UNIVERSIDAD DE EL SALVADOR FACULTAD DE QUIMICA Y FARMACIA
“OBTENCION, REFINACION Y CARACTERIZACION DEL ACEITE DE LA SEMILLA DE Passiflora edulis flavicarpa (MARACUYA)”. TRABAJO DE GRADUACION PRESENTADO POR: ROXANA LIZET CRUZ CLAUDIA LISSETTE MELENDEZ ZEPEDA PARA OPTAR AL GRADO DE LICENCIATURA LICENCIATURA EN QUIMICA Y FARMACIA
FEBRERO DE !""# SAN SALVADOR. EL SALVADOR. CENTRO AMERICA CENTRO AMERICA
UNIVERSIDAD DE EL SALVADOR
RECTORA DRA. MARIA ISABEL RODRIGUEZ
SECRETARIA GENERAL LICDA. LIDIA MARGARITA MU$OZ VELA
FACULTAD DE QUIMICA Y FARMACIA
DECANO LIC. SALVADOR CASTILLO AREVALO CASTILLO AREVALO
SECRETARIA
UNIVERSIDAD DE EL SALVADOR
RECTORA DRA. MARIA ISABEL RODRIGUEZ
SECRETARIA GENERAL LICDA. LIDIA MARGARITA MU$OZ VELA
FACULTAD DE QUIMICA Y FARMACIA
DECANO LIC. SALVADOR CASTILLO AREVALO CASTILLO AREVALO
SECRETARIA
MS%. MIRIAM DEL CARMEN RAMOS DE AGUILAR DE AGUILAR
COORDINADORA GENERAL LICDA. MARIA C. ODETTE RAUDA ACEVEDO RAUDA ACEVEDO
COORDINADORA DE AREA BIOQUIMICA Y CONTAMINACION AMBIENTAL CONTAMINACION AMBIENTAL MS%. SONIA MARICELA LEMUS
COORDINADORA DE AREA DE AREA GESTION AMBIENTAL, TOXICOLOGIA Y QUIMICA LEGAL LICDA. MARIA LUISA ORTIZ DE LOPEZ
DOCENTE DIRECTOR ING. SERGIO ARMANDO MARAVILLA MIRANDA
AGRADECIMIENTOS
•
A D&': '* +*&-&*+ %'*'/* & %/**+*/ *'0+&'/1.
•
A & /2*+: '* 3*&2/*+ 4 A'* 5 %'*+&6 + %/2/ +742' 2+ & 8&2/.
•
A & 0/&1&/: '* 4 /'5' &%'2&%&'/1.
•
•
•
A & %'/9+*/ C1/42&/ M+12+; '* 4 7*/ -'1+*/%&/. A 1/ 0/&1&/ M+12+; Z++2/ '*
•
A 4+-*' /&7' L&%. M/*%' A-'&' A=4&' 5 L&%. R+ A1+>/2+* E%/1/-+ '*
•
A1 S*. L4& A1'' A3*+7' 5 S*. V@%-'* S%<+; '* 4 +*8&%&/1&2/2 + +1 2+/**'11' 2+ 1/ /*-+ +>+*&+-/1.
•
A D'*& +*@=4+; '* -'2/ 1/ /542/ 3*&2/2/ 2+&-+*+/2/+-+ + 1/ *+/1&;/%&6 2+ +-+ -*/3/?'.
•
A 4+-*' /+'*+ 2+ *+/ L&%. O2+--+ R/42/ A%+8+2', MS%. M/*&+1/ L+4 5 L&%. M/*&/ L4&/ 2+ L6+;, '*=4+ 8/1&2'' 2+ 4 +>+*&+%&/ *'0+&'/1 <+' '2&2' -+*&/* %' >&-' +1 *++-+ -*/3/?'.
•
A -'2/ 1/ +*'/ =4+ 2+ 4/ 4 '-*/ 0'*/ %'1/3'*/*' + 1/ *+/1&;/%&6 2+ +-+ -*/3/?'.
R'>// L&;+-< C*4;
AGRADECIMIENTOS
A D&': P'*
A & /2*+: J' R/0/+1 M+12+; 5 N+115 Z++2/ 2+ M+12+; '*
A & <+*//: C'*/1&/ 5 N+115 M+12+; Z++2/, '* 4 +?+1' 2+ <+*/2/2, %/*&9' 5 '1&2/*&2/2.
A 4+-*' /+'*: I7. S+*7&' A*/2' M/*/8&11/ '* 3*&2/* 4 %''%&&+-' 5 2+2&%/%&6 + +1 2+/**'11' 2+ +-+ -*/3/?'.
A & %'/9+*/: R'>// L&;+-< C*4; '* 4 /&-/2 &%+*/ 5 4 &+' /'5' + 1/ *+/1&;/%&6 2+ +-+ -*/3/?'.
A D'*& +*@=4+;: D+ /+*/ 45 ++%&/1 '* 4 /&-/2 5 /'5' 3*&2/2' 2+ /+*/ 2+&-+*+/2/.
C1/42&/ L&+--+ M+12+; Z++2/
DEDICATORIA
•
A D&': '*
•
A & /2*+: M&*&/ C'%+%&6 C*4;, '* +* 1/ &&*/%&6 7*/2+ /*/ 11+7/*
•
A & /34+1&-/: M/*@/ 2+ C*4;, '* +* +1 ''*-+ +&*&-4/1 =4+ + '-&++ / 1' 1/*7' 2+ & 8&2/.
•
•
A & -@', -@/ 5 *&/ '* '-&8/*+ / +74&* &+*+ /2+1/-+.
A & /&7/: F-&/, P/-<5, S/*/ 5 M/*%+1/ '* +-/* %'&7' + -'2' '+-'.
