LAPORAN PRAKTIKUM FISIOLOGY TUMBUHAN PENGARUH HORMON TERHADAP PEMANJANGAN JARINGAN
Oleh: Layyinatul Zuhriya 12030204241
INTERNATIONAL BIOLOGY EDUATION 2012
UNIVERSITAS NEGERI SURABAYA FAKULT FAKULTAS MATEMATIKA DAN ILMU ILM U PENGETAHUAN PEN GETAHUAN ALAM JURUSAN BIOLOGI 2014
BAB I PENDAHULUAN
A. Lata Latarr Bea! Bea!a" a"# #
!ertu"#uhan $an %er&e"#an'an tu"#uhan ialah ha(il intera&(i antara )a&t*r luar $en'an )a&t*r $ala"+ Intera&(i ter(e#ut "en'ha(il&an tu"#uhan yan' #er#e$a (atu $en'an yan' lainnya, #ai& $ala" hal u&uran #atan', -eni( #atan', -eni( %er#un'aan $an (e#a'ainya+ .a&t*r internal internal "eli%uti (i)at 'eneti& 'eneti& yan' ter$a%at ter$a%at $i $ala" 'en $an h*r"*n h*r"*n yan' "eran'(an' "eran'(an' %ertu"#uhan+ /e$an'&an )a&t*r e&(ternal a$alah )a&t*r lin'&un'an+ *r"*n ialah at %ertu"#uhan yan' "utla& $i"ili&i *leh tu"#uhan $ala" "ela&u&an a&ti)ita( a&ti)ita( &ehi$u%annya+ &ehi$u%annya+ *r"*n *r"*n tu"#uhan tu"#uhan ialah (uatu (enyaa *r'ani& yan' $i#uat %a$a (uatu #a'ian #a' ian tu"#uhan $an &e"u$ian $ian'&ut $ian'& ut &e & e #a'ian lain, %a$a &*n(entra(i ren$ah "enye#a#&an $a"%a& )i(i*l*'i(+ !eran h*r"*n "eran'(an' %ertu"#uhan, %e"#elahan (el, %e"an-an'an (el $an a$a yan' "en'ha"#at %ertu"#uhan+ *r"*n %a$a tu"#uhan (an'at #era'a" $an "e"%en'aruhi %ena"%a&an tu#uh tu"#uhan (e#a'ai ha(il $ari a&tiita(nya+ Au&(in a$alah (alah (atu h*r"*n tu"#uh yan' ti$a& terle%a( $ari %r*(e( %ertu"#uhan $an %er&e"#an'an 'r*th an$ $eel*%"ent5 (uatu tana"an+ Y*6i, Y*6i, 20075+ 8enurut Lar(en 19445 $an A#i$in 19725 In$*leaetal$ehy$e $ii$enti)i&a(i&an (e#a'ai #ahan au&(in yan' a&ti) $ala" tana"an+ /elan-utnya Lar(en 19;15< Bentley $an *u(tley 19;25 "en'e"u&a&an #aha at &i"ia ter(e#ut a&ti) $ala" "en(ti"ula(i %ertu"#uhan &e"u$i &e"u$ian an #eru#a #eru#ah h "en"en-a$ a$ii IAA+ IAA+ !eru !eru#a #aha han n ter( ter(e# e#ut ut "enu "enuru rutt G*r$ G*r$*n *n 19; 19;=5 =5 a$al a$alah ah %eru#ahan $ari Tri%th*%an "en-a$i IAA Thi"ann, 193;5+ Ber$ Ber$a( a(ar ar&a &an n urai uraian an $iat $iata( a(,, "a&a "a&a a&an a&an $ila $ila&u &u&a &an n %er %er*#a *#aan an yan' yan' "e"# "e"#an an$i $in' n'&an &an #er#a'ai "aa" -eni( h*r"*n AIA, 2+4 D $an NAA 1 %%"5 $an air (ulin' terha$a% %e"an-an'an (uatu -arin'an tu"#uhan+ B. R$%$ R$%$&a" &a" Ma&aa Ma&aa' '
Ber$a(ar&an latar #ela&an' ter(e#ut ru"u(an "a(alah %a$a %ra&ti&u" &ali ini a$alah : Ba'ai"ana %en'aruh #er#a'ai h*r"*n AIA, 2+4 D $an NAA 1 %%"5 $an air (ulin' $ala" %e"an-an'an -arin'an a&ar $an #atan' %a$a & ea"#ah -a'un' u"ur ; hari > (. T$)$a"
