Insertos Completos de Elitech

DIAGNOSTIC REAGENTS MANUAL METHODS

Version : MMAN-09 – April 2010

SEPPIM S.A.S. ZONE INDUSTRIELLE 61500 SEES - FRANCE : 02 33 81 21 00 : 02 33 28 77 51

REAGENTS

G

A

Version GAMMA GT SL ..................... ..................... . MM-GASL-1 GAMMA GT ............................................... MM-GAGT-1

Version

ACID PHOSPHATASE ................................. MM-PACI-1

GLUCOSE HK SL ..................... ................... MM-GHSL-1 GLUCOSE PAP SL ...................................... MM-GPSL-2 GLUCOSE PAP ..................... ...................... . MM-GLUP-1

ALBUMIN .................................................. MM-ALBU-1 ALP (DEA) SL .............................................. MM-PASL-1 ALP (DEA) ................................................... MM-PALC-1

I

ALT/GPT 4+1 SL .................................... MM-ALSL4/1-1 ALT/GPT ..................................................... MM-ALAT-2

IRON CHROMAZUROL .................... ......... MM-FECA-1 IRON FERROZINE ®................... ................ MM-FEFR-2

AMYLASE SL .................... ..................... .... MM-AMSL-3 AST/GOT 4+1 SL .................................... MM-ASSL4/1-1 AST/GOT ..................................................... MM-ASAT-2

L B

LACTATE .................................................... MM-LACT-2 New

BILIRUBIN TOTAL & DIRECT 4+1 .......... MM-BITD-2

LDH-L SL................... ...................... ............. MM-LLSL-1 LDH-P .......................................................... MM-LDHP-2

M C

MAGNESIUM CALMAGITE ................... MM-MAGN-1

CALCIUM ARSENAZO ............................. MM-CALA-1 CALCIUM OCPC ........................................ MM-CALO-1 Update Update

MICROPROTEIN ........................................ MM-PRTP-2

CHLORIDE ...................... ....................... .... MM-CHLO-2

P

CHOLESTEROL SL ................... .................. MM-CHSL-2 CHOLESTEROL ..................... .................... MM-CHOL-3 CHOLESTEROL HDL SL 2G .................... MM-HDLL-1 CHOLESTEROL LDL SL 2G ...................... MM-LDLL-1 HDL CHOLESTEROL ................................ MM-HDLC-2

PHOSPHORUS ............................................ MM-PHOS-1

T

CHOLINESTERASE .................................... MM-CHES-2

TOTAL PROTEIN PLUS ....................... ...... MM-PROB-1

CK MB SL ................................................... MM-CMSL-2 CK MB ........................................................ MM-CKMB-2

TRIGLYCERIDES MONO SL NEW ...... MM-TGMLN-2 TRIGLYCERIDES ........................................ MM-TRIG-2

CK NAC SL .................................................. MM-CKSL-2 CK NAC .......................................................MM-CKNA-2

U

COPPER........................................................ MM-CUIV-1 CREATININE JAFFE ...................... ............ MM-CRCO-1 CREATININE PAP SL ................................. MM-CRSL-3

UREA UV SL ............................................... MM-URSL-2 UREA UV .................... ..................... ........... MM-URUV-1

Update

URIC ACID ................................................. MM-ACUR-2 URIC ACID MONO SL .............................. MM-AUML-2 URIC ACID SL ................... ....................... .. MM-AUSL-2


REAGENTS

G

A

Version GAMMA GT SL ..................... ..................... . MM-GASL-1 GAMMA GT ............................................... MM-GAGT-1

Version

ACID PHOSPHATASE ................................. MM-PACI-1

GLUCOSE HK SL ..................... ................... MM-GHSL-1 GLUCOSE PAP SL ...................................... MM-GPSL-2 GLUCOSE PAP ..................... ...................... . MM-GLUP-1

ALBUMIN .................................................. MM-ALBU-1 ALP (DEA) SL .............................................. MM-PASL-1 ALP (DEA) ................................................... MM-PALC-1

I

ALT/GPT 4+1 SL .................................... MM-ALSL4/1-1 ALT/GPT ..................................................... MM-ALAT-2

IRON CHROMAZUROL .................... ......... MM-FECA-1 IRON FERROZINE ®................... ................ MM-FEFR-2

AMYLASE SL .................... ..................... .... MM-AMSL-3 AST/GOT 4+1 SL .................................... MM-ASSL4/1-1 AST/GOT ..................................................... MM-ASAT-2

L B

LACTATE .................................................... MM-LACT-2 New

BILIRUBIN TOTAL & DIRECT 4+1 .......... MM-BITD-2

LDH-L SL................... ...................... ............. MM-LLSL-1 LDH-P .......................................................... MM-LDHP-2

