informe electroforesis

INFORME DE LA TÉCNICA DE ELECTROFORESIS EN GEL DE ÁCIDOS NUCLEICOS PROTOCOLO ADN – ARN

MARLIN DAYANA FERREIRA YEPES 145202804

LIC. MARIA ROCIO PARRA MOLINA

UNIVERSIDAD DE LOS LLANOS LIC. PRODUCCIÓN AGROPECUARIA ELECTIVA 2 2015

La !"#$%&%$'(' ' )* +,#%-% - !a%$a#%$(% * ! /) ' )#(!(a )*a "%$$(*# !,"#$("a "%*#$%!a-a "%* !a &(*a!(-a- - 'a$a$ (%+%!")!a' '3* ') #a+a%  "a$a !,"#$("a a #$a6,' - )*a +a#$( !a#(*%'a. F) +!a-% %$ $(+$a 6 %$ * ! a% 179: $% ') (+%$#a*"(a 6(*% a (*"$+*#a$' ")a*-% * !%' a%' "(*")*#a E. L.D)$$)+  A$* ;.<. T('!()' : (+)!'a$%* !a !"#$%&%$'(' - %*a: *%+$ /) ' a'(*= a !a 'a$a"(=* - +a#$(a!' * )* "a+% !,"#$("% * $'*"(a - a!3* #(% - '%%$#> a)*/) '# #,$+(*% ' !(+(#= %$((*a!+*# a! a*?!('(' - "%!%(-'  a$#@")!a' ')+("$%'"%("a' : ' a "%*6$#(-% * '#%' 3!#(+%' a%' * )*a +#%-%!%@a a!("a-a a ')'#a*"(a' - aB% '% +%!")!a$. E* ! '()(*# !a%$a#%$(% +!a$+%' !a #,"*("a - !"#$%&%$'(' - ADN a+!(&("a-% - !('+a*(a  !a !"#$%&%$'(' - ADN" a+!(&("a-% - ARN - 6($)' - N(!%.

OBJETIVO GENERAL

•

C%*%"$ !%' $(*"((%' ?'("%' - !a #,"*("a - !"#$%&%$'('  ') a!("a"(=* a! a*?!('(' -! ADN Pa$a ! $%"'% - *'a*aa$*-(aB - !%' '#)-(a*#' - L("*"(a#)$a * $%-)""(=* a$%")a$(a.

OBJETIVOS ESPECIFICOS

Ra!(a$ )*a !"#$%&%$'(' a$a 'a$a$ !%' &$a+*#%' - ADN: * ! !a%$a#%$(% - *,#("a a*(+a! - !a U*(6$'(-a- - !%' L!a*%'

ACTIVIDADES DE TRABAJO INDEPENDIENTE

R')!6a !a' '()(*#' $)*#a'

1.) 6a$(a!' (*#$6(** * !a #,"*("a - !"#$%&%$'(' - ?"(-%' *)"!("%'  "%+% !a a&"#a*.

1. Pureza del ADN . E! "%$# - !a *(+a - $'#$(""(=* -*- - !a )$a -!

 ADN. C%*#a+(*a*#' "%+% $%#@*a': &*%!: "!%$%&%$+%: #a*%!  a!#a' "%*"*#$a"(%*' - 'a! (*(* !a *-%*)"!a'a. 2. Temperatura  p!. ". T#p$ de m$l%&ula de ADN.  S( ! ADN *% %' )*a '")*"(a $"%*%"(-a %$ 

!a *(+a - $'#$(""(=*: '#a *(+a *% %-$? "%$#a$ ! +a#$(a! *,#("%. '. Am$rt#(uad$r )*u++er, ade&uad$. U* a+%$#()a-%$ % )&&$ $%6 !

a+(*# *"'a$(% a$a /) !a *(+a #$aaB * "%*-("(%*' =#(+a'.

2. U*a (!%a' a ")?*#% /)(6a!

E* (%!%@a +%!")!a$: ' "%*%" "%+% a$ - a' Ha$6(a-% * (*!,'  a -%' *)"!=#(-%' )("a-%' * $a' %)'#a' - ADN % - ARN "%+!+*#a$(%' /) '#?* "%*"#a-%' 6@a *!a" - (-$=*%. U* (!%a' '%* 1.000 a$' - a'' - ADN % - ARN.

. Ra!(" )* -()B% - "a-a )*% - !%' $%"-(+(*#%' - !"#$%&%$'(' $a!(a-%  -'"$@a!% * )* ?$$a&% - *% +*%' - 20 $*!%*'.

S #(* 250)! - '%!)"(=* )&&$: !)% ' (*"%$%$a * !a "?+a$a - !"#$%&%$'(': !)% ' 'a"a "%* !a +("$% (#a 10)! - )&&$ "a$a  ' "%!%"a* 1)! +a' )! - "a-a +)'#$a: * #%#a! '%* 7 +)'#$a': -'),'

4. Pa$a )'#- ")?! ' !a (+%$#a*"(a /) #(* !a #,"*("a - !"#$%&%$'(' - ?"(-%' *)"!("%' P$(+$% /) #%-% 'a+%' /) '%* 6a$(a' !a' #,"*("a' a$a '#)-(a$ !a '#$)"#)$a -! ADN: $% !a !"#$%&%$'(' ' %'(!+*# '3* !% /)  (*6'#(a-% !a +?' )'a-a * ! !a%$a#%$(% -(-% a /) ' )*a #,"*("a &"#(6a: a'#a*# &?"(! - a"$  $!a#(6a+*# "%*=+("a. C%* '#a #,"*("a ' 'a$a* +a"$%+%!,")!a' HB. $%#@*a'  ?"(-%' *)"!("%' a #$a6,' - )* &!)B% - "%$$(*# !,"#$("%.

5. E* !a a"#)a!(-a- ' a* $+!aa-% #,"*("a' %$ +#%-%!%@a' +?' '*"(!!a'  &?"(!': /) +#%-%!%@a a $+!aa-% !a #,"*("a - !"#$%&%$'(' - ?"(-%' *)"!("%' EJ!(/) "%* ')' $%(a' a!a$a' * /) "%*'('#.