Uji Batas Mikroba

MAKALAH PRAKTIKUM MIKROBIOLOGI UJI BATAS MIKROBA

DISUSUN OLEH:

KELOMPOK 3 DAN 4 Dela Yolanda (2!22!"3# De$%na &'anda (2!22!")# De$%na Ma*l%na (2!22!"+# Enda' Ka,%-a Sa% (2!22!++# .%,% P*,% A/el%a (2!22!!!0# .a'% M*'a/ad (2!22!!1# Ida H%daa,% (2!22!

#

KELAS : B

.AKULTAS .AKULTAS .ARMASI UNIERSITAS PAN&ASILA JAKARTA 2!3

KATA PENGANTAR PENG ANTAR

P*% P*% 5*5*-* * -a/% -a/% 6an 6ana, a,-a -an n -e6a -e6ada da T*'an *'an YME7 YME7 -ae -aena na 8e8e-a, a, a'/ a'/a,9 a,9N Naa -a/% -a/% da6a da6a,, /enele5a%-an Ma-ala' Pa-,%-*/ M%-o8%olo% denan *d*l U% Ba,a5 M%-o8a; Ma-ala' %n% d%5*5*n *n,*- /e/en*'% ,*a5 /a,a -*l%a' Pa-,%-*/ M%-o8%olo% an 8e%5% ,en,an ,e-n%-9,e-n%- an da6a, d%*na-an *n,*- /en'%,*n /%-o8a7 'a5%l 6ena/a,an da% 5e,%a6 -elo/6o- ,en,an /a,e% *% 8a,a5 /%-o8a 5e,a anal%5%5 a, /e/8an*n; Se/oa /a-ala' %n% 8e/an>aa, *n,*- 6ene/8anan %l/* 6ene,a'*an 8a% -%,a 5e/*a;

Ja-a,a7 !! No$e/8e 2!3

Pen*5*n

BAB I PENDAHULUAN

1.1 Latar Belakang

M%-ooan%5/e adala' /a-'l*- an /e/%l%-% *-*an 5ana, -e<%l dan 'ana da6a, d%l%'a, /en*na-an /%-o5-o6; Sala' 5a,* en%5 /%-ooan%5/e adala' 8a-,e%; Ba-,e% /e*6a-an oan%5/e *n%5el*la an ,*/8*' denan a-,o 6enena,95%>a, /%-o8a;Bana- /e,ode an d%*na-an dala/ /ena-5% 5e da% 5*a,*6o6*la5% 8a-,e%; Na/*n7 ada d*a /e,ode an 6al%n 5e%n d%*na-an a%,* /e,ode '%,*n -olon% d% o,o/e,e?,*8%d%/e,e; Seoan Seoan  >a/a5% >a/a5%55 5e8a%5e8a%-na na /a/6* /a/6* /e/6e /e/6ed%d%-5% 5% */la' */la' 5el /%-o8 /%-o8aa dala/ dala/ 5*a,* 5*a,* 5a/6el 5a/6el ,e,en, ,e,en,* * a,a* an d%5e8*, d%5e8*, denan denan cell cell dens densit ityy 5e8el*/ /enanal%5ana; Den5%,a5 5el 5ana, ,ean ,ean,*n ,*n  da% da% en%5 en%5 5a/6le 5a/6le;; .*n5% .*n5% *,a/a *,a/a /e/6e /e/6e-%a -%a-an -an den5%,a den5%,a55 5el %n% adala' adala' *n,*- *n,*- /enen,*-an 6el* a,a* ,%da-na d%la-*-an 6enen
1.2 Rumusan Masalah

a# ! "! #! e!

