Laporan Penentuan Potensi Antibiotika

LAPORAN PRAKTIKUM MIKROBIOLOGI FARMASI FARMASI PENENTUAN POTENSI ANTIBIOTIKA

Rabu, 6 Mei 2015 Kelompo !" Rabu, Puul 1#$#0 % 16$#0 &IB

Nama

NPM

Tugas

Ku'(ia Me)a*a+i

2601101#0122

Ima- Laili Le-+a'i

2601101#012#

Tuua(, P'i(-ip, Ala+ Ba.a(,P'o-e/u', Pe'.i+u()a(, Pemba.a-a( /i+o', Teo'i a-a', a-il a+a Pe()ama+a(, Pe'.i+u()a(, Pemba.a-a(, Simpula(

LABORATORIUM MIKROBIOLOGI FARMASI FAKULTAS FARMASI UNI"RSITAS PA3A3ARAN 2015

 Nilai

TT

4S.i(+a Noo' Amala Amala 4Be(e/i7+u-Ge(+a 4Be(e/i7+u-Ge(+a P

I.TUJUAN

Me(e(+ua( be-a'(a po+e(-i -ampel a(+ibio+ia /i pa-a'a( +e'.a/ap a(+ibio+ia -+a(/a'$

II.

PRINSIP 1$ Pe()e(7e'a( A(+ibio+ia

Pe()e(7e'a( a(+ibio+ia /ilaua( u(+u membe'ole. 8a'ia-i o(-e(+'a-i a(+ibio+i7$ Pe()e(7e'a( /ilaua( /e()a( me())u(aa( pela'u+ be'upa aua/e-+e'il /a( /ilaua( -e7a'a a-ep+i-$ 2$ Po+e(-i A(+ibio+ia Ui po+e(-i a(+ibio+ia -e7a'a mi'obiolo)i a/ala. -ua+u +e(i u(+u  me(e+apa( -ua+u po+e(-i

a(+ibio+ia /e()a( me()uu' e9e -e(a*a +e'-ebu+

+e'.a/ap pe'+umbu.a( mi'oo')a(i-me ui a() pea /a( -e-uai$ 9e a() /i+imbula( pa/a -e(a*a ui /apa+ be'upa .amba+a( pe'+umbu.a($ #$ :o(a amba+ Te'be(+u(a ;o(a be(i() a+au ;o(a .amba+ me(a(/aa( a/a(a po+e(-i /a'i a(+ibio+i a() /i)u(aa( /alam me().amba+ /a( membu(u. ba+e'i$ <$ Te(i a-ep+iTe(i a-ep+i- ai+u +e(i a() /i)u(aa( /alam pe(7e)a.a( o(+ami(a-i -elama membua+ /a( me(-+e'ila( me/ium ul+u'$ Ti(/aa( a-ep+i- i(i be'+uua( u(+u me()u'a()i a+au me().ila()a( mi'oo')a(i-me a() +e'/apa+ pa/a  pe'muaa( be(/a .i/up a+au be(/a ma+i$ 5$ Me+o/e lempe() Me+o/e lempe() /i/a-a'a( pa/a /i9u-i a(+ibio+ia /a'i -ili(/e' a+au luba() a() a() /ipa-a() +e)a lu'u- pa/a lapi-a( a)a' pa/a+ /alam 7a*a( pe+'i a+au lempe(), -e.i())a mi'oba a() /i+amba.a( /i.amba+ pe'+umbu.a((a$ Pe().amba+a( pe'+umbu.a( mi'oba i(i aa( +ampa -eba)ai /iame+e' /ae'a.

.amba+ 4$ III.