A$a%un tu-uan $ari %ra&ti&u" ini yaitu: 15 8e"#an$in'&an %en'aruh #er#a'ai h*r"*n tu"#uh terha$a% %e"an-an'an -arin'an a&ar $an &*le*%til+ 25 8en'etahui %en'aruh au&(in terha$a% %e"an-an'an -arin'an+ 35 8en'etahui %en'aruh h*r"*n AIA, 2+4 D $an NAA 1 %%" terha$a% %e"an-an'an a&ar $an &*le*%til -a'un'+
BAB II TINJAUAN PUSTAKA
Tu"#uh "eru%a&an %r*(e( %eru#ahan (eara &uantitati) yan' (i)atnya irreer(i#le $an #erlan'(un' (ela"a %er&e"#an'an (uatu *r'ani("e ter-a$i %ara"eter %eru#ahan $a%at $iu&ur $ala" (uatu -u"lah, u&uran, *lu"e $an #erat+ /alah (atu )at*r internal yan' "e"%en'aruhi %ertu"#uhan $an %er&e"#an'an tana"an a$alah h*r"*ne tu"#uhan+ Rahayu $an Lu&a(, 20115+ ?arin'an a$alah (e&u"%ulan (el yan' "e"ili&i #entu& $an )un'(i yan' (a"a+ A$a $ua -arin'an tu"#uhan yan' &ita &enal yaitu -arin'an "eri(te" $an -arin'an $ea(a+ ?arin'an "eri(ta" a$alah -arin'an yan' teru(@"eneru( "e"#elah+ ?arin'an "eri(te" $a%at $i#a'i 2 "aa" yaitu ?arin'an "eri(te" %ri"er $an -arin'an "eri(te" (e&un$er La&itan B, 20045+ ?arin'an "eri(te" a$alah -arin'an yan' (el@(elnya (elalu "e"#elah+ ?arin'an "eri(te" $a%at $i#a'i "en-a$i $ua "aa", yaitu "eri(te" %ri"er $an "eri(te" (e&un$er+ 8eri(te" %ri"er ter$a%at %a$a titi& tu"#uh $an "enye#a#&an %er%an-an'an a&ar $an #atan', (e$an'&an "eri(te" (e&un$er ter$a%at %a$a &a"#iu" $an "enye#a#&an tu"#uhan "en-a$i #e(ar /u'ih(ant*(a, 20095+ ?arin'an $ea(a a$alah -arin'an yan' ti$a& "eri(te"ati(+ ?arin'an $ea(a $a%at $i#a'i "en-a$i li"a "aa", yaitu: -arin'an e%i$er"i(, -arin'an %aren&i", -arin'an %eny*&*n', -arin'an %en'an'&ut, $an -arin'an 'a#u( La&itan B, 20045+ *r"*n "eru%a&an (uatu (enyaa *r'ani& yan' a%a#ila $ala" -u"lah &eil $a%at "eran'(an' %ertu"#uhan
(e$an'&an
#ila $ala"
-u"lah
#e(ar $a%at "en'ha"#at
%ertu"#uhan+ Dala" %en'ertian lain, $i$a%at&an #aha h*r"*n "eru%a&an (enyaa *r'ani& *r'ani& yan' $i(inte(i( $i (alah (atu #a'ian tu"#uhan $an $i%in$ah&an &e #a'ian lain $an %a$a &*n(entra(i yan' (an'at ren$ah "a"%u "eni"#ul&an (uatu re(%*n )i(i*l*'i(+ *r"*n tu"#uhan $iha(il&an $ala" &*n(entra(i yan' (an'at &eil, teta%i h*r"*n $ala" -u"lah yan' (an'at (e$i&it (a-a #i(a #er$a"%a& (an'at #e(ar terha$a% %ertu"#uhan $an %er&e"#an'an *r'an tu"#uhan+ /uatu h*r"*n #i(a #e&er-a $en'an ara "en'u#ah e&(%re(i 'en, "e"%en'aruhi a&tiita( eni" yan' a$a, atau $en'an ara "en'u#ah iri $an (i)at@(i)at "e"#ran+ /alah (atu $ari &er-a ini $a%at "en'arah&an &e"#ali "eta#*li("e $an %er&e"#an'an $ari (uatu (el yan' "ere(%*n (e-u"lah &eil "*le&ul h*r"*n+ Be#era%a
