NUMERACION DE STAPHYLOCOCUS AUREUS COAGULASA POSITIVO

PRACTICA N°4: NUMERACIÓN DE STEPHYLOCOCUS AUREUS COAGULASA POSITIVO

NUMERACION DE STAPHYLOCOCUS AUREUS COAGULASA POSITIVO I.

INTRODUCCIÓN Staphylococc! a"#! #! $a #!p#c%# &act#"%a$a %$t#'"a(a po" )o"*a! coc+c#a! (# ,-./0-, ** (# (%+*#t"o- 1# !# (%2%(#$ #$ *+! *+! (# $ pla$ pla$oo- po" po" lo 1# 1# !# a'" a'"pa pa$ $ %"" %""#'l #'la" a"*# *#$t $t# # #$ "ac%*o!3 So$ %$*2%l#! y $o )o"*a$ #!po"a!3 So$ '"a* po!%t%2a!3 E! $a $a #!p# #!p#c% c%# # *y *y !#$ !#$!%&l !%&l# # a la acc% acc% $ (#l (#l calo" alo" y (# lo! lo! (#!%$)#cta$t#!3 M+! (#l 5, 6 (# la! p#"!o$a! !o$ po"ta(o"a! (# S3a"#! #$ )o!a! $a!al#!$a!al#!- 'a"'a$ta'a"'a$ta- )o"7$clo!)o"7$clo!- *a$o! o p#lo3 p#lo3 Po" #!o ! p"#!#$c%a #$ (#t#"*%$a(o! $%2#l#! #$ lo! al%*#$to! #! $ !%'$o #2%(#$t# (# )alta (# h%'%#$# #$ la *a$%plac%$3 A(#*+! al'$a! c#pa! (# S3a"#! !o$ pat'#$a! pa"a #l ho*&"# po"1# p"o(c#$ #$t#"oto8%$a! 1# (a$ l'a" a la %$to8%cac%$ #!ta9locc%ca3 La (o!%! %$)#ct%2a #! alta ya 1# #! $#c#!a"%o $ $7*#"o (# al *#$o! 0,  )c;' pa"a p"o(c%" ca$t%(a( !9c%#$t# (# #$t#"oto8%$a pa"a p"o(c%" la #$)#"*#(a( #$ #l co$!*%(o"3 El 1#! 1#!o o )"#!c )"#!co o a"t# a"t#!a !a$al $al #! $ (#"% (#"%2a( 2a(o o l+ct l+ct#o #o a*pl a*pl%a %a*#$ *#$t# t# co$!*%(o co$!*%(o #$ P#"7 P#"7 y !# #8p#$(# #8p#$(# #$ $a ca$t%(a( ca$t%(a( ap"#c%a&l ap"#c%a&l# # #$ lo! lo! *#"ca #"ca(o (o!! *$% *$%c% c%pa pal# l#!!- (o$( (o$(# # #l p7&l p7&l%%co co$! o$!*% *%(o (o"" '#$#"al*#$t# $o co$oc# la p"oc#(#$c%a $% la )o"*a (# #la&o"ac%$- la cal !# "#al% #>o o y 1#1#- !%$ !%$ #*&a #*&a""'o'o- #8%! #8%!ta ta ca$t%(a( (#t#cta&l# !9c%#$t# (# #$t#"oto8%$a #!ta9locc%ca3 E$ #!t# ca!o- lo! *%c"oo"'a$%!*o! 1# o"%'%$a"o$ la to8%$a ha$ %(o (#!c#$(%#$(o #$ $7*#"o #- %$cl!o- (#!apa"#c%#$(o- *%#$t"a! 1# la to8%$a- po" ! *ayo" "#!%!t#$c%a- p#"*a$#c# #$ #l al%*#$to3

ING. AMELIA CASTRO GAMERO

P+'%$a 1


-2014

El *=to(o (# "#c#$to #$ placa !# t%l%
II. FUNDAMENTO TE TEORICO a) GENERA GEN ERALID LIDADE ADES S

