INGENIERIA QUIMICA Y BIOQUIMICADescripción completa
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aaFull description
HPLC, KCKT, prosedur, sampel preparation
hplc
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metodos instrumentalles
laptapFull description
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Instituto Politécnico Nacional Unidad Profesional Interdisciplinaria de Biotecnología Métodos cuantitativos
Cromatogra Croma tografía fía Líquida de Alta A lta Resolución (HPLC) Partes del equipo
Bernal Castañeda Bianca Edith Domínguez Gómez Daniel Enríquez Cerda Noemí A. Guevara García Amaris Abril 6, 2010
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Equipo para Cromatografía Cromatografía Líquida de Alta Resolución (HPLC)
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Depósitos para la fase movil Recipientes inhertes que suelen ser botellas de vidrio y tubos de teflón provistos de aditamentos indispensables para eliminar los gases disueltos y partículas que pudiera tener la fase movil
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Tipos de elución Arrastrar mediante un Elución: Arrastrar
líquido (o fluido) o solvente sustancias adheridas o absorbidas
Elución isocrática: Separación con disolvente de composición constante Elución con gradiente: Se utilizan dos o tres disolventes de distinta polaridad y se varía la composición de forma continua o mediante etapas escalonadas.
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Eluyent Eluy entes es típi típicos cos
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Sistemas de bombeo Son nece necessarios rios para ara cons onsegui eguirr y regu regullar la presi esión y veloc elociidad de fluj flujo o de la fase movi ovil. Cuando la fase ase movi ovil es una una mez mezcla de solv olvent entes, la bomba mba se progr ograma par para tomar omar los los dif diferen erente tess solv solven ente tess en una una prop propor orci ción ón dete determ rmin inad ada a Características Generar presiones por encima de 6000 psi Libre Libre de pulsaciones pulsaciones Intervalo Intervalo de caudales caudales de 0.1 a 10 mL/min. Reprodu Reproducibl cible e Resistent Resistente e a la corrosión corrosión Tipos: Bombas reciprocas
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Sistemas de inyección Son válvulas dosificadoras que permiten regular la aplicación de la muestra en volumenes de 5 a 500 l.
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Columna Contiene a las partículas de la fase estacionaria
Columnas analí ti ticas Acero
inoxidable (hasta 10000 psi)
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Tipos de rellenos de la columna Pelic Pelicul ular ar Bolas de vidrio o polímero, no porosas y esféricas Sobre su superficie se deposita una capa delgada y porosa de sílice, alúmina o una resina de intercambio iónico
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Columnas de sílice
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Detectores Responderán a una propiedad de la fase móvil Características deseadas Sensibilidad
adecuada Estabilidad y reproducibilidad buenas
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Detectores de índice de refra refracción cción
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