h*r"*n yan' #er%eran %entin' $ala" %ertu"#uhan $an %er&e"#an'an a$alah (e#a'ai #eri&ut: A. A$!&*"
Au&(in a$alah (alah (atu h*r"*n tu"#uh yan' ti$a& terle%a( $ari %r*(e( %ertu"#uhan $an %er&e"#an'an 'r*th an$ $eel*%"ent5 (uatu tana"an+
ata Au&(in #era(al $ari
#aha(a Yunani au6ein yan' #erarti "enin'&at&an+ /e#utan ini $i'una&an *leh .rit( ent 19=25 untu& (enyaa yan' #elu" $a%at $iiri&an teta%i $i$u'a (e#a'ai %enye#a# ter-a$inya %e"#en'&*&an &*le*%til &earah ahaya
Y*6, 20075+
!eran )i(i*l*'i( au&(in a$alah "en$*r*n' %er%an-an'an (el, %e"#elahan (el, $i)eren(ia(i -arin'an 6ile" $an )l*e", %e"#entu&&an a&ar, %e"#un'aan %a$a Bromeliaceae, %e"#entu&an #uah %arten*&ar%i, %e"#entu&&an #un'a #etina %a$a %a$a tana"an $i*i*u(, $*"inan a%ial, re(%*n(e tr*%i("e (erta "en'ha"#at %en''uran $aun, #un'a $an #uah+ !eranan Au&(in $ala" a&ti)ita( &ultur -arin'an au&(in (an'at $i&enal (e#a'ai h*r"*n yan' "a"%u #er%eran "en'in$u&(i ter-a$inya &alu(, "en'ha"#at &er-a (it*&inin "e"#entu& &l*r*)il $ala" &alu(, "en$*r*n' %r*(e( "*r)*'ene(i( &alu(, "e"#entu& a&ar atau tuna(, "en$*r*n' %r*(e( e"#ri*'ene(i(, $an au&(in -u'a $a%at "e"%en'aruhi &e(ta#ilan 'eneti& (el tana"an /u'ih(ant*(a, 20095+ B. G*+ere*"
Gi#erelin %erta"a &ali $ite"u&an *leh (e*ran' il"uan -e%an' yan' #erna"a E+ ur*(aa (aat "e"%ela-ari %enya&it %a$a tana"an %a$i a&i#at -a"ur Gibberella fujikuroi+ Gi#erelin $i&enal $en'an i(tilah GA yan' "e"%unyai 70 "aa", $i"ana yan' %alin' e)e&ti) $an (ta#il (erta yan' %alin' #anya& $i'una&an a$alah GA1, GA3 $an GA4, (e$an'&an GA29 "eru%a&an GA yan' ti$a& a&ti)+ GA3 "eru%a&an Gi#erelin yan' $i%r*$u&(i $an $ian'&ut (eara #e#a(+ !en'an'&utannya "en'i&uti -alur (inte(i( A(a" 8eal*nat $en'an %re&u(*rnya a$alah I!!+ GA3 "eru%a&an Ge#erelin a&ti) yan' $a%at "e"%en'aruhi %la(ti(ita( $in$in' (el $en'an 'ennya #eru%a Le $an le, a%a#la GA3 "enin'&at, "a&a &l*r*%la( a&an ter#entu& $en'an #a'u(+ E)e& 'i#erelin untu& "en$*r*n' %er%an-an'an #atan' $an terli#at $ala" %r*(e( re'ula(i %er&e"#an'an
tu"#uhan+ !a$a #e#era%a tana"an %e"#erian GA #i(a
"e"au %e"#un'aan $an "e"atah&an $*r"an(i tuna(@tuna( (erta #i-i i&i%e$iaC'i#erelin5 (. S*t,!*"*"