M C

MAGNESIUM CALMAGITE ................... MM-MAGN-1

CALCIUM ARSENAZO ............................. MM-CALA-1 CALCIUM OCPC ........................................ MM-CALO-1 Update Update

MICROPROTEIN ........................................ MM-PRTP-2

CHLORIDE ...................... ....................... .... MM-CHLO-2

P

CHOLESTEROL SL ................... .................. MM-CHSL-2 CHOLESTEROL ..................... .................... MM-CHOL-3 CHOLESTEROL HDL SL 2G .................... MM-HDLL-1 CHOLESTEROL LDL SL 2G ...................... MM-LDLL-1 HDL CHOLESTEROL ................................ MM-HDLC-2

PHOSPHORUS ............................................ MM-PHOS-1

T

CHOLINESTERASE .................................... MM-CHES-2

TOTAL PROTEIN PLUS ....................... ...... MM-PROB-1

CK MB SL ................................................... MM-CMSL-2 CK MB ........................................................ MM-CKMB-2

TRIGLYCERIDES MONO SL NEW ...... MM-TGMLN-2 TRIGLYCERIDES ........................................ MM-TRIG-2

CK NAC SL .................................................. MM-CKSL-2 CK NAC .......................................................MM-CKNA-2

U

COPPER........................................................ MM-CUIV-1 CREATININE JAFFE ...................... ............ MM-CRCO-1 CREATININE PAP SL ................................. MM-CRSL-3

UREA UV SL ............................................... MM-URSL-2 UREA UV .................... ..................... ........... MM-URUV-1

Update

URIC ACID ................................................. MM-ACUR-2 URIC ACID MONO SL .............................. MM-AUML-2 URIC ACID SL ................... ....................... .. MM-AUSL-2


ELITECH

ELITECH

ELITECH

ACID

Ref. : PACI- 0030

18 x 3 mL

PHOSPHATASE

MANUAL METHOD

PROCEDURE

Wavelength Temperature Cuvette

: : :

405 nm 37°C 1 cm light path

Read against reagent blank.

Working Reagent 1 Working Reagent 2 Sample

Total acid phosphatase 1 mL 100 µL

Non-prostatic acid phosphatase 1 mL 10 µL 100 µL

Mix and after a 5 minute incubation measure the change of optical density per minute (∆OD/min.) during 3 minutes.

CALCULATION

Total ACP : NP ACP : Prostatic fraction (U/L) =

Activity (U/L) = ∆OD/min. x 853 Activity (U/L) = ∆OD/min. x 860 Total ACP (U/L) - NP ACP (U/L).

LINEARITY

If ∆OD/min. exceeds 0.070 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10.

NOTE:

For more details, see the Instructions for use

(07/2005) MM-PACI-1

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ELITECH

ELITECH

ELITECH

ALBUMIN

Ref.: ALBU - 0600 ALBU - 0700

2 x 125 mL 4 x 250 mL

MANUAL METHOD

PROCEDURE


: : :


Read against reagent blank. BLANK

Reagent Distilled Water Standard Sample

1 mL 10 µL

STANDARD

SAMPLE

1 mL

1 mL

10 µL 10 µL

Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 15 minutes.

CALCULATION

OD Sample x n OD Standard n = standard concentration

NOTE:


(07/2005) MM-ALBU-1


ELITECH

ELITECH

ELITECH

ALP (DEA) SL

Réf. : PASL- 0400 PASL- 0420 PASL- 0500

2 x 62.5 mL 4 x 62.5 mL 5 x 125 mL

MANUAL METHOD

PROCEDURE

Wavelength : 405 nm (410) Temperature : 37°C, 30°C, 25°C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure

Working reagent : Sample :

1 20

mL µL

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. • Two-reagent procedure

R1 Sample

: 1 mL : 20 µL Mix, wait 1 minute and add:

R2

:

250 µL

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes.

CALCULATION

At 405 nm: Activity (U/L) = At 410 nm: Activity (U/L) =

∆OD/min. ∆OD/min.

x x

One reagent procedure 2 750 2 910

Two reagents procedure 3424 3623

LINEARITY

If ∆OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10.

NOTE:


(07/2005) MM-PASL-1


ELITECH

ELITECH

ALP (DEA)

ELITECH

Réf. : PALC- 0030 PALC- 0200

20 x 3 mL 12 x 20 mL

MANUAL METHOD

PROCEDURE


: : :

405 nm (410) 25°C, 30°C, 37°C 1 cm light path

Read against distilled water.


