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I N ST R RU UCTION MANUA L
Labomed , Inc.,2921.La Cienega Blvd.,Suite A.Culver City,CA 90232U.S.A. TEL(0310)202-0814 FAX:(0310)202-7286
LABOMED
SPECTRO DOUBLE AND DUAL SPLIT BEAM -------------------------------------------------------------------UVS – 2700 | UVS – 2800 UVD-2950 | UVD-2960 | UVD-3000 | UVD-3200 | UVD-3500
Labomed , Inc.,2921.La Cienega Blvd.,Suite A.Culver City,CA 90232U.S.A. TEL(0310)202-0814 FAX:(0310)202-7286
Instruction manual
CONTENT Chapter 1
Installation………………………………………………………………………………………..1-1 Installation………………………………………… ……………………………………………..1-1
1.1
Inspection of Parts………………………………………………………………………………1-1
1.2
General Description of the Spectrophotometer………………………………………………………1-1 1.2.1 Front side of the instrument……………………………………………………………………1-1 1.2.2 Rear side of the instrument…………………………………………………………………….…1-2 1.2.3 Display……………………………………………………………………………………………1-2 1.2.4 Keyboard………………………………………………………………………………………….1-3
1.3
Site Require ment…………………………………………………………………… ment…………………………………………………………………………………….…1-4 ……………….…1-4
1.4
Installation…………………………………………………………………………………………...…1-4
1.5
Turning on the instrument…………………………………………………………………………...…1-4
1.6
Performance Check at Installation……………………………………………………………………..1-5 1.6.1 Wavelength accuracy and reproducibility…...……………………………………………………1-5 1.6.2 Baseline flatness…………………………..………………………………………………………1-5
Chapter 2 Instrument Specifications……………………………………………………………………………2-1 Specifications……………………………………………………………………………2-1 Chapter 3 Photometric Measurement……………………………………………………………………………3-1 Measurement……………………………………………………………………………3-1 3.1
Information Display and Function Keys………………………………………………………………3-1
3.2
Parameter Setting………………………………………………………………………………3-1 3.2.1 Setting photometric mode………………………………………………………………………3-2 3.2.2 Setting measuring wavelength………………………………………………………………3-2 3.2.3 Inputting K-Factor……………………………………………………………………………3-3 3.2.4 Quitting parameter setting………………………………………………………………………3-4
3.3
Setting working wavelength………………………………………………………………………3-4
3.4
Auto-zeroing……………………………………………………………………………………………3-4
3.5
Sample Con trol…………………………………………………………………… trol…………………………………………………………………………………………3-4 ……………………3-4 3.5.1 Selecting sample holder type………………………………………………………………….…3-5 3.5.2 Setting sample cell number in use……..…………………………………………..…3-5 3.5.3 Cell one blank correction……………………………………………………………….…3-6 3.5.4 Sample cell shifting (Move Cell) ………………………………………………………………3-6 3.5.5 Resetting to cell No.1 (Move Home) ……………………………………………………………3-6 3.5.6 Single-cell measurement …..………………………………………………………………3-6 3.5.7 Quitting sample control…………………………………………………………………………3-6
3.6
Measuring Operation…………………………………………………………………………………...3-6 3.6.1 Setting measuring wavelength……………………………………………………………………3-7 3.6.2 Auto zeroing…………………………………………………………………………… zero ing………………………………………………………………………………………3-7 …………3-7 3.6.3 Starting measurement……………………………………………………………………………..3-7 3.6.4 Looking up the measured results………………………………………………………………….3-8 3.6.5 Clearing off the measured data……………………………………………………...……………3-8 3.6.6 Printing o ut…………………………………………………………………… ut…………………………………………………………………………..……………3-8 ……..……………3-8 3.6.7 Quitting measuring operation……………………………………………………………………..3-9
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Chapter 4 Spectrum Scanning………………………………………………………………………………4-1 Scanning………………………………………………………………………………4-1 4.1
Information Display and Function Keys……………………………………………………………….4-1
4.2
Parameter Setting………………………………………………………………………………………4-1 4.2.1 Setting photometric mode………………………………………………………………………4-2 4.2.2 Setting scanning speed……………………………………………………………………………4-2 4.2.3 Setting sampling interval………………………………………………………………….……..4-3 4.2.4 Inputting wavelength rang…………………………………………………………………..4-3 4.2.5 Inputting ordinate scale range………………………………………………………………4-3 4.2.6 Dark current correction………………………………………………………………………4-3 4.2.7 Quitting parameter settings………………………………………………………………………4-3
4.3
Setting current wavelength………………………………………………………………………4-3
4.4
Baseline Correction…………………………………………………………………………………….4-4
4.5
Measuring Operation………………………………………………………………………………4-4
4.6
Peak-picking………………………………………………………………………………4-5
4.7
Printing Out Measured Results……………………………………………………………………4-7
4.8
Quitting Spectrum Scanning Measurement……………………………………………………………4-7
Chapter 5 Quantitative Measurement ………………….………………………………………………………5-1 5.1
Information Display and Function Keys………………………………………………………………5-1
5.2
Parameter Setting………………………………………………………………………………5-1 5.2.1 Setting measurement method……………………………………………………………………5-2 5.2.2 Setting measuring wavelength…………………………………………………………………5-2 5.2.3 Setting concentration unit………………………………………………………………………5-2 5.2.4 Setting calibration curve parameters for K-Factor method………………………………………5-3 5.2.4.1 Setting the slope of calibration curve……………………………………………………5-4 5.2.4.2 Setting the intercept of calibration curve…………………………………………………5-4 5.2.4.3 Quitting calibration curve parameter setting………………………………………………5-5 5.2.5 Setting calibration curve parameters for Calibration Curve method……………………………5-5 5.2.5.1 Setting the number of standards…………………………………………………………5-5 5.2.5.2 Entering standard sample concentrations and absorbance………………………………5-6 5.2.5.3 Starting standard sample measurement…………………………………………………5-7 5.2.5.4 Building the calibration curve……………………………………………………………5-7 5.2.5.5 Printing out parameters of the calibration curve…………………………………………5-8 5.2.5.6 Quitting calibration curve parameter setting………………………………………………5-8 5.2.6 Quitting parameter setting of quantitative measurement…………………………………………5-8
5.3
Auto Zeroin g……………………………………………………………………… g……………………………………………………………………………………...…5-8 ……………...…5-8
5.4
Setting current wavelength………………………………………………………………………5-8
5.5
Measuring Operation………………………………………………………………………………5-10
5.6
Looking Up the Measured Results……………………………………………………………………5-11
5.7
Printing Out…………………………………………………………………… Out………………………………………………………………………………………..5-11 …………………..5-11
5.8
Quitting Quantitative Measurement…………………………………………………………………5-11
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Chapter 6 DNA/Protein DNA/Protein Analysis………………………………………………………………………………6-1 6.1
Information Display and Function Keys………………………………………………………
……6-1
6.2
Setting Parameters……………………………………………………………………………………..6-1 6.2.1 Setting DNA/Protein analysis method…………………………………………………… ………6-2 6.2.2 Setting measuring wavelength…………………………………………………………………….6-2 6.2.3 Setting DNA factor……….……………………………………………………………………….6-3 6.2.4 Setting Protein factor…………………………………………………………………… ………..6-3 6.2.5 Setting background calibration at 320nm…………………………………………………………6-3 6.2.6 Setting calibration factor…………………………………………………………………………..6-4
6.3
Quitting Parameter Setting of DNA/Protein…………………………………………………… ………6-4
6.4
Auto Zeroing …………………………………………………………………… …………………………………………………………………………………………….6-4 ……………………….6-4
6.5
Measuring Operation…………………………………………………………………………… ………6-4
6.6
Looking Up The Measured Results……………….…………………………………………… ………6-4
6.7
Printing Out……………………………………………………………….…………………… ………6-4
6.8
Quitting Measurement………………………………………………………………………… ………6-4
Chapter 7 Utilities…………………………………………………………… Utilities………………………………………………………………………………7-1 …………………7-1 7.1
Information Display and Function Keys…………………….………………………...………………...7-1
7.2
Setting Lamp Changeover Wavelength………………………………………………………………….7-1
7.3
Setting Spectral Bandwidth………………………………………………………………………… Bandwidth……………………………………………………………………………...7-2 …...7-2
7.4
Setting W Lamp On/Off………………………………………………………… On/Off…………………………………………………………………………………7-2 ………………………7-2
7.5
Setting D2 Lamp On/Off………………………………………………… On/Off………………………………………………………………………………...7-2 ……………………………...7-2
7.6
Setting time……………………………………..…………………………… time……………………………………..……………………………………………………….7-3 ………………………….7-3
7.7
Dark Current Adjustment……………………………………………………………………………… Adjustment………………………………………………………………………………..7-3 ..7-3
7.8
Wavelength Calibration (Wavelength (Wavelength Adj)……………………………………………………………...7-3
7.9
Quitting Utilities Mode………………………………………………………………………………… Mode………………………………………………………………………………….7-3 .7-3
Chapter 8 Maintenance……………… Maintenance………………………………………………………………………………8 ………………………………………………………………8-1 -1 8.1
Points for attention………………………………………………………………………………………8-1
8.2
Daily Care………………………………………………………………………… Care……………………………………………………………………………………………….8-1 …………………….8-1
8.3
Trouble shooting and maintenance……………………………………………………………………...8-1 8.3.1 Diagnoses of initialization…………………………………………………………………………8-1
8.4
Light Source Replacement……………………………………………………………………………..8-3 8.4.1 Replacement of the W lamp……………………………………………………………………….8-3 8.4.2 Replacement of the D2 lamp………………………………………………………………………8-3
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Chapter1 Installation 1.1 Inspection of Parts When unpacking, confirm that all the items listed are included.
