MICROBIOLOGY report 3

UDEE 2114 MICROBIOLOGY YEAR 2 SEMESTER 3

Name : Hemalatha Kannan Student ID : 09ADB07509 Partners name: Ngoi Jiang Chin Hing Yi Shien Yap Ye Vone Chew Hui Sin Sanmugapriya Elamparuthi Faculty : Science Course : Biochemistry Session : 2009/2010 Lecturer : Miss Teo Kah Cheng st

Date : 1 March 2011

Title: The determination of microbial numbers

Objective(s) :1) To determine the microbial number using spread plate method.

2) To determine the number of bacteria on the plate using the dilution dilution factor.

Results Spread Plate Part A : Removal of 0.1ml from the dilution tube Dilution -1 10

Results

Observation

Confluent growth of bacteria. 4 single colonies is observed.

-2

10

Confluent growth of bacteria. Many small single colonies present.

-3

10

Many single colonies are present. The growth is still confluent.

-4

10

The number of  single colonies of  bacteria has reduced but the colonies are still more than 200 colonies.

-5

10

The number of  single colonies is few but still it is more than 200 colonies.

Part B : Removal of 1.0ml from the dilution tube Dilution -1 10

Results

Observation

The growth of  bacteria is very confluent. No single colonies present.

-2

10

The growth of  bacteria is very confluent. No single colonies present.

-3

10

The growth of  bacteria is very confluent. No single colonies present.

-4

10

Single colonies are present at the edge of the agar plate. The growth of  bacteria is still confluent.

-5

10

Many single colonies of  bacteria is present. The number of  colonies present is more than 200.

Discussion

The main purpose of streak plate method is to form well-isolated colonies so that pure culture can be obtained. As a results, isolated colonies were obtained at the end of streak in each dilution. For the spread plate with 0.1ml

References

1. UDEE 2114 Microbiology Lab Manual, Experiment 1 a nd 3. 2. http://inst.bact.wisc.edu/inst/index.php. Microbiology. R etrieved on 10

th

March 2011.

3. Karren E. Messley and St ephen A.Norrell.(2003). Microbiology Laboratory Manual. Prentice Hall.