Dye Reduction Test Abdulloh Khudry and Aprillia Fatma Ely

UNIVERSITAS PANCASILA PROGRAM MAGISTER ILMU KEFARMASIAN

TUGAS I UJI MUTU “DYE REDUCTION TEST” 

OLEH ABDULLOH KHUDRY

5416221071

APRILIA FATMA ELY

5416221076

JAKARTA 2018

I.

Latar belakang Beber! !er"#$% !e%&$#'( )e%*e& )"!"% +e," )e-+'%' b$. #"#" /e%&% re%/$ b+*er' #eb&' b$% /#r%- +% )e%&$#'(+% !r/"+  /e%&% +"('*# -%& *'%&&' A/%- )'+rr&%'#)e /() !r/"+ )+%% $#'( ($% #"#" /% /&'%& )e%,/' #($ #*" !e%e%*" +"('*# /r' !r/"+  *er#eb"* De%&% !er+e)b%&% '()" !e%&e*$"% /% *e+%(&' /'$r!+% /!* )e)b%*" )e)!erb'+' $#'( !r/"+#' )+%% $#'( ($% #"#" /% /&'%& Se$'%&& /'b"*"$+% !r#e/"r -%& /!* )e%&$'*"%& *" )e%&"+"r +%/"%&% b+*er' /() !e)b"*% )e%*e& +e," b$+% !r/"+ ('% -%& )e%&&"%+% #"#" /% /&'%& #eb&' b$% /#r%- 3H#*'%&# /++ 122 Me*/e !e%e%*"% ,")($ )'+rr&%'#)e /() #"*" !r/"+ )+%% b'#%- /'(+"+% /e%&% !er$'*"%&% ,")($ b+*er' /e%&% )e*/e  plate count  )"% )e*/e *er#eb"* )e)b"*"$+% .+*" -%& re(*' () /'+re%+% +eb"*"$% +% )e*/e -%& e!* /% )"/$ "%*"+ !e%&"+"r% +"('*# b$% /#r #e!er*' #"#" /% /&'%& !r !e%e('*' )e%e)"+% )e*/e Rapid Dye Reduction Test  Me*/e *er#eb"* )e%&&"%+% !e.r% methylen blue, resazurin  /% tetrazolium "%*"+ )e%e%*"+% +"('*# )'+rb' /() !r/"+  #"#" '+% -) /% /&'%& 3R /% M"r*$- 186

II.

Pembahasan Pe%&&"%% )e*/e Dye Reduction Test  /'+e%( /%- 9 #e%-. !e.r% -%& )e%,/' '%/'+*r /() !e%e%*"% ,")($ b+*er' I%/'+*r !er*) /($ Methylene Blue 3MB #e$'%&& )e*/e%- /'+e%( /e%&% Methylene Blue Dye Reduction Test  3MBRT Pr'%#'! /r' )e*/e '%' /($ !er"b$% .r% b'r" /r' '%/'+*r )e%,/' *'/+ ber.r% $( '%' /'+re%+% /%- !r#e# )e*b('#)e /r' )'+rr&%'#)e *er$/!

'%/'+*r *er#eb"* Kre% /%- !erbe/% !er"b$% .r% /r' '%/'+*r MB /!* /'*e%*"+% ,")($ )'+rr&%'#)e /e%&% )e*/e Colony Forming Unit   3:FU Me*/e MBRT #"/$ /'&"%+% !/ !r/"+ #"#" /% )'%")% "%*"+ )e('$* **( +e#e("r"$% )'+rr&%'#)e #e!er*' E (' /% B #"b*'('# 3%/- /% ;e%+*e#$ 2010 I%/'+*r !e.r% +e/" /'+e%( /e%&% %) Resazurin  -%& /'+e%( /e%&% )e*/e Resazurin Tube Method   3RTM Resazurin  )er"!+% '%/'+*r re/< /')% '%/'+*r *er#eb"* +% *ere/"+#' )e%,/' re#r"='% +re% /%- )e*b('#)e /r' )'+rr&%'#)e H"b"%&% /%- re/"+#' Resazurin )e%,/' re#r"='% )e%"%,"+% /%- !er*")b"$% /% !er+e)b%&% )'+rr&%'#)e Per"b$% .r% /r' Re#>"r'% -%& ber.r% b'r" )e%,/' )er$ )"/ /r' re#r"='% )er"!+% !r'%#'! /r' )e*/e '%' 3P*'( /++ 2014 Tetrazolium )er"!+% !e.r% '%/'+*r +e*'& (r"*% !e.r% tetrazolium 31? b'#%- *erb"* /r' Nitroblue tetrazolium 3BT *" 2@34@ Iodophenyl @9@34@Nitrophenyl @5@ phenyl Tetrazolium 3IT D')% (r"*% tetrazolium -%& *'/+ ber.r% +% ber"b$ )e%,/' b'r" ,'+ *erb"* /r' Nitroblue tetrazolium /% +% ber"b$ )e%,/' )er$ )"/ ,'+ *erb"* /r' 2@34@Iodophenyl @9@34@Nitrophenyl @5@ phenyl Tetrazolium 3R /%

M"r*$-

186 III

Ke#')!"(% 1 S"#" /e%&% re%/$ b+*er' #eb&' b$% /#r%- +% )e%&$#'(+% !r/"+ /e%&% +"('*# -%& *'%&&' 2 A/%- )'+rr&%'#)e /() !r/"+ )+%% $#'( ($% #"#" /% /&'%& )e%,/' #($ #*" !e%e%*" +"('*# /r' !r/"+ *er#eb"* 9 U%*"+ !e%&"+"r% +"('*# b$% /#r #e!er*' #"#" /% /&'%& /e%&% )e*/e -%& e!* /% )"/$ /!* )e%&&"%+% Rapid Dye Reduction Test 

4 Rapid D-e  Reduction Test   )e%&&"%+% 9 '%/'+*r -'*" Methylene Blue 3MB

Resazurin

/%

Tetrazolium

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/'*e%*"+%

,")($

)'+rr&%'#)e /e%&% )e*/e Colony Forming Unit   3:FU ,'+ *er,/' !er"b$% .r% -%& #e#"'

AFTAR PUSTAKA

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