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UNIVERSIDAD NACIONAL DEL FACULTAD DE INGENIERÍA AMBIENTAL Y RECURSOS “Año de la consolidación del Mar de Grau”
COLORACIÓN DIFERENCIAL INFORME DE LABORATORIO N°3 y 4
INFORME DE LABORATORIO 93G ALUMNO: SANCHEZ CARHUAPOMA, LUIS ANGEL PROFESORA:
OBJETIVO
Objetivo Expei!e"t#$ N°% • •
Ob&ev# $#& 'i(#& )e$ *o"go+ Ape")e # pep## !,e&t#& )e *o"go&
Objetivo Expei!e"t#$ N°• •
• •
.&o )e !/& )e ," 0o$o#"te e" ," po0e&o )e 0o$o#0i1"+ Re0o"o0e $o& !i0oog#"i&!o& 2#! po&itivo& y "eg#tivo& )e $# !,e&t# b,0#$+ Co"o0e $#& t0"i0#& )e 0o$o#0i1" )i(ee"0i#$ b#0tei#"#+ .&o 0oe0to )e $#& t0"i0#& )e 0o$o#0i1" )i(ee"0i#$+
INTROD.CCIÓN
M,0*o& )e $o& !i0oog#"i&!o& 5,e e&xi&te" &o" )i(ee"te& )e !#"e# (6&i0# y 5,6!i0# 0o!o # &, ve7 e&t#& )i(ee"0i#& pe&e"te& e"te e$$o& &e )ebe" # $#& )i&ti"t#& (o!#" e" $# 5,e e#00io"#" #"te )ete!i"#)o& po0e&o& )e 0o$o#0i1" 0o!o $o& 5,e !e"0io"#e!o& !/& #)e$#"te po e$$o e" e&t# p#0ti0#)e $#bo#toio #pe")ee!o& )e $#& v#i#& t0"i0#& )e 0o$o#0i1" 5,e pe!it#" )i(ee"0i# g,po& )e !i0oog#"i&!o& )e oto&+ MARCO TEORICO
Técnicas de tinción. Fundamentos Lo& (,")#!e"to& )e $# t0"i0# &e b#&#" e" $#& )i(ee"0i#& e"te $#& p#e)e& 0e$,$#e& )e $#& b#0tei#& g#! po&itiv#& y g#! "eg#tiv#& L# p#e) 0e$,$# )e $#& b#0tei#& g#! po&itiv#& po&ee ,"# g,e&# 0#p# )e pepti)og$i0#"o #)e!/& )e )o& 0$#&e& )e /0i)o& tei0oi0o&8 #"0$#)o e" $# 0## i"te"# )e $# p#e) 0e$,$# y ,"i)o # $# !e!b#"# p$#&!/ti0# &e e"0,e"t# e$ /0i)o $ipotei0oi0o y !/& e" $# &,pe(i0ie e$ /0i)o tei0oi0o 5,e e&t/ #"0$#)o &o$#!e"te e" e$ pepti)og$i0#"o 9t#!bi" 0o"o0i)o 0o!o !,e6"#:+
;o e$ 0o"t#io $# 0#p# )e pepti)og$,0#"o )e $#& b#0tei#& g#! "eg#tiv#& e& )e$g#)# y &e e"0,e"t# ,"i)# # ,"# &eg,")# !e!b#"# p$#&!/ti0# exteio 9)e 0o!po&i0i1" )i&ti"t# # $# i"te"#: po !e)io )e $ipopote6"#&+ Tie"e ,"# 0#p# )e$g#)# )e pepti)og$i0#"o ,"i)# # ,"# !e!b#"# exteio po $ipopote6"#&+ L# !e!b#"# exteio e&t/ *e0*# )e pote6"# (o&(o$6pi)o y $ipopo$i�/i)o+ ;o $o t#"to #!bo& tipo& )e b#0tei#& &e ti
