LAPORAN PRAKTIKUM BIOFARMASETIKA
STUDI ABSORPSI OBAT SECARA INVITRO Selasa, 4 Oktober 2016 Kelo!ok II Selasa, P"k"l 0#$00 % 10$00 &IB Naa 1$ '"(ae)* S$ S$ 2$ &*r( &*r(aa +$ T$ T$ S* S*o ob* b*((.$ F*tr F*tr*a *a /$ A A" 4$ Astr Astr** S"l S"las astr tr** $ asb M$ '$ '$ 6$ &*()a ()a Rat( Rat(aa P$ #$ Fe* A$ 3$ Me-a Me-a Tr*(o *(oaa 5$ R*o Sa! Sa!" "tra 10$ N"r F*tr*at"77ak F*tr*at"77ak*a *a
NPM 2601101.0051 2601101.0052 2601101.005. 2601101.0054 2601101.005 2601101.0056 2601101.005# 2601101.0053 2601101.0055 2601101.0100
LABORATORIUM BIOFARMASETIKA FAKULTAS FARMASI UNI8ERSITAS PA9'A9'ARAN 2016
I.
Tujuan Percobaan
Me!erla:ar* !e(-ar" ! tera)a! absor!s* obat elal"* sal"ra( !e(er(aa( seara *( *tro$ II.
Prinsip Percobaan 1$ Absor!s* Absor!s* ata" !e(era!a( 7at akt*; a)ala !er!*()aa( obat )ar* !roses
a)*(*stras* ke )ala s*rk"las* )ara$ Kee!ata( )a( :"la a()*absor!s* ber-a(t"(- !a)a r"te a)*(*stras*, te!at !eber*a( obat a)ala sal"ra( er(a <"l"t sa!a* )e(-a( ret"=, k"l*t, !ar", otot, )a( la*(>la*(, ;or"las* )a( s*;at ;*s*kok**a )ar* obat terseb"t, ;aktor ;*s*olo-* a(- )a!at e!e(-ar"* !roses absor!s* obat
"la$9era:at *o(*sas* ter-a(t"(- !a)a ! lar"ta( )a( !Ka obat se!ert* terl*at !a)a !ersaaa( asselbal asselba U(t"k asa Fraksi obat yangterionkan pH = pKa+ log Fraksi obat yang tak terionkan
pH = pKa−log
e()erso(>
U(t"k basa
Fraksi obat yangterionkan Fraksi obat yangtak terionkan <&atso(, 200#=
4$ Kee!ata( Tra(s!ort obat Pereab*l*tas ebra(e b*olo-* tera)a! s"at" obat )a!at )*-abarka( ole
koe;*s*e(
!art*s*(a
)e(-a( kee!ata(
)a(
e!"(a*
"b"(-a(
tra(s!ort ata" kee!ata( absor!s*(a,
l*(ear
)*(ataka(
)e(-a( !ersaaa( ? δQb = Pm . Cg δt
)ala a*r aka( ber!e(etras* ke )ala sel )e(-a( ba(t"a( olek"l ata" ele@at* ka(al k"s"s
Teori Dasar
Absor!s* a)ala !er!*()aa( obat )ar* !roses a)*(*stras* ke )ala s*rk"las* )ara$ Kee!ata( )a( :"la a(- )*absor!s* ber-a(t"(- !a)a r"te a)*(*stras*, te!at !eber*a( obat a)ala sal"ra( er(a <"l"t sa!a* )e(-a( ret"=, k"l*t, !ar", otot, )a( la*(>la*(, ;or"las* )a( s*;at ;*s*kok**a )ar* obat terseb"t, ;aktor ;*s*olo-* a(- )a!at e!e(-ar"* !roses absor!s* obat
+abar 1$ +abar ko(se(tras* obat )ala ser" )e(-a( @akt" "(t"k a)*(*stras* obat seara oral )a( *(trae(a$ Ket*ka obat )*ber*ka( seara *(trae(a aka !roses absor!s* t*)ak )*b"t"ka( kare(a obat a(- )*ber*ka( la(-s"(- eas"k*( s*ste s*rk"las* )ara$ Sel"r" )os*s obat a(- )*a)*(*stras*ka( aka( bera)a !a)a s*s* drug action. Se)a(-ka( "(t"k !eber*a( obat sela*( *(trae(a, keterse)*aa((a aka( leb* re()a- kare(a e(-ala* absor!s* a(- t*)ak se!"r(a$ Ket*ka *(* ter:a)*,