1.2 General Description of the Spectrophotometer Spectrophotometer 1.2.1 Front side of the instrument instrument (1) LCD display The 320×240 dots high resolution LCD, which allows users to adjust the brightness (the brightness adjustor is on the top left corner of the keyboard) , is used to display measurement information, parameters, data and spectrograms. (2) Keyboard The 27 soft-touch keys on the keyboard are used for instrument control and operation. (3) Sample compartment Automatic 8-cell holder to hold the samples.
1.2.2 Back side of the instrument instrument (1)
Light source housing The halogen lamp (for the visible region) and the deuterium lamp (for the ultraviolet region) are installed in the light source housing. The lamps switch over automatically according to the wavelength range while measuring.
The lamps will turn hot when they are lit. Be careful not to be burnt when lamp replacement is
made.
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(2)
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Printer interface Used to connect with HP 600 series of jet printer.
(3)
RS-232 interface RS-232 interface is for serial communication.
(4)
Power Switch AC power outlet is used to connect to the 110-240V, 50-60Hz main power. Fuse holder is to hold a 1A-fuse tube of 5mm. Power switch is to turn on/off the power of the instrument.
(5)
Light source housing cover It is for access to the light source for the replacement. Be careful not to be burned by the heated lamps when replacing the light sources.
(6) Ventilating fan The opening of the fan is the outlet for ventilation and dissipating heat. Please place the main unit at least 20-30cm away from the wall in case that the outlet is blocked.
1.2.3 Display The display of Spectro Dual Split Beam is the 320×240 dots high resolution LCD that can not only display the measurement data, parameters, mode and man-machine interaction information, but also the spectrum measured. The display is shown as follows: Indicating Area For
Mode Line
Function Keys
Information Indicating Area
Operating
Time
Area
The first line is the mode line, which is used to indicate current working mode. The system provides four working modes:
Photometric Measurement – Used to make measurement at fixed wavelengths. K-Factor calculation is possible.
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Spectrum Measurement – Used to provide a wavelength scan and data processing in the set wavelength range.
Quantitative measurement – Used to provide one wavelength quantitative determination and data processing.
DNA/Protein analysis – Used to provide 3 measurement methods for the analysis of DNA/Protein.
Utilities – Used to set the parameters for the instrument system.
The column on the right of the screen is the indicating area for function keys (F1~F4). The function of each function key may vary from page to page. In the middle of the screen is the operating area. At the bottom of the screen is the information indicating area, which allows you to enter parameter values there. 1.2.4 Keyboard There are altogether 27 soft-touch keys on the keyboard of Spectro Dual Split Beam, which are divided into 4 groups. The functions of the keys are described as follows:
(1) Function keys (F1~F5) The function keys are used to set instrument parameters and to perform operation functions. When one of the four function keys (F1~F5) is pressed in different working mode, the system executes the corresponding operation, such as parameter setting, sample cell setting and printing etc.. (2) Numeric keys (0~9, “.” and “-”) Used to enter a numerical value in parameter setting. (3) Edit keys ( ▲, ▼,
,
CE, ENTER, RETURN)
Cursor keys (▲,▼): Used to shift the cursor up and down while browsing data and increase or decrease data while setting time.
Cursor keys (
,
,
): Used to switch the displayed data while measuring DNA/Protein and switch
the objects while setting time.
Cancel key [CE]: Used to clear off the preceding entries or cancel a certain operation.
Enter key [ENTER]: Used to confirm and execute the current function, or to end the parameter setting and quit.
Return key [RETURN]: Used to quit the current function and return to the preceding page.
(4) Operation keys (AUTOZERO, GOλ, START/STOP)
Auto zero key [AUTOZERO]: Used to set ABS zero (transmittance 100%). In photometric mode and the quantitative mode, [AUTOZERO] set ABS zero only at current wavelength. In spectrum mode, [AUTOZERO] performs baseline calibration in the set wavelength range.
Wavelength set key [GOλ]: Used to set the current wavelength.
Start/Stop key [S TART/STOP]: Used to start or stop a measurement.
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1.3 Site Requirement
To ensure a better working quality and to prolong the service life of the instrument, install the instrument in a place that satisfies the following requirements: a.
Ambient temperature: 15℃~35℃
b.
Relative humidity:
c.
The instrument should be placed on a stable workbench, avoiding strong vibrations.
d.
Avoid a place with strong magnetic or electric fields and corrosive gases.
e.
Power requirements:
less than 80%
AC110V±10%, 60Hz±1Hz, single phase. A regulated power supply with at
least 500VA capacity is recommended. There should be a ground line in the laboratory to ensure the instrument to be well grounded. 1.4 Installation Spectro Dual Split Beam Spectrophotometer consists of the spectrophotometer main unit and a HP jet-printer (optional). The connections between them are shown in the following figure. Item (1) and (2) are the power cords which should not be connected to the main power until other connections are made, to avoid instrument damage. Item (3) is the printing cable, one end of which with a 25-pin plug is connected to the main unit, the other end with a 36-pin plug is connected to the printer. Connect all the cables firmly by locking the screw (if there is any) on the plugs. After checking to ensure that the power supply is AC220V, 50Hz, plug in the power cords to connect the spectrophotometer and the printer to the main power. Note: The spectrophotometer is a precision electronic instrument. Don’t pull out or insert the power cable or tie cable when the instrument is on, otherwise it will destroy the instrument and the computer. computer.
1.5 Turning on the Instrument After confirming that there is no light shield in the sample compartment, turn on the power switch of the main unit and then the printer (if there is). The initializing page will be displayed on the LCD. The instrument starts self-checking and initializing. This process will take about 3 minutes. After initializing, the system displays the instrument main menu.
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Users can select a working mode by depressing the corresponding numeric keys (1-5). It usually takes 15~30 minutes for the instrument to warm up before measurement can be started. After measurement, you should first turn off the main unit, then the printer, and at last other equipment (such as the regulated power supply).
1.6 Performance Check at Installation After finishing the installation, check each of the following items. Periodic checking should also be made during operation. Proceed the performance check according to the following procedure 30 minutes after turning on the power switch. 1.6.1
Wavelength accuracy and reproducibility
The wavelength accuracy should be within ±0.5nm. The wavelength reproducibility should be within 0.2nm.
Check with the two spectral lines of the D2 lamp according to the following procedure. 0)
Confirm the spectral bandwidth is set to 2nm. The initialized spectral bandwidth is 2nm. Please refer to THE UTILITIES FUNCTION for details of setting the spectral bandwidth.
1)
Press [2] key to select mode “Spectrum Measurement” on the main menu.
2)
In Abs or %T mode, press [GOλ] to set the wavelength to 330nm, to turn to the D2 lamp working mode.
3)
Press [F1] key to select parameter setting function. Set the following parameters: Sampling interval: 0.2nm; Scanning speed: middle; Wavelength range: 660~480nm; Recording range: 0~100; Mode: Es.
4)
Press [RETURN] key and set the energy gain value 1-100 to 5~7, to start scanning.
5)
Press [F2] key and set the min. peak/valley value to 1~10, to perform the peak-picking. Press [F4] to print out.
Press [RETURN] scanning for three times and perform peak-picking and printing out. Compare the picked wavelengths with the standard wavelengths of the two peaks of D2 lamp: 656.1nm and 486.0nm. 1.6.2
Baseline flatness
With air in sample cell, follow the procedure below to measure the Abs value at whole wavelength range in spectrum mode:
0)
Confirm that spectral bandwidth is set to 2nm. The initialized spectral bandwidth is 2nm. Please refer to THE UTILITIES FUNTION for details of setting the spectral bandwidth.
1)
Press [2] key to select mode “Spectrum Measurement” on the main menu.
2)
Press [F1] key to select parameter set function. Set the following parameters: Sampling interval: 1nm; Scanning speed: middle; (Scan WL): 1100~190nm; (Range): -0.1~0.1; Photometric mode: Abs.
1-5
3)
Press [RETURN] to start baseline correction.
4)
Press [START/STOP] to start scanning.
5)
Press [F4] key to print out the spectrum.
Instruction manual
Compare the max. Abs value on the spectrum with the value required for the baseline flatness. (Sudden change in Abs value is allowed at the point of light source changeover.)
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Chapter2 Instrument Specifications
Model
Spectro Dual Split Beam UVS-2700
Wavelength range
Spectro Dual Split Beam UVS-2800
190nm~1100nm
Spectral bandwidth
2nm
5, 2, 1, 0.5nm (4 steps)
Resolution
2nm
0.5nm
Wavelength display
0.1nm
Wavelength settin g
1nm increment for scan starting and ending wavelengths setting. 0.1nm increment for GOTO-λsetting.
Wavelength accura cy
±0.5nm (with automatic wavelength correction)
Wavelength reproducibility
±0.2nm
Photometric system
Double beam ratio monitoring system
Photometric accuracy
±0.002Abs (0~0.5Abs) ±0.004Abs (0.5~1.0Abs) ±0.3%T (0~100%T)
Photometric reproducibility
±0.001Abs (0~0.5Abs) ±0.002Abs (0.5~1.0Abs) ±0.15%T (0~100%T)
Stray light
≤0.3%T (220nm)
100%T Noisy
≤0.3%T(at 500nm, 2nm bandwidth, for 2 minutes)
Baseline flatness
±0.004Abs (2nm bandwidth , middle scanning ,1100~190nm)
Stability
≤0.002A/30min (2 hours warming up, 2nm bandwidth, at 500nm)
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Chapter3 Photometric Measurement In photometric mode, one-wavelength measurements of absorbance and transmittance can be made. K-Factor calculation and printing out are possible.