Me,ode a6a 5aa an d%*na-an *n,*- /en'%,*n /%-o8a@ Baa%/ana /enanal%5%5 *% */la' /%-o8a denan ,e-n%- MPN@ Baa%/ana /enanal%5%5 *% */la' /%-o8a denan ,e-n%- ALT dan AKK@ Baa%/ana /enanal%5%5
1.$ Tu%uan Prakt&kum

a# Me/8*a, 6enen
Se,ela' /ela-*-an 6a-,%-*/ /a'a5%5=a d%'aa6-an /a/6* /ela-*-an ,e-n%- 5e% 6enen
BAB II TIN)AUAN PU*TAKA

Pen*-*an -*n,%,a,%> 6o6*la5% /%-o8a da% 5*a,* 5a/6el d%la-*-an *n,*- /ene,a'*% -*al%,a5 8a'an 8eda5a-an */la' /%-o8a an ada dala/ 5a/6el ,e5e8*,; Se'%na -%,a da6a, /ene,a'*% a6a-a' /%-o8a ,e5e8*, 8e8a'aa a,a* 8a'-an 8a%- 8a% l%n-*nan dala/ */la' ,e,en,*; U% 8a,a5 /%-o8a d%la-*-an *n,*- /e/6e-%a-an */la' /%-o8a aeo8 $%a8le d%dala/ 5e/*a en%5 5a/6le; U% %n% d%la-*-an /*la% da% 8a'an 8a-* 5a/6a% 5a/6le a,a* 5ed%aan ad% *n,*- /ena,a-an 8a'=a 5a/6le ,e5e8*, 8e8a5 da% /%-ooan%5/e 6a,oen ,e,en,* dan 8a,a5 /%-o8ana ,%da- /ele8%'% */la' an d%6e5aa,-an ole' 6ea,*an an 8ela-*; Ada 8e8ea6a /e,ode an da6a, d%*na-an *n,*- /en'%,*n */la' 8a-,e% 5e da% 5*a,* 6o6*la5% 8a-,e%; 1. Perh&tungan )umlah M&kr+a *e"ara Langsung

&aa %n% d%6a-a% *n,*- /enen,*-an */la' /%-o8%a -e5el**'an 8a%- an /a,% /a*6*n an '%d*6; Ada 8e8ea6a
Pe'%,*nan %n% da6a, /e/a-a% 'ae/a<,o/e,e7 Pe,o>>9 Ha*55e Ba<,e%a &o*n,e a,a* ala,9 ala, la%n an 5een%5; Da5a 6e'%,*nanna %ala' denan /ene/6a,-an ! ,e,e5 5*56en5% -*l,* a,a* 8%a-an /%-o8%a 6ada ala, ,e5e8*,7 d%,*,*6 denan ela5 6en*,*6 -e/*d%an d%a/a,% denan /%-o5-o6 an 6e8e5aanna ,ean,*n 6ada 8e5a -e<%lna /%-o8a; Denan /enen,*-an */la' 5el a,a9a,a ,%a6 6e,a- (*anan# an ,ela' d%-e,a'*% $ol*/ena da% ala, ,e5e8*, da6a, d%,en,*-an */la' 5el /%-o8%a ,%a6 <<; . ,ara Penge"atan #an Pengamatan M&kr+sk+-&k

Pada
M*la9/*la d%5a%n 5e*/la' $ol*/e ,e,en,* 5*a,* 5*56en5% 8a'an a,a* 8%a-an /%-o8a -e/*d%an d%5a%n denan >%l,e /e/8an an ,ela' d%5,e%l-an; Denan /en'%,*n */la' 5el a,a9a,a ,%a6 -e5a,*an l*a5 6ada >%l,e /e/8an da6a, d%'%,*n */la' 5el da% $ol*/e 5*56en5% an d%5a%n; Kala* 6e'%,*nan 5e%l,e /e/8an7 -e/*d%an >%l,e /e/8an d%en*'% denan /%na- %/e5% 5*6aa da6a, /enad% ,a56aan;

2. Perh&tungan )umlah M&kr+a *e"ara T&#ak Langsung a. Menggunakan *entr&(uge

&aana %ala' ! << 8%a-an *e denan /e/a-a% 5en,%>*e an 8%a5a d%6a-a% *n,*- /enen,*-an */la' 8*,%98*,% daa'; S*6aa 'a5%lna da6a, d%6e,an*n a=a8-an7 /a-a -e*a5% 'a*5 d%6e'a,%-an; Se,ela' d%-e,a'*% $ol*/e /%-o8a -e5el**'an /a-a da6a, d%6a-a% *n,*- /enen,*-an */la' 5el95e l /%-o8a ,%a6 <<7 a%,* denan /e/8a% $ol*/e /%-o8a -e5el**'an denan $ol*/e a,a9a,a ,%a6 5el /%-o8a; .Ber#asarkan Kekeruhan

