CPS ID 3 Folleto Con Colores

from diagnosis, the seeds of better health

ISOLATION and ENUMERATION of all urinary tract pathogens Colonies are well isolated and easy to identify with real differentiating colours. Microbial enumeration with colourless background to optimise colony counting (for inoculation and interpretation, see back page).

DIRECT IDENTIFICATION CPS ID 3, NEW generation contains specific substrates of the enzymatic activities to be detected.

GREATER PRATICABILITY ●

E.coli : ß-glucuronidase (ß-GUR). No indole test required.

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Proteus: spontaneous colouration of colonies producing deaminase. No additional TDA test required.

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Enterococci: ß-glucosidase (ß-GLU).

 P.mirabilis Enterococci

 E.coli 

Pink to burgundy colonies ß-glucuronidase + ß-glucosidase -

Light brown to dark brown colonies

Turquoise colonies

ß-glucuronidase - / ß-glucosidase deaminase +

ß-glucuronidase ß-glucosidase +

 Proteus  E. coli 

Direct examination

Detection of Indole Indole +

Indole -

Cocci

 Proteus indole +  Morganella, Providencia

Bacilli

 Proteus mirabilis

Enterococci

Follow up identification

GREATER RELIABILITY AND IMPROVED PERFORMANCE * The NEW GENERATION of CPS ID 3 has increased : ●

Nutrient capacity , mainly for staphylococci and yeasts.

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Sensitivity of detection and specificity of colouration for the main organisms usually found in urine.

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Identification of the bacterial group KESC (Klebsiella, Enterobacter , Serratia and Citrobacter ) KESC => better colour intensity , due to increased ß-glucosidase activity.

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Orientation of identification towards Streptococcus agalactiae.

 Klebsiella pneumoniae ➡

➡ ➡

Green to brownish-green colonies : ß-GLU + Direct examination : bacilli Complete the identification

 Pseudomonas aeruginosa ➡ ➡ ➡ ➡

Pigmented colonies Direct examination : mobile bacilli Oxidase + Complete the identification

Staphylococcus aureus Immediate identification with : ➡ Usually yellow colonies ➡ Direct examination : cocci ➡ Catalase + ➡ Slidex Staph Plus

Candida albicans Streptococcus agalactiae

➡

Usually violet colonies ➡ Presumption of   Streptococcus agalactiae ➡ Complete the identification

➡

➡

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White colonies Direct examination : yeasts Complete the identification

IDENTIFICATION OF POLYMICROBIAL MIXTURES

Mixed culture showing :  E.coli and Enteroccoci Mixed culture showing :  E.coli , Proteus and Enteroccoci Immediate identification with : ● ● ●

Pink to burgundy : E.coli 

Light brown to dark brown : Proteus

Turquoise : Enterococci

Mixed culture showing :  K.pneumoniae and Enteroccoci Mixed culture showing :  E.coli and P. mirabilis

Microbial enumeration : ●

Inoculation with a 10 µl calibrated loop.

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Interpretation of bacteriuria : < 104 CFU/ml : probable contamination between 104 and 105 CFU/ml : equivocal result, repeat test on a new specimen > 105 CFU/ml : probable infection

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Compare the density of the colonies detected with that illustrated below (CFU = Colony forming Unit) :

104 CFU/ml 103 CFU/ml

105 CFU/ml

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CPS ID 3 : Ref. 43 541 : kit of 20 plates - Ref. 43 549 : kit of 100 plates ID Indole TDA : Ref. 56 541 : 200 tests ID color Catalase : Ref. 55 561 : 100 tests Oxidase Reagent : Ref. 55 635 : 50 ampules RAPIDEC STAPH : Ref. 03 300 : 10 strips x 3 tests Slidex® Staph Plus : Ref. 73 115 : kit of 50 tests - Ref. 73 116 : kit of 250 tests bioMérieux offers a wide choice of media, including a comprehensive range of ready-to-use chromogenic media. Consult your local bioMérieux representative for further information and availability. * For more details see technical sheet

bioMérieux sa 69280 Marcy l’Etoile France  Tel. : (33) 04 78 87 20 00 Fax : (33) 04 78 87 20 90  www.biomerieux.com