Titrable Acidity of Urine

TITRABLE ACIDITY OF URINE GENERAL COMMENTS The kidneys are responsibe !or "he e#$re"ion o! non%&oa"ie a$ids prod'$ed as a res'" o!  (e"aboi$ pro$esses) The 'rine has a physioo*i$a p+ o! ,)-%.)/ 0i"h a (ean aro'nd 1)The a$idi"y o! 'rine is (ainy d'e "o "he presen$e o! a$id phospha"es 2+34O,%56 "he (inor $on"rib'"ion is (ade by or*ani$ a$ids ike 'ra"es6 o#aa"es6 pyr'&i$6 a$"i$ and $i"ri$ a$ids) These a$ids are e#$re"ed in "he 'rine as sa"s o! sodi'(6 po"assi'(6 $a$i'( and a((oni'() Ti"rabe a$idi"y o! 'rine is e#pressed as 7"he a(o'n" o! akai re8'ired "o "i"ra"e or  ne'"rai9e "he a$id presen" in 'rine:) In o"her 0ords6 i" is "he &o'(e o! ;)5) CONDITIONS ?+IC+ INCREASE T+E ACIDITY OF URINE 2LO? URINE p+5@ •

DIET@ Ri$h in pro"eins6 (ea"6 (ea"6 (ik6 $heese6 ri$e 0hoe 0hea" prod'$"s6 p'(s6 $herries and o"her a$id !or(in* !oods)

•

FASTINGSTA FASTINGSTAR RA ATION@ TION@ eads "o in$reased !a" (e"abois( 0hi$h prod'$es a$e"oa$e"i$ a$id and %hydro#y b'"yri$ a$ids

•

EERCISE@ i*oro's i*oro's e#er$ise eads "o "he prod'$"ion prod'$"ion o! a$"i$ a$id

•

FEER@ eads "o in$reased in$reased ra"e o! (e"abois( $a'sin* es$ape o! s'pha"es and  phospha"es in 'rine)

•

ACIDOSIS@ ACIDOSIS@ e)*) in DA 2Diabe"i$ 2Diabe"i$ e"oa$idos e"oa$idosois56 ois56 d'e "o a$k o! ins'in6 ins'in6 !a" $a"abois( $a"abois( eads "o prod'$"ion prod'$"ion o! ke"oa$ids6 ke"oa$ids6 a$e"oa$e"i$ a$e"oa$e"i$ and %hydro#y %hydro#y b'"yri$ a$ids)

•

In"ake o! a$id prod'$in* sa"s !or "he "rea"(en" o! 'rinary "ra$" in!e$"ion)

CONDITIONS ?+IC+ DECREASE T+E ACIDITY OF URINE 2+IG+ URINE p+5@ •

DIET@ A &e*e"abe &e*e"a be die"

•

4OST4RANDIAL ALALINE TIDE@ TIDE@ Sa(pes o! 'rine $oe$"ed shor"y a!"er a (ea (ay be akaine 2hi*h p+ o! "he 'rine5) Gas"ri$ a$id se$re"ion is ee&a"ed a!"er a (ea and s'!!i$ien" + ions are se$re"ed in"o "he *as"ri$ '(en6 "hereby raisin* "he p+ o! sys"e(i$ bood and (akin* "he 'rine akaine)

•

ALALOSIS@ d'e "o any $a'se)

•

A!"er "he in*es"ion o! akai !or "he "rea"(en" o! *as"ri$ and d'odena '$ers)

•

Fro( ba$"eria in "he 'rine 2par"i$'ary 4ro"e's spe$ies5 0hi$h *enera"e a((onia on de$o(posi"ion o! 'rea6 a $ons"i"'en" o! 'rine)

•

Urine (ay be$o(e akaine 'pon s"andin* be$a'se o! "he oss o! CO3 and "he $on&ersion o! 'rea in"o a((onia by ba$"eria a$"ion)

PRACTICAL

To de"er(ine "he "i"rabe a$idi"y o! per day 'rine) METHOD:

Foins (e"hod APPARATUS:

B're""e6 pipe""e6 "i"ra"ion !ask6 'rine Har6 b're""e s"and REAGENTS: •

;)< N NaO+ so'"ion

•

4henoph"haein < as an indi$a"or 

PROCEDURE:

Coe$" 'rine in a 'rine Har and "hen "ake <; ( o! 'rine in "he "i"ra"ion !ask) Add !e0 drops 2<%35 o! indi$a"or i)e) phenoph"haein "o i") Mi# "he $on"en"s o! "he !ask 0e) Fi# "he b're""e in "he s"and and !i i" 0i"h ;)< N NaO+ so'"ion) No"e "he ini"ia readin*)  No0 "i"ra"e "he $on"en"s o! "he !ask a*ains" ;)< N NaO+ so'"ion "aken in "he b're""e) Add NaO+ drop by drop 'n"i "he $oo'r o! "he $on"en"s o! "i"ra"ion !ask be$o(e  persis"en"y i*h" pink) This is "he end poin") No"e "he !ina readin* on "he b're""e) 2A0ays "ake "he o0er (enis$'s5) Take a" eas" J readin*s in a si(iar !ashion and !ind o'" "he (ean &o'(e o! NaO+ 'sed) CALCULATIONS:

o'(e o! NaO+ 'sed in "i"ra"ion K 7: ( For <; ( 'rine &o'(e o! NaO+ 'sed K 7: ( For
3;;  /;; (day