UNIVERSIDADE FEDERAL DO CEARÁ CENTRO DE CIÊNCIAS DEPARTAMENTO DE BIOLOGIA BACHARELADO EM BIOTECNOLOGIA
METABOLISMO CELULAR – FERMENTAÇÃO E RESPIRAÇÃO AERÓBICA
JÉSSICA LOREN SARA PIRES VITÓRIA NOBRE
FORTALEZA 2014 Un!"#$%&%" F"%"#&' %( C"&#) C"n*#( %" C+n,&$ D"-&#*&."n*( %" B('(/&
Metabolismo Celular – Fermeta!"o e Res#ira!"o Aer$bi%a
Itro&u!"o
D"n"$" ."*&('$.( ,(.( ( ,(n3n*( %&$ &*!%&%"$ ."*&5',&$ %& ,6''& #"'&,(n&%&$ ,(. & *#&n$(#.&78( %" "n"#/&9 A (*($$:n*"$" " & #"$-#&78( $8( ($ -#(,"$$($ .&$ .-(#*&n*"$ %" *#&n$(#.&78( %" "n"#/& %($ $"#"$ !!($; .&$ & "#."n*&78( " & <.($$:n*"$" *&.6. $8( -#(,"$$($ ,"''&#"$ %" *#&n$(#.&78( %" "n"#/& .-(#*&n*"$ -&#& &'/n$ $"#"$ !!($9 A #"$-#&78( ,"''&# 6 . "n=."n(; (,(##%( %"!%( > &*!%&%" .*(,(n%#&'; <" ,(n$$*" n& "?*#&78( %& "n"#/& <:.,& &,.'&%& ". .('6,'&$ (#/@n,&$ %!"#$&$; ,(.( ,&#(%#&*($ " '-:%($; &*#&!6$ %& (?%&78( %"$$"$ ,(.-($*($ %" &'*( *"(# "n"#/6*,(; ,(. -#(%78( %" /)$ ,&#=n,( " )/&; &'6. %& '"#&78( %" "n"#/&; *'&%& -&#& <" (,(##&. &$ %!"#$&$ &*!%&%"$ ,"''&#"$9 A "#."n*&78( 6 . -#(,"$$( %" /"#&78( %" "n"#/& n( <&' (,(##" & (?%&78( n,(.-'"*& %" $$*@n,&$ (#/@n,&$; ". ,(n*#&-($78( > (?%&78( ,(.-'"*& <" (,(##" n& #"$-#&78( ,"'' N(#.&'."n*"; ( -#(,"$$( (,(##" &$$(,&%( & ,(n%7"$ &n&"#5&$ &$+n,& %" O 29 P(#6.; %"-"n%"n%( %( (#/&n$.( ( *-( ,"''&#; & "#."n*&78( -(%" (,(##"# n& -#"$"n7& %" O29 A #"$-#&78( ,"''&#; &( ,(n*#)#(; 6 . -#(,"$$( &"#5(; %" (,(##+n,& ($"#!&%& &-"n&$ n& -#"$"n7& %" O 29 O3"*!($"; ,(. ( -#(,"%."n*( -#)*,(; %".(n$*#&# "?-"#."n*&'."n*" ($ -#(,"$$($ %" "#."n*&78( " #"$-#&78( &"#5,&9
Materiais e m'to&os
O -#(,"%."n*( -#)*,( ( $%!%%( ". *#+$ -&#*"$ %".(n$*#&78( %& &*!%&%" %& "#."n*&78( ". '"!"%( Saccharomyces cerevisiae; *"$*" n%,&%(# %" -H " %".(n$*#&78( %($ -#(,"$$($ %" "#."n*&78( " #"$-#&78( &"#5,&9 D#&n*" & -#."#& -&#*" %& "?-"#."n*&78(; (#&. *'&%($ %($ *($ %" "n$&(; %"!%&."n*" "*<"*&%($ ,(.( LV '"!"%( !!( " LM '"!"%( .(#*(9 O *( "*<"*&%( ,(.( LV ( -#""n,%( ,(. .& $('78( %" '"!"%( !!(; ,(. &?:'( %" .& --"*& -')$*,&9 C(. & ."$.& $('78( %" '"!"%( !!(; -#""n,"$" . *( %" D#&.9 E. $"/%&; ( *( %" D#&. ( %"-($*&%(; ,(. $& &"#*#& !('*&%& -&#& &?(; %"n*#( %( *( %" "n$&( "*<"*&%( ,(.( LV9 L(/( &-5$; #"-"*$" ( -#(,"%."n*( ,(. '"!"%( .(#*( ( *( "*<"*&%( ,(.( LM ( -#""n,%( ,(. & $('78(; ,(. .& --"*& -')$*,& %"#"n*" %& *'&%& ,(. & $('78( %" '"!"%( !!(9 C(. & ."$.& $('78(; -#""n,"$" (*#( *( %" D#&.9 E. $"/%&; #"-"*$" ( -#(,"%."n*( ( *( %" D#&. ( 3(/&%( %"n*#( %( *( %" "n$&( "*<"*&%( ,(.( LM; ,(. &"#*#& !('*&%& -&#& &?(9 N& $"/n%& -&#*"; (#&. *'&%($ *#+$ *($ %" "n$&(; "*<"*&%($ ,(.( A ),%(; B )$,( " N n"*#(9 C(. .& --"*& "n,&?