Manual Detector L-2450 Dad

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10959_Chassis_K16ABaseline_User N-Rhumba_Manual_de_servicio.pdf Manual of

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INSTRUCTION MANU MODEL L-2450 DIODE ARRAY DETECTOR

Please read through this manual carefully and store it in a safe place. •

•

Before using the instrument, instrument, read the safety safety instructions and precautions carefully. Sign up to vote on this title Keep this manual in a safe place place nearby nearby so it  Useful  Not useful can be referred to whenever needed.

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INSTRUCTION MANUAL FOR MODEL L-2450 DIODE ARRAY DETECTOR

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INSTRUCTION MANUAL FOR MODEL L-2450 DIODE ARRAY DETECTOR


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PREFACE

We thank you for purchasing the Hitachi Model L-2450 Dio Array Detector (hereinafter (hereinafter referred to as as DAD). The L-2450 DAD has been specifically designed for detecti change in absorbance in combination with a liquid chromatograph. This instrument is intended for use by persons having a ba knowledge of chemical chemical analysis procedures. Keep in min improper use of analytical instruments, chemicals or sampl would result not only in wrong analytical data but also in consequences adverse to safety. Please read this instruction manual carefully before attemp operation and acquaint yourself with the DAD to enable its correct use. After reading through through this manual, keep keep it handy so it can b referred to whenever needed.

ABOUT THIS MANUAL

This instruction manual has been prepared for the user of t L-2450 DAD, and describes the operating procedures and maintenance/checkup maintenance/checkup instructions for the instrument. First of all, please read “IMPORTANT” and “SAFETY SUMMARY” at the beginning of this manual to ensure safe operation of the instrument. If installation of the instrument is not yet completed, refer to Section 2. When operating the instrument for the first time, read throu Sections 3 and 4 to gain a full understanding of its structure  Sign up to vote on this title basic operation.

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You're Reading a Preview Unlock full access with a free trial.

Download With Free Trial


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IMPORTANT PRECAUTIONS ON CE CONFORMITY MARKING 1.

Electromagnetic Compatibility Requirement

This equipment conforms to the EC Directive 89/336/E and has the CE conformity marking. It is designed satisfy the European Norm EN61326 (1997) and its amendment 1 (1998). This equipment is classified Class A of EN61326. So, this this equipment equipment must not used in domestic establishments nor in establishment directly connected to a low voltage power supply netw which supplies buildings used for domestic purpose. And this equipment equipment is also designed designed to comply with ta “Minimum immunity test requirements” and with evalu table 2 “Continuous unmonitored operation”. If the equipment is used near an intense electromagnetic so however, interfering noise may be given to the instrum to cause an adverse effect on its performance or functionality. 2.

Safety Requirement

This equipment also conforms to the EC Directive 73/23/EEC and has the CE conformity marking. It i designed to satisfy the European Norms EN61010-1: and Am-1 & 2: 1995. This instrument is classified in class 1, pollution degre EN61010-1. Sign up to vote on this title

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Possible Electromagnetic Wave Interference Affec This Instrument

If this instrument is used near an intense electromagn source, interference noise may be given to the instrum to incur an adverse effect on its performance or functionality. To prevent this, use the specified system connection in strict accordance with the t he instruction manual. The instrument is designed to minimize possible electromagnetic wave interference affecting it if the specified cables are connected properly. However, there is no guarantee that electromagnetic interference will not occur in this instrument. If the the instrument does incur electromagnetic electromagnetic wave interferen interfere which can be determined by turning on and off possib sources of electromagnetic wave interference nearby, user is encouraged to try to correct t he interference b or more of the following measures: • •

•

•

Reorient the instrument. Increase separation between the instrument and possible sources of electromagnetic electromagnetic wave interfere Connect the instrument instrument to an outlet outlet on a circuit diff from that to which possible sources of electromagn wave interference are connected. Confirm that any other device device connected connected with with the instrument is not affected by electromagnetic wave interference.


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WARRANTY ON PRODUCT

The L-2450 DAD is warranted to operate according to the specifications given in the instruction manual, provided it in accordance with the instructions described in the manua We make no warranties, either express or implied, as to marketability/merchantability marketability/merchantability and fitness for f or a particular pur pu No oral or written information or advice given by our dealer distributors, agents or their employees without our express permission shall create a warranty or in any way increase t scope of this warranty. (1)

Scope of Warranty

Any parts which prove prove to be defective in design design or workmanship during the warranty period will be repair without charge. A substitute part may be used used for repair, or replaceme replaceme with an equivalent product may be made instead of re Such system components as a personal computer an printer to be updated frequently for improvement may be available in original versions at the t he time of replace Note that this warranty does not apply to the instrume discarded or resold without our, consumables parts an lifetime-expired parts. No reliability is assumed for damage to data or applic software due to any possible fault or failure of the instrument. (2)

Warranty Period  One year from the date Sign of up initial to voteinstallation on this title

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Limitations and Exclusions on Warranty

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(c) Corrosion or deterioration of the tubing due to impurities contained in gas, air or cooling water supplied by the user

(d) Corrosion of the electric circuits or deterioration optical elements due to highly corrosive atmosph gas

(e) Failure due to use of hardware, software or spare other than supplied by us (f)

Failure due to any operation other than described this instruction manual or repair not approved by manufacturer

(g) Failure due to maintenance or repair by a service agent not approved or authorized by us (h) Failure due to relocation or transport without our approval after initial installation

You're Reading a Preview (i) Failure due to disassembly, modification or reloc Unlock full with a free notaccess approved bytrial. us

Download With Free Trialof God, including fire, earthqu (j) Failure due to acts storm, lightning, social disturbance, riot, crime, insurrection, war (declared or undeclared), radioa pollution, contamination with harmful substances (n) Failure of the hardware due to computer virus in (4)

Warranty Card


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INSTALLATION, RELOCATION AND AFTER-SALE TECHNICAL SERVICE (1)

Installation and Relocation

(a) Installation at delivery shall not be carried out by user. It shall be carried out by our service office the engineers who have been trained and qualifie this purpose by us in order to use the instrument and accurately.

(b) Before installation, the user shall make preparati for satisfying the installation requirements in accordance with this instruction manual.

(c) When relocation becomes necessary after initial installation (delivery), please contact the dealer fr whom you purchased it or our service office. (2)

After-sales Service You're Reading a Preview (a) For after-sales service, contact our sales representative or service office.

Unlock full access with a free trial.

Download With Free Trial (b) For service after the warranty period, consult us regard to a maintenance and inspection service contract.


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TECHNICAL SEMINARS AND TRAINING COURSES FOR USERS

We offer technical seminars and training courses at either o user’s facilities to ensure proper and safe operation of the analytical instrument to its full performance. For further information, contact our sales representative. (Applicants be charged.)

CAUTION ON DISPOSAL OF INSTRUMENT

Although at present the instrument does not use materials would directly cause environmental disruption, the environm protection laws and regulations may be revised or amende When planning to dispose of the instrument, therefore, be s check the latest issues of the relevant laws and regulations consult our service office.




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OTHER PRECAUTIONS 1.

Handling of Chemicals and Samples

(1)

The user is responsible for following relevant legal standards and regulations in handling, storage and dis of chemicals and samples used in analytical operation this instrument.

(2)

Reagents, standard solutions and accuracy-control samples shall be handled, stored and discarded as instructed by the respective suppliers.

2.

Notice on This Instruction Manual

(1)

Information contained in this manual is subjected to without notice for product improvement.