1 20

mL µL


CALCULATION

At 405 nm: At 410 nm:

Activity (U/L) = Activity (U/L) =

∆OD/min.

x 2 750 ∆OD/min. x 2 910

LINEARITY

If ∆OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10.

NOTE:


(07/2005) MM-PALC-1


ELITECH

ELITECH

ELITECH

ALT/GPT 4+1 SL

Ref.: ALSL – 0410 ALSL – 0430 ALSL – 0510

2 x 62.5 mL 4 x 62.5 mL 5 x 125 mL

MANUAL METHOD

PROCEDURE

Wavelength : 340 nm Temperature : 37°C Cuvette : 1 cm light path Read against distilled water. • One-reagent procedure


1 mL 100 µL


R1 Sample


R2

:

250

µL


CALCULATION

For the two procedures, at 340 nm:

Activity (U/L) = ∆OD/min.

x

1 746

LINEARITY


NOTE:


(07/2005) MM-ALSL4/1-1


ELITECH

ELITECH

ALT/GPT

ELITECH

Ref.:

ALAT - 0200 ALAT - 0400

12 x 20 mL 9 x 50 mL

MANUAL METHOD

PROCEDURE


: : :

340 nm (334 – 365) 30°C, 37°C 1 cm light path



1 100

mL µL


CALCULATION

At 340 nm: Activity (U/L) = At 334 nm: Activity (U/L) = At 365 nm: Activity (U/L) =

∆OD/min.

x ∆OD/min. x ∆OD/min. x

1 746 1 780 3235

LINEARITY


NOTE:


(03/2008) MM-ALAT-2


ELITECH

ELITECH

AMYLASE SL

ELITECH

Réf. : AMSL - 0390 AMSL - 0400

1 x 50 mL 6 x 50 mL

MANUAL METHOD

PROCEDURE


: : :

405 nm 30°C, 37°C 1 cm light path


37 °C 1 mL 15 µL

Reagent Sample

30 °C 1 mL 25 µL


CALCULATION

At 405 nm:

Activity (U/L) =

∆OD/min.

x

37 °C 4640

30 °C 2812

Take dilution factor into account for the calculation of the activity in urine.

LINEARITY

Up to 900 U/L If ∆OD/min. exceeds 0.190 at 405 nm, repeat test using sample diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10.

NOTE:


(11/2006) MM-AMSL-3


ELITECH

ELITECH

ELITECH

AST/GOT 4+1 SL

Réf. : ASSL- 0410 ASSL- 0430 ASSL- 0510

2 x 62.5 mL 4 x 62.5 mL 5 x 125 mL

MANUAL METHOD

PROCEDURE



1 mL 100 µL


R1 Sample


R2

:

250 µL


CALCULATION



x

1 746

LINEARITY


NOTE:


(07/2005) MM-ASSL4/1-1


ELITECH

ELITECH

AST/GOT

ELITECH

Ref.:

ASAT - 0200 ASAT - 0400

12 x 20 mL 9 x 50 mL

MANUAL METHOD

PROCEDURE


: : :

340 nm (334 – 365) 30°C, 37°C 1 cm light path



1 100

mL µL


CALCULATION

At 340 nm: Activity (U/L) = At 334 nm: Activity (U/L) = At 365 nm: Activity (U/L) =

∆OD/min.

x ∆OD/min. x ∆OD/min. x

1 746 1 780 3235

LINEARITY


NOTE:


(07/2008) MM-ASAT-2


ELITECH

ELITECH

ELITECH

BILIRUBIN

Ref.: BITD – 0600

TOTAL & DIRECT 4+ 1

Total 125 mL Direct 125 mL Total 2 x 125 mL Direct 2 x 125 mL

BITO - 0600 BIDI - 0600

MANUAL M ETHOD

PROCEDURE for TOTAL BI LI RUBIN Wavelength : 550 nm

Temperature : 37°C

Cuvette : 1 cm light path


CALIBRATION 800 µL 100 µL

R1 BILIRUBIN TOTAL 4+1 Calibrator Sample

TEST 800 µL 100 µL

Mix and read the optical density (OD) after 5 minutes of incubation at 37°C OD1 OD2 Then add:

R2 BILIRUBIN TOTAL & DIRECT 4+1

200 µL

200 µL

Mix and read the optical density (OD) after 5 minutes of incubation at 37°C OD3 OD4

PROCEDURE for DI RECT BIL I RUBIN Wavelength : 550 nm

Temperature : 37°C

Cuvette : 1 cm light path


CALIBRATION 800 µL 100 µL

R1 Calibrator Sample

BILIRUBIN DIRECT 4+1

TEST 800 µL 100 µL

Mix and read the optical density (OD) after 5 minutes of incubation at 37°C OD1 OD2 Then add:

R2 BILIRUBIN TOTAL & DIRECT 4+1

200 µL

200 µL

Mix and read the optical density (OD) after 5 minutes of incubation at 37°C OD3 OD4

CALCUL ATI ON F OR BOTH PROCEDURES (Total and Direct Bil ir ubin) (OD4 - OD2 ) x F

With Factor:

mg/dL: mg/L: µmol/L:

F = 14.0 F = 140 F = 240

With cali brator:

OD4 - OD2 x n OD3 - OD1

n = Calibrator concentration

: For more details, see the Instructions for use NOTE (08/2009) MM-BITD-2 SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ELITECH

ELITECH

ELITECH

CALCIUM

Ref.: CALA - 0600

2 x 125 mL

ARSENAZO

MANUAL METHOD

PROCEDURE


: : :

650 nm (600) 37°C 1 cm light path

Read against distilled water. BLANK


1 mL 10 µL

STANDARD

SAMPLE

1 mL

1 mL

10 µL 10 µL

Mix and read the optical density (OD) after a 1 minute incubation. The final colour is stable for at least 1 hour.

CALCULATION

OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in u rine.

NOTE:


(07/2005) MM-CALA-1


ELITECH

ELITECH

ELITECH

CALCIUM OCPC

Ref.: CALO - 0600

2 x 125 mL

MANUAL METHOD

PROCEDURE


: : :


Read against distilled water. BLANK

Working Reagent Distilled Water Standard Sample

1 mL 10 µL

STANDARD

SAMPLE

1 mL

1 mL

10 µL 10 µL


CALCULATION

OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in u rine.

NOTE:


(07/2005) MM-CALO-1


ELITECH

ELITECH

ELITECH

CHLORIDE

Réf. : CHLO – 0600

2 x 125 mL

MANUAL M ETHOD

PROCEDURE


: : :

480 nm 25°C, 30°C, 37°C 1 cm light path



BLANK

STANDARD

SAMPLE

1 mL 10 µL

1 mL

1 mL

10 µL 10 µL

Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. I nformation for ur ine sample : The urine sample must be diluted ½ with distilled water before analysis.

CALCULATION Serum/Plasma:

OD Sample OD Standard



n

Urine:




n2

n= Standard concentration

NOTE : For more details, see the Instructions for use

(09/2009) MM-CHLO-2


ELITECH

ELITECH

CHOLESTEROL SL

ELITECH

Réf. : CHSL- 0490 CHSL- 0500 CHSL- 0700 CHSL- 0507 CHSL- 0707

1 6 4 6 4

x x x x x

100 100 250 100 250

mL mL mL mL* mL*

MANUAL METHOD

PROCEDURE


: : :

500 nm (492-550) 37°C 1 cm light path



1 mL 10 µL

STANDARD

SAMPLE

1 mL

1 mL

10 µL 10 µL


CALCULATION


x n


NOTE:


(07/2007) MM-CHSL-2

* these kits contain a standard

SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ELITECH

ELITECH

ELITECH

CHOLESTEROL

Ref.: CHOL - 0220 CHOL - 0420

12 x 20 mL 9 x 50 mL

MANUAL METH OD

PROCEDURE


: : :

500 nm (492 – 550) 37°C 1 cm light path



1 mL 10 µL

STANDARD

SAMPLE

1 mL

1 mL

10 µL 10 µL


CALCULATION


x n


: For NOTE

more details, see the Instructions for use

(03/2010) MM-CHOL-3


ELITECH

ELITECH

ELITECH

CHOLESTEROL

Ref.: HDLL – 0380 HDLL – 0390

1 x 80 mL 3 x 80 mL

HDL SL 2G MANUAL METHOD

PROCEDURE


: : :



R1 Distilled water Standard Sample

BLANK 900 µL 9 µL

STANDARD 900 µL

SAMPLE 900 µL

9 µL 9 µL

Mix and read absorbance (A1) after 5 minutes of incubation at 37°C. Then add:

R2

300 µL

300 µL

300 µL

Mix and read absorbance (A2) after 5 minutes of incubation at 37°C

CALCULATION

( A2 − A1 × Fdil ) Sample ( A2 − A1 × Fdil )Calibrator

×

n


Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume)

NOTE:


(04/2009) MM-HDLL-1


ELITECH

ELITECH

ELITECH

CHOLESTEROL

Ref.: LDLL – 0380

1 x 80 mL

LDL SL 2G MANUAL METHOD

PROCEDURE


: : :



R1 Distilled water Standard Sample

BLANK 900 µL 9 µL

STANDARD 900 µL

SAMPLE 900 µL

9 µL 9 µL

Mix and read absorbance (A1) after 5 minutes of incubation at 37°C. Then add:

