PHILIPPINE ASSOCIATION OF SCHOOLS OF MEDICAL TECHNOLOGY AND PUBLIC HEALTH Prototype Course Syllabus
Course Title: Clinical Chemistry 1
Course Description: The course deals with the quantitative measurement of biochemical substances found in body fluids essentially blood. This involves the knowledge and understanding of the basic concepts and principles of their metabolism, laboratory analyses, and pathophysiology. Quality assurance and safet y are given due emphasis.
Course Credit: 4 units (3 units lecture, l ecture, 1 unit laboratory)
Contact Hours: 3 hours lecture, 3 hours laboratory l aboratory per week (54 hours lecture and 54 hours laboratory per semester)
Prerequisites: Biochemistry, Human Anatomy and Physiology st
Placement: Third Year, 1 Semester
Terminal Competencies: At the end of this course, the student is able to: 1. explain the different biochemical substances, their their metabolism, actions, reference ranges and variables that may affect their analyses 2. correlate test results with pathologic conditions 3. apply concepts and principles of instrumentation in the laboratory 4. perform basic laboratory calculations 5. practice quality assurance and laboratory safety 6. perform correctly correctly laboratory laboratory assays used to measure concentration concentration of specific analytes 7. solve common problems encountered in the clinical laboratory 8. manifest professionalism
References: 1. Anderson, Shauna and Susan Cockyane. Clinical Chemistry: Chemistry: Concepts and Applications. USA: Waveland Press Inc., Inc., 2007. 2. Arneson, W. and J. Brickell. Clinical Chemistry: Chemistry: A Laboratory Laboratory Perspective. USA: F.A. Davis Co., 2007. th 3. Ashwood E., D. Bruns and C. Burtis. Tietz’s Fundamentals of Clinical Chemistry 6 ed. Philadelphia: W.B. Saunders Co., 2007. th 4. Ashwood E., D. Bruns and C. Burtis. Burtis. Tietz’s Textbook Textbook of Clinical Chemistry and Molecular Diagnostics 4 ed. Philadelphia: W.B. Saunders Co., 2007. th 5. Bishop, Michael L. et.al. et.al. Clinical Chemistry: Chemistry: Principles, Procedures, Correlation’s, 5 ed. Philadelphia: Lippincott Williams, Philadelphia, 2005. PASMETH, Inc.
6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20.
th
Crook, Martin. Clinical Chemistry and Metabolic Metabolic Medicine Medicine 7 ed. USA: Hodder Arnold Publication, 2006. th Furr, Keith. CRC Handbook of Laboratory Safety 5 ed. USA: CRC, 2000. th Garza, Diana and Kathleen Becan-McBride. Phlebotomy Phlebotomy Handbook: Blood Collection Essentials 7 ed. USA: Prentice Hall, 2004. Helms, Joel R. Mathematics for Medical and Clinical Laboratory Professionals. USA: Delmar Learning, 2008. Hibbert, Brynn. Quality Assurance in the Analytical Chemistry Laboratory. USA: Oxford University Press, 2007. Hoeltke, Lynn. Phlebotomy: Principles and Procedures. USA: Delmar Learning, 2006. th Kaplan, A., A. Pesce and S. Kazmierczak. Clinical Chemistry: Theory, Analysis, Correlation 4 ed. Edinburgh: Mosby, 2002. Lewandrowski, Kent. Clinical Chemistry: Laboratory Management and Clinical Correlations. USA: Lippincott Williams and Wilkins, 2002. th Marshall, William and Stephen Bangert. Clinical Chemistry 5 ed. Edinburgh: Mosby, 2004. nd McClatchey, Kenneth. Clinical Laboratory Medicine Medicine 2 ed. USA: Lippincott Williams and Wilkins, 2002. st Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 ed. Philadelphia: Elsevier Inc., 2007. Prichard, Elizabeth and Victoria Barwick. Quality Assurance in Analytical Chemistry. USA: Wiley-Interscience, 2007. th Sacher, Ronald and Richard McPherson. Widmann’s Clinical Interpretation of Laboratory Tests 11 ed. Thailand: F.A. Davis, 2000. nd Scott M., A. Gronowski and C. Eby. Tietz’s Applied Laboratory Medicine 2 ed. USA: Wiley-Liss, 2007. th Wu, Allan. Tietz’s Clinical Guide to Laboratory Tests 4 ed. Philadelphia: W.B. Saunders, 2006.
