Laporan Praktikum Analisis Farmasi_ Kelompok 4_ Sulfametoksazol

LAPORAN PRAKTIKUM ANALISIS FARMASI

Analisis Kuantitatif Bahan Aktif Sulfametoksazol Dalam Sediaan Semi Solid Rabu, 30 Maret 2016 Kelompok  Rabu, Pukul 10!00 " 13!00 #I$

 Nama

NPM

L'(a Fau('))a* K!

26011013001+ 26011013001+

Tu%a& Tu%a& Pemba*a&a -a&'l, Ke&'mpula

Prat'.' Sr' A%%ra.at'

26011013001/ 26011013001/

'tor 

U%' Ra* Ra*ma ma Ku&t'a &t'a.a .a 

2601 60110130 013002 020 0

Pe Pea* a*ulu ulua, a, Ab&t Ab&tra rak k

-e%k' Sutr'&o

260110130021 260110130021

Metoe, Pemba*a&a

LA$ORATORIUM ANALISIS FARMASI FAKULT FAKULTAS FARMASI UNIRSITAS PA4A4ARAN 4ATINAN5OR  2016

Analisis Kuantitatif Bahan Aktif Sulfametoksazol dalam Sediaan Semi Solid L'(a Fau('))a* Ko&.a) 1+, Prat'.' Sr' A%%ra.at'1/, U%' Ra*ma Ku&t'a.a 20, -e%k' Sutr'&o21 Aal'&'& Farma&', Fakulta& Farma&' U'er&'ta& Pa7a7ara Prakt'kuma8ar20169%ma'l!:om

Abstrak 

Tela* 'lakuka per:obaa peetapa kaar &e)a.a &ul8ametok&a(ol alam &e'aa &alep &e:ara kuat'tat'8! Sul8ametok&a(ol merupaka at'b'ot'k %olo%a &ul8oam' )a% mem'l'k' %u%u& am' aromat'& pr'mer &e*'%%a utuk peetapa kaar)a apat 'tetapka e%a metoe 'tr'metr'! N'tr'metr' aala* metoe t'tr'metr' )a% me%%uaka atr'um 'tr't &eba%a' pet'ter alam &ua&aa a&am! Natr'um 'tr't alam &ua&aa a&am aka membetuk a&am 'tr't a aka bereak&' e%a &el! Pr'&'p 'tr'metr' '' 'a&arka paa reak&' 'a(ota&' )a'tupembetuka %aram 'a(o'um *a&'l reak&' ar' &e)a.a )a% mem'l'k' %u%u& am' aromat'& pr'mer e%a a&am 'tr't ar' atr'um 'tr't )a% beraa alam &ua&aa a&am! Paa per:obaa '' 'lakuka pembakua atr'um 'tr't me%%uaka a&am &ul8a'lat &eba%a' baku  pr'mer a ''kator )a% '%uaka aala* tropeol' oo a met'le blue ;<3 'apatka ormal'ta& ar' pet'ter )a'tu 0,0+ N &etela* 'tu, 'lakuka peetapa kaar  ar' &alep &ul8ametok&a(ol2 e%a melarutka &alep e%a pelarut a&am a&etat %la&'al 20 mL, a&am klor'a 1; mL a a'r 0 mL lalu 't'tra&' e%a laruta atr'um 'tr't e%a me%%uaka ''kator tropeol' oo a met'le blue ;<3! -a&'l )a% 'apatkaar &ul8ametok&a(ol alam &alep &ul8ametok&a(ol 2 )a'tu &ebe&ar /,2;=! Kata ku:' < &ul8ametok&a(ol, 'tr'metr', reak&' 'a(ota&','':ator  Abstract

