FAR 121/ 4 MI CROBI OLOGY FOR PHARMACY PRACTICAL REPORT 2:
A) EXAMINATION OF CULTURES FOR MORTALITY B) CULTURE TECHNIQUE
Name
: Low Kah Jun
Ma!"# No
: $%&'%(
No IC
: '*(+%*,(*,++%'
-ae
: +h O.o/e! %($+
Le.u!e!
: A00o. 1!o2 -! H3h 1a4"5ah I/!ah"m
I.
EXAMINATION OF CULTURES FOR MOTILITY
Objectives:
$ To "6en"27 an6 6"22e!en"ae /eween !ue mo"5"7 an6 B!own"an mo"on % To 2am"5"a!"4e an6 8e!2e. he"! a0e8". e.hn"9ue0 "n , han65"n a0e8". !an02e!0 , "0o5a"n a 8u!e .u5u!e Intro!ction:
A 5a!e num/e! o2 m".!oo!an"0m0 a!e mo!e o! 5e00 a.";e57 mo"5e /7 25ae55a F5ae55a a!e 5on h!ea6,5" 0u6en0 w"55 /e e#8o0e6 o % meho60 o2 6ee!m"n"n mo"5"7? one "0 9u".< /u !e9u"!e0 a 5"5e 0<"55 w"h he m".!o0.o8e> he ohe! "0 mu.h ea0"e! o 8e!2o!m /u one ha0 o a55ow %@,@ hou!0 2o! "n.u/a"on M"teri"#s: Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae + Met$o: %A&'"n(in( )ro* *re*"r"tion Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae * %+& So,t "("r -st"b Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae *
Res!#t: %A& '"n(in( ro* *re*"r"tion
O/0e!;a"on0
E#85ana"on
•
Ro6,0ha8e6 Pseudomonas aeruginosa
•
mo;e0 "n a 4"4ae6,5"
•
08e."2". 6"!e."on an6 e#h"/"0 !ue mo"5"7 The /a.e!"a a!e mo"5e 6ue o he 8!e0en.e o2 25ae55a
mo"on o2 Pseudomonas aeruginosa
Pseudomonas aeruginosa Man"2".a"on : @( $(
•
I!!eu5a! .5u0e! o2 08he!".a5 0ha8e6
•
Staphylococcus aureus e"he! ;"/!ae0 "n a 2"#e6 8o0""on o! mo;e0 !an6om57 "n an7 6"!e."on The /a.e!"a mo;e !an6om57 "n an7
•
6"!e."on 6ue o he .o55"0"on w"h a"! o! wae! mo5e.u5e0 The /a.e!"a 6o no mo;e "n a 08e."2".
•
6"!e."on I e#h"/"0 B!own"an mo;emen The /a.e!"a a!e non,mo"5e 6ue o he 5a.<"n 8!e0en.e o2 25ae55a
Staphylococcus aureus Man"2".a"on: @( $(
%+& So,t "("r -st"b
O/0e!;a"on0
E#85ana"on
•
!owh o2 Pseudomonas aerugina "0
•
08!ea6 on he 0u!2a.e o2 he 0o2 aa! an6 he 0"n5e 0a/ 5"ne D5"ne o2 "no.u5a"on) ma6e /7 he 0!a"h w"!e Ba.e!"a !owh a!ea "0 5a!e!