3.1 Information Display and Function Keys On the instrument main menu , press [1] key to select photometric measurement mode. The screen of photometric mode is displayed on LCD, as shown in Fig. (3-1).
Fig.(3-1) The working picture of photometric measurement measurement The first line on the menu indicates that the current working mode is photometric mode. In the operating area displays the current measuring wavelength (660.0nm) and the measured value (0.001Abs). On the right, the functions of F1-F4 keys are displayed. [F1] - parameter setting [F2] – deleting the measurement data and refresh the screen [F3] –setting cell holder [F4] - printing the measurement results On this page, you can also set the wavelength and execute auto-zero by depressing [GOλ] key and [AUTOZERO] key respectively.
3.2 Parameter Setting On the working picture of photometric measurement, press [F1] key to display the parameter setting page, as shown in Fig.(3-2).
Fig. (3-2)
Parameter setting page of photometric measurement measurement 1
Instruction manual
On this page, users can set the photometric mode, wavelength and the value of factor K by pressing the corresponding item No.. After finishing parameter setting, press [RETURN] key to return to the preceding page. 3.2.1 Setting photometric photometric mode mode Press [1] key to set photometric mode. Two modes are available: Abs and %T. They can be selected alternately each time when [1] key is pressed.
Fig.(3-3) Mode setting page in photometric measurement measurement 3.2.2Setting Measuring Wavelength Wavelength Press [2] key to display the measuring wavelength setting page in photometric measurement. The indicating message indicates users to input the measuring wavelength, as shown in Fig. (3-4).
Fig. (3-4) Wavelength Wavelength setting page page in photometric photometric measurement measurement At the bottom of this page, enter desired wavelength value by pressing the numeric keys (0~9) and decimal point (.). The CE key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit this page directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. The input value keeps one-digit floating number after the decimal point If the figure entered exceeds five digits, the system will delete what has been entered automatically and you should start a new entry again. 3.2.3 Setting K-Factor Press [3] key to display K-factor setting page (Fig. 3-5) in photometric measurement. The indicating line at the bottom of the page indicates users to enter factor K value.
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Fig. (3-5) K-Factor setting setting page in photometric photometric measurement measurement At the bottom of the page, set the K-Factor value by pressing the numeric keys (0~9), [.] and [-] key. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit this page directly. A 4-digit figure (0~±9999) entry is allowed. If the figure entered exceeds four digits, the system will delete what has been entered automatically. You should start a new entry. 3.2.4 Quitting parameter parameter setting Press [RETURN] key to quit parameter setting and return to the main menu of photometric measurement.
3.3 Setting Working Wavelength On the main menu of photometric measurement, press [GOTO-λ] key to display the following page (Fig. 3-6) and set the working wavelength for photometric measurement.
Fig. (3-6) Wavelength Wavelength setting page page in photometric photometric measurement measurement At the bottom of this page, enter the wavelength value by pressing the numeric keys (0~9) and decimal point (.). The CE key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit this page directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds five digits, the system will delete what has been entered automatically and you should start a new entry again.
3.4 Auto-zeroing (100%T setting) On the main menu of photometric measurement, press [AUTOZERO] key to set Abs zero automatically at the current measuring wavelength. Before starting auto-zeroing, put the blank sample into the sample compartment.
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3.5 Sample Control On the main menu of photometric measurement, press [F3] key to display the sample control page as shown in Fig. (3-7).
Fig. (3-7) Sample control control page in photometric photometric measurement measurement Users can select sample cell-holder type (fixed cell-holder, 5-cell holder and 8-cell holder) in the sample control page. If the 8-cell holder is selected, you can set the working parameters of the 8-cell holder by pressing the corresponding numeric keys. After finishing the parameter setting, press [RETURN] key to return to the preceding page. 3.5.1 Selecting sample sample module type Press [1] key to select sample module type. There are three types available, fixed cell-holder, 5-cell holder and 8-cell holder, which can be selected alternately each time [1] key is pressed. 3.5.2 Setting sample cell number number in use (Drive Cell Cell No.) This parameter is only available to 5-cell holder and 8-cell holder. Press [2] key to enter the setting page (as shown in Fig.3-8). The system will indicate you to enter the number of sample cell in use (Drive Cell No.). At the bottom of this page, enter the sample cell number by pressing the numeric keys (0~9). [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. A 1-digit figure entry is allowed. The range is from 1 to 8. If the entered figure exceeds the range, the system will delete what has been entered. You should start a new entry. In the process of measurement, the system will measure the samples in the set sample cells one by one. The serial numbers of the sample cells are expressed by n-1, n-2,….
Fig. (3-8) Sample cell cell number setting page
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3.5.3No. 1 Cell blank correction This parameter is used to set No. 1 cell blank calibration function which means to calibrate the measuring result of the sample in each sample cell (No. 2-8) with that of the blank in the No. 1 cell. In measurement, the system will deduct the measured value of the blank in No. 1 cell from that of the samples in the same cell (No.2 ~ No.8), the result of which will be the measuring result. Two choices are available for this item: YES and NO, which alternate with each other each time [3] key is pressed. 3.5.4 Sample cell cell shifting (Move Cell) Press [4] key to shift the cell holder to the next sample cell. 3.5.5 Resetting to cell cell No.1(Move Home) Home) Press [5] key to reset the cell holder to sample cell No.1. 3.5.6 Single-cell measurement If the single-cell type is selected, you can choose any of the cells of the multi-cell as the measuring cell by shifting the cell holder to the desired position according to the method described in 3.5.6. Measurement will only be done to the chosen cell. 3.5.7 Quitting sample sample control control When sample control operation is finished, users can press [RETURN] key to quit sample control function and return to the main menu of photometric measurement.
3.6 Measuring Operation On the main menu of photometric measurement, press [START] key to start the measurement (Fig. 3-9).
Fig. (3-9) Operating page page of photometric measurement measurement In the table shown on the screen, “NO.” means the sample No. set by users, which increases by one after each measurement. “Abs” is the measured value Column. “K·Abs” is the value of the measurement multiplied by K-Factor. In this page, press [START/STOP] key to start measurement. Press [F2] key to delete all the measuring data. Press [F4] to print out the measuring data. After finishing all the measurement operation, press [RETURN] key to return to the main menu of operation. 3.6.1
Setting measuring wavelength
Press [GOλ] key to set measuring wavelength. Please see Section 3.3 for the details of measuring wavelength setting.
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3.6.2
Instruction manual
Auto zeroing (100%T calibration)
Press [AUTOZERO] key to set Abs zero at the current wavelength. Please see Section 3.4 for the details of auto-zeroing (100%T calibration). 3.6.3
Starting measurement measurement
After finishing parameter setting and Abs auto-zeroing, press [START/STOP] key to start measurement. Each time [START/STOP] is pressed, a measured value is obtained and calculation of the measured value multiplied by K-Factor is done. Both the measured value and the calculated value are saved in the control unit and displayed sequentially in the table on the LCD. When each measurement is finished, the sample number will increase by 1. The memory of the control unit can store up to 500 groups of measured data. The measuring process and the measured results may vary with the different settings in Section 3.5: (1) When Reagent Blank Correction in Cell No. 1 is set to “NO”, the system will start measurement to the set N samples (refer to§3.5.2). Then the measured value will be calculated, saved and displayed on LCD one by one. (2) When Reagent Blank Correction in Cell No. 1 (§3.5.3) is set to “YES”, the system will first measure the set N samples (refer to §3.5.2). Then the measured values of samples No.2~No.N will be deducted (corrected) by that of the blank in sample cell No. 1 following the formula below. The corrected results will be recorded, calculated and displayed on LCD one by one. Formula of deduction (correction): The absorbance of sample No.n = measured value of sample No.n – measured value of sample No.1(blank) The transmittance of sample No.n = measured value of sample No.n / measured value of sample No.1(blank) × 100%
3.6.4
Looking up the measured results
During measurement, users can look up the measured results line by line, by pressing the cursor keys [▲],[▼] . 3.6.5
Clearing off the measured data
During measurement, users can clear off the measured data at any time when [F2] key is pressed.
Fig. (3-10) Data deleting page in photometric measurement 3.6.6
Printing out
During measurement, users can print out the measured results in the memory at any time when [F4] key is pressed.
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Fig. (3-11) Data printing page in photometric measurement measurement 3.6.7
Quitting measuring operation
After measurement, press [RETURN] key to quit measuring operation. The system indicates that the data will be lost. Users can press [RETURN] key to return to the main menu of photometric measurement.
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Instruction manual
Spectrum Scanning This mode provides a wavelength scan and print out for absorbance, transmittance and energy of the measured sample.
4.1 Information Display and Function Keys On the instrument main menu (mode menu), press [2] key to select spectrum scanning mode. The main menu of spectrum scanning mode is displayed on LCD, as shown in Fig. (4-1).
Fig.(4-1) The main menu of spectrum spectrum scanning The first line on the menu indicates that the current working mode is spectrum scanning. In the upper part of operating area displays the current working wavelength (660.0nm) and the measured value (0.000Abs), while in the lower part is a coordinate diagram. On the right, the function of each function key is displayed. [F1] – parameter setting [F2] – peak-picking [F3] – sample control [F4] – spectrum printing On this page, wavelength setting, baseline correction and spectrum scanning can be done by depressing [GOλ] key, [AUTOZERO] key and [START/STOP] [START/STOP] key respectively.
4.2 Parameter Setting On the main menu of spectrum scanning, press [F1] key to display the parameter setting page, as shown in Fig. (4-2).