•
A & %'/9+*/: C1/42&/ M+12+; '* 2+'-*/*+ +1 8+*2/2+*' 8/1'* 2+ 1/ /&-/2 5
•
A & <+*/&-/: A7+1/ I8/&/ '* 4 '1&2/*&2/2 + -'2' 1' '+-' =4+ 1/ <+ +%+&-/2'. R'>// L&;+-< C*4;
DEDICATORIA
A D&': P'*
A & /2*+: J' R/0/+1 M+12+; 5 N+115 Z++2/ 2+ M+12+; %' -'2' & /'* 5 7*/-&-42, '* 1' +04+*;' 5 /%*&0&%&' =4+ <&%&+*' /*/ '2+* /1%/;/* +-+ -*&40'.
A & A34+1': R/0/+1 A-'&' Z++2/ O*/-+ (Q.D.D.G) 2+ /+*/ 45 ++%&/1, =4&+ 04+ & +?+1' 2+ +*+8+*/%&/ 5 4+*/%&6 /%/2&%/.
A & 0/&1&/: %' 4%<' %/*&9' '* 4 /'5' 5 '-&8/%&6 + %/2/ '+-' 2+ & 8&2/.
C1/42&/ L&+--+ M+12+; Z++2/
INDICE
P/7 >&&
I. INTRODUCCIN II. OBJETIVOS III. MARCO TERICO . G++*/1&2/2+ 2+ Passiflora edulis flavicarpa (/*/%45)
.. C1/&0&%/%&6 T/>'6&%/
..! V/*&+2/2+
.. C''&%&6 Q4@&%/
..# C'+%
.. E>&7+%&/ 2+1 C41-&8'
.. U'
H
.! G++*/1&2/2+ 2+ /%+&-+ 8+7+-/1+
.!. C''&%&6 Q4@&%/ 2+ A%+&-+ V+7+-/1+
.!.! E>-*/%%&6 2+ A%+&-+ V+7+-/1+
!"
.!.!. E>-*/%%&6 2+1 A%+&-+ '* P*+/2'
!"
.!.!.! E>-*/%%&6 %' S'18+-+
!"
. R+0&/%&6 2+ A%+&-+ V+7+-/1+
!
.# A1&& F&&%'=4@&%' 2+ A%+&-+
!!
.#. P*4+3/ F@&%/
!!
.#.! P*4+3/ Q4@&%/
!
. D+-+*&/%&6 2+ P*'-+@/ IV DISE$O METODOLGICO
! !
#. I8+-&7/%&6 B&31&'7*0&%/
!
#.! I8+-&7/%&6 2+ C/'
!
#.!. L'%/1&;/%&6 5 R+%'1+%%&6 2+ M/-+*&/ P*&/
!
#.!.! T*/-/&+-' 2+ S+&11/
!
#.!. T*&-4*/%&6 2+ S+&11/
!
#. P/*-+ E>+*&+-/1
!
#.. E>-*/%%&6 2+1 A%+&-+
!
#..! R+0&/%&6 2+1 A%+&-+
!H
#..!.R+0&/%&6 Q4@&%/
!H
#..!.! R+0&/%&6 F@&%/
!
#.. A1/%+/&+-'
"
#..# A1&& F&&%'=4@&%' 2+1 A%+&-+
"
#..#. P*4+3/ F@&%/
"
#..#.! P*4+3/ Q4@&%/
#
#.. C4/-&0&%/%&6 2+ P*'-+@/ 2+ 1/ S+&11/
H
V
RESULTADOS
#
VI
DISCUSION DE RESULTADOS
#
VII CONCLUSIONES
VIII RECOMENDACIONES
BIBLIOGRAFA ANEXOS
INDICE DE CUADROS DE RESULTADOS
C4/2*'
P7.
. R+2&&+-' P'*%+-4/1 2+1 /%+&-+
#
!. R+0&/%&6 Q4@&%/ 5 F@&%/
#!
. D+-+*&/%&6 2+
#!
#. D+-+*&/%&6 2+ P4-' 2+ 4'
#!
. D+-+*&/%&6 2+ V&%'&2/2
#
. D+-+*&/%&6 2+ D+&2/2
#
. D+-+*&/%&6 2+ C+&;/
#
H. D+-+*&/%&6 2+ 2&%+ 2+ R+0*/%%&6
##
. D+-+*&/%&6 2+ S'143&1&2/2
##
". D+-+*&/%&6 2+ 2&%+ 2+ A%&2+;
#
. D+-+*&/%&6 2+ 2&%+ 2+ Y'2'
#
!. D+-+*&/%&6 2+ 2&%+ P+*'>&2'
#
. D+-+*&/%&6 2+ @2&%+ 2+ S/'&0&%/%&6
#
#. C4/-&0&%/%&6 2+ P*'-+@/
#
INDICE DE ANEXOS A+>' F&74*/ NK : M/*/%45 /-+ 2+ +* 1/-/2/
NK !: P1/-/%&6 2+ M/*/%45
NK : P1/-/ 2+ M/*/%45
NK #: F*4-' 2+ M/*/%45
NK : S+&11/ 2+1 F*4-'
NK : E=4&' 2+ E>-*/%%&6 S'><1+-
T/31/ NK : S'143&1&2/2 USP !#
!
NK !: P*4+3/ Q4@&%/ 2+ A%+&-+ 2+ V+7+-/1+
!
NK : P*4+3/ F@&%/ 2+ A%+&-+ V+7+-/1+
!
NK #: P'*%+-/?+ 2+ P*'-+@/ + A%+&-+ V+7+-/1+
!