-a& :ou:te tr'al& o8 t*e a&&a) :ompou &ul8amet*o>a(ole ' t*e o'tmet  preparat'o ?uat'tat'el)! Sul8amet*o>a(ole '& a &ul8oam'e :la&& o8 at'b'ot':& t*at *a& a pr'mar) aromat': am'e %roup& &o a& to eterm'at'o o8 leel& :a be eterm'e b)'tr'metr': t'trat'o met*o&! N'tr'metr': t'trat'o met*o& '& a t'tr'metr': met*o u&'% &o'um 'tr'te a& pet'ter uer a:'': :o't'o&! So'um 'tr'te ' a:' atmo&p*ere .'ll 8orm 'tr': a:' a .'ll rea:t .'t* t*e &le!  N'tr'metr' pr':'ple '& ba&e o t*e e&tabl'&*met o8 a 'a(ot'('% rea:t'o t*e rea:t'o o8 t*e 'a(o'um &alt :ompou *a'% a pr'mar) aromat': am'e %roup& .'t* 'trou& a:' o8 &o'um 'tr'te .ere uer a:'': :o't'o&! I t*'& e>per'met t*e &taar'(at'o o8 &o'um 'tr'te u&'% &ul8a'l': a:' a& t*e pr'mar) ra. a '':ator& u&e are tropeol' oo a met*)lee blue ;< 3 obta'e ormal't) o8 pet'ter  '& 0!0+ N a8ter 't, o t*e a&&a) o8 o'tmet &ul8ametok&a(ol2 b) '&&ol'% o'tmet .'t* a:' &olet& 20 mL %la:'al a:et':, *)ro:*lor': a:' 1; mL a 0 mL o8 .ater  a t*e t'trate .'t* &o'um 'tr'te u&'% '':ator& tropeol' oo a met*)lee blue ;< 3! T*e re&ult& obta'e ' t*e o'tmet &ul8amet*o>a(ole &ul8amet*o>a(ole leel& 2 t*at '& e?ual to /!2;=! Ke).or&< &ul8amet*o>a(ole, 'tr'metr', 'a(ot'('% rea:t'o, t*e '':ator 

[ Analisis Kuantitatif Bahan Aktif Sulfametoksazol ]

Rabu, 30 Maret 2016

e%a me%%uaka atr'um 'tr't

Pendahuluan

Sul8ametok&a(ol

aala*

obat

)a% 'a&amka, me)ebabka 8u%&'

%olo%a &ul8oam' )a% mempu)a'

am'* aromat'k pr'mer 'uba* me7a'

&pektrum at'bakter' )a% lua&! Obat

%aram 'a(o'um, &epert' paa reak&'

'' merupaka ata%o'& kompet't'8  &ul8a&etam'a e%a a&am 'tr't! ; PA$A

&e*'%%a

 pembetuka

a&am

me%*ambat 8olat

)a%

Metoe t'tra&' 'a(ota&' '&ebut  7u%a 'tr'metr' )a'tu metoe peetapa

'%uaka ole* bakter' utuk &'te&'&

kaar

&e:ara

kuat'tat'8

e%a

 pur' a a&am ukleat!2

me%%uaka laruta baku NaNO₂! Metoe '' 'a&arka paa reak&'

Struktur &ul8ametok&a(ol

'a(ota&' )ak' reak&' atara am'a aromat'k pr'mer e%a a&am 'tr't alam

&ua&aa

a&am

membetuk 

%aram! T't'k ak*'r t'tra&' 'a(ota&' ter:apa'

apab'la

paa

pe%%ore&a

laruta )a% 't'tra&' paa pa&ta ka7' Rumu& &ul8ametok&a(ol mem'l'k'

$erat

molekul

ar'

'o'a atau kerta& ka7' 'o'a aka

@10-11 N3O3S

a

terbetuk .ara b'ru 7u%a!

molekul

3

2;3,2+!

Sua* k'ta l'*at ba*.a alaam

Pemer'a &erbuk *ablur, put'* &a'

t'tra&' reok& aa ua 7e'& ''kator,

*amp'r put'*, prakt'& t'ak berbau!

