PEMBUATAN KEJU BAKTERI LtJctococcus 1IIctis, Streptococcus themwphiUlIS dan L e uc u c o no no s o c
m e se s e l ! ro ro id id e s
TESIS Karya tIIIis sebagai salah sato sato syar syarat at memper erol oleh eh gela gela Magi Magist ster er dari IIDtak memp Inst Instit itot ot Telm Telmol olog og Band Bandun un
OIeh:
IM Prog Progra ra
Stud Stud Biot Biotek ekno no og
INST INSTIT ITUT UT TE NOLO NOLOGI GI BA DUNG DUNG 2006
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PEMBUATAN BAKTERI
ME
KEJU
Lilcl Lilcl«o «occ ccus us lactis lactis Strep Streptoc tococ occu cu dlem dlemwpl wpllil lillu lu Leuc Leucon onos osto to
mese me sent nter eroi oide de
Oleh Isen Hariati
Prog Progra ra
Stud Stud Biot Biotek ekno nolo logi gi
Inst Instit itut ut Tekn Teknol olog og Band Bandun un
Menyetujui Pembimbing
(DR. (DR. Ping Pingka ka
Adit Aditia iawa wati ti
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ABSTRAK
Lact Lactoc ococ occu cu
lact lactis is Stre Strept ptoc ococ occu cu
ther thermo moph phil illu lu
DAN Leuconostoc
mesenteroides
Oleh Ise Hariati Hariati
ct
Leuconostoc
mesenteroides).
(Gas (Gas Chro Chroma mato togr grap aphy hy Mass Mass
Spec Spectr trop opho hoto tome metr try) y)
Parameter
fenn fennen enta tasi si da uj orga organo nole lept ptik ik
(Str (Strep epto toco cocc ccus us ther ther ophi ophil/ l/us us
Lact Lactoc ococ oc us lact lactis is dan Leuc Leucono onost stoc oc me mese sent nter eroi oide de
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hept heptad adec ecan anol ol (0,9 (0,97% 7%). ).
il dan Leucon Leuconost ostoc oc mesent mesentero eroide ides. s.
is
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ABSTRACT
Lact Lactoc ococ occu cu
/act /actis is Stre Strept ptoc ococ occu cu
thermophil/us AND Leuconostoc mesenteroides.
Streptococus ther thermo moph phil illu lus, s, Lact Lactoc ococ occu cu
lact lactis is and Leucon Leuconost ostoc oc mesent mesentero eroide ides. s.
hr mato matogr grap aphy hy Ma Spec Spectr tr ph tome tometr try) y) as plI, whey meas measur ured ed duri during ng ferm fermen enta tati tion on
of
tr
th tu
101113 celllmL).
an orga organo nole le ti
te t. Para Parame mete ters rs such such
lu io
id ta
°C
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DAFTARISI
Halaman
ABSTRAK ABSTRACT
111
ED MA
IS
IV
KATAPENGANTAR................ KATAPENGANTAR.. ............................ ............................ ............................ ............................ ........................... ............. V11
DAFTARLAMPlRAN
XI
DAFT DAFT AR GAMB GAMBAR AR
Xl11
1
L
a P
ac 11.2 11.2.2 .2 Leuc Leucon onos osto to 11.2 11.2.3 .3 Stre Strept ptoc ococ occu cu
n T
ac
10
mese me sent nter eroi oide de
10
ther thermo moph phil illu lu
11
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r S 3 P
n D
11.4 11.4.6 .6 Peng Pengel elua uara ra 7
21
Whey
P
1S
111. 111.2. 2. Pemb Pembua uata ta
Kurv Kurv Pert Pertur urnb nbuh uhan an Lact Lactoc ococ occu cu
Stre Strept ptoc ococ occu cu mesentroides 4O
lact lactis is
ther thermo moph phil il/u /us, s, dan Leuconostoc 28
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n G
8 M
Lact Lactoc ococ occu cu Stre Strept ptoc ococ occu cu
lact lactis is ther thermo mophi phill llus us
Leuc Leucon onost ostoc oc me mese sent nter eroi oide de IV.4 IV.4
Peme Pemeri riks ksaa aa
Susu Susu.. .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... ..
IV.5.1 IV.5.2 Lact Lactoc ococ occu cu
39 40 42 43
44 lact lactis is
IV.5.3 Leuc Leucon onost ostoc oc me mese sent nter eroi oide de
46 48
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Stre Strept ptoc ococ occu cu
ther thermo moph phil illu lus, s, Lact Lactoc ococ ocus us lact lactis is dan
Leucon Leuconost ostoc oc mesent mesentero eroide ide pertu pertumb mbuh uhan an masi masing ng-m -mas asin in 0,04 0,04 sel/j sel/jam am 0,04 0,04 sel/j sel/jam am dan 0,22 sel/jam,
Streptococcus ther thermo moph phil illu lus: s: Leuc Leucon onos osto to me mese sent nter eroi oide de en
la
fe
en as
am
ar ma disu disuka ka pane paneli li (4,0 (4,0), ),
Lact Lactoc ococ ocus us lact lactis is il an
dadi dadi
ebes ebesar ar 4,
ju er dan
an
adar adar asam asam lakt laktat at
5,39%.