&%& ". .& -"#& %" $,78(; ( ,('(,&%( 1.' %" &' %" #(.(*.(' ". ,&%& . %($ *#+$ *($9 E. $"/%&; (#&. &,#"$,"n*&%&$ *#+$ /(*&$ %" .& $('78( ),%& n( -#."#( *(; *#+$ /(*&$ %" .& $('78( )$,& n( $"/n%( *( " *#+$ /(*&$ %" .& $('78( n"*#& n( *"#,"#( *(9 N& *"#,"#& -&#*"; (#&. *'&%($ $"*" *($ %" "n$&( /#&n%"$; "*<"*&%($ ,(.( C ,(n*#('"; LV '"!"%( !!(; LM '"!"%( .(#*(; FV "38( !!(; FM "38( .(#*(; FLV ('& !!& " FLM ('& .(#*&9 N( *( "*<"*&%( ,(.( C (#&. &,#"$,"n*&%($ 2.' %" $('78( %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9 N( *( "*<"*&%( ,(.( LV (#&. &,#"$,"n*&%($ 2.' %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; . -"%&7( %" &'/(%8( "."%( ". $('78( %" '"!"%( !!(; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9 N( *( "*<"*&%( ,(.( LM (#&. &,#"$,"n*&%($ 2.' %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; . -"%&7( %" &'/(%8( "."%( ". $('78( %" '"!"%( .(#*(; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9 N( *( "*<"*&%( ,(.( FV (#&. &,#"$,"n*&%($ 2.' %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; %" /#8($ %" "3"$ !!($; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9 N( *( "*<"*&%( ,(.( FM (#&. &,#"$,"n*&%($ 2.' %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; %" /#8($ %" "3"$ .(#*($; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9 N( *( "*<"*&%( ,(.( FLV (#&. &,#"$,"n*&%($ 2.' %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; .& ('& !!& -,&%&; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9 N( *( "*<"*&%( ,(.( FLM (#&. &,#"$,"n*&%($ 2.' %" &' %" #(.(*.('; . $-(#*" %" &'/(%8(; .& ('& .(#*& -,&%&; .& #('& %" &'/(%8( " .& *&.-& %" -&-"' &'.:n(9
Resulta&os e &is%uss"o
O "?-"#."n*( *"!" n:,( ,(. & ($"#!&78( %& &*!%&%" %& "#."n*&78( ". '"!"%(9 O$"#!($" <"; n( *( %" "n$&( ,(. ( '"!"%( !!(; ( *( %" D#&. "'"!($" &*6 & (#%& %( *( %" "n$&( ,(. & $('78( %" '"!"%(; %"!%( &( -#(,"$$( %" -#(%78( " '"#&78( %" CO 2 #"$'*&n*" %& "#."n*&78( #"&'&%& -"'( '"!"%( !!(9 J) n( *( %" "n$&( ,(. ( '"!"%( .(#*(; ( *( %" D#&. n8( &-#"$"n*( &'*"#&7"$ ". $& &'*#&; %"!%( &( &*( %" <" ( '"!"%( .(#*( n8( #"&'& "#."n*&78(9 N& $"/n%& -&#*" %( "?-"#."n*(; ( #"&'&%( . *"$*" %" n%,&%(# %" -H ,(. ( &' %" #(.(*.('9 O$"#!($"; n( *( %" "n$&( ,(. $('78( ),%&; ,('(#&78( &.&#"'&; n( *( %" "n$&( ,(. $('78( )$,&; ,('(#&78( &'; " n( *( %" "n$&( ,(. $('78( n"*#&; ,('(#&78( !"#%"9 O &' %" #(.(*.(' 6 . ),%( (#/@n,( #&,(9 E. ."( ),%(; &-#"$"n*& ,('(#&78( &.&#"'&; "n<&n*( <" ". ."( )$,( $& ,(# .%& -&#& &'9 A .%&n7& %" ,(# (,(##" ". ,"#*($ n*"#!&'($ %" -H; %"n(.n&%($ &?&$ ( n*"#!&'($ %" !#&/".9 &n%( ( !&'(# %( -H "$*) %"n*#( %& &?& %" !#&/".; (#.&$" .