/it*&inin $ite"u&an *leh .+ /&**' yan' #era(al $ari DNA hean = )ur)uril 1@ a"in*%urin5+ /it*&inin "e"%unyai iri &ha( yan' $itan$ai $en'an a$anya (enyaa %urin+ /it*&inin $i%r*$u&(i $i a&ar tana"an $an $ian'&ut (eara a&r*%etal atau n*n %*lar+ /it*&*nin "eru%a&an h*r"*n yan' "en$*r*n' %e"#elahan (it*&ine(i(5+ Be#era%a "aa" (it*&inin "eru%a&an (it*&inin ala"i "i(al : &inetin, eatin5 $an #e#era%a lainnya "eru%a&an (it*&inin (inteti&+ /it*&inin ala"i $iha(il&an %a$a -arin'an yan' tu"#uh a&ti) teruta"a %a$a a&ar, e"#ri* $an #uah+ /it*&inin yan' $i%r*$u&(i $i a&ar (elan-utnya $ian'&ut *leh 6ile" "enu-u (el@(el tar'et %a$a #atan'+ .un'(i uta"a (it*&inin a$alah "eran'(an' %e"#elahan (el a&i#at %en'aruh %eran'(an'an (it*&inin terha$a% %e"#entu&an RNA $an eni" yan' (erin'&ali ter'an''u+ /it*&inin -u'a $a%at "e"%en'aruhi &ee%atan %ertu"#uhan (erta "eran'(an' tu"#uhan untu& "e"#entu& crown gall i&i%e$iaC(it*&inin5 D. A&a% A+&*&at -ABA
A(a" a#(i(at $ite"u&an *leh (e*ran' #er&e#an'(aan In''ri( yan' #erna"a !+.+ arein' $an .+T+ A$$i*t #er&e#an'(aan A"eri&a5, -alur (inte(i( ABA "en'i&uti A(a" "eal*nat $en'an %re&ur(*r uta"a #eru%a I!!+ .un'(i uta"a ABA $ala" tu"#uhan a$alah "enye#a#&an "enutu%nya (t*"ata -i&a ter-a$i (tre((+ euntun'an h*r"*n ABA, antara lain: 8e"#eri&an &etahanan %a$a #i-i tana"an %antai+ 8e"#uat $in$in' #i-i "en-a$i &uatC&e#al terha$a% Nal, (ehin''a ti$a& ter-a$i %la("*li(i(+ 8e"%ere%at %e"atan'an e"#ri*nal+ E. Et*e" -(2H4
Etilen $i%r*$u&(i %a$a $aun $ala" #er#entu& 'a(+ Etilen $a%at "eran'(an' %r*(e( %enuaan $an %e"atan'an %a$a #uah+ Etilen "e"%unyai -alur (inte(i( "eti*nin $en'an %re&ur(*r #eru%a a(a" a"in* "eti*nin+ !e"#entu&an etilen &arena a$anya ran'(an'an #eru%a lu&a yan' $a%at "en'ha"#at %er%an-an'an #atan', teta%i etilen "eran'(an' %ern''e"u&an #atan'+ /e"a&in "enin'&at (uhu, "a&a etilen a&an nai& yan' $a%at "en'a&i#at&an #anya& $aun yan' 'u'ur i&i%e$iaCetilen5
BAB III
METODE PENELITIAN
A. Je"*& Pe"e*t*a"
?eni( %enelitian yan' &a"i 'una&an a$alah e&(%eri"en &arena "en''una&an #e#era%a aria#el yaitu aria#el &*ntr*l, aria#el "ani%ula(i $an aria#el re(%*n+
B. Var*a+e Pe"e*t*a"
Faria#el yan' $i'una&an $ala" %enelitian ini, antara lain: 1+ Faria#el &*ntr*l: ?eni( &ea"#ah+ U"ur &ea"#ah+ U&uran %an-an' -arin'an yan' $iren$a" #ai& &*le*%til "au%un a&ar+ F*lu"e larutan AIA, larutan 2,4 D, larutan NAA, $an aua$e(+ ?u"lah %*t*n'an -arin'an &*le*%til $an a&ar yan' $iren$a"+ a&tu %eren$a"an+ 8e$ia %enyi"%anan+ 2+ Faria#el "ani%ula(i: ?eni( larutan $an -eni( -arin'an yan' $iren$a"+ 3+ Faria#el re(%*n(: !erta"#ahan %an-an' -arin'an yan' $iren$a" $n rata@rata %erta"#ahan %an-an'+ (. Aat /a" Ba'a"
1+ Alat: aan %etri /ilet ta-a" !en''ari( 2+ Bahan: ea"#ah -a'un' u"ur ; hari+ Di#uat %*t*n'an &*le*%til $an a&ar %ri"er $en'an
%an-an' ; "" $iu&ur %a$a -ara& 2 "" $ari &*tile$*n+ Larutan AIA Larutan 2,4 D Larutan NAA 1 %%"
