PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
INTEGRANTES: • • • • •
CASTRO BUITRÓN, DIEGO ARMANDO DÍAZ MINCHAN, ROBERTO ANTONIO FERNÁNDEZ REBAZA, GUSTAV GUSTAVO ADOLFO FLORES CHOQUE, POOL HAROL GARAMENDEZ CASTILLO, EDSON BRUNO
PROFESORA RESPONSABLE: •
Mg. YADIRA YADIRA FERNÁNDEZ FERNÁNDE Z JERÍ JE RÍ
DÍA LUNES LUNES MESA: N ! " DÍA
Facultad de Farmacia y Bioquímica Página 0
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA INTRODUCCIÓN
L$ %&'()$ *$+$- %/0(1-g%&$/$ %/ 2&$ %&'()$ NAD %3%&(%&% 42% /% %&+2%&1$ $)3*($)%&% (/1(52($ %& %6(-/ $&()$*%/, 7%g%$*%/ 8 %& )(+1--1g$&(/)-/. E/ 2&$ %&'()$ +(-3*$/)9(+$ 42% /% %&+2%&1$ %& -1)$ /-*25*% - *(g$$ $ )%)51$&$. L$/ (%1%&%/ (/-%&'()$/ /% %&+2%&1$& %& (/(&$/ 31-3-1+(-&%/ %& +$$ %6(-, *- +2$* 32%% $1 *$ (%$ % *$ 2&+(-&$*($ % +$$ (/-%&'()$, $%&(%&- $ *$/ 2&+(-&%/ )%$5;*(+$/ 42% (&%17(%&%&, 3-1 $&- 62%g$ 2& 3$3%* ()3-1$&% %& %* )%$5-*(/)- % g*<+(-/, (&%17(&(%&- %& %* <*()- 3$/- % *$ g*2+-*(/(/ $&$%1;5(+$ 8 %& 31()%1 3$/- 0$+($ *$ g*2+-&%-g=&%/(/ g*2+-&%-g=&%/(/ $ 3$1(1 %* *$+$-, *$+$-, 3-1 %/- (%&((+$1%) (%&((+$1%)-/ -/ *$ $+(7($ % *$ %&'()$ *$+$- %/0(1-g%&$/$, %& (%1%&%/ %6(-/ $&()$*%/. P-1 -1$ 3$1% %* +(-+1-)- ->($/$ +$$*('$ *$ ->($+(;& +-&/%+2(7$ % +2$1- +(-+1-)-/ + +-& *$ +-&+-)($&% 1%2++(;& % 2& ->(g%&- )-*%+2*$1. E* +-)3*%6- (%&% 2& 1%g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g%&$/$ +$$*('$ *$ 1%$++(;& -&% %* /2++(&$- /% ->($ $ 2)$1$-, %/$ %/ 2&$ %&'()$ +-)3*%6$ 42% /% 0$**$ %/1%+0$)%&% 2&($ $ *$ )%)51$&$ (&%1&$ )(-+-&1($*. E/$ %&'()$ %/ 2& %6%)3*- ?3(+- % *$7-31-%(&$/ %& *$ 42% *-/ %*%+1-&%/ 8 31--&%/ /% 1$&/(%1%& %/% %* /2/1$-, %* /2++(&$-, $ 1$7=/ %* FAD FAD 2&(+-7$*%&%)%&% 8 *-/ +%&1-/ %11-/2*21$-/ %& *-/ 42% %* 0(%11- &- 0%)- %>3%1()%&$ ->($+(;& 8 1%2++(;&. L-/ %*%+1-&%/ /% 1$&/(%1%& (&$*)%&% $* +-%&'()$ Q 3$1$, $ +-&(&2$+(;&, /%1 1$&/3-1$-/ 3-1 *$ +$%&$ % 1$&/%1%&+($ %*%+1;&(+$, 3-1 $&-, + (%&((+$1%)-/ *$ $+(7($ % *$ %&'()$ /2++?&(+- %/0(1-g%&$/$, )%($&% %* 2/- % $+%3-1%/ %*%+1;&(+-/ +-*-1%$-/ 8 (%&((+$1%)-/ %* %%+- (&0(5(-1(- % $*g2&-/ /2/$&+($/ /-51% *$ +$%&$ 1$&/3-1$-1$ % %*%+1-&%/.
MARCO TEÓRICO Facultad de Farmacia y Bioquímica Página 1
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA Lactato deshidrogeasa L$ %&'()$ *$+$- %/0(1-g%&$/$ @LDH %/ 2&$ ->(-11%2+$/$ 42% ->($ %* *$+$- $ 3(127$-. C-& %**- /% 1$&/(%1% -/ %*%+1-&%/ $* +-/2/1$- &(+-(&$)($ $%&(&$ (&2+* (&2+*%- %-((- @NAD @NAD,, 1%2+(= 1%2+(=&-* &-*-- $ NAD NADH. H. L$ 1%$++(; 1%$++(;&& 1$&/+ 1$&/+211% 211% 31()%131()%1- %& *$ (1%++(;& +-&1$1($# %* 3(127$- +-)- 31-2+- (&$* % *$ g*2+-*(/(/ /% 1%2+% $ *$+$- %& +-&(+(-&%/ $&$%1;5(+$/, % )-- 42% /% -5%&g$ %* NAD &%+%/$1(- 3$1$ %g1$$1 *$ g*2+-/$. L$ %&'()$ +$$*('$ % (g2$* )$&%1$ *$ 1%$++(;& (1%+$ 8 *$ (&7%1/$# /()3*%)%&% $+%*%1$ *$ -5%&+(;& %* %42(*(51(- % *$ 1%$++(;& /(& (&*2(1 %& *$ 3-/(+(;& % %/% %42(*(51(-. E* *$+$- /% 2&% $ *$ %&'()$ /-*- %& 31%/%&+($ %* +-/2/1$- NAD. E>(/%, 3-1 *- $&-, 2&$ /%+2%&+($ -1%&$$ % 2&(;&: 31()%1- 2&% %* NAD 8 %/32=/ %* *$+$- %& %* +%&1$+(7 $+(7-. -. U& %/3* %/3*$' $'$) $)(% (%& &-- %& *$ +$% +$%&$ &$ 3%3 3%3? ?