Lo! #!ta9lococo! !o$ coco! a$a#"o&%o! )acltat%2o!- !o$ G"a* po!%t%2o! y !# p"#!#$ta$ !olo!- #$ pa"#! o "ac%*o!- $o !o$ *2%l#!- $% #!po"la(o!? al'$o! &%ot%po! !o$ capac#! (# p"o(c%" $a to8%$a alta*#$t# t#"*o#!ta&l#a!@ po " ##*ploStaphylococc! a"#! p"o(c# 5 to8%$a!1# p#(#$ p"o2oca" !#2#"a! %$to8%cac%o$#! #$ #l ho*&"#3 S *#ta&o *#ta&ol%! l%!*o *o #! o8%(at% 8%(at%2o;) 2o;)#"* #"*#$t #$tat% at%2o2o- #! catala catala!a/p !a/po!% o!%t%2 t%2o o y p#(# p#(# *#ta&ol%a! ca$t%(a(#! (# &%8%(o (# ca"&o$o? #$ co$(%c%o$#! a$a#"o&%a!- #l p"o(cto p"%$c%pal (# la )#"*#$tac%$ #! #l +c%(o l+ct%co3 La! t"#! co$(%c%o$#! $#c#!a"%a! pa"a ! pt%*o (#!a""ollo !o$: pH c#"ca$o a la $#t"al%(a(- t#*p#"at"a al"#(#(o" (# B,C y a!#$c%a (# *%c"oo"'a$%!*o! co*p#t%t%2o!3 E!t# 7lt%*o p$to #! %*po"ta$t#- ya 1# Staph aphylococc! a" a"#! $o #! co*p#t% #t%t%2o #$ p"#!# #!#$c%a (# ot"o! *%c"oo"'a$%!*o!3


P+'%$a 2


-2014

STAPHYLOCOCCUS SE PUEDE ENCONTRAR EN( aD medio amie!"e #omo $%ede &e'(

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 p o " # A# * p l o lo ! 1  #  E ! t o ! 7 l t%* o ! p " # ! # $ t a $  $ a l to c o $ t # $ % ( o p " o t # %c o - c o * o p  # ( # ! # " l a lo ! a $ % * a l # ! y l#ch# y (#"%2a(o! l+ct#o!la ! p # " ! o $ a ! ! o $ t a * & % = $ ! # ( # ! a " " o ll a # $ a 1  # l l o ! a l %* # $ t o ! 1  # lo! p"%$c%pal#! p"#!#$ta$ alta! "#!#"2o"%o! (# co$c#$t"ac%o$#! (# !al- $o (# #llo! !#"@a #l Aa* 4$? ot"o #!to! )acto" %*po"ta$t# #$ lo! a l %* # $ t o ! # ! # l p H - a ! @ *%c"oo"'a$%!*o! t#$#*o ! #$ #l ca!o (# la 3 * a y o $ # ! a - = ! t a t% # $ #  $ p H lo !9c%#$t#*#$t# &aAo pa"a %$h%&%" #l (#!a""ollo (# S 3 a  " #  ! - ! %$ # * & a " ' o - a l (%l%"!# y $#t"al%
 a%"#- pol2o!p#"9c%#! #$ (o$(# !# *a$#Aa$ a l %* # $ t o ! - a '  a a '  a " # ! %(  a l -

*a$#o o &%#$ a t#*p#"at"a! (# co$!#"2ac%$ %$ap"op%a(a!? #!t# p"o&l#*a !# ac#$t7a po" la a!#$c%a (# Fo"a co*p#t%t%2a- 1# $o"*al*#$t# "#!t"%$'# #l (#!a""ollo (# #!t# *%c"oo"'a$%!*o3

El c"#c%*%#$to (# Staphylococc! a"#! #$ al%*#$to!- t%#$# '"a$ %*po"ta$c%a po" t"ata"!# (# $ *%c"oo"'a$%!*o capa< (# p"o(c%" $a po(#"o!a #$t#"oto8%$a- 1# al %$'#"%"!# ca!a %$to8%cac%o$#! !#2#"a! al ho*&"#3 Co*o !# *#$c%$ o co$ a$t#"%o"%(a( !o$ 5 #$t#"oto8%$a!: A- - C- D y E- !%#$(o la A la *+! $oc%2a3 ING. AMELIA CASTRO GAMERO

