M514 M516 AU400
Set Up Manual Rev. 0500
Tec Tech hnical ical Supp Support ort Gr Grou oup p Global Business Technical Support Department Beckman Coulter K.K
Revision Issued 0100 0200 0300 0400 0500
Cover page page
Date 1998.06.05 2000.04.30 2000.09.05 2002.01.31 2009.09.10 2011.01.07
Revision
Date
ST_ ST _ CV_ 1.DOC
A U400 U400
Se t Up M A NUAL NUA L HIST HISTO RY TA BLE P.
REV. REV.
PAG PA G E
REPLAC REPLAC E
0100 0100 0200
Cover Cover 0-1 1-5 1-6 4-9 7-1 7-2 7-3 7-4 7-5 7-6 7-7 1-1 Cover TC 1-1 4-2 4-4 4-7 4-9 Chapter hapter 10 Cover
Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Yes Y es Y es Y es Y es Y es Y es Y es
0300 0400
0500
HISTO RY Addition Addition (Rev. (Rev. 0100) 0100) Addition Addition (Rev. (Rev. 0200) 0200) Addition Addition (4-9, 7-7) Addition Addition (ME6332 (ME 6332 Tray) Addition (Name plate) plate) Addition Revise Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Addition of attention attention matter matter Addition Addition (Rev. (Rev. 0400) 0400) Add Ad ditio ition n (Chap Chapter 10) Addition Addition (Spec. (Spec. of AU403,AU404(M516 AU403,AU404(M516)) )) Addition (Warning (Warning Caution) aution) Modified figures. figures. Changed the destination name name Addition (Instruction) (Instruction) Addition (Add memo emo (Except (Except AU403 AU403,AU ,AU404 404)) )) Newly created created Company ompany name name changed
1
A U400 U400
Se t Up M A NUAL NUA L HIST HISTO RY TA BLE P.
REV. REV.
PAG PA G E
REPLAC REPLAC E
0100 0100 0200
Cover Cover 0-1 1-5 1-6 4-9 7-1 7-2 7-3 7-4 7-5 7-6 7-7 1-1 Cover TC 1-1 4-2 4-4 4-7 4-9 Chapter hapter 10 Cover
Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Y es Yes Y es Y es Y es Y es Y es Y es Y es
0300 0400
0500
HISTO RY Addition Addition (Rev. (Rev. 0100) 0100) Addition Addition (Rev. (Rev. 0200) 0200) Addition Addition (4-9, 7-7) Addition Addition (ME6332 (ME 6332 Tray) Addition (Name plate) plate) Addition Revise Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Revise (Page (P age No) Addition of attention attention matter matter Addition Addition (Rev. (Rev. 0400) 0400) Add Ad ditio ition n (Chap Chapter 10) Addition Addition (Spec. (Spec. of AU403,AU404(M516 AU403,AU404(M516)) )) Addition (Warning (Warning Caution) aution) Modified figures. figures. Changed the destination name name Addition (Instruction) (Instruction) Addition (Add memo emo (Except (Except AU403 AU403,AU ,AU404 404)) )) Newly created created Company ompany name name changed
1
M514
SET UP MANUAL TABLE OF CONTENTS
0-1 0400
0200
1.
Set up Preparation ..........................................................................
1-1 to 1-6
2.
Set up Flow .....................................................................................
2-1
3.
Unpacking ....................................................................................... .......................................................................................
3-1 to 3-4
4.
Set up Procedure ............................................................................
4-1 to 4-9
5.
Set up personal computer ..............................................................
5-1
6.
Processing the heater line of JD....................................................
6-1
7.
Operation Check ............................................................................. .............................................................................
7-1 to 7-7
8.
Test running ...................................................................................
8-1 to 8-2
10. ISE Set up .......................................................................................
10-1 to 10-7
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M514
SET UP MANUAL
Set up preparation
I
1-1 0400
0300
Installation Environment Conditions G Installation Environment Conditions
• • • • • •
Site not subject to direct sunlight Site not minimum amount of dust Site that is flat (gradient : less than 1/200) Site with minimum vibration Site with floor construction that can support approximately 480Kg (4902N) Places located less than 2000m above sea level
G Power and noise Conditions
• • • • • G
Site which be located so that 1 0m water supply ad drain hoses Site with a power source with minimum voltage fluctuation (+-10%) Site the switchboard which is located so that a power cable with a length of 10m will reach the system. A place with no institution sending the signal signal of vertical polarization of 280 to 300 MHz to the neighborhood (The police, a fire department, and public institution may be equivalent) A place with no corrosive gas.
