JOSÉ MARÍA MARÍA ARGUEDAS UNIVERSIDAD NACIONAL CARRERA PROFESIONAL DE INGENIERÍA AGROINDUSTRIAL
BIOQUÍMICA BIOQUÍMIC A DE LOS ALIMENTOS Practica N°04 TEMA DETERMINACION Y CUANTIFICACIÓN DE PROTEINAS DOCENTES ING. GUTIÉRREZ MARTÍNEZ, DENIS HERNÁN DOCENTE DE PRACTICA PR ACTICA BACH.GARCIA NAUTO, NIDIA ALUMNOS
HUAMÁN LISUNDE, MICHAEL ONCEBAY AQUINO ERICK
ANDAHUAYLA ANDAHUAYLAS S 11 DE JUNIO DE !1" !1"
INTRODUCCION
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β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OBJETIVOS - A/'(*+ -' 48&7& *&'&(48(*& /++ 7--4(#+ '+ **-#+*(<# 7- /&->#+ -#
#+ 4-0+ /&5'-4+.
FUNDAMENTO TEORICO MÉTODO DE BIURET E' -+*(2& 7- 5(- 0- *&4/- 7- (7(7& 7- 0&7(& ) 0'6+& 7- *&5- 0&'*(<# +'*+'(#+ 6--, -' @/& +4(#& 7- '+0 /&->#+0 -+**(+ * '&0 (-0 7-' *&5- 7-' -+*(2& 7+#7& -' *&'& //+, '+ *+#(7+7 7-' *&'& /&7*(7& -0 /&/&*(+' +' #4-& 7- -#'+*-0 /-/>7(*&0, ) /& '& +#& + '+ *+#(7+7 7- /&->#+. L+ /-0-#*(+ 7- /&->#+0 -# #+ 4-*'+ 0- /-7- 7--4(#+ 4-7(+#- '+ -+**(<# 7-' 5(-. E' -+*(2& 7- 5(- *(-#- *0& " -# 0&'*(<# +*&0+ +'*+'(#+ @+*(+0 + '+ /-0-#*(+ 7- N+OH & KOH. L+ -+**(<# 0- 5+0+ -# '+ 6&4+*(<# 7- # *&4/-0& 7- *&'& 2(&'-+, 7-5(7& + '+ 6&4+*(<# 7- # *&4/'-;& 7- *&&7(#+*(<# -#- '&0 (-0 *= ) '&0 /+-0 7-'-*-0 #& *&4/+(7&0 7-' #(<@-#& 3- 6&4+ /+- 7- '&0 -#'+*-0 /-/>7(*&0. R-+**(<# 7- 5(-, '+ -+**(<# 7-5- 0 #&45- +' 5(-, #+ 4&'8*'+ 6&4+7+ + /+( 7- 7&0 7- -+ #*&#*&#, 3- -0 '+ 40 0-#*(''+ 3- 7+ /&0((2+ -0+ -+**(<#, *&4# + &7&0 '&0 *&4/-0&0 3- -#@+# 7&0 & 40 -#'+*-0 /-/>7(*&0 *-*(2&0 -# 00 4&'8*'+0.
VENTAJAS • /(7& 0- /-7- *&4/'-+ -# 4-#&0 7- ! 4(#&0 • -0 -' 48&7& 40 0(4/'- 7- +#'(0(0 7- /&->#+0 • #& -0 6-*-#- -#*+ 7-(2+*(-0 7- *&'& • /&*+0 050+#*(+0 #& /&-(*+0 (#-6(--# * '+ -+**(<# 7- 5(- • #& 7--*+ N 7- 6-#-0 #& /&-(*+0 & #& /-/>7(*+0 DESVENTAJAS • #& -0 4) 0-#0(5'- *&4/++7& * -' 48&7& 7- '&9) • -3(-- 7- +' 4-#&0 + " 4@ 7- /&->#+ /& /-5+ • '+0 **-#+*(-0 +'+0 7- 0+'-0 7- +4(& (#-6(--# * '+ -+**(<# • 7-5- -0+#7+(+0- -' *&'& 4-7(+#- *+#(7+7-0 7- /&->#+ *&*(7+0
MATERIALES E EQUIPOS - S+'N+C' - +0& /-*(/(+7& - B+'+#+ +#+'>(*+ - C&*(#+ -'-*(*+ - +(''+ 7- 2(7(& - M-*-& - S&/&- M+''+ 7- +05-0& - G+7(''+ - T5& 7- -#0+)& - T-4<4-&
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METODOLOGIA M8&7& 7- 5(- p !" C#ara $% &'%() +@-@- 0&5- '&0 5&0 7- *'++ 7- -2&, ! @&+0 7N+OH +' 1!. L-@& +@-@- @&+0 7- 0&'*(<# 7CSO" +' 1. A7&/+ #+ *&'&+*(<# +' 2(&'-+ +' -0+ -# *+*& (#4-7(+& * -' -+*(2& 7- 5(- ) +' 0&4--'& -# *+'-#+4(-#& + 5+& 4+>+ ) 7-0#++'(+7&
1! 4' C'++ 7- -2& N+OH +' 1! = CS&" 5(- !@&+0 @&+0
*" G%#ati+a P-/++ '+ @-'+(#+, C&'&*+ -# 7&0 5&0 7- -#0+)& ) -+'(+ '+0 -+**(-0 )+ 4-#*(+7+0. A7&/+ #+ *&'&+*(<# @(#7+ +' -0+ -# *+*& (#4-7(+& * -' -+*(2& 7- 5(- ) +' 0&4--'& -# *+'-#+4(-#& + 5+& 4+>+ ) 7-0#++'(+7&
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CALCULOS REALI
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DISCUSI@N -
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CONCLUCION -
S- /7& A/'(*+ -' 48&7& *&'&(4->+ /& -' -0/-*&6&<4-& /++ 7--4(#+ '+ **-#+*(<# 7- /&->#+ -# #+ 4-0+ /&5'-4+.
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*6 DEFINA CON SUS PROPIAS PALABRAS QUE ES UNA CURVA PATRON"
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BIBLIOGRAFÍA H+2-) L. B(&'&@>+ *-''+ ) 4&'-*'+. B-#&0 A(-0 E7(&+ M87(*+ P+#+4-(*+#+ • *+45-'', /.#. 04(, +.7. /--0, .;. !! bioquímica ilustrada: bioquímica y biología molecular en la era posgenómica . 4+00, -0/++. • 5-@, ;.4. ;&#, '.. '5-, 0. !! bioquímica. V -7. -2-8, 5+*-'+. •