R+41-/2' 2+ C4/-&0&%/%&6 2+ P*'-+@/
P*'%+' I24-*&/1 2+ R+0&/%&6 + E1 D'*/2'
#
M/-+*&/1 5 E=4&'
C1%41' 2+ P*4+3/ Q4@&%/ 5 F@&%/
xii
1.0 INTRODUCCIÓN
E-*+ 1/ ++%&+ 8+7+-/1+ 2+ 4' ' -*/2&%&'/1 + +%4+-*/ 1/ Passiflora edulis flavicarpa %45' '3*+ %' + “M/*/%45”, &+2' +1 0*4-' 1/ /*-+ / 4-&1&;/2/
2+ +-/ 1/-/ *&%&/1+-+ + 1/ &24-*&/ /1&+-&%&/ ++%@0&%/+-+ + *+0*+%', & 2/*1+ &7 /*'8+%-*/%%&6, *+0&/%&6 5 %/*/%-+*&;/%&6 2+1 /%+&-+ '3-+&2' 2+ 1/ +&11/ 2+ Pasiflora edulis flavicarpa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
2.0 OBJETIVOS
2.1 Objet!" Ge#e$%& O3-++*, *+0&/* 5 %/*/%-+*&;/* +1 /%+&-+ 2+ 1/ +&11/ 2+ Passiflora edulis flavicarpa (M/*/%45).
2.2 Objet!"' E'(e)*+)"' !.!. E>-*/+* +1 /%+&-+ 2+ 1/ +&11/ 2+ M/*/%45 4-&1&;/2' <+>/'. !.!.! C4/-&0&%/* +1 *+2&&+-' '*%+-4/1 2+1 /%+&-+ +>-*/@2'. !.!. R+/1&;/*
*+0&/%&6 0@&%/ 5 =4@&%/ 2+1 /%+&-+ '3-+&2' +2&/-+ 1'
-*/-/&+-'
7++*/1+
2+
4*&0&%/%&6:
2+7'/2',
+4-*/1&;/%&6,
2+'2'*&;/%&6 5 &-+*&;/%&6. !.!.#
R+/1&;/* /1&& 0&&%'=4@&%' 5 %'-*'1+ 2+ %/1&2/2 /1 /%+&-+ '3-+&2' %'' *'24%-' -+*&/2'.
!.!. C4/-&0&%/* +1 %'-+&2' 2+ *'-+@/ 2+1 *+&24' 2+ 1/ +&11/ 2+ Passiflora edulis flavicarpa (M/*/%45).
CA,ITULO III MARCO TEÓRICO
15
-.0 MARCO TEORICO
-.1 GENERALIDADES DE Passiflora edulis flavicarpa MARACUYA/. E1 M/*/%45, + '*&7&/*&' 2+1 T*/+%&' A/;6&%', /%-4/1+-+ + %41-&8/ + B*/&1, =4+ + +1 /5'* +>'*-/2'* 42&/1 2+ ?47'. E1 M/*/%45 '++ 4 /3'* /*-&%41/* &-+' 5 4 /1-/ /%&2+;, %'-&-452'+ + 4/ 3/+ 04+*-+ /*/ 3+3&2/ &24-*&/1&;/2/. A@ &', +-/ ++%&+ + 34+/ 04+-+ 2+ 8&-/&/ A 5 &/%&/.
-.1.1 C&%'+)%)# t%"#)% 3e Passiflora edulis flavicarpa M%$%)456/. 7 Re#"8 V+7+-/1 T$"#)": C'*60&-/ D!'#: A-'0&-/ S4b93!'#8 A7&'+*/ C&%'e8 D&%'-&1+26+/ S4b9)&%'e8 D&/1&-/1/ O$3e#: V&'1/1+ F%&%8 P/&01'*/%+/+ Ge#e$"8 P/&01'*/ E'(e)e8 +241&
16
-.1.2 V%$e3%3e' E>&-+ 2' 8/*&+2/2+ ' 0'*/ 2+ Passiflora edulis flavicarpa ( M/*/%45 ): M /* /%4 5 A/*&11' ( Passiflora edulis variedad flavicarpa )
=4+ *++-/ 0*4-' 8&-'' 2+ %'1'* //*&11'
%' 2&8+*/ 0'*/. E-/ 8/*&+2/2 %*+%+ 5 + 2+/**'11/ 45 3&+ + ;'/ 3/?/. E 4/ 1/-/ *-&%/ 5 8&7'*'/ =4+ +1 M/*/%45 *4*/.
M/*/%45 *' ?' ' '*/2' (Passiflora edulis variedad púrpura Sims) =4+ *++-/ 0*4-' +=4+9' 2+ %'1'* *'?'.
E-/ 8/*&+2/2 %*+%+ 5 + 2+/**'11/ + ;'/ -+1/2/.
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
17
-.1.: C"'e);% El Maracuyá es una planta fructífera que comienza a producir en el primer año de sembrado, corta.
además
tiene
un
período
de
vida
relativamente
El mayor rendimiento se obtiene en el segundo o tercer año y
disminuye en los años siguientes. 15
E1 4-' 2+ /24*+; 0&&'167&%/ +- 2/2' '* +1 2+*+2&&+-' 2+ 1/ 0*4-/ 2+ 1/ 1/-/, %/5+2' /1 4+1' 5 /11@ + 2'2+ +
-.1.7 E
18
3) R+=4+*&&+-' +20&%'. T&' 2+ 4+1': F*/%' /*%&11'/*+'', /&*+/2' 2+ 0%&1 2*+/?+, /1-' + /-+*&/ '*7&%/. A%&2+;: #..
-.1.= U'"' E1 0*4-' + %'4+ %'' 0*4-/ 0*+%/ ' + ?47'. S+ 4-&1&;/ /*/ *+/*/* *+0*+%', %-/*+, 5'74*+, +*+1/2/, 1&%'*+, <+1/2', +1/-/2'. S+ *+%'&+2/ /*/ 3/?/* 1/ *+&6 /*-+*&/1, %'' -*/=4&1&;/-+ 5 %'' 04+-+ 2+ 8&-/&/ C.