Temperature Temperature and humidity conditions • •
The temperature of the installation room should be between 18 and 32 °C, and the temperature fluctuation during analysis should be +-2° +-2 °C. (the calorific value of the environments environments : approx. 2600Kcal/H) 2600Kcal/H) The humidity must be between 40 and 80%RH with with no condensation. condensation. (Humidity condition condition for installation : between 5 and 40 °C)
G Water Supply and Drainage conditions
•
• • • • •
• •
An aqueduct gate must be within 10 m of the water supply gate of the instrument and the water waste gate must be under 0.1 m in height. (If the optional forced waste water unit is used, a height of 1.5 m is required.) Inner diameter of water waste gate must be greater than 48 mm. The purity of DI water must be under a conductivity of 2.0 us/cm. (The CAP type 2 level.) The temperature must be greater than 5 °C. The pressure in the aqueduct must be between 49 and 392 KPa. (0.5 to 4 kgf/cm) (Caution ! : If the water pressure is greater than 2 kgf, attach a water supply unit.) unit.) Average amount of water required Unit types AU401, AU402 (M514) : 26.0 L/h (50/ 60Hz) Unit types AU403, AU404 (M516) : 20.0 L/h (50/ 60Hz) Maximum instantaneous instantaneous amount of water required : 0.7 L/min. (50/ 60Hz) Kind of waste liquid Concentrated waste liquid (sample + reagent) : 2.0 L/h (including reagent) Washing waste liquid : 24.0 L/h (Unit types: AU401, AU402 (M514)) Washing waste liquid : 18.0 L/h (Unit types: AU403, AU404 (M516))
ST01_01
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SET UP MANUAL
1-2
Set up preparation
I
Space requirements G As shown in the figure below, this system requires space around it for safe installation and maintenance.
G
G
Dimensions of system main units (Unit:mm) Analyzer
Width 1450 x Depth 760 x Height 1210
DPR
Width 700 x Depth 700 x Height 1430
Power supply Voltage USA
110V
Europe
230V
Australia
240V
Asia
110V or 220V
Japan
AC100 or 200V
Mono phase
50/60 Hz+-1Hz
Voltage change
Within +-10%
Electric power 3.5KVA
Switchboard The switchboard should be located so that the standard po wer supply cable(10 m) will reach the instrument.
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SET UP MANUAL
1-3
Set up preparation
Options There are specific optional device models depending on where the instrument will be used.
No.
Name
MU4970 MU4971 MU4972 MU4986 MU4702 MU4148 MU3351 MU2535 MU0815 MU4146 MU4147 MU1853
M514-OPA01 M514-OPA02 M514-OPA03 M514-OPA04 M520-OPH02 M520-OPH01 M240-OPH03 M240-OPD01 M250-OPE01 M520-OPE01 M520-OPE02 M240-OPH01
Jap an (1)
USA
Euro pe
Asia
Aust ralia
(1) (1) (1) (2) 2 (2) (1) (1)
(1)
(1)
(1)
(1)
(1) (1)
Contents ISE unit ISE unit ISE unit ISE unit Forced waste water unit (for CSA) Forced waste water unit (for LVD) Forced waste water unit Water supply unit (for CSA) Water supply unit Water supply unit (for CSA) Water supply unit (for IEC) Forced waste water unit
ST01_01
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SET UP MANUAL
1-4
Set up preparation
Checking the contents of the package Name ANL main body Cable set PC rack Floor plate Standard accessories Starting kit Package type
Japan 1 1
USA 1
Europe 1
Asia 1
Australia 1
4 1
4 1
4 1
4 1
1 1
1 1
1 1
1 1
1
4 1 1
inner outer
1
(Special accessories) *
For information about the main configuration, refer to the Function and Mechanism manual.
