LAPORAN PRAKTIKUM “ IDENTIFIKASI IDENTIFIKAS I BAKTERI BERBAHAY BERBA HAYA A PADA JAJANAN MINUMAN MINUM AN “
OLEH :
NAMA
: KADEK INTAN ARNI S
NIM
: D1C1 14 050
KELOMPOK KELOMPO K
: IV (EMPAT) (EMPAT)
KELAS
: TPG_A 2014
JURUSAN ILMU DAN TEKNOLOGI PANGAN FAKULTAS TEKNOLOGI DAN INDUSTRI PERTANIAN UNIVERSITAS HALU OLEO KENDARI 2016
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I.
PENDAHULUAN
I.1. Latar Belakang Belakang
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!-/ .,+-" Escherichia .,+-" Escherichia coli $. $. '-"*- '-"*-!" !" $/-3 $/-3 !,%-/ !,%-/33 $" .-"*! .-"*! %/" .-"-/"" %""* 9!-/ Escherichia 9!-/ Escherichia coli (E.Coli) '-/.! '-/.!" " %& %& !-/ !-/ *"+ *"+ .%"+ .%"+ "*! -/$. .$ / "+ K,"-"/ E.Coli K,"-"/ E.Coli $%' / "+ .-""+ P/,- .-"+"!7" !-/ E.Coli !-/ E.Coli -/ !' -.-/: !%,/"3 ,>," '-'-/!" -7-! *"+ !/"+ !132 S-&"++ .-/% $"* %-/"7 -/&$. '%& -/- -/- S"/ U%/8,%- U%/8,%- '-/.!" '-/.!" "/ *"+ $+"!" "! '-'"& !-/ -/' .$ / * E.Coli * E.Coli U%/8,%- *"+ -/%-! $"/ ?R* $" 8%8%- %+& '-'%! '-'%! !-''." !-''." -+ -+ $-"7-!" $-"7-!" -/ 7! 7! %& "* $%' .-"+,%&" / '"'
I.. T! T!"!an "!an
T# T#" " $/ $/ ./! ./!!' !' *"+ *"+ -%& -%& $%!! $%!!" " $%& $%& "! "! '-"+$ '-"+$-" -"7 7! ! !-/ -/&* *"+ $ .$ ##"" '"'"
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II.
TINJAUAN PUSTAKA
M"'" ##"" '-/.!" %& !-&" ."+" *"+ $&!" "!" "!"! ! -/$ -/$ $%/ $%/ /'& /'& '%"* '%"* $ !"" !"" -!,% -!,%& & K% K% $/ $/ '"'" *"+ %*! "! $!,"' "!"! .$ $/"* $.-"+/& ,%-& '!/,,/+"'- M"'" *"+ '" $%& '"'" *"+ $! -/-'/3 $! '-"+"$"+ '-"+"$"+ '!/,,/+ '!/,,/+"'"'- !-/ !-/ $" &" !' -/&*3 -/&*3 '-'%! '-'%! "$/ "+! !'" $=& '!'' $" -%& $,%& $-"+" / *"+ -"/ -&"++ 7 $" > +>"* $! /! -/ $! -/-""+" $-"+" !--&" !--&" '" '" O%-& !/-" 3 !% !% '"'" '"'" -/ -/ !-/,%,+ !-/,%,+ &/ -%% $.-/&!" (N/ - %3 200@) A/ '"' %"+ $%& / *"+ '-"+%' ./,- .-'/"" ! -/ .-"*"/" U%/8,%-3 O>,"3 ." !-$"* '-%% -/+ &. 7%/ "! '-"$.!" / -/& *"+ $. $+"!" "! -/+ !-.-/%" P$ -/ -!/"+ " !-$/" '*/! "! '-"$.!" / *"+ '-'-"& */ !--&" -'!" '-""+! S-/"+ $-"+" &% -/- '! $-= " -'!" '-"#'/ .% D-., A/ M"' I U%"+ (DAMIU) *"+ '-"*-$!" / . '"' S-%" '/&3 / '"' %"+ #+ $#'. $ -/+ -'.3 -. !-'"+!"" -/ $'& !-/ H% " $-!" !/-" $! -' DAMIU '-%!!" .-"+,%&" -/ -. -. $" -"/3 '%"* '%"* !% /
