Chem Written Report

Written Wr itten Output on Pre-Laboratory Assignment Of  Extraction Group 7 Rose Ann Pasayloon Kenneth Neal Bona Deanna Loie a!le

Introduction "#tra$tion is a %ery $ommon laboratory pro$e!ure use! &hen isolating or purifying a pro!u$t' "#tra$tion is the &ith!ra&ing of an a$ti%e or a &aste substan$e from a soli! or a li(ui! mi#ture &ith a li(ui! sol%ent' Organi$ $hemistry employs soli!li(ui!) li(ui!-li(ui!) an! a$i!-base e#tra$tions'

Pre-Laboratory Questions: *' What is the principle involved in extraction? he prin$iple in%ol%e! in e#tra$tion is mainly separating a parti$ular  $omponent of a mi#ture by !issol%ing the soluble $omponent of a soli! mi#ture into a parti$ular sol%ent) allo&ing t&o layers to form) an! !raining off the bottom layer that $ontains the insoluble $omponent using a separatory funnel' +' Draw the structures of caffeine theobro!ine theophylline and the flavonoid and tannin present in the tea" O H3C

O

CH3 N

N N

N

CH3

,affeine

heophylline

la%onoi! .,ate$hins/

heobromine

annin .la%an-0-ol/

# What are $annins? #

annins are a $lass of $ompoun!s in tea) espe$ially bla$1 tea) &hi$h ten! to ha%e a  bitter fla%our an! astringent properties' eas high in tannins $an be !es$ribe! as tanni$' hey are also responsible for the !ar1 $olours in some streams) as they are foun! in lea%es an! &oo!) an! are release! as organi$ matter brea1s !o&n' # What are %lavonoids? #

la%onoi!s are !ietary $ompoun!s naturally foun! in tea) &ine) $o$oa) fruits an! %egetables' hey $ontribute signifi$antly to taste an! $olor an! are $onsi!ere! to help maintain $ertain normal) healthy bo!y fun$tions' la%onoi!s are a group of plant metabolites thought to pro%i!e health benefits through $ell signaling path&ays an! antio#i!ant effe$ts' hese mole$ules ar e foun! in a %ariety of fruits an! %egetables'

&" What is the role of sodiu! carbonate in the extraction of caffeine fro! tea leaves? he e#tra$te! li(ui! mi#ture from the tea lea%es in$lu!e tannins an! the $affeine' 2n or!er for us to isolate the $affeine from other $ompoun!s) &e &ill !issol%e the 3o!ium ,arbonate to the &ater in &hi$h the tea bags &ill be boile!' 3o!ium ,arbonate a$ts as the base' 3in$e tannins are a$i!s) the so!ium $arbonate &ill rea$t &ith the tannins forming the so!ium salt that is highly soluble in &ater' 2f the tannins are !issol%e! in &ater) &e $an pre%ent it from e#tra$ting together &ith the $affeine &hen e#tra$ting the $affeine from the bre&e! tea' '" Which of the two layers that for!ed durin( extraction is dichloro!ethane? Explain your answer" he $olourless layer) &hi$h is also the upper layer that forme! !uring the e#tra$tion is the !i$hloromethane' Di$hloromethane is use! to isolate the $affeine an! &ater from the mi#ture &hi$h $onsists of the &ater &ith so!ium $arbonate an! the tea lea%es e#tra$t'