)os*s obat a(- )*a)*(*stras*ka( aka( e(-ala* !e("r"(a( "(t"k bera)a !a)a s*s* drug action
+abar 2$ 9*;"s* !as*; )a( tra(s!ort akt*; ;aktor la*( a(- )a!at e!e(-ar"* absor!s* obat a*t" ? 1$ N*la* !Ka )ar* obat$ Obat a(- bers*;at asa lea aka( a!" ele@at* ebra( !a)a ! a(- leb* re()a )*ba()*(-ka( !Ka )a( obat a(- bers*;at basa lea a!" ele@at* ebra( !a)a ! )*atas (*la* !Ka$ 2$ Kee!ata( )*sol"s* obat$ Bebera!a obat t*)ak lar"t !a)a kea)aa( asa )* lab"(- teta!* lar"t )ala kea)aa( basa )* sal"ra( er(a$ .$ Kestab*la( obat 4$ Ko(se(tras* obat $ Al*ra( )ara$ A!ab*la al*ra( )ara e(*(-kat aka absor!s* obat aka( IV.
e(*(-kat Alat>alat be)a > Beaker -lass > +elas "k"r > Lab" "k"r > S!ektro;otoeter > Sr*(-e
> > > I8$2$ > > > > > > V.
Tab"(- ra(e )a( @*lso( a(- )*o)*;*kas* Teroeter 8*al Baa( Asa Sal*s*lat /a*ra( lab"(- b"ata( ta(!a !e!s*( ! 1,2 /a*ra( "s"s b"ata( ta(!a !e!s*( ! #,6 Eter e@a( Perobaa(
Prosedur $1 Peb"ata( k"ra bak" 10 - asa sal*s*lat sta()ar )*tabaka( l *(--a 100 L$
Ke")*a( )*b"at e(:a)* ko(se(tras* 0 !!, 100 !!, 200 !!, )a( 400 !!$ Mas*(->as*(- lar"ta( )*as"kka( ke )ala k"et )a( )*as"kka( ke alat s!ektro;otoeter ">*s )* !a(:a(- -eloba(- 25# ($ Lal" )*"k"r as*(->as*(- absorba(s*(a$ Setela *t" )*lak"ka( !er*t"(-a( )a( )*b"at k"ra kal*bras*(a$ $2 Pe(e(t"a( Absor!s* !a)a "s"s al"s t*k"s e@a( !erobaa( )*!"asaka( selaa 20>24 :a, teta!* )*ber* *(" a*r asak$ Lal" t*k"s )*b"(" )e(-a( eter, ke")*a( )*b"ka !er"t(a )* se!a(:a(- l*(ea e)*a(a )a( "s"s )*kel"arka($ Setela *t", "s"s se!a(:a(- 1 )* ba@a !lor"s )*b"a(- )a( 22 )* ba@a(a )*!oto(- "(t"k !erobaa($ Lal" "s"s )*ba-* )"a saa !a(:a(- )a( )*bers*ka($ Ba-*a( a(al )*-"(aka( seba-a* ko(trol se)a(-ka( ":"(- a(al )ar* !oto(-a( "s"s terseb"t )**kat )e(-a( be(a(-$ Ke")*a( )e(-a( e(--"(aka( l*)*, "s"s terseb"t )*bal*k se*(--a ba-*a( "kosa terletak )* l"ar$ Us"s )**s* )e(-a( lar"ta( Na/l 0,5 bC seba(ak l$ Lal" "s"s a(- s")a )**s* Na/l )a( )**kat, )*as"kka( ke )ala tab"(- a( ber*s* a*ra( "kosal ! 1,2 seba(ak 10 l <a(- e(-a()"(- asa sal*s*lat= )a( !a)a s"" .#o/$ Perlak"a( *(* )*"la(-* )e(-a( tab"(- a( ber*s* a*ra( "kosal ! #,4 <a(- e(-a()"(- asa sal*s*lat= )a( "(t"k ko(trol e(--"(aka( a*ra( "kosal ! 1,2 )a( ! #,4 :"-a teta!* ta(!a baa( obat$ Obat a(- aka( )*":* )ala !erobaa( *(* a)ala asa
sal*s*lat$ Selaa !erobaa( berla(-s"(-, sel"r" ba-*a( "s"s )*:a-a a-ar )a!at tere()a )ala a*ra( "kosal )a( selal" )*al*r* -as oks*-e( )e(-a( kee!ata( k*ra>k*ra 100 -eleb"(- !er e(*t$ U(t"k lar"ta( ":*
Data Pen!a"atan dan asil 6$1 S"asa(a a*ra( "s"s b"ata( ta(!a !e!s*(
Absorbansi
0
0$411
100
0$20#
200
0$60.