(2)

This manual is copyrighted by Hitachi High-Technolo Corporation with all rights reserved.

You're Reading a Preview (3) No part of this manual may be reproduced or transmit Unlock full access with a free trial. any form or by any means without the express written permission of Hitachi High-Technologies Corporation. Download With Free Trial


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SAFETY SUMMARY

Before using the Hitachi Model L-2450 DAD, be sure to rea following safety instructions carefully.

General Safety Guidelines •

•

•

Follow all the operating procedures provided in the instr manual for each instrument. Installation at delivery shall be carried out by the engine who have been trained and qualified for this purpose by It shall not be carried out by the user. Be sure to observe the warnings indicated on the instrum and in the instruction manual. Failure to do so could re personal injury or damage to the instrument.

The hazard warnings which appear on the warning labels o product or in the manual have one of the following alert hea You're Reading a Preview consisting of an safety alert symbol and signal word DANG Unlock full access with a free trial. WARNING or CAUTION.

Download With Free Trial DANGER : Indicates an imminently hazardous situation which, if not avoided, will res in death or serious injury. WARNING : Indicates a potentially hazardous situation which, if not avoided, can re in death or serious injury. CAUTION : Indicates a hazardous situation which  Sign up to vote this title not avoided, canon result in minor or moderate  Usefulinjury.  Not useful : Safety alert symbol used for calling attention to a potential hazard which

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SAFETY SUMMARY General Safety Guidelines (Continued)

In addition to the above, the following signal word is used to indicate instructions for ensuring proper use of the product. NOTE:

Indicates an instruction for ensuring correct the product and accurate analysis therewith.




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SAFETY SUMMARY General Safety Guidelines (Continued) Precautions on Use •

Before using this instrument, be sure to read the instruc manual carefully to acquire a full understanding of its functions and operations.

•

Keep the instruction manual in a safe place nearby so th can be referred to whenever needed.

•

Be sure to follow the operating procedures and usage described in the instruction manual.

•

Be sure to understand and follow all the safety instructio and precautions in this manual. You're Reading given a Preview

•

Unlock full access with a free trial. Observe all of the above instructions. Failure to do so result not only in wrong analytical data or but also in per Download With Free Trial injury.

•

Do not modify the instrument, use non-specified parts, n remove safety devices, as it could be hazardous.

•

When using a chemical for analytical operation, be sure provide proper ventilation in the laboratory room as per  requirements. Inadequate could Sign upventilation to vote on this title endanger health. Useful Not useful

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SAFETY SUMMARY General Safety Guidelines (Continued) Precautions on Installation, Maintenance and Relocation •

Installation at delivery shall not be carried out by the us It shall be carried out by our service office or the engine who have been trained and qualified for this purpose by order to use the instrument safely and accurately.

•

At installation, check if any standard accessory is missin If missing or damaged, or if you have any question, then contact your nearest sales representative. If the instrument is operated without any standard acces equipped, it may malfunction to cause a hazardous cond In such a case, follow the instructions given by the insta engineer. You're Reading a Preview

•

Unlock full access with a free trial. Do not modify the instrument, replace parts that are not serviceable, use non-specified parts, nor remove safety Download Free devices, as itWith could beTrial hazardous.

•

Installation at delivery, maintenance and relocation sho referred to service personnel qualified by us.

•

Do not perform any operation or action other than descr in the instruction manual. When in doubt, please conta or our dealer from whom you Sign purchased instrument up to votethe on this title service office. Useful Not useful

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

Keep in mind that the hazard warnings in the instruction

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SAFETY SUMMARY General Safety Guidelines (Continued) •

Maintenance to be performed by the user should be with scope described in this instruction manual. Before proceeding to maintenance, be sure to attain a c understanding of maintenance items described in the instruction manual. Do not carry out any maintenance other than described in the instruction manual. Failure so may cause trouble in the instrument or personal injur

•

Avoid relocating the instrument after installation. The precisely adjusted mechanical system may be adve affected by possible vibration during relocation.

•

If any warning label is deteriorated due to long use or damaged, contact our service office. You're Reading a Preview Unlock full access with a free trial.



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SAFETY SUMMARY WARNING: Ignition of Flammable Chemicals! Exercise utmost care when handling flammable chemicals. •

Beware of ignition hazard when using flammable chemic such as organic solvents.

•

Always check the flow path for the following points. abnormality is found, stop operation immediately. ◇ Leakage of solvent used or waste solution ◇ Leakage of solvent inside the instrument

•

•

•

Well-ventilate the laboratory room where the instrument used.

You're Reading a Preview This instrument is not of an explosion-proof type. Do Unlock full access with a free trial. organic solvents having an ignition point below 70 °C in unattended operation. Download With Free Trial

When using flammable chemicals, be careful about po ignition due to static electricity. Particularly when using conductive chemicals, employ a conductive vessel and provide a correct grounding connection.

 Sign up to vote on this title WARNING: Explosion of Vapor from Flammable  Useful  Not useful Chemicals!

Exercise utmost care when handling flammable chemicals.

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SAFETY SUMMARY

WARNING: Electric Shock due to Contact with Ins of Instrument! Beware of electric shock. the instrument.

Potentially dangerous voltages are present wit

Before removing the instrument cover for replacement of p any other work, be sure to turn off the power switch and un the power cord.

WARNING: Electric Shock due to Improper Grounding! Ground properly to prevent an electric shock hazard. You're Reading a Preview

•

Unlock free trial.cable supplied with the instrum Be surefulltoaccess use with the apower Use of a different power cable may result in an electric s Download With Free Trial hazard.

•

This instrument is of a “plug-connected type” specified i European Norm EN61010-1. So connect the power a grounded three-wire outlet.

•

When a grounded three-wired outlet is not available, th power cable may be connected to a two-wire outlet  throu Sign up to vote on this title conversion adapter. In this case, be sure to provide  Useful  Not useful grounding connection.

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SAFETY SUMMARY CAUTION:

Burns due to Contact with Hot Part!

Exercise utmost care when replacing the light source lamp! •

The lamp and lamp cover are hot during use or immedia after use. Touching the hot lamp cover or lamp could in burns.

•

Before lamp replacement, turn off the power switch and for 30 minutes until the lamp cools down sufficiently.




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SAFETY SUMMARY NOTICES Precautions on Use of Reagents

Fluorocarbon resin and quartz materials are used for the w parts of the instrument. Never use solvents that would co these materials.

Precautions on Use of Corrosive Solvents •

The drain path for carrying leakage solutions is made of polypropylene.

•

The materials inside the instrument are susceptible to corrosion by strong acid, strong alkali and organic solve You're Reading a Preview

•

•

Unlock using full access with a freesolvents, trial. When corrosive make sure that the tubin connections are not loose. Download With Free Trial

Using the pump pressure limiter function or by some oth means, make setting so that liquid delivery is stopped automatically if leakage occurs.

Precautions on Disposal of Waste Solution Sign up to vote on this title



Be sure to collect waste solution and treat it properly for dis  Useful  Not useful according to the relevant laws and regulations. Improper disposal treatment of waste solution may result in

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SAFETY SUMMARY Precaution on High Pressure

In the liquid chromatograph, high pressure is applied to its path during operation. Before attempting operation, make that there is no leakage from the flow path. And when usi harmful solvent, be sure to wear safety goggles.

Prevention of Instrument Tipover

Provide a proper means for preventing the instrument from tipping over accidentally.

