Cromatografía de Intercambio iónico

Bpgnk}gfpkiîk ma de}apbkn`dg de}apbkn`dg dòedbg

Hdr}gpdk

Cgr nä}gmgr dòedbgr hke rdmg q}dcd|kmgr marma ;81:# bqkemg H% ]hgnzrge t L% ]% Vkt# deyar}dfkmgpar ma Defck}appk# }pk}kpge mdyaprkr kpbdcckr bge rqcik}g ma kngedg g bkp`gek}g ae rgcqbdòe zkpk ax}pkap ac kngeîkbg t cd`apkp bkcbdg% Ae ;406# ck zpdnapk bgcqnek ma |agcd}k ndeapkc iqa q}dcd|kmk zkpk acdndekp dgear ma bkcbdg t nkfeardg {qa de}apiapîke ae ck rgcqbdòe# zkpk ma}apndekp ac bge}aedmg ma rqcik}g ma kfqk% Ck yaprdòe ngmapek ma ck DAB ra markppgccò mqpke}a ck äzgbk ma ck fqappk mac Zpgtab}g Nkehk}}ke% Apk eabarkpdk qek }äbedbk zkpk razkpkp t bgebae}pkp cgr acanae}gr pkmdkb}dygr eabarkpdgr zkpk hkbap ck `gn`k k}òndbk% Cgr deyar}dfkmgpar acdfdapge k`rgp`ae}ar {qa zqmdapke bappkpra rg`pa acanae}gr }pkerqpëedmgr bkpfkmgr# t {qa zqmdapke rap acdndekmgr mdiapaebdkcnae}a% Üc}dnknae}a# qek ya| marbckrdidbkmg# ar}kr }äbedbkr zqamae q}dcd|kp eqaykr pardekr DA zkpk markppgcckp cgr rdr}ankr {qa ra q}dcd|ke k naeqmg hgt ae mîk zkpk ck zqpdidbkbdòe arzabîidbk ma zpgmqb}gr `dgcòfdbgr t ma rqr}kebdkr degpfëedbkr% K zpdebdzdgr ma cgr &6:# ck bpgnk}gfpkiîk dòedbk# iqa markppgcckmk zgp Hkndrh Rnkcc t rqr bgnzkûapgr ma }pk`klg ae Mgv Bhandbkc Bgnzket bgng qe egyamgrg nä}gmg ma DAB q}dcd|k`car ae ac keëcdrdr kq}gnk}d|kmg% BD q}dcd|k pardekr dòedbkr nër mä`dcar zkpk rq ikra ar}kbdgekpdk t qek eqayk eaq}pkcd|kbdòe ma r}pdzzap # g rqzpargp ma ck bgcqnek zkpk acdndekp cgr dgear {qd}kmgr {qa ra mazgrd}ke ae ac igemg% Ar qek zgmapgrk }äbedbk zkpk ma}apndekp `klkr bgebae}pkbdgear ma dgear t ar arzabdkcnae}a ü}dc ae ar}qmdgr rg`pa ac namdg kn`dae}a t ck bkcdmkm mac kfqk# ae}pa g}pkr kzcdbkbdgear%

]äbedbk

Ck pakcd|kbdòe ma ar}k }äbedbk ra hkba q}dcd|kemg bgcqnekr paccaekr ma qe maegndekmg de}apbkn`dkmgp dgedbg (rqr}kebdk {qa bge}daea fpqzgr bkpfkmgr bge dgear nòydcar ma rdfeg bge}pkpdg$% Cgr de}apbkn`dkmgpar zqamae rap kedgedbgr (de}apbkn`dke kedgear bge ac namdg$ g bk}dòedbgr (de}apbkn`dke bk}dgear bge ac namdg$% K }pkyär ma ck bgcqnek hkbangr zkrkp qek mdrgcqbdòe ma rqr}kebdkr dgedkmkr% K rq zkrg zgp ck bgcqnek# ckr mdiapae}ar rqr}kebdkr {qamke qedmkr kc de}apbkn`dkmgp dgedbg napbam k iqap|kr acab}pgr}ë}dbkr% Marzqär hkbangr zkrkp zgp ck bgcqnek }knzgear ma mdiapae}a zH# {qa zpgygbk bkn`dgr ae ckr iqap|kr acab}pgr}ë}dbkr% Zgp bkmk }knzòe q}dcd|kmg bgerafqdngr ck razkpkbdòe ma ck bgcqnek# zgp acqbdòe# ma qek ma}apndekmk rqr}kebdk dged|kmk%

A{qdzg zkpk bpgnk}gfpkiîk ma de}apbkn`dg dòedbg%

Der}pqnae}kbdòe< Qe a{qdzg zkpk keëcdrdr namdke}a bpgnk}gfpkiîk ma de}apbkn`dg dòedbg (B%D%D%$ ar}ë bger}d}qdmg `ërdbknae}a zgp cgr bgnzgeae}ar rdfqdae}ar<         