400
0$#5.
Kurva Baku Asam Salisilat (Dalam Basa) 1
Absorbansi
f(x) = 0x + 0.39 R² = 0.98
0.5 0 0
50 100 150 200 250 300 350 400 450
Konsentrasi (ppm)
Persaaa( l*(*er )ar* k"ra bak" a(- )*!erole a)ala ? y =0.001 x + 0.3189
2$ Pe(-"k"ra( Ko(se(tras* Sa!el Ko(se(tras* sa!el )**t"(¿ 0.001 x + 0.3189
elal"*
!ersaaa(
-ar*s
l*(*er
, )e(-a( eas"kka( (*la* absorba(s* sa!el ke
)ala <= se*(--a )*!erole
/
<:"laobat=
k""lat*;
Absorba(s*
=
0$.354
!! 0$424
0$6.31
0$6.31
10
0$41#6
23$6#.4
4.$0101
4.$6432
.0
0$2#3
1.3$#54
203$151#
21$3.55
60
0$6035
215$542.
.25$51.4
31$#..
.$ Pe(e(t"a( Pereab*l*tas, lag time, )a( Ka
Kurva Waktu terhadap Q kumulatif 700 600 f(x) = 10.63x - 59.44 R² = 1
500 400
Q kumulatif 300 200 100 0 0
10
20
30
40
50
60
Waktu (menit)
9*!erole !ersaaa(l*(*er ? y =10.62 x −59.44 •
•
Kee!ata( Absorba(s* Ka (*la* slope 10$62 Pereab*l*tas
Ka 10.62 = =7.6878 P Cg 1,3814
•
Ketera(-a(? /- a)ala ko(se(tras* obat )*l"ar ebra(e "s"s Lag time Lag time a)ala ar-a !er!oto(-a( -ar*s )e(-a( s"b" D <@akt"= 0 0 10$62D % 5$44 59.44 =5.597 x = 10.62
6$2 S"asa(a a*ra( lab"(- b"ata( ta(!a !e!s*(
Absorbansi
$pp"% 0 60 #0 30
0$461 0$03 0$2.6 0$620
70
Kurva Baku Asam 0.6 f(x) = 0x + 0.31 R² = 0.98
0.5 0.4
Absorbansi
0.3 0.2 0.1 0 45
50
55
60
65
70
75
80
85
Konsentrasi (ppm)
Persaaa(
l*(*er
)ar*k"rabak"
a(-
)*!erolea)ala
?
y =0.0031 x + 0.3136 $ Pe(-"k"ra( Ko(se(tras* Sa!el Ko(se(tras* sa!el )**t"(¿ 0.0031 x + 0.3136
elal"*
!ersaaa(
-ar*s
l*(*er
, )e(-a( eas"kka( (*la* absorba(s* sa!el ke
)ala <= se*(--a )*!erole
Absorba( s*
/
<:"laoba t= 0$#23
0$#23
k""lat*;
=
0$.1.5
*= !! 0$43.5
10
0$..13
25$.43
44$0.2.
44$#31
.0
0$.553
1.5$0.2.