Precaution on Waste Solution Bottle

You're Reading a Preview Prepare a waste solution bottle having a sufficient capacity Unlock full with aquantity free trial. of mobile phase at least). (sufficient foraccess the total Download With Free Trial


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SAFETY SUMMARY WARNING LABELS

The warning labels shown below are attached to the L-2450 DAD. Read the warning labels carefully and check the instruction them to acquire a clear understanding with reference to act parts. Periodically check if these warning labels are clean to allow reading over a safe distance. If any one of them becomes illegible due to deterioration our service office for replacement with a new one. (1)

Explosion of Vapor from Flammable Chemicals

WARNING

You're Reading a Preview

Beware of explosion! Unlock full access with a free trial.

Attached of left sid

Can cause serious injury. High concentration of Download With Free Trial organic solvent vapor may lead to explosion. Do not allow flammable chemicals to leak. 810-1952

(2)

Ignition of Flammable Chemicals


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SAFETY SUMMARY (3)

Electric Shock due to Contact with Power Supply to L Source

WARNING Beware of high voltage : 500V. Can cause fatal or serious injury.

Attached right

Before removing light source cover, turn power switch off. Never release safety mechanism inadvertently. 810-1958

You're Reading a Preview (4)

Unlock fulldue access a free trial. Burns towith Contact with

Hot Part


CAUTION

Beware of high temperature! Can cause burns. Light source lamp and lamp chamber remain hot for a while even after power-off Before lamp replacement, turn Sign up to vote power off and wait untilon it this title Useful  Not useful becomes sufficiently cool. 810-1962

Attached right

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CONTENTS

PREFACE .......................................................................................................................

ABOUT THIS MANUAL .................................................................................................

IMPORTANT ................................................................................................................... PRECAUTIONS ON ELECTROMAGNETIC WAVE INTERFERENCE ................................................................ WARRANTY ON PRODUCT .............................................. INSTALLATION, RELOCATION AND AFTER-SALE TECHNICAL SERVICE....................... ......... TECHNICAL SEMINARS AND TRAINING COURSES FOR USERS .................................. CAUTION ON DISPOSAL OF INSTRUMENT .................... OTHER PRECAUTIONS.....................................................

SAFETY SUMMARY ......................................................................................SAFET General Safety Guidelines...................................SAFET Precautions on Use...................................................SAFET You're Reading a Preview WARNING: Ignition of Flammable Chemicals!....SAFET Unlock full access with a free trial. WARNING: Explosion of Vapor from Flammable Chemicals! ....................SAFET Download With Free Trial WARNING: Electric Shock due to Contact with Inside of Instrument! ........................SAFET WARNING: Electric Shock due to Improper Grounding!........................SAFET CAUTION: Burns due to Contact with Hot Part!...........................................SAFE NOTICES .................................................................. SAFE  SAFE WARNING LABELS .................................................. Sign up to vote on this title

 1.

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OUTLINE.................................................................................................................. 1.1 Isocratic System......................................................

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Assembly ................................................................ 2.6.1 System Power Supply............................ 2.6.2 System Wiring............................................ 2.6.3 System Piping ............................................ 2.6.4 Instrument-to-instrument Fastening ............ 2.6.5 Removal of Transport Screw and Sponge................................................

3.

FUNCTION ............................................................................................................... 3.1 Front Panel............................................................... 3.2 Rear Panel............................................................... 3.3 Operating Principle ................................................. 3.4 Specifications ..........................................................

4.

OPERATION ............................................................................................................ 4.1 Turning On Power ................................................... 4.2 Cautions on Operation............................................ 4.3 Shutdown Procedure .............................................. 4.4 You're Washing after Use ................................................... Reading a Preview

5.

Unlock full access with a free trial. ........................................................................ MAINTENANCE AND INSPECTION 5.1 Performance Check at Installation ....................... Download With Free Trial 5.1.1 Operation Check .............................. .......... 5.1.2 Performance Check ................................... 5.2 Periodical Inspection .............................................. 5.3 Maintenance/Inspection Procedure....................... 5.3.1 Check and Washing of Flow Cell ................ 5.3.2 Disassembly and Washing of Flow Cell...... 5.4 Troubleshooting ......................................................  5.5 Error Messages ......................................................


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REPLACEMENT PARTS .........................................................................................

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OUTLINE

The liquid chromatograph system consists of a pump for feeding an eluent, a sample introducing section (autosam or manual injector) for injecting a sample, a column for chromatographic separation of the injected sample, a col oven for maintaining the column at a constant temperatu and a detector section for detecting each separated component of the sample as a signal. If necessary, a degasser for deaerating an eluent and/or a gradient devi varying the mixing ratio of eluents with time can also be incorporated in the system.

1.1

Isocratic System This is a simple system in which analysis is made with a solvent.

You're Reading a Preview Washing solution

Eluent



Organizer

L-2450 DAD

L-2300 Column Oven Column


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L-2200 Autosampler

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Isocratic System

(1)

An eluent is fed by the pump through the degasser.

(2)

A sample is injected by the autosampler.

(3)

The injected sampler is separated through the colum whose temperature is kept constant by the column o

(4)

Each separated component of the sample is detecte a signal by the detector.




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Low-pressure Gradient System

In the low-pressure gradient system, two or more solvent mixed in the low-pressure section and then fed by a sing pump. The mixing ratio is made to vary with time in chromatographic separation. Washing solution

Eluent

Organizer

L-2450 DAD

図ｶﾗﾑ Column

L-2300 Column Oven


L-2200 Autosampler

Download With Free Trial Mixer L-2100/2130 Pump Degasser Drain Low-pressure gradient unit Sign up to vote on this title

 (1)

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Each eluent is aspirated from the degasser into the

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Low-pressure Gradient System

(5)

The injected sample is separated through the colum whose temperature is kept constant by the column o

(6)

Each separated component of the sample is detecte a signal by the detector.




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High-pressure Gradient System

In the high-pressure gradient system, two or more solven are fed by multiple pumps and mixed in the high-pressure section for liquid delivery. Washing solution

Eluent

L-2450 D

L-2300 C Oven

Organizer Column

L-2100/2130 Pump L-2200 Autosa


Degasser

Mixer


Drain

(1) From the degasser, eluents are aspirated into each (2)

(3)

The eluents are fed from the pump at a flow rate corresponding to the preset mixing ratio. Sign up to vote on this title



The eluents thus fed are mixed at the mixer and the  Useful  Not useful to the next section.

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INSTALLATION

Unpacking

Unpack the shipping crate carefully while avoiding shock take out the main unit and standard accessories, and pla them on a sturdy and level desk or bench. NOTE:

Installation at delivery shall not be carried out user. It shall be carried out by our service offi the engineers who have been trained and quali for this purpose by us in order to use the instru safely and accurately. Before installation, the user shall make prepara for satisfying the installation requirements in accordance with this instruction manual. When relocation of the instrument becomes necessary after initial installation (delivery), ple notify the dealer from whom you purchased it o You'reservice Readingoffice. a Preview




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Installation Conditions

Installation Conditions Confirm the following requirements before installing the instrument. (1)

Power Supply Line voltage

:

100 to 240 V AC Fluctuation should be within ±10% the rated voltage.

Frequency

:

50 or 60 Hz Fluctuation should be within ±0.5 the rated frequency.