(+$ (+$ % *$ LDH LDH % 2& 2&$$ *-&g( *-&g(2 2 % $31->()$$)%&% ! $)(&-9+(-/ %>3%1()%&$, 1$/ *$ 2&(;& % *-/ /2/1$-/, 2& $)3*(+$)5(- % +-&-1)$+(;& +%11$&- %* +%&1- $+(7-# )2+0$/ %&'()$/ +2%&$& +-& 2&$ 32%1$ 1$)3(**$ +-)- %/$. E/- %>+*28% %* $g2$, +-& *- 42% 3-%&+($ *$/ (&%1$++(-&%/ %*%+1-/9(+$/ %& %* +%&1- $+(7- 8 )$&(%&% $* $g2$ *%6-/ % *-/ 1%$+$&%/. A%)9/, %* 0%+0- % %>+*2(1 %* +%&1- $+(7- 3%1)(% $ *$ %&'()$ $51$'$1 3-1 +-)3*%- %* %/$- % 1$&/(+(;& % *$ 1%$++(;& 42% %/9 +$$*('$&-. E* +%&1- $+(7- 2&% *$+$- )%($&% 2& 32%&% /$*(&- %&1% *$ +$%&$ *$%1$* % $1g(&(&$ !! @%&'()$ 8 %* g123- +$15->(*+$15->(*- @/2/1$-, $/? +-)- $ 1$7=/ % 2& 32%&% % 0(1-g%&%&1% 0(/((&$! 8 %* g123- 0(1->(*- %* *$+$-, 8 /(<$ $/? %* /2/1$- % )$&%1$ ;3()$ +-& 1%/3%+- $* +-/2/1$- NAD. L$ 0(/((&$! $+<$ % %/$ -1)$ +-)- +$$*('$-1 59/(+-: %& %* %/$- % 1$&/(+(;& 1$&/ (+(;& %>1$% %>1 $% %* % * 31-;& %* g123g1 23- 0(1->(*0 (1->(*- 8 $+(*($ +-& %**- *$ ->($+(;& $* g123- +%-. L$ +$1g$ &%g$(7$ %* 1%/2*$&% % *$ %/31--&$+(;& %& %* ->?g%& 3%1)(% *$ -1)$+(;& % 2& 32%&% 3 2%&% /$*(&/ $*(&- $(+(-&$* %&*$'$- $ -1- 1%/- % $1g(&(&$ @A1g !K. A42? 7%)-/ 3-1 31()%1$ 7%' % -1)$ +-&+1%$ 42% *$ 2&(;& %* %/$- % 1$&/(+(;& @1%/ %&*$+%/ %/ )9/ 3-%&% %& +-)3$1$+(;& +-& %* $/(+- @-/ %&*$+%/. C-& *$ 1$&/%1%&+($ % 31--&%/ $ H(/! 8 *$ 1$&/%1%&+($ 1$&/%1%&+($ % 0(1210(121- @H %* *$+$- $* NAD S% +-)3*%$ *$ 1$&/-1)$+(;& $ 3(127$-. T1$/ *$ $3%121$ %* +%&1- $+(7- 8 *$ +%/(;& %* 3(127$- 8 %* NADH, *$ %&'()$ /% %&+2%&1$ % &2%7- %& *$ 3-/(+(;& (&(+($*, *(/$ 3$1$ *$ /(g2(%&% +$9*(/(/. E* 31-;& 42% 0$ /21g(- %& %* 31-+%/- % %42(*(51(- % *$ 1%$++(;& 1%$++(;& %/$ 31()%1- +-& *$ H(/! H(/! 8 %/32=/ /% (/-+($ (/-+($ +-& *$ 2&(;& % *$ /(g2(%&% /(g2(%&% ! )-*=+2*$ % *$+$-.
Citocro!o o"idasa Facultad de Farmacia y Bioquímica Página 2
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA L$ +$%&$ 1%/3(1$-1($ - % 1$&/3-1% % %*%+1-&%/ %/ 2& /%1(% % +2$1- +-)3*%6-/ 31-%(+-/ 31(&+(3$*%/, $/-+($-/ $ -1-/ +-)3-&%&%/, *-+$*('$-/ %& %* (&%1(-1 % *$ )(-+-&1($. U&$ 31-3(%$ 2&$)%&$* % *-/ +-)3-&%&%/ % *$ +$%&$ 1%/3(1$-1($ %/ 42% +$$ +$$ 2&- % %**%**-// 32%% 32%% /-)% /-)%%1/ %1/%% $ 1%2+ 1%2++( +(;& ;& 8 -> ->( ($+( $+(;& ;& 1%7%1 1%7%1/( /(5* 5*%. %. E/-/ E/-/ +-)3-&%&%/ 2&+(-&$& %& 2& -1%& %%1)(&$- @%/ %+(1 %& +$%&$. E/$ +$%&$ -)$ %*%+1-&%/ %* NADH 8 %* FADH, 8 *- %&1%g$ %& -1)$ /%+2%&+($* $ +$$ +-)3-&%&% %& 2&$ /%1(% % 1%2++(-&%/ 8 ->($+(-&%/ /2+%/(7$/. P-1 <*()-, %* +-)3-&%&% (&$* % *$ +$%&$ % 1$&/3-1% % %*%+1-&%/, +-&-+(- +-)- C-)3*%6- IV - C(-+1-)- ->($/$, %&1%g$ *-/ %*%+1-&%/ @62&- +-& *-/ 31--&%/ $+-)3$$&%/ (-&%/ H $* ->?g%&-, +-& *- 42% /% 31-2+% $g2$. D% %/$ )$&%1$, %* O $+<$ +-)- %* $+%3-1 (&$* % *-/ %*%+1-&%/. E* %%+- &%- %* 2&+(-&$)(%& 2&+(-&$)(%&-- % *$ +$%&$ 1%/3(1$-1($ 1%/3(1$-1($ %/ *$ -)$ % %*%+1-&%/ %*%+1-&%/ % *$/ )-*=+2*$/ % NADH 8 FADH FADH 8 /2 %&1%g$ $* O. E* 3$3%* 42% %/%)3%$ %* O %/ 2&$)%&$*. L-/ +-)3-&%&%/ % *$ +$%&$ % 1$&/3-1% % %*%+1-&%/, (g2$* 42% %* NAD 8 %* FAD, %/9& 31%/%&%/ %& +$&($%/ *()($$/ %& 2&$ +=*2*$ 8, 3-1 *- $&-, &- 32%%& $+2$1 +-)- $+%3-1%/ %1)(&$*%/ % %*%+1-&%/. E& +-&1$/%, %* O %/ $3-1$- $ *$ +=*2*$ %& -1)$ +-&(&2$ 8 %* 31-2+- % %/$ 1%2++(;&, %* $g2$, 32%% *(5%1$1/% $* $)5(%&% 8 $/? 