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SET UP MANUAL
1-5
Set up preparation
0200
Standard accessories list No. MU8092
Name NE rack W
Japan 1
USA 1
MU8093 MU8094 MU8095 MU8096 MU8097
NE rack B NE rack G NE rack R NE rack Y NE rack O
1 1 1 1 1
MV2313 MV2314 MV2315 MV2316
PROGRAM PROGRAM PROGRAM PROGRAM
1
MV1764
1
20 20 20 2 2 2 4 1
MU9066 MU9067 MU8051
Communicatio n cable Template Code set Cuvette S probe R probe Luminous lamp Partition Partition Partition Coolant B hose B hose Band A letter of guaranty Binder Operator’s manual Operator’s manual Rack ID label Rack ID label Rack ID label
1 1 1
1 1 1
MU9413 ZM0227 ME6332
Stylus wire Stylus wire Tray
1 1 1
1 1 1
MU8108 DW4114 ZM0634 MU9934 MU9958 MU9888 ME3333 ME4610 ME4611 ME4305 MB9963 MB9961 MA7528 MA1198 ME4498 ME5234 ME6164
1 1 1 1 1
Europe 1 1 1 1 1 1
Asia 1
Australia 1
1 1 1 1 1
1 1 1 1 1
1
1
1
1
φ 11.5 to φ16.0 10
pcs/set φ 11.5 to φ16.0 φ 11.5 to φ16.0 φ 11.5 to φ16.0 φ 11.5 to φ16.0 φ 11.5 to φ16.0
1 1
1 1 1 1 1 1
1
1
125V, 3A, 3m 10 pcs/ set
1 1 1 1
1 1 1 1
1 1 1 1
1 1 1 1
20 20 20 2 2 2 4
20 20 20 2 2 2 4
20 20 20 2 2 2 4
20 20 20 2 2 2 4
1
1
1
1
1 1 1
1 1 1
1 1 1
No. 1 to 20 No. 21 to 40 No.1 : 14 pcs No. 2, 3: 3 pcs
1 1
1 1 1
1 1 1
φ 0.2 φ 0.3
1 1
For 30 ml bottle inside For 15 ml bottle inside For 15 ml bottle outside φ 12 x 18 x 10 m φ 15 x 22 x 10 m
For GA drain
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SET UP MANUAL
1-6
Set up preparation
0200
Standard accessories list
No. MB0754 GB9219
Name Wrench 2.5 Wrench 1.5
Japan 1 1
MU8117 MU8118 MU8119
Adapter C Adapter A Adapter B
1
MU8120
Seal
1
MD7304 MD7305 ME4609 MD0884
Bottle 60 Bottle 30 Bottle 15 Cap A
1
ZM0062
Sample cup
ME9382 ME9383 ME9384 ME9385 ME9430
Name plate Name plate Name plate Name plate Name plate
USA 1 1
Europe 1 1
Asia 1 1
Australia 1 1
1
1 1 1
1 1 1
22 pcs/ set ME2272 22 pcs/ set ME2175 22 pcs/ set ME2176
1
1
1
1
Seal 4 types for DC cup identification
1
1
5 4 4 12
5 4 4 12
1
1
1
1
1
3 3 1 1 1
100 pcs/ set German French German French For ODC
ST01_01
M514
SET UP MANUAL
Setup flow
n
2-1
Setup flow
• • •
Selecting the instllation site Removing the packing material Placing the equipment at the installation site
• •
Leveling the equipment Jacking up the equipment
• • •
Wiring from AU400 power Wiring for PC, printer, etc Plumbing for supply and waste water
•
Pouring coolant (forincubation bath) Coolant for the DB (cooling unit) has been poured at the factory before shipment Setting the master detergent
• ** • • •
Power ON(PC only) Check the BIOS Program install Setting of System Maintenance (Refer to System Maintenance manual) *** After setting, turn off the power •
Process the heater line of the JD portion (to prevent empty burnig)
** Power ON • Position check by DIAG • After position check attach the consumables • Air extraction *** Power OFF •
Return to processing for heater line of JD
** Power ON • W1/Photocal • Water test *** Analysis test •
Set up complete
st02_01.doc
M514
SET UP MANUAL
3-1
Unpacking
n
Unpacking lRemoving
the outside packaging materials
1) Loosen the band. 2) Remove the L angle.