R2

300 µL

300 µL

300 µL

Mix and read absorbance (A2) after 5 minutes of incubation at 37°C

CALCULATION

( A2 − A1 × Fdil ) Sample ( A2 − A1 × Fdil )Calibrator

×

n


Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume)

NOTE:


(04/2009) MM-LDLL-1


ELITECH

ELITECH

ELITECH

HDL CHOLESTEROL

Ref.: HDLC - 0060

3 x 10 mL

MANUAL METH OD

PROCEDURE

1) Sample preparation: Add to 500 µL of sample, 50 µL of precipitating reagent. Mix, wait for 10 minutes and centrifuge at 5000 rpm for 15 minutes. The supernatant is collected for HDL determination. 2)

HDL determination:


: : :

500 nm (492-550) 37°C 1 cm light path


Working Reagent Distilled Water Standard 50 mg/dL Supernatant

BLANK

STANDARD

SAMPLE

1 mL 50 µL

1 mL

1 mL

50 µL 50 µL


CALCULATI ON


x n x 1.1

n = Standard concentration 1.1 = Sample dilution factor

NOTE : For


(09/2009) MM-HDLC-2


ELITECH

ELITECH

CHOLINESTERASE

ELITECH

Ref.:

CHES - 0053

16 x 3 mL

MANUAL METHOD

PROCEDURE


: : :

405 nm (400 –440) 25°C, 30°C, 37°C 1 cm light path


Working reagent 1 : Sample : Working reagent 2 :

3 20 100

mL µL µL

Mix and measure the change of optical density every 30 seconds (∆OD/30 s.) during 90 seconds.

CALCULATION

Activity (U/L) =

∆OD/30

s.

x

23 460

LINEARITY

If ∆OD/30 sec. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/5 with sodium chloride solution (9 g/L). Multiply result by 10.

NOTE:


(03/2008) MM-CHES-2


ELITECH

ELITECH

CK MB SL

ELITECH

Réf. : CMSL- 0410 CMSL- 0430

2 x 62.5 mL 4 x 62.5 mL

MANUAL METHOD

PROCEDURE


: : :


Read against distilled water. • One-reagent procedure


1 40

mL µL

Mix and after a 3 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. 

• Two-reagent procedure

R1 : R2 :

1 250

mL µL

Mix and wait 30 seconds

Sample :

50

µL


CALCULATION


Activity (U/L) = ∆OD/min. x 8254

LINEARITY

Inhibition : up to 2 000 U/L of CK-MM at 37° C. If ∆OD/min. exceeds 0.075 dilute the sample (one part with nine parts saline solution), repeat the test and multiply the result by 10. Linear up at least 600 U/L.

NOTE:


(11/2008) 

: Modification from the previous version


MM-CMSL-2

ELITECH

ELITECH

CK MB

ELITECH

Réf. : CKMB- 0030

20 x 3 mL

MANUAL METHOD

PROCEDURE


: : :




1 50

mL µL


CALCULATION

a) Total CK activity : Determine total CK activity in serum using the CK-NAC reagent. b) CK-B activity : CK-B (U/L) = ∆OD/min x 3 333 c) CK-MB activity : CK-MB (U/L) = CK-B (U/L) x 2 d) Percentage of CK-MB activity in sample : % CK-MB = CK-MB x 100 Total-CK

LINEARITY

Inhibition : up to 2000 U/L of CK-MM at 37° C. If the assay of the total CK exceeds 1200 U/L at 37°C, dilute the sample appropriately with physiological saline before assay of CK-MB. Multiply the result by the dilution factor to obtain the correct value of the isoenzyme.

NOTE:


(05/2006) MM-CKMB-2


ELITECH

ELITECH

CK NAC SL

ELITECH

Réf. : CKSL- 0410 CKSL- 0430

2 x 62.5 mL 4 x 62.5 mL

MANUAL METHOD

PROCEDURE


: : :


Read against distilled water. One-reagent procedure


1 40

mL µL

Mix and after a 2 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. 


