Jurnal Medika Veterinaria Veterinaria Erdiansyah Rahmi, Rahmi, dkk ISSN : 0853-1!3 5 IS"#$SI %$N I%EN&I'I($SI )EN*S S$#M"NE##$ %$N S+I)E##$ %$RI 'ESES "R$N)*&$N S*M$&ER$ .n/. aelii %I *S$& REIN&R"%*(SI "R$N)*&$N, J$N&+" Is.lati.n and Identi2ati.n .4 )enus Salm.nella and Shi/ella 4r.m Sumatran "ran/utan .n/. aelii 'ees 'ees in "ran/utan Reintr.duti.n Reintr.duti.n enter, Janth. Erdiansyah Rahmi 1 , %ina $/ustina 6 , dan 'aisal Jamin 3 1 #a.rat.rium +ist.l./i 'akultas 'akultas (ed.kteran +e7an *niersitas Syiah (uala, (uala, 9anda $eh 6 r./ram Studi endidikan %.kter +e7an 'akultas (ed.kteran +e7an *niersitas Syiah (uala, 9anda $eh 3 #a.rat.rium Mikr.i.l./i 'akultas 'akultas (ed.kteran +e7an *niersitas * niersitas Syiah (uala, 9anda $eh E-mail: erdianersan;/mail erdianersan;/mail.m .m $9S&R$( enelitian ini ertu
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sam=el 4eses .ran/utan semi liar diamil di =usat =ele=asliaran .ran/utan sumatera, a/ar $lam inus Janth. Sam=el diidenti2kasi den/an met.de arter arter Media selekti4 yan/ di/unakan yaitu salm.nella shi/ella a/ar SS$, e.sin methylen lue EM9, dan Ma.nkey a/ar a/ar (.l.ni ter=isah yan/ tumuh =ada media SS$ diamati m.r4.l./inya dan dilakukan =e7arnaan )ram serta dilan
>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>> >>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>> (ata kuni: 4eses, .ran/utan, Salm.nella, Shi/ella $9S&R$& &his study 7as d.ne t. identi2ed identi2ed ateria .4 /enus Salm.nella and Shi/ella Shi/ella 4r.m sumatran .ran/utan .n/. aelii 4ees Nine semi 7ild .ran/utan 4eal sam=les 7ere .tained in "ran/utan Reintr.duti.n Reintr.duti.n enter, a/ar $lam inus Janth., $eh 9esar &he sam=les 7ere =r.essed usin/ arter meth.de &he seletie media used 7ere Salm.nella Shi/ella $/ar SS$, E.sin Methylen 9lue EM9, and Ma.nkey $/ar $/ar &he se=arated .l.nies 7hih /ra7n in SS$ media 7ere .sered 4.r their m.r=h.l./y, and 4.ll.7ed y )ram stainin/ meth.de then .ntinued 7ith i.hemial test suh as Ind.l, Methyl Red MR, V./es V./es r.skauer V, Sul2d Ind.l M.tility SIM, Simm.ns itrate S, and su/ar test suh as /lu.se, sur.se, lat.se, and manit.l 'r.m 'r.m nine sam=les, .ne
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in4eted 7ith Salm.nella and Shi/ella ateria >>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>> >>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> >>>>>> (ey 7.rds: 4ees, .ran/utan, Salm.nella, Shi/ella EN%$+*#*$N "ran/utan meru=akan salah satu =rimata yan/ saat ini =alin/ teranam keeradaannya di dunia )intin/, 600? Se
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)an//uan saluran =enernaan meru=akan masalah yan/ =alin/ serin/ ditemukan =ada sat7a =rimata )an//uan ini iasanya ditandai den/an /e
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kasus klinis terin4eksi S ty=himurium men/akiatkan kematian atau dalam keadaan ham=ir mati S=esies Shi/ella meru=akan =at./en =entin/ yan/ memen/aruhi m.riditas m.riditas dan m.rtalitas m.rtalitas =ada kera )..d et al, 1? Shi/ell.sis menyeakan kematian yan/ tin//i tin//i =ada =rimata #ederer et al al 6005 6005 mela=.rkan, ditemukan dua kasus kematian .ran/utan .n/. =y/maeus di keun inatan/ Cina di $ustria ada tan//al 1 'eruari 600!, .ran/utan etina erumur ? tahun tahun men/alami kelelahan kelelahan lalu lalu timul diare erdarah 6!
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reintr.duksi =ele=asliaran =ele=asliaran .ran/utan, salah satunya adalah =usat reintr.duksi .ran/utan yan/ erada di ka7asan hutan a/ar $lam inus Janth., $eh 9esar 9esar Seelum memasuki =usat reintr.duksi terseut .ran/utan harus men
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dia7a ke #a.rat.rium Mikr.i.l./i 'akultas (ed.kteran (ed.kteran +e7an *niersitas Syiah (uala men//unakan ..l .G .G untuk dilakukan dilakukan =emeriksaan Identi2kasi akteri dilakukan den/an met.de arter 18 Identi2kasi dan Is.lasi Salm.nella dan Shi/ella 'eses dimasukkan kira-kira seanyak 1 /ram ke dalam Nutrient 9r.th dan diinkuasikan diinkuasikan selama 6!
