GUIA - Empower HPLC

Empower PDA Software Getting Started Guide 

34 Maple Street Milford, MA 01757 71500031503, Revision A

NOTICE  The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, the use of this document.

 © 2002 WATERS WATERS CORPORATION. CORPORATION. PRINTED IN THE UNITED STA STATES OF AMERICA. ALL RIGHTS RESERVED. THIS DOCUMENT OR O R PARTS PARTS THEREOF MAY MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

Millennium and Waters are registered trademarks, and Empower is a trademark of Waters Corporation. Microsoft, Windows, and Windows NT are registered trademarks of Microsoft Corporation. All other trademarks or registered trademarks are the sole property of their respective owners.

NOTICE  The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, the use of this document.

 © 2002 WATERS WATERS CORPORATION. CORPORATION. PRINTED IN THE UNITED STA STATES OF AMERICA. ALL RIGHTS RESERVED. THIS DOCUMENT OR O R PARTS PARTS THEREOF MAY MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

Millennium and Waters are registered trademarks, and Empower is a trademark of Waters Corporation. Microsoft, Windows, and Windows NT are registered trademarks of Microsoft Corporation. All other trademarks or registered trademarks are the sole property of their respective owners.

Table of Contents Pref Pr efac ace e

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Chapter 1 PDA PD A Software Software Overview Overview .............. .......................... .......................... ............................ .......................... ............ 15 1.1 What Is PD PDA A Software? Software? ......................... ........................................ ............................. ................ .. 15 1.2 Tutorial Overview Overview........... ......................... ............................ ......................... ......................... ................. ... 17 1.3 Restoring the PD PDA A Project Project .......................... ........................................ .......................... ............ 18 Chapter 2 Viewing Vie wing PDA PDA Data .............. ............................ .......................... .......................... ............................ ..................... ....... 23 2.1 Tutorial Overview Overview........... ......................... ............................ ......................... ......................... ................. ... 23 2.2 Viewing Viewing Data Data in in Revie Review............ w.......................... ............................. ............................. ................ .. 23 2.3 Displaying Displaying the 3D 3D Plot.............. Plot ............................ ............................ ............................ ................. ... 29 2.4 Zooming In on Plots ......................... .................................... ......................... .......................... ............ 31 2.5 Extracting Extracting a Chromatogra Chromatogram m ........................ ...................................... .......................... ............ 33 2.6 Extracting Extracting a Spectrum........................ Spectrum...................................... ........................... ..................... ........ 36 2.7 Annotating Annotating Chromatograms Chromatograms and Spectra............................ Spectra.............................. .. 41 2.7.1 Adding Annotations............... Annotations............................. ......................... ....................... ............ 42 2.7.2 Erasing Annotations........... Annotations......................... ............................ .......................... ............ 45 2.7.3 Annotation Annotation Tools Tools ......................... ...................................... ........................... .................. .... 46 Chapter 3 Peak Purity Process Processing ing ......... ....................... ............................ ............................ ............................ ................. ... 47 3.1 Tutorial Overview Overview........... ......................... ............................ ......................... ......................... ................. ... 47 3.2 Deriving Chromatograms Chromatograms ....................... .................................... ........................... .................. .... 48

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3.3 Developing a PDA Processing Method ................................. 51 3.3.1 Viewing, Modifying and Saving the Method Set......... 65 3.3.2 Viewing the Peak Purity Calculation .......................... 67 3.4 Reviewing Peak Purity Results ............................................. 69 3.4.1 Using Spectrum Index ............................................... 69 3.4.2 Using the Results Window......................................... 75 Chapter 4 Library Matching .............................................................................. 80 4.1 Tutorial Overview................................................................... 80 4.1.1 Steps in Creating a Library ........................................ 80 4.1.2 Steps in Library Matching .......................................... 81 4.2 Creating a New Library ......................................................... 82 4.3 Matching Spectra to a Library ............................................... 89 4.3.1 Modifying an Existing Processing Method for Library Matching .................................................................... 89 4.3.2 Creating a New Processing Method for Library Matching .................................................................... 94 4.3.3 Performing Library Matching.................................... 107 4.4 Reviewing Library Matching Results................................... 109

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Chapter 5 Printing Reports ............................................................................. 114 5.1 Previewing a Report............................................................ 114 5.2 Background Printing............................................................ 117 Appendix A Default PDA Reports ...................................................................... 120 Index

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List of Figures 1-1 1-2 1-3 1-4 1-5

Empower Login Dialog Box............................................................ 18 Empower Pro Window ................................................................... 19 Configuration Manager .................................................................. 20 Restore Project Wizard - Start Software Page .............................. 21 Configuration Manager with Restored PDA_Default Project ......... 22

2-1 2-2 2-3 2-4 2-5 2-6 2-7 2-8 2-9 2-10 2-11 2-12 2-13 2-14 2-15 2-16 2-17 2-18 2-19 2-20 2-21 2-22

Empower Pro Window ................................................................... 24 Browse Project Dialog Box ............................................................ 24 Project Window.............................................................................. 25 Review with PDA Data, no Chromatogram Extracted.................... 26 Maximized Review Main Window in 3D Layout ............................. 27 Review Toolbar Buttons ................................................................. 28 Sample 3D Plot Window ................................................................ 30 Creating the Zoom Box .................................................................. 31 Zoomed View of the Contour Plot.................................................. 32 Scaling Tab .................................................................................... 33 Extracted Chromatogram at Wavelength 254 nm .......................... 34 Extracted Chromatogram at Wavelength 280 nm .......................... 35 Overlaid Chromatograms at Wavelengths 254 and 280 nm .......... 36 Extracted Spectrum at 1.388 Minutes............................................ 37 Overlaid Extracted Spectra............................................................ 38 Spectra Table ................................................................................. 39 Normalized Spectra ....................................................................... 40 Viewing Spectrum Points: Absorbances Versus Wavelengths....... 41 Annotation Tools ............................................................................ 42 Annotation Object Properties Dialog Box for an Object ................. 43 Annotation Object Properties Dialog Box for Text .......................... 44 Annotation Example....................................................................... 45

List of Figures

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