TEORI DASAR  

A(+ibio+ia a/ala. ;a+=;a+ imia a() /i.a-ila( mi'o=o')a(i-me .i/up +e'u+ama 9u()i /a( ba+e'i +a(a., a() memilii .a-ia+ mema+ia( a+au me().amba+  pe'+umbu.a( ba(a ba+e'i /a( bebe'apa 8i'u- be-a', -e/a()a( +o-i-i+a-(a ba)i ma(u-ia 'ela+i9 e7il 4Ta, 200>$ A(+ibio+i a/ala. -e)olo()a( -e(a*a, bai alami maupu( -i(+e+i, a() mempu(ai e9e me(ea( a+au me().e(+ia( -ua+u p'o-e bioimia /i /alam o')a(i-me, .u-u-(a /alam p'o-e- i(9e-i ole. ba+e'i 4?'ai)$, 1@@>$ Be'/a-a'a( -i9a+(a a(+ibio+i /iba)i me(a/i /ua a(+ibio+i a() be'-i9a+  ba+e'i-i/al, ai+u a(+ibio+i a() be'-i9a+ /e-+'u+i9 +e'.a/ap ba+e'i /a( a(+ibio+i  a() be'-i9a+ ba+e'io-+a+i, ai+u a(+ibio+i a() bee'a me().amba+ pe'+umbu.a( a+au mul+iplia-i ba+e'i 4Sae(e, e+ al 2005$ Be'/a-a'a( mea(i-me e'a(a /apa+ /iba)i me(a/i 5 elompo ai+u  pe())a())u me+aboli-me -el mi'oba 4-ul9o(ami/, +'ime+op'i(, a-am p=ami(o-ali-ila+ 4PAS, /a( Sul9o($, pe().amba+ -i(+e-i- /i(/i() mi'oba 4pe(i-ili(, -e9alo-po'i(,  ba-i+'a-i(, 8a(omi-i(, /a( -ilo-e'i(, pe())a())u pe'meabili+a- memb'a( -el mi'oba 4polimi-i(, )olo()a( polie( -e'+a be'ba)ai a(+imi'oba emo+e'apeu+i  pe().amba+ -i(+e-i- p'o+ei( -el mi'oba 4)olo()a( ami(o)lio-i/, ma'oli/, li(omi-i(, +e+'a-ili(, /a( lo'am9e(iol, pe().amba+ -i(+e-i- a+au me'u-a a-am (ulea+ -el mi'oba 4'i9ampi-i(, /a( )olo()a( ui(olo( 43a*e+; et.al $ 2005$ Se7a'a umum a(+ibio+ia +e'ba)i a+a- 4Ta e+ al, 200>$ C Pe(i-ili( Pe(i-ili(=G /a( +u'u(a((a be'-i9a+ ba+e'i-i/ +e'.a/ap +e'u+ama uma( G'am=po-i+i9  4.u-u-(a ?o77i /a( .a(a bebe'apa uma( G'am=(e)a+i9$ ?o(+o.(a  Be(;ilpe(i-ili(, Fe(o-ime+ilpe(i-ili( Klo-a-ili(, A-am Kla8ula(a+, Ampi-ili($ C Se9alo-po'i( Spe+'um e'a(a lua- /a( melipu+i ba(a uma( G'am=po-i+i9 /a( G'am=(e)a+i9  +e'ma-u  Escherichia coli$ Be'.a-ia+ ba+e'i-i/ /alam 9a-e pembu(u.a( uma(,

 be'/a-a'a( pe().amba+a( -i(+e-a pep+i/o)lia( a() /ipe'lua( uma( u(+u  e+a())u.a( /i(/i()(a$ ?o(+o.(a  Se9ale-i(, Se9ama(/ol, Se9ou'o-i(, Se9o+a-im, Se9+a;i/im, A;+'eo(am$ C Ami(o)lio-i/a A+i8i+a-(a ba+e'i-i/, be'/a-a'a( /aa(a u(+u mempe(e+'a-i /i(/i() ba+e'i /a( me()ia+ /i'i pa/a 'ibo-om /i /alam -el$ P'o-e- +'a(-la-i 4RNA /a( NA /i)a())u -e.i())a bio-i(+e-a p'o+ei((a /ia7aua($ 9e i(i +i/a -aa +e'a/i pa/a 9a-e pe'+umbu.a( u)a bila uma( +i/a membela. /i'i$ ?o(+o.(a  S+'ep+omi-i(, Ge(+ami-i(, Ami-i(, Neomi-i( Pa'omomi-i($ C Te+'a-ili( Mea(i-me e'a be'/a-a'a( /i)a())u(a -i(+e-a p'o+ei( uma($ Spe7+'um e'a(a lua- /a( melipu+i ba(a 7o77i G'am=po-i+i9 /a( G'am=(e)a+i9 -e'+a eba(aa(  ba7illi, e7uali p-eu/omo(a- /a( p'o+eu-$ ?o(+o.(a  Te+'a-ili(, o-i-ili(, C Ma'oli/a /a( li(omi-i( 'i+'omi-i( bee'a ba+e'io-+a+i- +e'.a/ap +e'u+ama ba+e'i G'am=po-i+i9, /a( -pe7+'um e'a(a mi'ip pe(i-ili(=G$ Mea(i-me e'a(a melalui pe()ia+a( 'e8e'-ible pa/a 'ibo-om uma(, -e.i())a -i(+e-i- p'o+ei((a /i'i(+a()i$ ?o(+o.(a  'i+'omi-i(, A;i+'omi-i(, Spi'ami-i(, Li(omi-i($ C Polipep+i/a K.a-ia+(a

a/ala.

