Pemisahan antigen dan antisera

MCROZCH RZCEPLEÂ [NZOMODL

@lsusuh Omnf 9 GCPFLC H^ZFC[CHC 33 23 23 373 Enmcs @ Zndumnr [nanstnr WL Enmoapoe LLL

[NEOMCF PLHDDL LMA^ GCZAC[L JFCEPL RNZPLTL RCMNAJCHD :230

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RNZKOJCCH L RNAL[CFCH CHPL[NZC @CH CHPLDNH

3.3

Ojine Rrcetleua @crcf domohdch CJ

3.:

3.;

Puiuch Rrcetleua 

^htue anhdntcful kcrc pnalscfch chtldnh `ch chtlsnrc `cmca `crcf



^htue anhdntcful kcrc pnaurhlch nrltroslt



^htue anhdntcful kcrc pnaurhlch pmcsac

Mch`csch Pnorl @crcf c`cmcf kclrch ychd tnr`cpct pc`c snauc acefmue flùp (enkucml tuajufch)

tlhdect tlhddl ychd jnrguhdsl anhdlrlaech zct-zct `ch oesldnh ychd `ljutufech omnf icrlhdch tujuf, anhdchdeut jcfch-jcfch elalc fcslm antcjomlsan, `ch iudc snjcdcl pnrtcfchch tujuf tnrfc`cp vlrus ctcu jcetnrl. @crcf jnrwcrhc anrcf scapcl anrcf tnrchd cpcjlmc ecyc oesldnh `ch jnrwcrhc anrcf scapcl anrcf tuc cpcjlmc eneurchdch oesldnh. Tcrhc anrcf pc`c `crcf `lsnjcjech omnf fnaodmojlh, protnlh pnrhcpcsch (rnsplrctory protnlh) ychd anhdchùhd jnsl `cmca jnhtue fnan, ychd anrupcech tnapct tnrlecthyc aomneum-aomneum oesldnh. @crcf tnr`lrl ctcs ùc jcdlch ycltu pmcsac `crcf `ch snm `crcf. C`c tldc inhls snm `crcf, ycltu snm `crcf anrcf (nrltroslt), snm `crcf putlf (mnueoslt), `ch enplhd `crcf (troajoslt). 

[nm `crcf anrcf ctcu nrltroslt (sneltcr <<%)



Enplhd-enplhd `crcf ctcu troajoslt (2,7 - 3,2%)



[nm `crcf putlf ctcu mnueoslt (2,:%)







`crcf rnslplnh cech anhdceljctech rncesl-rncesl cmnrdl `ch ychd pcmlhd gctcm c`cmcf syoe chcglmcetle. C`c jnjnrcpc slstla pnhddomohdch `crcf, hcauh ychd tnrpnhtlhd uhtue tuiuchemlhls c`cmcf slstla pnhddomohdch `crcf CJO `ch Zfnsus. Anhurut slstla pnhddomohdch `crcf CJO, `crcf `ljcdl 0 domohdch, ycehl domohdch C, J, CJ `ch O5 uhtue pnhntcpch domohdch `crcf tnrsnjut `lduhcech rncdnh ychd `lsnjut chtlsnrc. Rmcsac `crcf (chtlsnrc) c`cmcf eoapohnh `crcf jnrjnhtue kclrch jnrwcrhc euhlhd ychd anhic`l an`lua snm-snm `crcf, `lachc snm `crcf `ltutup. >>% `crl iuamcf/vomuan `crcf anrupcech pmcsac `crcf. Womuan pmcsac `crcf tnr`lrl `crl <2% jnrupc clr `ch 32% jnrupc mcrutch protnlh, dmueosc, gcetor eocdumcsl, loh alhnrcm, foraoh `ch ecrjoh`loesl`c. Rmcsac `crcf (chtlsnrc) `cpct `lplscfech `l `cmca `cmc a snjucf tujc jnrlsl `crcf sndcr ychd tnmcf `ljujufl zct chtl-eocdumch ychd enau`lch `lputcr snhtrlgudcm scapcl snm `crcf anrcf ictuf en `cscr tujc, snm `crcf putlf cech jnrc`c `l ctcshyc `ch anajnhtue mcplsch juggy koct, pmcsac `crcf `cr cf jnrc`c `l ctcs ct cs mcplsch m cplsch tnrsnjut tn rsnjut `nhdch enpc`ctch sneltcr 32:> ed/a;, or 3.2:> ed/m. Chtldnh c`cmcf snjucf zct ychd anrchdschd rnspoh lauh, tnrutcac `cmca anhdfcslmech chtljo`l. Chtldnh jlcschyc jnrupc protnlh ctcu pomlscecrl`c,tntcpl `cpct iudc jnrupc aomneum mclhhyc, tnracsue aomneum enklm (fcptnh) ychd jnrdcjuhd `nhdch protnlh pnajcwc ctcu kcrrlnr. [pnslglecsl rncesl chtcrc chtldnh `ch chtljo`l tnmcf `ltuhiueech anmcmul pnhnmltlch pnhnmltlch ychd `lmceuech omnf Mch`stnlhnr. Lc anhddcjuhdech a nhddcjuhdech rc`lecm-rc`lecm ordchle pc`c protnlh `ch anhdfcslmech chtljo`l tnrfc`cp chtldnh-chtldnh tnrsnjut. Fcslm ychd `lpnromnf anhuhiueech chtljo`l `cpct anajn`cech chtcrc enmoapoe jnrjn`c pc`c protnlh ctcupuh euapumch elalc ychd scac tntcpl jnrjn`c enùùech. Zncesl chtcrc chtldnh `ch chtljo`l tnr`lrl `crl lectch hoh eovcmnh, (snpnrtl lectch fl`rodnh, vch `nr Tccms, nmnetrostctle, fl`rogojle), snflhddc rncesl lhl `cpct enajcml en snaumc (rnvnrsljmn). Eneuctch lectch lhl jnrdchtuhd enpc`c icrce chtcrc pcrctop `ch jcdlch-jcdlch jcdlch-jcdlch tnrtnhtu pc`c npltop.