203$434
2.$.06
60
0$421
22.$.3#1
..$0306
33$.3#1
6$ Pe(e(t"a( Pereab*l*tas, lag time, )a( Ka
Kurva Waktu terhadap Qkumulatif 700 600 f(x) = 10.74x - 60.06 R² = 1
500 400
Qkumulatif 300 200 100 0 0
10
20
30
40
50
60
70
Waktu (menit)
9*!erole !ersaaa( l*(*er ? y =10.737 x −60.058 •
•
Kee!ata( Absorba(s* Ka (*la* slope 10$#.# Pereab*l*tas
•
VII.
Ka 10.737 = =7.772 Cg 1,3814
Ketera(-a(? /- a)ala ko(se(tras* obat )*l"ar ebra(e "s"s Lag time Lag time a)ala ar-a !er!oto(-a( -ar*s )e(-a( s"b" D <@akt"= 0 0 10$#.#D % 60$03 60.058 =5.59 x = 4 10.737
Pe"baasan
Pa)a !erobaa( kal* *(* )*lak"ka( st")* absorbs* obat seara *( *tro "(t"k el*at !e(-ar" ! tera)a! absorbs* obat$ St")* seara *( *tro se()*r* art*a !erobaa( )*lak"ka( )* l"ar t"b" akl"k *)"!C e@a( ":* se*(--a or-a( a()*-"(aka( )ala !erobaa( bebas )ar* ;"(-s* re-"las* t"b" !a)a ""(a$ St")* )*lak"ka( )e(-a( el*at !e(-ar" ! tera)a! kelar"ta( obat se*(--a
)a!at e(-abarka( )ar* kelar"ta( obat !a)a ! ;*s*olo-*s t"b", ba*k asa ata" !"( basa$ Pa)a ""(a, obat a(- bers*;at basa aka( ebe(t"k -ara a(lar"t !a)a ko()*s* e)*a asa, berlak" !"( sebal*k(a$ 9e(-a( )e*k*a(, s*;at ;*s*kok**a aka( e!e(-ar"* kee!ata( absorbs* s"at" obat$ Sala sat" )*a(tara(a a)ala )era:at *o(*sas* saat obat bera)a!a( )e(-a( ebra($ Besar(a ! )a( !Ka )ar* s"at" obat aka( e!e(-ar"* )era:at *o(*sas* obat terseb"t$ Pa)a !erobaa( kal* *(*, e@a( ":* a(- )*-"(aka( a)ala seekor t*k"s :a(ta( a(- tela )*!"asaka( selaa 20>24 :a (a"( )*ber* *("$ al *(* )*lak"ka( "(t"k e(:a-a a-ar sal"ra( !e(er(aa((a koso(- se*(--a aka( e!er")a !e(-ab*la( )a( !ebers*a( "s"s$ T*k"s
:a(ta(
)*-"(aka(
kare(a )a!at e(-"ra(-* a)a(a barr*er a(- e!e(-ar"* absorbs* obat ak*bat akt**tas oro($ T*k"s ":* )*b*"s total )e(-a( e(--"(aka( a(ates*a a*t" eter )*)ala seb"a aber$ Peb*"sa( *(* )*lak"ka( a-ar e*(*al*sas* ata" e(*a)aka( !e()er*taa( !a)a e@a( ":* ses"a* )e(-a( IA/U/