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identi2kasi akteri )enus Salm.nella dan Shi/ella +$SI# %$N EM9$+$S$N +asil identi2kasi akteri &ael 1 terhada= sam=el 4eses .ran/utan menun
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)luk.sa B Sukr.sa B B #akt.sa B B Manit.l B B =.siti4D B ne/ati4 Salm.nell.sis dan shi/ell.sis iasanya ditandai den/an /e
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=enan/karan enyearan akteri Shi/ella Shi/ella
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(iskis B B .ti B B $yu &in/ &in/ BB $min B Ma7as *din BB 9ahruni B =.siti4D B ne/ati4 +asil identi2kasi akteri Salm.nella dan Shi/ella =ada semilan .ran/utan sumatera dida=atkan, dida=atkan, satu .ran/utan teridenti2kasi =.siti4 akteri Salm.nella dan satu .ran/utan lainnya teridenti2kasi =.siti4 akteri Shi/ella, namun seharusnya tidak ditemukan adanya akteri enter.=at./en terseut =ada 4eses .ran/utan yan/ erada di di =usat reintr.duksi reintr.duksi karena karena seelum
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enter.=at./en =ada saluran =enernaannya, 9ahruni dan $min, mun/kin ter=a=ar akteri =at./en terseut dari lin/kun/an karena kedua .ran/utan terseut telah dile=asliarkan dile=asliarkan di hutan, hu tan, dimana =ada ka7asan a/ar $lam inus Janth.
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0 hari di air, leih dari 600 hari di tanah, dan leih dari 68 sam=ai 30 ulan di dalam 4eses '.7ler dan dan Miller, 6003 In4eksi
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seara aik den/an mikr.a-mikr.a umum yan/ terda=at di dalam makanan "leh karena itu, =ertumuhannya san/at terhamat den/an adanya akteri-akteri lain, misalnya akteri =emusuk, akteri /enus Esherihia dan akteri asam laktat 9$# +al ini sesuai den/an hasil =enelitian Se=tiarini Se=tiarin i et al 6011 ah7a 9$# dari 4eses Jurnal Medika Veterin Veterinaria aria V.l V.l 8 N. 1, 'eruari 601! 8 .ran/utan .n/. =y/maeus mam=u men/hamat akteri enter.=at./en enter.=at./en Esherihia Esherihia .li, Salm.nella, dan Shi/ella karena memiliki aktiitas antimikr.a antimikr.a (ESIM*#$N %ari hasil =enelitian terhada= sam=el 4eses
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'.7ler, ME and RE Miller 6003 .. and Cild Cild $nimal Mediine 3 th ed Elseier Siene, *S$ )intin/, )intin/ , A 600? Studi Reintr.duk Reintr.duksi si "ran/utan Sumatera .n/. =y/maeus aelii #ess.n, 186 yan/ %ikeman/kan di Stasiun (arantina Medan dan di Stasiun Stasiun Reintr.duksi Reintr.duksi Jami Skri=si Institut ertanian 9./.r 9./.r )..d, R, 9% May, and & (a7at.mari 1? Enteri =ath./ens in m.nkeys m.nkeys J 9ateri.l 3:10!8-1055 +.elKer, (, $IM S7itt, and M Ciedmann 6011 $nimal .ntat as a s.ure .4 human n.n-ty=h.idal salm.nell.sisVeterinary salm.nell.sisVeterinary Researhhtt=:777 Researhhtt=:777eterinary eterinary researh.r/.ntent!613! researh.r/.ntent!613!
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transmissi.n .4 Salm.nella and am=yl.ater am=yl.ater J Si '..d $/riult 8:6!-681 Mei
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Saleh, dan $ $ Sutrisn. 600 Strate/i dan Renana Renana $ksi (.nserasi "ran/utan Ind.nesia Ind.nesia 600-601 600-601 %e=artemen (ehutanan, Jakarta &akasaka, &akasaka, M, $ $ (.hn., I Sakakiara, + Narita, and S +.n<. +.n<. 188 $n .utreak .4 salm.nell.sis in ne7ly im=.rted yn.m.l/us m.nkeys J=n J Med Si 9i.l !11:1-13 Cahyuni, & 1 9akteri Enter.=at./en =ada M.nyet Ek.r an
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Mutan-mutan den/an si4at-si4at i.kimia, anti/en dan =ath./en yan/ ereda serin/ timul dari strain induk Variasi dari entuk k.l.ni halus + men
S=esies shi/ella diklasi2kasi men
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1." #a$a %ahan Shigella sp yan/ kuran/ tahaan terhada= a/en 2sik dan kimia diandin/kan Salm.nella &ahan dalam Q @ 4en.l selama 5
$nti/ $nti/en en s.mati s.mati " shi/el shi/ella la adala adalah h li=. li=.=.l =.lisa isakh khari arida da (ek ekhu husus susan an ser.l./inya ter/antun/ =ada =.lisakarida &erda=at leih dari !0 ser.ti=e (lasi2kasi shi/ella didasarkan =ada si4at-si4at i.kimia dan anti/eni S=esies =ath./en utama di=erlihatkan =ada tale
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Setelah masa inkuasi yan / =endek 1-3 hari seara mendadak timul nyari =erut, deman dan tin
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b/ Serologi
"ran/ n.rmal serin/ mem=unyai a//lutinin terhada= eera=a s=esies Shi/ella $kan tete=i, seran/kaian seran/kaian =eneta=an anti.dy da=at menun
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hantu intelektual •
Beranda
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•
+okus (Coccus (Coccus)) dalah dalah akteri akteri !ang erentuk erentuk ulat seperti ola, dan mempun!ai mempun!ai eerapa eerapa
0ariasi seagai erikut: •
Mikrococcus, Mikrococcus, jika ke#il dan tunggal
•
Diplococcus, Diplococcus, jka ergandan!a dua*dua
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Eenus:
Escherichia
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