Da5a 6enen,*an
Ala, %n% da6a, /enen,*-an 8e%8*9%8* 5el ,%a6 de,%- 5e
&aa %n% d%da5a-an a,a5 'a5%l anal%5a -%/%a 5el95el /%-o8%a; Ma-%n 8ana- 5el95el /%-o8a7 /a-%n 8e5a 'a5%l anal%5a -%/%ana 5e; Yan d%6a-a% 5e8aa% da5a 6enen,*an */*/na %ala' -and*nan 6o,e%n : a5a/9a5a/ n*-lea, (DNA dan RNA# a,a* >o5>o da% a5a/9 a5a/ n*-lea,7 dan 5e8aa%na; e. Ber#asarkan Berat Ker&ng

&aa %n% ,e*,a/a d%*na-an *n,*- 6enen,*an */la' a/* 8enan /%5alna dala/ %nd*5,% /%-o8%olo%; Kena%-an 8ea, -e%n 5*a,* /%-o8%a 8ea,% *a -ena%-an 5%n,e5a dan $ol*/e 5el95el an da6a, d%6a-a% *n,*- /enen,*-an */la' /%-o8a;

(. Menggunakan ,ara Pengen"eran

&aa 6enen
g. Menggunakan ,ara M+st Pr+ale Numer /MPN!

&aa d% a,a5 -*an ,e6a, /en%na, ,%da- 5e/*a /%-o8a da6a, ,*/8*' dala/ 5*a,* /ed%*/ 6ada -eadaan ,e,en,*; Un,*- /ena,a5%na /a-a ,%a6 6enen,a Mo5, Po8a8le N*/8e /%5alna da>,a Ho5-%n5 a,a* da>,a M<;&ad; h. Ber#asarkan )umlah K+l+n& /Plate ,+unt!

&aa %n% an 6al%n */*/ d%6a-a% *n,*- 6e'%,*nan */la' /%-o8a; Da5ana %ala' /e/8*a, 5*a,* 5e% 6enen an d%*na-an *n,*- /ene,a'*% */la' /%-o8a an ada 6ada 5*a,* 5a/6el7 */*/na d%-enal denan An-a Le/6en To,al (ALT#; U% An-a Le/6en To,al (ALT# dan le8%' ,e6a,na ALT aeo8 /e5o>%l a,a* anaeo8 /e5o>%l/en*na-an /ed%a 6ada, denan 'a5%l a-'% 8e*6a -olon% an da6a, d%a/a,% 5e*# 6e /l? a,a* -olon%?!/l; &aa an d%*na-an an,aa la%n denan %l 5e,ela' <*6l%-an d%%no-*la5%-an 6ada/ed%a le/6en aa denan
-e<%l da% d*a 'a5%lna /a-a d% a,a9a,a ,a6% %-a le8%' 8e5a da% d*a an d%6a-a% */la' /%-o8%a da% 'a5%l 6enen
BAB III MET0D0L0GI

$.1 Alat #an Bahan $.1.1 Alat

9 9 9 9 9 9 9 9 9 9 9

Ta8*n9,a8*n ea-5% 5,e%l &a=an 6e,% 5,e%l P%6e, $ol*/e 5,e%l Pe/8a-a B*n5en Ja*/ O5e T%6 /%-o6%6e, ! /L S'a-e o,eC In-*8a,o Elen/ee Ra- ,a8*n ea-5%