•
Pseudomonas aerugina "0 a mo"5e /a.e!"a
Pseudomonas aeruginosa
•
• • •
!owh o2 Staphylococcus aureus "0 5"m"e6> 3u0 a!oun6 he 0"n5e 0a/ ma6e /7 he 0!a"h w"!e on he 0o2 aa! The 0a/ 5"ne "0 ;e!7 0ha!8 an6 6e2"ne0 Ba.e!"a !owh a!ea "0 0ma55e! Staphylococcus aureus "0 a non,mo"5e /a.e!"um
Staphylococcus aureus
)isc!ssion: DA) Han"n -!o8 Meho6
Mo"5"7 o2 m".!oo!an"0m "0 one o2 he meho60 ha u0ua557 u0e6 o 0how0 he 8!e0en.e o2 he 25ae55a "n /a.e!"a Fo! e#am85e0 0o2 aa! 0a/ meho6> we,moun e.hn"9ue an6
han"n 6!o8 8!e8a!a"on an6 o/0e!;a"on un6e! he m".!o0.o8e Th"0 meho6 6oe0 no ";e he 6ea"50 "n amoun an6 a!!anemen o2 he 25ae55a /u u0e6 o 0how he 8!e0en.e o2 25ae55a The mo"5"7 .an /e 6";"6e6 "no h!ee 78e0 a0 /e5ow: $ 0how !ue mo"5"7 /7 mo;"n "n a 6e2"n"e 6"!e."on % /!own"an mo;emen 6ue o he .o55"0"on0 w"h 0u!!oun6"n 8a!".5e0 G non,mo"5"7 /7 ;"/!a"n on he 0ame 08o A2e! he han"n 6!o8 8!e8a!a"on> he 05"6e a!e 8u on he 0ae o2 m".!o0.o8e> Pseudomonas aeruginosa 0how0 he mo;emen "n 0ame an6 6e2"n"e 6"!e."on Th"0 8!o;e6 ha Pseudomonas aeruginosa 8o00e00 25ae55a o a"6 "n mo;emen The!e2o!e> he7 0how !ue mo"5"7 B7 u0"n he man"2".a"on o2 @((> mo"5"7 o2 he Pseudomonas aeruginosa .an /e o/0e!;e6 Fo! Staphylococcus aureus> " "0 mo;"n "n 6"22e!en "n6e2"n"e 6"!e."on0 an6 0ome ;"/!a"n ;"o!ou057 on he 0ame 08o Th"0 .a55e6 B!own"an mo;emen an6 "0 6ue o he .o55"0"on o2 he /a.e!"a w"h he 0u!!oun6"n 8a!".5e0 5" Staphylococcus aureus 6oe0 no 0how !ue mo"5"7 an6 a!e non,mo"5e /a.e!"a
DB) So2 aa! 0a/ Th"0 e#8e!"men "0 05ow /e.au0e !e9u"!e0 @ hou!0 "n.u/a"on o a55ow /a.e!"a !owh The 8ae!n o2 !owh o2 he .o5on"e0 0how0 he mo"5"7 o2 he /a.e!"a Th"0 meho6 "0 mu.h ea0"e! o /e .a!!"e6 ou a0 " 6oe0 no !e9u"!e 0<"55 w"h he m".!o0.o8e In a66""on> hea 0ou!.e o2 he 5"h m".!o0.o8e ma7 6!7 an6 <"55 /a.e!"a "n han"n 6!o8 meho6> !e0u5"n "n an "n.o!!e. !e0u5 Pseudomonas aeruginosa !ew a!oun6 he 0a/ 5"ne an6 on he 0u!2a.e o2 he aa! !owh 08!ea6 h!ouhou he who5e /o5e !ahe! han /e"n 2o.u0e6 a5on he 5"ne o2 "no.u5a"on Th"0 "0 /e.au0e he 8!e0en.e o2 he 25ae55a a55ow0 " o mo;e 2u!he! awa7 2!om he 0a/ 5"ne an6 08!ea6 who5e aa! Staphylococcus aureus !ew on57 a5on he 5"ne o2 "no.u5a"on w"h"n a 5"m"e6 a!ea The !owh 8ae!n "0 0ha!8 an6 6e2"ne6 Th"0 0howe6 ha " "0 non,mo"5e a0 " "0 una/5e o mo;e awa7 2!om he 0a/ 5"ne an6 08!ea6 a!oun6 he aa! me6"um 6ue o he a/0en.e o2 25ae55a Prec"!tions:
$ Boh he w"!e 5oo8 an6 he 0!a"h w"!e mu0 /e heae6 "55 !e6 ho /e2o!e an6 a2e! u0"n o 8!e;en .onam"na"on an6 /oh o2 hem mu0 /e .oo5e6 6own .om85ee57 /e2o!e a<"n he /a.e!"a 2!om he aa! 85ae o! /!oh a0 he hea wou56 <"55 he /a.e!"a
% A0e8". e.hn"9ue0 0hou56 /e .a!!"e6 ou 6u!"n he e#8e!"men G The 5"h "nen0"7 o2 he m".!o0.o8e 0hou56 /e m"n"m"4e6 o 8!e;en he /a.e!"a 2!om 6!7"n ou an6 67"n @ The .u5u!e /!oh 0hou56 /e en57 0ha he "no.u5a"n 0!a"h w"!e 0hou56 /e 0a//e6 05ow57 an6 en57 o a;o"6 he aa! 2!om /!ea<"n Conc#!sion: Han"n 6!o8 meho6 "0 2a0e! "n o/0e!;e he !e0u5 .om8a!e o 0o2 aa! 0a/ wh".h nee60 "n.u/a"on 8e!"o6 o2 %@,@ hou!0 Howe;e!> han"n 6!o8 meho6 nee60 a h"he! 0<"55 an6 me".u5ou0 8!o.e6u!e o en0u!e ha o/0e!;a"on .an /e 6one> wh"5e 0o2 aa! 0a/ "0 mu.h ea0"e! o .a!!7 ou an6 !e0u50 .an /e o/0e!;e6 o/;"ou057 an6 ea0"57 Boh e.hn"9ue0 0how .5ea!57 he mo"5"7 o2 /a.e!"a -e2"n"e an6 .on0"0en mo;emen o2 /a.e!"a "0 .on0"6e!e6 o /e !ue,mo"5"7 wh"5e "n6e2"n"e mo;emen "0 .a55e6 B!own"an mo;emen The!e2o!e> Pseudomonas aeruginosa "0 a mo"5e /a.e!"a 6ue o he 8!e0en.e o2 25ae55a Staphylococcus aureus "0 a non,mo"5e /a.e!"a
II.
CULTURE TEC'NI/UE
Intro!ction:
A0 mo0 m".!oo!an"0m0 a!e 8oen"a557 8ahoen". " "0 e00en"a5 ha he7 /e han65e6 /7 8!o.e6u!e0 wh".h a;o"6 .onam"na"on o2 he m".!o/"o5o"0 A50o 0"n.e m".!oo!an"0m0
a!e 0o u/"9u"ou0> " "0 ne.e00a!7 o a;o"6 .onam"na"on o2 m".!o/"a5 .u5u!e /7 0!a7 m".!oo!an"0m0
0& Inoc!#!1 tr"ns,er "& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 one "("r *#"te to "not$er b3 stre"4in( M"teri"#s: Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae & Met$o: Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae & b& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 "n "("r *#"te to " bott#e o, n!trient brot$ M"teri"#s: Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae Met$o: Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae
Res!#t: 0& Inoc!#!1 tr"ns,er "& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 one "("r *#"te to "not$er b3 stre"4in(
O/0e!;a"on0
E#85ana"on
•
The .o5on"e0 o2 Escherichia coli
•
a!e 08!ea6 2!om he 2"!0 0!ea<"n an6 0u..e002u557 0e8a!ae6 "no .o5on"e0 a he en6 o2 0!ea<"n The .o5on"e0 a!e o;a5 "n 0ha8e
•
an6 m"5<7 7e55ow"0h "n .o5ou! I0o5ae6 .o5on"e0 o2 Escherichia
•
coli a!e 2o!me6 S!ea<"n wa0 0u..e002u5
Escherichia coli D "n aa! 85ae)
b& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 "n "("r *#"te to " bott#e o, n!trient brot$
O/0e!;a"on0
E#85ana"on
•
Be2o!e "n.u/a"on> he /o5e o2 nu!"en /!oh "0 .5ea!> !an08a!en an6 7e55ow"0h "n .o5ou!