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-%/&"* $%!!" -/ ,,' -&"++ $. '-'.-"+/& !% / *"+ $&%!" (A&-" - %3 200) Escherichia coli coli $"+! E. $"+! E. coli3 coli3 '-/.!" %& #-" .-- ' ' !!-/ / +/' +/' "-+ "-+7 7 *"+ *"+ -/' -/' ! ! $%' $%' 7' 7'% % E"E"-/, /, -/ -/ --33 -/-"! "+ $" $! '-'-"! .,/ E. .,/ E. coli " coli " -"++&"* -"++&"* '-/.!" '-/.!" .-"+&" ",/'% 3 -%" -/!-'"+ ! $ %"+!"+" -!/ '" K-"*!" E. K-"*!" E. coli coli $! -/&*3 -. --/. -.-/ E. -.-/ E. coli .coli .- O15:H3 $. '-"+!!" !-/"" '!"" *"+ -/ .$ '" (A/'"3 200@) Escherichia coli '-/ '-/. .!" !" ! !-/ -/ "$ "$! !,/ ,/ !% !% / / '"' '"' !/!/-" " !--/$""* $ $%' / '-"+"$!!" &= / -/- -/!,"'" ,%-& 7--3 *"+ !-'"+!"" #+ '-"+"$"+ '!/,,/+"'- -"-/! .,+-" %""* (T,/,/3 (T,/,/3 2004 200 4 dalam R$# dalam R$# - %3 2010) E. coli '-/. '-/.!" !" '!/,, '!/,,/+ /+" "''- *"+ $.! $.! -+ -+ "$ "$,/ ,/ "! "! '-"+#
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III.
III.1.
METODE PR PRAKTIKUM
Te#$at %an &akt!
P/!!' P/!!' " $%!"!" $%!"!" $ L,/,/' L,/,/' A+/,-!",%,+ A+/,-!",%,+ U" F,.,%,+ F,.,%,+ F!% P-/"" U"8-/ H% O%-, .$ &/ S-% "++% B1 M- 2016 .!% 1B00 1500 ITA
III..
Ba'an %an Alat
9&" *"+ $+"!" $%' ./!!' $-"7! !-/ -/&* .$ ##"" '"'" $%& '-$ EM9A3 %,&,%3 -%,.3 !$-3 '.-% '"'" (."3 !-$-% $" / '"-/% %"+) A% A% *"+ *"+ $+" $+"!" !" $%' $%' ./! ./!!' !' $-" $-"7! 7! !-/ !-/ -/& -/&* * .$ ##"" '"'" * laminar air flow3 flow3 %'. 9"-"3 % .-"*-/3 =" .-/3 '/,- $" '!/,..-
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Pr)*e%!r Ker"a
P/,-$ P/,-$/ / !-/# !-/# $/ $/ ./! ./!!' !' $-" $-"7! 7! !-/ !-/ -/& -/&* * .$ .$ ##" ##"" " '"'" * -+ -/! : 1. M-'"!" '-$ EM9A *"+ -%& $ --%'"*3 !-'$" "+
!-$%' =" .-/ -/% . S--%& '-$ '-'$3 $%!!" -!"! .-"+-"-/"
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I-.
I-. I-.1. Ha*l Ha*l
HASIL DAN PEMBAHASAN
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I-.. I-.. Pe#/a'a*an
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coli. 9!-/ E.coli 9!-/ E.coli '-/.!" '-/.!" !-/ *"+ "*! '-"+!,"'" '!"" $" '"'" E. '"'" E. coli '! coli '! !- $%' & '-%% ,/% $/ '"'" *"+ -/-'/ !-/ E.coli '-/.!" '-/.!" !-/ *"+ -/'! 7'% E"-/,-/E"-/,-/-- *"+ '-/ '-/.! .!" " .-"+& .-"+&" " ",/' ",/'% % % %/ /" " .-"-/ .-"-/" "" " &-= &-=" " $" $" '" '" 9! 9!-/ -/
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%!, %!, '-"+& '-"+&% %!" !" !,%," !,%," $-"+" $-"+" " " -/=/ -/=/" " +-%. +-%. $-"+" $-"+" !%. !%. %,+' %,+' S-$"+!" '!/, %" *"+ $. '& !,%,""* $! -/=/" A$"* -," $" '-%-" %- '-'" '-'.-/#' .-/-$" -/- M-$ " "+ ! "!