$.1.2 Bahan

9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9

Sa/6el an a-an d%anal%5%5 La*,an Da6a .o5>a, 6H "72 La<,o5e Bo,' T6,one So Bo,' Selen%,e &5,%ne Bo,' Te,'a,%ona,e Bo,' B%ll%an, Geen Aa T%6le S*a Ion Aa L5%ne Ion Aa M< &on-e Aa Eo5%n Me,'lene Bl*e Aa Med%a dan 6eea-5% IMI& N*,%en, Aa Pe=ana *n,*- 6e=anaan a/ oel Jo'n5on Aa &e,a/%de Aa Po,a,o DeC,o5e Aa T6,one So Aa

$.2 ,ara Ker%a A. Pers&a-an #an H+m+gen&sas& *am-el

!; Me/8*-a -e/a5an 5a/6el 5e
!; Da% 'a5%l 6e5%a6an dan 'o/oen%5a5% 5a/6el (-on5en,a5% !9!# 8*a,la' 8e8ea6a 5e% 6enena, 5e'%na d%6eole' 6enen
!; S%a6-an !4 ,a8*n 8e%5% + /L /ed%a TSB ; 2; Ba% ,a8*n dala/ 4 -elo/6o-7 -elo/6o- I dan II /a5%n9/a5%n ,ed%% da% 4 ,a8*n7 -elo/6o- III dan I /a5%n9/a5%n ,ed%% da% 3 ,a8*n; 3; P%6e, ! /L la*,an a,a* a, an a-an d% 6e%-5a -e dala/ /a5%n9/a5%n ,a8*n -elo/6o- I 5e'%na d%6eole' 6enen
D. Anal&s&s "emaran m&kr+a -at+gen Escherichia coli

!; B*-a -e/a5an 5a/6le 5e Ko Eo5%n Me,'len Bl*e Aa (EMBA#; In-*8a5% 6ada 5*'* 31 & 5ela/a 2494) a/; A/a,% ada a,a* ,%da-na 6e,*/8*'an -olon% -%la6 loa/ /e,al%-; 1; U% lan*,an ,e'ada6 -olon% an d%d*a Escherichia coli J%-a ,eda6a, -olon% -%la6 loa/ /e,al%- 6ada /ed%a EMBA7 la-*-an *% lan*,an ,e'ada6 -olon% ,e5e8*,; Ino-*la5%-an -olon% ,e5e8*, -edala/ ,a8*n an 8e%5% /ed%a LB 5,e%l denan ,a8*n d*'a/ ,e8al%- dan -edala/ /ed%a aa /%%n NA; In-*8a5% 6ada 5*'* 31& 5ela/a 2494) a/; Da% 'a5%l %n-*8a5%7 la-*-an 6e=anaan a/ dan *% IMI& 0; U% IMI& (Indol7 Me,'len Red7 oe5 Po5-a*e7 S%//on5 &%,a,e# • Indol Ino-*la5%-an 5*56en5% 8a-,e% 5e8ana- ! 5en-el%, -edala / /ed%a %ndol7 %n-*8a5% 5ela/a !)924a/ 6ada 5*'* 3193" &; Se,ela' d%%n-*8a5%7 ,e,e5-an 6eea-5% Ko$a<5 5e8ana- 09) ,e,e5; Ha5%l 6o5%,%> %-a ,e8en,*- la6%5an <%n<%n 8e=ana /ea'  -*n%n  nea,%>#; Un,*- oe59Po5-a*e7 ,e,e5-an 6eea-5% Ba%,5 A (9na>,ol# 3 ,e,e5 dan 6eea-5% Ba%,5 B (KOH 4# ! ,e,e5 -edala/ ,a8*n ea-5% an 8e%5% !?3 8a%an7 'o/oen-an; B%a-an 5ela/a !1/en%, (/ea'  ea-5% 6o5%,%>#; • S%//on5 &%,a,e Ino-*la5%-an 5*56en5% 8a-,e% 5e8ana- !o5e denan #