•
A2e! "n.u/a"on> he /o5e o2 nu!"en /!oh u!ne6 u!/"6 6ue o he !a8"6 !owh o2 Escherichia coli /e.au0e " .ona"n0 he mea/o5". 0e.!e"on o2 Escherichia coli
Escherichia coli A2e! "n.u/a"on )isc!ssion: "& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 one "("r *#"te to "not$er b3 stre"4in(. S!ea<"n "0 u0e6 o 6"5ue he .o5on"e0 o2 /a.e!"a "no 0ma55 an6 "0o5ae6 .o5on"e0 an6 mu0 /e 6one me".u5ou057 o en0u!e 0u..e002u5 "0o5a"on S!ea<"n "0 6one a 5ea0 @
"me0 "n o!6e! o o/a"n "0o5ae6 .u5u!e0 In.u/a"on ha0 o /e 6one a G& ℃ 2o! %@
o @ hou!0 -"22e!en /a.e!"a ha;e 6"22e!en .o5ou!0> e#u!e0> 0ha8e0 an6 0"4e0 The .o5on"e0 o2 Escherichia coli a!e !oun6 "n 0ha8e an6 m"5<7 7e55ow"0h "n a88ea!an.e b& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 "n "("r *#"te to " bott#e o, n!trient brot$. The nu!"en /!oh ha wa0 "n""a557 .5ea! ha6 u!ne6 u!/"6 an6 m"5<7 0how"n /a.e!"a5 !owh an6 mea/o5"e 8!o6u."on Th"0 8!o;e6 ha he !an02e! wa0 6one 0u..e002u557 Prec"!tions:
$) Hea he w"!e 5oo8 an6 he 0!a"h w"!e un"5 /u!n"n !e6 an6 a55owe6 o .oo5 0u22"."en57 /e2o!e an6 a2e! han65"n he /a.e!"a o a;o"6 .onam"na"on an6 6eah o2 /a.e!"a 6ue o hea %) O;e!5a8 e;e!7 0!ea<"n w"h he 2o55ow"n 0!ea<"n e#.e8 he 2"!0 an6 he 2"na5 0!ea< En0u!e 5a0 0!ea< an6 he 2"!0 0!ea< mu0 no /e o;e!5a8 o o/a"n "0o5ae6 .o5on"e0 G) A0 he aa! "0 a 2!a"5e me6"um> 0o he 0!ea<"n mu0 /e 6one .a!e2u557 an6 en57 @) The .o;e! o2 he 8e!" 6"0h 0hou56 /e o8ene6 a a @+an6 "0 .5o0e6 "mme6"ae57 a2e! u0e6 o m"n"m"4e .onam"na"on /7 8a!".5e0 "n he a"! +) The aa! 85ae mu0 /e "n.u/ae6 "n an "n;e!e6 8o0""on Th"0 "0 o 8!e;en he ;a8ou! 2o!me6 on he .o;e! o2 he 8e!" 6"0h /7 e;a8o!a"on an6 .on6en0a"on o2 wae! o 6!o8 on he /a.e!"a .o5on"e0 an6 a22e. he !owh *) The o8en 0"6e o2 he /o5e mu0 /e 25ame6 /e2o!e an6 a2e! han65"n /a.e!"a &) A55 he !an02e!!"n 8!o.e6u!e0 0hou56 /e 6one nea! an6 a!oun6 he 25ame a0 he a!ea "0 a00ume6 o /e 0e!"5e
Conc#!sion:
I0o5a"on o2 .o5on"e0 .an /e 6one /7 0!ea<"n meho6 Daa! 85ae) an6 "n.u/a"on o2 aa! me6"um D/o5e) The "0o5ae6 .o5on"e0 on aa! 85ae an6 u!/"6"7 o2 nu!"en /!oh .an /e u0e6 o e#am"ne !owh o2 /a.e!"a Escherichia coli we!e 0u..e002u557 !an02e!!e6 2!om one aa! 85ae o anohe! an6 nu!"en /!oh
2& Iso#"tin( " *!re c!#t!re ,ro1 " 1i5e c!#t!re M"teri"#s: Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae ' Met$o:
Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae '
Res!#t: "& Fro1 " 1i5e c!#t!re *#"te to " *"rtitione "("r *#"te
O/0e!;a"on0
E#85ana"on •
M"#e6 .u5u!e o2 Bacillus subtilis an6 Staphylococcus aureus.
•
The .o5on"e0 o2 Bacillus subtilis a!e 08he!".a5 "n 0ha8e> 0ma55e! an6 wh"e D.!eam7) "n .o5ou!
•
The .o5on"e0 o2 Staphylococcus aureus a!e 0ma55> !oun6e6> 7e55ow an6 m"5<7
M"#e6 .u5u!e o2 Bacillus subtilis an6 Staphylococcus aureus .o5on"e0
b& Fro1 " 1i5e c!#t!re *#"te to n!trient brot$
O/0e!;a"on0
E#85ana"on
•
Be2o!e
"n.u/a"on>
he
nu!"en
/!oh wa0 .5ea! 6ue o a/0en.e o2 /a.e!"a5 !owh •
A2e! "n.u/a"on> he nu!"en /!oh u!ne6 .5ou67 an6 u!/"6 6ue o /a.e!"a5 !owh an6 mea/o5"e o2 Bacillus subtilis an6 Staphylococcus aureus.