L/ +&11/ 2+ /*/%45 + 4&&-*/ +-+*/ / 1/ 7/11&/ 5 / 1' %+*2' + 1+ 2 '1&2/ %'' 04+-+ 2+ 7*// 5 *'-+@/. E1 /%+&-+ 2+ 1/ +&11/ 2+ /*/%45 + 4-&1&;/ + 1/ *+/*/%&6 2+ %&+*-' *'24%-' %'-&%', 2+3&2' / =4+ +-+ *++-/ 4/ *&%/ %''&%&6 2+ %&2' 7*/', /542/2' / 1/ *+-/4*/%&6 2+ 1/ %// 1&@2&%/ 2+ 1/ &+1 5 8'18&2'1/ +'1&+-+ / -*/8 2+ 1/ 0'*/%&6 2+ 4/ +1@%41/, 2+?/2' 1/ &+1 +2'/. S+ 4-&1&;/ 1/ /-+%/ 2+ 1/ +&11/ + =4+/24*/, /*/ /1/%/* 2'1'*+ 5 -/3& %'' <&2*/-/-+, 2+3&2' / 4 /1-/ %//%&2/2 2+ /3'*%&6 2+ /74/, 1' =4+ '&3&1&-/ 1/ *+%4+*/%&6 2+ 1/ <4+2/2 /-4*/1 2+ 1/ &+1. A%-/ '3*+ +1 -+?&2' %4-+' 0'*/2' 4/ +1@%41/ *'-+%-'*/ =4+ &&2+ 1/ +8/'*/%&6 2+ /74/ + 1/ &+1.H
19
-.2 GENERALIDADES DE ACEITES VEGETALES. L' /%+&-+ 5 1/ 7*//, %4/1=4&+*/ =4+ +/ 4 '*&7+ (8+7+-/1 ' /&/1), ' 4-/%&/ 1&=4&2/ ' 61&2/, ' +' %'1'*+/2/, 4-4'/, &01//31+, 04&31+, 1&8&// + &'1431+ + /74/ 5 + /1%'<'1, %'-&-4&2/ '* %/*3'', <&2*67+' 5 '>@7+'. E-+ 1-&' +1++-' ' + +%4+-*/ + 1' /%+&-+ 5 7*// &+*/1+. L' /%+&-+ 5 1/ 7*// 8+7+-/1+ + +%4+-*/ + /5'*+ *''*%&'+ + 1/ +&11/ 5 + 1' 0*4-'. A174' + +>-*/+ 2+ 1/ */@%+, /4=4+ +-' +
-.2.1. C"("')# >4*)% 3e %)ete' !e-*/%%&6 5 R+0&/%&6.
20
-.2.2 Et$%))# 3e %)ete' !e-*/+* '* 2' *'%+2&&+-' 3&+ 2&0+*+%&/2': /) '* *+/2', 5 3) %' 2&'18+-+.
-.2.2.1. Et$%))# 3e %)ete' ("$ ($e#'%3" P/*/ +/*/* +1 /%+&-+ 2+ 1/ +&11/ 4+2+ +1+/*+ *+/ +%&%/ ' <&2*41&%/, =4+ / 4 8+; 4+2+ +* %'-&4/ ' 2&%'-&4/. N' %/3+ 242/ =4+ 1/ *+/ <&2*41&%/ 2&%'-&4/ ' 1/ *%-&%/ 5 +0&%&+-+.H
-.2.2.2 Et$%))# )"# '"&!e#te' E1 *'%+' 2+ +>-*/%%&6 2+ /%+&-+ %' '18+-+ +*&-+ +1 /7'-/&+-' %/& -'-/1 2+ 1/ +&11/. E-+ *'%+' %'&-+ + -*/-/* 1/ +&11/ %' 4 '18+-+ 8'1-&1 =4+ 7++*/1+-+ + +1 <+>/', +1 %4/1 + %//; 2+ 2&'18+* 7*/2+ %/-&2/2+ 2+ /%+&-+ & +>-*/+* '-*' %'4+-' *++-+ + +-+. P'-+*&'*+-+ / -*/8 2+ 4 *'%+' 2+ 2+-&1/%&6 + *+-&*/ -'2' +1 '18+-+ 4-&1&;/2', %'2+2'1' /*/ 4 *+4-&1&;/%&6.
21
-.- REFINACION DE ACEITES VEGETALES L' /%+&-+ '3-+&2' '* 1' -'2' /-+ +%&'/2' 2+3+ '+-+*+, 4+, / %&+*-' -*/-/&+-' 2+ 4*&0&%/%&6 5 *+0&/%&6, %' 1' %4/1+ + +1&&/ -'2/ /=4+11/ %/*/%-+*@-&%/ =4+ /0+%-/ 4 %/1&2/2. L' -*/-/&+-' 7++*/1+ 2+ *+0&/%&6 =4+ + /1&%/ %'+-+ / 1' /%+&-+ 8+7+-/1+ ' "
De'<"%3"8 +-+ *'%+' %'&-+ + *+-&*/* 2+1 /%+&-+ *&%&/1+-+ 1' 0'0-&2' (7'/) 5 %'' %'+%4+%&/ + 1'7*/*/ 2&&4&* 1' +2&+-', %'1'* 5 /%&2+; + +1 /%+&-+ *'%+/2' + +-/ +-//.
Ne4t$%&?%)#8 +1 '3?+-&8' *&%&/1 + 1/ *+24%%&6 2+ %&2' 7*/' 5 +1 %'1'*. U '3?+-&8' '2+*' + 1/ *+24%%&6 2+1 %'-+&2' 2+ 0'0-&2' ' 060'*' ()
L%!%3" )"# %<4%8 E1 '3?+-&8' 2+1 1/8/2' %' /74/ + +1&&/* %4/1=4&+* 8+-&7&' 2+ ?/3'+ 5 0'0-&2' *+&24/1+ =4+ +*/+%+ + +1 /%+&-+ 2+4 2+1 %+-*&047/2' *&/*&'.
