Biología V - Práctica de Enzima en el Hígado

C{kscfh Ghkaighf T|cth|hri|ah Tfhgrcf Gi'< ‖C|h{ji Kh{rcffhgi{ Xsagri‚ Jhrc|ah? Laifimêh U T|èkrakh bc Fhli|hri|ai gi' < ‖Cgzajh{' T|c{cgkah bc Khrhfh{h cg @êmhbi‚ T|iec{i|? Thlfi Migzèfcz Qiuhf Hsri|c{? • • • • • •

Khøh|rc Uh|cfh [h|hà Thifh Eamsc|ih I|raz Fazlcr` Efi|c{ Mi|baffi Hfhg Cjjhgscf Msraá||cz Ji|cgi Ijh| @c|gègbcz \ioh{ Nh|cg Kh|jagh @c|gègbcz Ri{kiqih [h|h M|sti? 542 <488 * <48<

<<)Giuacjl|c )<488

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Agr|ibskkaûg

Cgzajh{

Fh{ cgzajh{ {ig laikhrhfazhbi|c{ (c{ bcka| kijtsc{ri{ bc i|amcg laifûmaki! xsc hkcfc|hg fh{ |chkkaigc{ xsêjakh{' Cf t|ikc{i i|mhgazhbi bcf jcrhlifa{ji {ûfi c{ ti{alfc ti|xsc khbh káfsfh ba{tigc bc sg kigrcgabi cgzajèraki bcrc|jaghbi mcgárakhjcgrc' [ûfi bc c{rc jibi tscbcg fsmh| {ckscgkah{ bc |chkkaigc{ kii|baghbh{ (uêh{ jcrhlûfakh{!' Fh{ cgzajh{ rhjlaág th|rakathg cg jskì{ jckhga{ji{ bc |cmsfhkaûg xsc bc c{rc jibi tc|jarcg xsc cf jcrhlifa{ji {c hbhtrc h baec|cgrc{ {arshkaigc{' Kh{a ribh{ fh{ cgzajh{ {ig t|ircêgh{ tc|i rhjlaág `hq èkabi{ gskfcaki{ khrhfêrakhjcgrc hkraui{& kigikabi{ kiji ‖|ali{ijh{‚' (Niifjhg q \õ`j <442& t' 00!

Khrhfh{h

Fh khrhfh{h c{ sgh cgzajh xsc bc{kijtigc cf tc|ûpabi bc àb|ûmcgi (@
Cf jckhga{ji kijtfcri bc fh khrhfh{h gi {c kigikc& hsg h{ê fh |chkkaûg xsêjakh {c t|ibskc cg bi{ crhth{? @
T|itacbhbc{ bcf hmsh ipamcghbh Cf hmsh ipamcghbh (@
Bs|hgrc fh |chkkaûg {c falc|hg ls|lsoh{ bc ipêmcgi' Cg kigbakaigc{ gi|jhfc{ c{ri iks||c jsq fcgrhjcgrc' Ucfikabhb bc sgh |chkkaûg Fh ucfikabhb bc sgh |chkkaûg xsêjakh c{ fh khgrabhb bc t|ibskri xsc c{rh mcgc|h& i fh khgrabhb bc |chkraui xsc kig{sjc cg sgh sgabhb bc racjti' Ti| fi rhgri& fh ucfikabhb bc bc{kijti{akaûg bcf hmsh ipamcghbh tscbc jcba|{c {cmÿg fh khgrabhb bc ipêmcgi xsc {c bc{t|cgbc& ti| cocjtfi& cg sg jagsri' C{ri {c fim|h jabacgbi cf racjti q cf uifsjcg bc mh{ t|ibskabi srafazhgbi sg rsli m|hbshbi' Khrhfazhbi|c{ Cg sgh |chkkaûg fh cgc|mêh bc fi{ |chkraui{ q t|ibskri{ c{ {acjt|c fh ja{jh& tc|i fh cgc|mêh bc hkrauhkaûg tscbc ba{jagsa| {a sg khrhfazhbi| t|iti|kaigh sg khjagi hfrc|ghraui th|h xsc t|ikcbh fh |chkkaûg' Fi{ khrhfazhbi|c{ khrhfazhbi|c{ ba{jagsqcg ba{jagsqcg fh lh||c|h lh||c|h cgc|márakh cgc|márakh&& i cgc|mêh cgc|mêh bc hkrauhkaûg& hkrauhkaûg& xsc bclcg bclcg {stc|h| fi{ |chkraui{ th|h r|hg{ei|jh|{c cg t|ibskri{' Kshgbi fh cgc|mêh bc hkrauhkaûg c{ m|hgbc fh |chkkaûg iks||c fcgrhjcgrc' Sg khrhfazhbi| hsjcgrh fh ucfikabhb ti|xsc `hkc xsc fh |chkkaûg iks||h jcbahgrc sg jckhga{ji xsc |cxsac|c sgh cgc|mêh bc hkrauhkaûg jcgi|' Fi{ khrhfazhbi|c{ tscbcg {c| agi|mègaki{ s i|mègaki{' Fi{ khrhfazhbi|c{ agi|mègaki{ {scfcg {c| {c| jcrh jcrhfc fc{ { bc r|hg r|hg{a {aka kaûg ûg&& kiji kiji cf tfhr tfhragi agi q cf gêxs gêxscf cf&& i kijt kijtsc sc{r {ri{ i{ bc jcrh jcrhfc fc{ { bc r|hg{akaûg& kiji cf baûpabi bc jhgmhgc{i' C{rh{ {s{rhgkah{ tscbcg kijlagh|{c kig fi{ |chkraui{ q jibaeakh| fh bcg{abhb cfckr|ûgakh cg fh sgaûg xsêjakh xsc {c |ijtc bs|hgrc fh