Power capacity :

200 VA or more for L-2450 DAD 500 VA or more for total system Provide power supply sufficient fo combinational use with other dev

Connection : Socket connection You're Reading a Preview Power supply shall be available n Unlock full access with a free trial. the installation place for the syste including this instrument. Download With Free Grounded Trial three-wire outlet (4 pla

WARNING

Ground Properly to Prevent Electric Shock Hazar •

•

Be sure to use the power cord supplied with the  up to votepower on this title instrument. Use of Sign a different cable may res Useful  Not useful in an electric shock  hazard. This instrument is of a “plug-connected type” specifie in European Norm EN61010-1. So connect the pow

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(2)

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Ground Terminal

It is required to provide a grounding connection hav resistance of 100 Ω or less within a distance of 3 m the instrument. Table tap with ground cable Power outlet

AC adapter Grounding resistance: 100 Ω or less

750 mm or more underground

e t a l p r e p p o C

Ground terminal

Ground wire (green) Underground

300 mm

2 mm

Connec


10 mm

Download With Free Trial Fig. 2-1

2.3

Grounding Connection

Installation Space  For installation of the L-2000 series unit, it is required to Sign up to vote on this title provide a space of 640 mm (W) × 550 mm (D) or more.  Useful  Not useful Also provide a space of at least 100 mm at the rear and sides of the unit.

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Installation Environment

Installation Environment

When selecting an installation place for the instrument, b sure to check the following conditions. (1)

Operating Temperature

15 to 35 °C Temperature variation during measurement should minimized. Take utmost care not to allow condens (2)

Operating Humidity 45 to 85%

(3)

Atmosphere (a) Well ventilated. (b) Free from acidic or alkaline gases that could significantly. You'remetals Reading a Preview Unlock access withorganic a free trial.solvent (c) full Free from

gases (particularly benzene and thinner) that could affect the pain Download With coating onFree the Trial instrument .

(4)

Other Requirements

(a) Not exposed to direct sunlight. Exposure to direct sunlight could result in deterioration in performance or discoloration of  instrument. Avoid installing the instrument ne Sign up to vote on this title window of the laboratory room.

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(b) Not exposed to drafts directly.

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Free from excessive dust. Contamination with dust could deteriorate the performance of the instrument.

(g) No abrupt fluctuation in line voltage. Fluctuation in line voltage could cause noise in analytical data.

(h) Avoid frequent ON/OFF operations of an electr device not equipped with a noise suppressor ci (stirrer, vibrator, etc.) on the same power line a this instrument.

2.5

Check of Delivered Contents After completion of unpacking, check the delivered cont against the packing list supplied with the instrument. part is missing or damaged, or if you have any question, contact ourReading sales representative or service office. You're a Preview Unlock full access with a free trial.



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Assembly

Assembly

2.6.1 System Power Supply

An organizer is available for the L-2000 series system. Connectable to the organizer (item to be prepared separ are two pumps, one autosampler and one detector. The column over has its own power cord. In a situation where two detectors are provided with a single organizer installed, use a proper AC adapter (option). The power from the organizer and AC adapter power cable should b connected to the power connector at the left rear side of instrument.



Power connector


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Isocratic System/Low-pressure Gradient System

Organizer

Square con cable L-2450 DAD

L-2300 Column Oven

L-2200 Autosampler

Round con cable


L-2100/2130 Pump Unlock full access with a free trial.

Download With Free Trial To AC power source

Connect the respective power cables of the organiz and column oven to the AC power source. For the  conne L-2100/2130 Pump and L-2200 Autosampler, Sign up to vote on this title round connector cables from the organizer to their p  Useful  Not useful connectors. For the L-2450 DAD, connect the squ connector cable from the organizer to its power

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Assembly

(2)


Square connector cable L-2450 DAD Organizer L-2300 Column Oven

Round con cable L-2200 Autosampler

L-2100/2130 Pump


To AC power source


Connect the respective power cables of the organiz Download With FreetoTrial and column oven the AC power source. For the L-2100/2130 Pump and L-2200 Autosampler, conne round connector cables from the organizer to their p connectors. For the L-2450 DAD, the square conn cable from the organizer to its power connector.


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2.6.2 System Wiring

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In the L-2000 series, connect the e-Line cable to the e-Li connector equipped on the left rear of the instrument. Connect the D-line cable between the synchronous seria communication connector of the L-2450 DAD and the US interface board.

e-Line connector

(1)

Synchronous communicatio connector

Isocratic/Low-pressure Gradient System

Organizer

L-2450 DAD e-Line cable L-2300 Column Oven


 L-2200

Useful

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Assembly

(2)


e-Line cable L-2450 DAD

Organizer

L-2300 Column Oven

e-Line cable

L-2200 Autosampler Autos ampler

L-2100/2130 Pump

2.6.3 System Piping

In the L-2000 series, provide inlet tubing to the pump and waste solution tubing through the right piping section of t drain reservoir, and tubing from the pump to the autosam column and detector through the center piping section of drain reservoir.

Center piping section Run tubes from the pump to the autosampler, column and detec

Drain reservoir


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Right piping section

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Isocratic System Washing solution

Eluent

Inlet tube Organizer

Detector ce L-2450 DAD


L-2200 Autosampler Autosa mpler

Injection va

Syringe valv

L-2100/2130 Pump

Drain Degasser

Drain valve


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

Connect the inlet tube for eluent to the IN port of the degasser. Connect the OUT port of the degasser

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Assembly

(2)

Low-pressure Gradient System Washing solution

Eluent

Inlet tube Organizer

Detector ce L-2450 DAD


Injection va

Syringe va L-2200 Autosampler


Download Mixer With Free Trial

L-2100/213 Pump

Degasser

Drain valve

4-way joint

Drain

Proportioning valve Sign up to vote on this title



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Connect the inlet tube for eluent to the IN port of the

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High-pressure Gradient System Inlet tube

Detector cell

Eluent L-2450 DAD

Washing solution

L-2300 Column Oven

Organizer

Injection valv

Column

Syringe valv L-2100/2130 Pump

L-2200 Autosampler

Drain Drain valve


Connect the inlet tube for eluent to the IN port of the Download Free Trial degasser.With Connect the OUT port of the degasser IN port of the pump. Connect the drain valve of pump to the mixer. Connect of the mixer of the port 1 of the injection valve of the autosampler. Connect port 2 of the injection valve to the inlet of th column. Connect the outlet of the column to the tube of the detector cell. For washing solution, pro  connection to port 1 of the syringe valve of the Sign up to vote on this title autosampler through the degasser.

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Assembly

2.6.4

Instrument-toinstrument Fastening

The wiring cover is provided at the rear of each instrume The instrument-to-instrument fastening can be made by shifting each wiring cover as shown below. (1)

Loosen the retaining screws and detach the wiring

Wiring co

Retaining screw

(2)

Shift the wiring cover and tighten the retaining screw



Wiring cov


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Retaining 

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2.6.5 Removal of Transport Screw and Sponge

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Two transport screws are provided at the bottom of instrument. Remove them with a Phillips screwdriv


Transport screw


The transport sponge is provided inside the instrum Download With Free Trial Detach the lamp cover at the front of the instrument Then, detach the Hg lamp lid and remove the transp sponge (1 pc). For attachment of the Hg lamp, ref 6.2.3.


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FUNCTION

Front Panel Power switch

LED


Fig. 3-1

Front Panel

Download With Free Trial Power switch : LED :

Turns ON/OFF power. Four LEDs are equipped, which provide status indications given below.