D. La"#!a' Ker)a 1. 2.
8enyia%&an #ahan $an alat yan' $i%erlu&an+ 8enye$ia&an 30 #i-i #er&ulit &era( tia% &el*"%*& (atu "aa" #i-i #er&ulit &era(5 $an $ina'i "en-a$i 3 &el*"%*&:
8eren$a" 10 #i-i $ala" a(a" (ul)at %e&at (ela"a ; "enit, &e"u$ian $iui $en'an air+ Untu& 10 #i-i yan' lain $ihilin'&an #a'ian yan' ti$a& a$a le"#a'anya $en'an "en''una&an &erta( a"%la( $an &e"u$ian $iui $en'an air+ 8en'a"#il 10 #i-i yan' lain &e"u$ian ui $en'an air+ 3+ 8enana" &eti'a &el*"%*& #i-i ter(e#ut %a$a %*t yan' #er"e$ia tana" tanah $an %a(ir $en'an %er#an$in'an 1 : 1+ 8en'&*n$i(i&an %enana"an #i-i $ala" &ea$aan (a"a untu& &eti'a %*t+ 4+ 8en'a"ati %er&ea"#ahan untu& &eti'a %*t ter(e#ut (etia% hari (ela"a 14 hari+ 8enyira" tana"an (aat &*n$i(i (u$ah &erin'+ ;+ 8e"#uat ta#le %en'a"atan &ee%atan %er&ea"#ahan $ari ha(il %en'a"atan (au$ara+
E. A$r Pra!t*!
%$8enyia%&an #ahan $an alat yan' $i%erlu&an
8enye$ia&an %*t*n'an &*le*%til $an a&ar %inu( untu& tia%@tia% %erla&uan (e#anya& ; %*t*n'an
a$ah $en'an larutan AIA 1 %%" 10"l5
a$ah $en'an larutan 2,4 D 10"l5
a$ah $en'an larutan NAA 10 "l5
a$ah $en'an air (ulin' 10 "l5
Ditutu% $an $i#ia(&an (a"%ai 47 -a"
!en'u&uran &e"#ali
8e"#uat ta#el ha(il
BAB IV HASIL DAN PEMBAHASAN
A. Ha&* 1. Ta+e Ta+e 1. !en'aruh Ber#a'ai *r"*n Tu"#uh Terha$a% !e"an-an'an ?arin'an A&ar $an
*le*%til+
Je"*&
K,e,t* -%%
a!ar -%%
era!$a
Pa")a"
Pa")a"
Pert$%+$'
"
# aa
# a!'*r
a" a")a"#
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
H 7 10 7 = 20 11 H 7 9 13 7 10 H H H = = 7 H
7 3 ; 2 2 1; = 2 3 4 2 3 ; 4 1 2 1 1 3 2
NAA
2,4 D
AIA
Air /ulin'
2. Gra3*!
Ratarata ert$%+$'a " a")a"#
4
=
2,7
1,7
Pa")a"
Pa")a"
Pert$%+$'
# aa
# a!'*r
a" a")a"#
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
= = ; = = = H 7 H 9 H H ; H = = ; = = ;
1 1 0 1 1 1 2 3 2 4 2 2 0 2 1 1 0 1 1 0
Ratarata ert$%+$'a " a")a"#
1,4
2,4
0,7
0,=
7 6 5 4 KOLEOPTIL AKAR
3 2 1 0 NAA
2,4 D
AIA
Air Suling
Ga%+ar 1. !en'aruh Ber#a'ai !erla&uan Terha$a% !e"an-an'an ?arin'an A&ar $an
*le*%til+
B. A"a*&a Data (. PEMBAHASAN
Fer(i 1 !a$a -arin'an a&ar &an$un'an au&(in le#ih ren$ah $i#an$in'&an %a$a -arin'an &*le*%til+ al ini &arena (eara ala"i au&(in $i%r*$u&(i %a$a -arin'an "eri(te"ati& u-un' &*le*%til