Setela t*k"s )*b*"s )a( )*!ast*ka( ba@a :a(t"(-(a t*)ak ber)etak la-*, aka )*lak"ka( !ebe)aa($ T*k"s )*!os*s*ka( !a)a !a!a( be)a e(--"(aka :ar" !e(t"l )a( )*!ast*ka( ba@a t"b" t*k"s s")a terletak )e(-a( ba*k se*(--a ")a "(t"k )*lak"ka( !ebe)aa($ Pebe)aa( )*"la* )ar* ba-*a( !er"t ata" "ter"s )e(-a( e(--"(aka( -"(t*(- be(-kok$ M"la>"la )*ab*l ba-*a( k"l*t(a sa:a )e(-a( e(--"(aka( !*sa", k"l*t(a )*s*s*ka($ Sela(:"t(a )*ab*l "s"s )ar* ba-*a( !er"t )a( )*!a(:a(-ka(C)*l"r"ska( Us"s se!a(:a(- 1 )*ba@a !lor"s )*b"a(- )a( 20 )*ba@a(a )*!oto(- "(t"k !erobaa($"s"s ke")*a( )*"* )* a*r bers* )e(-a( ara )*bal*kka(C"s"s terbal*k$ Us"s a(- tela )*"* ta)* )*as"kka( ke )ala *al ber*s* Na/l ;*s*olo-*s$ Saa al(a )e(-a( k"l*t t*k"s sebel"(a )*bers*ka( )a( )*"k"r )ar* se-ala b"l"$ al *(* bert":"a( "(t"k e!ertaa(ka( ! "s"s terseb"t saa se!ert* ! t"b" k*ta$ Perobaa( *(* e(--"(aka( "s"s al"s kare(a ba(ak e*l*k* *l*$ 8*l* "s"s a)ala be()a>be()a ke*l se!ert* be(a(- a(- ela!*s* "s"s a-ar )a!at seara e;ekt*; e(*(-katka( l"as !er"kaa( )*()*(- "s"s se*(--a e(*(-katka( !e(era!a( ("tr*s* aka(a($ 9e(-a( l"as !er"kaa( a(- besar, aka e"(-k*(ka( :*ka !roses absor!s* ber:al a( )e(-a( e!at$ Ke")*a( )*lak"ka( !eb"ata( k"ra bak" asa sal*s*lat )e(-a( eb"at lar"ta( asetosal )e(-a( ar*as* e!at ko(se(tras*$ 8ar*as* terseb"t )*lak"ka( tera)a! lar"ta( stok asa aset*l sal*s*lat 00 !! a(- )*b"at )e(-a( ara elar"tka((a )ala eta(ol$ A)a!"( ko(se(tras* a(- )*b"at )* a(tara(a, 0, 60 #0, 30, 50 !!$ Mas*(->as*(- lar"ta( )e(-a( berba-a* ko(se(tras* terseb"t )*tabaka( /l seba-a* !e()a!ar a-ar e(*(-katka( ! asa, lal" )*"k"r besar absorba(s* )e(-a( s!ektro;otoeter U8>8*s$ Pe(-"k"ra( )*lak"ka( !a)a !a(:a(- -eloba(- aks*", kare(a a(--a!a((a a)ala !a)a !a(:a(-eloba(- terseb"t aka( e(a!*lka( (*la* absorba(s* aks*" se*(--a ke!ekaa( e(*(-kat$ Sela*( *t", :*ka )*lak"ka( !e(-"la(-a( !e(-"k"ra( aka kesalaa( a(- )*sebabka( !easa(-a( "la(- !a(:a(- -eloba(- aka( )**(*al*s*r ket*ka a(- )*-"(aka( a)ala !a(:a(- -eloba(- aks*" <-ol*b, 200#=$ Pa(:a(- -eloba(- aks*al a(- )*)a!at )ar* !erobaa( sebesar 253 ($ Setela l*a lar"ta( )e(-a( ar*as* ko(se(tras* )*"k"r absorba(s*(a,
