Uji tentang adanya protein dalam suatu sampel dilihat daro konsentrasinyaFull description
Uji tentang adanya protein dalam suatu sampel dilihat daro konsentrasinyaDeskripsi lengkap
DOWNLOAD PDF-nya DI : http://adf.ly/be7S9 UJI BIURET
DOWNLOAD PDF-nya DI : http://adf.ly/be7S9 UJI BIURETFull description
Laporan praktikum biokimia protein biuret
DETERMINACION DE PROTEINAS POR EL METODO DE BIURETDescripción completa
There are two points as the aims of this experiment: (1) To identify protein by using peptide bond at protein through biuret test, sulfur test, precipitate by metal, precipitate by salt and protein...Full description
cloze trstFull description
Descripción: asd
Practice Test for gre
Biuret Test for Proteins The Biuret Test is often used to determine the presence of peptide bonds in protein. At this level you will be testing for the presence of protein in foods. The test may also be extended to quantitatively measure the concentration of total protein. protein . ➔ Instead of the Biuret Reagent, Reagent , the following may be used •Fehling’s •Sodium
Solutions A and A and B
hydroxide and copper (II) sulphate solutions
Procedure 1.
Add ! cm" of the liuid food sample# sample # to a clean, dry test tube
!. Add Add ! cm" of of Biuret Reagent. Reagent . Alternatively ". $se sodium hydroxide solution and solution and copper sulphate solutioninstead. solution instead. Add % cm" of sodium hydroxide solution &'() or bench solution* and %) copper &II* sulphate solution dropwise + drop by drop to the food sample '. $se Fehling!s A and B solutions instead. solutions instead. -ehlings A and B should be freshly prepared. -ehling/s A is copper &II* solution and -ehlings B is a mixture of sodium potassium tartrate and sodium hydroxide solution. %cm" of each solution + A and B + should be added to the food sample. 0. 1epea 1epeatt step steps s % and and ! with with de"ioni#ed $ater to $ater to prepare a negati%e controland control and with albumin &egg albumin &egg white* to prepare a posti%e control. control . 2. Sha&e $ell and $ell and allow the mixture to stand for ' minutes. minutes . 3. bser%e any bser%e any color change. * Prepare liquid samples from solid foods. Crush the solid food, add a little de-ionized water and decant the liquid. This liquid should be used for the food test. The quantity of food crushed and water used depends on o n the number of tests to be conducted.
bser%ations and Interpretation
TEST
P45ITI67
97:ATI67
OBSERVATION
The solution turns from blue to violet& purple* The solution turns from blue to pin8 9o change ;The solution remains blue
INFERENCE
Proteins are present Peptides are present & Peptides or peptones are short chains of amino acid residues* 9o protein is present
