DTM&H;DMM
PARASITOLOGY-amoeba
NCAMD;
Introduction to Protozoology Protozoans : > sn!"e #e"" or $n#e""$"ar or!ansm > % !enera" sta!es; a' Tro()ozote or *e!etat*e or +ee,n! sta!e b' Cst# sta!e . non-mo non-mot"e t"e A' Mor()o"o!:
*ar n sze . /' #o" . P"asmo,$m
S)a(e 0 assmetr#a" . C' mesn" 0 b"atera" smmetr . G'"amb"a 0 #$bo,a" . /' #o"
Introduction to Protozoology Protozoans : > sn!"e #e"" or $n#e""$"ar or!ansm > % !enera" sta!es; a' Tro()ozote or *e!etat*e or +ee,n! sta!e b' Cst# sta!e . non-mo non-mot"e t"e A' Mor()o"o!:
*ar n sze . /' #o" . P"asmo,$m
S)a(e 0 assmetr#a" . C' mesn" 0 b"atera" smmetr . G'"amb"a 0 #$bo,a" . /' #o"
Tro()ozote T ro()ozote sta!e : >1 n$#"e$s . /' #o"; G' "amb"a; & D' +ra!"s no mo$t) . #tostome 2 /' #o"
>
/' Cto("asm: 1' 3#to("asm 0 o$ter "aer4 "ess !ran$"ar4 more )omo!eno$s %' 3n,o("asm 0 +oo, *a#$o"es . #)romato,a" bars5 !"#o!en 6$n#ton o+ #to("asm: 1' Res(raton %' Prote#ton 7' In!eston 8' Lo#omoton . Pse$,o(o,a4 9a!e""a4 #"a
N$#"e$s 0 #om(ose, o+ #)romatn matera"s . re(ro,$#ton T(e T (e o+ N$#"e$s 1' es#$"ar es#$"ar t(e :a sn!"e mass or an a!!re!ate o+ #)romatn arosome 0 m,,"e o+ t)e n$#"e$s or e,!e o+ t)e n$#"e$s4 s$rro$n,e, b #)romatn (art#"e (art#"es s4 arran!e, n a net
Ot)er str$#t$res: 1' Ctostome or #e"" mo$t) %' Ce"" an$s or #to()a!e 7' 3#retor *a#$o"e 8' Contra#t"e *a#$o"e ?' Ner*o$s sstem .#"ates & 9a!e""ates =neto("ast @' neto("ast
P)so"o! o+ Paraste & Metabo"sm
6ree-"*n! s(e#es retan aerob# metabo"# (at)
Parast# s(e#es are +a#$"tat*e or ob"!ate anaerobes
> ntestna" (rotozoa . 3' )sto"t#a4 s$r**es & (ro(a!ate n "o< O tenson' > b"oo, & tss$e 9a!e""ates reB$res )ematn or !ro Ma"ara" (arastes obtan metabo"tes +rom )emo!"obn +rom o", R/C o+ )ost & amno a#,s +rom ("asma o+ )ost'
L+e ##"e Re(ro,$#ton 0 sm("e bnar sson ' .Ase$a" re(ro,$#ton 6"a!e""ates : "on!t$,na" as C"ates
: trans*erse "nes
S$r**a" Me#)ansm o+ Protozoan; 1' Person to (erson trans+er : . (rotozoans <t) tro()# sta!e on" %' 3n#staton or #st +ormaton 7' 3#staton 8' Se$a" ()ase
Laborator Da!noss 1' Stoo" eamnaton: >Tro()ozotes : so+t4 "B$,4 or "oose #onssten#
Cst
: +orme, stoo"s
a' Sa"ne
AMO3/A
P)"$m Protozoa S$b()"$m Sar#o,na C"ass R)zo(o,a 6am" 3ntamoeb,ae ? Im(ortant Genera :1' 3n,amoeba %' 3ntamoeba 7' 3n,o"ma 8' Io,amoeba ?' Dentamoeba DEerentate, b t)e n$#"ear str$#t$res
3ntamoeba )sto"t#a Dsease : > Amebass > Amoeb# ,senter > Amoeb# #o"ts > Amoeb# "*er abs#ess > amoeb# bran abs#ess > C$taneo$s amebass Geo!ra()# Dstrb$ton: Pre*a"ent n tro(#s t)an n #oo"er #"mates Transmsson : #ontamnate, +oo, & ,rn=s <t) #st
3' )sto"t#a Trophozoite : Sze ran!e Mot"t 0
Cyst: F-@?m
(ro!ress*e4 n!er-"=e (se$,o(o,a
o+ n$#"e 0 one arosome 0 sma"" & #entra" Per()era" #)romatn 0 ne & e*en" ,strb$te, Cto("asm 0 ne" !ran$"ar Cto("asm# n#"$sons 0 n!este, R/C
Sze ran!e S)a(e ro$n,
F- %% m s()er#a" to
o+ n$#"e
128
arosome
sma"" & #entra"
Per()era" #)romatn 0 ne & e*en" ,strb$te, Cto("asm
0 ne" !ran$"ar
Cto("asm# n#"$sons 0 #)romato, bars5 ro$n,e, en,s4 ,E$se !"#o!en mass
3(,emo"o! . 3' )sto"t#a > 1J "ea,n! #a$se o+ (arast# ,eat)s > s$btro(#a" & tro(#a" areas >a"so est n #o",er #"mates . A"as=a4 R$ssa & Cana,a > areas o+ (oor santaton4 menta" )ea"t) +a#"t4 (rsons & ,a #are #enters > areas <)ere )$man )omose$a" #omm$ntes > ora"-+e#a" transmsson . $n(rote#te, se > m(ro(er treatment o+