''kator )a% k*u&u& bereak&' e%a

Kelaruta)a prakt'& t'ak larut alam

&ala* &atu kompoe )a% bereak&' a

a'r, alam eter a alam kloro8orm,

''kator ok&'a&' reuk&' )a% t'ak 

mua* larut alam a&eto a alam

ter%atu% ar' &ala* &atu at tetap'

laruta atr'um *'rok&'a e:er, a%ak 

*a)a paa pote&'a laruta &elama

&ukar larut alam etaol!  

t'tra&'! 6

T'tra&' 'a(ota&' aala* t'tra&' )a%

alam t'tra&' 'a(ota&', '%uaka

:ukup &eer*aa utuk 'lakuka a

ua ma:am ''kator, )a'tu ''kator 

&a%at ber%ua utuk aal'&'& at'b'ot'k 

alam a ''kator luar! Seba%a'

&ul8oam'a a aa&tat'k lokal turu

''kator alam '%uaka :ampura

a&am be(oat! T'tra&' '' 'lakuka

''kator tropeol' oo a met'le b'ru,

3

[ Analisis Kuantitatif Bahan Aktif Sulfametoksazol ]

Rabu, 30 Maret 2016

'%'ka

u%u

ke*'7aua!

Tamba*ka K$r &eba)ak 10 m% !

Sea%ka utuk ''kator luar)a

T'tra&' e%a laruta NaNO2 0,1 M

me7a'

b'ru

'%uaka kerta& ka7' 'o'e!

6

&a'

1; o@!

)a% me%alam' peruba*a .ara ar'

&u*u

*'%%a ter7a' peruba*a .ara laruta u%u me7a' b'ru ke*'7aua

Metode Penelitian

Pembuatan ndikator !ro"eolin ##

T'mba%

Alat

Alat

)a%

'%uaka

&ek&ama

1;0

m%

paa

Tropeol' OO! Larutka e%a 100 ml

 per:obaa '' aala* buret, erleme)er,

a?uae&t alam beaker %la&&! Paa&ka

olume p'pet, %ela& ukur, beaker %la&&,

laruta &amb'l 'auk perla*a *'%%a

 p'pet tete&, mort'r &tamper, lau ukur,

larut

a t'mba%a

Pembuatan ndikator metilen blue

T'mba% &ek&ama 100 m% &erbuk 

Bahan

$a*a

)a%

paa

met'le blue! Larutka e%a 100 mL

 per:obaa '' aala* NaNO2, A&am

a?uae&t alam beaker %la&&! Paa&ka

&ul8a'lat,

laruta paa &u*u 60B0o@ &amb'l

-NO3,

'%uaka

&ul8ametok&a(ol,

Met'le blue, Trpeol' oo!

'auk perla*a *'%%a larut!

Prosedur Penelitian

Peneta"an Kadar

Pembuatan Natrium Nitrit 0,1 N

Sulfametoksazol dalam sam"el sale"

Sampel

dalam 1 L

T'mba% e%a &ek&ama ,3 %ram

'*omo%eka

&alep

)a%

't'mba%

tela* ma&'%B

 NaNO2! Larutka e%a a?uae&t a

ma&'% 0,2;/1 %ram a 0,2;63 %ram,

1000 mL!

larutka alam :ampura 20 mL a&am

Pembakuan Natrium Nitrit

a&etat %la&'al P, 0 mL a'r a 1; mL

T'mba% 100 m% a&am &ul8a'lat

a&am

klor'a

P

paa&ka

alam

e%a &ek&ama! Larutka alam labu

 pea%a& a'r &amb'l &e&ekal' '%o)a%B

rleme)er e%a 2; mL a?uae&t!

%o)a% utuk memua*ka pem'&a*a

Tamba*ka -@I N &eba)ak ; mL!

a&el', &etela* (at akt'8 larut, laruta

Ma&ukka ; tete& tropeol' oo 0,1=

 b'arka '%' paa &u*u kamar a

a

aka terl'*at a&el' memaat paa

3

tete&

met'le

blue

0,1=!

4

[ Analisis Kuantitatif Bahan Aktif Sulfametoksazol ]

 permukaa laruta! Ma&ukka ; tete& tropeol' 00 0,1= a 3 tete& met'le  blue 0,1=! '%'ka &a' &u*u

Rabu, 30 Maret 2016

Sam"el Sulfametoksazol %

1E 1,3 ml

2 E 1,1 ml

m&elE 2;/,1 m%

m&elE 2;6,3 m%  N1 1 E

o

1; @ Ct'ak perlu ':elup termometerD, amu

7'ka perlu lakuka blako

laruta a'r )a% ''%'ka paa

 N1 1E 1,3 > 0,0+ E

.aa* )a% berbea amu e%a .aktu pe'%'a )a% &ama e%a laruta

&el!