1$&/3-1$1 %*%+1-&%/ 2%1$ % *$ +=*2*$. U&$ 3%1/-&$ $2*$ 31-2+% )9/ % !, *(1-/ % $g2$ 3-1 ?$ %& %* 31-+%/- % %/+$1g$ % %*%+1-&%/ % *$ +=*2*$. L$ +$%&$ 1%/3(1$-1($ &- %/ /-*- 2& )%+$&(/)- % ->($+(;& %* NADH 8 %* FADH. T$)5(=& (%&% 2&$ 3$1(+(3$+(;& 2&$)%&$* %& *$ 1$&/%1%&+($ % %&%1g?$ %/% *-/ %&*$+%/ % *$/ )-*=+2*$/ % *-/ $*()%&-/ 0$+($ %* ATP. E* ->?g%&- )-*%+2*$1 3-/%% )2+0$ )$8-1 $(&($ 3-1 *-/ %*%+1-&%/ 42% *$/ +-%&'()$/ NAD 8 FAD, 8 0$8 g1$& (/)(&2+(;& % *$ %&%1g?$ *(51% 8$ 42% *-/ %*%+1-&%/ -)$-/ %& 2& 31(&+(3(- % *$/ )-*=+2*$/ % $*()%&-/ /-& 1$&/3-1$-/ $ 1$7=/ % *$ +$%&$ 1%/3(1$-1($. U&$ 3$1% +-&/(%1$5*% % %/$ %&%1g?$ %/ +$321$$ %& *-/ %&*$+%/ % ATP ATP.. E* 31-+%/- % -1)$+(;& % ATP ATP $ 3$1(1 % ADP +-& 2(*('$+(;& % *$ %&%1g?$ *(5%1$$ )%($&% %* 1$&/3-1% % %*%+1-&%/ $ 1$7=/ % *$ +$%&$ +$%&$ 1%/3(1$ 1%/3(1$-1 -1($ ($ /% %&%&-)(& )(&$$ -/-1( -/-1(*$+ *$+(;& (;& ->($( ->($(7$. 7$. E& *$ +$%&$ +$%&$ 1%/3(1$ 1%/3(1$-1 -1($ ($ 3$1(+(3$& +2$1- +-)3*%6-/ 31-%(+-/, % *-/ +2$*%/ 1%/ @*-/ +-)3*%6-/ I, III Y IV 3$1(+(3$& %& *$ /?&%/(/ % ATP. TP. C$$ +-)3*%6- 31-2+% 2& ATP $ 3$1(1 % 2& ADP 3-1 +$$ 3$1 % %*%+1-&%/ 1$&/3-1$-. P-1 *- $&-, %& 31(&+(3(-, 3-1 +$$ 3$1 % %*%+1-&%/ 42% $1$7(%/$ *$ +$%&$ 1%/3(1$-1($ %& -$ /2 *-&g(2 %/% %* NADH 0$/$ %* O %/ 3-/(5*% 31-2+(1 1%/ )-*=+2*$/ % ATP. ATP. U& )=-- 1%+2%&% % %>31%/$1 *$ -5%&+(;& % ATP )%($&% *$ -/-1(*$+(-& ->($(7$ %/ +-)- 2&$ 1%*$+(;& PO @P-/$-, O ->(g%&-, 42% /% %(&% +-)- %* &<)%1- % )-*=+2*$/ % ATP -1)$$/ 3-1 +$$ 9-)- % ->(g%&- 1%2+(- $ $g2$. P-1 %6%)3*-, /( /% Facultad de Farmacia y Bioquímica Página 3
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA 1$&/(%1% 2& 3$1 % %*%+1-&%/ % 2&$ )-*=+2*$ % NADH $ 2& 9-)- % O 8 /% -1)$ 1%/ )-*=+2*$/ % ATP ATP,, *$ 1%*$+(;& PO %/ (g2$* $ 1%/.
S#cciato deshidrogeasa L$ /2++(&$- %/0(1-g%&$/$ +$$*('$ *$ %/0(1-g%&$+(;& %/%1-%/3%+?(+$ % /2++(&$- $ 2)$1$-. E/$ %&'()$ %/ (&0(5($ 2%1%)%&% 3-1 )$*-&$-, 2& $&9*-g- %/12+21$* %* /2++(&$- 8 2& %6%)3*- +*9/(+- % 2& (&0(5(-1 +-)3%((7-. C2$&- 1%5/ -1)2*- /2 %-1?$ %* +(+*- %* 9+(- +?1(+-, *$ (&0(5(+(;& % *$ 1%/3(1$+(;& +%*2*$1 3-1 )$*-&$ 31-3-1+(-&- 2&$ % *$/ +*$7%/ % 42% %* /2++(&$- +2)3*% 2& 3$3%* +$$*?(+- %& *$ ->($+(;& % /2/1$-/ 8 42% &- %/ /-*- -1- /2/1$-. L$ /2++(&$- %/0(1-g%&$/$ +-&(%&% 2& g123- 31-/=(+- FAD 42% /% 2&% %& -1)$ +-7$*%&% $ *$ %&'()$ 3-1 )%(- % 2& 1%/(2- H(/. E& g%&%1$*, %* FAD 2&+(-&$ 3$1$ ->($1 5(-42?)(+$)%&% $*+$&-/ @+-)- /2++(&$- $ $*42%&-/ @+-)- 2)$1$-, )(%&1$/ 42% NAD 3$1(+(3$ %& *$ ->($+(;& )9/ %>%1g;&(+$ % $*+-0-*%/ $ $*%0?-/ - +%-&$/. L$ %/0( %/0(1 1-g% -g%&$ &$+( +(;& ;& %* /2++( /2++(&$ &$-- 31-2 31-2+% +% FADH DH, , 42 42%% %5% %5% 1%->( 1%->($ $1/% 1/% $&% $&%// 42 42%% *$ /2++($& /2++($&$$- %/0(1 %/0(1-g%& -g%&$/$ $/$ 32% 32%% % %)31%& %)31%&%1 %1 -1-1- +(+*+(+*- +$$*? +$$*?(+ (+-. -. L$ 1%->( 1%->($+( $+(;& ;& % FADH /% 31-2+% +2$&- /2/ %*%+1-&%/ /% 3$/$& $ *$ +$%&$ 1$&/3-1$-1$ % %*%+1-&%/ )(-+-&1($*. L$ /2++(&$- %/0(1-g%&$/$ %/ *$ <&(+$ %&'()$ %* +(+*- %* 9+(- +?1(+2&($ $ *$ )%)51$&$ @*$/ -1$/ /-& +-)3-&%&%/ % *$ )$1(' )(-+-&1($*, $/? /% 25(+$ 3$1$ %&+$2'$1 %&+$2'$ 1 *-/ %*%+1-&%/ %*%+1-& %/ (1%+$)%&% %&1- % *$ )$42(&$1($ % 1$&/3-1% %*%+1;&(+% *$ )%)51$&$ )(-+-&1($*.