yan' &e"u$ian $i$i(tri#u(i&an &e (eluruh tu#uh tu"#uhan untu& a&ti)ita(nya+ !a$a a&ar, a&ti)ita( %e"an-an'an ti$a& terlalu e&(tri" $i#an$in'&an $en'an a&ti)ita( %e"an-an'an %a$a -arin'an &*le*%til+ !e"#erian au&(in -eni( AIA "e"#antu a&ti)ita( %e"an-an'an -arin'an a&ar+ /e$an'&an %a$a %ena"#ahan 2,4 D $an NAA -u(tru "en'ha"#at a&ti)ita( %e"an-an'an -arin'an a&ar+ !a$a %erla&uan %e"#erian $en'an aua$e( a&ti)ita( %e"an-an'an (el teta% #erlan'(un'+ al ini $i(e#a#&an &arena a$anya au&(in yan' (eara ala"i telah $i$i(tri#u(i&an &e -arin'an a&ar untu& %e"an-an'an -arin'an+ Rata@rata %e"an-an'an -arin'an yan' $ita"#ah&an AIA le#ih tin''i $i#an$in' $en'an %e"#erian air (ulin' (e#a'ai &el*"%*& &*ntr*l+ !a$a %ena"#ahan 2,4 D $an NAA -u(tru "en'ha"#at %e"an-an'an, &arena nilai rata@rata %e"an-an'an -arin'an ter(e#ut le#ih ren$ah $i#an$in' &el*"%*& &*ntr*l yan' hanya $i#eri $en'an air (ulin'+ !a$a &*le*%til %ena"#ahan h*r"*n au&(in AIA5 a&an "e"au %e"an-an'an -arin'an+ /eara &i"ia, IAA (a"a $en'an a(a" a"in* tri%t*)an+ Be#era%a (enyaa yan' $i(inte(i( $a%at "eni"#ul&an re(%*n )i(i*l*'i (e%erti AIA, $ian''a% (e#a'ai au&(in yan' ter"a(u& &e$ala" &el*"%*& ini a$alah a(a" na)talena(etat NAA5, a(a" 2,4@$i&l*r*)en*&(i a(etat 2,4@D5 yan' )un'(inya -i&a $ileta&&an $ala" -arin'an "eri(te"ati& a$alah (e%erti ara &er-a au&(in+ /eara ala"i, au&(in $i%r*$u&(i *leh -arin'an "eri(te"ati& yan' a$a %a$a %uu&+ e#utuhan au&(in tana"an haru( #era$a %a$a &i(aran tertentu yan' *%ti"u" yan' $i#utuh&an *leh tu"#uhan+ !ena"#ahan au&(in $ala" -u"lah yan' #e(ar -u(tru "en'a&i#at&an "eta#*li("e (el@(el $ala" -arin'an "en-a$i &aau+ !a$a -arin'an &*le*%til yan' $i#eri AIA, -arin'an #erta"#ah %an-an' teta%i ti$a& (e%an-an' yan' $i#eri %erla&uan $en'an 2,4 D $an $en'an NAA+ !a$a -arin'an &*le*%til yan' $i#eri %erla&uan $en'an air (ulin' "enun-u&&an a$anya %erta"#ahan %an-an'+ al ini $i-a$i&an &el*"%*& &*ntr*l $i"ana *#-e& ti$a& $i#eri %erla&uan (e#a'ai"ana yan' lain+ ?arin'an &*le*%til a&an teta% "ela&u&an a&ti)ita( %e"an-an'an (el "e(&i%un ti$a& $i#eri au&(in+ al ini &arena %a$a &*le*%til (u$ah "e"%r*$u&(i au&(in (eara ala"i+ Oleh &arena itu a&ti)ita( %e"an-an'an (el a&an teta% #erlan'(un'+ /e$an'&an %ena"#ahan AIA -u(tru "en'a&i#at&an terha"#atnya a&ti)ita( %e"an-an'an (eara n*r"al yan' $i&arena&an terlalu #anya&nya AIA $ala" tu#uh -arin'an+ /e$an'&an %ena"#ahan 2,4 D $an NAA "e"#antu "enin'&at&an a&ti)ita( %e"an-an'an (el@ (el %a$a -arin'an &*le*%til+
Ber#a'ai -eni( h*r"*n #er%en'aruh terha$a% %e"an-an'an (el+ ?eni( h*r"*n tertentu (an'at (%e(i)i& terha$a% (uatu -arin'an tu"#uhan+ !e"an-an'an -arin'an %a$a a&ar a&an $i%en'aruhi *leh h*r"*n yan' #er#e$a $en'an h*r"*n yan' #er%en'aruh %a$a %e"an-an'an &*le*%til+ Ber$a(ar&an ha(il %en'a"atan (erta anali(i( yan' telah &a"i la&u&an, $i$a%at&an #aha %a$a -arin'an &*le*%til, h*r"*n 2,4 D "e"%en'aruhi a&ti)ita( %e"an-an'an -arin'an yan' (an'at