Name (color) CH1 (green/orange) CH2 (green/orange) D2 LAMP (green)

LED Status Green : Use of CH1  Orange Error in use Sign up to: vote on this title of CH1 Use CH2 Useful :  Notofuseful Green Orange : Error in use of CH2 Green :

D 2 lamp ON

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Rear Panel

Rear Panel

e-Line connector

Synchronous serial communication connector

Power connecto

Fig. 3-2 Rear Panel You're Reading a Preview Unlock full access with a free trial.

Download With Free Trial No. 1

Indication e-Line connector

Function (1) Sends/receives instrument parameters through e-Line. (2) Inputs/outputs contact signals. (a) START IN (b) ERROR IN

Reference (1) Sending/receiving of instrument parameters

(2) (a) Start of program (b) Recognition of erro other instrument  of erro (c) ERROR OUT (c) Presentation Sign up to vote on this title condition Useful Not useful   (d) READY/BUSY OUT (d) Presentation of sta

2

Synchronous serial

Used to send acquired data to USB interface.

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Operating Principle

W lam

Lens

Converging mirror

Flow cell

Photodiode array

Lens Switching mirror Slit



D2 lamp

Hg lamp

Grating

Fig. 3-3

Optical System SignDiagram up to vote on this title

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Figure 3-3 shows the optical system of the L-2450 DAD.

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Specifications

Specifications (1)

Optical System

Single beam photometry system (dispersion by diffr grating) (2)

Light Source D2 lamp, W lamp, Hg lamp (for wavelength check)

(3)

Detecting Element 1024-bit photodiode array

(4)

Measuring Wavelength Range 190 to 900 nm (settable in steps of 1 nm)

(5)

Wavelength Accuracy

You're Reading a Preview ± 1 nm Unlock full access with a free trial.

(6)

Slit Type Download With Free Trial 2 types (FINE: 1 nm, COARSE: 4 nm)

(7)

Noise

1.5 × 10 -5 AU or less (Conditions: Air in cell, spectral bandwidth 4 nm, smoothed chromatogram 250± 10 nm Sign up to vote on this title 600 ± 10 nm, sampling period 400 ms  Useful  Not useful constant 1.5 s, ambient temperature 2 ambient temperature variation within

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Communication Function e-Line (instrument parameters, instrument status) Synchronous serial communication (acquired data)

(10) External Output Terminal (option) Processor output (1 V in full scale) Recorder output (10 mV in full scale) (11) External Input/Output Contact Terminal

Time program start, error in/out, busy out (contact o Line) (12) Flow Cell Quartz cell (optical path length 10 mm, cell capacity 13 µL) (13) Withstand Pressure of Flow Cell You're Reading a Preview 1.0 MPa Unlock full access with a free trial.

(14) Operating Temperature Range Download With Free Trial 4 to 35 °C (condensation unallowable) (15) Operating Humidity Range 45 to 85% (condensation unallowable) (16) Power Supply 24 V DA


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OPERATION

Turning On Power Turn on the power switch of the instrument. Power switch



Upon powering on the instrument, a self-diagnosis (initialization) test is made. NOTE:

If an error message appears after turning on then refer to 5.4 and 5.5. Sign up to vote on this title

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Cautions on Operation

Cautions on Operation

For the L-2450 DAD, data acquisition parameters, photometric values and error information are all controlled/managed by the PC (EZChrom Elite for Hitach For use of the instrument, it is necessary to make setting the PDA option in the Set Option window. (For details, to the instruction manual for EZChrom Elite for Hitachi (c option). A license is required separately for the threedimensional analysis option.) In three-dimensional data acquisition, spectral data is sequentially transferred from the L-2450 DAD to the PC. During data acquisition, you can monitor a chromatogram real time on the PC. After completion of each analysis, dimensional data is stored in the hard disk of the PC. The three-dimensional chromatogram is used for peak pu check, peak component identification or library data preparation/search with the contour display and threedimensional chromatogram display functions on the PC s In addition, an ordinary chromatogram is selected and quantitative calculation is carried out for peaks in the You're Reading a Preview chromatogram. For the function and operation of EZCh full accessrefer with a to freeits trial. EliteUnlock for Hitachi, instruction manual.

Download With Free Trial NOTE: If absorbance becomes high in absorptio metr the accuracy of photometric values will fall. Linearity should be corrected by using a worki curve when analyzing samples of over 1 AU. The linearity range may become narrower if a eluent of large absorption is used.

NOTE: There are gaps between the cut filtersat arou Signnm up toinvote this title 350 nm and 580 thisonproduct. Since Useful here Not useful light volume  is lower at other  than wavelengths, steps may appear on a spectrum in the following cases.

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Shutdown Procedure

To shut down the instrument, turn off its power switch. Note that the instrument should be shut down after termi of EZChrom Elite for Hitachi. The set measuring conditions are saved even when powe turned on. Upon turning on power, you can make measurement under the measuring conditions saved bef turning off power.

4.4

Washing after Use

After use of buffer solution, be sure to wash the flow cel distilled water. Otherwise the flow cell might be damage due to clogging with deposited salt.




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MAINTENANCE AND INSPECTION

Performance Check at Installation

5.1.1 Operation Check (1)

5.1.2


Performance Check

Install the instrument according to Section 2.

(2)

Turn on the power switch of the instrument and mak sure that the CH1 or CH2 LED is lit.

(3)

Make sure that the D2 LAMP and W LAMP LEDs a

(4)

Start up EZChrom Elite for Hitachi and in the Instrum Status window, make sure that the DAD is in Ready status.

Performance check at installation should be carried out u the following conditions. • Install the instrument in a room where the temperature toYou're 30 °CReading and theatemperature variation is within ±1 ° Preview place where the instrument is not exposed to draft from Unlock full access with a free trial. air-conditioner or the like). • Warm up the instrument for at least one hour after tur Download With Free Trial on power. • Fill the flow cell with air. NOTE:

If the inside of the flow cell is contaminated dried completely, the instrument performanc not be checked correctly. If the flow cell contaminated, wash it (refer to 5.3) before u Sign up to vote on this title



which is small • For check of the noise Useful use Not useful level, data baseline drift.

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(d) Click the Maintenance button.


(e) Click the WL Check button. Download With Free Trial


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(f)


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Select wavelengths to be checked and click the Check Start button.


(g) Wavelength check is automatically carried out Download With Free Trial the wavelength deviation is displayed for each selected wavelengths.


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NOTE:

(2)

If any wavelength is outside the calib range (indicated in blank) in waveleng check results, set the calibration valu the default one (83) in the calibration setting window and retry wavelength accuracy check. (For details, refer instruction manual for the maintenanc software.) If a wavelength outside calibration range is found again, cont our service office.

Check of Lamp Energy

(a) Click the Get button, and the respective energy values of the D2 lamp and W lamp are displaye




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(3)


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Check of Drift and Noise

For drift and noise check, use EZChrom Elite for Hit Upon completion of automatic configuration of Hitac LaChrom Elite, the automatically detected modules appear in the Used Modules field. (For the detaile operating procedure, refer to the instruction manual EZChrom Elite for Hitachi (control option).) For use of the PDA option software, click the Optio button.



Select PDA in the Set Option window.


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(a) Check the drift in the following procedure. 1) Open the Instrument Parameters dialog click the DAD tab.


