Phagocytosis in Tetrahymena Sp

Experiment No. 3 Group 3 4BIOLOGY3

Endocytosis 

Endocytosis is a process in which bacteria, like protozoans, acquire nutrients by engulfing a certain molecule in their environment.

Types of Endocytosis Phagocytosis is a type of endocytosis in  which a molecule is engulfed by a bacterium. Pinocytosis

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Phagocytosis

Tetrahymena pyriformis

Objectives of the study 

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Generally, this study aims to observe the process of  endocytosis in single-celled bacteria. Specifically, this study aims to: 

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Observe the phagocytosis of Tetrahymena sp. in graphite shavings Observe the phagocytosis of Tetrahymena sp. in India ink  Compare the number of food vacuoles and the manner of feeding of Tetrahymena sp. in two different food source

Materials and Methods  Materials 

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Tetrahymena sp. cell suspension Chlorella sp. Culture Microscope Slides and Cover Slips Formalin Solution, 3% Filter paper strips

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Microcentrifuge tubes, 1.5ml Micropipettors and tips Graphite shavins from pencil India ink, 1% Toothpicks Pencil Razor blade/cutter

Materials and Methods  Methodology On Graphite Shavings -A drop of  Tetrahymena sp. was suspended in a slide. Shavings of graphite from a lead pencil were introduced to the bacteria on the slide. A cover slip  was placed on the Tetrhymenna sp. culture and graphite. The phagocytocis of the Tetrahymena sp. was observed under the microscope on an HPO.

Materials and Methods On India ink  -An equal amount of the Tetrahymena sp. suspension and 1% India ink was mixed in a microcentrifuge tube. The a small amount of the mixture was pipetted on the slide and  was observed under the HPO objective of the microscope. Comparison -The manner of Tetrahymenna sp.’s feeding and swimming on a graphite shavings and an India ink solution was compared. Food vacuoles of the cells were observed again, under the HPO of the microscope. Number, location, and appearance of food vacuoles and cilia were observed and noted.

Group 1 Cell no.

Tetrahymen Tetrahymen a + graphite a + india ink

1

1

3

2

2

4

 3

1

4

4

2

3

5

1

3

Group 2 Cell No.

Tetrahymena + graphite

Tetrahymena + India ink

1

1

2

2

0

 3

 3

0

2

4

1

2

5

1

 3

average

0.6

2.4

Group 3 CELL NO.

TETRAHYMENA WITH GRAPHITE

TETRAHYMENA WITH INDIAN INK

1

2

2

2

1

1

 3

1

2

4

1

1

5

2

3

 AVERAGE

1.6

1.8

Group 4 Cell Number 1

Tetrahymena + Graphite

Tetrahymena + India Ink

1

4

2

1

3

 3

0

2

4

1

2

5

1

2

 A

Group 7 Tetrahymena sp. Tetrahymena sp.

Tetrahymena sp.

+ India ink + 3%

+ Graphite

+ India ink

Formalin

Cell No.

Solution 1

1

3

3

2

2

2

2

 3

1

3

2

4

1

3

3

5

1

2

3

Group 8 Cell no.

Tetrahymen Tetrahymen Tetrahymen a + graphite a + india ink a + formalin

1

0

3

2

2

1

3

1

 3

0

2

1

4

1

2

2

5

1

2

2

Cell Tetrahymena + Graphite Group 9

Tetrahymena + India Ink

Number 1

2

2

2

3

1

 3

0

2

4

2

2

5

0

1

 Average

1.4

1.6