E. Anal&s&s "emaran m&kr+a -at+gen Salmonella sp. 1. B*-a -e/a5an 5a/6le 5e Ko Ko%- Sal/onella 56; Pada /ed%a aa 5ele-,%> d%,anda% denan adana -olon% 6*,%' d%-el%l%n% ona 6%n-; . U% lan*,an ,e'ada6 -olon% an d%d*a Sal/onella 56; Denan /en*na-an a*/ o5e7 a/8%l -olon% an d%d*a Sal/onnella 56; Da% /ed%a aa 5ele-,%>7 %no-*la5%-an denan %-a: Pada /ed%a TSIA: Leen 8e=ana -*n%n (a5a/# da5a a,a* ,*5*-an 8e=ana /ea' (8a5a# denan a,a* ,an6a enda6an '%,a/ (H2S# Pada /ed%a LIA: Leen dan ,*5*-an 8e=ana *n* (al-al%5?8a5a# denan adana 6e/8en,*-an enda6an '%,a/ (H2S# . Anal&s&s "emaran m&kr+a -at+gen Staphylococcus aureus 1. B*-a -e/a5an 5a/6le 5e Ko%- S,a6'lo d%,anda% denan adana -olon% '%,a/ d%-el%l%n% ona -*n%n . U% lan*,an ,e'ada6 -olon% an d%d*a Staphylococcus aureus (*% -oa*la5e# J%-a ,ead% -olon% '%,a/ d%-el%l%n% ona -*n%n 6ada /ed%a JA7 la-*-an *%a lan*,an ,e'ada6 -olon% ,e5e8*,7 a%,* *% -oa*la5e; A/8%l -olon% dan 6%nda'-an -edala/ ,a8*n an 8e%5% 71/l 6la5/a -el%n<% a,a* -*da; In-*8a5% dala/ 6enana5 a% 6ada

5*'* 3"&7 a/a,% 6ada a/ -e937 07 dan 5e,e*5na 5a/6a% 24a/; J%-a ,eda6a, -oa*la5%7 /a-a 5a/6le d%d*a /enand*n Staphylococcus aureus G. Anal&s&s "emaran m&kr+a -at+gen Pseudomonas aeruginosa

!; B*-a -e/a5an 5a/6le 5e Ko%- Pseudomonas aeruginosa 6ada /ed%a aa 5ele-,%> d%,anda% denan adana -olon% 8e>l*oe5en5% '%a* 4; U% lan*,an ,e'ada6 -olon% an d%d*a Pseudomonas aeruginosa (*% o-5%da5e# J%-a ,ead% -olon% 8e>l*oe5en5% '%a* 6ada /ed%a &e,A7 la-*-an *% lan*,an ,e'ada6 -olon% ,e5e8*,7 a%,* *% o-5%da5e; Le,a--an d%a,a5 -olon% ,e5e8*, a,a* 6%nda'-an -olon% -e -e,a5 5a%n an ,ela' d%en*'-an denan N7N9d%/e,%l969>en%lend%a/%na9 d%'%do-lo%da; J%-a ,ead% 6e*8a'an =ana da% /ea' /*da /enad% le/8a*n7 /a-a 5a/6le d%d*a /enand*n Pseudomonas aeruginosa.

BAB I3 HA*IL PENGAMATAN DAN PEMBAHA*AN

KEL0MP0K 1 /*am-el 4 )amu Kun5&t Asam!

Ha% 6e,a/a Pengen"eran Blan-o !9! !92 !93

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Taung 2

6 6 6

6 6 6

Me#&a La<,o5e Bo,' T6,%< So Bo,'

Taung $

)umlah

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Keterangan Ke*' Ke*'

Ha% -ed*a Anal%5%5 Escherichia coli Me#&a M<; &on-e Aa (M&A# Anal%5%5 Salmonella typhii

Gamar Tela/6%

Keterangan ada -olon% /ea' 8a,a (#

Me#&a Te,'a,%ona,e Bo,' Selen%,e &5,%ne Bo,'

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Keterangan Ke*' (# Ke*' (#

Me#&a oel Jo'n5on Aa (JA# Anal%5%5 Pseudomonas aeruginosa


Keterangan H%,a/ (#

Me#&a &e,%/%de Aa (&e,A#


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Me#&a Eo5%n Me,'lene Bl*e Aa (EMBA# Anal%5%5 Salmonella typhii


Keterangan T%da- -%la6 '%,a/ (9#

Me#&a B%ll%an Geen Aa (BGA#


Keterangan T%da- -%la6 '%,a/ (9#

Anal%5%5 Staphylococcus aureus

Ha% -e,%a Anal%5%5 Escherichia coli

KEL0MP0K 2 /*am-el4 )amu Kun5&t Asam!