vio$/0e#+ ;o e$ 0o"t#io $#& b#0tei#& g#! po&itiv#& #$ po&ee ,"# p#e) 0e$,$# !/& e&i&te"te y 0o" !#yo popo0i1" )e pepti)og$i0#"o& "o &o" &,&0eptib$e& # $# #00i1" )e$ &o$ve"te og/"i0o &i"o 5,e e&te #0t?# )e&*i)#t#")o $o& poo& 0e/")o$o& $o 5,e i!pi)e 5,e p,e)# e&0#p#&e e$ 0o!p$ejo 0i&t#$ vio$et#=yo)o y !#"te"ie")o $# 0o$o#0i1" #7,$>vio$et#+ Lo& !to)o& )e ti"0i1" &o" )e g#" ,ti$i)#) peo )ebe" ,&#&e &ie!pe 0o" pe0#,0i1" y# 5,e p,e)e" 0o"),0i # eoe&+ L#& !o$0,$#& )e 0o$o#"te (o!#" e" o0#&io"e& pe0ipit#)o& o #geg#)o& 5,e p#e0e" e&t,0t,#& 0e$,$#e& #,t"ti0#& peo 5,e &o" (o!#0io"e& 0o!p$et#!e"te #ti(i0i#$e& i"),0i)#& po e$ !i&!o 0o$o#"te+ T#$e& e&t,0t,#& &e )e"o!i"#" #te(#0to& y )ebe" to!#&e !,0*#& pe0#,0io"e& p## te"e $# &eg,i)#) )e 5,e "o "o& e&t#!o& e5,ivo0#")o #$ 0ee 5,e ," #te(#0to e& ,"# e&t,0t,# e#$!e"te exi&te"te+
Factores que alteran la tinción Gram A pe&# )e $# g#" ,ti$i)#) )e $# ti"0i1" )e 2#! e&te !to)o )ebe &e v#$o#)o 0o" pe0#,0i1" y# 5,e $# e#00i1" p,e)e v#i# &eg?" $# e)#) )e $#& 0$,$#& 90,$tivo& viejo& )e b#0tei#& g#! po&itiv#& p,e)e" pe)e 0#p#& )e pepti)og$i0#"o& y te
p,e)e 0oe e$ ie&go 5,e b#0tei#& g#! po&itiv#& &e ti<#" 0o!o g#! "eg#tiv#&:+ ;o e&t# &it,#0i1" j,"to # $# !,e&t# )ebe" te
E)#) )e $# b#0tei#+ Eoe& )e$ ope#)o+ .&o )e #"tibi1ti0o&
TINCIÓN GRAM L# ti"0i1")e g#! o 0o$o#0i1" )e g#! e& ," tipo )e ti"0i1" )i(ee"0i#$ e!p$e#)o e" !i0obio$og6# p## $# vi&,#$i7#0i1" )e b#0te6#& &obe to)o e" !,e&t#& 0$6"i0#&+ Debe &, "o!be #$ b#0tei1$ogo )#"& C*i&ti#" 2#! 5,e )e&#o$$1 $# t0"i0# e" %4+ &e ,ti$i7# t#"to p## po)e e(ei&e # $# !o(o$og6# 0e$,$# b#0tei#"# 0o!o p## po)e e#$i7# ,"# pi!e# #poxi!#0i1" # $# )i(ee"0i#0i1" b#0tei#"# 0o"&i)e/")o&e g#! po&itiv# # $#& b#0tei#& 5,e &e vi&,#$i7#" )e 0o$o vio$et# y b#0tei# g#! "eg#tiv# # $#& 5,e &e vi&,#$i7#" )e 0o$o o&#+ T0"i0# • • • • • • • •
;ep## e$ (oti& y (ij#$o #$ 0#$o Co$o0# $# pep##0i1" (ij#)# 0o" $# &o$,0i1" )e 0i&t#$ vio$et# ),#"te $ L#v# 0o" &o$,0i1" yo)#)# 9L,go$ : C,bi e$ (oti& 0o" L,go$ ),#"te % E&0,i y )e0o$o# po #$0o*o$ *#&t# 5,e "o ##&te !/& 0i&t#$ vio$et#+ L#v# 0o" #g,# Co"t#&t# 0o" $# &o$,0i1" )e (,0&i"# po % L#v# 0o" #g,# &e0# #$ #ie y ex#!i"# 0o" objetivo )e i"!e&i1"