& ,(# n*"#."%)#& "$!"#%"&%&9 C(. ( *"$*" %" n%,&%(# %" -H; ( -($$:!"' &"# & &n)'$" %($ *($ %" "n$&( -#"-&#&%($ %#&n*" & *"#,"#& -&#*" %( "?-"#."n*(; ". <" ( .&*"#&' ('5/,(; ( &' %" #(.(*.(' " ($ $-(#*"$ %" &'/(%8( (#&. $('&%($ ,(. -&-"' &'.:n(9 O$"#!($" <" n($ *($ %" "n$&( ,(n*"n%( .&*"#&' .(#*( n8( (!" &'*"#&7"$ n( -H; %"!%( > &$+n,& %" &*!%&%" ."*&5',& n($ *($9 J) n($ *($ %" "n$&( ,(. .&*"#&' !!(; ( -($$:!"' ($"#!&# &'*"#&7"$ n($ !&'(#"$ %" -H ( *( %" "n$&( ,(. '"!"%( !!( &-#"$"n*( ,('(#&78( &.&#"'&; n%,&n%( . -H ),%(; ( *( %" "n$&( ,(. "38( !!( &-#"$"n*( ,('(#&78( !"#%"; n%,&n%( -H n"*#( "; -(# .; ( *( %" "n$&( ,(. ('& !!& &-#"$"n*( ,('(#&78( &'; n%,&n%( -H )$,(9 E$$&$ &'*"#&7"$ (#&. #"$'*&%( %& (#.&78( %" CO 2 %#&n*" &$ #"&7"$ ($"#!&%&$9 D& #"&78( %( C( 2 ,(. & )/&; (,(##" & (#.&78( %" ),%( ,&#=n,(; H2CO; <" *(#n( ($ ."($ #"&,(n&$ .&$ %#(/"n&%($9 O ),%( n*"#&/ ,(. ( &' %" #(.(*.('; -#(!(,&n%(; &$$.; &$ &'*"#&7"$ n( -H %" ,&%& *( ". &n)'$"9
Re(er)%ias biblio*r+(i%as
COSTA; I*&/&#& R"#(9 Maual &e Aulas Pr+ti%as &e Biolo*ia Celular,
Re-ista Ci)%ia A*ro.mi%a, D$-(n:!"' ". **-KKK9,,&#"!$*&9,9#$""#n%"?9--,,&#"!$*&9 A,"$$( ". 200420149
Res#ira!"o %elular aer$bi%a e (ermeta!"o, D$-(n:!"' ". **-"%,&,&(9/'((9,(.('(/&&$$n*($('(/& ,"''&##"$-#&,&(,"''&#&"#(,&""#."n*&,&(9*.'9 A,"$$( ". 240420149
Metabolismo mi%robiao, D$-(n:!"' ". **-'(/9,,&9$, #'&.&.'"$20100&'&."*&('$.( .,#(9-% 9 A,"$$( ". 240420149
Metabolismo eer*'ti%o, D$-(n:!"' ". **-KKK9#&$'"$,('&9,(.('(/&."*&('$.(,"'' *.9 A,"$$( ". 240420149
Res#ira!"o %elular, D$-(n:!"' ". **-KKK9.n%("%,&,&(9,(.('(/&#"$-#&,&(,"'' *.9 A,"$$( ". 240420149
Fermeta!"o, D$-(n:!"' ". **-.&?&/9'(/$-(*9,(.9#20120"#."n*&,&(9*.'9 A,"$$( ". 240420149
A/ul &e Bromotimol0 C12314Br 1O5S, D$-(n:!"' ". **-
Fi*ura 76 S('7"$ ),%&; )$,& " n"*#&; A' %" B#(.(*.(' " $('7"$ %" '"!"%( !!( " '"!"%( .(#*( SHA0C?N2VC'<RF'I(B!?
A/ul &e Bromotimol, D$-(n:!"' ". **-KKK9n("$,('&9,(.<.,&&'%"#(.(*.('9 A,"$$( ". 240420149
A#)&i%e A6 Materiais utili/a&os Fi*ura 16 P&-"' &'.:n(; ('&$ !!&$ " .(#*&$ " "*<"*&$ -&#& .&#,&78( %($ *($ %" "n$&(
Fi*ura 86 T($ %" "n$&(; --"*& P&$*"#; &$*"$ %" !%#(; --"*& P&$*"#; /#8($ %" "38( !!( " "38( .(#*(; )/& %"$*'&%&; &'/(%8(; -"n"#&; *($ %" D#&.; !&$'& %" -')$*,( " -"#&
A#)&i%e B6 Resulta&os
Fi*ura 96 D& "$<"#%& -&#& & %#"*&; %" ,.& -&#& &?( X -#(,"%."n*( 1 &-5$ ($"#!&78( %&$ #"&7"$ ,(. '"!"%( .(#*( " '"!"%( !!(; #"$-",*!&."n*"Y -#(,"%."n*( 2 &-5$ ($"#!&78( %&$ #"&7"$ ,(. $('78( )$,&; n"*#& " ),%&; #"$-",*!&."n*"Y -#(,"%."n*( &-5$ #"&7"$ ,(. $('78( ,(n*#('"; "38( .(#*(; "38( !!(; '"!"%( .(#*(; '"!"%( !!(; ('& .(#*& " ('& !!&; #"$-",*!&."n*"9