2)

Set the following conditions in the Instrume window. DownloadParameters With Free Trial Sampling time : 60 min Sampling period : 400 ms Wavelength range : 200 to 700 nm Slit width : Coarse Band width : 4 nm 3) Set the following data processing condition the PDA Option window.  Sign up to vote on this title Multi-chromatogram: useful  Useful  Not250 Channel 1: Wavelength nm, band 20 nm Channel 2: Wavelength 600 band

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5)

e c n a b Time r o s b A


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Calculate the drift at wavelengths of 250 600 nm.

axis

60 minutes

(b) Check the noise level in the following procedur 1) Set the following conditions in the Instrume Parameters window. Sampling time : 15 min You're Reading a Preview Sampling period : 400 ms Unlock full access with a free trial. Wavelength range : 200 to 700 nm Slit width : Coarse DownloadBand With Free Trial width : 4 nm 2) Set the following data processing condition the PDA Option window. Multi-chromatogram: Channel 1: Wavelength 250 nm, band 20 nm Channel 2: Wavelength 600 nm, band  20 nm Sign up to vote on this title 3) Execute data acquisition and prepare  Useful  Not useful chromatograms at wavelengths of 250 nm 600 nm.

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Periodical Inspection

R =n

Noise level = ∑ YR n

(Xn is in units of 0.5

R=1

Noise calculation (cited from ASTM E685-7 X3

X1

CENTER

X2

Y2 Y3 Y1 15 minutes

5.2 No.

Periodical Inspection Check Item

Interval

Check Procedure

Remar

1

Lighting of light source lamp

Every day

Check if D2 LAMP and W LAMP LEDs come on.

2

Lifetime of light source lamp

Every 1) Make energy check and ON month Unlock full accesstime with acheck. free trial. 2) Check if baseline drifts largely (or baseline fluctuates or noise becomes Download With Free Trial large).

See 5.1.2.

3

Wavelength accuracy

Every year

See 5.1.2.


Check wavelength by utilizing Hg lamp and D 2 lamp bright lines.


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Maintenance/Inspection Procedure

5.3.1 Check and Washing of Flow Cell

WARNING Ignition of Flammable Chemicals! • Beware of ignition hazard when using flammable •

• •

•

chemicals such as organic solvents. Always check the flow path for the following points. If an abnormality is found, stop operation immediatel ◇ Leakage of solvent or waste solution ◇ Leakage of solvent inside the instrument Well-ventilate the laboratory room where the instrum is used. This instrument is not of an explosion-proof type. Do not use organic solvents having an ignition point below 70 °C in unattended operation. When using flammable chemicals, be careful about possible ignition due to static electricity. Particularly when using non-conductive chemicals, You're Reading a Preview employ a conductive vessel and provide a correct grounding connection. Unlock full access with a free trial.


WARNING

Explosion of Vapor from Flammable Chemicals!

• If a flammable chemical such as organic solvent leak

from the flow path of the instrument and its vapor  concentration exceeds explosion Signthe up to vote on thislimit, title it may cau spontaneous combustion with  dangerously Not useful explosive  Useful results. • When using a flammable and readily volatile chemica

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(1)

If the inside of the flow cell is contaminated, it shou washed.

Items to be prepared for washing flow cell: • Injector (made of glass and having a capacity of about 10 • Washing solution (2)

Check and wash the flow cell in the following proced

(a) Loosen the two setscrews and remove the flow cover. Setscrew


Download With Free Trial Flow cell cover

(b) Loosen the two setscrews and remove the flow lid. Setscrews Sign up to vote on this title

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Flow cell unit

Flow cell

(c) Pull out the flow cell spacer toward the front of You'redetector. Reading a Preview Unlock full access with a free trial.

Flow cell spacer



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(d) Pinch the center of the flow cell unit with your f and with the flow cell pressed against the right source side) of the instrument, take out the flow by pulling it toward the front of the instrument.

Pressing dire

You're Reading a Preview Pulling direction


(e) Visually check the inside of the flow cell for adh Download With Free Trial of foreign matter (crystallized matter or the like) contamination. (f)

Visually check the flow cell for liquid leakage.

(g) If the inside of the flow cell is contaminated, it be washed. The washing solution to be used  depending on the used. the elue Signeluent up to vote on this When title an aqueous liquid, insert theNot injector useful filled with  Useful distilled water into the tip of the drain tube and the water into the cell for washing. When the

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Drain tube



Connecting tube (1.6 to 2 mm dia.)

Injector

(h) Replace the distilled water or ethanol (acetone the eluent used and take the same procedure a step (f). (i)

If air bubbles are included in the flow cell, pu liquid into it at a flow rate of 1.0 mL/min and a pressure of 1.0 MPa or less, with the outlet of t drain tube stopped up with your finger. Under You'restatus, Reading a Previewthe cell for 2 or 3 seconds an pressurize then release thetrial. finger from the drain tube. Unlock full access with a free Air bubbles can be eliminated by repeating thi procedure 3 orTrial 4 times. Download With Free


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(j)

Insert the flow cell straight from the front of the instrument and set it at a position where the protrusion of the cell holder is retained in the ho inside the instrument (with a click).


Cell holder


Protrusion

NOTE:




Set the flow cell at the correct position.  Useful  Not useful Otherwise the instrument performance may be degraded.

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5.3.2 Disassembly and Washing of Flow Cell


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WARNING Ignition of Flammable Chemicals! • Beware of ignition hazard when using flammable •

• •

•

chemicals such as organic solvents. Always check the flow path for the following points. If an abnormality is found, stop operation immediatel ◇ Leakage of solvent or waste solution ◇ Leakage of solvent inside the instrument Well-ventilate the laboratory room where the instrum is used. This instrument is not of an explosion-proof type. Do not use organic solvents having an ignition point below 70 °C in unattended operation. When using flammable chemicals, be careful about possible ignition due to static electricity. Particularly when using non-conductive chemicals, employ a conductive vessel and provide a correct grounding connection. You're Reading a Preview Unlock full access with a free trial.

WARNING

Download With Free Trial Explosion of Vapor from Flammable Chemicals!

• If a flammable chemical such as organic solvent leak

from the flow path of the instrument and its vapor concentration exceeds the explosion limit, it may cau spontaneous combustion with dangerously explosive results.  Sign upand to vote on thisvolatile title readily chemica • When using a flammable Useful from useful  Not be sure to check for leakage the flow path of th instrument and ventilate the laboratory room adequately.

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If the flow cell is still contaminated after washing, disasse and wash it in the following procedure. Retaining screw

Setscrew 1 080-3290

Seat 810-2697

Seat 810-2697

Setscrew 2 080-3290


(1) (2)

Loosen setscrews 1 and 2. Download With Free Trial Loosen the retaining screws.


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O-ring L456005

Flow cell 10 mm 890-2394

Packing 060-3076

Cell holder 890-2221

Reading a Preview (3) You're Take out the flow cell. (4) (5)

(6)


Wash the flow cell with an ultrasonic cleaner.

Download With Free Trial Wipe off contamination from the surface of the flow using gauze moistened with acetone or ethanol.

Align the upper and lower flow path holes in the flow with the upper and lower screw holes in the cell hold Then insert the flow cell into the cell holder. Sign up to vote on this title

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(7)

Reassemble the flow cell by reversing the disassem procedure.

(8)

Flow liquid to check for leakage. NOTE:

Tighten the setscrews fully by hand and retighten them by about 30 ° with a double wrench. Before check for liquid leakage sure to set the pressure limiter of the pum 1 MPa.




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Troubleshooting

For operation and maintenance/inspection of the persona computer (PC), printer and HPLC unit, refer to their instru manuals. Described here is the troubleshooting of the L-2450 DAD Symptom 1. CH1 (or CH2) LED does not come on when power switch is turned on.