|chkkaûg' Bc c{rh jhgc|h {c |cbskc fh cgc|mêh bc hkrauhkaûg q {c ehkafarh fh |chkkaûg' Cg cf kh{i bc fh bc{kijti{akaûg bcf hmsh ipamcghbh& cf khrhfazhbi| bclafarh fh sgaûg cgr|c fi{ bi{ èriji{ bc ipêmcgi (@*I*I*@!& xsc {c {cth|hg bs|hgrc cf t|ikc{i' (Hgûgaji& C{k|ari|ai bcf Bikcgrc!

Fh t|sclh bc khrhfh{h

Cg c{rh t|sclh {c ceckrÿh kig bi{ jifáksfh{ bc tc|ûpabi bc àb|ûmcgi& sgh bc fh{ kshfc{ hkrÿh kiji {s{r|hri |cbskabi q fh ir|h kiji bighbi| bc èriji{ bc àb|ûmcgi> c{ri |c{sfrh cg fh ei|jhkaûg bc hmsh ({s{r|hri |cbskabi! q ipêmcgi (bighbi| ipabhbi!'

Fh t|sclh bc khrhfh{h {c `hkc mcgc|hfjcgrc cg fèjagh (hsgxsc rhjlaág tscbc `hkc|{c cg rsli! q {c s{h kig e|ckscgkah e|ckscgkah th|h baec|cgkah| h [r|ctrikikks{ [r|ctrikikks{ (*! bc [rht`qfikikks{ (.! q @c|gègbcz& q Mh|kêh <443& t' <8=! Jak|ikikks{ (.!' (\ib|êmscz& Mhjlih& @c|gègbcz&

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Ilocraui{

8'* Bc{k|ala| fh{ kh|hkrc|ê{rakh{ bc fh khrhfh{h' <'* Ba{cøh| sg ba{ti{araui xsc tc|jarh bcrc|jagh| fh hkrauabhb cgzajèrakh bc fh{ khrhfh{h{& {s Nj q cf ceckri bc agàlabi|c{ {il|c fh hkrauabhb cgzajèrakh'

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Jcribifimêh

Eams|h 8

8! E|h{ki th|h jsc{r|h bc i|agh
Gsc{r|i ba{ti{araui kig{rh (kiji jsc{r|h cg fh eams|h 8! bc sg e|h{ki th|h jsc{r|h bc i|agh (8!& cf kshf kigracgc sgh th|rc bc `êmhbi (
(5!& xsc t|cuahjcgrc uh h kigrcgc| hmsh& h{ê cf ipamcgi kig gsc{r|i e|h{ki bc mc|lc| (5!& ffcmh|h hf e|h{ki eèkafjcgrc' Cf e|h{ki& kiji {c jsc{r|h cg fh eams|h 8 kigracgc bi{ i|aeakai{& q kiji qh {c cptfaki& ti| sgi& cgr|h|è cf ipêmcgi falc|hbi xsc uacgc ti| fh jhgmsc|h bclabi h fh |chkkaûg& jacgr|h{ xsc ti| cf ir|i sg titirc bcfmhbi (=! {c kigckrh kig fh oc|agmh (3!& (3!& qh xsc c{rh gi{ hqsbh|h h jcba| cf ipêmcgi falc|hbi ti| fh |chkkaûg> cf ipêmcgi falc|hbi t|cuahjcgrc uh h ikh{aigh| xsc cf hmsh kigrcgabh cg cf e|h{ki bc mc|lc| {slh ti| cf titirc kigckrhbi h fh oc|ag oc|agmh& mh& tc|jar tc|jaracg acgbi bi h{ê fh jcbak jcbakaûg aûg bcf ipêmcg ipêmcgi i falc|h falc|hbi bi ti| fh |chkk |chkkaûg aûg bcf `êmhbi `êmhbi jhkc|hbi . hmsh ipamcghbh i bcf `êmhbi cgrc|i . hmsh ipamcghbh'