P+'%$a -


-2014

La ca$t%(a( (# Staphylococc! a"#! $#c#!a"%a pa"a p"o(c%" !9c%#$t# to8%$a J03,*%c"o'"a*o- pa"a ca!a" %$to8%cac%$- #! (# 0,UKC;' (# al%*#$to- !%$ #*&a"'o a&lo$!% #t al- co*#$ta$ 1# #l KDA- #!ta&l#c# 1# $a ca$t%(a( (# Staphylococc! a"#! pat'#$o- #$ #l 1# !# #$c#$t"#$ 0,5UKC;' (# al%*#$to- p"o2oca %$to8%cac%o$#! y 1# $ $%2#l &a!al (# ap"o8%*a(a*#$t# $ $a$o'"a*o (# to8%$a #!ta9loccc%ca po" '"a*o (# al%*#$to- #! !9c%#$t# pa"a ca!a" !@$to*a! a!oc%a(o! co$ la %$to8%cac%$ a$t#! *#$c%o$a(a3 Ta*&%=$ #! %*po"ta$t# o&!#"2a"- 1# #$ la *ayo"@a (# lo! &"ot#! (# %$to8%cac%$ ca!a(o! po" #!t# *%c"oo"'a$%!*o- !# ha$ (#t#cta(o $%2#l#! (# $o a c%$co *%c"o'"a*o! (# to8%$a %$'#"%(a- a$1# #$ al'$o! ca!o! la! co$c#$t"ac%o$#! (# to8%$a ha$ !%(o a7$ *+! &aa!- (#l o"(#$ (# ,3,0 *%c"o'"a*o- !9c%#$t#! pa"a p"o2oca" $a %$to8%cac%$ #$ #l ho*&"#3 Lo! al%*#$to! co$ta*%$a(o! co$ #!ta ca$t%(a( (# &act#"%a!- $o p"#!#$ta$ $%$'$a (%)#"#$c%a p#"c#pt%&l# #$ ca$to a ! apa"%#$c%a- !a&o" y olo"- po" lo 1# $o !# (%!t%$'#$ (# lo! al%*#$to! 1# $o #!t+$ co$ta*%$a(o! co$ #!t# *%c"oo"'a$%!*o- po" #!ta "a<$ #l p#l%'"o (# %$'#"%" al%*#$to! co$ta*%$a(o! co$ Staphylococc! a"#! !%$ (a"!# c#$ta #! alto3 Lo! al%*#$to! !o*#t%(o! a %$t#$!a *a$%plac%$ ("a$t# ! p"#pa"ac%$ y 1# !# *a$t%#$#$ a t#*p#"at"a! (# "%#!'o Jpo" #$c%*a (# 3° C y po" (#&ao (# ,° C (#!p=! (# ! p"#pa"ac%$- !o$ lo! al%*#$to! *+! %$2olc"a(o! #$ la %$to8%cac%$ #!ta9loccc%ca3 Lo! aime!"o& $e'e#ede'o& tal#! co*o ca"$#! c"(a! y p"oc#!a(a!#$!ala(a!- p"o(cto! (# pa!t#l#"@a- co*o pa!t#l#! "#ll#$o! co$ c"#*a- p%#! (# c"#*a- co&#"t"a! (# chocolat#- l#ch# y !! (#"%2a(o!- "#ll#$o! pa"a !+$(%ch#! y papa!- !o$ lo! *+! co*7$*#$t# a!oc%a(o! co$ %$to8%cac%o$#! #!ta9loccc%ca!3 E! o& %ma!o&/ la! c#pa! (# Staphylococc! ha&%ta$ #$ )o"*a #!p#c@9ca #$ #l t"acto $a!o)a"@$'#o- ca&#llo y p%#l (#l 5,6 o *+! (# lo! %$(%2%(o!- !%$ ca!a" (a>o apa"#$t# Jpo"ta(o"#! a!%$to*+t%co!- p(%#$(o t"a$!)#"%"!# (# lo! (#(o! y *a$o! a lo! al%*#$to! y (#!a""olla"!# co$ "ap%(#< #$ #llo!3


P+'%$a 0

PRACTICA N°4: NUMERACIÓN DE STEPHYLOCOCUS AUREUS COAGULASA POSITIVO La #!p#c%# a"#!- #! la co$!%(#"a(a pat'#$a (#l '=$#"o Staphylococc! y #!t+ co*7$*#$t# a ca!o! (# a"t"%t%!- o!t#o*%#l%t%!- *#$%$'%t%!$#*o$@a y #$ al'$o! ca!o! p#(# ll#'a" a oca!%o$a" p="(%(a (#l co$oc%*%#$to3 La! c#pa! *%c"oo"'a$%!*o 1# p"o(c#$ #$<%*a! #8t"ac#lla"#! y to8%$a!- !o$ la! *+! %*po"ta$t#! po" !#" ca!a$t# (# %$to8%cac%o$#! al%*#$ta"%a!3