De"3"$?%)#8 +-+ *'%+' + 11+8/ / %/3' 4-&1&;/2' /1-/ -++*/-4*/ + +1 %4/1 + *+24%&* 1/ %/-&2/2+ 2+ %&2' 1&3*+, + *+-&*/*/ 1' %'4+-' %/4/-+ 2+1 /3'* 5 '1'* -/1+ %'' /12+<@2', /1%'<'1+, &7+-' %'1'*/-+ 5 + -+2* 1/ 2+-*4%%&6 2+ 1' +*6>&2'. S+ *+/1&;/*/ 4-&1&;/2' 1/&/ 2+ +-/1 =4+ +* '+-&2/ / /1-/ -++*/-4*/ %' +1 '3?+-&8' 2+ *'8'%/* 1/ +1&&/%&6 2+ 4-/%&/ 8'1-&1+.
@#te$?%)#: S+ /1&%/*/ 3/?/ -++*/-4*/ /1 /%+&-+ /*/ +/*/* 1' 71&%*&2' /-4*/2' =4+ *+%&&-+ %' +1 %/3&' 2+ -++*/-4*/.
22
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23
-.:.2 ,$4eb%' Q4*)%'
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-.7 DETERMINACION DE ,ROTEINAS
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24
FUNDAMENTO DEL METODO DE MICRO JELDAL.
. D+-*4%%&6 2+ 1/ /-+*&/ '*7&%/ '* /%%&6 2+1 %&2' 410*&%' %'%+-*/2' 5 %/1&+-+. E-+ /%-/ '3*+ 1/ /-+*&/ '*7&%/ 2+<&2*/-2'1/ 5 %/*3'&;2'1/. E1 %/*36 + '>&2/2' 5 +1 &-*67+' *+24%&2' / /'&/%' + *++%&/ 2+ *+/%-&8' ++%@0&%' %'' %/-/1&;/2'*+ +1 /'&/%' 2+*+2&2' =4+2/ 0&?/2' + +1 %&2' 410*&%' %'' 410/-' 2+ /'&'.
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CA,ITULO IV DISEO METODOLÓGICO
:.0 DISEO METODOLOGICO
L/ +-'2'1'7@/ + 2&8&2+ + -*+ /*-+:
:.1 INVESTIGACION BIBLIOGRAFICA E +-/ +-// %'*+2+ + *+/1&;6 1/ &8+-&7/%&6 -+6*&%/ 2+ 1/ ++%&+ + +-42&', 7++*/1&2/2+ 2+ +>-*/%%&6 5 *+0&/%&6 2+ /%+&-+, %''&%&6 =4@&%/, 4', *4+3/ 0&&%'=4@&%/.
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:.-.: ANALISIS FISICOQUIMICO DEL ACEITE :.-.:.1 ,$4eb%' F*')%' C"&"$ P*'%+2&&+-'
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:.-.7 CUANTIFICACION DE ,ROTEINAS EN LA SEMILLA DE Passiflora edulis flavicarpa
MARACUYA/.
METODO DE MICRO9 JELDAL P*'%+2&&+-':
A. DIGESTION:
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B. DESTILACION:
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CA,ITULO V RESULTADOS
7.0 RESULTADOS C4%3$"18 RENDIMIENTO ,ORCENTUAL ANTES DE LA REFINACION
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C4%3$" 28 REFINACION QUIMICA Y FISICA
REFINACION QUIMICA
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PROCESO
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SOLVENTE
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CA,ITULO VI DISCUSIÓN DE RESULTADOS
=.0 DISCUSION DE RESULTADOS Re#3e#t" ("$)e#t4%& 3e& %)ete 3e Passiflora edulis flavicarpa M%$%)456/. D+ "" 0*4-' 2+ Passiflora edulis flavicarpa (M/*/%45) + '3-48' !H. 7 2+ +&11/, +>-*/5+2' ". 7 2+ /%+&-+ =4+ +=4&8/1+ / .H , 1' =4+ 2+4+-*/ =4+ +1 '*%+-/?+ '3-+&2' + *+1/-&8/+-+ 3/?' %' *++%-' / 1/ %/-&2/2 2+ 0*4-' 4-&1&;/2'. D+4 2+ 1/ *+0&/%&6 +1 *+2&&+-' 2&&45+ + 4 . +-' + 2+3+ / =4+ +
-+ %'1+?' *'%+' 04+*' +1&&/2/ 4-/%&/ 8'1-&1+ + &4*+;/
*++-+ + +1 /%+&-+ %*42'. (V+* %4/2*' 2+ *+41-/2' N' ).
Re+#%)# Q4*)% 5 F*')% M+2&/-+ 1' *'%+' 7++*/1+ 2+ 4*&0&%/%&6 /1&%/2' / 1' /%+&-+ 8+7+-/1+ + +1&&/ -'2/ /=4+11/ %/*/%-+*@-&%/ =4+ /0+%-/ 4 %/1&2/2, '* 1' -/-' /1 *+/1&;/*1+ 2&%<' *'%+' + '3-&++: E +1 De'<"%3" , 1/ /2&%&6 2+ 4 /7+-+ =4+1/-+ =4+ + +-+ %/' + +1 %&2' 0'06*&%' + %'3&/%&6 %' +1 /74/, +*&-+ 1/ +1&&/%&6 2+ 7'/ 5 060'*' *++-+ + +1 /%+&-+ 5/ =4+ +-/ <&2*/-/%&6
E 1/ @#te$?%)# 1/ '3-+%&6 2+ 4 /%+&-+ 3*&11/-+ 5 -*//*+-+ + 2+3+ / 1/ +/*/%&6 2+ 1' 71&%*&2' /-4*/2' 1' %4/1+ *+%&&-/ + +1 /%+&-+ 2+3&2' / 1/ /1&%/%&6 2+ 3/?/ -++*/-4*/.
Dete$#%)# 3e ( L/ 2+-+*&/%&6 2+ + 4 /%+&-+ 8+7+-/1 + &'*-/-+ 2+3&2' / 4 *+1/%&6 %' 1/ %/-&2/2 2+ %&2' 7*/' 2+-+*&/2' + +1 @2&%+ 2+ /%&2+;, /2+ 4+2+ -'/*+ %'' 4/ *4+3/ 0@&%/ =4+ 2+-+*&+ 1/ 2+%''&%&6 2+ 4 /%+&-+. E1 /%+&-+ 2+ Passiflora edulis flavicarpa (M/*/%45) *++-/ 4 %&2' 2+ #." (V+* %4/2*' 2+ *+41-/2' N' ) &2&%/2' +1 7*/ %'-+&2' 2+ %&2' 7*/' + 4 %''&%&6.