Facultad de Farmacia y Bioquímica Página
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
PROCEDIMIENTO EXPERIMENTAL
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EQUIPOS, EQUIPOS, MATE MATERIALES RIALES Y REACTIVOS REACTIVOS
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
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05! L
05! L
05! L
053 L 05! L
053 L 05! L
24 RESULTADOS M%)a& + ag&%ga& 1 L .% a%i
%$/4 Seg#i!ieto Seg#i!ieto de )a reacci6 reacci6 cata)i+ cata)i+ada ada ,or )a )actato )actato deshidroge deshidrogeasa4 asa4 S% -527- *-/ /(g2(%&%/ 1%/2*$-/: -
T#$os N= / % -: S% -5/%17$ 2& )(/)- +-*-1 @7%1-/- +-& (%1%&%/ (&%&/($%/ @%* 25- N %/ )9/ (&%&/- 42% %* N !. E/- %/ %5(- $ 42% %& %* 25- N ! 0$8 )$8-1 +$&($ % /25/1$-, 3-1 *- $&- *$ *$+$- %/0(1-g%&$/$ 31-2+(19 )$8-1 +$&($ % P(127$-, *- +2$* -+$/(-&$19 42% %& %* 25- N ! 0$8$ 2&$ )$8-1 +-&+%&1$+(;& % NADH, $%)9/ % *-/ 8$ 31%/%&% %& %* 0-)-g%&('$-, *2%g%/-/ -&$1$& /2/ %*%+1-&%/ $ *$ +$%&$ 1$&/3-1$-1$ % %*%+1-&%/ -1)$&- 2&$ )$8-1 +$&($ % $'2* % )%(*%&- 1%2+(-.
Facultad de Farmacia y Bioquímica Página
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA -
T#$o N= ? % 5: S% -5/%17$ 2& )(/)- +-*-1 @$'2*$- +-& (%1%&%/ (&%&/($%/ @%* 25- N %/ )9/ (&%&/- 42% %* N , E/- %5(- $ 42% %& %* 25- N ! 0$8 )$8-1 +$&($ % /25/1$-, 3-1 *- $&- /2+%%19 *- )(/)- 42% %& *-/ 25-/ ! 8 , +-& *$ (%1%&+($ 42% %& %/-/ 25-/ /% (%&% 0-)-g%&('$- % +-1$';&, 8 %/% (%&% )%&-1 +-&+%&1$+(;& % L$+$- %/0(1-g%&$/$ 8 NADH.
i *&a NF
Fig#ra N= /: S% )2%/1$ *-/ 1%/2*$-/ %* S%g2()(%&- % *$ 1%$++(;& +$$*('$$ 3-1 *$ *$+$- %/0(1-g%&$/$, +$$ 25- +-&(%&% (%1%&%/ 1%$+(7-/: ! L$+$- % /-(- K.! M, /-*. % $'2* % )%(*%&- 8 0-)-g%&('$- % 0?g$-. /-*. % $'2* % )%(*%&- 8 0-)0-)-g% g%&( &('$ '$- % 0?g$ 0?g$-.. L$+ L$+$ $-- % /-( /-(-- K.! K.! M, /-*. /-*. % $'2* $'2* % )%( )%(*% *%&&- 8 0-)-g%&('$- % +-1$';&. /-*. % $'2* % )%(*%&- 8 0-)-g%&('$- % +-1$';&. -4 Seg#i!ieto Seg#i!ieto de de )a reacci6 reacci6 cata)i+ada cata)i+ada ,or )a )a citocro!o citocro!o o"idasa S% -5/%17$& *-/ /(g2(%&%/ 1%/2*$-/: 1%$++(;&, $& /-*- /% )2%/1$ %* +-*-1 +-*-1 (&(+($* % *$ T#$o N= /: S% -&/%17$ 42% &- 0$8 1%$++(;&, /-*2+(;& % P %&(*%&($)(&$ $ 2& 3H % ., 42% 7%&1?$ $ /%1 *$ -1)$ 1%2+($. - T#$o N=-: S% -5/%17$ %* +$)5(- % *$ -1)$ 1%2+($ $ 2&$ -1)$ ->($$ @)$11;& 3-1 $++(;& %* C(-+1-)- C ->($-. /% 31-2+% 2&$ (&0(5(+(;& % - T#$o N= ?: S% -5/%17$ 42% &- 0$8 1%$++(;&, %5(- $ 42% /% 31-2+% *$ +$%&$ % 1$&/3-1% %*%+1;&(+- )(-+-&1($* @3-1 %* +($&21- $ &(7%* %* +(-+1-)->($/$. -
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA -
)$11;&. E/- %/ %5(- $ 42% %& %* 0-)-g%&('$0-)-g%&('$- &T#$o N= 5: S% -5/%17$ 2& +-*-1 )$11;&. /-*- %/9 31%/%&% *$ +$%&$ 1$&/3-1$-1$, $)5(=& %>(/%& NADH, *$+$- 8 %&'()$/ *$+$- %/0(1-g%&$/$. E/- -+$/(-&$1$ 42% %* 1$&/3-1% % %*%+1-&%/ /% 31-2'+$ 3-1 %* +-)3*%6- NADH %/0(1-g%&$/$ 8 &- 3-1 %* +-)3*%6- /2++(&$- %/0(1-g%&$/$, 8$ 42% %/9 %/9(&0(5($ 3-1 %* )$*-&$-.
