Bioteknologi Farmasi (Pendahuluan) Soni Muhsinin, M.Si
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Aturan Perkuliahan 1. No HP No Ngobrol 2. Interaksi ksi 2 Ar Arah 3. 100% 100% akt aktif if dalam dalam ber berta tan nya dan dan menja menjaw wab 4. Terla erlamb mbat at maks maks 10 meni menitt
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Kontrol Kontrol Keaktifan
Nama Lengkap
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Sistem Penilaian Ujian (70%) + Keaktifan Keaktifan (30%)
Nilai
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Materi •
Pendahuluan
•
Protein dan DNA
•
Replikasi, Transkripsi, dan Translasi
•
Teknologi eknolog i DNA Rekombinan
•
Over Ekspresi gen
•
Over Produksi dan purifikasi protein rekombinan rekombinan
•
Protein Terapeutik
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Definisi Bioteknologi Bios → Hidup Teuchos → Alat Logos → Ilmu
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BIOTEKNOLOGI
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SEJARAH PERKEMBANGAN BIOTEKNOLOGI • • •
3000 th SM 1680 1818 1857
minuman beralkohol beralkohol hasil fermentasi fermentasi penemuan sel khamir oleh Antonie van Leeuwenhoek fermentasi fermentasi sel khamir oleh Erxleben fermentasi fermentasi laktat oleh Pasteur
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•
1961 1961-1966 1961-196 6 1970
•
1972
•
1973
• •
Jurnal Biotechnology and Bioengineering ditetapkan Seluruh sandi genetik terungkap Nathan & Smith: enzim restriksi endonuklease (enzim yang dapat memotong molekul DNA secara spesifik) pertama kali diisolasi Korona dkk. Berhasil mensintesa secara kimiawi seluruh gen tRNA Boyer dan Cohen memaparkan teknologi DNA rekombinan
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•
1982
•
1883
•
1988
Untuk pertama kalinya kalinya vaksin hewan hasil teknologi DNA rekombinan disetujui pemakaiannya di Eropa Plasmid Ti hasil rekayasa rekayasa genetik dipakai untuk transformasi tanaman US Pat Patent ent diberikan untuk mencit hasil rekayasa rekayasa genetik sehingga rentan terhadap kanker (untuk
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PETA PET A KONSEP Kelangsungan Kelangsungan Hidup Manusia Ditunjang Oleh
Teknologi
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BIOTEKNOLOGI
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KULTUR JARINGAN Dapat dilakukan pada jaringan tanaman maupun hewan Contoh: Kultur jaringan tanaman secara secara in vitro Dasar Teori : Sifat Totipotensi tanaman Artinya setiap bagian tanaman apabila dikulturkan secara in vitro akan dapat membentuk individu baru •
Keuntungannya: 1. sifat tanaman sesuai dengan induknya
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PROSES PENANAMAN EKSPLAN
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RUANG INKUBASI KULTUR
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CONTOH TANAMAN HASIL PERBANYAKAN SECARA IN VITRO
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CONTOH TANAMAN HASIL PERBANYAKAN IN VITRO
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REKA REK AYASA GENE GENETIK TIKA A Rekayasa genetika atau teknologi DNA recombinant DNA technology ) rekombinan (recombinant
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Taha ahapan pan Rek Rekayasa ayasa Genet Genetika ika •
Bahan yg diperlukan : Molekul DNA spesifik yang dikehendaki Vektor (pembawa DNA) ▪
▪
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KONTRIBUSI/TUJUAN REKAYASA GENETIKA Melakukan studi tentang struktur & fungsi gen (analisis (analisis gen) • Amplifikasi produk suatu gen gen dalam keadaan keadaan murni
•
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Produk Bioteknolohi Molekuler SEKTOR : Medicinal : Human Therapeutic
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Diagnostik Molekuler
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Untuk mengatasi kendala kendala kendala tersebut maka dikembangkan prosedur prosedur diagnostik –
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MENINGKA MENING KATKAN TKAN PRODUKSI ANTIBOTIK
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Terap erapii Gen Gen
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Dasar pemikiran terapi gen:
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