12,25%. le ti
li
in
ak
eg
mikr mikroo oorg rgan anis isme me yang yang bert bertah ahan an hidu hidu adal adalah ah 6, enge enge re an enga enga angk angk terb terbai ai
rgan rgan le ti
ntuk ntuk ej ha il opti optima ma
adal adalah ah
ai
ju
ah
1011 sell mL.
,2 (kat (kateg egor or
is kai) kai)
,2
ming minggu gu peme pemera rama ma pada pada suhu suhu
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71
rasa rasa keju keju
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DAFT DAFTAR AR PUST PUSTAK AK
28-35. 5. Microbiology. 2, 28-3 Intern rnat atio iona na Dair Dair Fede Fede atio atio Cheese. Inte
lDF) lDF)
(Stand ndar ar Nasi Nasion onal al Indo Indone nesi sia) a) Pemer Pemeriks iksaan aan Sus Segar. Segar. SN (Sta
Handbo Handbook ok of Microb Microbiol iologi ogical cal Media. Media.
R.
Safety. 1,
1-
5.
B.
Ne
e,
Inte Intern rnet et Jour Jourjn jnal al of Food Food Prenti tice ce Hall Hall Microb Microbiol iolog og Princi Principle ple and Explor Explorat ation ion Pren
Jers Jersey ey
Foo Scienc Science. e.
Journ Journal al elect electron ronic ic
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73
Milwaukee. 16 Erte Erten, n, H. mese me sent nter eroi oide des. s. Turk Turk
Leuconostoc Agriculture.
Fisi Fisiol olog og
Ferm Fermen enta tasi si
Gizi Gizi PB Bogo Bogo
PT Medi Mediay ayat atam am
Bioi Bioind ndus ustr tr Pene Pene ap Pe ka a, Ja arta arta
an Star Starte te Orga Organi nism sm Amer Americ ic
Coun Counci cil, l, Ro emon emont. t.
2006. Chee Academ emic ic Pres Pres Cheese se Tech Techno nolo logy gy Acad
la
24. Judoam Judoamidj idjoyo oyo
Cana Cana a.
Glob Global al Anal Analyt ytic ical al
Micr Microb obio iolo logy gy Revi Review ew 12, R.
enta enta i. Penerbit
Jour Journa na Clin Clinic ical al Nutr Nutrit itio ion. n.
0. Higg Higgin ins. s. 2006 2006 Cheese. Nati Nation onal al Dair Dair R.
ekno eknolo logi gi Fe
Jerm Jerman an
FEMS
Biokonversi. Departemen
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74
Micr Microb obia ia
Cheese hedd heddar ar
Pr duct ductio io
hees heese. e.
Phys Physio iolo logy gy
John John Wile Wile
Sons,
ie
Biotechnology. 7. Phys Physio iolo logy gy of th Bact Bacter eria ia Sinaue ue Asso Associ ciat ates es Inc. Inc. Publ Publis ishe hers rs Sund Sunder erla land nd olec olecul ular ar Appr Approa oach ch Sina L.
ell. ell.
lo
it Intern rnat atio iona na Medicine. Inte
Dair Dair
la io
Food Food Asso Associ ciat atio ion. n. J. Dairy
Scinence. 84,
Carc Carcin inog ogen enic ic Agen Agent. t. J. Nutrition. 12
1055 1055
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75
Makana Makana dan Pertan Pertanian. ian. Libert Liberty, y, Yogyaka Yogyakarta rta
Pros Prosed edur ur Anal Analis is
Probio ioti tik. k. Susu Susu Ferm Fermen enta tasi si da 47 Suro Surono no 1. 2004. Prob Kary Karya, a, Jaka Jakart rta. a.
Untu Untu Baha Baha
Kese Keseha hata tan. n.
Dairy
Science. Syne Synere resi si of Curd Curd voor voor Zuiv Zuivel elon onde derz rzoe oe
(NIZ (NIZO) O) .Mel .Melku kuni nie. e.
flav flavor or an over overvi view ew
Dairy Dairy Sci
9-
il Fa Glob Globul ul Stab Stabil ilit it Lipo Lipoly lysi si with with Spec Specia ia Refe Refere renc nc to Automa Automatic tic Milkin Milking. g. octo octo al he is Up al Univ Univer er it Up al Peng Pengan anta ta Kimi Kimi Sofi Sofi
N. Pene Penerb rbit it IT
Pene Penerb rbit it ITB, ITB, Band Bandun ung. g.
Band Band ng
Orga Organi nik. k. Editor.