Dete$#%)# 3e ,4#t" 3e 4" E-+ + 4 %*&-+*&'
2+ &'*-/%&/ /*/ /%+&-+ 5 7*//, 5 2++2+
2+ 1/
%''&%&6 2+ 1' %&2' 7*/' 2+ 1/ 4+-*/. L' /%+&-+ =4+ %'-&++ %&2' 7*/' 2+ 3/?' +' '1+%41/* -&++ 4 4-' 2+ <4' +'*. E1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) *++-/ 4 4-' 2+ <4' /5'* =4+ 1' '-*' /%+&-+ 8+7+-/1+ (/+>' -/31/ !), 1' =4+ &2&%/ =4+ -+ '++ %&2' 7*/' 2+ /1-' +' '1+%41/* +*&-&2'1+ -++* /5'* *+&-+%&/ / /1-/ -++*/-4*/. (V+* %4/2*' 2+ *+41-/2' NK #).
Dete$#%)# 3e V')"'3%3 E1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) / -++*/-4*/
/3&+-+
*++-/ 4/ 8&%'&2/2 3/?/ 2+ #. %. (V+* %4/2*' 2+ *+41-/2' N' ) P'* 1' =4+ + %'&2+*/ %'' 4 /%+&-+ %' 7*/ %//%&2/2 2+ 014&2+; 5 2+ +-' 2++2+* 1/ /1&%/%&6 =4+ -+2*.
Dete$#%)# 3e De#'3%3 L/ 2+&2/2 =4+ *++-/ +1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) / !C + 2+ ".# 7 L (V+* %4/2*' 2+ *+41-/2' N' ) '* 1' %4/1 + 4+2+ '3+*8/* =4+ -+ 8/1'* +- 2+-*' 2+1 */7' =4+ *++-/ 1' /%+&-+ 8+7+-/1+ %' 1' =4+ + ' ! -/31/ ).
Dete$#%)# 3e Ce#?%' L/ 2+-+*&/%&6 2+ %+&;/ + &'*-/-+
/*/ &2&%/* 1/ %/-&2/2 2+ /-+*&/1
61&2' =4+ *++-/ 4 /%+&-+ 2+4 2+ 4 =4+/, L' /%+&-+ 4*' 5 7*// 7++*/1+-+ %'-&++ '%/ %/-&2/2 ' /2/ 2+ %+&;/. T'/2' + %4+-/ =4+ 1/ 7*//, /%+&-+ 5 /-+=4&11/ 8/*@/ 2+ ","" / #," P'2+' 2+%&* =4+ +1 8/1'* *++-/2' '* +1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45): ".# (V+* %4/2*' 2+ *+41-/2' N' ) +-/ 2+-*' 2+1 */7' +-/31+%&2', 2+-+*&2'+ =4+ +1 '*%+-/?+ 2+ /-+*&/1 8'1/-&1&;/2' + 3/?'.
Dete$#%)# 3e #3)e 3e Re+$%))0# L/ 2+-+*&/%&6 2+1 @2&%+ 2+ *+0*/%%&6 + 4/2/ %'' 4/ +2&2/ 2+ 4*+;/ 5 +2&' 2+ &2+-&0&%/%&6. A1 %'/*/* +1 8/1'* '3-+&2' + +1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45): .#! %' 1' '-*' /%+&-+ 8+7+-/1+ =4+ *++-/
8/1'*+ +-*+ .# .#" (/+>' ! -/31/ ) + 4+2+ '3+*8/* =4+ + &&1/* /1 2+ -', '* 1' %4/1 4+2+ 2+-+*&/*+ =4+ +- 2+-*' 2+ 4 */7' 2+ 4*+;/ /%+-/31+. (V+* %4/2*' 2+ *+41-/2' N' H).
Dete$#%)# 3e S"&4b&3%3 L/ '143&1&2/2 2+1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) + *+/1&;6 %' 2&0+*+-+ '18+-+ + 3/+ / 1/ -/31/ 2+ '143&1&2/2+ ++%&0&%/2/ + 1/ USP !# (/+>' ! -/31/ ), *++-/2' /5'* '143&1&2/2 + B+%+' 5 +>/' &+2'
-/ /5'* + +>/', 2+3&2' / =4+ + %//; 2+ 2&'18+* 7*/2+ %/-&2/2+ 2+ 7*// & /**/-*/* '-*' %''+-+ *++-+ + +1 /%+&-+, *++-/2' -/3& +'* -'>&%&2/2 =4+ +1 B+%+'. (V+* %4/2*' 2+ *+41-/2' N' ).
Dete$#%)# 3e& I#3)e 3e A)3e? Q4@&%/+-+ 1' /%+&-+ +- %'4+-' '* %&2' 7*/' ''&/-4*/2', '1&&/-4*/2' 5 /-4*/2', %'&2+*/2' %'' 4 /%+&-+ ''&/-4*/2' /=4+1 =4+ *++-/ + /5'* *''*%&6 +1 %&2' '1+&%' 5 '1&&/-4*/2' +1 =4+ -&++ /5'* *''*%&6 2+ %&2' 1&'1+&%' 5 + %&+*-' /%+&-+ 1&'1&%'#. C'/*/2' %' 1' 2+ /%+&-+ 8+7+-/1+ (/+>' ! -/31/ !) + 4+2+ '3+*8/* =4+ +1 /%+&-+ 2+ passiflora edulis flavicarpa (/*/%45) *++-/ +'* %/-&2/2 2+ %&2' '1+&%' (!.) '* 1' =4+ + 4+2+ %'&2+*/* %'' 4 /%+&-+ '1&&/-4*/2'. (V+* %4/2*' 2+ *+41-/2' N'").
