Laporan Resmi Analisis Instrumen

LAPORAN RESMI ANALISIS INSTRUMEN KROMATOGRAFI GAS II

 Nama kelompok  1. LUTFI PRESTIKASARI

( 16130981B )

2. NUR AFIA

( 16130993B )

3. LESTARI

( 1613099!B )

D III FARMASI FAKULTAS FARMASI UNIVERSITAS SETIA BUDI SURAKARTA 2015

I.

TU"UAN U#$%k me#&e$a'% pe#&a%' $empea$% kolom $e'a*ap +ak$% e$e#, ($R)

II.

-ASAR TERI Ala,a# koma$o&a/ &a, ,ea&a #,$%me# %#$%k a#al,, /,ko kma me#*%*%k po,, $epe#$#& *a# a#ak *paka a*ala' ak$% a#al,, ela$/ ,#&ka$ • Sampel a#& *pel%ka# *alam ,kala 1410 5l,ekal #7ek, • Kolom *apa$ *paka e%la#& • Ala# /a,e &eak $eko#$ol *a# keepa$a# $e$ap • Sa#&a$ m%*a# $e7a* pe#amp%a# %ap ,ampel ke*alam ala# /a,e • • • •

&eak  Pem,a'a# /,k $e7a* * *alam kolom Ba#ak maam *e$ek$o a#& *apa$ *paka Sa#&a$ m%*a' *&a%#&ka# *e#&a# ala$ la# ,epe$ SFT4IR 

-aa p,a' koma$o&a/ &a, *$e#$%ka# 2 /ak$o a$% a. E/,e#, kolom  e#e#$%ka# peleaa# p%#ak koma$o&am  -apa$ *%k% *a N *a# :ETP  Beka$a# *e#&a# lama#a +ak$% kompo#e# a#& *a#al,,  ea*a *alam kolom  . E/,e#, pela%$  e#e#$%ka# po,, p%#ak (+ak$% e$e#, e$ama') I#,$%me#$a, *a koma$o&a/ &a, a#& $epe#$#& a*ala' 1. -epo &a, pema+a Saa$  lemam *a ,$%k$% kma kem%#a# $#&& ala# &a, pema+a 'a%, $e$ap ,elama opea,o#al *a# la7% ala# &a, *a$% ole' pe#&a$% $eka#a#. Teka#a# ala# &a, pema+a *,e,%aka# *e#&a# ko#*, ke%$%'a# a#al,, a,a#a 104;0 p, *e#&a# la7% ala# 2;41;0 mlme#$. a#& $epe#$#& *a &ea#& ,%#$k a*ala' po&am $empea$% pa*a &ea#& ,%#$k. Um%m#a $empea$% *a$% ,ampa ;0 0? * a$a, $$k **' kompo#e# a#& *a#al,,. 3. Kolom koma$o&a/ Kolom me%paka# 7a#$%#& koma$o&a/ kee#a pem,a'a# kompo#e# $e7a* *kolom. aam kolom a*a *%a maam a. Kolom $epak#&

 . Kolom $e%kakaple  !. Ko#$ol $empea$%  aam $empea$% * *alam o@e# a$%  I,o$'emal $empea$% *a$% $e$ap ,elama a#al,,  Pemo&ama# $empea$% $empea$% *a$% #ak ,eaa $ea$% ,elama e#$a#& +ak$% a#ala,, o#$o'#a 30 0 III.

ALAT -AN BA:AN a. Ala$ 1. Koma$o&a/ &a, S'ma*%
I=.

?ARA KER"A 1. e#&a#$% $empea$% kolom 1800? #7ek$o 190 0? *a# *e$ek$o 2000? *$%#&&% ,ampa *peole' Ca,e l#eD a#& ak  2. e#%#$k ; 5l la%$a# me#$ol pa*a K< me#&'$%#& 'a&a N *a# :ETP 3. e#%#$k ; 5l la%$a# kam/e pa*a K< me#&'$%#& 'a&a N *a# :ETP !. e#%#$k ; 5l la%$a# me#$ol *a# kam/e pa*a K< me#&'$%#& 'a&a e,ol%, ;. Tempea$% kolom *$%%#ka# me#7a* 1;00? K< *aka# ,ampa *peole' Ca,e l#eD a#& ak  6. e#&%la#& po,e*% 2 ,* ; *pl' ko#*, a#& memeka# 'a&a e,ol%, a#& ak  . e#e#$%ka# ko#*, a#al,, a#& ak 

=.

:ASIL 1. Data

S%'% kolom

 1;0 0?

S%'% #7e$o

 180 0?

S%'% *e$e$o

 190 0?