3) Remove the ceiling lid. 4) Use a pair of pliers to remove the joint securing the body frame. 5) Loosen the wire securing the body of the AU400 to the wood frame. 6) Lift the AU400 off the wood frame.
ST03_01.DOC
M514
SET UP MANUAL
3-2
Unpacking
n
Opening the package lRemoving the inside packaging materials 1) Loosen the bands, and remove the vinyl sheet. 2) Remove the grip bar.
3) Remove the all pads to protect the cover. 4) Remove the packaging material for the front door.
5) Remove the screw for fixing the back door. 6) Remove the mouthpiece cover.
ST03_01.DOC
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SET UP MANUAL
3-3
Unpacking
7) Remove the packaging fixed to each unit. 8) Peel tapes fixed to the CB(mirror unit). 9) Remove the vinyl sheet fixed to Float SW. 10) Peel the other tapes.
ST03_01.DOC
M514
SET UP MANUAL
Unpacking
n
3-4
Standard accessories
ST03_01.DOC
M514
SET UP MANUAL
Set up procedure
I
4-1
Leveling 1) Position the floor plates under the jacks. (Position the plates squarely.) 2) Jack up the 4 casters at the same time. If a caster isn’t in contact with the ground, use a level to check that it is level. * Open the front door and place the level on the frame. When checking with a level, place this instrument on the frame after first opening the front door. 3) Use the jacks to make the instrument level.
ST04_01
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SET UP MANUAL
4-2
Set up procedure
I
0400
Wiring the power supply G Wiring the power supply cable between the AU400 and the switchboard of the facility.
! Warning 1. To prevent a short circuit when repairing or assembling, turn off the main power, and wait at least 10 s econds before starting work. 2. To prevent electric shocks and short circuits do not touch electric parts and wiring with wet hands. 3. Connect grounding terminals otherwise electric shocks or errors may occur. 1) Remove the door on the rear side of the instrument. 2) Connect the power supply cable in the region labeled A in Fig. A. (The power supply unit is housed here.)
Region A 3) Connect the power supply cable to the switchboard of the facility. * Check that the voltage of the facility is Live (Hot) or Neutral (Cold) using a tester surely before the power supply cable is connected. Connect this terminal to * Grounding resistance : Less than 100ohm Supply power that grounding terminal conforms to the (Grounding terminal: less than instrument specifications 100 ohm
! Caution Grounding terminal should be connected to the grounding terminal its grounding resistance is less than 100ohm.
ST04_01
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SET UP MANUAL
4-3
Set up procedure
I
Wiring for personal computer Make the following connections to the personal computer. G
CRT & PC CRT
Connect to
Power supply
[Constant power supply output] for PC
AUDIO IN
[LINE OUT] connector for PC
CRT signal
[Display] connector for PC
G
PC and standard accessories PC
Power supply
Connect to Output from JD portion of ANL If the PC and printer are both connected to the J D portion, split the power using the table tap.
Keyboard
[KEYBOARD] connector for PC
Mouse
[MOUSE] connector for PC
G
PC and standard printer Printer
Power supply
Connect to Output from JD portion of ANL If the PC and printer are b oth connected to the JD portion, divide the power by the table tap.
Printer signal G
[Parallel] connector for PC
PC and ANL
PC (Communication with ANL) Serial connector 1 G
Connect to [COM] connector at JD portion of ANL
Special accessories and others Option
Connect to
Option printer
[Parallel] connector at extension board of PC
Touch panel signal
[Serial] connector at extension board of PC
Online
[Serial connector 2] of PC
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SET UP MANUAL
4-4
Set up procedure
I
0400
Installation of the personal computer G CRT & PC
CAUTION 1. Do not install the personal computer in an unstable location. There is the danger of injury from dropping. 2. Do not close the air hole. There is the danger of fire from a stuffed air. 3. Do not istall the personal computer at a location where it is exposed to water or other liquids. There is the danger of erroneous operation or fire.
G Connection of the personal computer
CAUTION 1. To prevent electric shocks and short circuits do not touch electric parts and wiring with wet hands. 2. Do not insert the power plug into a receptacle while dust or other foreign matter adheres to the plug. There is the danger of fire. 3. Insert the power plug positively all the way. 4. Contact all cables correctly. A wrong connection can cause trouble.
