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Preparation, Dispensing and Sterilizing of Media for Cultivation of Microorganism
UDEE 2114 MICROBIOLOGY YEAR 2 SEMESTER 3
Name : Hemalatha Kannan Student ID : 09ADB07509 Partners name: Ngoi Jiang Chin Hing Yi Shien Yap Ye Vone Chew Hui Sin Sanmugapriya Elamparuthi Faculty : Science Course : Biochemistry Session : 2009/2010 Lecturer : Miss Teo Kah Cheng st
Date : 1 March 2011
Title: The determination of microbial numbers
Objective(s) :1) To determine the microbial number using spread plate method.
2) To determine the number of bacteria on the plate using the dilution dilution factor.
Results Spread Plate Part A : Removal of 0.1ml from the dilution tube Dilution -1 10
Results
Observation
Confluent growth of bacteria. 4 single colonies is observed.
-2
10
Confluent growth of bacteria. Many small single colonies present.
-3
10
Many single colonies are present. The growth is still confluent.
-4
10
The number of single colonies of bacteria has reduced but the colonies are still more than 200 colonies.
-5
10
The number of single colonies is few but still it is more than 200 colonies.
Part B : Removal of 1.0ml from the dilution tube Dilution -1 10
Results
Observation
The growth of bacteria is very confluent. No single colonies present.
-2
10
The growth of bacteria is very confluent. No single colonies present.
-3
10
The growth of bacteria is very confluent. No single colonies present.
-4
10
Single colonies are present at the edge of the agar plate. The growth of bacteria is still confluent.
-5
10
Many single colonies of bacteria is present. The number of colonies present is more than 200.
Discussion
The main purpose of streak plate method is to form well-isolated colonies so that pure culture can be obtained. As a results, isolated colonies were obtained at the end of streak in each dilution. For the spread plate with 0.1ml
References
1. UDEE 2114 Microbiology Lab Manual, Experiment 1 a nd 3. 2. http://inst.bact.wisc.edu/inst/index.php. Microbiology. R etrieved on 10
th
March 2011.
3. Karren E. Messley and St ephen A.Norrell.(2003). Microbiology Laboratory Manual. Prentice Hall.