Electronic References: 1. http://webpages.chhs.niu.edu/williams/AHP318/ClinicalChem.htm 2. http://www.dgrhoads.com/links.shtml 3. http://www.kmcsystems.com/invitro_clinical.asp#1 4. http://www.ualberta.ca~intd410/departments/spoc.html 5. http://www2.apsu.edu/~thompsonj/clin-chem-page1.htm
PASMETH, Inc.
and external programs
quality
control
4.
5. 6. 7. 8.
At the end of this unit, the student is able to: 1. Discuss the concepts & principles of instrumentation 2. Enumerate the components of each instrument and their uses 3. Differentiate instruments according to components, analytes measured & uses; operations 4. Explain correctly proper specimen collection, handling and transport according to tests requested 5. Use spectrophotometer, UV-Vis & IR spectrophotometer 6. Discuss the principles & concepts & advantages of automation 7. Classify the different types of automation used in Clinical Chemistry
3.1.3. Mode 3.1.4. SD 3.1.5. CV 3.1.6. Variance 3.1.7. SEM 3.2. Inferential 3.2.1. t tests 3.2.2. F tests Quality Control Charts 4.1. Levey-Jennings 4.2. Westgard Rules 4.3. Six-sigma 4.4. Others Intra-laboratory QC Monitoring Proficiency Testing Method Selection Issues and Concerns
Case presentation
Laboratory: 1. Preparation and interpretation of QC chart 2. Application of Westgard Rules VI. Instrumentation Lecture: 1. Methods 1.1. Photometry 1.2. Spectrophotometry 1.2.1. AAS 1.2.2. FES 1.2.3. Reflectance Spectrophotometry 1.3. Nephelometry 1.4. Turbidimetry 1.5. Fluorometry 1.6. Electrophoresis 1.7. Chromatography 1.8. Ultracentrifugation 1.9. Chemiluminiscence 1.10. Electrochemistry 1.11. Immunochemistry 1.12. Dry Chemistry 2. Automation 3. Point of Care Testing 4. Current Trends
6 hrs
Laboratory: 1. Operation and maintenance of Spectrophotometer and other laboratory
6 hrs
8 hrs
Large group discussion
Spectrophotometer Reading
Assignment
Use of available automated machines
Technical skills Troubleshooting skills
Patience Accountability Responsibility Adherence to standards of operation
Quiz Recitation Return Demo Practical Exams Performance Checklist
Calibration of instruments Preventive maintenance of laboratory instruments
PASMETH, Inc.
of laboratory methods of lipid & lipoproteins 6. Enumerate the reference value of each lipid measured 7. Correlate laboratory results with patients lipid or lipoprotein status 8. Relate the the laboratory laboratory data in the assessment of risk or coronary heart disease (CHD) 9. Discuss the significance played by cardiac proteins and enzymes in the diagnosis of heart diseases 10. Perform correctly laboratory methods of lipid determination 11. Demonstrate safely safely precautions during performance of tests
4.
5.
Clinical Significance 4.1. Hyperlipidemia 4.2. Hyperlipoproteinemia Lipoprotein Phenotyping
Laboratory: 1. Methods for Lipid and Lipoprotein measurement 2. Laboratory Safety 3. Laboratory Waste Management
6 hrs
PASMETH, Inc.
Technical Working Group for Prototype Course Syllabi Development De velopment (2007-2008)
Zennie Aceron Petrona Benitez Sergia Cacatian Zenaida Cajucom Edwin Cancino Jacinta Cruz De Carlos Leon Oliver Shane Dumaoal Bernard Ebuen Nini Lim
Frederick Llanera Carina Magbojos Gregorio Martin Fe Martinez Josephine Milan Ferdinand Mortel Magdalena Natividad Rodolfo Rabor Ma. Teresa Rodriguez Celia Roslin
Anacleta Valdez Rowen Yolo and other PASMETH members not cited in this page who in one way or another has contributed greatly to the success of this endeavor…
PASMETH, Inc.
