Descripción: Ejemplos prácticos de ejercicios con TRX
Descripción: Trx training
Official trx manualFull description
Full description
tactical teasingDescrição completa
Full description
Descripción completa
Descripción: Official trx manual
Official trx manual
Osprey PublishingDescription complète
Descripción completa
TRX ForceTacticalKit www. pr os us pens i ont r ai ner .
Thi o r e do x i n
pr o t e i ne s s e nt i alf o rl i f ei nmammal s
ac t sasanant i o x i dantt opr e v e nto x i dat i v e s t r e s s a c t sasane l e c t r o ndo no ri nav ar i e t yo f c e l l ul arr e ac t i o ns r e s t o r e so xi dat i v el yi nac t i v at e de nz yme s t ot he i rr e duc e d& ac t i v es t at e www . pr o s us pe ns i o nt r ai ne r . c o m
Thi o r e do xi ne xi s t si ne i t he rt her e duc e d o ro xi di z e df o r m H
H
S
S
S
S
Trx
Trx
Reduced Thioredoxin
Oxidized Thioredoxin
LEGEND SH = thiol S-S = disulfide
Thi o r e do xi npar t i c i pat e si ndi t hi o l di s ul fidee x c hang er e ac t i o ns S
S
RNR oxidized
H S
H S
reduced Trx
H S
H S
RNR reduced
S
S
oxidized Trx
Thi o r e do x i nr e duc t as e& t hi o r e do x i n www. pr o s us pe ns i o nt r ai ne r . c o m thioredoxin H S NAD!
thioredoxin reductase NADH
H S
Trx
S
S
Trx
ribonucleotide reductase S S RNR
H S
H S
RNR
Hy po t he s i s
Thi o r e do x i nr e duc t as ei sama j o rde t e r mi nanto f t hi o r e do xi nr e do xs t at e
I ft r ue ,t he nde l e t i o no ft hi o r e do xi nr e duc t as e s ho ul ds hi f tt her e do xs t at eoft hi o r e do xi nf r o m pr i mar i l yr e duc e dt opr i mar i l yo xi di z e d
www. pr o s us pe ns i o nt r ai ne r . c o m
Pr o t e i ne l e c t r o pho r e t i cmo bi l i t ys hi f tas s a y( PEMSA) SASASASH SASASH SASASASH SASASASH SASASA- acetone SH SASA"A# DTT SA- 'ash SH "AA SA- "odoaceta$ide TRX r e d
TRX r e d
TRX r e d
"odoacetic acid
TRX o x
SH SH SH SH S S
TRX r e d
Dithiothreitol
TRX o x
SASASASAS S
TRX o x
SASASASASH SH
LEGEND A- = ne%ati&e char%e = Thioredoxin
# = neutral state S = Disulfide
TRX o x
SASASASAS# S#
Pr o t e i ne l e c t r o pho r e t i cmo bi l i t ys hi f tas s a y SH SH SH SH SH SH
Tr x r e d
SH SH SH SH S S
Tr x o x
DTT
E G A a e r u
SH SH SH SH SH SH
"AA("A# "AA
-
) -* -+ -, -
TrxSS
-. !
"A#
-/
TrxSH *
SASASASASASA-
S# S# S# S# S# S#
S# or SAS# or SAS# or SAS# or SAS# or SAS# or SA-
+
,
Re pr e s e nt at i v er e s ul t s 3ild T04e 0 l o n A " A
0 l o n # " A
*
+
,
#utant -
.
1 ,
) ) +
* + )
2 + +
, ) +
e r d d L a
Trxox
$ixed disulfide
Trxred
Tr xoxidized
6
Mi x e d
12 10 14 13 15 17 17 20 21 18
d i s u l fi d e Tr xreduced
7
8
7
9
12 16 21 23 21
27 22 27 23 29 29 30 34 36 35
WT
Mut
Trxox
16%
26%
$ixed disulfide
28%
27%
Trxred
56%
47%
Co nc l us i o n
Thi o r e do x i nr e duc t as ei sno tt hema j o r de t e r mi nanto ft hi o r e do x i nr e do xs t at e
A no t he rpat hwa ymus tbeabl et or e duc e t hi o r e do xi n
Ac kno wl e dg e me nt
Dr .Ga r yMe r r i l l
Bi o c he mi s t r y& Bi o p hy s i c sLa b
Dr .Ke vi nAhe r n
Ho war dHughesMedi c alI ns t i t ut e
Unde r gr aduat e ,Re s e ar c h,I nno v a t i o n, Sc ho l ar s hi p& Cr e at i vi t y