-2014

(#$t"o a!oc%a(o (#

#!t#

El t="*%$o Staphylococc! coa'la!a/po!%t%2o !# t%l%
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Co$9"*a" la p"#!#$c%a (# #!t# *%c"oo"'a$%!*o co*o a'#$t# ca!al (# %$to8%cac%o$#!3 D#t#"*%$a" !% $ al%*#$to o %$'"#(%#$t# (# lo! *%!*o!- #! )#$t# pot#$c%al (# #!t# *%c"oo"'a$%!*o #$t#"oto8%'=$%co3 D#*o!t"a" la co$ta*%$ac%$ po!tp"oc#!o- la cal #! !al*#$t# (#&%(a a co$tacto h*a$o o co$ !p#"9c%#! %$a(#ca(a*#$t# !a$%t%
) Mo'oo3a El S3 a"#! #! $ coco %$*2%l- (# ,3. a 0 *%c"*#t"o (# (%+*#t"o- 1# !# (%2%(# #$ t"#! pla$o! pa"a )o"*a" '"po! (# c=lla! %""#'la"#! !#*#a$t#! a "ac%*o! (# 2a!3 E$ #8t#$(%(o! (# p! lo! coco! apa"#c#$ !olo!- #$ pa"#!- #$ "ac%*o! o #$ ca(#$a! co"ta!3 Lo! "ac%*o! %""#'la"#! !o$ ca"act#"@!t%co! (# #8t#$(%(o! to*a(o! (# clt%2o! 1# !# (#!a""olla$ #$ *#(%o! !l%(o!- *%#$t"a! 1# #$ ot"o! clt%2o! !o$ )"#c#$t#! la! )o"*a! (# (%plococo! y #$ ca(#$a! co"ta!3 U$a! poca! c#pa! p"o(c#$ ING. AMELIA CASTRO GAMERO

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-2014

$a cap!la o capa (# &a&a 1# %$c"#*#$ta la 2%"l#$c%a (#l *%c"oo"'a$%!*o3 El S3 a"#! #! $ *%c"oo"'a$%!*o G"a*po!%t%2o p#"o la! c=lla! 2%#a! y lo! *%c"oo"'a$%!*o! )a'oc%ta(o! !# t%>#$ co*o '"a*$#'at%2o!3

#) FUNDAMENTO DEL LA PRUE5A La p"#!#$t# t=c$%ca pa"a la (#t#cc%$ (# Staphylococc! a"#!- co$!%!t# #$ lo! !%'%#$t#! pa!o!: 03 A%!la*%#$to !#l#ct%2o- !# t%l%a(a! (# Staphylococc! a"#!3 B3 I(#$t%9cac%$ &%o1@*%ca- #$ #!t# p$to !# %(#$t%9ca #l '=$#"o y #!p#c%# (# Staphylococc! a"#! #$t#"oto8%'=$%co3 E!ta! p"#&a! &%o1@*%ca! J(# ac#"(o la No"*a O9c%al !o$: p"#!#$c%a (# la #$<%*a coa'la!a y p"#!#$c%a (# la #$<%*a t#"*o$cl#a!a3 La ca"act#"%
a

po!%t%2a P+'%$a 6

PRACTICA N°4: NUMERACIÓN DE STEPHYLOCOCUS AUREUS COAGULASA POSITIVO P"#!#$c%a (# t#"*o$cl#a!a

-2014

po!%t%2a

La (#t#"*%$ac%$ (#l Staphylococc! a"#! *#(%a$t# #8t#$!%$ (#l %$clo #$ !p#"9c%#- #! a(#ca(o pa"a #l a$+l%!%! (# al%*#$to! #$ lo! 1# !# #!p#"# #$co$t"a" *+! (# 0,, c=lla! (# Staphylococc! a"#!;'  0, c=lla! (# Staphylococc! a"#!; *L (# al%*#$to3 El a!p#cto ca$t%tat%2o #$ la %$2#!t%'ac%$ (# Staphylococc! a"#! #! (# !*a %*po"ta$c%a- $o !lo (#!(# #l p$to (# 2%!ta #co$*%co- !%$o #$ #l (# !al( p7&l%ca? la No"*a #!ta&l#c# $ l@*%t# *+8%*o (# 0,UKC;' (# al%*#$to pa"a 1# =!t# p#(a !#" co$!*%(o3 D#!(# #!t# p$to (# 2%!ta y (# ac#"(o co$ la t=c$%ca *#$c%o$a(a- la !#$!%&%l%(a( *@$%*a (# (#t#cc%$ #$ #l "#c#$to #$ placa- t%l%
EL 7UESO PARIA E! $ 1#!o !#*%("o 1# !# p"o(c# #$ #l alt%pla$o p#"a$o3 E! (# l#ch# &o2%$a y ! p"o(cc%$ #!t+ *y #8t#$(%(a !o&"#to(o #$ #l $o"t# (# la R#'%$ P$o- (# (o$(# a ! 2#< #! o"%'%$a"%a3 Po!## $a co"t#
7UESO FRESCO