Keepa$a# ala# &a, :2

 0

Keepa$a# ala# %*aa

 0

Keepa$a# ae

 1;0 mlme#$

Kondisi S! Ko"o# 0 1!0  ? e#$ol • •

Kam/e 

•

amp%a#

N

$ETP

T%

R

28118 2!881 4

0001 00080 4

12 1 4

4 4 13!;3

3283; 222! 4

00061 00089 4

13 13 4

4 4

0

1;0  ? •

e#$ol

•

Kam/e 

•

?amp%a#

2. P&'!itn(an Kada' 1. M&nt!o" )ada s! *o"o# 1+00 ,

01

 01 G '  01 G  m  00 m

00;

 00; G '  00; G  m  03; m 41,7

 N



)

 A + 1,25 B

41,7



(

tR 2 Wo , 1

1,3 0,5

( )

+

9,7 1,9

1,25

1084,617



3,85

 28118

 

 L   N 

:ETP

2



281,7187

 0001

T/



W   0,05 2 f  2,4 cm



2 x 1 cm

 12 m 2. ,a#)'an )ada s! *o"o# 1+00 , H (1)  H G '   H G !!   22 m

H (2)  H G '   H G ;9   29; m 2 ( tR 2 −tR 1 )

R



1,699 ( W  ½ ( 1 )+ W  ½ ( 2 ) ) 2 ( 10,1−7,7 )

R



1,699 ( 1+ 1,1) 4,8

=I.

R



R

 13!;3

3,5679

PEBA:ASAN Pa*a pak$k%m kal # *lak%ka# a#al,, koma$o&a/ &a, *e#&a# ,ampel me#$ol kam/e *a# amp%a##a *e#&a# ,%'% kolom 1!0 0 ? *a# 1;0 0 ?. Koma$o&a/ &a, a*ala' po,e, pem,a'a# amp%a# me#7a* kompo#e#4 kompo#e##a *e#&a# me#&&%#aka# &a, ,ea&a /a,e &eak a#& mele+a$ ,%a$% lap,a# ,eapa# a#& *am. Pa*a %m%m#a ke&%#aa# koma$o&a/ &a, a*ala'

%#$%k melak%ka# pem,a'a# *a# *e#$/ka, ,e#a+a a#& m%*a' me#&%ap ,e$a melak%ka# a#al,, k%al$a$/ *a# k%a#$$a$/ *alam ,e#a+a amp%a#. P#,p koma$o&a/ &a, pa*a *a,a#a ,ama *e#&a# p#,p :PL? kae#a ,ama4,ama me#&&%#aka# kolom 'a#a ,a7a pa*a koma$o&a/ &a, ,ampel a#& *#7ek, 'a%, $a'a# pa#a, kae#a me#&&%#aka# &a, pemaka.  pa*a koma$o&a/ &a, ,ela# ole' a/#$a,#a $e'a*ap /a,e *am ma%p%# /a,e &eak pem,a'a##a 7%&a *$e#$%ka# ole' $$k **' kea$,a# *a ,ampel. Pak$k%m kal # e$%7%a# %#$%k me#&e$a'% pe#&a%' $empea$% kolom $e'a*ap +ak$% e$e#, ($R). Pa*a pak$k%m koma$o&a/ &a, I keepa$a# ala# &a, a#& ak **apa$ pa*a keepa$a# ala# 1;0 mlme#$. Se'#&&a pa*a  pak$k%m kal # koma$o&a/ &a, II *&%#aka# keepa$a# ala# 1;0 mlme#$ *e#&a# pea#*#&a# ,%'% kolom 1!0 0 ? *a# 1;0 0 ?. Pa*a *a$a 'a,l peoaa# **apa$ka# 'a&a N me#$'ol pa*a ,%'% kolom 1!0 0 ? *a# 1;00 ? a*ala' 28118 *a# 3283;. Se*a#&ka# 'a&a N kam/e pa*a ,%'% kolom 1!0 0  ? *a# 1;0 0 ? a*ala' 2!881 *a# 222!. "ka 'a&a N le' e,a  maka 'a,l le' ak. :a&a N me#$'ol pa*a ,%'% kolom 1!0 0 ? ma%p%# 1;0 0 ? le' e,a *a kam/e. "a* me#$'ol le' ak *a kam/e. =II.

KESIPULAN Be*a,aka# 'a,l peoaa# *apa$ *,mp%lka# a'+a 1. :a&a N me#$'ol pa*a ,%'% kolom 1!0 0 ? ma%p%# 1;0 0 ? le' e,a *a kam/e. "a* me#$'ol le' ak *a kam/e. 2. :a&a N me#$'ol 1!0 0 ?  28118 :ETP 0001 T/  12 m. 3. Nla R amp%a# 1!00 ? a*ala' 13!;3

=III.

-AFTAR PUSTAKA '$$p+++.lo&pa*.om200911koma$o&a/4&a,.'$ml http://www.sodiycxacun.web.id/2010/01/kromatografi-gas.html#axzz1dkluplp http://www.scribd.com/doc/2"$%2/&'()*'+-(*',-(emisahan-dan(enentuan-omponen-)rganik-dengan-romatografi-as