3.>

Pchddcm

9 :0 Acrnt :230

Tcetu

9 2<.22 TLJ s/` snmnscl

Cmct `ch Jcfch Cmct Cmct ychd `lduhcech `cmca prcetleua c`cmcf 9 

Pcjuhd snhtrlgus



Kcwch porsnmlh



[nhtrlgus



[pult >kk



Rlpnt tntns





Pcjuhd rncesl



Zce tcjuhd rncesl

Ecpcs

Jcfch Jcfch ychd `lduhcech `cmca prcetleua c`cmcf 9 

@crcf domohdch CJ



Mcrutch HcKm glslomodls



Ecmslua emorl`c



Erlstcm caaohlua oescmct



3.7

Hctrlua czl`c Rrosnùr Enric

3.7.3

Rnalscfch Rmcsac (chtlsnrc) `ch Nrltroslt (chtldnh)



Cajlm `crcf > am, acsueech `cmca tcjuhd snhtrlgus.



[nhtrlgudcsl :222 rpa snmcac 32 anhlt.



Cajlm pmcsac `ch acsueech `cmca tcjuhd rncesl (chtlsnrc domohdch `crcf C )

3.7.: 

Rnaurhlch Nrltroslt (chtldnh) Nrltroslt pc`c tcjuhd snhtrlgus `l tcajcf `nhdch mcrutch HcKM glslomodls scac jchyce, cùe `nhdch kcrc anautcr autcrech tcjuhd snhtrlgus s nhtrlgus pc`c enùc tnmcpce tchdch.



[nhtrlgudcsl :22 rpa snmcac 32 anhlt.









Mceuech prosnùr lhl scapcl ; ecml, snflhddc `lpnromnf nrltroslt jnrslf( nrltroslt lhl `lchddcp 322 %).

3.7.; 

Rnaurhlch Rmcsac (chtlsnrc) Kclrch pmcsac `ltcajcfech erlstcm ecmslua emorl`c snjchyce 3 ad uhtue 3 am, cùe, jlcrech 32 anhlt.



[crlhd `nhdch ecpcs, mcmu `ltcajcfech mcdl ecmslua emorl`c snjchyce 3 ad uhtue 3 am `crcf, cùe, jlcrech 32 anhlt.



Mceuech snjchyce ; ecml



Enau`lch `ltcajcfech `nhdch erlstcm caaohlua oescmct snjchyce 3 ad uhtue 3 am `crcf, cùe, jlcrech 32 anhlt, enau`lch scrlhd.

3.4



Mceuech pnhdnricch lhl snjchyce ; ecml



@ltcajcfech hctrlua czl`c snjchyce 3 ad uhtue 3 am `crcf



Chtlsnrc slcp `lduhcech.

Fcslm `ch Rnajcfcsch 3.4.3

Fcslm

@crcf Domohdch CJ

@crcf domohdch CJ ychd tnmcf `lsnhtrlgudcsl, tnric`l pnalscfch chtcrc pmcsac `crcf `ch nrltroslt







Rmcsac aurhl domohdch CJ

Nrltroslt aurhl domohdch CJ

[upnrhctch domohdch CJ

3.4.:

Rnajcfcsch Rc`c prcetleua ecml lhl `lmceuech pnalscfch chtldnh `ch chtlsnrc `nhdch

anhdduhcech cmct snhtrlgus. Rrlhslp enric `crl cmct lhl ycltu `lachc ojine `lputcr snkcrc forlzohtcm pc`c icrce rc`lcm `crl tltle, `lachc `ltltle tnrsnjut `lenhcech dcyc. Rc`c scct ojine `lputcr, pcrtlenm-pcrtlenm ychd c`c cech jnrplscf `ch jnrpnhkcr snsucl jnrct inhls acslhd-acslhd pcrtlenm. @nhdch dcyc ychd pcmlhd jnrpnrch c`cmcf dcyc snhtrlgudcm. [capnm `lacsueech en`cmca tcjuhd snhtrlgus enau`lch `lsnhtrlgudcsl `nhdch enknpctch :222rpa snmcac 32 anhlt `ch sntnmcf scapnm `lsnhtrlgudcsl cech tnric`l pnalscfch jnr`cscrech jnrct inhls, `lachc mcplsch pcmlhd jcwcf c`cmcf nrltroslt (chtldnh) `ch mcplsch ctcs c`cmcf pmcsac `crcf (chtlsnrc).









ecmslua emorl`c c`cmcf uhtue anhdlect ecmslua ychd tnr`cpct pc`c chtlsnrc snflhddc `lpnromnf chtlsnrc ychd aurhl. Rc`c nrltroslt `ltcajcfech HcKm glslomodls scac jchyce enau`lch `lsnhtrlgudcsl snjchyce ;x. Puiuch pnhcajcfch HcKm glslomodls c`cmcf uhtue analscfech nrltroslt `crl pnhdotorhyc snflhddc `lpnromnf `lpnromnf nrltroslt (chtldnh) ychd aurhl.

3.6

Enslapumch @crl fcslm pnrkojcch pnalscfch chtlsnrc `ch chtldnh `l`cpct ùc mcplsch, mcplsch jnhlhd jnrwcrhc eneuhlhdch ycltu chtlsnrc `ch ychd jnrwcrhc anrcf ycltu nrltroslt ctcu chtldnh.







@CGPCZ R^[PCEC

Altsueorcyhzz. Altsueorcyhzz. ” pnanrlescch chtlsnrc `ch chtldnh‑. chtldnh‑. 33 @nsnajnr :233. fttp9//altsueorcyhzz.wor`prnss.koa/:233/3:/33/pnanrlescch-chtlsnrc-`ch-chtldnh/

[nhtrlgudcsl. ”snhtrlgudcsl‑. :2 [nptnajnr :23: fttp9//snhtrlgudcsl:23:.jmodspot.koa/:23:/2
Tlelpn`lc ”@crcf‑. fttp9//l`.wlelpn`lc.ord/wlel/@crcf







RNZKOJCCH LL RNANZLE[CCH [RN[LGL[LPC[ CHPL[NZC

:.3

Ojine Rrcetleua Nrltroslt aurhl domohdch CJ

:.:

Puiuch Rrcetleua ^htue anhdntcful kcrc pnanrlescch spnslglsltcs chtlsnrc

:.;

Mch`csch Pnorl Chtlsnrua c`cmcf snrua `crl achuslc ctcu fnwch ychd anhdchùhd chtljo`y uhtue

anmcwch jnrjcdcl ackca pnhycelt ychd spnslgle `ch jlcschych anajnrlech lauhltcs pcslg enpc`c pnhycelt tnrsnjut. Jcdlch pmcsac `crcf (chtlsnrua) ychd anapuhycl guhdsl pnhtlhd c`cmcf snrua. [nrua anrupcech pmcsac `crcf ychd `lenmucrech ctcu `lplscfech gljrlhodnhhyc `nhdch kcrc anautcr `crcf `cmca snhtrlgudn. [nrua tcapce schdct inrhlf `ch anhdchùhd zct chtljo`l. Chtljo`l lhl jnrguhdsl uhtue anajlhcscech protnlh cslhd ychd acsue en `cmca tujuf. Rrotnlh cslhd ychd acsue en `cmca tujuf `lsnjut chtldnh. @crcf `ljcdl `cmca jnrjcdcl domohdch jnr`cscrech tlpn chtldnh ychd tnr`cpct `l`cmca snm.. Anajrch nrltroslt anhdchùhd ùc chtldnh ycltu tlpn-C `ch tlpn-J. chtldnh lhl









Cdmutlhodnh-CJ 9 domohdch ychd analmlel enùc inhls nhzla tnrsnjut. Cfml lauhomodl (lmau tnhtchd enenjcmch tujuf) enjchdscch Custrlc jnrhcac Ecrm

Mch`stnlhnr (3676-3<0;) anhdnmoapoeech domohdch `crcf achuslc.

Jnr`cscrech

”c`c ctcu

tl`ce c`chyc cdmutlhodnh‑ acec acec domohdch `crcf `lenmoapoeech anhic`l domohdch `crcf C,

J, CJ, `ch O. 

Domohdch `crcf C, ycltu ilec nrltroslt anhdchùhd cdmutlhodnh-C `ch cdmutlhlh-j `cmca pmcsac `crcf.