aka )*b"at k"ra "b"(-a( a(tara absorba(s* rata lar"ta( bak" s"asa(a asa )*)a!at, a*t" !a)a ko(se(tras* 0 !! sebesar 0,461G !a)a ko(se(tras* 60 !! sebesar 0,03G !a)a ko(se(tras* #0 !! sebesar 0,2.6G )a( !a)a ko(se(tras" 30 !! sebesar 0,620$ Ber)asarka( )ata absorba(s* terseb"t )*)a!at !ersaaa( 0,00.1D H 0,.1.3 )e(-a( (*la* re-res* e(a!a* 0,530$ Persaaa( terseb"t ke")*a( aka( )*-"(aka( saat e(-*t"(- ko(se(tras* sa!el )ala s"asa(a asa$ N*la* re-res* a(- e()ekat* (*la* 1 seak*( ba*k art*(a )ar* !ersaaa( terseb"t e(-as*lka( -ar*s l*(*er a(- ba*k$ 9e(-a( )e*k*a( !ersaaa( -ar*s )a!at )*-"(aka($ Perobaa( !a)a "s"s al"s *(* e(--"(aka( eto)e "s"s terbal*k )e(-a( e(--"(aka( Tab"(- /ra(e )a( &*lso( a(- )*o)*;*kas*$ Meto)e "s"s terbal*k er"!aka( eto)e a(- )*lak"ka( )e(-a( ara ebal*k "s"s se*(--a ba-*a( )ala "s"s a(- e(-a()"(- *kro*l* bera)a )* l"ar$ Mebra(e "kosa aka( teta! e!erole oks*-e( se*(--a !roses absor!s* a(- ter:a)* e(er"!a* !roses absor!s* )* t"b"$ Us"s a(- tela )*!erole ke")*a( ba-*a( a(al(a )*-"(aka( seba-a* ko(trol$ U:"(- a(al )ar* !oto(-a( "s"s terseb"t )**kat )e(-a( be(a(-, ke")*a( "s"s terseb"t )*bal*k )e(-a( ba-*a( "kosa bera)a )* l"ar )a( )**katka( !a)a !*!a -elas seara ert*kal )e(-a( e(--"(aka( be(a(-$ bata(-elas a(- )*-"(aka( ber)*aeter 2 $ Pa)a ba-*a( "kosa *(*la ter:a)* !e(era!a( ole *ll* se*(--a )*"saaka( sese)*k*t "(-k*( ko(tak )e(-a( "kosa$ Ka("la )*as"kka( ke ":"(- oral )ar* "s"s a(- bel" ter*kat$ Us"s )*"k"r )e(-a( !a(:a(- e;ekt*; # a(- sebel"(a )**s* )e(-a( a*ra( serosal 1,4 l$ /a*ra( serosal *(* ter)*r* )ar* lar"ta( (atr*" klor*)a
)*ber*ka( elal"* !*!a la*(, a-ar sel>sel )ala "s"s teta! *)"! se*(--a !e(-aata( )a!at )*lak"ka( )e(-a( ba*k$ Pa)a e(*t ke>, ke>10, ke>.0, )a( ke> 60 lar"ta( Na/l ;*s*olo-*s !a)a "s"s a(- )**kat )*ab*l as*(->as*(- !a)a @a)a *al ter!*sa seba(ak 1, L, lal" set*a! !e(-ab*la( )*lak"ka( !er-a(t*a( lar"ta( Na/l ;*s*olo-*s bar" se:"la a(- )*ab*l$ Pe(-ab*la( lar"ta( Na/l ;*s*olo-*s !a)a @akt" terte(t" terseb"t )*lak"ka( "(t"k e(-eta"* ka)ar asa aset*l sal*s*lat a(- terabsor!s* elal"* ;*l*a "s"s )a( as"k ke )ala lar"ta(
serta e(-eta"* absor!s* o!t*al )ar* asa aset*l sal*s*lat !a)a !