Sm(tomato"o! : > Protean or *are, ,e(en,n! on ste & etent o+ tss$e ,ama!e IP' /o"o!#a" 0 1-? ,as C"n#a"
0 8 ,as to a ear
> GI sm(toms4 ,senter# stoo"s <t) b"oo, & m$#$s4 ab,omna" #ram(s4 na$sea4 *omtn! > L*er abs#ess4 $((er r!)t ab,omna" (an4 ameb# bran abs#ess0 $s$a"" +ata" ' D on" n ne#ro(s
Pat)o!eness & Pat)o"o! Co"onzaton n #o"on ,e(en,s on; 1' n$mber o+ a#t*e tro()ozotes %' )(ermot"t 7' s$tab"e enter# ba#tera 8' (at)o!en# n,e > sma"" ra#e "ess t)an 1m#ra4 non(at)o!en# > "ar!e ra#e more t)an 1 m#ra 4 (at)o!en#
A' Intestna" "esons : a' Prmar "esons 0 #e#$m4 a((en, & as#en,n! #o"on' m$#osa- m$s#$"ars 2 s$bm$#osa /ott"e-ne#= "eson #a$se, b "t# ne#ross b' Se#on,ar "esons 0 ot)er "e*e"s o+ t)e ntestnes & etrantestna" or!ans & tss$es'. amoeb# !ran$"oma /' 3tra-ntestna" "esons : > )emato!eno$s > ,re#t etenson > "m()at# ro$te
6"as= S)a(e, "#er
Da!noss : 1' Hstor & #"n#a" man+estatons %' -ra ""n! ,e+e#t <t) bar$m mea"; 7' L*er s#an 8' Pro#tos!mo,os#o(# eam ?' Stoo" eam T: 1' Metron,azo"e .6"a!" %' Tn,azo"e . 6as!n 7' Antbot#s . Tetra##"ne
Pre*enton & #ontro" > Ins(e#ton o+ +oo, ser*#e . )an,"ers4 ater treatment re!men > Pro(er a*o, $se o+ )$man +e#es as +ert"zer > !oo, (ersona" )!ene & santaton (ra#t#es > 6oo, (rote#ton +rom 9es & #o#=roa#)es > a*o,an#e o+ $n(rote#te, se$a" (ra#t#es
3ntamoeba #o" Trophozoite
Cyst
Sze ran!e
1% -?? m
F -7?m
Mot"t
Non (ro!ress*e5 b"$nt
N$mber o+ n$#"e
one
=arosome
Lar!e4 rre!' e##entr#
Per()era" #)romatn
Coarse4 $ne*en ,str
Cto("asm
#oarse
Cto("asm# n#"$sons
/a#tera 4 ot)er ,ebrs
S)a(e
1 -F
T)n #)romato, barss("ntere, en,s4 ,E$se !"#o!en mass Ro$n, to s()er#a"
Differentiation between E. histolytica and E. coli: E. histolytica
E. coli
R/C n #to("asm
(resent
absent
C)romatn bo,
C!ar s)a(e
S("nter "=e
C"ear4 n!er "=e
/"$nt
Mo*ement
Dre#tona" . a#t*e
S"$!!s)
N$#"e$s . #st
8
F
In+e#t*e sta!e
Cst
Cst
Lo#aton o+ =arosome
#entra"
(er()era"
Pat)o"o!#
Yes . 9as=-s)a(e $"#er
No
T(e o+ (se$,o(o,
3ntamoeba #o"
3n,o"ma nana Trophozoite
Cyst
Sze ran!e
?-1%
8-1%
Mot"t
S"$!!s)4 non (ro!ress*e4 b"$nt
o+ n$#"e
one
1-8
arosome
Lar!e4 rre!4 b"ot"=e
--
Per()era" C)romatn
absent
--
Cto("asm
Gran$"ar an, *a#$o"ate, --
Cto("asm# n#"$sons
/a#tera
C)romatn !ran$"es4 !"#o!en mass
3n,o"ma nana #st
Sma""est (rotozoan Tro()ozote: #"ean #to("asm
Cst: 8 n$#"e
C"n#a" s(e#men: stoo"
3ntamoeba (o"e#=
most #ommon ntestna" ameba o+ )$mans n Pa($a4 Ne< G$nea Tro()ozote
Centra" =arosome
No R/Cs ns,e #to("asm
Cst
Sn!"e n$#"e$s
In#"$son bo,
C)romato,a" bars .*arab"e
3' (o"e#= Trophozoites
Cyst
Sze
F to %?
1 to %
Mot"t.norma" stoo"
S"$!!s) non(ro!ress*e
.,arr)ea" stoo" Pro!ress*e $n,re#tona" N$mber o+ n$#"e
1
1
=arosome
Sma"" an, #entra"
Sma"" an, #entra"
Per()era" #)romatn
6ne e*en" ,strb$te,
6ne e*en" ,strb$te,
#to("asm
Gran$"ar an, *a#$"ate,
Gran$"ar
Cto("asm# n#"$sons
In!este, ba#tera
C)romato, bars4an!$"ar or (onte, en,s G"#o!en mass or
Io,amoeba b$ts#)" Trophozoite
Cyst
Sze ran!e
F 2 %% m
?- %%m
Mot"t
S"$!!s)4 (ro!ress*e
----
o+ n$#"e$s
1
1
arosome
Lar!e4 $s$a"" #enta" Lar!e4 e##entr# Re+ra#t*e a#)romat# !ran$"es .Q5- A#)romat# !ran$"es mabe (resent
Per()era" #)romatn
absent
absent
Cto("asm
Coarse" !ran$"ar & *a#$o"ate, /a#tera4 east #e""s ot)er ,ebrs
Iodamoeba butschlii
Prominent glycogen cyst
/as=et n$#"e$s
Tro()ozote s *er sm"ar to 3' nana