P+'%$a 8


-2014

El 1#!o )"#!co o 1#!o &la$co #! $ t%po (# 1#!o &la$(o- #! (#c%" "#t%#$# '"a$ pa"t# (#l !#"o y $o t%#$# p"oc#!o (# *a("ac%$ o "#9$a(o3 La )a&"%cac%$ (# #!t# 1#!o #! *y !#$c%lla3 El caa(o #! #!#$c%al*#$t# l+ct%co y ("a $o"*al*#$t# 4 ho"a!- a$1# a 2#c#! *+!3 El (#!#"a(o- ca$(o #! #!t%*la(o po" "pt"a (# la caa(a !#'%(a (# p"#!%$- $o #! $$ca #8c#!%2o y a(#*+! lo! 1#!o! )"#!co! !o$ !%#*p"# h7*#(o! J,/.,6 (# a'a- lo 1# ca!a 1# !#a$ *y poco co$!#"2a&l#! y 1# ! t"a$!po"t# #$ la"'a! (%!ta$c%a! !#a *y (%)@c%l3 Po(#*o! (%!t%$'%" lo! 1#!o! )"#!co! !#'7$ ! *o(o (# (#!#"a(o: (#!#"a(o #$ *ol(#! J1#!o! (# p%#- (# "='%*#$- #$ !aco! o #$ t#la! Jp"oc#(%*%#$to #"'#? la pa!ta 1# !# o&t%#$# !# 2#$(# a '"a$#l Jcaa(a *a'"a o '"a!a y ta*&%=$ *ol(#a(a y co$ )o"*a JN#!chQt#l- Go"$ayS%!!#- #tc33

NORMA SANITARIA SO5RE CRITERIOS MICRO5IOLOGICOS DE CALIDAD SANITARIA E INOCUIDAD PARA LOS ALIMENTOS Y 5E5IDAS DE CONSUMO


P+'%$a 9


-2014

HUMANO

III. O5:ETIVO 

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D#t#"*%$a" !% la! *#!t"a! !o$ apta! pa"a co$!*o (# ac#"(o co$ #l $7*#"o (# $%(a(#! )o"*a(o"a! (# colo$%a! po" '"a*o o *%l%l%t"o (# *#!t"a- "#)#"%(o! #$ la &%&l%o'"a)@a3



Co*pa"a" 1# *#!t"a t%#$# *ayo" ca$t%(a( (# *%c"oo"'a$%!*o! !taphylococ! a"#! y a 1# !# (#&# #!o3

IV. MATERIALES Y E7UIPOS


P+'%$a ;

PRACTICA N°4: NUMERACIÓN DE STEPHYLOCOCUS AUREUS COAGULASA POSITIVO           

V.

-2014

Lo! "#1%!%to! $#c#!a"%o! pa"a la p"#pa"ac%$ y (%lc%$ (# la *#!t"a (# al%*#$to!3 M#!t"a! (# 1#!o )"#!co y 1#!o pa"%aa3 Placa! P#t"% (# 2%("%o J0,,805** o (# pl+!t%co (# ,805**3 P%p#ta! (# 0*l3 G"a(a(a! al ,303 E8t#$!o"#! (# 2%("%o3 a>o (# a'a "#'la(o a 44/4C3 I$c&a(o"a "#'la(a a B5/BC3 A'a" a%"(/PARER3 Cal(o (# c#"#&"o co"a<$3 T&o! (# #$!ayo (# 580,*l- co$t#$%(o ,3B*l (# pla!*a (# co$#o3 G"a(%lla!

PROCEDIMIENTO E
3 P%p#t#a" ,30*l (#l ho*o'#$#%

P+'%$a 1=


-2014

B3 I$c&a" la! placa! #$ po!%c%$ %$2#"t%(a a BC ("a$t# B, y 4. ho"a!3

43 D#!p=! (# B, ho"a! (# %$c&ac%$ !#l#cc%o$a" la! placa! 1# p"#!#$ta$ #$t"# , y ,, colo$%a! y co$ta" to(a! a1#lla! colo$%a! $#'"a! y &"%lla$t#! co$ &o"(# &la$co a$'o!to "o(#a(a! (#

P+'%$a 11


-2014

53 Ma"ca" la po!%c%$ (# #!ta! colo$%a! a %$c&a" la! placa! ("a$t# 0. ho"a! a(%c%o$al#!3

3 Al 9$al (#l p#"%o(o (# %$c&ac%$ J4.ho"a! co$ta" to(a! la! colo$%a! co$ la! ca"act#"@!t%ca! (#!c"%ta! a$t#"%o"*#$t# y a(#*+! a1#lla! colo$%a! $#'"a! &"%lla$t#! co$ o !%$ &o"(# &la$co y !%$
8. PRUE5A DE LA COAGULASA ING. AMELIA CASTRO GAMERO

