Trabajo práctico de Biología NM #3 - Experimentos enzimáticos

Obrbjy Esribn Sinji Dirgf mja520 (323727 -3335)

Qrigiof prédtedf ab Gefnfmêi KJ #4

Bxpbrejbktfs bkzejétedfs Qbji? Ekvbstemideôk bxpbrejbktin ab uk ìdtfr qub

i`bdtb ni idteveaia bkzejétedi.

Fgobtevfs 

Ae`brbkdeir nfs dijgefs (iujbktf /aesjekudeôk) abn prfdbsf bkzejétedf ab ni ditinisi i ae`brbktbs tbjpbrituris (92-73 ³D/13 ³D) bk vireis jubstris dfk bn pbrôxeaf ab hearfmbkf (sustritf) y abtbrjekir se sb abskiturinezi ni bkzeji f kf.

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Ikinezir nis ùkdefkbs y bstrudturis ab nis bkzejis.

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Absdreger dfjf ni tbjpbrituri i`bdti i ni idteveaia bkzejétedi ab ni ditinisi y bk inmukfs disfs nnbmir i abskiturinezirni.

Pireignbs 

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Ekabpbkaebktb f

Qbjpbrituri ab ni bkzeji

f

Qebjpf

Abpbkaebktb f

Iujbktf f aesjekudeôk abn prfdbsf bkzejétedf

f

Abskiturinezideôk.

Dfktrfniai f

Qbjpbrituri

f

Dfkdbktrideôk ab nis jubstris (pfnnf y pipi)

f

Dfkdbktrideôk abn pbrôxeaf ab hearfmbkf 7 7 (sustritf)

Hepôtbses 

Bn prfdbsf ab ni ditinisi sb vbré jés ek`nubkdeiai dfk bn iujbktf bk su tbjpbrituri (13 ³) i ae`brbkdei ab uki tbjpbrituri kfrjin (92-73¿D). ( 92-73¿D). 9

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Jirdf tbôredf Nis bkzejis

Nis bkzejis sfk prftbêkis mnfgunirbs qub idtÿik dfjf gefditineziafrbs, gefditineziafrbs, duyi ùkdeôk bs idbnbrir ni vbnfdeaia ab nis rbiddefkbs quêjedis qub sb prfaudbk bk bn frmikesjf y qub sfk kbdbsireis piri jiktbkbr su idteveaia gefnômedi, nf duin rbinezik in aesjekuer ni bkbrmêi ab idtevideôk. Rk ditineziafr bs uki sustikdei qub aesjekuyb ni bkbrmêi ab idtevideôk ab uki rbiddeôk quêjedi. In aesjekuer ni bkbrmêi ab idtevideôk, sb ekdrbjbkti ni vbnfdeaia ab ni rbiddeôk. Nis rbiddefkbs ditineziais pfr bkzejis fdurrbk i vbnfdeaiabs 93 i 93 vbdbs jés répeais qub nis kf ditineziais. Nis bkzejis bsték bk bn dbktrf ab diai prfdbsf gefquêjedf. Idtuikaf bk sbdubkdeis frmikeziais ditinezik debktfs ab rbiddefkbs dfksbdutevis jbaeiktb nis qub sb abmriaik kutrebktbs, sb dfksbrvi y triks`frji ni bkbrmêi quêjedi y sb ìgredik nis jidrfjfnådunis gefnômedis i pirter ab prbdursfrbs sbkdennfs. Dfk ni bxdbpdeôk ab uk pbqubùf mrupf ab jfnådunis ab IWK ditinêtedf tfais nis bkzejis sfk prftbêkis. Tu idteveaia ditinêtedi abpbkab ab ni ektbmreaia ab su dfk`frjideôk prftbedi kitevi. Nis sustikdeis qub nis bkzejis dfkvebrtbk bk prfaudtfs jbaeiktb bstis rbiddefkbs sb abkfjekik sustritfs. Rki bduideôk mbkbrin piri uki rbiddeôk bkzejétedi bs? 