Fig. (4-2) Parameter setting page of spectrum scanning
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On this page, users can set the photometric mode, scanning speed, scanning wavelength range, ordinate full scale range and sampling interval by pressing the corresponding numeric keys. After parameter setting is finished, press [RETURN] key to return to the preceding page. 4.2.1 Setting photometric mode Press [1] key to set photometric mode. Four modes are available: Abs, Er, Es and %T , which can be selected in turn when [1] key is pressed. Thereinto Es and Er are the energy measuring on the reference beam and the sample beam respectively. When measuring the energy of the light beam, the energy gain will be asked to input first , and then the system executes a spectrum scan. 4.2.2 Setting scanning speed Press [2] key to set the scanning speed to Fast, Middle or Slow in turn. 4.2.3 Setting sampling interval Press [3] key to set sampling interval. You have 5 choices: 1nm, 2nm, 0.1nm, 0.2nm or 0.5nm, which can be selected in turn when [3] key is pressed. A maximum of 1100 points can be stored for one spectrum, because the memory space of microprocessor is limited. If the set wavelength range is wider than 110nm and the sampling interval is set to 0.1nm, the system will change it to 0.2nm automatically. In other circumstances, the setting will not be changed. 4.2.4 Setting wavelength range Press [4] key to display the wavelength range setting page of spectrum scanning. The system indicates user to set wavelength values, as shown in Fig. (4-3).
Fig. (4-3) Wavelength range setting page of spectrum scanning At the bottom of the page, first set the starting wavelength (long wave) by pressing numeric keys (0~9). [CE] key is used to clear off the preceding entry. After setting the starting wavelength, the system indicates user to set the ending wavelength (short wavelength). Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry. If the ending wavelength value entered is larger than that of the starting wavelength, an error message will be displayed. You should enter it again.
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4.2.5 Setting ordinate full scale range Press [5] key to display the ordinate (measured value) full scale range setting page shown as Fig. (4-4).
Fig. (4-4) Ordinate scale range range setting page At the bottom of the page, the system indicates user to set the upper limit of the ordinate. Press numeric keys (0~9), (.) and (-) to enter the desired value. [CE] key is used to clear off the preceding entry. After setting the upper limit, the system indicates user to set the lower limit. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from –9999~9999. If the figure entered exceeds four digits, the system will delete what has been entered. You should enter it again. 4.2.6 Dark Current Adjustment Press [F5 ] key to carry out dark current adjustment. The dark current adjustment is to be done, as shown in Fig. (4-5).
Fig. (4-5) Dark current current calibration page 4.2.7
Quitting parameter settings
Press [RETURN] key to quit parameter setting operation and return to the main menu of spectrum scanning.
4.3 Setting Measuring Wavelength On the main menu of spectrum scanning, press [GOλ] key to display the following page (Fig. 4-6) and set the measuring wavelength for spectrum scanning.
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Fig. (4-6) Wavelength Wavelength setting page page for spectrum spectrum scanning At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry.
4.4 Baseline Correction On the main menu of spectrum scanning, press [AUTOZERO] key to start baseline correction at the set wavelength range. Before starting baseline correction, put the blank sample into the sample compartment. [START/STOP] key can be pressed to stop baseline correction operation. If the wavelength range has been changed, the baseline calibration should be done again.
4.5 Measuring Operation On the main menu of spectrum scanning, press [START/STOP] key to start spectrum scanning measurement. The measured spectrum will be displayed dynamically, as shown in Figure 4-7. When the scan is to be stopped, press [START/STOP] [START/STOP] key again.
Fig. (4-7) (4-7) Spectrum scan operatin A maximum of 1100 points can be stored for one spectrum, because the memory space of micro-processor is limited,. If the set wavelength range is wider than 110nm and the sampling interval is set to 0.1nm, the system will change it to 1nm automatically. In other circumstances, the setting will not be changed.
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4.6 Peak-picking On the main menu of spectrum scanning, press [F2] key to display peak-picking operating page as shown in Fig. (4-8).
Fig. (4-8) Peak-picking operating page At the bottom of this page, enter the minimum height value of picked peaks/valleys by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. If the figure entered exceeds four digits, the system will delete what has been entered. You should start a new entry. After the minimum peak/valley value is entered, the system will start peak-picking calculation and the calculated result will be displayed on the screen. The peaks and valleys on the spectrum are marked. At the same time, an arrow indicates the current peak/valley point. Below the spectrum, the wavelength and the measured value of the pointed peak/valley are displayed. By pressing the [
]or [
] key, the arrow will be shifted to look up other
peak/valley points. Press [F4] key to print out the result. Press [RETURN] key to return to the main menu of the spectrum scanning after all the operations are ended.
4.7 Printing Out the Measured Result When spectrum scanning is finished, press [F4] key to print out the measured spectrum. [F4] key will not be effective if there is no spectrum stored.
Figure 4-9
spectrum printing page
Press [ENTER] key to print the spectrum or press [CE] key to quit printing.
4.8 Quitting Spectrum Scanning Measurement When spectrum scanning measurement operation is finished, press [RETURN] key to quit spectrum scanning. The system indicates that the data will be lost. Users can press [ENTER] key to return to the main menu of the instrument. 12
Instruction manual
Quantitative Measurement In this mode one-wavelength determination can be done. Both K-Factor Method and Calibration Curve Method are used in quantitative measurement.
5.1 Information Display and Function Keys On the instrument main menu, press [3] key to select the quantitative measurement mode. The main menu of quantitative measurement will be displayed as shown in Fig. (5-1).
Fig. (5-1) The main menu of quantitative measurement The first line on the menu is the mode line, which shows that the current operation mode is quantitative measurement. The upper part of the operating area shows the current measuring wavelength (500.0nm) and the measured value (0.000Abs), the lower part is a table in which the measured data are displayed. The right part of the page is the function key indicating area, which shows the functions of function keys [F1] ~[F4]. [F1] – parameters setting [F2] – clearing off the measurement data [F3] – sample control [F4] – printing On this page, the measuring wavelength setting, autozeroing and sample measurement can be carried out by depressing [GOλ] key, [AUTOZERO] key and [START/STOP] [START/STOP] key respectively.
5.2 Parameter Setting Parameter setting page (as shown in Fig. (5-2)) can be displayed by pressing [F1] key on the main menu of quantitative measurement.
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Fig. (5-2) Parameter setting page of quantitative measurement On this page, the measurement method, measuring wavelength and concentration unit can be set directly by pressing the numeric keys [1] ~ [3]. By pressing [F1] key the second time the calibration curve setting page can be displayed. After finishing all parameter settings, press [RETURN] key to return to the preceding page. 5.2.1 Setting measuremen measurementt method Press [1] key to set measurement method for quantitative measurement. There are two selections: K-Factor method and Calibration Curve method. The two methods will be selected alternately when [1] key is pressed each time. 5.2.2 Setting measuring measuring wavelength wavelength Press [2] key to display the wavelength setting page of quantitative measurement. The system will indicate users to enter the desired wavelength value, as shown in Fig. (5-3).
Fig. (5-3) Wavelength setting page of quantitative measurement measurement At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry. 5.2.3 Setting concentration concentration unit Press [3] key to display concentration unit setting page as shown in Fig.(5-4). The system will indicate users to select concentration unit. There are 8 choices: %, mg/l, ug/l, g/l, mg/ml, ng/ml, mol/l and no. The 8 units will be selected alternately when [3] key is pressed each time.
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5.2.4 Setting calibration curve curve parameters parameters (K-Factor method) method) Press [F1] key on parameter setting page of quantitative measurement to display the calibration curve parameter setting page. The content displayed on the page will vary with the method selected previously in Section 5.2.1. When K-Factor method is selected, the page shown in Fig. (5-4) will be displayed.
Fig. (5-4) Calibration curve parameter setting page for K-Factor method Press [1] key and [2] key to enter slope and intercept values of the calibration curve on this page. Press [F4] key to print out the parameters currently displayed on the screen. After all parameter settings are completed, press [RETURN] key to return to the preceding page. 5.2.4.1
Setting the slope of calibration curve
Press [1] key to display the slope setting page, as shown in Fig. (5-5).
Fig. (5-5) Slope setting page At the bottom of this page, enter the slope value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from 0 to 9999. If the figure entered exceeds four digits, the system will delete what has been entered. You should start a new entry. When a decimal number is entered, a “0” should be put before the decimal point, such as 0.12. 5.2.4.2
Setting the intercept of calibration curve
Press [2] key to display the intercept setting page, as shown in Fig. (5-6).
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Fig. 5-6 Intercept setting page At the bottom of this page, enter the intercept value by pressing the numeric keys (0~9), [.] and [-]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from 0 to 9999. If the figure entered exceeds four digits, the system will delete what has been entered. You should start a new entry. 5.2.4.3
Quitting calibration curve parameter setting
Press [RETURN] key to quit calibration curve parameter setting and return to the parameter setting page of quantitative measurement. 5.2.5 Setting calibration curve parameters parameters for Calibration Curve Curve method On parameter setting page of quantitative measurement, press [F1] key to enter the calibration curve parameter setting page. The content displayed on the page will vary with the method selected previously in Section 5.2.1. When Calibration Curve method is selected, the page shown in Fig. (5-7) will be displayed.
Fig.(5-7) Calibration curve parameter setting page for calibration curve method Press numeric keys [1]~ [3] to enter the number of standard samples, the concentration and absorbance values (if they are known) of the standard, and to carry out absorbance measurement to the standard. After finishing parameter setting, press [F3] key to set up calibration curve. After all parameter settings are completed, press [RETURN] key to return to the preceding page.
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5.2.5.1
Instruction manual
Setting the number of standards
Press [1] key to display standard number setting page as shown in Fig. (5-8), at the bottom of which a message is displayed to indicate users to enter the number of standards.
