer.
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Calibration Certification
Repair and Calibration Recertification
Preventive Maintenance Checklist Complete Account Name: Instrument Model: Report Number:
Biofuge Fresco Page 1
Account Address: Serial Number: Date:
Performance Certification
Set Speed
(rpm)
13000
Standard Test Tolerance
SPEED Rotor Used: Displayed Measured Speed Speed (rpm) (rpm)
+/-5% +/-5% Custom Test +/-5% +/-5%
Calibration Temp. (°C) +4 +15
Standard Test Tolerance (K)
TEMPERATURE Rotor Used: Displayed Measured Temp. Temp. (°C) (°C)
Passed (Initials)
Meets Spec. (y/n)
Measured Temp.after Cal. (°C)
Passed (Initials)
Meets Spec. (y/n)
Measured Time after Cal (m/s)
Passed (Initials)
Rotor Used:
Standard Test Tolerance (sec.)
10
Measured Speed after Cal.(rpm)
Rotor Used:
+/- 2 +/- 2 Custom Test +/- 2 +/- 2 +/- 2
Timer Set (min.)
Meets Spec. (y/n)
TIME Rotor Used: Measured Time (m/s)
+/- 30 Custom Test
Rotor Used: +/- 30 +/- 30 +/- 30
Type Stopwatch Temperature Meter Photo Tachometer Safety Tester
Insulations resistance >10 Megaohm
Model
TEST EQUIPMENT Serial Number
Date of Next Calibration
SAFETY TEST Earth Conductor Resistance Accessible Current <0.2 Ohm <3500µA
Revised 12/1/01 Certified By:_____________________________ Reviewed By:________________________________ Expiration Date ______ I:\TS\Service_Manual\Biofuge_fresco\230V\Fresco gruen\Fresco_7c.doc