Dete$#%)# 3e I#3)e 3e Y"3" E1 8/1'* 2+ @2&%+ 2+ 5'2' =4+ *++-/ +1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) + 2+ H.", 4+*&'* / 1' /%+&-+ %' 1' =4+ + ' ! -/31/ !),
'* 1' %4/1 +-+ /%+&-+ +
+&31+ / 1/ *+/%%&'+ 2+ '>&2/%&6 2+3&2' / 1/ 7*/ %/-&2/2 2+ &/-4*/%&'+ =4+ '++. (V+* %4/2*' 2+ *+41-/2' N' ).
Dete$#%)# 3e I#3)e 3e ,e$03" E1 8/1'* 2+ +*6>&2' &2+ +1 7*/2' 2+ '>&2/%&6 2+ 1@&2' + 7*// 5 /%+&-+, +*' ' 4 +-/3&1&2/2. E>&-+ *+1/%&6 +-*+ +1 @2&%+ 2+ +*6>&2' 5 1/ */%&2+; 2+ 1/ 4-/%&/ 7*// 5/ =4+ /1 '3-++*+ 4 @2&%+ 2+ 5'2' /1-' -/3& + '3-&++ 4 @2&%+ 2+ +*6>&2' /1-' (/+>' ! -/31/ !), '* 1' %4/1 +1 /%+&-+ 2+ Passiflora edulis
flavicarpa ( /*/%45) -&+2+ *&2/+-+ / +*/%&/*+ '* 4 /1-' @2&%+ 2+
+*6>&2' #.# +=. (V+* %4/2*' 2+ *+41-/2' N' !). /1-+*2'+ '* %'&74&+-+ 4 %/*/%-+*@-&%/ '*7/'1-&%/.
Dete$#%)# 3e #3)e 3e S%("#+)%)0# E1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) '++ 4 /1-' @2&%+ 2+ /'&0&%/%&6: !. (V+* %4/2*' 2+ *+41-/2' N' ) %' *++%-' / 1' /%+&-+ 8+7+-/1+ %' 1' %4/1+ +
H H
(A+>' ! -/31/ !), 1' %4/1 4+2+ -'/*+ %'' /*+-*' /*/ 1/ +1/3'*/%&6 2+ ?/3'+, %'*'3/2' =4+ +1 *'24%-' '3-+&2' + 4 ?/36 4/8+ 5 45 <4+%-/-+.
Dete$#%)# 3e ,$"te*#%' L/ /5'*@/ 2+ +&11/ '1+/7&'/ + %41-&8/ *&%&/1+-+ %'' 04+-+ 2+ /%+&-+. E1 *+&24' =4+ + +>-*/+ 2+ 1/ +1/3'*/%&6 2+ /%+&-+ + 1/ -'*-/ =4+ + 4+1+ /*/ *'24%&* ' -/31/ ) =4+ + 4/ 2+ 1/ / 4-&1&;/2/ + 1/ /1&+-/%&6 /&/1 '* 1' %4/1 4+2+ +* +1+/2/ %'' %'%+-*/2' ' %'1++-' *'-+&%' + %'3&/%&6 %' '-*/
CA,ITULO VII CONCLUSIONES
56
.0 CONCLUSIONES
. C' +1 *'%+' 2+ *+0&/%&6 2+1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45), + '3-&++ %'' *+41-/2' 1/ *+0&/%&6 2+1 /%+&-+ 5 4 ?/36 %'' 43*'24%-' 2+ -+, &+2' /3' 4-&1&;/2' + 1/ &24-*&/ %'-&%/.
!. D+3&2' /1 /1-' @2&%+ 2+ 5'2' 5 P+*6>&2' =4+ *++-/ +1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) + 4+2+ %'%14&* =4+ -+ + 4 /%+&-+ 2+ 0%&1
'>&2/%&6 5 %' /1-' 7*/2' 2+ */%&2+;, '* 1' %4/1 2+3+ *++*8/*+ %' /-&'>&2/-+ /*-&0&%&/1+ /*/ %'+*8/* 4 *'&+2/2+ '*7/'1-&%/.
. E1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) *+'*-/ 4 8/1'* /1-' 2+ @2&%+ 2+ /'&0&%/%&6, '* 1' =4+ + %'%145+ =4+ + 4/ /-+*&/ *&/ 4-&1&;/31+ /*/ 1/ +1/3'*/%&6 2+ ?/3'+.
#. D+ /%4+*2' /1 '*%+-/?+ 2+ *'-+@/ %'-+&2' + 1/ +&11/ 2+ Passiflora edulis flavicarpa (/*/%45) '2+' 2+-+*&/* +1 4' 2+ -/ %'' 4/ /1-+*/-&8/ 2+
04+-+ 2+ *'-+@/ + 1/ /1&+-/%&6 /&/1, *+/1&;/2' *+8&/+-+ 4 +-42&' -'>&%'167&%'.
57
. L/ %/*/%-+*&;/%&6 2+1 /%+&-+ 2+ P/&01'*/ +241& 01/8&%/*/ (/*/%45) + &'*-/-+ 5/ =4+ / -*/8 2+ 1/ *4+3/ 0&&%'=4@&%/ *+/1&;/2/ + 4+2+ %''%+* 1/ *'&+2/2+ =4+ *++-/, +-/31+%&+2' %' +11' 4/ 3/+ -+6*&%/ /*/ +-42&' '-+*&'*+.
. P'* 1' *+41-/2' '3-+&2' + 1/ 2+-+*&/%&6 2+1 4-' 2+ <4' + %'%145+ =4+ +1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) *++-/ *+&-+%&/ / /1-/ -++*/-4*/.