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\c{sfrhbi{

Rhlfh{ q M|èeakh{ bcf @êmhbi {ag Jhkc|h|

Rhlfh 8 @êmhbi {ag Jhkc|h|

M|èeakh 8 @êmhbi {ag Jhkc|h|

Racjti ({!

Rsli 8 uifsjcg (jf!

< 2 5 0 84 8< 82 85 80 <4

Rhlfh < @êmhbi {ag Jhkc|h|

; 5 1 8< 83 8= 81 <4 <8 <8

M|èeakh < @êmhbi {ag Jhkc|h|

Racjti ({! < 2 5 0 84 8< 82 85 80 <4

Rsli < uifsjcg (jf! 5 1 88 8; 83 85 8= 8='3 80 80'3

Rhlfh ; @êmhbi {ag Jhkc|h|

M|èeakh ; @êmhbi {ag Jhkc|h|

Racjti ({! < 2 5 0 84 8< 82 85 80 <4

Rsli ; uifsjcg (jf! ; 5 1 8< 83 8= 81 <4 <8 <8'3

Rhlfh 2 @êmhbi {ag Jhkc|h|

M|èeakh 2 @êmhbi {ag Jhkc|h|

Racjti ({!

Rsli 2 uifsjcg (jf!

< 2 5 0 84 8< 82 85 80 <4

2 0 88 82 8= 81 <8 <8'3 << <<';

Rhlfh{ q M|èeakh{ bcf @êmhbi Jhkc|hbi

Rhlfh 3 @êmhbi Jhkc|hbi

M|èeakh 3 @êmhbi Jhkc|hbi

Racjti ({!

Rsli 3 uifsjcg (jf!

< 2 5 0 84 8< 82 85 80 <4


; 3 5 = 0 0'3 1 1'3 84'; 84'0


Racjti ({!

Rsli 5 uifsjcg (jf!

< 2 5 0 84 8< 82 85 80 <4

Rhlfh = @êmhbi Jhkc|hbi

2 0 84 88'3 8<'3 82'3 85 8='3 80'3 <4

M|èeakh = @êmhbi Jhkc|hbi

Racjti ({!

Rsli = uifsjcg (jf!

< 2 5 0 84 8< 82 85 80 <4


; = 1 84'3 88'3 8;'3 83 85'3 8='3 81


Racj Rsli 0 ti uifsjcg ({! (jf! < 2 2 0 5 84 0 8< 84 82 8< 83 82 85 85 8= 80 8='3 <4 80

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Ba{ks{aûg

Fh khrhfh{h c{ sgh cgzajh xsc bc{kijtigc cf tc|ûpabi bc àb|ûmcgi (@
káfsfh{ ti| fi xsc |ètabhjcgrc c{ bc{r|sabi ti| hkkaûg bc khrhfh{h' (Nigcjhg q [rct`cg <440& t' 8;0;!

Cg c{rc hth|rhbi kiji gi{ agbakh cf hsri|& fh khrhfh{h cg cf cptc|ajcgri ffcuhbi h khli& {a|uaû {a|uaû kiji kiji lh{c lh{c th|h th|h tibc| tibc| kshgr kshgraeak aeakh| h| fh {cth| {cth|hk hkaûg aûg bcf ipêmc ipêmcgi gi falc|h falc|hbi bi kig kac|rh kac|rh khgrabhb bc hmsh ipamcghbh' Hf jijc jijcgr gri i cg xsc xsc fh khrh khrhfh fh{h {h cgr|h cgr|hlh lh cg |chk |chkka kaûg ûg t|iu t|iuik ikhl hlh h xsc xsc {c tsba tsbac| c|h h sgh sgh mcgc|hkaûg bc ipêmcgi falc|hbi kijt|ilhgbi cf esgkaighjacgri bc á{rc rati bc cgzajh{ cg gsc{r|i i|mhga{ji'

