TRX Force Tactical Kit

TRX ForceTacticalKit www. pr os us pens i ont r ai ner .

Thi o r e do x i n 

pr o t e i ne s s e nt i alf o rl i f ei nmammal s

ac t sasanant i o x i dantt opr e v e nto x i dat i v e s t r e s s a c t sasane l e c t r o ndo no ri nav ar i e t yo f c e l l ul arr e ac t i o ns r e s t o r e so xi dat i v el yi nac t i v at e de nz yme s t ot he i rr e duc e d& ac t i v es t at e  www . pr o s us pe ns i o nt r ai ne r . c o m 

Thi o r e do xi ne xi s t si ne i t he rt her e duc e d o ro xi di z e df o r m H

H

S

S

S

S

Trx

Trx

Reduced Thioredoxin

Oxidized Thioredoxin

  LEGEND SH = thiol S-S = disulfide

Thi o r e do xi npar t i c i pat e si ndi t hi o l di s ul fidee x c hang er e ac t i o ns S

S

RNR oxidized

H S

H S

reduced Trx

H S

H S

RNR reduced

S

S

oxidized Trx

Thi o r e do x i nr e duc t as e& t hi o r e do x i n www. pr o s us pe ns i o nt r ai ne r . c o m thioredoxin H S NAD!

thioredoxin reductase NADH

H S

Trx

S

S

Trx

ribonucleotide reductase S S RNR

H S

H S

RNR

Hy po t he s i s 

Thi o r e do x i nr e duc t as ei sama j o rde t e r mi nanto f t hi o r e do xi nr e do xs t at e



I ft r ue ,t he nde l e t i o no ft hi o r e do xi nr e duc t as e s ho ul ds hi f tt her e do xs t at eoft hi o r e do xi nf r o m pr i mar i l yr e duc e dt opr i mar i l yo xi di z e d



www. pr o s us pe ns i o nt r ai ne r . c o m

Pr o t e i ne l e c t r o pho r e t i cmo bi l i t ys hi f tas s a y( PEMSA) SASASASH SASASH SASASASH SASASASH SASASA- acetone SH SASA"A# DTT SA- 'ash SH "AA SA- "odoaceta$ide TRX r e d

TRX r e d

TRX r e d

"odoacetic acid

TRX o x

SH SH SH SH S S

TRX r e d

Dithiothreitol

TRX o x

SASASASAS S

TRX o x

SASASASASH SH

LEGEND  A- = ne%ati&e char%e = Thioredoxin

# = neutral state S = Disulfide

TRX o x

SASASASAS# S#

Pr o t e i ne l e c t r o pho r e t i cmo bi l i t ys hi f tas s a y SH SH SH SH SH SH

Tr x r e d

SH SH SH SH S S

Tr x o x

DTT

   E    G    A       a   e   r   u

SH SH SH SH SH SH

"AA("A# "AA

-

)  -* -+ -, -

TrxSS

-. !

"A#

-/

TrxSH *

SASASASASASA-

S# S# S# S# S# S#

S# or SAS# or SAS# or SAS# or SAS# or SAS# or SA-

+

,



Re pr e s e nt at i v er e s ul t s 3ild T04e    0    l   o  n   A    "  A

   0    l   o  n    #    "  A

  *

+

,

#utant -

.

   1 ,

   )    ) +

  *    +   )

   2    +   +

   ,   )    +

  e  r   d   d    L  a

Trxox

$ixed disulfide

Trxred

Tr xoxidized

6

Mi x e d

12 10 14 13 15 17 17 20 21 18

d i s u l fi d e Tr xreduced

7

8

7

9

12 16 21 23 21

27 22 27 23 29 29 30 34 36 35

WT

Mut

Trxox

16%

26%

$ixed disulfide

28%

27%

Trxred

56%

47%

Co nc l us i o n 

Thi o r e do x i nr e duc t as ei sno tt hema j o r de t e r mi nanto ft hi o r e do x i nr e do xs t at e

  A no t he rpat hwa ymus tbeabl et or e duc e t hi o r e do xi n

 Ac kno wl e dg e me nt 

Dr .Ga r yMe r r i l l 

Bi o c he mi s t r y& Bi o p hy s i c sLa b



Dr .Ke vi nAhe r n



Ho war dHughesMedi c alI ns t i t ut e



Unde r gr aduat e ,Re s e ar c h,I nno v a t i o n, Sc ho l ar s hi p& Cr e at i vi t y



www. pr o s us pe ns i o nt r ai ne r . c o m