CA,ITULO VIII RECOMENDACIONES
59
H.0 RECOMENDACIONES
. E *+%'+2/31+ &%+-&8/* +1 %41-&8' 2+ 1/ ++%&+ /*/ '2+* %'-/* %' 4/ %/-&2/2 2+ /-+*&/ *&/ 40&%&+-+ 5 '2+* 11+8/* / %/3' 4 *'%+' &24-*&/1 / 3/?' %'-'.
!. S+ *+%'&+2/ %'-*'1/* *&74*'/+-+ 1/ %'2&%&'+ 2+ -*/3/?', 8/*&/31+ 2+ %'-*'1 5 -&' 2+ '+*/%&6 (8+* /+>' ) 24*/-+ %/2/ /' 2+1 *'%+' 2+ *+0&/%&6 =4@&%/ 5 0@&%/, 1' %4/1+ ' 2+ 7*/ &'*-/%&/ /*/ '3-++* 4 *'24%-' 2+ %/1&2/2 /%+-/31+.
. S+ *+%'&+2/ +1 4' 2+1 /%+&-+ 2+ Passiflora edulis flavicarpa (/*/%45) %'' /-+*&/ *&/ /*/ 1/ +1/3'*/%&6 2+ ?/3'+ + 1/ &24-*&/ %'-&%/, 2+3&2' /1 /1-' @2&%+ 2+ /'&0&%/%&6 =4+ *++-/.
#. R+/1&;/* 4 +-42&' -'>&%'167&%' /1 *+&24' 2+ 1/ +&11/ 2+ Passiflora edulis flavicarpa (M/*/%45) /*/ 4 /1&%/%&6 + 1/ *+/*/%&6 2+ %'%+-*/2'
4-&1&;/2' + 1/ /1&+-/%&6 /&/1, 5/ =4+ *++-/ 4 /1-' '*%+-/?+ 2+ *'-+@/.
. S+ *+%'&+2/ *+/1&;/* '-*' +-42&' /1 /%+&-+ 2+ Passiflora edulis flavicarpa (M/*/%45) /*/ 2+-+*&/* 4 4' %'' /%+&-+ 143*&%/-+, 2+3&2' / =4+ +-+ *++-/ *+&-+%&/ / /1-/ -++*/-4*/.
BIBLIOGRAFA
BIBLIOGRAFIA .
A**&'1/ G'+;,G. “C'*'3/%&6 2+ 4*+;/ 2+ /%+&-+ %'+-&31+ 2+ 2&0+*+-+ /*%/ %'+*%&/1+ + +1 *+/ +-*''1&-//. F/%41-/2 2+ Q4@&%/ 5 F/*/%&/. U&8+*&2/2 2+ E1 S/18/2'*.!"".
!.
F/*/%'+/ 2+ 1' E-/2' U&2' M+>&%/', +2&%&6, -'' I 5 II, M>&%', !""".
.
M/-/ C/-/+2/, C.A.
“C4/-&0&%/%&6 2+1 %'-+&2' 2+ *'-+@/ -'-/1 + +1
3/7/;' 2+ 1/ /*/?/ 8/1+%&/ (C&-*4 @+&) /1 +* +*&=4+%&2/ %' +1 A+*7&114 N@7+*. F/%41-/2 2+ Q4@&%/ 5 F/*/%&/. U&8+*&2/2 2+ E1 S/18/2'*.!"". #.
P
.
P''1, A. “F1'*/ 2+ N&%/*/74/. A7&'+*/”. T'' II 8'14+ H, +2&-'*&/1 M&'4*& B'-/&%/1 G/*2+, N&%/*/74/ !"".
.
R+5/ 2+ A74&1/*, M.L. “L/ P/&01'*/”. B'1+-@ I0'*/-&8' 2+1 J/*2@ B'-&%' L/ L/74/, .
.
R'2*@74+;, B.M. “A1&& 2+ A1&+-'”, -'' I, 4&8+*&2/2 %+-*/1 2+ V++;4+1/, '*7/&;/%&6 2+ 3&++-/* +-42&/-&1, V++;4+1/ H".
H.
_&-', C.J.” A%+&-+ V+7+-/1+``, -'' 8+&-+ 5 2', +2&-'*&/1 P//+*&%//, B4+' A&*+, A*7+-&/.#.
.
./7.4&4%.+24a//1/+/'1*'0&1+NOTD+%H.<- C'41-/2' +1 2+ /7'-' !"".
".
./13/+>.%'FORRAMEL*'%+''5.<- C'41-/2' +1 2+ A7'-' !"".
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
ANEXOS
ANEXO
F&74*/ NK M/*/%45 /-+ 2+ +* 1/-/2/
F&74*/ NK ! P1/-/%&6 2+ M/*/%45
F&74*/ NK P1/-/ 2+ M/*/%45
F&74*/ NK # F*4-'
F&74*/ NK S+&11/ 2+1 0*4-'
F&74*/ N E=4&' 2+ +>-*/%%&6 S'><1+-
ANEXO 2 TABLA NK : SOLUBILIDAD (USP !#)
OBSERVACIONES
,ARTES DE SOLVENTE ,OR UNA ,ARTE DE SOLUTO
M45 '1431+
M+' 2+
L&3*++-+ '1431+
D+ "
S'1431+
D+ " "
P'%' '1431+
D+ " ""
L&7+*/+-+ '1431+
D+ "" """
M45 1&7+*/+-+ '1431+
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T/31/ !: PRUEBAS QUIMICAS DE ACEITES VEGETALES
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ANEXO -
ANEXO : PROCESO INDUSTRIAL DE REFINACION QUIMICA Y FISICA REALIZADO EN “EL DORADO”
REFINACION QUIMICA
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EXTRACCION Y CUANTIFICACION DEL ACEITE ,"$)e#t%je 3e %)ete "bte#3" %#te' 3e &% $e+#%)0#: E>-*/%%&6 b : C/-&2/2 2+ +&11/: H.H 7*/' C/-&2/2 2+ /%+&-+ '3-+&2' /-+ 2+ 1/ *+0&/%&6: .! 7*/'
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