Fig. (5-8) Standard number setting page At the bottom of this page, enter the number of standards by pressing the numeric keys [1]~[8]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. A 1-digit figure entry is allowed. The range is from 1 to 8. If the figure entered exceeds 1 digit, the system will delete what has been entered. You should s tart a new entry. 5.2.5.2
Entering concentrations and absorbance of standards
Press [2] key to display concentration and absorbance setting page. The system will indicate users to enter the concentration and absorbance values of standard No. First, the system indicates users to enter the concentration, as shown in Fig. (5-9).
Fig. (5-9) Concentration and absorbance absorbance setting page At the bottom of this page, enter the concentration value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. After the concentration is entered, the system will indicate users to enter the absorbance values. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from 0 to 9999. If the figure entered exceeds four digits, the system will delete what has been entered. You should start a new entry. When a decimal number is entered, a “0” should be put before the decimal point, such as 0.5. Press [2] again and repeat the above procedure if concentration and absorbance of other standards are to be entered.
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5.2.5.3
Instruction manual
Starting standard sample measurement measurement
Press [3] key to display the standard sample measurement page as shown in Fig. (5-10). The system indicates that the sample to be measured should be placed in Cell No.1.
Fig. (5-10) Standard sample measurement measurement page After the standard sample is placed well in the cell, press [START/STOP] key to start measurement. The measured result will be displayed on LCD. Users can repeat measurement to the same sample for several times, but only the last measured result is saved. After the measurement is finished, press [RETURN] key to quit. Press [3] again and repeat the above procedure if the absorbances of other standards are to be measured. 5.2.5.4
Building the calibration curve
After the data of standard samples are entered and measurement is completed, press [4] key to carry out curve fitting and set up the calibration curve. Parameters of the calibration curve will be displayed on LCD as shown in Fig. (5-11).
Fig. (5-11) Calibration curve set-up page Press [RETURN] to return to calibration curve parameter setting page. 5.2.5.5
Printing out the calibration curve
Press [F4] to print out parameters of the calibration curve in the format displayed on LCD. The system indicates if the printer is OK or not. If the printer is OK, press [ENTER] key to print it out.
5.2.5.6
Quitting calibration curve parameter setting
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Press [RETURN] key to quit calibration curve parameter setting, and return to parameter setting page in quantitative measurement. 5.2.6
Quitting parameter setting of quantitative measurement measurement
Press [RETURN] key to quit parameter setting of quantitative measurement and return to the main menu of quantitative measurement.
5.3 Auto Zeroing On the main menu of quantitative measurement, press [AUTOZERO] key to set Abs zero automatically at current measuring wavelength. Before starting auto zeroing, put the blank sample into the cell.
5.4 Setting Measuring Wavelength Press [GOλ] key to set the measuring wavelength on the main menu of quantitative measurement, which is shown in Fig. (5-12).
Fig. (5-12) Wavelength setting page in quantitative measurement At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit this page directly. A 4-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds four digits, the system will delete what has been entered automatically. You should start a new entry.
5.5 Sample Control On the main menu of photometric measurement, press [F3] key to display the sample control page as shown in Fig. (5-13).
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Fig. (5-13) Sample control page in photometric measurement measurement Users can select sample cell-holder type (fixed cell-holder, 5-cell holder and 8-cell holder) in the sample control page. If the 8-cell holder is selected, you can set the working parameters of the 8-cell holder by pressing the corresponding numeric keys. After finishing the parameter setting, press [RETURN] key to return to the preceding page. 5.5.1 Selecting sample module type Press [1] key to select sample module type. There are three types available, fixed cell-holder, 5-cell holder and 8-cell holder, which can be selected alternately each time [1] key is pressed. 5.5.2 Setting sample cell number in use (Drive Cell No.) This parameter is only available to 5-cell holder and 8-cell holder. Press [2] key to enter the setting page (as shown in Fig.5-14). The system will indicate you to enter the number of sample cell in use (Drive Cell No.). At the bottom of this page, enter the sample cell number by pressing the numeric keys (0~9). [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. A 1-digit figure entry is allowed. The range is from 1 to 8. If the entered figure exceeds the range, the system will delete what has been entered. You should start a new entry. In the process of measurement, the system will measure the samples in the set sample cells one by one. The serial numbers of the sample cells are expressed by n-1, n-2,….
Fig. (5-14)
Sample cell number setting page
5.5.3 No. 1 Cell blank correction correction This parameter is used to set No. 1 cell blank calibration function which means to calibrate the measuring result of the sample in each sample cell (No. 2-8) with that of the blank in the No. 1 cell. In measurement, the system will deduct the measured value of the blank in No. 1 cell from that of the samples in the same cell (No.2 ~ No.8), the 8
Instruction manual
result of which will be the measuring result. Two choices are available for this item: YES and NO, which alternate with each other each time [3] key is pressed.
5.5.4 Sample cell shifting (Move Cell) Press [4] key to shift the cell holder to the next sample cell.
5.5.5 Resetting to cell No.1(Move Home) Press [5] key to reset the cell holder to sample cell No.1.
5.5.6 Single-cell measurement If the single-cell type is selected, you can choose any of the cells of the multi-cell as the measuring cell by shifting the cell holder to the desired position according to the method described in 3.5.6. Measurement will only be done to the chosen cell.
5.5.7 Quitting sample control When sample control operation is finished, users can press [RETURN] key to quit sample control function and return to the main menu of photometric measurement.
5.6 Measuring Operation After all measuring parameters are set and blank corrections are made, press [START/STOP] key to start measurement. Each time [START/STOP] key is pressed, a measured Abs value is obtained and the concentration of the measured sample will be calculated. Both the measured Abs value and the calculated concentration value will be saved in the control unit and displayed sequentially in the table on the LCD. The sample number will increase by 1 after each measurement. The memory of the control unit can store up to 500 groups of measured data. The measuring process and the measured results may vary with the different settings in Section 5.5: (1) When Reagent Blank Correction in Cell No. 1 is set to “NO”, the system will start measurement to the set N samples (refer to§5.5.2). Then the measured value will be calculated, saved and displayed on LCD one by one. (2) When Reagent Blank Correction in Cell No. 1 (§5.5.3) is set to “YES”, the system will first measure the set N samples (refer to §5.5.2). Then the measured values of samples No.2~No.N will be deducted (corrected) by that of the blank in sample cell No. 1 following the formula below. The corrected results will be recorded, calculated and displayed on LCD one by one. Formula of deduction (correction): The absorbance of sample No.n = measured value of sample No.n – measured value of sample No.1 (blank) The transmittance of sample No.n = measured value of sample No.n / measured value of sample No.1 (blank) × 100%
5.7 Looking Up the Measured Results During measurement, users can look up the measured results line by line, by pressing the cursor keys [▲],[▼] .
5.8 Printing Out During measurement, users can print out the measured results in the memory at any time when [F4] key is pressed.
5.9 Quitting Quantitative Measurement After finishing quantitative measurement, press [RETURN] key to quit quantitative measurement operation. When [RETURN] key is pressed, the system will indicate that the measured data will be cleared off. Users can press [ENTER] key to return to the main menu of the instrument operation.
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Instruction manual
DNA/Protein Analysis In this mode DNA/Protein analysis can be done. There are three methods: Method 1, Method 2 and Self-defining.
6.1 Information Display and Function Keys On the instrument main menu, press [4] key to select the DNA/Protein mode. The main menu of quantitative measurement will be displayed as shown in Fig. (6-1).
Figure 6-1 The main menu of DNA/Protein Analysis The first line on the menu is the mode line, which shows that the current operation mode is DNA/Protein analysis. The upper part of the operating area shows the current measuring method and the corresponding wavelengths (500.0nm and 230.0nm) and the lower part is a table in which the measured data are displayed. The right part of the page is the function key indicating area, which shows the functions of function keys [F1] ~[F4]. [F1] – parameters setting [F2] – clearing off the measurement data [F4] – printing On this page, the autozeroing and sample measurement can be carried out by depressing [AUTOZERO] key and [START/STOP] [START/STOP] k ey respectively. re spectively. Press [
]
[
] to change A1, A2, DNA concentration, protein concentration alternately, as shown in Figure 6-2.
Figure 6-2 Concentration display page of DNA/Protein
6.2 Setting Parameters Parameter setting page (as shown in Fig. (6-3)) can be displayed by pressing [F1] key on the main menu of
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DNA/Protein analysis.
Figure 6-3 Parameter setting page of DNA/Protein analysis Press [
]
[
] to select the measuring methods. Method 1 and Method 2 are the system defaulted parameters
that can not be changed by users. Users can set parameters according to their requirements under the self-defining mode. After finishing all parameter settings, press [RETURN] key to return to the preceding page. 6.2.1 Setting DNA/Protein analysis method Press
[
]
[
] to select DNA/Protein measuring methods. The selected method will be reverse displayed.
6.2.2 Setting measuring wavelength Under the custom mode, press [1] key to display the wavelength setting page of DNA/Protein analysis. The system will indicate users to enter the desired wavelength value, as shown in Fig. (6-4).
Figure 6-4 Wavelength setting page of DNA/Protein analysis At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. The system will indicate users to input the second wavelength after inputting the first one. Press [ENTER] key to confirm the numeric entry. If nothing is to be entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry. 6.2.3 Setting DNA factor Under the self-defining mode, press [2] key to display the DNA factor setting page. The system will indicate users to
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input DNA factor, as shown in Figure 6-5.
Figure 6-5 DNA/Protein factor setting page At the bottom of this page, enter the desired protein factor by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. The system will indicate users to input the second factor after inputting the first one. Press [ENTER] key to confirm the numeric entry. If nothing is to be entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from –9999 to 9999. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry. 6.2.4 Setting protein factor Under the self-defining mode, press [3] key to display the protein factor setting page. The system will indicate users to input Protein factor, as shown in Figure 6-6.