Connect the ports of the personal computer according to the following table. For other connections than the following ones, follow the manual for the personal computer. PC
Connecting
Remarks
Serial 1
ANL
Serial 2
(Host computer)
Serial 3
Modem
Option
Serial 4
Touch panel (expect for America)
Option
Parallel 1
Printer
Parallel 2
Optional printer
Option
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SET UP MANUAL
Set up procedure
I
4-5
Plumbing for supply and waste water General diagram G Connecting to supply water hose, concentrated waste liquid waterh hose, washing waste liquid water
hose, and exhaust air hose. 1) Connect to the blade hoses to the mouthpiece on the rear face. (See the below figure.) Concentrated liquid water, Washing waste liquid water : Blade hose 15 x 22 Supply water, exhaust air : Blade hose 12 x 18
Instruction 1. Connect to all the hose of concentrated / washing liquid waste, water supply, and exhaust air because a little water is drained from the exhaust air hose. 2. Do not fold or pinch a drain hose and an exhaust hose, and keep the hose ends open to air. 3. Do not close up a drain hole in which the hoses are inserted.
ST04_01
M514
SET UP MANUAL
Set up procedure
I
4-6
Connecting the deionizer (1) Connection for MU2535 (M250-OPE01) type * Allow the deionizer to be connected by an expert distributors.
(2) Connection for MU0815(M240-OPD01) type : for Japan Connection for MU4146(M520-OPE01) type : for USA Connection for MU4147(M520-OPE02) type : for Europe * Allow the deionizer to be connected by an expert distributors.
ST04_01
M514
SET UP MANUAL
4-7
Set up procedure
I
0400
Connecting the forced waste water unit and water supply valve G Connecting the forced waste water unit
1. Connecting the hose of the forced waste water unit.
Connect the waste water line from the main body (hose) to the “ IN ” connector of the forced waste water, and connect the hose from the “ OUT ” connector to the waste gate of the facility. (The drain hole : 1.5m or less) (See Fig. A below.)
Connect the tube from the concentrated waste liquid to o ne forced waste water unit(1st).
Put the accessory stopcock to the mouthpiece in one forced waste water unit(1st).
Connect the hose from the washing waste liquid and the exhaust air to another forced waste water unit(2nd).
2. Connecting the power supply of the forced waste water unit
Connect the power source line and the signal line to the connector of each JD portion. (See Fig B below)
Instruction Keep the hose ends open to air.
G Connecting the water supply valve (option)
1. G type (solenoid valve type) Connect the tube from the source of the aqueduct to the “ IN ” connector of the solenoid valve, and connect the tube from the “ OUT ” connector to the input line of the deionizer.
Connect the control line of the solenoid valve to the connector of the JD portion. (See the Fig.B below)
* If the resin bomb (cylinder) type deionizer is used, do not connect the solenoid valve to the exit side of the resin bomb, or the bomb may accidentally burst. Connect it to the entrance side. 2. Mini clear type
Connect the control line of the solenoid valve to the connector of the JD portion. (See the Fig.B below)
Fig. A Fig. B
ST04_01
M514
SET UP MANUAL
Set up procedure
I
4-8
Pouring the coolant 1) Open the cover A. (see the figure below) 2) Remove the stopcock under the cover A, and pour the coolant from the gate. 3) Set the stopcock to the gate. 4) Turn on the environments, and check the coolant level. * Refer to following pages how to set up the environments. 5) Set the cover A (see the figure below).
I
Setting the original detergent 1) Set detergent A and detergent B as shown in the figure below.
ST04_01
M514
SET UP MANUAL
Set up procedure
I
4-9 0400
0200
Installation of Drain tube of incubation bath 1) Drain tube of incubation bath is put in the tray. (ME6332) 2) The tray with the tube is put under the AU400. * Except Europe (Unit types AU401, AU402(M514)) and AU403, AU404(M516)
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SET UP MANUAL
Set up personal computer
n
5-1
Setting up the personal computer 1. Turn on only the PC. Do not turn on anything else. 2. Setting the BIOS * For detailed setting information refer to the system maintenance manual. 3. Installing the AU400 program * For detailed setting information refer to the system maintenance manual. 4. System maintenance setting * For detailed setting information refer to the system maintenance manual. 5. If the setup of the PC has been completed, turn of it.
st05_01.doc
M514
SET UP MANUAL
Processing the heater line of JD
n
6-1
The purpose for processing the heater line of the JD portion l
A new instrument has not primed for the water in the plumbing line. Therefore if the power is turned on for the first time, the temperature fuse of the heater for wash water heating may trip. Follow the procedure below to prepare the heater line of the JD portion and prevent the fuse from tripping.