e%at, (e$an'&an %a$a -arin'an a&ar -eni( h*r"*n AIA yan' yan' %alin' e%at "e"%en'aruhi %e"an-an'an -arin'annya+ !en'aruh h*r"*n ti$a& (a"a %a$a -arin'an yan' #er#e$a+ Fer(i 2 Dari $ata $an anali(i( $iata( "a&a $a%at $i&etahui #aha ter-a$i %e"an-an'an %a$a %*t*n'an -arin'an yan' $iren$a" $ala" larutan h*r"*n IAA, 2,4 D, NAA $an air (ulin' (e#a'ai aria#el &*ntr*l+ al ini $i&arena&an h*r"*nau&(in $a%at "e"au %e"#entan'an a&ar $an #atan' &arena au&(in "a"%u "en'en$ur&an $in$in' (el e%i$er"i( (ehin''a $in$in' e%i$er"i( yan' (u$ah &en$ur "en-a$i "en'e"#an' &e"u$ian (el e%i$er"i( ini "e"#entan' $en'an e%at $an %e"#entan'an ini "enye#a#&an (el (u# e%i$er"i( yan' "ene"%el %a$anya -u'a i&ut "en'e"#an'+ Ra$i&ula yan' $iren$a" $ala" NAA $an 2,4 D "enun-u&&an %e"an-an'an -arin'an le#ih (e$i&it $ari%a$a IAACAIA, &arena NAA $an 2,4 D "eru%a&an (enyaa (inte(i( au&(in yan' "enun-u&&an (tru&tur (e$i&it #er#e$a $en'an au&(in ala"i+ NAA $an 2,4 D ti$a& $iru(a& *leh IAACAIA *&(i$a(e &arena ti$a& a$a $ala" ra$i&ula+
/e$an'&an !a$a
%e"an-an'an -arin'an yan' %alin' #e(ar (aat $iren$a" $ala" AIA+ al ini $i(e#a#&an &arena AIA "eru%a&an au&(in ala"i+ Au&(in #anya& $i%r*$u&(i tu"#uhan $i &*le*%til+ !a$a ra$i&ula ti$a& ter$a%at AIA *&(i$a(e+ /aat ra$i&ula $iren$a" $ala" AIA ti$a& a$a AIA *&(i$a(e yan' $a%at "eru(a& AIA+ /ehin''a AIA a&an "eran'(an' %e"an-an'an ra$i&ula &ea"#ah -a'un'+ %a$a %eren$a"an ra$i&ula $ena'n aua$e(
-u'a "enun-u&&an %erta"#ahan rata@rata
-arin'an+ Teta%i %erta"#ahan %an-an'nya $i(e#a#a&an ter-a$inya *("*(i(+ !r*(e( *("*(i( ter(e#ut ter-a$inya &arena !O $an !O air (ulin' le#ih tin''i $i#an$in' !A $an !O -arin'an (ehin''a air #er%in$ah &e$ala" -arin'an+ *le*%til yan' $iren$a" $ala" AIA "enun-u&&an %e"an-an'an -arin'an le#ih (e$i&it $ari%a$a NAA, &arena AIA "eru%a&an h*r"*n au&(in ala"i yan' "e"%unyai (tru&tur (a"a $en'an AIA *&(i$a(e yan' ter$a%at %a$a &*le*%til+ /e$an'&an %erta"#ahan %an-an' -arin'an
yan' %alin' #e(ar (aat $iren$a" $ala" NAA+ al ini $i(e#a#&an &arena NAA "eru%a&an (enyaa (inte(i( yan' (tru&turnya "iri% au&(in+ Au&(in (en$iri #anya& $i%r*$u&(i tu"#uhan $i &*le*%til+ !a$a &*le*%til ter$a%at AIA *&(i$a(e $an eni"@eni" lain+ ?a$i (aat $iren$a" $ala" NAA, AIA *&(i$a(e ini ti$a& $a%at "eru(a& NAA &arena (tru&turnya (e$i&it #er#e$a+ /ehin''a NAA a&an "eran'(an' %e"an-an'an &*le*%til &ea"#ah -a'un'+ /e$an'&an &*le*%til yan' $iren$a" $ala" 2,4 D "enun-u&&an %e"an-an'an -arin'an le#ih (e$i&it $ari%a$a NAA $an AIA+ arena 2,4 D "eru%a&an at %en'atur tu"#uh, teta%i (tru&turnya #er#e$a $ari au&(in