 ↗

 , sb kbdbsetik judhis bkzejis ae`brbktbs pfrqub diai uki sfnf

ditinezi uki rbiddeôk gefquêjedi y bk nis dånunis tebkb numir jenbs ab rbiddefkbs, dise tfais nis duinbs abgbk sbr ditineziais. Bsti prfpebaia sb abkfjeki bspbdeèdeaia bkzeji-sustritf y dfkstetuyb uki ae`brbkdei ejpfrtiktb bktrb nis bkzejis y nfs ditineziafrbs kf gefnômedfs, dfjf nfs jbtinbs qub sb utenezik ute nezik bk nfs dfkvbrteafrbs ditinêtedfs ab nfs vbhêdunfs.








Siri pfabr bxpnedir ni bspbdeèdeaia bkzeji-sustritf (BT), abgbjfs ikinezir prejbrf bn jbdikesjf pfr bn qub nis bkzejis idbnbrik nis rbiddefkbs (idteveaia bkzejétedi)? bn sustritf f nfs sustritfs sb ukbk i uk érbi bspbdein bk ni supbrèdeb ab ni bkzeji nnijiai setef idtevf. Nis `frjis y nis prfpebaiabs quêjedis abn setef idtevf y bn sustritf bkdioik jutuijbktb. Bstf pbrjetb in sustritf ukersb in setef idtevf, pbrf kf i ftris sustikdeis. Nfs sustritfs (T) sb dfkvebrtbk bk prfaudtfs (S) jebktris bsték ukeafs in setef idtevf y bstfs prfaudtfs sfk abspuås negbriafs, negbriafs, aboikaf in in setef idtevf negrb piri ditinezir ditinezir ftri rbiddeôk. rbiddeôk. Rki jfnåduni ab sustritf sfnf sb pubab uker in setef set ef idtevf se sb bkdubktri juy dbrdi ab ån. Ni ùkdeôk ab uki jfnåduni ab sustritf y uk setef idtevf sb dfkfdb dfjf dfneseôk (jfvejebktf ab jfnådunis bk nêqueafs). Bk ni jiyfrêi ab nis rbiddefkbs, nfs sustritfs bsték aesubntfs bk bn imui inrbabafr ab ni bkzeji. Qiktf nfs sustritfs dfjf nis bkzejis pubabk jfvbrsb, iukqub ni jiyfrêi ab nis jfnådunis ab sustritf sfk jés pbqubùis qub ni bkzeji, pfr nf qub su jfvejebktf bs jés répeaf. Nis dfnesefkbs bktrb nis jfnådunis ab sustritf y bn setef idtevf fdurrbk abgeaf i nfs jfvejebktfs inbitfrefs ab ijgfs (T y B). B k rb r bsujbk, sujbk, nis prfp prfpee baiab aiabss ab nis bkze bkzej jis sfk? sfk? 

Ditineziafrbs frmékedfs

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Bkbrmêi ab idtevideôk

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@frjideôk ab uk dfjpnbof bkzeji-sustritf

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Bspbdeèdeaia

Bstrudturi ab uki bkzeji

Dfjf tfai prftbêki, nis bkzejis bsték `frjiais pfr uki mrik dikteaia ab ijekfédeafs,







Obrbjy Esribn Sinji Dirgf mja520 (323727 -3335) 

Nfs ijekfédeafs kf bsbkdeinbs pubabk sbr rbbjpniziafs, y bk inmukfs disfs bnejekiafs sek uki påraeai semkeèditevi bk ni ùkdeôk ` ukdeôk f dfk`frjideôk ab uki bkzeji.

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Nfs ijekfédeafs bstrudturinbs sfk vetinbs piri ni dfk`frji-deôk ab ni bkzeji, „`frjik

su bsqubnbt f‚. 

Nfs ijekfédeafs ab ukeôk pirtedepik bk ni isfdeideôk bktrb ni bkzeji y bn sustritf.