*Note that an error is not issued if the fuse is tripped. n
Preparation procedure 1)
Detach the heater wire from the terminal table of the JD portion on the rear left side of the ANL. (See the below figure A.)
2)
Insulate the detached wire with vinyl tape.
st06_01.doc
M514
SET UP MANUAL
Operation check
n
0200
7-1
Turning on the power l
If the PC has been set up and the JD portion has been processed, turn on the instrument and check the position and action of each unit.
1. Air extraction of DI water supply pump 1) 2) 3) 4) 5) 6) 7) 8) 9) 10) 11) 12) 13) 14) 15) 16) 17)
The front cover opened, and F cover is removed. The connector of the DI water supply pump is removed. The detergent is put in the Detergent tank. (Detergent diluted to 2%.) Turn on the AU400 power. Please wait until the DI tank becomes being filled with water. The filter is removed from the fixing board. The filter is shaken, and water is poured in the pump. The connector of the DI water supply pump is connected. The FC sequence of DIAG menu is done. C insert is removed from C body when doing for a while. Water is turned on pushing the point of C insert to a big container. C insert is installed in C body. The filter is shaken, and sir on the inside is pulled out. Afterwards, water is made to often circulate. The FB sequence of DIAG menu is done. At this time, if the noise dose not do from the DI water supply pump, it is completion. F cover is installed.
CAUTION The bearing wears out if this work is not done.
ST07_01.DOC
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SET UP MANUAL
Operation check
0200
7-2
2. Air extraction of coolant line 1)
The tube of the circulation pump for GA unit in the back of AU400, and air in the tube is pulled out.
CAUTION 1. 2.
The error ( INCUBATION BATH TEMP . ERROR etc.) might occur when there is air in the tube. Please confirm there is no air in the tube.
ST07_01.DOC
M514
SET UP MANUAL
Operation check
n
n
0200
7-3
Loading the consumables l
Load the sample probe, reagent probe, and mixing bar, by checking the position with the DIAG menu. Do not load these consumable parts before performing the position check, because the position shift may have been caused problem.
l
The DIAG menu does not have to be used if attaching the adapter for DC unit(the cooling sampler) and recording paper.
Position check using DIAG menu
1. Check the horizontal position of the sample probe. 1) Check the sample probe alignment in the cuvette. (Fig. A). 2) Check the sample probe alignment in the wash pot. (Fig. B). 3) Check the sample probe alignment in the sample cup(Rack, DC unit). (Fig. C). 4) Check the sample probe alignment in the ISE sample pot. (Fig. D). * Check each position and if necessary, adjust according to the Adjustment manual.
A : Cuvette position
C : Sample cup position
B : Wash pot position
D : ISE position
ST07_01.DOC
M514
SET UP MANUAL
Operation check
0200
7-4
2. Check the horizontal position of the reagent probe. 1) Check the reagent probe alignment in the cuvette. (bellow fig. A). 2) Check the reagent probe alignment in the wash pot. (bellow fig. B). 3) Check the reagent probe alignment in the reagent cup. (bellow fig. C). 4) Check the reagent probe alignment in the dilution bottle. (bellow fig. D). * Check each position and if necessary, adjust according to the Adjustment manual.
B : Wash pot position A : Cuvette position
C : Reagent bottle position
C : Dilution bottle position
ST07_01.DOC
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SET UP MANUAL
Operation check
0200
7-5
3. Check the horizontal position of the mixing bar. 1) Check the mixing bar alignment in the cuvette. (bellow fig. A). 2) Check the mixing bar alignment in the wash pot. (bellow fig. B). * Check each position and if necessary, adjust according to the Adjustment manual.