ba+e'i-i/

be'/a-a'a(

a+i8i+a-

pe'muaa((a

/a(

emampua((a u(+u melea+a( /i'i pa/a memb'a( -el ba+e'i, -e.i())a  pe'meabili+a- -el me(i()a+ /a( a.i'(a -el mele+u-$ ?o(+o.(a  Polimi-i( B, Ba-i+'a-i(, G'am-i/i($ C A(+ibio+ia lai((a K.a-ia+(a be'-i9a+ ba+e'io-+a+i- +e'.a/ap enterobacter /a(  Staphylococcus aureus  be'/a-a'a( pe'i(+a()a( -i(+e-a polipep+i/a uma($ ?o(+o.(a  Klo'am9e(iol, "a(omi-i(, A-am 9u-i/a+, Mupi'o-i(, Spe+i(omi-i( 4Ta e+ al, 200>$ Sua+u ;a+ a(+imi'oba a() i/eal, memilii +o-i-i+a- -ele+i9$ I-+ila. i(i be'a'+i  ba.*a -ua+u oba+ be'ba.aa ba)i pa'a-i+ +api +i/a memba.aaa( ba)i i(a()$

Umum(a +o-i-i+a- -ele+i9 lebi. be'-i9a+ 'ela+i9 /a( bua( ab-olu/, i(i be'a'+i  ba.*a -ua+u oba+ a() pa/a o(-e(+'a-i +e'+e(+u /apa+ /i+ole'a(-i ole. i(a() umum /apa+ me'u-a pa'a-i+ 4Ta e+ al, 200>$ Ri9ampi-i( me'upaa( -e(a*a a(+imi'oba a() -ampai -aa+ i(i ma-i. me(a/i  pili.a( -eba)ai oba+ a(+i TB 4Tube'7ulo-i-$ alam -e/iaa(, 'i9ampi-i( -e'i() /iombi(a-ia( /e()a( IN /a( e+ambu+ol u(+u me(7apai e9e 9a'maolo)i a() lebi. bai$ Be(+u -e/iaa( a() ba(a /i+emua( /ipe'/a)a()a( umum(a +able+, ap-ul a+au aple+, bai +u())al maupu( ombi(a-i$ 9e 9a'maolo)i 'i9ampi-i( -eba)ai a(+i +ube'ulo+i be'la()-u() melalui mea(i-me e'a pe().amba+a(  polime'a-e RNA a() be')a(+u() pa/a NA ba+e'i$ Spe+'um e'a(a lua-, /i-ampi() +e'.a/ap mioba+e'i, u)a e9e+i9 +e'.a/ap -eumla. ba+e'i )'am po-i+i9  /a( (e)a+i9 4Mu+-7.le', 1@@6$ alam la'u+a( ba-a 'i9ampi-i( mu/a. +e'o-i/a-i /e()a( a/a(a o-i)e( a+mo-9e'$ Rea-i i(i /apa+ /i7e)a. /e()a( pe(amba.a( (a+'ium a-o'ba+ -eba)ai a(+i o-i/a($ i-impa( /alam *a/a. +i/a +embu- 7a.aa, +e'+u+up 'apa+ +e'li(/u() /a'i  pa(a- be'lebi.a( 4Flo'e, 1@D6$ Sua+u a(+ibio+ia pe'lu /i+e(+ua( po+e(-i(a a'e(a e9e pe())u(aa( a(+imi'oba a() me(i()a+, -e.i())a me(i()a+a( pula e9e 'e-i-+e(-i be'ba)ai mi'oba pa+o)e($ 9e+i8i+a- /aa .amba+ a+au /aa bu(u. a(+imi'oba -a()a+ +e')a(+u() pa/a umla. /a( eua+a( ;a+ a+i9(a 4Si())i., 200D$ Me+o/e umum /alam ui po+e(-i a(+ibio+i a(+a'a lai(  1$ Me+o/e lempe() 4-ili(/e'Ee'+a- 7a'am Me+o/e i(i /i/a-a'a( pa/a /i9u-i a(+ibio+i /a'i -ili(/e' a() /ipa-a() +e)a lu'u pa/a lapi-a( a)a' pa/a+ /alam 7a*a( pe+'i a+au lempe() a() be'i-i biaa( mi'oba ui pa/a umla. +e'+e(+u$ Se/iaa( a(+ibio+ia me().amba+ pe'+umbu.a( mi'oba a() a/a pa/a lempe() a)a' 4Si())i., 200D$ 2$ Me+o/e +u'bi/ime+'i amba+a( pe'+umbu.a( biaa( mi'oba /alam la'u+a( -e'ba-ama a(+ibio+i, /alam me/ia 7ai' a() /apa+ me(umbu.a( mi'oba /e()a( 7epa+ bila +i/a +e'/apa+