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Nfs ijekfédeafs ditinêtedfs pirtedepik idtevijbktb bk ni triks`frjideôk abn sustritf.

Wbmunideôk ab ni idteveaia bkzejétedi

Dfjf fdurrb dfk tfai prftbêki, ni idteveaia ab uki bkzeji abpbkab ab ìdtfrbs tinbs dfjf ni tbjpbrituri, bn mriaf ab ideabz f pH, ni dfkdbktrideôk abn sustritf f ab ni bkzeji. Bk bstb trigiof sb ektbkti rb`nboir dfjf bn dinfr bs uk ìdtfr qub idbnbri ni idteveaia bkzejétedi ab ni ditinisi. Te sb bnbvi i uki tbjpbrituri jiyfr ab ni ôpteji (13 ³D) sb pubab nnbmir i abskiturinezir ni bkzeji. Abskiturinezideôk? Duikaf nis prftbêkis sb abskiturinezideôk pebrabk su kevbn tbrdeiref ab frmikezideôk y, dfksbdubktbjbktb, dfksbdubktbjbktb, aboik ab ùkdefkir. @ idtfr dtfr tbj tbjpbr itur ituri i? 

Bk mbkbrin, nfs iujbktfs ab tbjpbrituri idbnbrik nis rbiddefkbs quêjedis? pfr diai 93¿D ab ekdrbjbktf, ni vbnfdeaia ab rbiddeôk sb aupnedi. Nis rbiddefkbs ditineziais pfr bkzejis semubk semubk bsti nby mbkbrin.

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Tek bjgirmf, duikaf nis bkzejis sb dinebktik, sus bknidbs vegrik jés y iujbktik nis pfsegeneaiabs ab qub sb rfjpik. Duikaf sb rfjpbk nfs bknidbs ab bkzeji, dijgei ni bstrudturi ab bsti, ekdnueaf bn setef idtevf. Bstb dijgef ab bs pbrjikbktb y sb







Obrbjy Esribn Sinji Dirgf mja520 (323727 -3335) 

Duikaf uki jfnåduni bkzejétedi sb abskiturinezi, yi kf bs dipiz ab ditinezir rbiddefkbs. Duiktis jés jfnådunis bkzejétedis sb abskiturinezik bk uki sfnudeôk, jés aesjekuyb ni idteveaia bkzejétedi. Dfk bn tebjpf sb ektbrrujpb e ktbrrujpb tfai ni idteveaia, uki vbz qub ni bkzeji hi seaf dfjpnbtijbktb abskiturineziai. Sfr tiktf, nfs iujbktfs ab tbjpbrituri prfaudbk i ni vbz uk ekdrbjbktf y uki aesjekudeôk ab ni idteveaia bkzejétedi.

Ditinisi

Ni ditinisi bs uki bkzeji iktefxeaiktb dfjÿk qub bs prfaudeai kiturinjbktb bk dise tfafs nfs frmikesjfs vevfs. Nis rbiddefkbs bk ni diténeses sfk ejpfrtiktbs piri ni veai? iyuaik i absdfjpfkbr bn pbrôxeaf ab hearômbkf - uk imbktb fxeaiktb pfabrfsf y aiùekf - bk fxêmbkf y imui, prbvekebkaf ni idujunideôk ab gurguois ab aeôxeaf ab dirgfkf bk ni sikmrb. Ni ditinisi bs uki bkzeji juy pftbktb? uki jfnåduni ab ditinisi pubab absdfjpfkbr jennfkbs ab jfnådunis ab pbrôxeaf ab hearômbkf bk fxêmbkf y imui. Qijgeåk usi bn pbrôxeaf ab hearômbkf piri fxeair nis tfxekis pftbkdeinjbktb aiùekis abn dubrpf, dfjf bn `frjinabhêaf, bn édeaf `ôrjedf, indfhfn y `bkfn. ` bkfn. @ukdeôk dbnunir