2. D2 LAMP (or W LAMP) LED does not come on.

Cause

Judgement/ Check Procedure

Remedy

1) Poor contact of power cord plug (system power unit or AC adapter)

Visual check

Plug in power again.

2) Improper attachment of lamp cover (activation of protective circuit)

Visual check

Retighten retainin screw of lamp cov

1) Improper connection of lead wire of light source lamp

Visual check

Reconnect lead light source lamp according to indic on terminal block

2) Blowout of light source lamp

Check light source lamp for conductivity.

Replace light sou lamp.

3. CH1 (or CH2) is lit in orange.

SYSTEM error

Turn off power and then turn it on again. If same symptom You're Reading aappears, Previewstart up maintenance software of EZChrom Elite for Unlock full access withHitachi a free trial. and check error message.

Refer to error me list.

4. Noise is too large.

1) Contamination ofWith Free Visual check Download Trial flow cell

Wash flow cell 5.3.3).

2) Insufficient deaeration of solvent

Appea rance of sp ikelike noise

Deaerate solvent sufficiently.

3) Impurities contained in solvent

Check solvent.

Purify or replace solvent.

4) Trouble on pump

Check for regularity.

Check pump.

Check for noise when pump is stopped. 5) Expiration of lamp life (D2/W) 5. Drift is too large.

1) Insufficient warming-up

Make energy check of Replace lamp wit  lamp Sign (seeup 5.3.1). to vote on this new title one (see 6.2

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Wait until instrum

 Not useful becomes stable ( hour or more).

2) Liquid leakage from

Check joint for poor

Retighten each jo

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Error Messages

Error Messages

On occurrence of an abnormality in operation of the L-24 diode array detector, an error message is reported on the display monitor. Message

Description

Remedy

ROM ERROR message has been received from the L-2450 DAD. Close the instrument icon and restart the unit.

ROM error

Contact the service office.

RAM ERROR message has been received from the L-2450 DAD. Close the instrument icon and restart the unit.

RAM error

Contact the service office.

PARAMETER ERROR message has been received from the L-2450 DAD. Close the instrument icon and try check with the maintenance software and condition change.

Parameter error

(1) Turn on power a (2) Contact the serv office.

LOG INFORMATION ERROR message has been received from the L-2450 DAD. Close the instrument icon and try check with the maintenance software and condition change.

Log information error


You're Reading a Preview EEPROM ERROR message has been Instrument information received from the L-2450 DAD. error Close the instrument icon and tryUnlock checkfull access with a free trial. with the maintenance software and condition change.

DownloadThe With Freegoes Trialout due LAMP OFF BY EXTERNAL ERROR lamp message has been received from the to external error. L-2450 DAD. Check the error condition in the Status screen and try remedial processing.


Turn on the lamp ag

LAMP OFF BY LEAKAGE message has been received from the L-2450 DAD. Check the error condition in the Status screen and try remedial processing.

Occurrence of liquid leakage.

Eliminate the cause liquid leakage, wipe leaked liquid and tu the lamp.

DSP ERROR message has been received from the L-2450 DAD. Close the instrument icon and restart the unit.

Failure in DSP operation (1) Turn onpowe the serv Sign up to vote on (2) thisContact title office.  Useful  Not useful

D2 LAMP ERROR message has been received from the L-2450 DAD.

1. The D2 lamp blows out or fails to come

1. (1) Turn on power a

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Message


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Description

W LAMP ERROR message has been received from the L-2450 DAD. Check the error condition in the Status screen and try remedial processing.



Remedy

1. The W lamp blows out or fails to come on.

1. (1) Turn on power a (2) Replace the W l with a new one. (3) Contact the serv office.

2. With both the D2 lamp and W lamp turned on, the cooling fan stops.

2., 3. If the cooling fan do not operate after tu on power again, co the service office.

3. With the W lamp alone turned on, the cooling fan stops. Hg LAMP ERROR message has been received from the L-2450 DAD. Close the instrument icon and try check with the maintenance software and condition change.

The Hg lamp goes out or fails to come on.

(1) Turn on power a (2) Replace the Hg with a new one.

SLIT ERROR message has been received from the L-2450 DAD. Close the instrument icon and restart the unit.

Failure in slit switching

(1) Turn on power (2) Contact the serv office.

SHUTTER ERROR message has been Failure in shutter received from the L-2450 DAD. operation Close the instrument icon and restart You're Reading a Preview the unit.


DAC ERROR message has been Disruption of data with ato free trial. received from the L-2450 DAD. Unlock full access transfer DAC Close the instrument icon and restart the unit.




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6.

REPLACEMENT PARTS

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Replacement Parts and Consumables

Table 6-1 lists the replacement parts and consumables u in this instrument. Their lifetime changes remarkably according to the operating condition of the instrument. The lifetime is estimated on the assumption that the instrument operates 160 hours/month (8 hour/day), 1920 hours/year (250 days). Table 6-1 Part No.

Replacement Parts and Consumables

Part Name

Estimated Lifetime

Remarks

885-3570

D 2 lamp

1000 hr/6 months

890-2527

W lamp

1000 hr/6 months

890-2237

Hg lamp unit

100 hr/2 years

890-2394

Flow cell, 10 mm

9600 hr/ 5 years

Cell alone

890-2289

Inlet tube, ID 0.25 × 0.66 T

9600 hr/5 years

Length...................... 1 m Outside diameter...... 1.57 mm

890-2288

Drain tube, 9600 hr/5 years Length...................... 2 m ID 0.33 × 0.62 TUnlock full access with a free trial. Outside diameter...... 1.57 mm




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Replacement of Lamp

Replacement of Lamp

WARNING Electric Shock due to Contact with Inside of Instrument!

Before removing the instrument cover for replacement o parts or any other work, be sure to turn off the power switch and unplug the power cord.

WARNING Never Release Safety Mechanism!

In this instrument, power is automatically cut off with the safety mechanism upon removing the lamp cover. Avoid releasing the safety mechanism intentionally to prevent electrica shock hazard which may be caused You'rean Reading Preview operational error. Unlock full access with a free trial.


CAUTION Burns due to Contact with Hot Part! •

•

The lamp and lamp cover are hot during use or immediately after use. Touching the hot lamp cove  lamp could result in burns. Sign up to vote on this title Before lamp replacement, offNot the power switch useful  Usefulturn wait for 30 minutes until the lamp cools down sufficiently.

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6.2.1 Replacement of D2 Lamp

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(1)

Items to be Prepared New D2 lamp • • Phillips screwdriver • Bladed-edged screwdriver

(2)

Check Items • Make sure that the instrument is powered off. Make sure that the power cord is unplugged from • instrument.

(3)

How to Replace D 2 Lamp

(a) Detach the flow cell cover (2 retaining screws). (b) Detach the lamp cover (2 retaining screws).

Flow cell cover

Retaining screw






Useful  Not useful Retaining screw



Lamp c

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Replacement of Lamp

(c) Loosen the three screws of the D2 lamp lead w connected to the terminal block and pull out the wires.

Termi block

Lead

(d) Remove the three lamp cover retaining screws detach the lamp cover. (Remove each lead passing it through the hole in the cover.) You're Reading a Preview Unlock full access with a free trial.


Lamp c retainin

Lamp c


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(e) Loosen the three screws retaining the D 2 lamp blade-edged screwdriver (by two turns each).

Screw

(f)

Detach the D2 lamp by turning it clockwise.