a(+ibio+i me+o/e +u'bi/ime+'i /ilaua( pa/a -ampel a() -uli+ la'u+ /alam ai', 7o(+o.(a  )'ami-i/i( 4Si())i., 200D$  Bacillus subtilis a/ala. mi'oo')a(i-me +a(a. /i ma(a=ma(a a() membe'ia( o(+'ibu-i u(+u .a'a be'-epe/a e+ia biolo)i- a+i9 a'e(a be'ba)ai e(;im a() /i.a-ila( ole. a())o+a -pe-ie-$ Me-ipu( a()a a() -ebe(a'(a a() a/a /i li()u()a( u(+u +.i--pe7ie- belum /i+e(+ua(, ba-il +e'a/i pa/a +i()a+ popula-i 106=10D pe' )'am +a(a.$ Namu(, e7uali +a(a. +ela. /iuba. +e'a.i' /e()a( ba.a( o')a(i membe'ia( (u+'i-i mu/a. /apa+ /ipaai, ba-il a/a /i +a.ap e(/o-po'a$ ipe'i'aa( ba.*a 60 -ampai 100 /a'i popula-i ba-il +a(a. a/a /i (e)a'a -po'a a+i9 4Alea(/e', 1@DD$ Sepe'+i eba(aa( a())o+a )e(u-, B$ -ub+ili- a/ala. ae'obi, e7uali /e()a( a/a(a )luo-a /a( (i+'a+, bebe'apa pe'+umbu.a( a(ae'ob /apa+ +e'a/i 4?lau- /a( Be'ele, 1@>6$  Bacillus me'upaa( ba+e'i G'am po-i+i9, be'be(+u ba+a(), bebe'apa -pe-ie be'-i9a+ ae'ob obli)a+ /a( be'-i9a+ a(ae'obi 9aul+a+i9, /a( memilii e(/o-po'a -eba)ai -+'u+u' be'+a.a( -aa+ o(/i-i li()u()a( +i/a me(/uu()$ Me(u'u+ Fa'/ia; 41@@2 be(+u -po'a 4e(/o-po'a  Bacillus be'8a'ia-i be')a(+u() pa/a -pe-ie-(a$ (/o-po'a a/a a() lebi. e7il /a( a/a u)a a() lebi. be-a' /a'i pa/a /iame+e' -el i(/u(a$ Pa/a umum(a -po'ula-i +e'a/i bila ea/aa( me/ium membu'u, ;a+=;a+ a() +imbul -eba)ai pe'+ua'a( ;a+ a() +e'aumula-i /a( 9a+o' lua' lai((a a() me'u)ia( 4Ba7ma( e+ al$,1@@<$

Sumbe' )amba' .++pEEa(abio$8(E)ioi=+.ieu=loi=.ua(=ba7illu-=-ub+ili-E

IV.