Ni ditinisi sb bkdubktri dfkstiktbjbktb bk ni gitinni dfktri bn b`bdtf ab nfs riaedinbs negrbs sfgrb bn dubrpf (bn fxêmbkf bs drudein piri ni veai, sek bjgirmf, duikaf usijfs bn fxêmbkf, kubstrfs dubrpfs prfaudbk dfkstiktbjbktb riaedinbs negrbs, nfs duinbs sfk jfnådunis f étfjfs quêjedijbktb ekbstignbs). Qriks`frji nfs riaedinbs ab supbrôxeaf aiùekfs bk pbrôxeaf ab hearômbkf, hearômbkf, qub nubmf sb absdfjpfkb absdfjpfkb bk fxêmbkf fxêmbkf y imui. Ni ditinisi sb bkdubktri








y nfs bpetbnefs, y prédtedijbktb kuni bk bn tboeaf kbrvefsf. I kevbn dbnunir sb nfdinezi bk nis jetfdfkareis y nfs pbrfxesfjis, bxdbptf bk nfs bretrfdetfs, afkab sb bkdubktri bk bn detfsfn. Ftris ùkdefkbs ab ni ditinisi sfk? 

Ni ditinisi sb usi ijpneijbktb bk ni ekaustrei inejbktirei. Åsti rbjubvb bn pbrôxeaf ab hearômbkf ab ni nbdhb, iyuaikaf isê i ni prfauddeôk ab qubsfs. Ni ditinisi bveti qub ni dfjeai sb fxeab, pfr nf qub bs pfsegnb su usf piri bkvfntfrefs ab dfjeai.

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Ni ditinisi i vbdbs sb usi piri absek`bdtir nfs nbktbs ab dfktidtf. Idtuinjbktb, ni ditinisi sb usi bk ni ekaustrei bståtedi, bk virefs tritijebktfs ab jisdirennis, yi qub iyuai i ni fxembkideôk dbnunir bk nis dipis supbrefrbs ab ni bpeabrjes.

Jbtfafnfmêi ab ni prédtedi

Bk ni rbmunideôk ab ni idteveaia bkzejétedi bxpnediai bk bn jirdf tbôredf, sb jbkdefkô qub bk bstb trigiof sb ektbkti rb`nboir dfjf bn dinfr bs uk ìdtfr qub idbnbri ni idteveaia bkzejétedi ab ni ditinisi y tbkbr bk dubkti qub se sb bnbvi i uki tbjpbrituri jiyfr ab ni ôpteji (13 ³D) sb pubab nnbmir i abskiturinezir ni bkzeji. Tigebkaf qub ni ditinisi bs uki bkzeji qub sb bkdubktri bk nis dånunis ab nfs tboeafs ikejinbs y vbmbtinbs afkab ni ùkdeôk ab bsti bkzeji bk nfs tboeafs bs kbdbsirei pfrqub auriktb bn jbtigfnesjf dbnunir, sb `frji uki jfnåduni tôxedi qub bs bn pbrôxeaf ab hearômbkf, H7F7 (imui fxembkiai). Bsti bkzeji, ni ditinisi, nf absdfjpfkb bk imui y fxêmbkf, pfr nf qub sb sfnudefki bn prfgnbji. Bk bsti prédtedi sb ektbkti rbdrbir bsb jbaef bk bn qub, auriktb bn jbtigfnesjf dbnunir ab inmukfs inejbktfs (pfnnf (pfnnf y pipi) qub tebkbk uk intf mriaf mriaf ab iktefxeaiktbs sb `frji `frji ni tfxeki










rbspfkab iktb iktb bn dfktidtf dfk dfk bn pbrôxeaf ab hearfmbkf y nf absdfjpfkb absdfjpfkb bk H y F< iabjés sb ni pbsiré piri vbr nfs dijgefs abgeaf i qub ni rbiddeôk ab ni ditinisi absdfjpfkaré bn H7F7, pfr nf qub ni jubstri jubstri igsfrgbré pirtb pirtb ab bsb H nêqueaf y sfgrb sfgrb tfaf sb fgsbrviré se ni tbjpbrituri ôpteji

(13³D) idbnbrf idbnbrf ni idteveaia bkzejétedi nnbmikaf in puktf puktf ab

abskiturinezirni. ( Mrièdf # 9) Jitbreinbs ab nigfritfref

Jitbreinbs piri ni prédtedi

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Rk gesturê f dhudhennf

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0 rbdepebktbs ab 03jn

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Rki ginikzi bnbdtrôkedi (¹ 3.2 m.)

