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biosci labFull description...
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Patrick Daniel Libiran
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POLITICAL LAW
Complete Blood Count Ex 25
Universal precaution
Treat all body fuids inectious
CBC Hemoglobin Hematocrit RBC WBC Diferential Count
Hematocrit •
•
•
•
Volume o packed red cell ater centriugation PCV dam!" micromet#od $aterial"% capillar& tube 'red( blue) "ealing cla&*para+n ,ax micro#ematocrit centriuge mac#ine micro#ematocrit reader
Procedure% -. /ill capillar& tube ,it# blood '0*1) 2. eal. 3abel 0. Centriugate. 4"e balancer 1. pin -( rpm 165min •
7R% male6 18 9*6 8 :ol; * .18 9*6 8 emale6 12 9*6 5 :ol; < 7B = 5> 9*6 :ol;
Hemoglobin •
•
•
•
•
Heme ?lobin Pigment in t#e RBC $td" acid6#ematin met#od C&anmet#emoglobin met#od $aterial"% .- 7 HCl a#li!" tube ',*comparator block) a#li!" pipette dH2 tirring rod
Procedure% -. Deli:er .-7 HCl into a#li!" tube = 2mark 2. "pirate .2 m3 blood 0. Expel blood into a#li!" tube 1. Rin"e pipette ,it# dH26add into t#e tube 5. dd dH2 drop b& drop = comparator block >. Read lo,er mini"cu" •
7R% male % -16->.5 g*-m3 or -16->.5g; -16->5 g*3 emale% -26-5g*-m3
Hemac&tometer
•
•
•
4"ed in counting RBC( WBC( Platelet( ab"olute eo"inop#il count( "perm count Wit# t#ick co:er "lip @mpro:ed 7eubauer Counting C#amber
WBC Count •
•
A o WBC in - cu mm o blood Dil uid mu"t l&e RBC
$aterial"% WBC pipette Dil uid #emac&tometer •
Procedure% -. "pirate .5 blood. Wipe "ide" 2. "pirate WBC dil uid up to -- mark. $ix dil!n -%2 no bubble"( no o:er*under dilution 0. Di"card 260 gtt 1. C#arge counting c#amber 5. tand 5min >. Count WBC in LPO •
7R%
RBC Count •
•
A o red cell" in - cu mm blood Dil uid l&e WBC
$aterial"% RBC pipette Dil uid #amac&tometer
Procedure% -. "pirate blood .5 mark. Wipe "ide" 2. "pirate RBC dil uid up to -- mark. $ix dil!n -%2 0. Di"card 56> gtt 1. C#arge counting c#amber 5. tand 5min >. Count RBC in HPO •
7R%
Diferential Count •
•
?ranuloc&te" granuloc&te"
$aterial"% Blood 2 "lide" Diferential "tain Pa"teur pipette Cedar ,ood oil •
•
•
•
Hig# reracti:e index( t#ereb& increa"ing t#e numerical aperture o t#e obecti:e len" Ha:e "pecic optical and :i"co"it& c#aracteri"tic" Co:er gla"" = t#icker( le"" exible( "urace ten"ion o t#e uid ,ill not deorm t#em( #eig#t o t#e uid i" "tandardied
Procedure -. Prepare "mear 2. Dr& "mear on at "urace 0. tain "mear 1. Count - cell" in F@F •
Strip Dierential Tecni!ue
7R%
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