Figure 6-6 Protein factor setting page At the bottom of this page, enter the desired protein factor by pressing the numeric keys (0~9), [.]. [CE] key is used to clear off the preceding entry. The system will indicate users to input the second factor after inputting the first one. Press [ENTER] key to confirm the numeric entry. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from –9999 to 9999. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry. 6.2.5 Setting background calibration at 320nm Under the self-defining mode, press [4] key to set background calibration at 320nm. Two choices are available for this item: YES and NO, which can be selected alternately each time [4] key is pressed. 6.2.6 Setting calibration factor Under the self-defining mode, press [5] key to display the calibration factor setting page. The system indicate users to 3
Instruction manual
input the calibration factor, as shown in Figure 6-7.
Figure 6-7 Intercept setting page At the bottom of this page, enter the desired calibration factor by pressing the numeric keys (1~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from –9999 to 9999. If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry.
6.3 Quitting Parameter Setting of DNA/Protein Press [RETURN] key to quit DNA/Protein parameter setting and return to the main menu of DNA/Protein.
6.4 Autozeroing On the main menu of DNA/Protein, press [AUTOZERO] key to set Abs zero automatically at current measuring wavelengths. Before starting auto zeroing, put the blank sample into the cell.
6.5 Measuring Operation On the main menu of DNA/Protein, press [START/STOP] key to start measurement. Each time [START/STOP] key is pressed, a measured Abs value is obtained. The measured Abs value will be saved in the control unit and displayed sequentially in the table on the LCD. The sample No. will increase by 1 after each measurement. The memory of the control unit can store up to 100 groups of measured data.
6.6 Looking Up the Measured Results During measurement, users can look up the measured results line by line, by pressing the cursor keys [▲ ],[ ▼] .
6.7 Printing Out After measurement, users can print out the measured results in the memory at any time when [F4] key is pressed.
6.8 Quitting Measurement Measurement After finishing measurement, press [RETURN] key to quit measurement. The system will indicate that the measured data will be lost. Press [ENTER] key to return to the instrument main menu.
Utilities In this mode, the operating parameters can be set, such as lamp changeover wavelength, spectral bandwidth, tungsten
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lamp On/Off, deuterium lamp On/Off and wavelength calibration etc..
7.1 Information Display and Function Keys On the instrument main menu, press [5] key to select the Utilities mode. The main menu of utilities will be displayed on LCD, as shown in Fig. (7-1).
Fig. (7-1) (7-1) Main menu menu of utilities utilities The first line on the page is the mode line, which shows the current operating mode, the Utilities mode. In the right part of the page , the function indicating area, there is no indication for function keys [F1] ~[F4]. On this page, [GOλ], [AUTOZERO] and [START/STOP] keys are not to be used.
7.2 Setting Lamp Changeover Wavelength On the main menu of utilities, press [1] key to set lamp changeover wavelength, the page of which is shown in Fig. (7-2).
Fig. (7-2) Lamp changeover WL setting page At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9). [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press [RETURN] key to quit this page directly. A 4-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds four digits, the system will delete what has been entered automatically. You should start a new entry.
7.3 Setting Spectral Bandwidth Press [2] key to set the spectral bandwidth of the instrument. Four options are available: 2.0, 5.0, 0.5, 1.0nm. They can be selected in turn when [2] key is pressed each time.
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SPECIAL NOTE: Every time you change the bandwidth shouls do the dark current correction, wavelength correction and insert the blank to do the baseline.
7.4 Setting W Lamp On/Off Press [3] key to display tungsten lamp on/off setting page. On and Off can be changed in turn when [3] key is pressed each time.
7.5 Setting D2 Lamp On/Off Press [4] key to display deuterium lamp on/off setting page. On and Off can be changed in turn when [4] key is pressed each time.
7.6 Setting Control mode Press [5] key to change control mode between PC mode and MCU mode, then hit Return in the Keypad to go back to the main menu.
7.7 Setting time Press [6] key to display time setting page. Press [▲] and [▼] to set time. Press [ day, month and year, as shown in Fig. (7-3).
Fig. (7-3) Time setting page
6
][
] to set minute, hour,
7.8 Dark Current Adjustment Press [7 ] key to carry out dark current adjustment, as shown in Fig. (7-4).
Fig. (7-4) Dark current current adjustment page page
7.8
Wavelength Calibration Calibrat ion (Wavelength Adj)
Press [8] key to carry out wavelength calibration function, as shown in Fig. (7-5).
Fig. (7-5) Wavelength Wavelength calibration calibration page
7.9
Quitting Utilities Mode
Press [RETURN] key to quit utilities mode and return to the instrument main menu
7
Instruction manual
Maintenance Spectro Dual Split Beam UV/VIS Spectrophotometer is a precision instrument. Careful assembling and final test have been done before delivery. Appropriate routine attention and maintenance will not only ensure the reliability and stability of the instrument but also extend its operating lifetime.
8.1 Points for Attention
A good environmental condition should be provided according to the requirements in Section 1.4.
Since the instrument has been adjusted to its optimum conditions before delivery, users are neither allowed to adjust it by themselves nor dismount parts from the instrument. Pay special attention not to touch and damage the surfaces of optics or to wipe the surfaces carelessly.
8.2 Daily Care
Check if there is spilt sample solution contained in the sample compartment after each measurement. The sample compartment should be cleaned frequently to avoid corrosions of the parts and optical the system.
The instrument should be covered with anti-dust cover, which is provided with the instrument. Silica-gel bags can be put in the sample compartment and the light source housing to keep dry. But be sure to take the bags out before switching on the instrument.
During daily operation and storage, the LCD display and keyboard should be handled with care and prevented from scratch, water, dust and corrosion.
Periodically check the performance of the instrument. If anything abnormal occurs, contact the seller or the manufacturer immediately.
If the instrument is not to be used for a long time, pay great attention to environmental conditions such as temperature and humidity and periodically replace the silica-gel bags.
8.3 Trouble Shooting and Maintenance 8.3.1 Diagnoses of initialization initialization When the power switch is turned on, the spectrophotometer makes various checks and sets many initialized parameter values (as shown in Fig. (8-1)). The system gives status information after each operation is completed. If no abnormality is detected, it displays “OK”. If an abnormality is detected, it displays “Err” and asks whether the initialization continues or not. The status of instrument will be known after finishing the initialization.
Fig.(8-1) Initialization page 8.3.2 Trouble shooting If the spectrophotometer malfunctions, it probably is due to the deterioration of consumable parts, operator mis-operation or incomplete maintenance. Make checks according to the following table. If a major difficulty occurs, contact our service personnel. Table 8-1
Phenomena The instrument does not operate at all, after the power is turned on.
Causes A) Power cable is not securely connected; B) Fuse is blown; C) Circuit failure. A) Printer failure; B) CPU failure.
Spectrum can not be printed out. Initialization abnormality: 1) ROM check A) ROM failure; 2)Wavelength 2)Wavelength origin; 3)
Multi-cell origin;
4)
Slit origin;
5)
Filter origin;
6)
W lamp energy;
7)
D2 lamp energy;
8) Wavelength check
Noise abnormality
A) Abnormality of WL origin ; A) Abnormality of multi-cell holder drive origin; A) Slit mechanism failure A) Filter mechanism failure; A) Opaque object in the sample compartment; B) W lamp is not lit A) Opaque object in the sample compartment; B) D2 lamp is not lit;
A) Opaque object in the sample compartment; B) Wrong positioning of D2 lamp. A) Light source ageing; B) Wrong position of the sample compartment; C) Low voltage / strong magnetic field; D) Receiver ageing ; E) Preamplifier board failure; F) Abnormality of AD converter.
Diagnoses A) Check the power cable; B) Check the fuse; C)Check C) Check the instrument. A) Check printer; B)Check B) Check CPU board. A) Switch on power again to check; A) Switch on power again to check; A) Shift cell holder to check; B) Loose mechanical connection; A) Switch on power again to check; A) Switch on power again to check; A) Open the sample compartment; B) Open the light source housing; A) Open the sample compartment; B) Open the light source housing; C) Remove the cover of instrument; A) Open the sample compartment; B) Open the light source housing. A) Measure in Energy mode; B) Observe at 550nm; C) Check voltage/source of magnetic field; D) Measure in Energy mode; E) Check the preamplifier board; F) Check the drive board.
Remedy A) Connect it securely; B) Replace the fuse (2A); C) Contact our service personnel. Contact our service personnel. A) Contact our service personnel; A) Contact our service personnel; A) Remove the obstacle; B) Tighten the connecting mechanism; A) Contact our service personnel; A) Contact our service personnel; A) Remove the opaque object; B) Replace the W lamp ; A) Remove the opaque object; B) Replace the D2 lamp; C) Replace the fuse of D2 lamp; A) Remove the opaque object; B) Reposition the D2 lamp. A) Replace the light source; B) Reposition the sample compartment; C) Add voltage stabilizer /eliminate interference; D) Contact our service personnel; E) Contact our service personnel; F) Contact our service personnel.
8.4 Light Source Replacement 8.4.1 Replacement of the W lamp 1.
The tungsten lamp is plugged in a socket and sleeved by a plastic holder. It is well adjusted before delivery. Directly insert a new one when lamp replacement is made.
2.
Open the light source housing cover. Loosen the two holding screws (1) on the W lamp socket and remove the W lamp holder (W) (refers to Fig. 8-2).
3.
Replace a new W lamp holder. Clean the surface of the W lamp with a clean cloth socked with alcohol.
4.
Insert the W lamp holder into the W lamp socket in the light source housing and tighten the two holding screws (1).