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T%6,%
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Tael Pengamatan U%& )umlah M&kr+a #engan tekn&k MPN Kel+m-+k !9!

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MPN 3

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!93

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Tael Pengamatan U%& ,emaran M&kr+a Pat+gen Eschericia coli Me#&a

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Eo5%n Me,'ene Bl*e Aa (EMBA#

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U%& %en&s "emaran m&kr+a Staphyloccocus aureus

Me#&a oel Jo'n5on Aa

Gamar

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Gamar

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KEL0MP0K $ /*am-el 4 *aun ,a&r L&(eu+5!

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Ha5%l

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U% Jen%5 Salmonella 56 Med%a

Selen%,e

Po5%,%> /enand*n /%-o8a7 =ana ,el%'a, -e*' Te,a,'%ona,e Bo,'

U% Jen%5 Staphylococcus aureus Med%a

JA

Ha5%l

Ke,eanan Nea,%> ,%da- ,eda6a, =ana '%,a/ an 8e6%n%an -*n%n;

U% Jen%5 Pseudomonas aeruginosa Med%a

Ha5%l

Ke,eanan Nea,%> ,%da- ,eda6a, =ana '%a* an 8e>l*oe5en5%;

Ha5%l

Ke,eanan Po5%,%> ,eda6a, -olon% 8e=ana 6*,%' d%-el%l%n% ona 6%n-;

&e,A


BGA (Selen%,e#

Po5%,%> ,eda6a, -olon% 8e=ana 6*,%' d%-el%l%n% ona 6%n-;

BGA (Te,a,'%ona,e Bo,'#


TSIA

LIA

Ha5%l

Ke,eanan Leen : -*n%n (a5a/# T%da- ada H2S T%da- ada ele/8*n Leen :  *n* T%da- ada H2S T%da- ada ele/8*n

KEL0MP0K ; /*am-el 4 *&ru- 0at Batuk!

Ta8el 6ena/a,an 6e,*/8*'an 8a-,e% denan ALT (An-a Le/6en To,al# Penen
Ha% -e9!   4 ! 2 1

Ta8el 6ena/a,an 6e,*/8*'an -a6an a,a* -'a/% denan AKK (An-a Ka6an K'a/%# Penen
Ha% -e9!   4 ! 2 1

Ta8el 6ena/a,an 6e,*/8*'an -a6an a,a* -'a/% denan AKK (An-a Ka6an K'a/%# dan 6e,*/8*'an 8a-,e% denan ALT (An-a Le/6en To,al#

(TSB dan LB 5e8el*/ d%%n-*8a5%#

KEL0MP0K < /*am-el 4 *&ru- 0at Batuk!

Ta8el Pena/a,an ALT Penen
Ha% -e9! ! 2 2 4 2 3

Ta8el Pena/a,an AKK

(TSB dan LB 5e,ela' d%%n-*8a5%#

Penen
Ha% -e9! ! 2 2 2+ ! 4

Ta8el Pena/a,an U% Jen%5 M%-o8a

Med%a TSB LB

Ha5%l Pena/a,an (9# T%da- ,ead% 6e*8a'an (9# T%da- ,ead% 6e*8a'an

KEL0MP0K = /*am-el 4 *usu "a&r!

TABEL DATA MPN PENGEN,ERA

TABUNG 1

TABUNG 2

TABUNG $

)UMLAH

N Blan-o

9

9

9









3







3





9

2 !! &.U?/l

Anal&s&s )en&s Me#&a

Keterangan

Lactose Broth

 (-e*'#

Trypticase Soy Broth

 (-e*'#

U%& %en&s "emaran Escherichia coli Me#&a

Keterangan

Mc Conkey Agar

9

U%& %en&s "emaran Salmonella s-.