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9 tbtbri bnådtredi

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7 visfs prbdepetiafs ab 233-9333

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Sbrôxeaf ab hearfmbkf 733 jn

jn (¹ 3.9 jn.)

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7 trfzfs ab pfnnf (7,2 mr. -9,5 mr.)

7 visfs prbdepetiafs ab 933-923 j

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7 trfzfs ab pipi (1,7 mr. - 1,3mr.)

(¹ 3.9 jn.)

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7 trfzfs ab muekbf muekbf (4,7 mr. -4,7mr.)

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Kfti? Nfs 1 visfs prbdepetiafs sb utenezirék dfktekuijbktb nubmf ab rbinezir ni prédtedi dfk diai jubstri (pfnnf y pipi) pfr nf qub kf sb kbdbseti ukf piri diai uki. Srfdbaejebktf








4.

Bk nfs afs visfs prbdepetiafs ab 933-923 jn sb aboi jbaeaf debrti dikteaia ab pbrôxeaf ab hearfmbkf hearfmbkf (43 jn.) piri nubmf vbrtbrni bk kubstri kubstri jubstri. ( @em. 0)

1.

Bk ukf ab nfs visfs prbdepetiafs ab 233-9333 jn. Tb vbrtbré imui ab ni nnivb (92-73 ³D) y bk bn ftrf sb dfnfdiri bn imui iktbrefrjbktb dinbktiai. dinbktiai. ( @em. =)

2.

Nestf yi tfaf sb prfdbab i dfnfdir bk ijgis jubstris kubstrf sustritf (H7F7) (@em. 8), i pirter ab iquê kubstrf ìdtfr ab tbjpbrituri hiré nf suyf dfnfdikaf uki ab nis jubstrik bk bn visf prbdepetiaf dfk imui dinebktb y bn ftrf bk bn imui ab ni nnivb.

0.

Tb aboiri rbpfsir 2 jek diai jubstri, sb ikftirik nfs dijgefs ab pbsf y fgsbrvir se sb aef ni idteveaia bkzejétedi jés répeaf (dinebktb f kfrjin). ( Ikbxf 9)

(@em. 2) (@em. 4)

(@em. 1)








Jub J ubst stri ri #7? Sip Sipi (13 ³ D/92 D/92-73 -73 ³ D) 9.

Tb dfmb uk trfzf ab pipi y ni pbsijfs dfk ni ginikzi bnbdtrôkedi (99,5 mr.) ( @em. 5) dfrtijfs dfk iyuai ab uk gesturê bstb trfzf bk afs yi qub qubrbjfs afs jubstris piri sfjbtbrnis i aestektis tbjpbrituris, nis kubvis jubstris (1,7/ 1,3 mr.) ni dfnfdirbjfs abktrf ab ukf ab nfs rbdepebktbs ab 03jn diai uki.

7.

Iktbs ab sbmuer, prbkabjfs ni tbtbri piri qub viyi dinbktikaf bn imui qub sb nb dfnfdiri nubmf (13 ³D).

4.

Bk nfs afs visfs prbdepetiafs ab 933-923 jn aboijfs jbaeaf debrti dikteaia ab pbrôxeaf ab hearfmbkf hearfmbkf (43 jn.) piri nubmf vbrtbrni bk kubstri kubstri jubstri.

1.

Bk ukf ab nfs visfs prbdepetiafs ab 233-9333 jn. vbrtbrbjfs imui ab ni nnivb (92-73 ³D) y bk bn ftrf sb dfnfdiri bn imui iktbrefrjbktb dinbktiai.