•
T!an02e! o2 /a.e!"a
wa0 6one
0u..e002u557 A2e! "no.u5a"on Bacillus subtilis an6 Staphylococcus aureus
)isc!ssion:
S!ea<"n "0 a 8!o8e! an6 0u"a/5e meho6 o "0o5ae /a.e!"a .o5on"e0 A 0u..e002u5 0!ea<"n w"55 7"e56 .o5on"e0 o2 /a.e!"a 2!om 6"22e!en 08e."e0 A m"#e6 .u5u!e o2 Bacillus subtilis an6 Staphylococcus aureus wa0 0e8a!ae6 0u..e002u557 u0"n he 0!ea<"n e.hn"9ue on a nu!"en aa! 85ae The "0o5ae6 8u!e .o5on"e0 o2 Bacillus subtilis a!e wh"e D.!eam7) "n .o5ou! an6 ."!.u5a! The "0o5ae6 8u!e .o5on"e0 o2 Staphylococcus aureus a!e m"5<7 7e55ow "n .o5ou! an6 !oun6e6 The 6"22e!en.e0 a!e o/;"ou0 an6 ;"0"/5e /7 na " "0 ha!6 o "0o5ae a 8u!e .u5u!e 2!om a m"#e6 .u5u!e /7 u0"n nu!"en /!oh /e.au0e " "0 no ea07 o 6ee. he .onam"na"on "n /!oh .u5u!e A2e! !an02e!!"n m"#e6 .u5u!e "no a nu!"en /!oh an6 "n.u/ae6> he nu!"en /!oh u!ne6 2!om .5ea! 7e55ow o u!/"6 an6 .5ou67 The u!/"6"7 wa0 .au0e6 /7 he mea/o5"e 8!o6u.0 o2 he /a.e!"a> "n6".a"n !owh o2 /a.e!"a The m"#e6 .u5u!e wa0 0u..e002u557 !an02e!!e6 /u no "0o5a"on wa0 o/0e!;e6 Th"0 0how0 ha h"0 meho6 "0 no 0u"a/5e 2o! "0o5a"n .o5on"e0 o2 /a.e!"a /u "0 0u"a/5e 2o! .u5";a"n /a.e!"a
Prec"!tions:
$ The w"!e 5oo8 mu0 /e 0e!"5"4e6 /7 hea"n un"5 /u!n"n !e6 e;e!7 "me /e2o!e han65"n /a.e!"a o a;o"6 .onam"na"on o2 he w"!e 5oo8
% Su22"."en "me mu0 /e ";en o .oo5 he w"!e 5oo8 /e2o!e han65"n /a.e!"a o 8!e;en /a.e!"a 6eah /7 hea G E;e!7 0!ea<"n mu0 o;e!5a8 he 2o55ow"n 0!ea<"n e#.e8 he 2"!0 an6 he 2"na5 0!ea< The 5a0 0!ea< mu0 no o;e!5a8 he 2"!0 0!ea< o o/a"n "0o5ae6 .o5on"e0 @ S!ea<"n mu0 /e 6one .a!e2u557 an6 en57 a0 he aa! "0 a 2!a"5e me6"um + The 8e!" 6"0h 0hou56 /e o8ene6 a a @+ 6e!ee an5e an6 "0 .5o0e6 "mme6"ae57 a2e! /a.e!"a a!e o/a"ne6 o a;o"6 .onam"na"on /7 8a!".5e0 "n he a"! * The aa! 85ae mu0 /e "n.u/ae6 "n an "n;e!e6 8o0""on o 8!e;en he e;a8o!a"on an6 2o!ma"on o2 ;a8ou! on he .o;e! o2 he 8e!" 6"0h an6 .on6en0a"on o2 wae! o 6!o8 on he /a.e!"a .o5on"e0 an6 6"0u!/ he .o5on"e0 & The mouh o2 he /o5e mu0 /e 25ame6 /e2o!e an6 a2e! han65"n /a.e!"a A55 he !an02e!!"n 8!o.e6u!e0 0hou56 /e 6one nea! an6 a!oun6 he 25ame a0 he a!ea "0 a00ume6 o /e 0e!"5e
Conc#!sion:
S!ea<"n /a.e!"a 2!om a m"#e6 .u5u!e on an aa! 85ae 0e8a!ae57 .an /e u0e6 o "0o5ae Bacillus subtilis an6 Staphylococcus aureus. The .o5on"e0 o2 he wo 08e."e0 .an /e 6"22e!en"ae an6 o/a"ne6 0e8a!ae57 Staphylococcus aureus a!e 0ma55> !oun6e6> m"5<7 an6 7e55ow? wh"5e Bacillus subtilis a88ea!0 a0 08he!".a5 an6 wh"e D.!eam7) "n .o5ou! Nu!"en /!oh .an /e u0e6 o !owh /a.e!"a> /u " "0 no 0u"a/5e 2o! "0o5a"on o2 /a.e!"a a0 a55 he .o5on"e0 w"55 /e m"#e6 "n he /!oh Th"0 e.hn"9ue wa0 0u..e002u557 8e!2o!me6