Tamba*ka

1,1 > 0,0+ E

K$r 

&eba)ak 10 m%! T'tra&' e%a laruta  NaNO2 0,1 M *'%%a ter7a' peruba*a .ara

laruta

u%u me7a'

mmur'E 26,3 m%

b'ru

ke*'7aua

mmur'

$asil

m% =Kaar 2 E

=Kaar 1 E

E 22,2+

Pembakuan NaN#%

> 100=

Sam"el Sulfametoksazol %

1E 6, ml

2E 6,6 ml

 N1> 1 E N2> 2

 N1> 1 E N2> 2

 6,! N2

 6,6!

=Kaar 1 E

> 100=

>

100=

 6,! N2

>

100= E

 N2

=Kaar 2 E

E +,6/=

10,16=  6,6!

 N2E 0,0+1 N

=Kaar rata&rata E

' /,2;=

 N2  N2E 0,0/ N

Nrata&rata  '

' 0,0+ N

Pembahasan

T'tra&' 'tr'metr' '%uaka utuk  Penetapan Kadar Sulfametoksazol 2

meetuka

kaar

&e)a.a

)a%

mem'l'k' %u%u& am' pr'mer! Am'

5

[ Analisis Kuantitatif Bahan Aktif Sulfametoksazol ]

 pr'mer

apat

%aram

'a(o'um,

e%a

la%&u% membetuk 

'o

ket'ka

'tro&o'um

bereak&' karea

Rabu, 30 Maret 2016

reak&'! K$r aka ber'kata e%a NO 2 membetuk

'tro&obrom'

)a%

kemu'a membetuk 8eol! Sela' 'tu

mem'l'k' ua proto C-D &ea%ka  7u%a '%uaka &eba%a' &tab'l'&ator  am'

&ekuer

perlu

'*'rol'&'&

a%ar a&am 'tr't )a% terbetuk t'ak 

terleb'* a*ulu me7a' am' pr'mer  terura'! T'tra&' e%a laruta NaNO2 karea *a)a mem'l'k' &atu -  e%a

*'%%a ter7a' peruba*a .ara laruta

em'k'a pembetuka *a)a &a'

u%u

terbetuk 'o 'tro&am'! Sea%ka

'perole* rataBrata ormal'ta& laruta

am' ter&'er t'ak apat 'tetuka

 NaNO2 ar' pembakua I C0,0+1 ND

me7a'

b'ru

ke*'7aua!

me%%uaka 'tr'metr' karea t'ak  a pembakua II C0,0/ ND &ebe&ar  mem'l'k' - ak'bat)a prouk )a% terbetuk kura% &tab'l Pet'ter

paa

t'tra&'

0,0+ N! Setela*

'tr'metr'  NaNO2

melakuka

'lakuka

pembakua

peetapa

kaar 

aala* NaNO2 )a% merupaka laruta

&el &ul8ametok&a(ol 2! $era&arka

 baku

rumu&

&ekuer!

Maka

&ebelum

&truktur)a, apat

'keta*u'

'lakuka t'tra&' utuk peetua kaar   ba*.a &ul8ametok&a(ol mem'l'k' %u%u& &el, 'lakuka pembakua terleb'*

am' aromat'k pr'mer, &e*'%%a alam

a*ulu utuk me%eta*u' ko&etra&'  peetapa kaar)a apat '%uaka  pet'ter! Pembakua laruta NaNO2

metoe t'tra&' 'tr'metr'!3 Sampel &alep

me%%uaka a&am &ul8a'lat )a%

&ul8ametok&a(ol

tela* 'tamba*ka a?uae&t a a&am

ma&'% &alep 0,2;/1 %ram a 0,2;63

klor'a )a% kemu'a 't'tra&' e%a

%ram )a% kemu'a 'tamba*ka

laruta NaNO2! Pembakua 'lakuka

e%a a&am a&etat %la&'al, a'r, a

uplo, ma&'%Bma&'% a&am &ul8a'lat

a&am

 berat)a aala* 100 m%! 'ma&ukka ;

'lakuka

tete& tropeol' 00 0,1= a 3 tete&

me7a' a&am! alam ko'&' a&am,

met'le blue 0,1= a ''%'ka

aka

&a' &u*u 1;o@! Lalu meamba*ka

'a(o'um )a% bera&al ar' reak&'

K$r &eba)ak 10 m% ! peamba*a

atara am' aromat'k

K$r bermak&u utuk memper:epat

&ul8ametok&a(ol e%a t'tra atr'um

't'mba%

klor'a!