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a) S#*&"a" la! colo$%a! !#l#cc%o$a(a! #$ Cal(o (# c#"#&"o co"a<$ # %$c&a" a B5/B°C3 ("a$t# , a 4 ho"a!3 ) A(%c%o$a" ,30*l (#l clt%2o a t&o! (# 580,** 1# co$t#$'a ,3B*l (# pla!*a (# co$#o %$c&a" a B5°/B°C3 #) E8a*%$a" (#!p=! (# 4 ho"a! (# %$c&ac%$ !% #l pla!*a ha p#"(%(o ! F%(#< o !# #$c#$t"a $ co+'lo *+! o *#$o! '"a$(#3 S% la "#acc%$ #! $#'at%2a- %$c&a" lo! t&o! a t#*p#"at"a (# la&o"ato"%o y "#/#8a*%$a" (#!p=! (# 4 ho"a!3 La! (%)#"#$t#! "#acc%o$#! (# coa'la!a !# o&!#"2a$ #$ la ta&la (# p$tac%$3 9. Calcla" (#l total (# colo$%a! ca"act#"@!t%ca!- la p"opo"c%$ (# la colo$%a! po!%t%2a! a la! p"#&a (# la coa'la!a t#$%#$(o #$ c#$ta la (%lc%$ #*pl#a(a y #8p"#!a" #l $7*#"o (# Staphylococc! coa'la!a po!%t%2o po" '"a*o o *%l%l%t"o (# *#!t"a

VI. RESULTADOS •

L#'o (# #8t"a#" la! placa! (# la %$c&a(o"a al t"a$!c""%" la! 4. ho"a!!# o&!#"2 lo !%'%#$t#:


P+'%$a 1-


-2014

S# #$*#" la ca$t%(a( (# colo$%a! (# !taphylococ! a"#! p"#!#$t#! #$ ca(a placa (# ca(a *#!t"a o&t#$%#$(o la !%'%#$t# ta&la (# "#!lta(o!3

•

Di%#i >! N%me'o de #oo!ia& e!( #!o )"#!co

1=?1

>

#!o pa"%a

>

1=?2

1=?0

1=?4

Re&%"ado

,, 0,,

B

,

,, 4



,

3580,4)c; ' 380,4)c; '

,,

>

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>

1=?-

Coa%a&a $o&i"i@a La 'ea##i>! e& $o&i"i@a #%a!do e #o%o o'mado e& B'me. Coa%a&a !ea"i@a La 'ea##i>! e& !ea"i@a #%a!do !o &e #%a%a e $a&ma.

OBSERVACIONES: Los datos y resultados obtenidos en la presente practica fueron sacadas de páginas eb y to!adas co!o referencia las indicaciones de la profesora en clase" debido a #ue no se pudo reali$ar la practica por proble!as de construcci%n&

VII. DISCUSIÓN DE RESULTADOS 

Co*o !# p#(# 2#" #$ #l ca("o (# "#!lta(o! #l 1#!o )"#!co po!## *ayo" ca$t%(a( (# colo$%a! S3 a"#! a$1# !#a po" $a l%'#"a (%)#"#$c%a (# c%#$to! (# c=lla!3



D# ac#"(o a lo! "#!lta(o! !# p#(# (#c%" 1# la! "a

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-2014

*a$#"a #$ c*o !# 'a"(a #l 1#!o #$ lo! *#"ca(o! (o$(# !# 2#$(#$- ya 1# #!to! 1#!o! )"#!co! !o$ #8p#!to! al *#(%o !%$ $%$'$a p"ot#cc%$ y po" !#" $ l'a" (o$(# c%"cla *cha '#$t# ta*&%=$ p(o co$t"a#" c=lla! (# S3 a"#!3 Y al (#c%" to(o #!to !# (#&# c"##" 1# #l 1#!o pa"%a #!ta&a #$ *#o"#! co$(%c%o$#! (# h%'%#$# al p"#pa"a"!# y 2#$(#"!#3 

VIII.