Res!#ts: Part 1
a. Observe the streaked plate and make a sketch to show the distribution of growth. 15ea0e !e2e! o he o/0e!;a"on o2 "no.u5um !an02e! "n e.hn"9ue0 o2 !an02e!!"n .u5u!e 2!om one aa! 85ae o anohe! /7 0!ea<"n / Were you able to streak properly so as to obtain isolated? re the colonies similar to those on the original plate? Ye0> I am a/5e o 0!ea< 8!o8e!57 0o a0 o o/a"n "0o5ae6 .o5on"e0 The "0o5ae6 .o5on"e0 o2 Escherichia coli o/0e!;e6 a!e 0"m"5a! o ho0e on he o!""na5 85ae The .o5on"e0 a!e !oun6 "n 0ha8e an6 no !an08a!en . Observe the changes in the nutrient broth before and after inoculation. Why did the inoculated broth turn turbid? Be2o!e "no.u5a"on> he nu!"en /!oh "0 .5ea! an6 !an08a!en an6 " /e.ome0 u!/"6 a2e! "no.u5a"on Th"0 "0 6ue o he !owh o2 Escherichia coli "n he nu!"en /!oh an6 he /a.e!"a 0e.!ee wa0e 8!o6u.0 o2 mea/o5"0m 6u!"n "0 !owh
6 !an you use a li"uid medium to isolate a pure culture? E#plain. No> we .anno u0e a 5"9u"6 me6"um o "0o5ae a 8u!e .u5u!e /e.au0e " "0 no a 0u"a/5e meho6 e .anno 6ee!m"ne whehe! he u!/"6"7 o2 he /!oh "0 .au0e6 /7 a 8u!e .u5u!e o! he .onam"na"on o2 ohe! m".!oo!an"0m0
Part 2
a. Observe the streaked plate. Were you able to separate the $ organisms successfully? %f not& why? 'escribe the morphology of the colonies produced( )colour& te#ture& shape etc. The m"#e6 .u5u!e o2 Bacillus subtilis an6 Staphylococcus aureus a!e 0u..e002u557 0e8a!ae6 The .o5on"e0 o2 Bacillus subtilis a!e 08he!".a5 "n 0ha8e> 0ma55e!> 0mooh "n a88ea!an.e an6 wh"e D.!eam7) "n .o5ou!> wh"5e he .o5on"e0 o2 Staphylococcus aureus a!e 08he!".a5 "n 0ha8e> /"e!> !ouh "n a88ea!an.e an6 7e55ow"0h "n .o5ou! / *ow would you know if your culture has been contaminated? e w"55 .o5ou!> e#u!e a0 we55 a0 0ha8e . E#plain why you must incubate an inoculated plate in an inverted position. Ba.e!"a w"55 .a!!7 ou !e08"!a"on wh".h !e5ea0e0 .a!/on 6"o#"6e an6 wae! un6e! G&
℃
E;a8o!a"on o2 wae! .au0e0 wae! 6!o85e0 D;a8ou!) 2o!me6 a he .o;e! I2 he "no.u5ae6 85ae wa0 no 85a.e6 "n an "n;e!e6 8o0""on> he wae! 6!o85e0 w"55 6!"8 ono he 0u!2a.e o2 aa! Th"0 w"55 a22e. he 2o!ma"on o2 .o5on"e0 o2 /a.e!"a /e.au0e he wae! w"55 .au0e he /a.e!"a .o5on"e0 o /"n6 oehe! a0 a me6"um 2o! mo;emen o2 /a.e!"a> 5ea6"n o an "na..u!ae !e0u5