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EM9A EM9A '-/.! '-/.!" " '-$ '-$ .$ .$ *"+ $. $. $+"! $+"!" " "! "! '-"-" '-"-"!" !" #-" #-" !-/ ,% $-"+" '-'-/!" &% .,7 $%' "+ P/!!' $-"7! !-/ -/&* .$ '"'" ##"" $-"+" / '-'"!" '-$ EM9A *"+ -%& $ --%'"* !-'$" $"+ !-$%' =" .-/ S--%& '-$ '-'$ $%!!" -!"! .-"+-"-/" .$ '.-% *"+ !" $+"!" (/ '"-/% %"+3 !-$-% $" .") S--%& '-%!!" .-"+-"-/" -. '.-% $'% -"*! 031 '% %% $"+ !-$%' =" .-/ *"+ -/ '-$ EM9A -%"#"* $-/ '-"++"!" % -/ &"++ / $" !-/"+ S--%& $ "! -%' 4 #' H% $/ "! -%' 4 #' * .$ '.-% / '"-/% %"+3 !-$-% $" ." $%& !,%," *"+ -/-"! % !,%," -/=/" &# '-%!
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-.
PENUTUP
-.1. Ke Ke* *#$! #$!lan lan
9-/$/!" ./!!' *"+ -%& $%!!" $. $'.%!" &= $! $ !-/ E. !-/ E. coli *"+ -/$-"7! .$ -' '.-% !/-" .$ '-$ *"+ $+"!" "! '-"'&!" !-/ " $! '-"/!" $"* .-/'&" !-/ E. !-/ E. coli &% coli &% " $. $%& $/ =/" =/" !,%," *"+ '& .$ '-$ $! $ *"+ -/=/" &# '-&%!
-.. Sara Saran n
S/" *"+ $. ./!!" -/!" * $%' '-%!!" .-/!!' &/ $%!!" $-"+" -% $" -"/ -/' '-%!!" .-"*-/" $ +/ %-'.-"+ %-'.-"+" " !/-" !/-" .% .% .-"*-/ .-"*-/" " *"+ *"+ $%! $%!!" !" %& %& '! '! !,%," !,%," $! $! '& -.-/ *"+ $"+"!"
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DAFTAR PUSTAKA
A/'" 200@ Buku 200@ Buku Aar !lmu "i#i $eracunan Makanan Makanan EGC: J!/ A&-"3 S!/3 H-"$/,3 A"=/3 $" H/*,",3 200 $andungan 200 $andungan Bakteri %otal %otal Coli dan Escherichia Coli&'ecal Coli ada air minum dari deot air minum isi ulang ulang di akarta akarta,, %ange %angeran rang, g, dan Bekasi Bekasi.. P%"+ E!,%,+ K--&": J!/ M-%%=3 R& 200@ Escherichia 200@ Escherichia coli dalam $ehiduan $ehiduan 9,%rends 9,%rendsV,%4N,1 V,%4N,1 N/3 C M3 R,*$ A3 $" S'"/ S '"/ 200@ U# K"$"+" 9!-/ Esherichia 9!-/ Esherichia coli .$ A/ M"' I U%"+ $/ D-., A/ M"' I U%"+ $ K.-" R-'"+3 M-$+/,. M-$+/,. urnal !lmu*!lmu Pertanian V,% Pertanian V,% 5 N, 2 R$#3 M3 A"+% P $" A-! A-! S 2010 +eteksi 2010 +eteksi Ceat Bakteri Escherichia coli dalam Samel Samel Air Air dengan dengan Metode Metode Polyme Polymeras rasee eacti eaction on Menggu Menggunaka nakan n Primer Primer -E- dan -E/ -E/ M!/ S"3 V,% V,% 143 N, 1