Peamba*a

utuk

ter7a'

ma&'%B

membuat

pembetuka

-@l

&ua&aa

%aram

pr'mer ar'

6

[ Analisis Kuantitatif Bahan Aktif Sulfametoksazol ]

'tr't!

3

-@l )a% 'tamba*ka aka

Rabu, 30 Maret 2016

laruta ''%'ka &a' &u*u 1; o@!

 bereak&' e%a NaNO2 maka aka

Su*u

terbetuk A&am N'tr't membetuk 'o

 pro&eur t'tra&' 'tr'metr'! T'tra&' ''

'tro&o'um )a% kemu'a terbetuk 

&eba'k)a 'lakuka paa &u*u )a%

%aram 'a(o'um )a% aka bereak&'

rea* C'%'D! Reak&' atara am'

e%a am' pr'mer! Pet'ter )a%

aromat'k pr'mer e%a a&am 'tr't

'%uaka

 paa

t'ak

la%&u%

&a%at

&u*u

berpe%aru*

)a%

'%'

alam

aka

me%%uaka A&am N'tr't karea &'8at

membetuk %aram 'a(o'um )a%

A&am N'tr't )a% mua* me%uap

t'ak &tab'l!6,1  Sela' 'tu

&e*'%%a t'ak &tab'l alam pro&e&

a.al reak&', )ak' pembetuka a&am

t'tra&'

'

'tr't, a&am 'tr't :eeru% t'ak &tab'l

 pea%a& a'r! Saat pemaa&a, &alep

a mua* terura'! 6 'tamba*ka K$r 

ter:ampur

&eba)ak 10 m% kemu'a 't''tra&'

Kemu'a

a

'paa&ka

pemba.a

beraa

'lap'&a ata& ket'ka ''%'ka! ;

e%a

laruta

paa ta*ap

NaNO2 

*a&'l

tete& tropeol' 00 0,1= a 3 tete&  pembakua C0,0+ ND *'%%a ter7a' met'le blue 0,1= 'ma&ukka ke  peruba*a .ara laruta u%u me7a' laruta &el )a% &ua* 'p'&a*ka

 b'ru ke*'7aua!

ar' pemba.a)a! Keua ''kator ''

 NaNO2 )a% 'perluka utuk t'tra&'

aala* ''kator alam, Tropeol' OO

&eba)ak 1,3 ml a 1,1 ml! ar'

merupaka ''kator a&am ba&a )a%

olume ter&ebut kemu'a 'perole*

 ber.ara mera* 7'ka beraa alam

kaar &alep rataBrata ar' peetapa I

&ua&aa

C10,16 =D a peetapa II C+,6/ =D

a&am a

aka ber.ara

ku'% b'la 'ok&'a&' ole* aa)a kelab'*a met'le

a&am b'ru

 pe%kotra&

'tr't,

aala* &ebe&ar /,2; =!

&ea%ka

'%uaka .ara!

'apatka olume

&eba%a'

Pe%%uaa

Sim"ulan

Prepara&' &alep

&el

paa

&el

keua ''kator '' &e:ara ber&amaa

&e'aa

&u8ametok&a(ol

2

me%a*a&'lka .ara

laruta )a%

'lakuka e%a pem'&a*a (at akt'8 

 ber.ara u%u a paa t't'k ak*'r 

a ba&'& ar' &alep melalu' :ara

t'tra&' ter7a' peruba*a .ara me7a'

 pemaa&a a pe'%'a paa &u*u

*'7au to&:a! Setela* 'tamba* ''kator,

kamar! Metoe aal'&'& )a% '%uaka

7

[ Analisis Kuantitatif Bahan Aktif Sulfametoksazol ]

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8