Me#&a Selenite Cystine Broth Tethrationate Broth Brilliant Green Agar

Keterangan Ke*' Enda6an 6*,%' Selenite Cystine Broth: ,%da- ,eda6a, -olon% Salmonella sp.

Tetrathionate Broth: ,%da- ,eda6a, -olon% Salmonella sp. U%& %en&s Staphyloccocus aureus Me#&a

Keterangan

oel Jo'n5on Aa

9

U%& %en&s Pseudomonas aeruginosa Me#&a &e,%/%de Aa

Keterangan 9

KEL0MP0K 18 /*am-el 4 *usu "a&r! TABEL DATA MPN

Penen
Ta8*n !

Ta8*n 2

Ta8*n 3

J*/la'

MPN?/l

   9

   9

  9 9

3 3 2 9

!!

Se8el*/ %n-*8a5%

U%& )en&s

Se8el*/

Se,ela' %n-*8a5%

Se5*da' Med%a

a-,*

Trypticase Soy Broth (TSB#

4) a/

Ke,eana n Po5%,%>

La<,o5e Bo,' (LB#

4) a/

Po5%,%>

Ha5%l

Anal%5%5 &e/aan M%-o8a Pa,oen Escherichia coli Med%a

Ha5%l

Ke,eanan


Nea,%>7 T%da- ada 6e,*/8*'an an /en*n*--an adana Escherichia coli

Anal%5%5 &e/aan M%-o8a Pa,oen Salmonella sp Se8el*/

Med%a Selen%,e &5,%ne Bo,'

Ha5%l

Ke,eanan Po5%,%>7 Tead% -e*'

Te,'a,%ona,e Bo,'

Po5%,%>7 Tead% -e*'

B%ll%an, Geen Aa (BGA#

nea,%>7 ,%da- ,eda6a, -olon% 6*,%' d%-el%l%n% ona 6%n-

Anal%5%5 &e/aan M%-o8a Pa,oen Staphylococcus aureus Med%a

Ha5%l

Ke,eanan

oel Jo'n5on Aa (JA#

Po5%,%>7 Teda6a, -olon% '%,a/ an /en*n*--an adana Staphylococcus aureus

Anal%5%5 &e/aan M%-o8a Pa,oen Psedomonas aeruginosa Med%a &e,%/%de Aa (&e,A#

PEMBAHA*AN

Ha5%l

Ke,eanan nea,%>7 ,%da- ada >lo*e5en5% '%a* an /en*n*--an adana Psedomonas aeruginosa

!; Pada 6a-,%-*/ -al% %n% d%la-*-an 8e8ea6a /e,ode a%,* /e,ode le/6en ( The Viale Plate Count # dan Me,ode An-a Pal%n M*n-%n?APM ( Most Proale !umer Procedure?MPN#; 2; Me,ode le/6en (The Viale Plate Count #7 6ada /e,ode %n% da6a, d%'%,*n An-a Le/6en To,al?ALT (Total Plate Count # an d%%5,%la'-an *n,*- 8a-,e% dan An-a Ka6an K'a/% d%%5,%la'-an *n,*- -a6an?-'a/%; 3; Me,ode le/6en d%la-*-an denan denan d%,anda% -e-e*'an7 6ada /ed%a Te,a,'%ona,e Bo,' *a d%da6a,-an 'a5%l 6o5%,%> -aena ,eda6a, enda6an 6*,%' ,e,a6% 5e,ela' d%la-*-an *% lan*,an /en*na-an /ed%a B%ll%an, Geen Aa /a-a 'a5%l -ed*ana nea,%>; Hal %n% /en*n*--an ,%da- ,eda6a, 8a-,e% Sal/onella 56; 6ada 5*5* 7 8e%,* *a denan anal%5%5 ;

BAB 3

KE*IMPULAN

!; Da% da,a -elo/6o- 47 6ada 'a5%l ALT ,%da- daa, d%'%,*n -aena */la' -olon% an ,*/8*' ,%da- 5e5*a% denan */la' an 5e'a*5na; Kolon% an 5e'a*5na ,*/8*' 6ada 6enen

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