5.
If the performance of the instrument changes greatly after the replacement, the height of W lamp should be
adjusted (The horizontal position of W lamp is adjusted automatically when the self-testing is done upon switching on the power.). The adjusting procedure is as follows: a)
Remove the W lamp hold er.
b)
Loosen the three screws (2) on the W lamp holder with a watch screwdriver.
c)
Adjust the height of W lamp.
d)
Tighten the three screws (2) .
e)
Insert the W lamp holder back to the socket.
8.4.2 Replacement of the D2 lamp 1.
The D2 lamp is plugged in the D2 lamp socket. When replacement is made, directly and securely insert a new one into the socket.
2.
Open the light source housing cover. Remove the D2 lamp (D) (refers to Fig. 7-2) .
3.
Replace a new D2 lamp. Clean the surface of D2 lamp with a clean cloth socked with alcohol.
4.
Insert the D2 lamp into its socket. [Note: The light beam window should face to the reflective mirror (G)]
PRECAUTIONS: The light source and light source housing are considerably heated during operation. Therefore, be sure to turn off the power switch so as to cool down the light source sufficiently before replacing the light source. The reflective mirror (G) should be covered with paper bag to avoid being contaminated by fingers.
Fig. (8-2) Diagram of light source housing
CHANGING THE NUMBER OF CELLS IN SPECTRO UVS – 2700/2800 UVD – 2950/3000/3200
Fig. 1 To change the number of Cells, first open UVWin5 Config Program located in Start, All Programs as shown on Fig. 1.
Fig. 2 UVWin Configuration window will open; Select the tab (Accessories). Fig. 2 Shows that Fixed cells is the Default configuration.
Fig. 3
Fig. 3 Shows Multy-cells of 8 has been selected and will be the new configuration. Once the new settings are configured simply apply the settings to your software by pressing theOK the OK button.
INITIALIZATION INITIALIZATION INSTRUCTIONS Troubleshooting for the lamp self-check error of Spectro UVS-2700/2800, UVD-3000/3200, UVD-3400/3500
Trouble phenomenon p henomenon When the initialization carries out to “W lamp check”, the message with system error comes out. Solution Please check the trouble cause step by step according to the following step. 1. Check if there if there is Opaque object which interdicts the light path in the sample compartment. 2. Check if the W lamp and the D2 lamp is lit. 3. Check if the correct cell holder (fixed cell-holder, 5-cell holder and 8-cell holder) is selected. 4. Check if the alignment of light source source goes wrong, according according the following file “How to adjust alignment of light source of Spectros”. 5. Check if the boards go wrong according the following file “How to adjust the amplifier boards” Special notice: 1. Serviceman can press “F5”key to burst into the main menu and operate the instrument, when the initialization fault or The instrument information appear. 2. The operation is only for service men. The instrument is in abnormal mode now, many parameters are not right, and can’t check specifications. The user should avoid bursting into the operation menu to make the instrument work abnormally.
How to Adjust alignment of light source of Spectros S pectros 1
Condition of adjusting the light source mirror: The light source mirror has been well adjusted before ex-work. Customers need not to do any alignment unless the following cases: After replacement of the light source mirror, alignment of initial positioning point of the light source mirror is needed. If the light source mirror is loosed due to, for example, transportation, alignment of initial positioning point of the light source mirror is needed. If the message of light source initialization error occurs during instrument initialization, please do not adjust the position of the light source mirror inconsiderately. Please check carefully the whole process of light source initialization to judge if alignment of initial positioning point of the light source mirror is needed. 2 The process of light source initialization When instrument is powered on and initialization program is carried out to light source initialization, please check carefully the following moving actions: The light source mirror should turn round left at first. After finding photo switch, the W lamp is lit and D2 lamp preheats.During preheats.During preheating, the D2 lamp doesn’t be lit and the light spot of W lamp is focused on the positon of about 3mm left to the entrance slit(Position 1 in the following figure). After preheating, the D2 lamp is lit and the light source mirror turns round right slowly to make the light spot of W lamp goes across the entrance slit. After the light spot of W lamp goes through the slit, the light source mirror starts to turn round right fleetly to make the light spot of D2 lamp be focused on the position of about 3mm left to the entrance slit (Position 1 in the following figure). Then the light source mirror turns round right slowly to make the light spot of D2 lamp go across the entrance slit After the light slit goes across the entrance slit,the light source mirror turns round left to locate the light spot of D2lamp on the entrance slit. If the real moving actions are the same as upper descriptions, and instrument gives the error message of low lamp energy, please check if other part of sample signal receiving is ok or not. 3 Adjusting the initialization position of light source mirror Turn on the power of instrument. When initialization program is carried out to light source initialization, and the light of D2 lamp and W lamp are not focused on Position 1 in the following figure, please do as the followings:
Open the cover of the instrument. Unscrew the screws one round on position 2 in the following picture. Turn on the instrument and it Performs initlalization action.
When the W lamp are lit,D2 lamp starts to preheat(about 10 seconds).During preheating, turn round the mirror manually to make the light of W lamp focused on the position of about 3mm left to the entrance slit.If the height of the light spot isn’t correct,please adjust the 2 screws in front of the light source mirror to fit the height of the entrance slit. After preheating,the D2 lamp is lit and the initialization action continues. After the initialization is done successfully, the light spot of D2 lamp should go to the entrance slit, At this time screw the mirror completely, completely, Re-turn on and start initialization to check the new light source initialization. After the lamps are lit, that is ok if the lights li ghts of W lamp and D2 lamp go across the slit in turn. Notice specially that the sample compartment should be Covered completely with opaque covering to avoid the outside light to enter the Sample compartment,when adjusting the positin of the light Source mirror. Or else the message “system error”will come out,when initlalization.
How to adjust the amplifier boards
The old version
the prime amplifier board
the preamplifier board Adjust the prime amplifier 1．Enter into the menu of photometric measurement after initialization (if the initialization fails, burst into it). 2．Pull out the plug of J204 of prime amplifier board, and short the pins 2-3 of the J204. 3．Press F1 to enter into the menu of parameters setting, set the photometric mode to Es, wavelength range to 700-600nm, range 0-100; sampling interval to 1nm. 4．Press RETURN to return to the measurement menu, then press START START, setting the gain to 8, and then begin to scan. 5．Adjust the potentiometer VR201. If the energy linearly varies with the potentiometer adjustment in step, the prime amplifier is all right.(Adjust the energy value to 5 finally)
6．If the energy value does not change or change widely when adjusting the potentiometer, the prime amplifier is not all right
The initialization procedures for Spectro UVS-2700/2800 – UVD-3000/3200 and UVD-3500 1. Turn on the instrument, it will show the following menu. a. The instrument is under the MCU mode.
b. The instrument is under the PC mode.
Logo menu
Initialization menu
In the case of PC mode, the instrument can be controlled by UVWin 5. If pressing START START key, key, It’s mode will be changed to MCU mode. 2. RAM Check The instrument check if the memory goes wrong or not. 3. Cell Motor Reset If 5 cell holder or 8 cell holder is used, the instrument will perform the cell motor reset procedure and make No.1 cell in the light path. The reset processes are as follows:
Position sensor
8 cell holder
5 cell holder
a.
The cell moves inwards inwards towards the position sensor (photo (photo electricity switch).
b.
Then the cell moves outwards outwards to locate the No.1 cell in the light path. path.
Note: a.
If you have replaced the cell holder with another another model, please press “F5” in the case case of logo menu to burst into the main menu and gain the access to photometric measure mode, press “F3” key into Sample control menu and select relevant sample module and turn off and on the main unit and perform the initialization procedure.
Main menu
Photometric mode b.
Photometric mode
Sample control menu
It’s one of all reasons that the instrument can’t work normally normally if the light doesn’t doesn’t go through the the center of the No.1 cell or the light is blocked completely. After successful initialization, change the current
wavelength to 546nm and use a piece of slip to check if the light goes through the center of the No.1 cell. Special notice: a.
Serviceman can can press “F5”key to burst into the main menu and operate operate the instrument, when when the initialization fault or the instrument information appear. appear.
b. The operation is only for service men. The instrument instrument is in abnormal mode now, now, many parameters are not right, and can’t check specifications. The user should avoid bursting into the operation menu to make the instrument work abnormally abnormally.. Troubleshooting
If the light doesn’t go through the center of the No.1 cell, Please adjust the fore-and-aft position of the position sensor to fit the light path. 4. WL Motor Reset
Scan mechanism picture 1- guide screw 2- slit position sensor (photo electricity switch) 3- sine bar 4- screw A 5- grating rotating shaft 6- scan position sensor (photo electricity switch) 7- scan motor 8- slit motor 9- slit locating slip The WL motor reset processes are as follows: a.
The sine bar moves slightly towards the short short wave direction.
b.
The sine bar bar moves towards towards the long wave wave direction to the position sensor, sensor, after after getting to the position sensor, sensor, moves towards the short s hort wave direction to somewhere.
Note: The position sensor is for the initial position, it’s absolutely necessary to check the initial position of the sine
bar before use, or else it maybe damage the scan mechanism badly. badly. Troubleshooting
If the WL Motor Reset err, please check the scan mechanism as follows: a. Check if the guide screw can turn swimmingly or not. If it is rusty or with a lot of dust, it can not turn swimmingly to make the sine bar get to the position sensor. sensor. b. Check if the position sensor goes wrong or not. If the position goes wrong, the sine bar will not shop moving at the position sensor until the limit.
5. Slit Motor Reset If the instrument is with alterable slit, this item is included in the initialization procedures. Refer to the Scan mechanism picture, the reset processes are as follows: a.
The slit locating slip turns around clockwise towards towards the slit position sensor. sensor.
b.