A : Cuvette position
B : Wash pot position
ST07_01.DOC
M514
SET UP MANUAL
Operation check
0200
7-6
4. Check the dead volume for the sample probe and reagent probe. l
Dead volume at rack position Type Hitachi cup
l
50µL or less
ACA cup
250µL or less
φ13 blood collection tube
200µL or less
φ15 blood collection tube
250µL or less
Dead volume at the DC unit position Type
l
Dead volume
Dead volume
Cup for small quantity
30µL or less
Hitachi cup
50µL or less
Hitachi cup
50µL or less
Conical cup for STAT
50µL or less
ACA cup
250µL or less
φ13 blood collection tube
200µL or less
φ15 blood collection tube
250µL or less
Dead volume at the reagent bottle Type
Dead volume
15 mL bottle
350 µL or less
30 mL bottle
1mL or less
60 mL bottle
1mL or less
* Check each position and if necessary, adjust according to the Adjustment manual.
ST07_01.DOC
M514
SET UP MANUAL
Operation check
n
0200
7-7
Air extraction • After making all the position checks, as described on the previous page, recheck the probe and mixing bar attachment. Carefully check for looseness in the connections of the sample and reagent probe connections • Check the syringe connection (loose the syringe case or tubes). 1) The sample and reagent dispenser is moved up/down and the air is extracted using the ANL maintenance menu. * If the air isn’t extracted, detach the syringe case in the dispenser and extract the air by swaying the syringe by hand.
Air extraction position
Menu
Plumbing for sample line
Maintenance Ü ANL maintenance Ü S/ R dispenser syringe replacement
Plumbing for reagent line
Maintenance Ü ANL maintenance Ü S/ R dispenser syringe replacement
2) The mix sequence is executed and the outer wash water of the mix wash pot is filled using the ANL maintenance menu. Air extraction position Plumbing for mix outer wash
Menu Maintenance Ü ANL maintenance Ü Mixing bar replacement
3) The wash nozzle sequence is executed and the wash water is filled using ANL maintenance. Air extraction position Plumbing for wash nozzle line
Menu Maker maintenance Ü ANL maintenance Ü Replacing Wash Nozzle
Caution The cuvette may be splashed by water from the nozzle the first time because of air in the tube. In the first time, detach the nozzle portion necessarily and accept by the container. Once the air has been extracted, replace the nozzle and check the wash condition by discharging the real wash water into the cuvette.
4) The probe wash sequence is executed and the wash water is filled using ANL DIAG. Air extraction position Plumbing for clot detection sensor
Menu Maker maintenance Ü ANL DIAG Ü Probe wash
If the water test (analysis test) is executed without the air having been completely removed the data may not be reliable. Be sure to extract all the air.
n
Processing the heater line in the JD portion 1) If the air has been extracted, turn off the power. 2) Execute the processing for the heater line of the JD portion described on page 6-1.
ST07_01.DOC
M514
SET UP MANUAL
8-1
Test running
n
n
Executing W1 1)
If processing of the heater line of the JD portion has been completed, turn on the instrument again.
2)
When the instrument starts, execute W1.
*
For the W1 action, check the wash water discharge and aspiration. (It is easy to check if the cover of the GA portion is detached.)
Executing photocal 1)
If W1 has been completed, execute photocal by closing the GA portion cover and the main body cover.
2)
If photocal has been completed, check the cuvette error.
If the average value of the check range is 0.03, there is no cuvette error.
3)
If a cuvette error has occurred, check the applicable cuvette and execute photocal again after cleaning or replacing it.
4)
Keep executing until there is no cuvette error.
st08_01.doc
M514
SET UP MANUAL
8-2
Test running
n
Water test l
If W1 and photocal have been completed, execute the water test as follows and check the photometry line performance.
1)
Enter the parameters as follows.
2)
Prepare degassed water (if it is difficult to obtain, use DI water instead) with P8P (triton 100X) to 0.1%. (88 samples x 4 tests).