Qe detab}gp  Qek yëcyqck zkpk ck detabbdòe Qe bgnzkp}dndae}g zkpk ck bgcqnek Qek g ykpdkr bgcqnekr Qek `gn`k qe rdr}ank ma ma}abbdòe bgemqb}dnä}pdbg Qe rdr}ank ma pafdr}pg g ma de}afpkbdòe Qe kq}g nqar}pakmgp  Qe ndbpgzpgbarkmgp zkpk kq}gnk}d|kp gzapkbdgear Qe bgnzq}kmgp zkpk nkealkp t kcnkbaekp deigpnkbdòe%

Ac mdraûg ma qe a{qdzg bge ar}kr bkpkb}apîr}dbkr# zapnd}a kzcdbkbdgear kekcî}dbkr bqkcd}k}dykr t bqke}d}k}dykr pëzdmkr# racab}dykr t rdnqc}ëeakr zpdebdzkcnae}a ma arzabdar dòedbkr degpfëedbkr ae ac pkefg ma ndbpgfpkngr zgp cd}pg ae nqar}pkr ma kfqkr# bge}kndeke}ar kn`dae}kcar# demqr}pdk ma kcdnae}gr# `a`dmkr# zpgmqb}gr ikpnkbäq}dbgr# pabq`pdndae}gr# zpgmqb}gr kfpîbgckr# ck`gpk}gpdgr bcîedbgr# ck`gpk}gpdgr ma bge}pgc ma bkcdmkm t keëcdrdr ae faeapkc% Ckr ma}apndekbdgear ma kedgear t bk}dgear nër ipabqae}ar rge< Bcgpqpgr# `pgnqpgr# icqgpqpgr# ed}pd}gr# 02ed}pk}gr # rqcik}gr# igrik}gr# `gpk}gr# rqcigbdkeqpg# rdcdbk}gr# cd}dg# rgmdg# zg}krdg# bkcbdg# nkfeardg# ar}pgebdg# `kpdg# |deb# bg`kc}g# nkefkearg# kngedg# ëbdmgr bkp`gxîcdbgr (}kp}pk}gr# ckb}k}g# kba}k}g$ t kndekr (na}dc kndek# mdna}dc kndek# }pdna}dc kndek

%$ACANAE]GR @KRDBGR< 

Ikra ar}kbdgekpdk ( Idlk $

Ikra Ar}kbdgekpdk< Dergcq`ca# ccayk ae ck rqzapidbda bkpfkr acab}pgr}ë}dbkr idlkr {qa

pa}daeae bge}pkdgear nòydcar {qa zqamae de}apbkn`dkpra zgp dgear ma ck ikra nòydc% Ck ikra ar}kbdgekpdk ar egpnkcnae}a qek pardek ma de}apbkn`dg dòedbg {qa bge}daea fpqzgr iqebdgekcar bkpfkmgr {qa de}apkbbdgeke bge fpqzgr bkpfkmgr ma rdfeg gzqar}g mac bgnzqar}g {qa ra {qdapa pa}aeap% Zqama rap< ;% De}apbkn`dkmgp ma dgear bkpfkmg zgrd}dyknae}a (de}apbkn`dkmgp ma kedgear$# {qa de}apkbbdgek bge kedgear 0% De}apbkn`dkmgp ma dgear bkpfkmg eafk}dyknae}a (de}apbkn`dkmgp ma bk}dgear$# {qa de}apkbbdgek bge bk}dgear

Pardekr

D% Pardekr ma zgcdar}dpaeg ae}pabpq|kmkr DD% Pardekr `krkmkr ae Bkp`ghdmpk}gr. (Bkp`ghdmpk}gr Zgcdnäpdbgr# Kfkpgrk# max}pkeg# bacqcgrk$ Arzabdkcnae}a qrkmgr ae ck razkpkbdòe ma `dgngcabqckr% DDD% Rgzgp}ar `krkmgr ae ck Rîcdba

]dzg ma pardekr qrkmkr



Ikra nòydc ( Ra marzck|k $

Ikra Nòydc< Mdrgcqbdòe kbqgrk bge bke}dmkmar ngmapkmkr ma na}kegc q g}pg mdrgcyae}a

gpfëedbg ndrbd`ca bge kfqk {qa bge}daea arzabdar dòedbkr ae igpnk# faeapkcnae}a ma `qiiap% Cgr dgear ma ck ikra nòydc bgnzd}ae bge cgr keëcd}gr zgp cgr rd}dgr kb}dygr ma ck ikra ar}kbdgekpdk% ]knzgear