R1 R2

: 1 : 250 Mix and wait 30 seconds

mL µL

Sample

µL

:

50


CALCULATION


Activity (U/L) = ∆OD/min. x 4127

LINEARITY


NOTE:


(11/2008) 



MM-CKSL-2

ELITECH

ELITECH

CK NAC

ELITECH

Réf. : CKNA- 0030

20 x 3 mL

MANUAL METHOD

PROCEDURE


: : :

340 nm (334-365) 25°C, 30°C, 37°C 1 cm light path



1 20

mL µL


CALCULATION

At 340 nm At 334 nm At 365 nm

: : :

Activity (U/L) = ∆OD/min. Activity (U/L) = ∆OD/min. Activity (U/L) = ∆OD/min.

x x x

8 095 8 252 14 571

LINEARITY


NOTE:


(03/2008) MM-CKNA-2


ELITECH

ELITECH

ELITECH

COPPER

Réf. : CUIV- 0050

5 x 10 mL

MANUAL METHOD

PROCEDURE


: : :

580 nm (570-590) 37°C 1 cm light path

Read against reagent blank. One-reagent procedure BLANK

Working Reagent 2 Distilled Water Standard Sample

1 mL 50 µL

STANDARD

SAMPLE

1 mL

1 mL

50 µL 50 µL

Mix and read the optical density (OD) after a 5 minute incubation. • Two-reagent procedure BLANK

Working Reagent 1 Distilled Water Standard Sample

1 mL 50 µL

STANDARD

SAMPLE

1 mL

1 mL

50 µL 50 µL

Mix and read the optical density (OD1).

Reagent 2

50 µL

50 µL

50 µL

Mix and read the optical density (OD2) after a 5 minute incubation. The final colour is stable for at least 1 hour.

CALCULATION

• One-reagent procedure : OD Sample OD Standard

• Two-reagent procedure :

xn

OD2 - OD1 Sample OD2 - OD1 Standard

xn

n = standard concentration

NOTE:


(07/2005) MM-CUIV-1


ELITECH

ELITECH

CREATININE JAFFE

ELITECH

Ref.:

CRCO - 0600 CRCO - 0700

2 x 125 mL 4 x 250 mL

MANUAL METHOD

PROCEDURE


: : :

492 nm (480 –520) 37°C 1 cm light path



1 100

mL µL

Mix and read the optical density (OD1) 10 seconds after the sample or standard addition. Exactly 2 minutes after the first reading, take second reading (OD2).

CALCULATION ∆OD

Sample ∆OD Standard

xn

Take dilution factor into account for calculation of concentration in urine.

NOTE:


(07/2005) MM-CRCO-1


ELITECH

ELITECH

ELITECH

CREATININE

Ref.: CRSL - 0630

2 x 133 mL

PAP SL

MANUAL METHOD

PROCEDURE


: : :



R1 Distilled Water Standard Sample

BLANK

STANDARD

SAMPLE

1.5 mL 35 µL

1.5 mL

1.5 mL

35 µL 35 µL

Mix, and wait 5 minutes at 37°C. Then, add:

R2

0.5 mL

0.5 mL

0.5 mL

Mix and read the change of optical density (∆OD) between 1 and 4 minutes..

CALCULATION ∆OD

Sample x n

∆OD Standard

n = standard concentration Take dilution factor into account for the calculation of the concentration in u rine.

NOTE:


(03/2008) MM-CRSL-3


ELITECH

ELITECH

GAMMA GT SL

ELITECH

Ref.: GASL - 0400 GASL - 0420 GASL - 0500

2 x 62.5 mL 4 x 62.5 mL 5 x 125 mL

MANUAL METHOD

PROCEDURE


: : :


Read against distilled water. • One-reagent procedure


1 100

mL µL


R1 Sample


R2 :

250 µL


CALCULATION

At 405 nm:


One reagent procedure x 1 158

Two reagents procedure 1421

LINEARITY


NOTE:


(07/2005) MM-GASL-1


ELITECH

ELITECH

GAMMA GT

ELITECH

Ref.: GAGT – 0030 GAGT – 0200

20 x 3 mL 12 x 20 mL

MANUAL METHOD

PROCEDURE


: : :




1 mL 100 µL


CALCULATION

At 405 nm: Activity (U/L) =

∆OD/min.

x

1 111

LINEARITY


NOTE:


(07/2005) MM-GAGT-1


ELITECH

ELITECH

ELITECH

GLUCOSE HK SL

Ref.: GHSL - 0600

5 x 125 mL

MANUAL METHOD

PROCEDURE


: : :

340 nm (334-365) 37°C 1 cm light path

Read against reagent blank. • One-reagent procedure BLANK


1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL

Mix and read the optical density (OD) after a 5 minute incubation. • Two-reagents procedure BLANK

R1 Distilled Water Standard Sample

1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL

Mix, wait 1 minute and add:

R2

250 µL

250 µL

250 µL

Mix and read the optical density (OD) after a 5 minute incubation.

CALCULATION


x n


NOTE:


(07/2005) MM-GHSL-1


ELITECH

ELITECH

ELITECH

GLUCOSE PAP SL

Ref.: GPSL - 0490 GPSL - 0500 GPSL - 0700 GPSL - 0507 GPSL - 0707

1 x 100 mL 6 x 100 mL 4 x 250 mL 6 x 100 mL* 4 x 250 mL*

MANUAL METHOD

PROCEDURE


: : :

500 nm (492-550) 37°C 1 cm light path



1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL

Mix and measure the optical density (OD) after a 10 minute incubation. The reading can be done during 30 minutes.