B#0tei#& 2#! ;o&itiv#& E" !i0obio$og6# &e )e"o!i"#" b#0tei#& 2#! po&itiv#& # #5,e$$#& b#0tei#& 5,e &e ti po&itiv#& o t#!bi" g#!po&itiv#&+ E&t# 0##0te6&ti0# e&t/ 6"ti!#!e"te $ig#)# # $# e&t,0t,# )e $# e"vo$t,# 0e$,$# po $o 5,e e($ej# ," tipo "#t,#$ )e og#"i7#0i1" b#0tei#"#+ @o" ,"o )e $o& pi"0ip#$e& g,po& )e b#0tei#& y 0,#")o &e t#t#" 0o!o t#x1" &e ,ti$i7# t#!bi" e$ "o!be )e ;o&ib#0tei#+
L# e"vo$t,# 0e$,$# )e $#& b#0tei#& 2#!>po&itiv#& 0o!pe")e $# !e!b#"# 0itop$#&!/ti0# y ,"# p#e) 0e$,$# 0o!p,e&t# po ,"# g,e&# 0#p# )e pepti)og$,0#"o 5,e o)e# # $# #"teio+ L# p#e) 0e$,$# &e ,"e # $# !e!b#"# 0itop$#&!/ti0# !e)i#"te !o$0,$#& )e /0i)o $ipotei0oi0o+ L# 0#p# )e pepti)og$,0#"o 0o"(iee ,"# g#" e&i&te"0i# # e&t#& b#0tei#& y e& $# e&po"&#b$e )e ete"e e$ ti"te ),#"te $# ti"0i1" )e 2#! + I"0$,ye" e&pe0ie& t#"to !1vi$e& 9v6# ($#ge$o&: 0o!o i"!1vi$e& 0o" (o!# )e b#0i$o 9B#0i$$,&
C$o&ti)i,!
Coy"eb#0tei,!
L#0tob#0i$$,&
Li&tei#:
o
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9@t#p*y$o0o00,& @tepto0o00,&: 0o" g,e&#& p#e)e& 0e$,$#e& o &i" e$$#& 9My0op$#&!#:+ A$g,"#& e&pe0ie& &o" (oto&i"tti0#& peo $# !#yo6# &o" *ete1to(#&+ M,0*#& )e e&t#& b#0tei#& (o!#" e")o&po#& e" 0o")i0io"e& )e&(#vo#b$e&+ Re#$!e"te "o to)#& $#& b#0tei#& )e$ g,po &o" 2#! po&itiv#& 9"o &e ti po&itiv#&+ B#0tei#& C$o&ti)i,! pe(i"ge"& 92#! po&itiv#&:
B#0tei#& 2#! Neg#tiv#&
L#& B#0tei#& 2#!>Neg#tiv#& &o" #5,e$$#& 5,e pe&e"t#" )o& !e!b#"#& $ip6)i0#& y e"te e$$#& &e $o0#$i7# ," (i"# p#e) 0e$,$# 0o!p,e&t# (,")#!e"t#$!e"te po pepti)og$,0#"o 9 #$ &e (i"# e&t# p#e) "o &e ti"eg#tiv#& 0#,&#" e"(e!e)#)e&+ Lo& 0o0o& 2#!> "eg#tivo&
0#,&#" $# go"oe# 9Nei&&ei# go"o*oe#e: !e"i"giti&9Nei&&ei#
!e"i"giti)i&: y &6"to!#& e&pi#toio& 9Mo#xe$$# 0#t#*#$i&: e"te oto&+ Lo& b#0i$o& 2#!>"eg#tivo& i"0$,ye" ," g#" "?!eo )e e&pe0ie&+ A$g,"o& )e e$$o& 0#,&#" pi"0ip#$!e"te e"(e!e)#)e& e&pi#toi#& 9'#e!op*i$,& i"($,e"7#e $eb&ie$$# p"e,!o"i#e Legio"e$$# p"e,!op*i$# ;&e,)o!o"#& #e,gi"o&#: e"(e!e)#)e& ,i"#i#& 9E&0*ei0*i# 0o$i ;ote,& !i#bi$i& E"teob#0te 0$o#0#e @e#ti# !#0e&0e"&: y e"(e!e)#)e& g#&toi"te&ti"#$e& 9'e$i0ob#0te py$oi @#$!o"e$$# e"teiti)i& @#$!o"e$$# typ*i:+ Oto& e&t/" #&o0i#)#& # i"(e00io"e& "o&o0o!i#$e& 9A0i"etob#0te b#,!#"ii:+ B#0tei#& E&0*ei0*i# 0o$i 92#! "eg#tiv#&: vi&t#& #$ !i0o&0opio t#& &e te