Co*pa"a$(o lo! "#!lta(o! co$ lo! 2alo"#! p#"*%!%&l#! (# )c;' (# S3 a"#!- (# ac#"(o a la &%&l%o'"a)@a- !# t%#$# 1# la ca$t%(a( (# S3 a"#! #$ la! *#!t"a! (# 1#!o #! *ayo" a la ca$t%(a( p#"*%t%(a pa"a a*&a! *#!t"a!- p#"o a$ a!@ !# p#"*%t# ! co$!*o #$ la! p#"!o$a! p#!to 1# #! *#$o" a 0,)c;' 1# #! la ca$t%(a( $#c#!a"%a pa"a 1# #l co$!*%(o" !# #$)#"*#- a$ a!@ $o !# "#co*%#$(a ! co$!*o3

CONCLUSIONES 

E$ "#!*#$- la p"#!#$c%a (# $ $7*#"o #l#2a(o (# Staphylococc! a"#! #$ $ al%*#$to "#F#a h%'%#$# (#)#cto!a po" *ala *a$%plac%$3 S%- a(#*+!- #l S3 a"#! a%!la(o !o$ c#pa! #$t#"oto8%'=$%ca!- !po$#$ $ "%#!'o pa"a la !al(3



S# o&t2o pa"a #l 1#!o )"#!co 3580,4)c (# S3 a"#!;'3 (# al%*#$to y pa"a #l 1#!o pa"%a 380,4)c;'3 (# al%*#$to3



Co*pa"a$(o a*&a! *#!t"a!- #l 1#!o )"#!co t%#$# *ayo" ca$t%(a( S3 a"#! p"#!#$t# #$ ! co*po!%c%$- lo cal (#&#"@a !#" al co$t"a"%o#!to !# (#&# a la )alta (# h%'%#$# #$ ! #la&o"ac%$ y al #8po$#" #l 1#!o 9$al%


La (#t#"*%$ac%$ (# *%c"oo"'a$%!*o! !3 a"#! #! %*po"ta$t# pa"a (#t#"*%$a" la cal%(a( 1# t%#$#$ lo! al%*#$to!- 1# !o$ (#!t%$a(o! pa"a co$!*o o #la&o"ac%$ (# p"o(cto! (# co$!*o y 2#"%9ca" 1# $o t#$'a$ ot"o t%po (# &act#"%a! o a'#$t#! pat'#$o!3 Pa"a #llo !# t%l%

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y la )o"*ac%$ (# colo$%a! $#'"a! co$ halo t"a$!pa"#$t# #$ *#(%o (# clt%2o A'a" a%"( Pa"#"3

I<. CUESTIONARIO 1. XC>mo &e a$i#a e&"e i!di#ado' mi#'oio>i#o e! a $'od%##i>! de a e#e , $'od%#"o& #"eo&

La (#t#"*%$ac%$ (#l Staphylococc! a"#! *#(%a$t# #8t#$!%$ (#l %$clo #$ !p#"9c%#- #! a(#ca(o pa"a #l a$+l%!%! (# al%*#$to! #$ lo! 1# !# #!p#"# #$co$t"a" *+! (# 0,, c=lla! (# Staphylococc! a"#!;'  0, c=lla! (# Staphylococc! a"#!; *L (# al%*#$to3 El a!p#cto ca$t%tat%2o #$ la %$2#!t%'ac%$ (# Staphylococc! a"#! #! (# !*a %*po"ta$c%a- $o !lo (#!(# #l p$to (# 2%!ta #co$*%co- !%$o #$ #l (# !al( p7&l%ca? la No"*a #!ta&l#c# $ l@*%t# *+8%*o (# 0, UKC;' (# al%*#$to pa"a 1# =!t# p#(a !#" co$!*%(o3 D#!(# #!t# p$to (# 2%!ta y (# ac#"(o co$ la t=c$%ca *#$c%o$a(a- la !#$!%&%l%(a( *@$%*a (# (#t#cc%$ #$ #l "#c#$to #$ placa- t%l%
2. C>mo &e a$i#a e&"e i!di#ado' $a'a e@a%a' a #aidad de ai'e de o& "ae'e& de $'od%##i>! de aime!"o& Lo! al%*#$to! #$t"a$ #$ co$tacto co$ lo! *a! (%2#"!o! *at#"%al#! a lo la"'o (# to(a ! ca(#$a (# p"o(cc%$ J#la&o"ac%$- #$2a!a(o- co$!#"2ac%$- (%!t"%&c%$ y co$!*o- lo 1# %$cly# to(o t%po (# t#$!%l%o! al%*#$ta"%o! J2a%lla- c"%!tal#"@a apa"ato!- #$2a!#! y #*&ala#!- a!@ co*o la! #$2olt"a!- "#2#!t%*%#$to! y co&#"t"a! Jtal#! co*o la! 1# c&"#$ lo! 1#!o!- lo! p"o(cto! (# cha"ct#"@a- o la! )"ta!3 Po" ta$to- cal1%#" *at#"%al  o&#to (#!t%$a(o a #$t"a" #$ co$tacto (%"#cta o %$(%"#cta*#$t# co$ al%*#$to! - ha (# !#" lo !9c%#$t#*#$t# %$#"t# pa"a #2%ta" 1# !#


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t"a$!9#"a$ !!ta$c%a! a lo! al%*#$to! #$ ca$t%(a(#! lo !9c%#$t#*#$t# '"a$(#! pa"a po$#" #$ p#l%'"o la !al( h*a$a o pa"a oca!%o$a" $a *o(%9cac%$ %$ac#pta&l# (# la co*po!%c%$ (# lo! p"o(cto! al%*#$t%c%o! o $a alt#"ac%$ (# la! ca"act#"@!t%ca! o"'a$ol=pt%ca! (# =!to!