terte(t" ses"a* or-a( t"b" a("s*a$ Setela )*ab*l se:"la sa!el !a)a re(ta(- @akt" terte(t" <e(*t ke>, ke>10, ke>.0, )a( ke>60= !a)a ! asa, aka se:"la sa!el terseb"t )*a(al*sa e(--"(aka( s!ektro;otoetr* U8 a-ar e(-eta"* ko(setras* asetosal selaa !roses absor!s* )ala "s"s !a)a ko()*s* ! asa$ A)a!"( !e(-"k"ra( !a)a !a(:a(- -eloba(- a(- tela )*)asarka( !a)a be(t"k k"ra absorba(s* tera)a! ko(se(tras* ee("* "k" Labert>Beer$ Sebel" )*a(al*sa e(--"(aka( s!ektro;otoeter U8, as*(->as*(- sa!el )*as"kka( ke )ala a!"ra( bar*" *)roks*)a 0,. N )a( se(-s"l;at $ F"(-s* )ar* ke)"a 7at terseb"t a)ala "(t"k e(-ekstraks* asa aset*l sal*s*lat a(- ter)a!at !a)a sa!el serta e*saka( se(a@a>se(a@a !e(-otor la*((a a(- "(-k*( aka( e(-"ba as*l !e(-aata($ Bar*" *)roks*)a )*b"at )e(-a( elar"tka( 4,#.2 -ra )ala a*r !a(as seba(ak 100 L, se)a(-ka( se(-s"l;at )*b"at )e(-a( elar"tka( seba(ak -ra )ala 100 L a*r$ '"la bar*" *)roks*)a )a( se(-s"l;at a(- )*lar"tka( terseb"t )*)a!at )ar* !er*t"(-a( (oral*tas )a( !rese(tase$ Pe(--"(aa( bar*" *)roks*)a )a( se(-s"l;at a(- )*tabaka( ke )ala sa!el a)ala as*(->as*(- seba(ak 2 L, lal" sa!el seba(ak 1 L$ /a!"ra( seba(ak L *(* ke")*a( )*se(tr*;"-as* a-ar e*saka( :*ka ter)a!at e()a!a( ata" 7at>7at tak tera!"r$ as*l se(tr*;"-as* )*ab*l ba-*a( ;*ltrat, a*t" ba-*a( :er(* )ar* as*l se(tr*;"-as*$ F*ltrat terseb"t ke")*a( s*a! )*"k"r !a)a s!ektro;otoeter a(- s")a )*>re;ere(e !a)a !a(:a(- -eloba(ses"a* e(--"(aka( bla(ko ! 1,2$ Maka, aka( a)a 4 sa!el !a)a ! asa, a*t" sa!el e(*t ke>, ke>10, ke>.0, )a( ke>60=$
Ber)asarka( as*l !e(-aata(, absorba(s* a(- )*)a!at ses"a* )e(-a( ka*)a "k" Labert Beer, a*t" absorba(s* a(- ter"k"r !a)a re(ta(- 0,2 % 0,4$ Ber)asarka( )ata :"-a )*)a!at (*la* kee!ata( absorbs*, (*la* !ereab*l*tas, ko(se(tras* obat )* l"ar ebra( "s"s, serta la- t*e$ La- t*e a)ala !e("()aa( @akt" absorbs* sebel" !er"laa( absorbs* obat or)e !ertaa ata" @akt" a(- )*b"t"ka( obat "(t"k )*absorbs* e(eb"s ebra($ T":"a( )ar* !e(e(t"a( la- t*e a)ala "(t"k e(-eta"* !a)a e(*t kebera!a obat "la* )*absorbs*, ak*( laa la- t*e aka obat seak*( laa "(t"k )*absorbs*$ Me("r"t !ersaaa( a()erso(%asselba, sear"s(a asa sal*s*lat !a)a ! 1,2
!ereab*l*tas
)**t"(- "(t"k
e(-eta"*
besar(a
!ereab*l*tas ebra( asa sal*s*lat tera)a! ebra( !a)a ! l*(-k"(-a( a(- berbe)a$ N*la* P berba()*(- l"r"s )e(-a( slo!e )a( kare(a (*la* /- teta! aka P ter-a(t"(- slo!e$ Seak*( besar (*la* P aka !ereab*l*tas ebra( tera)a! obat :"-a seak*( besar se*(--a olek"l obat a- terabsorbs* ole ebra( seak*( besar$ '*ka slo!e seak*( besar aka P :"-a seak*( besar$ Seak*( besar ar-a P aka !ereab*l*tas ebra( tera)a! obat seak*( besar se*(--a seak*( ba(ak olek"l obat a(- b*sa ele@at* ebra($ 'a)*, :*ka leb* bers*;at !ereabel aka