TINCIÓN WIRTZ – CN!"IN
Lo& e#0tivo& pe&e"te& &o" &o$,0i1" #0,o&# )e ve)e !#$#5,it# G y &o$,0i1" #0,o&# )e &#(#"i"# #$ HG+ @e ob&ev#" 0o" $e"te )e i"!e&i1" $#& e&po#& &e 0o$oe#" )e ve)e y $#& b#0tei#& )e 0o$o ojo i"te"&o+
R#AMINA$A%RAMINA Lo& /0i)o& !i01$i0o& )e $#& p#e)e& 0e$,$#e& )e $#& !i0ob#0tei#& po&ee" #(i"i)#) p## $o& ($,oo0o!o& #,#!i"# y o)#!i"#+ E&to& 0o$o#"te& &e (ij#" # $#& b#0tei#& 5,e #p#e0e" )e 0o$o #!#i$$o o "##"j# bi$$#"te 0o"t# ," (o")o ve)o&o+ E$ pe!#"g#"#to )e pot#&io e!p$e#)o 0o!o 0o"t#&te evit# $# ($,oe&0e"0i# i"e&pe06(i0#+ To)o& $o& !i0oog#"i&!o& /0i)o>#$0o*o$ e&i&te"te& i"0$,ye")o $o& e&poo7o#io& p#/&ito& &e ti#,#!ii# e& 5,e $,ego $o& (oti& p,e)e" &e eteNee$&e" o i"yo," )ie0t#!e"te &obe $# ti"0i1" 0o" e$ ($,oo0o!o &i &e e$i!i"# #"te& e$ #0eite )e i"!e&i1"+ De e&t# (o!# $o& e&,$t#)o& po&itivo& p,e)e" &e 0o"(i!#)o& 0o" $#& 0o$o#0io"e& t#)i0io"#$e& 5,e #)e!/& pe!ite" $# )i(ee"0i#0i1" !o(o$1gi0#+
NARAN&A #' ACRI#INA E$ ($,oo0o!o "##"j# )e #0i)i"# &e ,"e #$ /0i)o ",0$ei0o y# &e# e" &, (o!# "#tiv# o )e&"#t,#$i7#)#+ E" #$g,"#& pep##0io"e& )e "##"j# )e #0i)i"# e$ 0o$o )e $# ($,oe&0e"0i# p,e)e v#i# )epe")ie")o )e$ p' y )e $# 0o"0e"t#0i1"+ E$ "##"j# )e #0i)i"# *# &i)o e!p$e#)o 0o!o 0o$o#"te vit#$ 5,e )# ,"# ($,oe&0e"0i# ve)e &i e$ !i0oog#"i&!o e&t/ vivo y oj# &i e&t/ !,eto+ De to)o& !o)o& 0o!o e$ 0o$o#"te &e i"te0#$# e" e$ /0i)o ",0$ei0o e$ ge!e" vi#b$e &e i"#0tiv#/ po0o tie!po )e&p,& )e $# ti"0i1"+ E$ ,&o )e "##"j# )e #0i)i"# p## )ete0t# $# pe&e"0i# )e b#0tei#& e" $o& *e!o0,$tivo& *# &i)o #!p$i#!e"te #0ept#)o+ De *e0*o ,"# g#" 0#"ti)#) )e e&t,)io& *#" )e!o&t#)o 5,e $# ti"0i1" )e *e!o0,$tivo& 0o" "##"j# )e #0i)i"# e& t#" &e"&ib$e 0o!o e$ &,b0,$tivo 0iego p## $# )ete00i1" i"i0i#$ )e *e!o0,$tivo& po&itivo&+
TINCIN #' ZI'(" N''")'N
L# ti"0i1" )e ie*$>Nee$&e" 9BAAR: e& ,"# t0"i0# )e ti"0i1" )i(ee"0i#$ /pi)# y e0o"1!i0# p## $# i)e"ti(i0#0i1" )e !i0oog#"i&!o& p#t1ge"o& po eje!p$o M+ t,be0,$o&i&+ F,e )e&0it# po pi!e# ve7 po )o& !)i0o& #$e!