The silt locating slip turns turns around anticlockwise anticlockwise towards somewhere somewhere after reaching the slit position sensor.
Note: It’s necessary to check the initial position of the slit motor before use, or else it maybe damage the instrument badly. Troubleshooting
If the Slit Motor Reset err, please check as follows: a.
Check if the slit locating slip can can turn swimmingly to the slit position position sensor. sensor.
b.
If the slit position sensor sensor goes wrong, the the slit locating slip will not stop moving at the position position sensor until the limit.
6. Filter Motor Reset If only opening the instrument cover, the filter motor is invisible, because it is inside the optical system. The reset processes are as follows: a.
The filter locating slip turns around anticlockwise towards towards the slit slit position sensor. sensor.
b.
The filter locating slip turns around clockwise towards somewhere somewhere after reaching the filter position sensor.
Note: Laypeople had better not open the optical system! If the filter motor reset fails, please contact the professional serviceman or manufacturer. manufacturer. 7. Lamp Motor Reset The reset process is as follows: The light source mirror turns around anticlockwise towards the position sensor (under the light source compartment) and stay at the position sensor.
Light source motor
Light source mirror locating slip
Position sensor
The bottom view of light li ght source compartment Note: a.
For avoiding scalding, please don’t touch the tungsten lamp and the the deuterium lamp. lamp.
b.
Don’t touch the light source source mirror.
Troubleshooting If Lamp Motor Reset err, err, Maybe the lamp source locating locating slip could not not get to the position sensor or
the
position sensor goes wrong. 8. W Lamp Check As soon as the light source mirror reaches the initial position, W lamp is lit and D2 lamp is preheated. At this moment, the tungsten lamp light spot should be focused on the position of about 3mm left to the entrance slit (Position 1 in the following figure).
3
3. Screw Light source picture
After preheating about 10 seconds, D2 lamp is lit. The light source mirror turns clockwise slowly and the W lamp light spot goes across the entrance slit. Note: a.
The centers of light spot and entrance slit should be on the same same water level. level.
b.
During W lamp Check, the sample sample compartment should be covered. If you open the cover cover of instrument instrument for observing the whole initialization process, please cover the sample compartment with a opaque object against sunlight. Or else W lamp Check will fail.
c.
It’s one of all reasons that the instrument can’t work normally normally or W lamp energy is low if the light spot doesn’t go through the center of the entrance slit or the light is blocked completely. completely. Chapter4 Troubleshooting
During deuterium lamp is preheating, if W lamp are not focused on Position 1 (about 3mm left to the entrance slit) in the Light source figure, please do as follows: a.
Turn off off the instrument, open the cover, cover, Unscrew the screws screws one round on position 2 in the following following picture.
b.
Turn on the instrument and perform self-check, During During preheating (about (about 10 seconds), seconds), turn round the mirror manually to make the light of W lamp focused on the position 1 (about 3mm left to the entrance slit).
c.
If the height of the light spot isn’t correct, please adjust the 2 screws on position position 3 to fit the height of the entrance slit.
d.
After the initialization is done successfully successfully,, the light spot of W lamp should go to the entrance slit, At this time screw the screws on position 2 completely, Re-turn on and start initialization and observe the position of the light spot.
9. D2 lamp Check After the W lamp light spot goes across the entrance slit and W lamp Check passes, the light source mirror turns anticlockwise fleetly to locate the D2 lamp light spot, then the light source mirror turns anticlockwise slowly to make the light spot of D2 lamp go across the entrance slit.
Note: a.
The centers of light spot and entrance entrance slit should be on the same water level.
b.
During D2 lamp Check, Check, the sample compartment compartment should be covered. covered. If you open open the cover of instrument instrument for observing the whole initialization process, please cover the sample compartment with a opaque object against sunlight. Or else D2 lamp Check will fail.
c.
It’s one of all reasons that the instrument can’t work normally or D2 lamp energy is low if the light light spot doesn’t go through the center of the entrance slit or the light is blocked completely.
Troubleshooting
If the position of W lamp light spot fits the position of the entrance slit well, the D2 lamp light spot should be correct. 10. Wavelength Check After D2 lamp Check passes, locating the D2 lamp light spot on the entrance slit and checking the wavelength accuracy at the point of 656.1nm. Note: The error message will appear, if can not find out the characteristic peak at the point of 656.1nm in the range between 636.1nm and 676.1nm. At this time, you can press F5 key to burst into main menu gain access to spectrum measurement, scan D2 lamp energy curve between 700nm and 400nm so that you can check the wavelength accuracy. Troubleshooting 1). If you find the wavelength of the whole instrument is inaccurate, you can perform the wavelength correction on the utilities menu. 2). If the performance of wavelength correction has no effect on it, please make the following adjustments: a. The light spot of the tungsten lamp should be at the incident slit after performing self-checking. Pull out J302 plug on the PC3 driving board. Manually rotate the coupling shaft to adjust the distance between A and B to 4mm. b. Put a piece of white tissue behind the filter. (Note: Don’t scratch the mirror face) Loosen screw A, and then rotate the grating rotating shaft slightly to make a zero order spectrum on the tissue. Tighten screw A. (Note: the zero order spectrum should still be on the tissue after fastening screw A.) Take out the tissue, put the cover on the reference optical circuit. Turn off the power supply and insert J302. Manually rotate the coupling shaft to adjust the distance between A and B to 30mm. Start the instrument and perform self-check again. c. After performing the self-check, pick the two characteristic peaks of the deuterium lamp. Read the wavelength of the two peaks. d. If the difference between the two peaks is more than 170.1nm, loosen the screw A and slightly rotate the grating rotating shaft counterclockwise, then fasten screw A. If the difference between the two peaks is less than 170.1nm, loosen the screw A and slightly rotate the grating rotating shaft clockwise, then fasten the screw A. Rescan the two peaks and recalculate the difference between the two peaks. You can’t stop adjusting until the difference is 170.1nm. (Note: it is possible that the two wavelengths for the two peaks are not standard wavelengths)
11. Parameter Init Initialize the system parameter.
1． Turn on the instrument, press F5 quickly, and enter into the main menu. 2． Select the direct current 20V by a multimeter.
3． Insert the black pen into the pin 2 of the J202 on the prime amplifier board. 4． Check the test point TP202 by a red pen. 5． Open the cover of the sample compartment, block the receiver, verify the voltage value, and then take off the opaque object to observe the change of the voltage of the multimeter. If the voltage changes obviously, the preamplifier is all right. 6． Check the reference amplifier by using the above method. Check the test point TP201 by the red pen. 7． Further confirmation: block the amplifier, adjust the potentiometer of the preamplifier board, and measure the voltage. If the voltage changes, the preamplifier is all right. Method 2: indirect measurement measurement 1． Verify the prime amplifier is all right. 2． Enter into the menu of photometric measurement after initialization (if the initialization fails, burst into it). 3． Press F1 to enter into the menu of parameters setting, set the photometric mode to Es, wavelength range to 700-600nm, range 0-100; sampling interval to 1nm. 4． Press RETURN to return to the measurement menu, then press START, setting the gain to 8, and then begin to scan. 5． Open the cover of the sample compartment, block the receiver, verify the energy value, and then take off the opaque object to observe the change of the energy value. If it changes obviously, the sample preamplifier is all right. Adjust the potentiometer of the sample preamplifier. Observe the changes of the energy. If the energy linearly changes with the adjustment of the potentiometer, the preamplifier is all right.
The new version
the prime amplifier board
the preamplifier board 8Adjust the prime amplifier 1.Enter into the menu of photometric measurement after initialization (if the initialization fails, burst into it). 2.Pull out the plug of J203 of prime amplifier board, and short the pins 2-3 of the J203. 3.Press F1 to enter into the menu of parameters setting, set the photometric mode to Es, wavelength range to 700-600nm, range 0-100; sampling interval to 1nm. 4.Press RETURN to return to the measurement menu, then press START, setting the gain to 8, and then begin to scan. 5.Adjust the potentiometer VR201. If the energy linearly varies with the potentiometer adjustment in step, the prime amplifier is all right.(Adjust the energy value to 5 finally) 6.If the energy value does not change or change widely when adjusting the potentiometer, the prime amplifier is not all right. 9Adjust the preamplifier Method 1: Direct measurement: 1.Turn on the instrument, press F5 quickly, and enter into the main menu. 2.Select the direct current 20V by a multimeter. 3.Insert the black pen into the test point TP203 (AGND) of the prime amplifier board or the pin 2 of J1019 of the power bus. 4.Check the test point TP205 by a red pen. 5.Open the cover of the sample compartment, block the receiver, verify the voltage value, and then take off the opaque object to observe the change of the voltage of the multimeter. If the voltage changes obviously, the preamplifier is all right. 6.Check the reference amplifier by using the above method. Check the test point TP204 by the red pen. 7.Further confirmation: block the amplifier, adjust the potentiometer of the preamplifier board, and measure the voltage. If the voltage changes, the preamplifier is all right.
Method 2: indirect measurement 1.Verify the prime amplifier is all right. 2.Enter into the menu of photometric measurement after initialization (if the initialization fails, burst into it). 3.Press F1 to enter into the menu of parameters setting, set the photometric mode to Es, wavelength range to 700-600nm, range 0-100; sampling interval to 1nm. 4.Press RETURN to return to the measurement menu, then press START, setting the gain to 8, and then begin to scan. 5.Open the cover of the sample compartment, block the receiver, verify the energy value, and then take off the opaque object to observe the change of the energy value. If it changes obviously, the sample preamplifier is all right. Adjust the potentiometer of the sample preamplifier. Observe the changes of the energy. If the energy linearly changes with the adjustment of the potentiometer, the preamplifier is all right.