Zkpk ar}gr de}apbkn`dkmgpar ra rqacae qrkp% Hdr}dmdek (zOk 9#;1$# dndmk|gc (6#:$# }pda}kegckndek (6#66$# ]pdr (8#;9$# mda}kegckndek (8#8$ t g}pgr% Zgp ck ndrnk pk|òe# zkpk ck bp% d% bk}dòedbg ra ma`a qrkp qe }knzòe kedòedbg# bgng kbä}dbg (zOk =#69$# bî}pdbg (=#69$# Nar (9#;1$# igrik}g (6#0$# Hazar (6#11$ t g}pgr% Acabbdòe mac }knzòe t ck iqap|k dòedbk%

 Kzkp}a mac zH ma ck ikra ngydc# ae ck zqpdidbkbdge ma qe rgcq}g'zpg}adek hkt {qa }aeap ae bqae}k ac }dzg ma }knzòe {qa ra qrk t ck iqap|k dòedbk g bgebae}pkbdge rkcdek ae ck {qa }daea cqfkp ck de}apkbbdge bge ac de}apbkn`dkmgp% Parzab}g kc }dzg ma }knzge# ck igpnk bkpfkmk mac ndrng eg ma`a de}apiapdp bge ac zpgbarg ma de}apbkn`dg ma dgear# zgp cg {qa eg ma`a kb}qkp bgng bge}pkdge t bgnza}dp bge ck zpg}adek zgp ck qedge k ck i% ar}kbdgekpdk# zgp{qa accg marydp}qkpdk rq a{qdcd`pdg (t kmankr ra bpakpdk qe fpkmdae}a ma zH ae ck bgcqnek$% Zkpk accg# ac bgnzgeae}a dòedbg mac }knzòe ma`a ma }aeap ck ndrnk bkpfk {qa ck ikra ar}kbdgekpdk% A}kzkr mac zpgbarg bpgnk}gfpkidbg

;% Ck zpg}aîek {qa bge}daea ae nktgp zgpbae}kla bkpfkr eafk}dykr# ra ya pgmakmk zgp dgear zgrd}dygr% Ar}k zpg}aîek de}apkbbdgekpë bge qek nk}pd| {qa zgrak bkpfkr zgrd}dykr% 0% Ra zpgmqbae de}apkbbdgear acab}pgar}ë}dbkr ae}pa ck zpg}aîek t ck nk}pd|# zgp cg bqkc ra marzck|ke cgr dgear zgrd}dygr t eafk}dygr {qa ra aebge}pk`ke de}apkbbdgekemg }ke}g bge ck zpg}aîek bgng bge ck nk}pd|% 2% Qek ya| {qa ck zpg}aîek ar kmrgp`dmk ae ck nk}pd| ra ckyk ck bgcqnek bge qe `qiiap  {qa zapnd}k acdndekp cgr bge}knd.eke}ar {qa eg ar}ëe kmrgp`dmgr% =% Zgr}apdgpnae}a ra acqta ck zpg}aîek kmrgp`dmk ae igpnk mdiapaebdkc namdke}a bkn`dgr< . ma iqap|k dòedbk . zH ma ck rgcqbdòe acqtae}a 1% Idekcnae}a ra pakbgemdbdgek ck nk}pd| zkpk dedbdkp qe eqayg bdbcg% Mazaemdaemg mac nk}apdkc ra zqamae pakcd|kp hkr}k ;:: bdbcgr%

Yae}klkr'Maryae}klkr Yae}klkr<   



Kc}k bkzkbdmkm Kc}k pargcqbdòe% Zkrg bgebae}pkmgp< Ra zqama ran`pkp qe fpke ygcqnae zkpk cqafg acqdpcg ae qe naegp ygcqnae% Bge qek ndrnk bgcqnek ra zqama q}dcd|kp k mdiapae}ar zH t g`}aeap mdyaprgr zapidcar ma acqbdòe%

Maryae}klkr< 

Ckr nqar}pkr ma`ae ar}kp markcded|kmkr ke}ar ma rap kzcdbkmkr k ar}a }dzg ma bpgnk}gfpkiîk