-. C>mo &e a$i#a e&"e i!di#ado' $a'a e@a%a' a #aidad de #omida& $'e$a'ada& , $'od%#"o& de $a&"ee'3a

El *=to(o (# "#c#$to #$ placa !# t%l%o y 1#- !%$ #*&a"'o- #8%!ta ca$t%(a( (#t#cta&l# !9c%#$t# (# #$t#"oto8%$a #!ta9locc%ca3 E$ #!t# ca!o- lo! *%c"oo"'a$%!*o! 1# o"%'%$a"o$ la to8%$a ha$ %(o (#!c#$(%#$(o #$ $7*#"o #- %$cl!o(#!apa"#c%#$(o- *%#$t"a! 1# la to8%$a- po" ! *ayo" "#!%!t#$c%ap#"*a$#c# #$ #l al%*#$to3 0. I!di%e #omo &e 'eai+a a $'%ea de a #oa%a&a La p"#!#$t# t=c$%ca pa"a la (#t#cc%$ (# Staphylococc! a"#!- co$!%!t# #$ lo! !%'%#$t#! pa!o!:

03 A%!la*%#$to !#l#ct%2o- !# t%l%a(a! (# Staphylococc! a"#!3 B3 I(#$t%9cac%$ &%o1@*%ca- #$ #!t# p$to !# %(#$t%9ca #l '=$#"o y #!p#c%# (# Staphylococc! a"#! #$t#"oto8%'=$%co3 E!ta! p"#&a! &%o1@*%ca! J(# ac#"(o a la No"*a O9c%al !o$: p"#!#$c%a (# la #$<%*a coa'la!a y p"#!#$c%a (# la #$<%*a t#"*o$cl#a!a3 La


P+'%$a 18


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ca"act#"%
po!%t%2a

po!%t%2a

<. 5I5LIOGRAFIA •

o"'#o%! C3M3 M%c"o&%olo'@a Al%*#$ta"%a3 E(%to"%al Co$t%$#$tal

•

S3A3053 K"a<%#" -3C3 M%c"o&%olo'@a (# lo! al%*#$to! 3E(3 Ac"%&%a 3 E!pa>a ,,,3 Mo!!#l3D3M%c"o&%olo'@a (# lo! al%*#$to!3 E(3 Ac"%&%a3 E!pa>a ,,,3 DIKCO Ma$al (# *#(%o! (# clt%2o3 E(3 D%)co3 MERC3 Ma$al (# *#(%o! (# clt%2o E(3 M#"c- Al#*a$%a3 M%$%!t#"%o (# Sal( DIGESA 3 Ma$al (# A$+l%!%! M%c"o&%ol'%co (#

•

Al%*#$to!3 KAO/ONU/ Ma$al#! pa"a #l co$t"ol (# cal%(a( (# Al%*#$to! pa"a la

• • • •

• •

• • •

• • •

a'"%clt"a y la Al%*#$tac%$3 Ro*a3 http:;;3"#!py$3a$l3*8;2%%;;a"t%clo!;1#!o!Z)"#!co!/03ht* http:;;(#pa3)1%*3$a*3*8;a*y(;a"ch%2#"o;pato'NOMStaphylococc! a"#!Z0B53p() http:;;2%"!3!al3#!;#&;(#*oZ*%c"oal%;Sa"#!;Sa"#!Placa3ht*l http:;;#!3!c"%&(3co*;(oc;.B45;Ma$al/T#c$%co/#!o/Pa"%a http:;;3lact#a3co*3p#;lact#a;%$(#83php[ opt%o$\co*Zco$t#$t]2%#\a"t%cl#]%(\04]It#*%(\5 http:;;#!3%%p#(%a3o"';%%;#!o^#!o!Z)"#!co! http:;;#!3%%p#(%a3o"';%%;#!oZ)"#!co http:;;3p"o*a*a

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P+'%$a 1;



-2014

P+'%$a 2=

NUMERACION DE STAPHYLOCOCUS AUREUS COAGULASA POSITIVO

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