ala"i+ /ehin''a AIA *&(i$a(e ti$a& $a%at "eru(a& 2,4 D $an 2,4 D $a%at "eran'(an' %e"an-an'an -arin'an &*le*%til -a'un' $an &*le*%til yan' $iren$a" air (ulin' "enun-u&&an %erta"#ahan %an-an' rata@rata -arin'an+ !erta"#ahan rata@rata -arin'an $i(e#a#&an ter-a$inya %r*(e( *("*(i(+ !r*(e( *("*(i( ter-a$i &arena !O $an !A air (ulin' le#ih tin''i $ari%a$a !O $an !A -arin'an (ehin''a air #er%in$ah &e$ala" -arin'an+
D. D*&!$&*
?ela(&an #a'ai"ana %en'aruh #er#a'ai "aa" h*r"*ne tu"#uh terha$a% %e"an-an'an -arin'an a&ar $an #atan'+ /a"a&ah %en'aruhnya> e"u&a&an te*ri %en$u&un' yan' $a%at "en-ela(&an yan' $a%at "en-ela(&an ter-a$inya 'e-ala@'e-ala ter(e#ut+ ?aa#an:
BAB V PENUTUP A. S*%$a"
Ber$a(ar&an %ra&ti&u" yan' telah $ila&u&an, "a&a $a%at $i(i"%ul&an #aha %e"#erian h*r"*n au&(in "e"%en'aruhi -arin'an &*le*%til $an a&ar+ !a$a %eren$a"an -arin'an a&ar $an &*le*%til $ala" larutan 2,4 D (e#a'ai au&(in (inteti& $a%at "e"au le#ih e%at %a$a %erta"#ahan %an-an' -arin'an #ai& a&ar "au%un &*le*%til ea"#ah -a'un', -i&a $i#an$in'&an $en'an h*r"*ne NAA, AIA, $an air (ulin'+ Dan -u"lah larutan yan' a$a $i $ala" (el "enin'&at, &arena au&(in $a%at "enin'&at&an $i)u(i "a(u&nya air &e $ala" (el (ehin''a ter-a$i %e"an-an'an -arin'an yan' $ii&uti #erta"#ah %an-an'nya a&ar $an &*le*%til &ea"#ah -a'un'+ Be#era%a au&(in yan' $iha(il&an (eara ala"i *leh tu"#uhan, "i(alnya IAA In$*@leaeti Ai$5, !AA !henylaeti Ai$5 $an IBA In$*le#utri Ai$5+ Au&(in -u'a (u$ah $i%r*$u&(i (eara (inteti&, (e%erti NAA Na%thalene Aeti Ai$5 2,4 D $an 8!A 2@ 8ethyl@4 hl*r*%hen*6yaeti Ai$5+ B. Sara"
Dihara%&an &e%a$a %ra&ti&an untu& %ra&ti&u" (elan-utnya haru( le#ih teliti la'i $ala" "ela&u&an %er*#aan a'ar ha(il yan' $i%er*leh le#ih a&urat la'i+ /erta )a(ilita(@)a(ilita( yan' ter$a%at $i la#*rat*riu" $a%at $ita"#ah (ehin''a %ra&ti&u" $a%at #er-alan $en'an le#ih #ai& $an
e)i(ien+
DAFTAR PUSTAKA
Rahayu, Yuni /ri< Yuliani $an Lu&a( / Bu$i%ra"ana+ 2010+ Petunjuk Praktikum Fisiologi
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Tumbuhan+ /ura#aya: La#*rat*riu" .i(tu"@Bi*l*'i@Une(a+ • •
•
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La&itan, B+, 2004+ Dasar-Dasar Fisiologi Tumbuhan. !T Ra-a Gra)in$* !er(a$a, ?a&arta+ /u'ih(ant*(a, 2009+ Zat Pengatur tumbuh+ htt%:CC(u'ih(ant*(a+at(%ae+*"+ Dia&(e( /a#tu, 24 8ei 2014+ Y*6, 2007+ Zat Pengatur Tumbuh+ htt%:CC+en*+#l*'(%*t+*"+ Dia&(e( %a$a tan''al 24 8ei 2014+ /a("ita"ihar$-a, Dar$-at $an Ar#a(yah /ire'ar+ 199=+ Fisiologi Tumbuhan+ Ban$un': ITB !re((+
LAMPIRAN A. La%*ra" Pra!t*!$% F,t,
F,t,
Ketera"#a"