ALAT DAN BAHAN IV.1 Alat a$ ?a*a( pe+'i  b$ I(uba+o' 7$ 3a()a -o'o() /$ Labu uu' 100 ml e$ Lampu -pi'+u9$ Mi'opipe+ )$ Mo'+i' /a( -+am9e' .$ Pe'9o'a+o' i$ Pi(-e+  $ Ra +abu() $ Spa+el l$ Tabu() 'ea-i be-a' m$ "olume pipe+ be'uu'a( 1 ml /a( 10 ml IV.2 Bahan a$ Ai' -uli() -+e'il  b$ La'u+a( /e-i(9e+a( 7$ Me/ia (u+'ie( a)a' /$ Pela'u+ -e/iaa( ui e$ Se/iaa( a(+ibio+ia -+a(/a' /a( -ampel 4Ri9ampi-i( 9$ Su-pe(-i ba+e'i Bacillus subtilis IV. !am"a# Alat

?a*a( pe+'i

3a()a -o'o()

Labu uu' 100 ml

Lampu -pi'+u-

Mi'opipe+

Mo'+i' /a( S+ampe' 

Pe'9o'a+o' 

Pi(-e+

Ra Tabu()

Spa+el

Tabu() 'ea-i be-a' 

"olume pipe+ 1 ml

"olume pipe+ 10 ml

V. PROSEDUR 

Su-pe(-i ba+e'i /i-iapa( /alam Nu+'ie( b'o+. a() be'umu' 1>=2< am,  ba+e'i i(i .a'u- .omo)e($ Pe'be(i.a( (u+'ie( a)a' /e()a( 7a'a mela'u+a( -eumla. +e'+e(+u (u+'ie( a)a' /alam aHua/e- emu/ia( /i-+e'ila( /alam o+ola9 -elama 15 o me(i+ pa/a 121 ?$ Se/iaa( ui /ima-ua( e /alam labu uu', /ila'u+a( /e()a(

-e/ii+ pela'u+(a$ Kemu/ia( /i+amba.a( ai' -uli() -+e'il -ampai +a(/a ba+a-$ 3ia -e/iaa( ui be'be(+u pa/a+, )e'u- /a.ulu /alam mo'+i', -ebelum /ima-ua( /alam labu uu'$ Pe()e(7e'a( la'u+a( -ampel /a( la'u+a( -+a(/a' /i'e(7a(aa( .i())a /i/apa+ 8a'ia-i /ua -e'i /o-i- a() /ii()i(a( 4/o-i- +i())i, /o-i- me(e()a. /a( /o-i'e(/a.$ La'u+a( i(oulum /ibua+ /e()a( 7a'a mema-ua( -u-pe(-i biaa( ba+e'i e /alam (u+'ie( a)a' a() +ela. /i-+e'ili-a-i$ alam ea/aa( ma-i. 7ai', (u+'ie( a)a'  a() +ela. me()a(/u() -u-pe(-i ba+e'i +e'-ebu+ /i+ua()a( e /alam 7a*a( pe+'i -e7a'a a-ep+i- -eba(a 20 ml$ ibia'a( -ampai membeu$ Pe'muaa( /a-a' 7a*a( me(a/i e(am a'ea -ama be-a'$ ibe'i label ma-i()=ma-i() a'ea +e'-ebu+ +e')a(+u() 8a'ia-i -e'i /o-i- a() aa( /i)u(aa($ (am 7e+aa( 'e-e'8oi' 4luba() /ibua+ pa/a ma-i()=ma-i() 7a*a( pe+'i /e()a( me())u(aa( pe'9o'a+o' -e7a'a a-ep+i-$ Re-e'8oi'  +e'-ebu+ /ibua+ /e()a( 7a'a membua() a)a' a() a/a /alam 7e+aa( 'e-e'8oi'  +e'-ebu+ /e()a( me())u(aa( -pa+el$ a-il bua()a( +e'-ebu+ /ima-ua( e /alam la'u+a( /e-i(9e+a( a() +ela. /i-e/iaa($ La'u+a( -ampel /a( -+a(/a' /ima-ua(  pa/a ma-i()=ma-i() 'e-e'8oi' -e-uai /o-i- a() /i+e(+ua( /e()a( me())u(aa( o mi'opipe+ -e7a'a a-ep+i-$ Kemu/ia( /ii(uba-ia( /alam i(uba+o' pa/a -u.u #D ? -elama 1>=2< am$ iame+e' /ae'a. be(i() 4;o(e li-i- a() +e'a/i /i -eelili() 'e-e'8oi' a() +ela. me()a(/u() a(+ibio+ia +e'-ebu+ /iuu' /e()a( me())u(aa(  a()a -o'o() /a( /i7a+a+$ Lalu po+e(-i a(+ibio+i7 /i.i+u()$

DA$TAR PUSTAKA

Alea(/e', M$ 1@DD$ Introduction to Soil Microbiology. Ne* o'  3o.( &ile a(/ So(-, I(7$

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