T25- N
T25- N
T25- N
T25- N
ig*&a NF
Fig#r Fig#raa N= -: /% )2%/1$ *-/ 1%/2*$-/ %* S%g2()(%&- % *$ 1%$++(;& +$$*('$$ 3-1 *$ +(-+1-)- ->($/$, +$$ 25- +-& (%1%&%/ 1%$+(7-/: ! S-*. 3%&(*%&($)(&$ 8 52%1 -/$- K.! M 3H .. H-)-g%&('$- % +-1$';&, S-*. 3%&(*%&($)(&$ 8 52%1 -/$K.! K.! M 3H .. .. HH-))-g% g%&( &('$ '$- % +-1$ +-1$'; ';&, &, +($& +($&21 21-- % /-( /-(-- K.! K.! M 8 S-*. S-*. 3 %&(* %&(*%& %&( ($) $)(& (&$. $. HH-))-g% g%&( &('$ '$- % +-1$' +-1$';&, ;&, )$*)$*-&$ &$-- % /-( /-(-- K.! K.! M 8 S-*. S-*. 3 %&(*%&($)(&$. ?4 Seg#i!ieto Seg#i!ieto de )a reacci6 reacci6 cata)i+ cata)i+ada ada ,or )a s#cciato s#cciato deshidrog deshidrogeasa easa 1$&/3-1% % %*%+1-&%/ %*%+1-&%/ 3-1 T#$o N= /: S% -5/%17$ 2& +-*-1 &$1$&6$, %/- %5(- $ %* 1$&/3-1% )%(- %* +-)3*%6- II % )$&%1$ &-1)$* /(& 31%/%&+($ % &(&g<& (&0(5(-1. - T#$oN= $oN= -: /% -5/%17$ 2& +-*-1 *(g%1$)%&% &$1$&6$, %/- %5(- $ 42% /% 2(*('; )$*-&$- % /-(-, @(&0(5(-1 +-)3%((7- %* /2++(&$- %/0(1-g%&$/$, 3%1- +-) 3-%)-/ -5/%17$1 0$8 2&$ 1%2++(;& %* $'2* % )%(*%&-, %5(- $ 42% %& %* 0-)-g%&('$- &- /-*- %/9 31%/%&% *$ +$%&$ 1$&/3-1$-1$, $)5(=& %>(/%& NADH, *$+$- 8 %&'()$/ *$+$- %/0(1-g%&$/$. -
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA -
-
T#$o N= ?: S% -5/%17$ 2&$ +-*-1$+(;& 7%1-/$, %/- %5(5- $ 42% &- /% 2(*('; %* /2/1$- @/2++(&$-. (%1%&+($ % (&%&/($, %/- %/ %5(- 42% %* 25- T#$os N= 5 % ': /% -5/%17$ 2&$ (%1%&+($ **%7$ /25/1$- S2++(&$- % /-(-, *- +2$* $2)%&$1$ *$ +-&+%&1$+(;& % A'2* % )%(*%&- 1%2+(-, $ (%1%&+($ %* 25- . A)5-/ 25-/ +-&(%&% +-&(%&% +($&21- @(&0(5(-1. (%1%&+($ % (&%&/($, %/- %/ %5(- 42% %* 25- T#$os N= @ % 9: /% -5/%17$ 2&$ (%1%&+($ **%7$ /25/1$- S2++(&$- % /-(-, *- +2$* $2)%&$1$ *$ +-&+%&1$+(;& % F%11-+($&21-, $ (%1%&+($ %* 25- , -&% 0$519 2&$ )%&-1 +-&+%&1$+(;& % %11-+($&21-.
i *&a
Fig# Fig#ra ra N= ?: S% )2%/1$& *-/ 1%/2*$-/ -5%&(-/ %& %* /%g2()(%&- % *$ 1%$++(;& +$$*('$$ +$$*('$$ 3-1 *$ /2++(&$- %/0(1-g%&$/$, %/0(1-g%&$/$, +$$ 25- +-& (%1%&%/ (%1%&%/ 1%$+(7-/. ! A'2* % )%(*%&-, 52%1 -/$- K.! M, S2++(&$- % /-(- K.! M 8 0-)-g%&('$- % 0?g$-. A'2* % )%(*%&-, 52%1 -/$- K.! M, S2++(&$- % /-(- K.! M, M$*-&$- % /-(- 8 0-)-g%&('$- % 0?g$-. A'2* % )%(*%&-, 52%1 -/$- K.! M, $g2$ 5(%/(*$$, M$*-&$- % /-(- 8 0-)-g%&('$- % 0?g$-. A'2* % )%(*%&-, 52%1 -/$- K.! M, S2++(&$- % /-(- K.! M, +($&21- % /-(- K.! M 8 0-)-g%&('$- % 0?g$-. A'2* % )%(*%&-, )%(*%&-, 52%1 -/$- K.! M, $g2$ 5(%/(*$$ 5(%/(*$$,, +($&21+($&21- % /-(- K.! M 8 0-)-g%&('$% 0?g$ 0?g$-. -. A'2* '2* % )%( )%(*% *%&-, &-, 52% 52%11 -/$ -/$- K.! M, S2++( S2++(&$ &$-- % /-( /-(-- K.! K.! M, %11-+($&21- % 3-$/(- K.! M 8 0-)-g%&('$- % 0?g$-. A'2* % )%(*%&-, 52%1 -/$- K.! M, $g2$ 5(%/(*$$, %11-+($&21- % 3-$/(- K.! M 8 0-)-g%&('$- % 0?g$-.
2I4 DISCUSIONES
Facultad de Farmacia y Bioquímica Página 12
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA E& %* /%g2()(%&- % *$ 1%$++(;& +$$*('$$ 3-1 %* *$+$- %/0(1-g%&$/$, /% 2(*('- $'2* % )%(*%&-, %* +2$* $+2$1$ +-)- 2& $+%3-1 % %*%+1-&%/, 3$/$&- % 2&$ -1)$ ->($$ @$'2* $ 2&$ 1%2+($ @(&+-*-1-.
S% 2(* 2(*(' ('-- $)5 $)5(= (=&& %* *$+ *$+$ $-- % /-(/-(-,, %* +2$* +2$* $+2$ $+2$1$ 1$ +-)+-)- /25/ /25/1$ 1$-- %* %* *$+ *$+$ $- %/0(1-g%&$/$, %/0(1-g%&$/$, $+2$&$+2$&- +-& /2 +-$+-1 +-$+-1 NAD , 42% /% 1%2+(19 1%2+(19 $ NADH, NADH, 8 %* 31-2+31-2+/%19 P(127$-. E* NADH *2%g- 3$/$1$ $ *$ +$%&$ 1$&/3-1$-1$ % %*%+1-&%/ 3$1$ -&$1 /2/ %*%+1-&%/.