CALCULATION


x n


NOTE:


(07/2007) MM-GPSL-2



ELITECH

ELITECH

ELITECH

GLUCOSE PAP

Ref.: GLUP - 0700 GLUP - 0800

4 x 250 mL 5 x 500 mL

MANUAL METHOD

PROCEDURE


: : :

500 nm (492-550) 37°C 1 cm light path



1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL


CALCULATION


x n


NOTE:


(07/2005) MM-GLUP-1


ELITECH

ELITECH

ELITECH

IRON

Ref.: FECA - 0600

2 x 125 mL

CHROMAZUROL

MANUAL METHOD

PROCEDURE


: : :




1 mL 50 µL

STANDARD

1 mL

SAMPLE

1 mL

50 µL 50 µL


CALCULATION


x n


NOTE:


(07/2005) MM-FECA-1


ELITECH

ELITECH

ELITECH

IRON FERROZINE

Ref.: FEFR – 0600

2 x 125 mL

MANUAL METH OD

PROCEDURE

Wavelength : 560 nm (550-570) Temperature : 37°C Cuvette : 1 cm light path Read against distilled water. BLANK


1 mL 200 µL

STANDARD

SAMPLE

1 mL

1 mL

200 µL 200 µL

Mix and read the optical density (OD).

-

OD1

OD2

50 µL

50 µL

50 µL

Then add:

R2

Mix and read the optical density (OD) within one hour.

-

OD3

OD4

CALCULATION

OD4 - OD2 x n OD3 - OD1 n = standard concentration

: For NOTE


(09/2009) MM-FEFR-2


ELITECH

ELITECH

ELITECH

LACTATE

Réf. : LACT- 0100

10 x 10 mL

MANUAL METHOD

PROCEDURE


: : :

540 nm (500-550) 37°C 1 cm light path



BLANK

STANDARD

1 mL 10 µL

1 mL

SAMPLE

1 mL

10 µL 10 µL


CALCULATION


x n


NOTE:


(05/2006) MM-LACT-2


ELITECH

ELITECH

LDH-L SL

ELITECH

Ref.: LLSL - 0400 LLSL - 0420

2 x 62.5 mL 4 x 62.5 mL

MANUAL METH OD

PROCEDURE

Wavelength : 340 nm Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank.

• Two-reagent procedure BLANK

R1 Distilled Water Sample

SAMPLE

1 mL 35 µL

1 mL 35 µL


R2

250 µL

250 µL

Mix and after a 100 second incubation, measure the change of optical density per minute ( ∆OD/min.) during 3 minutes.

CALCULATION

At 340 nm:

NOTE : For

Activity (U/L) = ∆OD/min. x

5828


(10/2007) MM-LLSL-1


ELITECH

ELITECH

LDH-P

ELITECH

Ref. : LDHP - 0030

20 x 3 mL

MANUAL METHOD

PROCEDURE


: : :

340 nm (334-365) 30°C, 37°C 1 cm light path



1 20

mL µL


CALCULATION

At 340 nm: At 334 nm: At 365 nm:

Activity (U/L) = ∆OD/min. x 8095 Activity (U/L) = ∆OD/min. x 8252 Activity (U/L) = ∆OD/min. x 15000

LINEARITY

If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/5 or 1/10 with sodium chloride solution (9 g/L). Multiply result by 5 or 10.

NOTE:


(03/2008) MM-LDHP-2


ELITECH

ELITECH

ELITECH

MAGNESIUM

Ref.: MAGN - 0600

2 x 125 mL

CALMAGITE

MANUAL METHOD

PROCEDURE


: : :

520 nm (500-550) 37°C 1 cm light path



1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL


CALCULATION


x n

n= Standard concentration Take dilution factor into account for the calculation of magnesium concentration in urine.

NOTE:


(07/2005) MM-MAGN-1


ELITECH

ELITECH

ELITECH

MICROPROTEIN

Ref.: PRTP - 0600

2 x 125 mL

MANUAL METHOD

PROCEDURE


: : :



BLANK 1 mL 20 µL


STANDARD 1 mL

SAMPLE 1 mL

20 µL 20 µL

Mix and read the optical density (OD) after a 5 minute incubation.  The final colour is stable for 10 minutes.