Domohdch `crcf J, ycltu ilec nrltroslt anhdchùhd cdmutlhodnh-J `ch cdmutlhlh-c `cmca pmcsc `crcf.



Domohdch `crcf CJ, ycltu ilec nrltroslt anhdchùhd cdmutlhodnh-C `ch J, `ch pmcsac `crcf tl`ce anlmlel cdmutlhlh.



Domohdch `crcf O, ycltu ilec nrltroslt tl`ce analmlel cdutlhodnh-C `ch J, `ch pmcsac `crcf analmlel cdmutlhlh-c `ch j.

Pcjnm Domohdch @crcf Jnr`cscrech Cdmutlhlh `ch Cdmutlhodnh

Domohdch @crcf

Cdmutlhodnh

Cdmutlhlh

C

C

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J

J

c

CJ

C `ch J

Pl`ce C`c







Tcetu

:.>

9 2<.22 TLJ s/` snmnscl

Cmct `ch Jcfch Cmct Cmct ychd `lduhcech `cmca prcetleua c`cmcf 9 

Rlpnt tntns



Pcjuhd rncesl



Zce tcjuhd rncesl



Ojine dmcss

Jcfch Jcfch ychd `lduhcech `cmca prcetleua c`cmcf 9

:.7



Rmcsac aurhl domohdch CJ



Nrltroslt >% domohdch C, J, CJ `ch O



Mcrutch HcKm glslomodls

Rrosnùr Enric :.7.3

Rnajuctch Nrltroslt >%









:.7.: 

îl [pnslgltcs Chtlsnrc Pntnsech `lctcs 0 jucf ojine dmcss jnrslf mcrutch chtlsnrc (pmcsac domohdch C ychd tnmcf `laurhlech) acslhd-acslhd snjchyce 3 tntns



Rc`c ojine dmcss pnrtcac `ltcajcfech 3 tntns nrltroslt >% domohdch C, mcmu cactl rncesl ychd tnric`l



Rc`c ojine dmcss enùc `ltcajcfech 3 tntns nrltroslt >% domohdch J, mcmu cactl rncesl ychd tnric`l



Rc`c ojine dmcss entldc tcajcfech 3 tntns nrltroslt > % domohdch CJ , mcmu cactl rncesl ychd tnric`l



@ch pc`c ojine dmcss ennapct `ltcajcfech 3 tntns nrltroslt >% domohdch O, mcmu cactl rncesl ychd tnric`l



Rnhdnricch ychd scac iudc `lmceuech tnrfc`cp pmcsac domohdch J, CJ, `ch O



Pcjnmech fcslm rncesl ychd tnric`l, jlmc tnric`l cdmutlhcsl `lhyctcech `nhdch tch`c (+), `ch jlmc rncesl hndctlg `lhyctcech `nhdch tch`c hndctlg ( -)

:.4

Fcslm `ch Rnajcfcsch :.4.3

Fcslm







Domohdch @crcf

Rmcsac `crcf CJ

Domohdch C

(-)

Domohdch J

(-)

Domohdch CJ

(-)

Domohdch O

(-)

:.4.:

Rnajcfcsch Rc`c pnrkojcch lhl pmcsac CJ tl`ce anhuhiueech pnhdduapcmch entlec

`ljnrlech nrltroslt C, J, CJ, `ch O. Domohdch `crcf CJ ychd tl`ce analmlel cdmutlhlh C `ch cdmutlhlh J, `cpct jnrslgct rnklplnht uhlvnrscm, `lachc domohdch lhl fchyc acapu anhnrlac `crcf ychd `ltrchsguslech `crl jnrjcdcl domohdch `crcf tnracsue domohdch `crcf CJ ltu snh`lrl. Ecrnhc domohdch lhl anapuhycl ùc ackca cdmutlhodnh (C&J), acec entlec `crcf `crl domohdch mclh (C,J, `ch O) `ltrchsguslech en domohdch lhl, pc`c scct ltu domohdch CJ cech anapnrtnauech sctu ackca cdmutlhodnh (alscmhyc J) ychd







@CGPCZ R^[PCEC Chhc, ”Rnhddomohdch @crcf‑, ; @nsnajnr :23:. fttp9//chhc-chcchc.jmodspot.koa/:23:/3:/domohdch-`crcf.ftam

Jlomodl Mncrhlhd Knhtnr, ”DOMOHDCH ” DOMOHDCH @CZCF @CH PZCH[G^[L @CZCF ‑. ;3 Ichucrl :23:. fttp9//jlomodymncrhlhdknhtnr.jmodspot.koa/p/pnrtnauch-:.ftam :23:. fttp9//jlomodymncrhlhdknhtnr.jmodspot.koa/p/pnrtnauch-:.ftam

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Pemisahan antigen dan antisera

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