olek"l asa sal*s*lat seak*( ba(ak a(-
terabsorbs*$ Keteta!a( !e(etras* a(- t*(--* bel" te(t" ba*k, b*sa :"-a e(-*()*kas*ka( a)a(a ker"saka( "s"s a!a la-*, "s"s er"!aka( or-a( a(sa(-at ra!"$ Ta!* :*ka *()eks )e;*as*(a
e()ekat* berart* !e(eta!a(
!e(etras*(a ba*k$ 9ar* as*l !erobaa( )*)a!atka( !ersaaa( a(tara @akt"
>60$03 , se)a(-ka( !ersaaa( a(tara @akt" 5,44$ Persaaa( *(* )*-"(aka( "(t"k e(e(t"ka( la- t*e$ La- t*e !a)a lab"(- a*t" selaa ,54 e(*t )a( la- t*e !a)a "s"s a*t" selaa ,5# e(*t$ 9ata terseb"t e("(:"kka( ba@a absor!s* obat )* lab"(- leb* e!at )*ba()*(-ka( )* "s"s$ '*ka )*ba()*(-ka( )e(-a( !a)a ko()*s* basa, kee!at !araeter terseb"t e("(:"kka( asa sal*s*lat leb* ba*k se)*k*t absor!s* )* ko()*s* asa$ al terseb"t ses"a* )e(-a( teor* a(- s")a )*:elaska( )* atas$ 9e(-a( )e*k*a(, seba-a* ;aras*s al *t" )a!at )*:a)*ka( seba-a* a"a( )ala e!ro)"ks* s"at" obat se*(--a akt**tas ;arakok*(et*k a(- )*as*ka( ber(*la* e;ekt*; )a( e;*s*e($ Asa sal*s*lat er"!aka( obat a(- bers*;at asa lea$ 9ar* !ersaaa( a()erso(>asselba e(ataka( ba@a obat a(- bers*;at asa lea aka( ba(ak terabor!s* !a)a ! l*(-k"(-a( a(- re()a e(er"s, se*(--a e(ebabka( ter:a)*(a -ra)*e( ka)ar$
VIII.
#esi"pulan
Proses absor!s* obat elal"* sal"ra( !e(er(aa( seara *( *tro )*!e(-ar"* ole ! )*a(a !a)a !erobaa( *(* e(--"(aka( sa!el obat asa sal*s*lat$ Asa sal*s*lat !a)a ko()*s* ! 1,2
be(t"k olek"l )ar*!a)a )ala be(t"k *o( se*(--a obat seak*( ")a terabsorbs* )a( e(eb"s ebra($ Ko(se(tras* asa sal*s*lat )* )ala lab"(leb* t*(--* )ar*!a)a )* )ala "s"s, kare(a absor!s* asa sal*s*lat )* lab"(leb* besar )ar*!a)a )* "s"s$ Asa sal*s*lat )* )ala "s"s
Da&tar Pusta'a
Bo"lto(, Toas B$ 1554$ Anestesiologi$ 'akarta ? Pe(erb*t B"k" Ke)oktera( E+/$ /a*r(s, 9$ 2003$ Intisari Kimia Farmasi Edisi 2$ 'akarta ? B"k" Ke)oktera( E+/$
M"tarasa(, R$, &a(e, M$, Ma--*e, &$ 2000$ Engineering Biotechnology terse)*a
o(l*(e
)*
tt!?CC@@@$-ate@aoal*t*o($or-C;*lesC*))e(C)el*C/.C.2;$t J)*akses ta(--al 25 Se!teber 2016=$ R*)@a(, E()*$ Et*ka
Pea(;aata(
e@a(
Perobaa(
)ala
Pe(el*t*a(
Keseata(. J Indon Med Assoc. 8ol"? 6., Noor? ., Maret 201., R"a Sak*t /*!to Ma(-"(k"s"o$ 'akarta$ Saka*, '$B$ 2005$ ractical harmacology !or "he harmacy "echnician. P*la)el!*a, U(*te) States ? L*!!*(ott &*ll*as &*lk*(s$ Sar-el
L,
&">Po(-
S,
" B$/$200$ Applied Biopharmaceutic
and
harmaco#inetics.t e)$ USA ? M$ +ra@ *ll$ /o!a(, I($ Sar-el, L$ &">Po(-, S$ )a( ", A$ B$ /$ 2012$ Bio!armaseti#a dan Farma#o#ineti#a "erapan$ Edisi Kelima$ S"rabaa ? A*rla(--a U(*ers*t Press$ &atso(, 9$+$, 200#. Analisis Farmasi$ 'akarta? E+/$