NAME
340nm
380nm
480nm
600nm
CH
R1=2
R1=4
R1=6
R1=8
Amount for sample dispensing
2
2
2
2
Amount for sample dilution
0
0
0
0
Force- multiplier of sample prior-diluent
1
1
1
1
Amount for R1 dispensing
50
50
50
50
Amount for R1 dilution
100
100
100
100
Amount for R2 dispensing
0
0
0
0
Amount for R2 dilution
0
0
0
0
Measurement wavelength (main)
340nm
380nm
480nm
600nm
Measurement wavelength (sub)
410nm
520nm
660nm
800nm
Analysis method
END 1
END 1
END 1
END 1
Reaction slope
+
+
+
+
Measurement point 1 1st
1
1
1
1
Measurement point 1 Lst
27
27
27
27
CAL Type
MB
MB
MB
MB
10000
10000
10000
10000
Factor * Others : Set the maximum value, etc.
* Name, CH, and the reagent bottle position : Set in position optionally. 3) Set the 88 sample racks in white cups and put DI water in each cup. 4) Put the degassed water with triton (made in item 2) into the reagent bottle, and place one in the refrigerator. 5) The 4th requisition of the 88 samples is registered in normal test requisition in the routine menu. 6) Start analysis. 7) The data must satisfy the standard below.
Standard
The range of 340/410nm, 380/520nm : less than 15 for all channels. The range of 480/660nm, 600/800nm : less than 10 for all channels.
n
Test running
Be sure to perform a water test to check the conforming standard if the photometry and the dispenser system are running correctly.
st08_01.doc
M514
SET UP MANUAL
10-1
ISE Set Up
I ISE G
0400
Set up
Set up procedure 1)
Power off AU400.
2)
Remove covers A , B , C , D , E , F and G. (See Fig-1)
Pillars
Fig- 1
3)
Remove the front cover and the side cover.
4)
Remove pillars under cover A.
5)
Attach MV2190 to the PCB cover and then attach the PCB ass’y under cover F.
6)
Plug in all the connector under cover F to MV2190.
7)
Mount the ISE unit at the location of cover A. Before mounting the ISE unit, please remove the tube (Flesh color tube)
8)
Un-bundle connectors bundled under cover A.
ST10_01
M514
SET UP MANUAL
10-2
ISE Set Up
9)
0400
Plug in connectors of ISE unit. [132][129][136][130][126] (See Fig-2)
10) Plug in connectors to MV1040. Fix a terminal from connector [122] with screw for fixing PCB. [111][122][112][133][134][Cable from electrodes]
Fig- 2 1. Connector 113 on MV1041 is vacant. (This is for PCB checking at our factory) 2. Power cable No.”130” is not using. 11) Mount reagent tray and mouthpiece plate ass’y. Plug in connectors belong to reagent tray. [123][124][125] (See Fig-3)
Fig- 3
ST10_01
M514
SET UP MANUAL
10-3
ISE Set Up
0400
12) Mount SA unit ( Dispenser ) to location A in Fig-4 and plug in connectors. Mount valve at location B and plug in connector [131]. Mount ISE switches ass’y at location C and plug in the connector.
Cut the grommet. Pass the tube and cable through this grommet.
Fig- 4 13) Insert drain tubes from ISE unit to Y shape connector and fix them with tie lap (SKB band).
Put tubes marked with mark band “blue” and “yellow” to mouthpiece plate ass’y fixed in the previous step. Put tube marked with mark band “8” to [NC] of valve fixed in the previous step through upper part of ISE reagent tray.
Fig- 6
Fig- 5
ST10_01
M514
SET UP MANUAL
10-4
ISE Set Up
0400
14) Put tubes marked with mark band “red” and “7” . Put the tube with “red” mark to mouthpiece tray ass’y and the other tube with “7” mark to [ NO]. 15) Put tube remaining from [COM] of valve to SA unit. Close the other hole of syringe head with a cap.(See Fig-7)
Fig- 7
16) Fix all covers removed in the previous steps.
ST10_01
M514
SET UP MANUAL
10-5 0400
I Position check
Refer to Adjustment manual.
ST10_01
M514
SET UP MANUAL
10-6
ISE Set Up
I
0400
ISE Calibration G
Check calibration Set ISE reagents and execute prime. Calibrate with the condition of the air is completely out.
G
Standard Calibration results of three times shall be in the following value.
Slope value
Factor value
Na
38 ~ 68
0.80 ~ 1.20
K
38 ~ 68
0.75 ~ 1.25
Cl
-38 ~ -68
0.75 ~ 1.25
CAUTION Take out the air from the line of samples and reagents.
ST10_01