D%5(D%5( - $ /2 3-% 3-%&+( &+($$ % 1%2+ 1%2++( +(;& ;& @E- @E- K.K! K.K! 7-*( 7-*(-/ -/,, %* $'2* $'2* % )%( )%(*% *%&&- $+2$1 $+2$1$$ $+%3$&$+%3$&- *-/ %*%+1-&%/ %*%+1-&%/ *2%g- % *$ 1%2++(;& %* FMN $ FMNH @E- K.K 7-*(-/ 7-*(-/ %& %* +-)3*%6+-)3*%6- NADH %/0( %/0(1-g% 1-g%&$/$ &$/$,, $&%/ $&%/ 42% %/-/ 3$/%& $* C( 5 ->($ @E- K.! K.! ->($-- @E- 7-*(-/ %* +-)3*%6- +(-+1-)- 5+!. R%+-1%)-/ 42% %* 1$&/3-1% % %*%+1-&%/ /% $19 % -1)$ +1%+(%&%/ $ /2/ 3-%&+($*%/ % 1%2++(;&. E& %* /%g2()(%&/%g2()(%&- % *$ 1%$++(;& 1%$++(;& +$$*('$$ 3-1 %* *$+$- %/0(1-g%&$/$, %/0(1-g%&$/$, %& *-/ 25-/ ! 8 , /% -5/%17$ 2& )(/)- +-*-1 +-& (%1%&%/ (&%&/($% (&%&/($%/. /. E/- %/ %5(- $ 42% %& 2& 250$8 /25/1$- 8 %& %* -1- &-, %/ %+(1 *$ *$+$- %/0(1-g%&$/$ 31-2+(19 )$8-1 +$&($ % P(127$-, %/- -+$/(-&$1$ 42% %& %* 25- ! 0$8$ 2&$ )$8-1 +-&+%&1$+(;& % NADH, $%)9/ % *-/ 8$ 31%/%&% %& %* 0-)-g%&('$-, *2%g- %/-/ -&$1$& /2/ %*%+1-&%/ $ *$ +$%&$ 1$&/3-1$-1$ % %*%+1-&%/ -1)$&- 2&$ )$8-1 +$&($ % $'2* % )%(*%& 1%2+(-. . L- )(/)- 3$/$1$ +-& *-/ 25-/ 8 . T$)5(=& /% -5/%17$ 42% *-/ 25-/ ! 8 /-& % (%1%&% +-*-1 42% *-/ 25-/ 8 . E/- %/ %5(- $ 42% *-/ 25-/ /-& % (%1%&%/ %6(-/. L-/ 25-/ ! 8 /-& %* 0-)-g%&('$- % 0?g$- 8 *-/ 25-/ 8 /-& %* 0-)-g%&('$% +-1$';&. L$ %>3*(+$+(;& 5(-42?)(+$ /%1($ 42% %& %* 0?g$- /% 31-2+% %* K % *$ g*2+-&%-g=&%/(/, %/ %+(1 0$519 2&$ )$8-1 +-&+%&1$+(;& % L$+$- %/0(1-g%&$/$ 8 NADH. E/ 3-1 %/$ %/$ 1$';& 1$';& 42% %& *-/ 25-/ % 0-)-g% 0-)-g%&(' &('$$- % +-1$';& +-1$';& 31%/%&$ 31%/%&$1$ 1$ 2& Facultad de Farmacia y Bioquímica Página 13
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA +-*-1 $'2*, 8$ 42% 0$8 2&$ )$8-1 +-&+%&1$+(;& % A'2* % M%(*%&- ->($-, $ (%1%&+($ %* 0?g$- 42% )2%/1$ 2& +-*-1 $'2* 7%1-/-, %5(- 42% 0$8 )$8-1 +$&($ % A'2* % )%(*%&- 1%2+(- @(&+-*-1-. E& *$ 312%5$ /(g2(%&% /% 2(*('- S-*. PF%&(*%&($)(&$ 42% /% 2(*('$ 3$1$ *$ 312%5$ % ->($/$ *$ +2$* /% 1%$*('$ %& 5$+%1($/. P$1$ %%+- % *$ 319+(+$, *- 2(*('$1%)-/ 3$1$ %* /%g2( /%g2()( )(%& %&-- % *$ 1%$++ 1%$++(; (;&& +$$* +$$*(' ('$$ $$ %* +(+(-+1 +1-) -)-- -> ->( ($/$ $/$.. L-/ L-/ +-*+-*-1$ 1$& &%/ %/ % 3 %&(*%& %&(*%&($) ($)(&$ (&$ /-& $)(&$/ $)(&$/ $1-)9( $1-)9(+$/ +$/ 31()$1 31()$1($/ ($/,, %1(7$%1(7$-// ($)(& ($)(&-- %* 5%& 5%&+%&+%&-.. L$ +(-+1-)- ->($/$ &- 1%$++(-&$ (1%+$)%&% +-& %* 1%$+(7- 3%&(*%&($)(&$ 3%1- ->($ $* +(-+1-)- +, %* 42% $ /2 7%' ->($ %* 1%$+(7-.
L$ 1%$++(;& /% 32%% -5/%17$1 -5/%17$1 %& *-/ 25-/ ! 8 . E& %* 25- ! &- 0$8 1%$++(;&, $& /-*- /% )2%/1$ %* +-*-1 (&(+($* % *$ /-*2+(;& % P %&(*%&($)(&$ $ 2& 3H % ., 42% 7%&1?$ $ /%1 *$ -1)$ 1%2+($. E& %* 25- , /% -5/%17$ %* +$)5(- % *$ -1)$ 1%2+($ $ 2&$ -1)$ ->($$ @)$11;& 3-1 $++(;& %* C(-+1-)- + ->($-. L2%g- $)5(=& /% 2(*('- %* +($&21- % 3-$/(-, 42% 31-2+% 2&$ (&0(5(+(;& % *$ +$%&$ % 1$&/3-1% %*%+1;&(+- )(-+-&1($* $ &(7%* %* +(-+1-)- ->($/$. E* +($&21- %/ 2&- % *-/ 7%&%&-/ )9/ ;>(+-/ 8 % $++(;& )$/ 193($ 42% /% +-&-+%. E* +($&21- /% 2&% $* F% % *$ +( $ % ()3(% 42% -&% /2/ %*%+1-&%/ $* O.