Kzcdbkbdgear

Bgng kzcdbkbdgear ma ck bpgnk}gfpkiîk dòedbk ar}ë ac keëcdrdr ma mpgfkr t rqr na}k`gcd}gr# rqapgr# bgerapyke}ar ma kcdnae}gr# na|bckr zkpk yd}kndekr# k|übkpar# t zpazkpkbdgear ikpnkbäq}dbkr% Ac de}apbkn`dg dòedbg ra q}dcd|k knzcdknae}a ae ckr demqr}pdkr ma kcdnae}gr t `a`dmkr# hdmpgna}kcüpfdbk# kbk`kmg ma na}kcar# {qîndbk t za}pg{qîndbk# ikpnkbäq}dbk# k|übkp t amqcbgpke}ar# kfqk rq`}appëeak t zg}k`ca# eqbcakp# k`ckemkndae}g demqr}pdkc mac kfqk# randbgemqb}gpar# aeapfîk# t g}pkr nqbhkr demqr}pdkr% Ae `dg{qîndbk ar knzcdknae}a q}dcd|kmg zkpk razkpkp ngcäbqckr bkpfkmkr# }kcar bgng zpg}aîekr% Qe ëpak dnzgp}ke}a ma kzcdbkbdòe ar ck ax}pkbbdòe t zqpdidbkbdòe ma rqr}kebdkr ma gpdfae `dgcòfdbg# }kcar bgng zpg}aîekr (kndegëbdmgr$ t KME'KPE% Qe alanzcg nqt dnzgp}ke}a ar ac zpgbarg ZQPAX (Zcq}gedqn.QPkedqn AX}pkb}dge zpgbarr# zpgbarg ma ax}pkbbdòe ma zcq}gedg.qpkedg$ {qa ra q}dcd|k zkpk razkpkp ac zcq}gedg t acqpkedg ae}pa cgr zpgmqb}gr zparae}ar ae ac bgn`qr}d`ca fkr}kmg ma qe pakb}gp eqbcakp# t zgmap acdndekp cgr zpgmqb}gr ma marabhg% Ma ar}a ngmg# ac zcq}gedg t ac qpkedg ar}ëe mdrzged`car zkpk rap anzcakmgr bgng nk}apdkcar packbdgekmgr bge ck aeapfîk eqbcakp# bgng eqayg bgn`qr}d`ca ma pakb}gp t kpnkr eqbcakpar% Ra q}dcd|k }kn`däe zkpk razkpkp g}pgr bgelqe}gr ma acanae}gr {qîndbgr nqt rdndckpar# }kcar bgng bdpbgedg t hkiedg# {qa zgp bdap}g rge }kn`däe nqt dnzgp}ke}ar zkpk ck demqr}pdk eqbcakp% Ac bdpbgedg ar zpëb}dbknae}a }pkerzkpae}a k cgr eaq}pgear cd`par# t ra q}dcd|k ae ck bger}pqbbdòe ma pakb}gpar# zapg ac hkiedg ar qe k`rgp`ae}a ma eaq}pgear nqt iqap}a# qrkmg ae ckr `kppkr ma bge}pgc mac pakb}gp% Ra q}dcd|ke ae ac pazpgbarkndae}g mac bgn`qr}d`ca eqbcakp t ac }pk}kndae}g ma cgr pardmqgr pkmdkb}dygr%

G}pkr kzcdbkbdgear

Ae bdaebdk mac rqacg# ck bkzkbdmkm ma de}apbkn`dg bk}dòedbg ar ck bkzkbdmkm ma de}apbkn`dg dòedbg ma cgr rqacgr zkpk cgr dgear ma bkpfk zgrd}dyk% Cgr rqacgr zqamae rap  bgerdmapkmgr bgng de}apbkn`dkmgpar ek}qpkcar ma bk}dgear mä`dcar% Ae ck ik`pdbkbdòe ma fqîkr ma gemk zckekr# ac de}apbkn`dg dòedbg ra q}dcd|k zkpk bpakp ck bkzk fqîk ma îemdba ma paipkbbdòe rqzapdgp% Markcbkcded|kbdòe# g acdndekbdòe ma cgr dgear kcbkcdegr ma ck rqzapidbda ma qe ydmpdg% Zpgmqbbdòe ma Ydmpdg aemqpabdmg {qîndbknae}a# zpgmqbdmg zgp ac de}apbkn`dg ma dgear Ek/ zgp O/ ae ckr rqzapidbdar ma bpdr}kc qrkemg OEG2 iqemdmg% Ax}pkbbdòe t zqpdidbkbdòe ma ëbdmgr eqbcadbgr Zqpdidbkbdòe ma kfqk Zqpdidbkbdòe mac id`pdeòfaeg ma ck cabha Ma}apndekbdòe ma ke}dbgeyqcrke}ar ae ac rqapg marzqär ma ck ax}pkbbdòe ma ck ikra ròcdmk%  Keëcdrdr ma ke}d`dò}dbgr ma zgcd ä}ap% Ma}apndekbdòe ma zar}dbdmkr% Dmae}didbkbdòe ma ëbdmgr gpfëedbgr ae ck ipq}k t ae ac lqfg% Ma}abbdòe ma mapdykbdòe.icqgparbaebdk zgr}.bgcqnek zkpk keëcdrdr zkpk ykpdgr }dzgr ma zar}dbdmkr kfpîbgckr% Ma}apndekbdòe ma na}k`gcd}gr ma ]pdz}òikeg ae ac rqapg qn`dcdbkc%

Alanzcg