CALCULATION


x n


NOTE:


(11/2008) 



MM-PRTP-2

ELITECH

ELITECH

ELITECH

PHOSPHORUS

Ref.: PHOS - 0600

2 x 125 mL

MANUAL METHOD

PROCEDURE


: : :




1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL


CALCULATION


x n

n= Standard concentration Take dilution factor into account for the calculation of phosphorus concentration in urine.

NOTE:


(07/2005) MM-PHOS-1


ELITECH

ELITECH

ELITECH

TOTAL PROTEIN PLUS

Réf.: PROB - 0600 PROB - 0700

2 x 125 mL 4 x 250 mL

MANUAL METH OD

PROCEDURE


: : :



STANDARD

SAMPLE

Reagent

1 mL

1 mL

1 mL

Distilled Water

10 µL

Standard

10 µL

Sample

10 µL

Mix and read the optical density (OD) after 10 minutes incubation. The final colour is stable for 20 minutes.

CALCULATI ON




n

n= Standard concentration : For NOTE


(08/2009) MM-PROB-1 SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ELITECH

ELITECH

TRIGLYCERIDES

ELITECH

Réf. : TGML-0425 TGML-0515 TGML-0700 TGML-0427 TGML-0517 TGML-0707

MONO SL NEW

6 x 50 mL 6 x 100 mL 4 x 250 mL 6 x 50 mL* 6 x 100 mL* 4 x 250 mL*

MANUAL METHOD

PROCEDURE


: : :



STANDARD

SAMPLE

1 mL 10 µL

1 mL

1 mL


10 µL 10 µL


CALCULATION


x n


NOTE:


(07/2007) MM-TGMLN-2



ELITECH

ELITECH

ELITECH

TRIGLYCERIDES

Ref.: TRIG - 0200 TRIG - 0400

12 x 20 mL 9 x 50 mL

MANUAL METHOD

PROCEDURE


: : :

546 nm (520 – 570) 37°C 1 cm light path



1 mL 10 µL

STANDARD

1 mL

SAMPLE

1 mL

10 µL 10 µL


CALCULATION


x n


NOTE:


(07/2007) MM-TRIG-2


ELITECH

ELITECH

UREA UV SL

ELITECH

Réf. : URSL- 0400 URSL- 0420 URSL- 0500 URSL- 0407 URSL- 0427 URSL- 0507

2 4 5 2 4 5

x x x x x x

62.5 62.5 125 62.5 62.5 125

mL mL mL mL* mL* mL*

MANUAL METHOD

PROCEDURE



1 10

mL µL

∆OD) between 30 seconds and 90 seconds. Mix and read the variation of optical density (


R1 Sample

: :

1 10

mL µL


R2

:

250 µL

∆OD) between 30 seconds and 90 seconds. Mix and read the variation of optical density (

CALCULATION ∆OD

Sample

xn

∆OD Standard

n = standard concentration

NOTE:


(07/2007) MM-URSL-2



ELITECH

ELITECH

ELITECH

UREA UV

Réf. : URUV-0400 URUV-0500

9 x 50 mL 6 x 100 mL

MANUAL METHOD

PROCEDURE


: : :




BLANK

STANDARD

1 mL 10 µL

1 mL

SAMPLE

1 mL

10 µL 10 µL

Mix and read the variation of optical density (∆OD) between 30 seconds and 90 seconds.

CALCULATION ∆OD

Sample ∆OD Standard

x n

n= Standard concentration Take the dilution factor into account for the calculation of urea concentration in urine.

NOTE:


(07/2005) MM-URUV-1


ELITECH

ELITECH

URIC ACID

ELITECH

Réf. : ACUR - 0200 ACUR - 0400

12 x 20 mL 9 x 50 mL

MANUAL METH OD

PROCEDURE


: : :

510 nm (492-550) 37°C 1 cm light path


STANDARD

SAMPLE

1 mL 25 µL

1 mL

1 mL


25 µL 25 µL


CALCULATI ON


x n

n= Standard concentration Take the dilution factor into account for the calculation of uric acid concentration in urine.

NOTE : For


(09/2009) MM-ACUR-2

SEPPIM S.A..S- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ELITECH

ELITECH

URIC ACID

ELITECH

Réf. : AUML - 0420 AUML - 0500 AUML - 0700 AUML - 0427 AUML - 0507 AUML - 0707

MONO SL

6 x 50 mL 6 x 100 mL 4 x 250 mL 6 x 50 mL* 6 x 100 mL* 4 x 250 mL*

MANUAL METHOD

PROCEDURE


: : :



STANDARD

SAMPLE

1 mL 25 µL

1 mL

1 mL


25 µL 25 µL


CALCULATION


x n

n= Standard concentration Take the dilution factor into account for the calculation of uric acid concentration in urine.

NOTE:


(07/2007) MM-AUML-2



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