Facultad de Farmacia y Bioquímica Página 1
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
E& %* 25- &- /% -5/%17$ %* +-*-1 )$11;&, *- +2$* (&(+$1?$ 42% *$ 1%$++(;& &- /% 0$ +-)3*%$-. E/- %/ %5(- $ 42% /% 2(*('- +($&21-, 42% +-)- 8$ )%&+(-&$)-/ ()3(% *$ **%g$$ % *-/ %*%+1-&%/ $* ->(g%&-. L2%g- $)5(=& /% 2(*('- )$*-&$- % /-(-, 42% %/ 2& (&0(5(-1 +-)3%((7- 42% /% 2&% $* /((- $+(7- % /2++(&$- %/0(1-g%&$/$ %& %* +(+*- %* $+(- +?1(+-, +-)3((%&- +-& %* /2++(&$- %5(- $* 3$1%+(- % /2/ %/12+21$/.!K
E/- $%+$1?$ $ *$ +$%&$ 1$&/3-1$-1$ % %*%+1-&%/, /(& 42% *$ 1%$++(;& +-& %* 3 %&(*%&($)(&$ /% 31-2'+$, /-*- /( %/ 42% 1$5$691$)-/ %& 319+(+$ % -1)$ $(/*$$, 3%1 3-%)-/ -5/%17$1 %& %* 25- +-)+- )- /% 31-2+% *$ 1%$++(;&, -5/%179&-/% -5/%179& -/% 2& +-*-1 )$11;&. E/- %/ %5(- $ 42% %& %* 0-)-g%&('$- &- /-*- %/$ 31%/%&% *$ +$%&$ 1$&/3-1$-1$, $)5(=& %>(/%& NADH, *$+$- 8 %&'()$/ *$+$- %/0(1-g%&$/$. E/- -+$/(-&$1$ 42% %* 1$&/3-1% % %*%+1-&%/ /% % 3-1 %* +-)3*%6- NADH %/0(1-g%&$/$ 8 &- 3-1 %* +-)3*%6/2++(&$- %/0(1-g%&$/$. Facultad de Farmacia y Bioquímica Página 1!
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA E& %* /%g2()(%&- % *$ 1%$++(;& +$$*('$$ 3-1 *$ /2++(&$- %/0(1-g%&$/$, /% 32%% -5/%17$1 %& %* 25- !, %* 1$&/3-1% % %*%+1-&%/ 3-1 )%(- %* +-)3*%6- II % )$&%1$ &-1)$* /(& 31%/%&+($ % &(&g<& (&0(5(-1. L2%g- /% 2(*('- )$*-&$- % /-(- %& *-/ 25-/ 8 , +-)- 8$ )%&+(-&$)-/ %* )$*-&$- $+<$ (&0(5(%&- +-)3%((7$)%&% $* /2++(&$%/0(1-g%&$/$!K, 3%1- +-)- 3-%)-/ -5/%17$1 0$8 2&$ 1%2++(;& %* $'2* % )%(*%&@(&+ @(&+-* -*-1-1- %5( %5(- $ 42% 42% &- 1$5 1$5$6 $6$) $)-/ -/ %& 31$+ 31$+( (+$ +$ % -1) -1)$$ $(/* $(/*$$ $$,, 8$ 42% %& %* 0-)-g%&('$- &- /-*- %/$ 31%/%&% *$ +$%&$ 1$&/3-1$-1$, $)5(=& %>(/%& NADH, *$+$- 8 %&'()$/ *$+$- %/0(1-g%&$/$. E/- -+$/(-&$1$ 42% %* 1$&/3-1% % %*%+1-&%/ /% % 3-1 %* +-)3*%6- NADH %/0(1-g%&$/$ 8 &- 3-1 %* +-)3*%6- /2++(&$- %/0(1-g%&$/$. E& *-/ 25-/ 8 , 2(*('$)-/ +($&21- % 3-$/(-, 42% +-)- )%&+(-&$)-/ $&%1(-1)%&% 31-2+% 2&$ (&0(5(+(;& % *$ +$%&$ % 1$&/3-1% %*%+1;&(+- )(-+-&1($* $ &(7%* %* +(-+1-)+(-+1-)- ->($/$. ->($/$. E/- &- $%+$1$ *$ **%g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
D%5(- $ /2 3-%&+($ % 1%2++(;& @E- K. 7-*(-/, %* F%11(+($&21- $+2$1$ $+%3$&*-/ %*%+1-&%/ *2%g- % *$ 1%2++(;& %* C( $ ->($- $ C( $ 1%2+(- @E- K. 7-*(-/ %& %* +-)3*% +-)3*%66- +(-+1-)+(-+1-)- ->($/$ ->($/$,, $&%/ $&%/ 42% %/-/ %/-/ 3$/%& $* ->(g%& ->(g%&-- @E- K.! K.! 7-*(-/. 7-*(-/. R%+-1%)-/ 42% %* 1$&/3-1% % %*%+1-&%/ /% $19 % -1)$ +1%+(%&%/ $ /2/ 3-%&+($*%/ % 1%2++(;&. E/- *- 3-%)-/ -5/%17$1 %& %* 25- 8 , -&% $)5(=& /% -5/%17$ 2&$ (%1%&+($ % (&%&/($, %/- %/ %5(- 42% %* 25- **%7$ /25/1$- S2++(&$- % /-(-, *+2$* $2)%&$1$ $2)%&$1$ *$ +-&+%&1$+(;& +-&+%&1$+(;& % F%11-+($&21-, F%11-+($&21-, $ (%1%&+($ (%1%&+($ %* 25- , -&% 0$519 2&$ )%&-1 +-&+%&1$+(;& % %11-+($&21-.
2II4 CONCLUSIONES Facultad de Farmacia y Bioquímica Página 1"
PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA S% (%&((+- *$ $+(7($ % *$ %&'()$ *$+$- %/0(1-g%&$/$ %& (%1%&%/ %6(-/, /(%&- )$8-1 %& %* 0?g$-. S% (%&((+- *$ 31%/%&+($ % +(-+1-)- ->($/$ +-& %* 3 %&(*%&($)(&$ 8 %* %%+- % /2/ (&0(5(-1%/ +-)- %* (-& +($&21-, $+2$&- $* &(7%* % +(-+1-)$. S% (%&((+- *$ $+(7($ % *$ %&'()$ /2++?&(+$ %/0(1-g%&$/$ )%($&%/ %* 2/- % $+%3-1%/ $+%3-1%/ %*%+1;&(+-/ %*%+1;&(+-/ +-*-1%$-/ +-)- %* $'2* % )%(*%&-, )%(*%&-, $+2$&- $* &(7%* % *$/ *$7(&$/ %* +-)3*%6- NADH %/0(1-g%&$/$. S% (%&( (%&((+ (+-- %* %%+%%+- (&0(5( (&0(5(-1 -1 % $*g2&$ $*g2&$// /2/$&+( /2/$&+($/ $/ +-)+-)- %* )$*-&$ )$*-&$-, -, $+2$&- $ &(7%* %* +-)3*%6- /2++?&(+- %/0(1-g%&$/$.