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Replacement of Lamp

(g) Fit a new D2 lamp and turn it clockwise until the no more gap between the mounting hole and retaining screw. Then tighten the three retaini screws alternately (evenly) with the blade-edge screwdriver.

D2 lamp

Retaining screw

There sho be no gap

Mounting hole

(h) Pass each lead wire through the hole in the cov Attach the cover.

You're Reading a Preview (i) Connect the lead wires to the terminal block. Unlock full access with a free trial.

(j) Put back the lamp cover and flow cell cover in Download With Free Trial (4)

Operation Check

Plug the power cord into the instrument, turn on the power switch and then make sure that the lamp is li NOTE:

If the lamp cover is attached improperly,  lamp fails to come on. Sign up to vote on this title

(5)

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Check of Lamp Energy

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(c) Click the Lamp Energy button in the Instrumen Status screen.

You're Reading a Preview (d) Make sure that the displayed lamp energy valu Unlock full access with a free trial. (250 nm) is 15,000 counts or more. Download With Free Trial


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Replacement of Lamp

(6)

Resetting of Lamp Information

(a) Click the View Maintenance button in the Instr Status screen.


(b) Click the Reset button for the D 2 lamp. Download With Free Trial


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6.2.2 Replacement of W Lamp

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(1)

Items to be Prepared New W lamp • • Phillips screwdriver • Bladed-edged screwdriver

(2)

Check Items • Make sure that the instrument is powered off. Make sure that the power cord is unplugged from • instrument.

(3)

How to Replace W Lamp


Flow cell cover

Retaining screw




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Retaining screw

Lamp cover

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Replacement of Lamp

(c) Loosen the two lamp socket retaining screws o W lamp house and pull out the lamp socket tow you. Retaining screw

You're Reading a Preview (d) Detach the W lamp from the lamp socket and a Unlock full accessW with a free trial. a new lamp. Download With Free Trial

W lam


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Operation Check

Plug the power cord into the instrument, turn on the power switch and then make sure that the W lamp i NOTE:

(5)

If the lamp cover is attached improperly, lamp fails to come on.

Check of Lamp Energy (a) Warm up the instrument for 1or 2 hours. (b) Start up EZChrom Elite for Hitachi.

(c) Click the Lamp Energy button in the Instrumen Status screen.




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Replacement of Lamp

(6)




Download Free Trial (b) Click With the Reset button for the W lamp. The ON time and ON count are reset to 0 (zero) and replacement date to the current date automatic


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6.2.3 Replacement of Hg Lamp

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(1)

Items to be Prepared New Hg lamp unit • • Phillips screwdriver

(2)

Check Items Make sure that the instrument is powered off. • • Make sure that the power cord is unplugged from instrument.

(3)

How to Replace Hg Lamp


Flow cell cover

Retaining screw



 Sign up to vote on this title Retaining screw Lamp cover  Useful  Not useful

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Replacement of Lamp

(c) Detach the Hg lamp lid (2 retaining screws).

Hg lamp lid

Retaining screw

(d) Disconnect the lead wire connector of the Hg unit from the lamp inverter. You're Reading a Preview Unlock full access with a free trial.



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(e) Loosen the setscrew of the Hg lamp unit and d it from the monochromator base.

Hg lamp unit

NOTE:

The lamp is a high-voltage part. care when handling it.



Hg lamp unit


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Attach a new Hg lamp unit to the monochromat base and tighten the setscrew.

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Replacement of Lamp

(4)

Operation Check

(a) Start up the maintenance software of EZChrom for Hitachi.

(b) Execute DAD wavelength check and in the spe display window, make sure that a sharp peak appears in the vicinity of 253.7 nm.




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Download Free Trial (b) Click With the Reset button for the Hg lamp. The ON time and ON count are reset to 0 (zero) and replacement date to the current date automatic


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APPENDIX 1. DESCRIPTION OF CONTACT SIGNAL COMMUNICATION 1.

Outline

The following two types of contact signals are used in the L-2000 series. (1)

Contact Signals Entering e-Line Connector (START, ERROR, BUSY)

These are contact signals having input/output functi that are used in common for each unit. These sign are respectively formed from the START (IN/OUT), ERROR (IN/OUT) and BUSY (IN/OUT). (2)

Individual Contact Signals Used by Each Unit

These are contact signals that are input and output You're Reading a PreviewTheir names and functions the 3-pin connector. with the unit. A name that indicates the function is Unlock full access with a free trial. at each connector.

Download With Free Trial Example of output contact signal : Example of input contact signal :

EVENT of pum LAMP OFF IN detector


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Contact Signals Entering e-Line Connector

Each unit has two e-Line connectors for connection at th of the main unit, and their signals are internally connecte By connecting each unit in series via the e-Line cable, therefore, synchronism of the L-2000 series system can easily achieved. For obtaining synchronism with the L-7000 series and/or D-2500 by way of contact signals, use the cables listed b for connection. (1)

Connection with L-7000 Series

Use 3-pin connectors of the e-DIO cable (890-6145 SDIO cable (810-7631) in the connection shown bel

START ERROR To L-2000 series instrument

STOP

To L serie instr


e-Line connector

DownloadBUSY With Free Trial 3-pin

BUSY

e-DIO cable (890-6145)

SDIO cable (890-7631)

D-line connector

connector


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Connection with Model D-2500 Use the e-DIO cable (890-6146) for connection. D-2500

H L START To L-2000 series instrument

START EXTER COM G

BUSY

H BUSY L

e-Line connector

Connect the START L and G terminals of the e-DIO (890-6146) to the EXTERNAL-COM terminal of the D-2500.




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Individual Contacts of Each Unit (1)

Contact Signal Input Terminals

These are contact signals input terminals for contro using contact signals from an external switch, relay, Any of the signals is activated by shorting the termin 0.1 second or longer. The input circuit is shown Fig. 1. (2)

Contact Signal Output Terminals

These are contact signal output terminals for contro using contact signals from an external unit. The circuit is shown in Fig. 4. The rating of contacts 12 V, 0.1 A. Make sure that the load connected not exceed this rating. e-Line cable

O


Download With Free Trial L-2000 series unit A

Fig. 1

L-2000 series unit B

Connection of e-Line Cable


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+5 V

320

Ω

IN

10 mA max. per unit OUT 4.7 k Ω ON : OFF :

(H) 0.1 A max.

5V 0V

12 V max. (reverse po prohibited)

(L) (G)

Fig. 2

Circuit Configuration of e-Line Contact


+5 V


Download With Free Trial 320

Ω

1 10 mA max.

2 3


 L-2000 series

•

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Activated upon shorting contacts of external unit.

External

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0.1 A max. 30 V max.


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1

2

3

L-2000 series

Fig. 4

External

Contact Signal Output Circuit




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APPENDIX 2. •


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TERMINOLOGY

EZChrom Elite for Hitachi Software for control of the whole HPLC system, data acquisition, data analysis and data management.

•

Slit An optical element used to let in the required part of t light.

•

Instrument parameters

Parameters for determining the operating conditions o instrument, which can be printed in list format when th D-7500 Data Processor is connected. •

Diode array detector (DAD)

You're Reading a Preview A detector for HPLC that employs a photodiode array Unlock full access with a free trial. detector. •

Download With Free Trial Download To send parameters, etc. from the upper control unit (EZChrom Elite for Hitachi for example).

•

Default Initial setting when noSign specific setting istitle input. up to vote on this

 •

D2 lamp

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