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Descripción: MICROBIOLOGIA-MEDIOS DE AULIVO DE AEROBIOS MESOLFILO
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Apusan darah diamati dengan mikroskop binokuler pada pembesaran objektif 100x dengan penambahan oil imersi. Diff count dilakukan pada counting area dimana eritrosit menyebar merata. Bentuk-b…Deskripsi lengkap
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Descripción: Count Basie - Lil Darlin
Total Count Bacteria
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Apusan darah diamati dengan mikroskop binokuler pada pembesaran objektif 100x dengan penambahan oil imersi. Diff count dilakukan pada counting area dimana eritrosit menyebar merata. Bentuk-b…Full description
piano arr.
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Full score of Count on Me by Bruno Mars for String Quartet. Violin 1 part.Descripción completa
B. Rees -Ecker Method y progenitor of Brecker & Cronkite y ordinary L/M y Diluent: Rees -Ecker diluent same as Dameshek method y Only 1 RBC pipet is needed y Dilution = 1:200 Procedure: (only the differences from B& C) a. draw blood up to 0.5 mark, then diluent up to 101 mark b. place the hemacytometer in a Petri Dish w/ wet cotton ball (to prevent evaporation) & allow to stand for 10mins c. examine under HPO of L/M d. count platelets in 25 R squares on each counting chamber (a total of 50 R squares) Calculat ion:
C. Guy & Leakes Method y modifies Rees-Ecker Method y Diluent: same as Rees-Ecker but uses crystal violet as dye y Ordinary L/M y 1 RBC pipet y Diluent to 1 mark, Blood to 0.5 mark, Diluent again to 101 mark (moistening of pipet is no longer necessary ) y Dilution = 1:200 y Platelets are counted in 25 R squares only y Calculation & NV: same as Brecker & Cronkite y Correction factor: 2000 III. ELECTRONIC METHOD
1) Electrical Impedance - Coulter Thrombocounter - Celloscope 401 2) Light Scattering - Autocounter NV: same as Brecker & Cronkite Pseudothrombocytopenia may occur when giant platelets are present confirm the count w/ Brecker & Cronkite Method Q UANTITATIVE PLATELET DISORDERS
A. THROMBOCYTOSIS
1. Myeloproliferative syndrome y Polycythemia vera y Thrombocythemia - platelet count as as 1,000,000/µl 2. Post Splenectomy 3. After admin of epinephrine 4. After blood loss (including surgery) 5. Accompanying BM recovery y After cytotoxic chemotherapy y After treatment of Vit B12 B. THROMBOCYTOPENIA 1. due to platelet production y Aplastic anemia y Paroxysmal nocturnal hemoglobinuria y Leukemia (acute, chronic) y Metastatic lymphoma or carcinoma y Folate & Vit B12 deficiency y Cytotoxic & immunosuppressive chemotherapy
y Drug-induced (Quinine, Quinidine, Penicillin & Sulfa drugs) y Bacterial infections (Diphtheria, Typhoid fever) 2. Due to Platelet Sequestration
Massive splenomegaly Liver diseases Portal hypertension Lymphomas 3. Due to Immune Destruction of platelet y Autoimmune thrombocytopenia - presence of anti-platelet IgG - formerly ITP (idiopathic thrombocytopenic purpura) 4. After Massive Blood Transfus ion - dilution of circulating platelets w/ banked blood - takes 3-4 days of platelet count to return to normal y y y y