#"e& F#"7 ie*$ 9% # %-K: ," b#0tei1$ogo y Fie)i0* Nee$&e" 9%4 >%4: ," p#t1$ogo+ L#& p#e)e& 0e$,$#e& )e 0ieto& p#/&ito& y b#0tei#&+ Co"tie"e" /0i)o& g#&o& 9/0i)o& !i01$i0o&: )e 0#)e"# $#g# 9H # H /to!o& )e 0#bo"o: 5,e $e& 0o"(iee" $# popie)#) )e e&i&ti $# )e0o$o#0i1" 0o" #$0o*o$>/0i)o )e&p,& )e $# ti"0i1" 0o" 0o$o#"te& b/&i0o&+ ;o e&to &e )e"o!i"#" /0i)o>#$0o*o$ e&i&te"te+ L#& !i0o b#0tei#& 0o!o M+ t,be0,$o&i& y M+ !#i",! y $o& p#/&ito& 0o006)eo& 0o!o Cypto&poi)i,! &e 0##0tei7#" po &,& popie)#)e& )e /0i)o>#$0o*o$ e&i&te"0i#+ L# 0o$o#0i1" 0$/&i0# )e ie*$ Nee$&e" e5,iee 0#$e"t#!ie"to p## 5,e e$ 0o$o#"te #t#vie&e $# p#e) b#0tei#"# 5,e 0o"tie"e 0e#&+ @e *# )e&#o$$#)o ,"# 0o$o#0i1" )e /0i)o>#$0o*o$ e&i&te"0i# !o)i(i0#)# 5,e )i(ee"0i# $#& e&pe0ie& )e No0#)i# 9b#0tei#& #!i(i0#)#& (i$#!e"to&#& 0,y#& p#e)e& 0e$,$#e& 0o"tie"e" /0i)o&>g#&o& )e ,"o& H /to!o& )e 0#bo"o: )e $o& #0ti"o!i0eo& 9!,y &e!ej#"te& peo "o /0i)o>#$0o*o$ e&i&te"te&:+ No0#)i# &pp &o" )e0o$o#)#& po $# !e70$# /0i)o>#$0o*o$ e&t/")# peo "o po ," t#t#!ie"to !/& &,#ve 0o" /0i)o &,$(?i0o H # %G+ E&to& !i0oog#"i&!o& &e )e"o!i"#" /0i)o>#$0o*o$ e&i&te"te& p#0i#$e& o )bi$e&+ E$ (oti& &e tiKH &eg+ Co" A7,$ )e Meti$e"o 90o$o )e 0o"t#&te:+ L#v# y &e0# TECNICA8
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C,bi $# $/!i"# pevi# (ij#0i1" )e $# pep##0i1" po 0#$o 0o" $# (,0&i"# (e"i0#)# )e ie*$ Nee$&e" y 0#$e"t#$# *#&t# 5,e e!it# v#poe& &i" 5,e *iev# ),#"te te& # 0i"0o !i",to& e&po")ie")o 0o"&t#"te!e"te e$ 0o$o#"te p##
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evit# $# )e&e0#0i1"+ L#v# 0o" #g,# 0oie"te+
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COM;O@ICION8
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MATERIALE@
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M#tei#$e& Bio$og60o&+ B#te6# p## ti"0i1" )e 2#!+
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B#te6# p## ie*$ Nee$&e"+ Mi0o0opio+ ;ot#objeto&+ C,be objeto&+ A&# )e &ie!b#+ Me0*eo+ ;i"7# )e !#)e#+
;ROCEDIMIENTO
Expeie"0i# 0o" $# !,e&t# )e *o"go8 • • • • •
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Expeie"0i# 0o" $# !,e&t# )e &#o b,0#$8 •
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M,e&t# N°%
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