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA 2III4 CUESTIONARIO
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-4 Usos c)>icos de )a ea)#aci6 de )as e+i!as est#diadas P$1$ *$ *$+$- %/0(1-g%&$/$ 42% %/ 2&$ %&'()$ 42% /% %&+2%&1$ %&1- % *$/ +=*2*$/, /2 $3$1(+( $3$1(+(;& ;& %& *$ /$&g1% (&(+$ (&(+$ 2& 31-+%/31-+%/- % %/12++( %/12++(;& ;& +%*2*$1, +%*2*$1, %/$ %/$ -+$/(-& -+$/(-&$$ $$ 3-1 (&%++(-&%/, 1$2)$/ - &%-3*$/($/. N(7%*%/ %*%7$-/ % %/$ %&'()$ 32%%& (&(+$1 (&$1$g2- $* )(-+$1(-, %&%1)%$%/ 0%)$-*;g(+$/, 0%3$-3$?$/, )%9/$/(/, %&1% -1-/. E& %* +$/- % *$ +(-+1-)- ->($/$ /2 %7$*2$+(;& %& 31-+%/-/ +*?&(+-/ %/ )9/ 42% -- $&% 2&$ (&(&->( >(+$+ +$+(; (;&& 3-1 +($& +($&2121-/, /, 42% 42% /% ($g ($g&&-/ /(+ (+$$ 9+(* 9+(*)% )%& &%% $&%/ $&%/ *-/ *-/ /?& /?&-) -)$/ $/ +$1$+%1?/(+-/ % %/% (3- % (&->(+$+(-&%/. L$ $3$1(+(;& %& *$ -1(&$ % &(7%*%/ %>+%/(7-/ % /2++(&$- %/0(1-g%&$/$ %& +-&62&- +-&, $*$ +%-g*2$1$-, +(1$- 8 )$*$-# $/? +-)- &(7%*%/ $&-1)$*%/ % 2)$1$- (&(+$& 2& 1$/-1&- +-&-+(- +-)- %(+(%&+($ % 2)$1$/$.
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I4 REFERENCIAS BIBLIOGRHFICAS !. MW**%1 X. B(-42?)(+$: F2&$)%&-/ 3$1$ M%(+(&$ 8 C(%&+($/ % *$ V($. E(-1($* R%7%1= S.A. $ E(+(;&. B$1+%*-&$, KK. P9g. !! . H(** R 8 X8/% G. F(/(-*-g?$. E(-1($* M%(+$ P$&$)%1(+$&$ S.A. 1$ E(+(;&. M$1(, KK. P9g. !! . V-% V-% D, VV-% J 8 P1$ C0. F2&$)%&-/ % B(-42?)(+$. E(-1($* M%(+$ P$&$)%1(+$&$ $ S.A. E(+(;&. M$1(, KK.P9g. K!
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA . A)%1(+$& C0%)(+$* S-+(%8. Q2?)(+$, 2& 31-8%+- % *$ ACS. !1$ E(+(;&. E(-1($* R%7%1%. M$1(, KK. P$g. !. . T0-)$/ M. D%*7(&. B(-42?)(+$, *(51- % %>- +-& $3*(+$+(-&%/ +*?&(+$/. $ E(+(;&. E(-1($* R%7%1%. B$1+%*-&$, KK. P$g. K. . J-0& X(*%8 S-&/. B(-42?)(+$. 1$ E(+(;&. E(-1($* M=(+$ P$&$)%1(+$&$. B2%&-/ A(1%/, KK. P$g. . . C$)3 C$)35% 5%** ** M$18 M$18 ., ., F$11% F$11%** ** S0$ S0$ O. Bioquímica. $ E(+(; E(+(;&. &. E(-1 E(-1($* ($* T0-)/T0-)/-&& I&%1&$(-&$*. M=>(+- D.F., KK. P$g. K!. . M$+F$(&. P12%5$/ 5(-42?)(+$ 3$1$ *$ (%&((+$+(;& % 5$+%1($/ % ()3-1$&+($ +*?&(+$. 1$ E(+(;&. E(-1($* M=(+$ P$&$)%1(+$&$. B2%&- A(1%/, KK. P$g. . . G$11(- A. T%(6-& J. F2&$)%&- % B(-42?)(+$ E/12+21$*. $ E(+(;&. E(-1($* T%5$1. T%5$1. M$1(, KK. P$g. !. !K. N-1)$ N-1)$ C., R$$%* R$$%* M. P1-5*%) P1-5*%)$$ 5(-42?) 5(-42?)(+(+- 5(-%&% 5(-%&%1g= 1g=(+ (+$. $. R%7(/ R%7(/$$ % %2+$+( %2+$+(;& ;& 5(-42?)(+$ @M=>(+-. KK# !@,: !K!K. !!. F1$&[ X$*/0. U& 31()%1 +21/- % (&g%&(%1?$ %*%+1-42?)(+$. !1$ E(+(;&. E(-1($* C*25 U&(7%1/($1(-. B$1+%*-&$, KK. P$g. !. !. X%1&%1 M. B(-42?)(+$. F2&$)%&-/ 3$1$ *$ 7($ 8 +(%&+($/ % *$ 7($. \ %. B$1+%*-&$, E/3$$: E(-1($* R%7%1%# KK. P$g/: " . ! . L$+$%/0(1-g%&$/$ @ %& *?&%$. D(/3-&(5*% 03:%/.([(3%($.-1g([(L$+$-]%/0(1-g%&$/$. C-&/2*$-: K F%5 K!.
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PRESENCIA DE LACTATO DESHIDROGENASA, CITOCROMO OXIDASA OXIDASA Y SUCCÍNICO DESHIDROGENASA EN TEJIDOS DE RATA
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