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Kigkfs{aûg

[c fim|û kig ápari fh kig{r|skkaûg bcf ba{ti{araui bc jcbakaûg bcf ipêmcgi& qh xsc kig c{rc {c tsbi jcba| fh khgrabhb bc ipêmcgi falc|hbi kig fh |chkkaûg bcf `êmhbi jhkc|hbi . hmsh ipamcghbh q bcf `êmhbi cgrc|i . hmsh ipamcghbh& hf amshf xsc kig c{rc {c tsbi bcrc|jagh| fh ucfikabhb bc |chkkaûg bc c{ri{> {c fim|û m|hkah{ h fh khrhfh{h& xsc {c cgkh| cgkh|mh mh bc kiguc| kiguc|ra| ra| cf hmsh hmsh ipamcg ipamcghbh hbh cg hmsh hmsh q ipêmcg ipêmcgi& i& bigbc bigbc cf ipêmc ipêmcgi gi c{ falc|hbi& xscbhgbi {ifhjcgrc cf hmsh' [ê {c ksjt ksjtfa fac| c|ig ig fi{ fi{ iloc ilocra raui ui{ { bc bcrc bcrc|j |jag agh| h| fh ucfi ucfika kabh bhb b bc |chk |chkka kaûg ûg q bc fh jcbakaûg bcf ipêmcgi falc|hbi ti| fh{ |chkkaigc{ jcgkaighbh{ hgrc|ai|jcgrc' Fh{ |chkkaigc{ xsc {c bhg& {ig sg kfh|i cocjtfi bc fh{ |chkkaigc{ cpc|mûgakh{ xsc `cji{ hghfazhbi cg cf ks|{i bc Laifimêh U& hf amshf xsc fh rh{h bc |chkkaûg& xsc kiji uaji{ cg kfh{c& c{ fh khgrabhb bc |chkkaûg bc sg bcrc|jaghbi tc|aibi bc racjti'

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Lalfaim|heêh

* \ib|êmscz& C'& Mhjlih J'& @c|gègbcz& E'& q Mh|kêh& O' (<443 ( <443!' !' Lhkrc|aifimêh Mcgc|hf? T|agkatai{ q T|èkrakh{ bc Fhli|hri|ai' Fhli|hri|ai' Cbari|ahf Sgauc|{abhb bc Ki{rh \akh' * Niifjhg& O' q \õ`j& N' (<442!' Laixsêjakh' Cbari|ahf Jábakh Thghjc|akhgh' C{thøh' * Nigc Nigcjh jhg& g& C' q [rct [rct`c `cg g H' (<44 (<440! 0!'' Thghjc|akhgh' C{thøh'

ahmgû{raki Bahmgû{raki

jak|ilaifûm jak|ilaifûmaki' aki' Cbari|ahf Cbari|ahf Jábakh Jábakh

*

Hgûgaj Hgûgaji' i' Sgauc| Sgauc|{ab {abhb hb Hsrûgijh Hsrûgijh bc Kàhth Kàhth{' {' Ehksfr Ehksfrhb hb bc Kacgka Kacgkah{ h{ Xsêjak Xsêjakh{' h{' (<441!

Khrhfh{h'

Giuacjl|c <<& <488' `rrt?))c{'{k|alb'kij)bik)<4=415<3)t|hkrakh*

KHRHFH[H * Hgûgaj Hgûgaji' i' C{k|ari C{k|ari|ai |ai bcf Bikcg Bikcgrc' rc' Giuacj Giuacjl|c l|c <8& <488' <488' `rrt?))c{k|ari|aibikcgrc{' `rrt?))c{k|ari|aibikcgrc{' cbsk'h|)bhri{)khrhfazhbi|c{'`rjf

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Hsricuhfshkaûg

Hsri|c{ • • • • • •

Khøh|rc Uh|cfh [h|hà Thifh Efi|c{ Mi|baffi Hfhg Cjjhgscf Eamsc|ih I|raz Fazlcr` Msraá||cz Ji|cgi Ijh| @c|gègbcz \ioh{ Nh|cg Kh|jagh @c|gègbcz Ri{kiqih [h|h

Rirhf |chfazhbi

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Biología V - Práctica de Enzima en el Hígado

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