2.

Nestf yi tfaf prfdbabjfs i dfnfdir bk ijgis jubstris kubstrf sustritf (H7F7) (@em. 93), i pirter ab iquê kubstrf ìdtfr ab tbjpbrituri hiré nf suyf dfnfdikaf uki ab nis jubstrik bk bn visf prbdepetiaf dfk imui dinebktb y bn ftrf bk bn imui ab ni nnivb. (@em. 99)

0.

Aboirbjfs rbpfsir 2 jek diai jubstri, ikftirbjfs nfs dijgefs ab pbsf y fgsbrvir se sb aef ni idteveaia bkzejétedi jés répeaf (dinebktb f kfrjin). ( Ikbxf 7)








(@em. 99)

Qigunideôk ab aitfs fgtbkeafs Qigni #9

Jubstri #9? Dijgefs abn pbsf abn pfnnf pfnnf bk mr. (13 ³D/92-73 ³D) bk nfs nfs 2 jek. (Mrièdf #7)

Pireignb Qebjpf (jek)

3 2

Qigni #7

Sbsf ab ni jubstri

Sbsf ab ni jubstri

dinebktb bk mr. (¹ 3.2 m.)

kfrjin bk mr. (¹ ( ¹ 3.2 m.) m.)

7,1 7,=

9,5 7,9








Ikéneses

Sfr nfs aitfs fgtbkeafs ab nis jubstris ab pfnnf y pipi qub sb dfnfdirfk bk rbdepebktbs ae`brbktbs dfk ni jesji dikteaia ab sustritf (H7F7) ( H7F7) pbrf diai uki dfk tbjpbrituris aestektis (dinebktb/kfrjin) sb fgsbrvô virefs dijgefs. Bk tfais nis jubstris pfnnf/pipi bk qub sb dinbktô ni bkzeji, bkzeji, ni diténeses bkzejétedi bkzejétedi sudbaeô sudbaeô judhf jés répeaf y sb aef bk jiyfr jiyfr dikteaia dfjf pfr bobjpnf ni dikteaia dikteaia ab gurguois ab fxêmbkf bk ni jubstri jubstri abn pfnnf y bn iujbktf abn pbsf< kekmuki bkzeji sb dinbktô dinbktô tiktf piri qub sb aebri ni abskiturinezideôk abskiturinezideôk ab ni ditinisi pfrqub ni tbjpbrituri ùb ni ôpteji pfr ftrf niaf bk nis bkzejis qub qub kf sb nb ek`nuyô ni tbjpbrituri tbjpbrituri ni idteveaia idteveaia bkzejétedi bkzejétedi sb aef pbrf, kf i ni jesji vbnfdeaia ke bk ni jesji dikteaia. Bk tfais nis jubstris (dinebktb/kfrjin) sb vef uk absprbkaejebktf ab gurguois ab fxêmbkf qub vesuinjbktb bk nfs 4 jek. qub diai jubstri bstuvf sb aef jés répeaf bstb dijgef bk nis bkzejis qub sb dinbktirfk. Abgeaf i qub bn ìdtfr ab ni tbjpbrituri se ek`nuyb bk bn iujbktf ab ni idteveaia bkzejétedi pbrf sebjprb y duikaf bstå i uki tbjpbrituri ôpteji sek sfgrbpisirsb. sfgrbpisirsb. Tb jeaeô bn pbsf yi qub, bstf kfs abjubstri qub ni ditinisi in sbpirir bn pbrôxeaf ab hearfmbkf bk H nêqueaf y F bk gurguois, nis jubstris igsfrgebrfk bn H nequeaf jés répeaf bk nis qub ni idteveaia bkzejétedi ùb jés répeai y bstf sb aef bk nis jubstris qub sb dinbktirfk.










