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Histolo gy Laszlo Vass, Vass, Ed.D. 42-0013-00-01
Lab Repo RepoRt
Version
assistant
This document is not meant to be a substitute for a formal f ormal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Purpo se What What is the the purp purpos ose e of this this exercise? Are there any safety concerns associated with this exercise? If so, list what they are and what precautions should be taken.
Exercise Epithelial Tissue
1:
Data Table 1: Epithelial Tissue Observations TISSUE TYPE
OBSERVATIONS Alveoli, simple squamous cells
Simple Squamous Simple Cuboidal
Basement membrane, connective tissues, simple cuboidal cells
Simple Columnar (stomach)
Basement membrane, connective tissue, simple columnar cells, microvilli, nuclei
Simple Columnar (duodenum)
Basement membrane, connective tissue, simple columnar cells, microvilli, nuclei Keratinized cells, epidermis, papillary
Stratified Squamous (keratinized) dermis, reticular dermis Stratified Squamous (non-
Stratified squamous cells, basement membrane, connective tissue Cilia, psuedostratified cells, basement
Pseudostratified Ciliated Columnarmembrane, connective tissue
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Experim
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Transitional Stratified Cuboidal (online)
Stratified cuboidal cells, connective tissue, sweat gland
Stratified Columnar (online)
Stratified columnar calls, cell nuclei, connective tissue
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Questions A. Why is the study of histology histology important in the overall overall understanding of anatomy and physiology? The study of histology histology is important important in the overall overall understanding understanding of anatomy anatomy and physiology physiology because it helps with understanding the structure and function of various tissues is important for studying organs and systems.
B.
How are epithelial tissues named? Epithelial tissues are named based on shape and arrangement.
C.
Why are some epithelial tissues stratified? Some epithelial tissues are stratified because they have multiple layers of cells as opposed to
simple tissues
which are only a single layer of cells.
D. Unlike squamous cells, cells, cuboidal and columnar cells cells have large, open cytoplasm. Which functions of epithelial tissue tissue are supported by having such big cells? Absorption, secretion and it allows rapid diffusion or transport of substances through membrane.
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Experim E.
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Look at the following following drawings drawings and identify each each type of epithelial epithelial tissue: tissue:
1. simple squamous
2. simple columnar
3. pseudostrati fied columnar
4. stratified squamous
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Exercise 2: Connective Tissue obseRvations
Data Table 2: Connective Tissue Tissue
Amount and Shape of
Amount of
Are there fibers? If so, are are the arall rallel el
Mesenchyme (online)
23- polygonal
20%
Both
28- spindle
30%
Parallel
8- polygonal
N/A
N/ A
20- polygonal
35%
Scattered
40- star shaped
30%
Scattered
Too many to count
N/A
Parallel
Too many to countround Too many to count
30%
N/ A
50%
Scattered
Too many to count
40%
Parallel
Too many to countround
90%
Parallel
Too much
N/A
Aerolar (online) Adipose (online) Dense Irregular (online) Reticular Dense Regular: Tendon Hyaline Cartilage Elastic Cartilage Fibrocartilage Compact Bone Human Blood
Too many to countcount-
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Questions A. What is the primary primary function of connective tissue? They protect, support and bind bind together the other tissues of the body.
B. What can the shape of the cells in a particular type of tissue tell tell about the function of that tissue? Some shapes refer to the way a cell looks in typical tissue sections, not to the complete threedimensional from its
shape of the cell. Cells that look one way in a tissue section section usually look completely different different if viewed upper surface.
C.
What is matrix? Why do some tissues tissues have more matrix matrix than others? Matrix is non-cellular matter between the cells. Some tissues have more matrix than
others because the cells make and extrude the matrix that surrounds them.
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D. What do collagen fibers provide? Collagen fibers allow tissues to be flexible while providing additional strength and stability.
E. Tendons, ligaments ligaments and cartilage cartilage have limited blood supply. Explain how this might affect the ability of these tissues to heal after an injury. It limits the rate at which mitosis can occur.
Exercise 3: Muscle Tissue obs eRvatio ti ons Data Table 3: Muscle Muscl Shape and Arrangement of Cells e Skelet al Smoot h
Str ia ti on s
Skeletal muscle cells, nuclei
Yes
Endomysium, cell nuclei, smooth muscle cell
No
Cell nuclei, intercalated discs, cardiac muscle cell
No
Cardia c
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Questions A. What kind of muscle muscle would would you find in the stomach? stomach? Smooth muscle would be found in the stomach.
B.
How is smooth muscle structure structure different different from that of skeletal
and cardiac muscle? Smooth muscle lacks striations and is involuntary. C.
Why is skeletal muscle voluntary? It is under voluntary control from the brain and provides
strength to move the limbs and body.
D. What is unique about cardiac muscle? Cardiac muscle is composed of a series of cells that work together as one unit and respond to electrical
impulses that allow the heart to beat.
Exercise 4: Nervous Tissue obseRvations Make a sketch of the neuron as seen in your microscope slide and label the following: Cell body, nucleus, cell processes and neuroglia.
Questions A. What is the function of nervous tissue? changes
Nervous tissue is specialized to send electrical signals that inform the central nervous system of in the environment or body.
B. Why are the cell cell bodies bodies of neurons elongated into cell processes? processes? For transmission of information. These dendrites receive signals from other cells and conduct messages to the cell body.
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Experim C.
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If all nerves respond respond to stimuli, stimuli, why cannot eyes “hear” sound and ears ears “see” light? Each nerve is designed to respond to and transmit specific stimuli from a certain area of the body.
D. How is a nerve different from a neuron? neuron? A neuron is only one part of a nerce. A neuron and neuroglia together make up a complete nerve.
Conclusions Explain the purpose of these exercises and why studying histology is important to the understanding of how the human body functions. Histology allowed us to examine the structure and composition of all of our tissues and how they are arranged into organs.
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