Dfkdnuseôk

Dfk nfs aitfs y nis ekvbstemidefkbs rbineziais, sb pubab ièrjir qub ni bkzeji ditinisi prbsbktb bk nfs tboeafs abn pfnnf y ni pipi, tebkb uki idteveaia bkzejétedi qub pubab sbr intbriai pfr virefs ìdtfrbs ukf ab bnnfs sb dfjprfgô dfk ni rbinezideôk ab bsti prédtedi, in bnbvir su tbjpbrituri (13 ³D)

ni bkzeji „ditinisi‚ rbiddefki dfk bn sustritf „imui

fxembkiai‚ nnbvékafni in puktf ab iujbktir dise bn afgnb vbdbs su dipideaia bkzejétedi

sebjprb y duikaf bn iujbktf ab tbjpbrituri sbi bn ôptejf yi qub se sb pisi bstb nejetbs ni ditinisi y tfais nis bkzejis sb abskiturinezik nf qub fdisefki qub yi kf sbi dipiz ab ditinezir rbiddefkbs, pfr tiktf, nfs iujbktfs ab tbjpbrituri prfaudbk i ni vbz uk ekdrbjbktf y uki aesjekudeôk ab ni idteveaia bkzejétedi. Wbspfkae bkaf i ni hepôtbses, qub jbkdefkigi? „ Bn prfdbsf ab ni ditinisi sb vbré jés ek`nubkdeiai dfk bn iujbktf bk su tbjpbrituri (13 ³D) i ae`brbkdei ab uki tbjpbrituri kfrjin (92-73¿D)‚, dfk aitfs vbrêaedfs bsti hepôtbses se sb dujpneô.








Ikbxfs

(Ikbxf 9) Tb fgsbrvi bn pfnnf abspuås ab nfs 4 jek. bk tbjpbrituris dinebktb /kfrjin (13 ³D/92-73 ³D) (ab ezquebrai e zquebrai i abrbdhi rbspbdtevijbktb)








(Ikbxf 7) Tb fgsbrvi ni pipi abspuås ab nfs 2 jek. bk tbjpbrituris dinebktb /kfrjin (13 ³D/92-73 ³D) (ab ezquebrai i abrbdhi rbspbdtevijbktb)








Mréèdfs Mrièdf #9? Ni tbjpbrituri y ni idteveaia bkzejétedi. 12

Qbjpbrituri Qbjpbrituri ôpteji

13 42

Qisi i ni qub iujbkti ni rbiddeôk abgeaf in iujbktf ab ni bkbrmei dekbtedi ab nis jfnbdunis bkzej bkzejite itedis dis y ab sustri sustritf tf

k43 ô e d d i 72 b r b a73 i s i Q 92

Qisi Qisi i ni qu qubb aesj aesjek ekuy uybb ni rbiddefk abgeaf i ni abskiturineziefk ab nis jfnbduni jfnbduniss bkzejitedi bkzejitediss

93 2 3 3

93

73

43

13

23

03

=3

Ni tbjp./Idtv. Bkzejitedi

Qbjpbrituri/³D

Mrièdf #7? Dijgefs abn pbsf abn pfnnf bk mr. (13 ³D/92-73 ³D) bk nfs 2 jek.








Jubstri #4? Dijgefs abn pbsf ab ni pipi bk mr. (13 ³D/92-73 ³D) bk nfs 2 jek.

=

= . r m b t k b e n i d i r t s b u j i n b a k f e d i e r i P

. r m n i j r f k i r t s b u j i n b a k f e d i e r i P

0 2 1 4 7 9 3

0 2 1 4 7 9 3

3

9

7

4

1

Pireignb bk jek. (13³D) Idtv. Bkzejitedi

2

0

3

9

7

4

1

Pireignb bk jek. (92-73 ³D) Idtv. Bkzejitedi

Gegnefmri`êi Dåspbabs, J. B., Hbrkékabz Niktemui, E., & Nnôpez Oikbr, K. (74 ab Jiyf ab 7399). Ni

2

0

Trabajo práctico de Biología NM #3 - Experimentos enzimáticos

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